An indigenous Streptomyces isolate CTF9, exhibiting promising antifungal activity against Mucor miehei and Candida albicans in pre-screening studies, was investigated by cultivation in a 50-L fermenter and by subsequent isolation, purification, and structure elucidation of the active metabolites. Based on the morphological, biochemical, and physiological characterization, as well as the 16S rRNA gene sequence, the isolate CTF9 was identified as Streptomyces malachitofuscus. Using a series of chromatographic techniques, two pure compounds were isolated from the obtained extracts after the fermentation of the isolate CTF9. The isolated compounds were identified as phenylacetic acid and indolyl-3-lactic acid by mass spectrometry (MS) and NMR analysis. The culture optimization studies revealed that the isolate CTF9 can use a variety of low-cost carbon and nitrogen sources to generate the maximum quantity of industrially important metabolites at an elevated temperature of 35°C and at a pH 7.8.

During our search program for bioactive compounds from Egyptian marine sources, we isolated a spatane diterpene tetraol (1), fucosterol (2) and linoleic acid (3) from the brown alga Sargassum subrepandum (Forsk) C.Ag. In addition to those compounds, the four hydrocarbons were detected by using GC-MS named heptadecane, 2,6,10,14-tetramethyl-hexadecane, nonadecane and heneicosane. The chemical structure of 1 is assigned here definitely to first time by spectroscopic analyses including mass spectrometry (EI-MS, HR/EIMS), 1D and 2D NMR experiments. Phytochemical study of the unsaponified fraction of the algal extract and analysis by GC-MS confirmed the existence of nine compounds, among them 4,4,7a-trimethyl-5,6,7,7a-tetrahydrobenzofuran-2[4H]-one (6). In this article, we report as well the biochemical composition of Sargassum subrepandum (including ash, fibre, lipid, carbohydrate, total protein, and amino acids compositions) together with biological activities of its extract and the isolated compounds using diverse assays.

Dentro de nuestro programa de compuestos bioactivos de fuentes marinas Egipcias, en este trabajo aislamos tetraol (1 ), fucosterol (2) y ácido linoleico (3) del alga café Sargassum subrepandum (Forsk) C.Ag. También detectamos por CG-EM cuatro hidrocarburos llamados heptadecano, 2,6,10,14-tetrametil-hexadecano, nonadecano y heniocosano. La estructura química definitiva de 1 se asignó aquí por primera vez por el análisis espectrométrico (EM-IE, AD/EMIE) y espectroscópico de RMN con experimentos de1D y2D. El estudio fitoquímico de la fracción no saponificable del extracto de la alga y el análisis por CG-EMconfirmó la existencia de nueve compuestos, entre ellos4,4,7a-trimethyl-5,6,7,7a-tetrahydrobenzofuran-2(4H)-one (6). En este artículo también reportamos la composición bioquímica de Sargassum subrepandum (que incluye cenizas, fibra, lípidos, carbohidratos proteínas totales y composición de aminoácidos) y la actividad biológica de sus extractos y compuestos aislados utilizando diferentes ensayos.