In silico and in vitro tests can reveal promising anti-leishmania activity for natural products and their derivatives. The aim of this study was to investigate in silico the pharmacological activities of potential new chalcones and their leishmanicidal potential in vitro. The in silico study was carried out using the PASS, MolPredictX and Molegro Virtual Docker 6.0 programs. Antiparasitic activity was assessed in axenic promastigote and amastigote forms of Leishmania braziliensis. The cytotoxicity tests used the J77G8 cell line. The chalcones exhibited 50% cytotoxic concentration values (CC50) values > 50 μM. Chalcone 4 (named FERAI) presented the best activity with concentration for 50% of promastigotes and intracellular parasites forms (EC50) of 9.75 ± 1.7 and 10.13 ± 1.7 μM for promastigote and amastigote, respectively. Reactive oxygen species (ROS) testing presented increased ROS levels in the parasite at the FERAI concentrations of 10 μM (56.33%), 20 μM (61.76%) and 30 μM (67.13%). Molecular docking revealed interactions (binding energy) between FERAI and the enzymes UDP-glycosyl pyrophosphorylase (-56.8384), dihydroorotate-dehydrogenase (-132.276) and trypanothione-reductase (-151.281). Our results demonstrated the anti-leishmanial activity of chalcones, especially FERAI, with a noted raising of ROS levels in the parasite. Molecular docking revealed dihydroorotate dehydrogenase and trypanothione reductase as potential pharmacological targets for FERAI. antileishmania anti leishmania derivatives PASS 60 6 0 6. programs braziliensis JG J G J77G line CC50 CC (CC50 5 named EC50 EC (EC50 975 9 75 9.7 17 1 7 1. 1013 13 10.1 respectively (ROS 56.33%, 5633 56.33% , 56 33 (56.33%) 2 61.76% 6176 61 76 (61.76% 3 67.13%. 6713 67.13% . 67 (67.13%) binding energy UDPglycosyl UDP glycosyl 56.8384, 568384 56.8384 8384 (-56.8384) dihydroorotatedehydrogenase 132.276 132276 132 276 (-132.276 trypanothionereductase 151.281. 151281 151.281 151 281 (-151.281) antileishmanial leishmanial CC5 (CC5 EC5 (EC5 97 9. 101 10. 563 56.33 (56.33% 61.76 617 (61.76 671 67.13 (67.13% 56838 56.838 838 (-56.8384 132.27 13227 27 (-132.27 15128 151.28 15 28 (-151.281 (CC (EC 56.3 (56.33 61.7 (61.7 67.1 (67.13 5683 56.83 83 (-56.838 132.2 1322 (-132.2 1512 151.2 (-151.28 56. (56.3 61. (61. 67. (67.1 568 56.8 8 (-56.83 132. (-132. 151. (-151.2 (56. (61 (67. (-56.8 (-132 (-151. (56 (6 (67 (-56. (-13 (-151 (5 ( (-56 (-1 (-15 (-5 (-

RESUMO Na agricultura irrigada, é comum a aplicação de água no solo cultivado sem critério, resultando em uso ineficiente ou não otimizado da água. Em muitos casos, a relação solo-água-planta, bem como as potencialidades e limitações dos sistemas de irrigação são ignoradas. A agricultura irrigada sustentável requer o desenvolvimento de um sistema de monitoramento da umidade do solo que evite a perda de água e melhore a eficiência do uso. O objetivo deste estudo foi desenvolver e avaliar a eficiência de um sistema inteligente de monitoramento da umidade do solo. O sistema é composto por duas estações: a primeira coleta os dados sobre parâmetros de umidade aparente do solo usando sensores, enquanto a segunda transmite esses dados para uma estação de processamento central. O sistema determina precisamente os valores atuais de umidade do solo, permitindo estimar o volume de água de irrigação necessário para atender à demanda hídrica da cultura com base na capacidade de campo. Os resultados da curva de calibração dos sensores indicam que o sistema pode medir com precisão a umidade do solo atual, auxiliando no manejo da irrigação. Para áreas irrigadas em condições de solo não saturado, recomenda-se o uso de tensiômetros devido à sua maior confiabilidade entre a capacidade de campo e o ponto de murcha permanente, garantindo práticas de irrigação mais precisas. critério casos soloáguaplanta, soloáguaplanta planta, planta solo-água-planta ignoradas estações central atual saturado recomendase recomenda se permanente precisas

ABSTRACT Water application to cultivated soil is often done without careful consideration in irrigated agriculture, leading to inefficient or suboptimal water usage. In many instances, the intricate relationship between soil, water, and plants, as well as the potential and limitations of irrigation systems, is overlooked. Sustainable irrigated agriculture necessitates the development of a soil moisture monitoring system that curtails water loss and enhances overall efficiency. This study aimed to develop and assess the efficiency of an intelligent system for monitoring soil moisture. The system comprises two stations: the first collects data on apparent soil moisture parameters using sensors, while the second transmits this data to a central processing station. The system precisely determines current soil moisture values, enabling estimation of the required irrigation water volume to meet the crop’s water demand based on field capacity. Results from the calibration curve of the sensors indicate that the system can measure current soil moisture precisely, aiding in irrigation management. For irrigated areas under unsaturated soil conditions, it is recommended to use tensiometers due to their higher reliability between field capacity and permanent wilting point, ensuring more accurate irrigation practices. usage instances plants systems overlooked stations station values crops crop s management conditions point practices

RESUMO O feijão-caupi tem grande importância socioeconômica para o semiárido brasileiro, porém sofre perdas significativas de produção pelo estresse por seca, necessitando de estratégias de manejo para superar esse cenário. Nessa perspectiva, o presente estudo teve como objetivo avaliar o crescimento e a produção de genótipos de feijão-caupi sob estresse hídrico em diferentes sistemas de cultivo. O delineamento estatístico adotado foi o de blocos casualizados, em arranjo fatorial 2 × 4 × 2, correspondendo a dois genótipos de feijão-caupi (BRS Pujante e Costela de Vaca), quatro níveis de evapotranspiração da cultura (40, 60, 80 e 100%) e dois sistemas de cultivo (com e sem cobertura morta do solo). O genótipo de feijão-caupi BRS Pujante apresentou maior área foliar e peso de 100 grãos do que o genótipo Costela de Vaca em todos os níveis de evapotranspiração da cultura. Os parâmetros de produção do feijão-caupi apresentaram os melhores resultados a 80 e 100% da evapotranspiração da cultura. Independentemente do genótipo, a cobertura morta do solo proporcionou maiores valores de peso de 100 grãos de feijão-caupi. A cobertura do solo combinada com 80% da evapotranspiração da cultura é uma estratégia adequada para a produção de feijão-caupi. feijãocaupi feijão caupi brasileiro seca cenário perspectiva casualizados Vaca, , Vaca) 40, 40 (40 60 8 solo. . solo) 10 feijãocaupi. caupi. (4 6 1 (

ABSTRACT Cowpea is a crop of high socioeconomic importance for the semi-arid region of Brazil, although facing significant yield losses due to drought, requiring management strategies to overcome this scenario. From this perspective, this study aimed to evaluate the growth and yield of cowpea genotypes under drought in different cultivation systems. The statistical design was randomized blocks arranged in a 2 × 4 × 2 factorial scheme corresponding to two cowpea genotypes (BRS Pujante and Costela de Vaca), four levels of crop evapotranspiration (40, 60, 80, and 100%), and two cultivation systems (with and without soil mulching). The BRS Pujante showed a higher leaf area and 100-grain weight than Costela de Vaca under all levels of crop evapotranspiration. The cowpea yield parameters showed the best results at 80 and 100% crop evapotranspiration. Regardless of the genotype, soil mulching provided higher cowpea 100-grain weight values. Soil mulching combined with 80% crop evapotranspiration is an appropriate strategy for cowpea production. semiarid semi arid Brazil scenario perspective Vaca, , Vaca) 40, 40 (40 60 100%, 100 100%) mulching. . mulching) 100grain grain 8 genotype values production (4 6 10 ( 1

RESUMO Introdução: Problemas renais podem ser devido apenas ao baixo peso ao nascer ou podem ocorrer em associação com outras condições. O objetivo deste estudo foi avaliar a associação entre características maternas e de nascimento, medidas antropométricas e déficit da função renal em bebês de baixo peso ao nascer. Métodos: Estudo transversal com crianças que nasceram com peso < 2500 gramas e estavam sob acompanhamento ambulatorial. Os fatores maternos investigados foram cuidados pré-natal e presença de hipertensão, diabetes e infecção durante a gravidez. As variáveis das crianças foram sexo, idade gestacional, peso ao nascer, índice Apgar, uso de medicamentos nefrotóxicos, idade, peso corporal no momento da avaliação, altura e dosagens séricas de creatinina e cistatina C. A taxa de filtração glomerular (TFG) foi estimada com a equação combinada de Zapittelli. Utilizou-se um modelo de regressão logística multivariada para identificação de fatores associados, com déficit da função renal (TFG < 60 mL/min/1,73 m2) como variável dependente. Resultados: Das 154 crianças avaliadas, 34,42% apresentaram déficit da função renal. A maioria tinha idade gestacional > 32 semanas (56,6%), peso médio ao nascer de 1439,7 gramas, e TFG média estimada de 46,9 ± 9,3 mL/min/1,73 m2. Houve uma associação significativa da TFG < 60 mL/min/1,73 m2 com o peso atual das crianças e o uso de medicamentos nefrotóxicos. Discussão: Crianças nascidas com baixo peso apresentaram alta prevalência de déficit da função renal e o peso atual normal foi um fator de proteção, enquanto o uso de medicamentos nefrotóxicos durante o período perinatal aumentou a chance de déficit renal. Estes achados reforçam a necessidade de avaliar a função renal destas crianças, especialmente aquelas que usam medicamentos nefrotóxicos. Introdução condições nascimento Métodos 250 ambulatorial prénatal pré natal hipertensão gravidez sexo Apgar avaliação C Zapittelli Utilizouse Utilizou se associados 6 mLmin173 mLmin mL min 1 73 mL/min/1,7 m dependente Resultados 15 avaliadas 3442 34 42 34,42 3 56,6%, 566 56,6% , 56 (56,6%) 14397 1439 7 1439, 469 46 9 46, 93 9, Discussão proteção 25 mLmin17 mL/min/1, 344 4 34,4 56,6 5 (56,6% 143 2 mLmin1 mL/min/1 34, 56, (56,6 14 mL/min/ (56, mL/min (56 (5 (

ABSTRACT Introduction: Kidney problems may be due to low birth weight alone or may occur in association with other conditions. The objective this study was to evaluate the association between maternal and birth characteristics, anthropometric measurements, and kidney function deficit in low birth weight infants. Methods: Cross-sectional study with children who were born weighing < 2500 grams and were under outpatient follow-up. Maternal factors investigated were prenatal care and presence of hypertension, diabetes, and infection during pregnancy. The children’s variables were sex, gestational age, birth weight, Apgar score, use of nephrotoxic medications, age, body weight at the time of evaluation, height, and serum creatinine and cystatin C dosages. The glomerular filtration rate (GFR) was estimated with the combined Zapittelli equation. Multivariate logistic regression model was used for identification of associated factors, with renal function deficit (GFR < 60 mL/min/1.73 m2) as the dependent variable. Results: Of the 154 children evaluated, 34.42% had kidney function deficit. Most of them had a gestational age > 32 weeks (56.6%), a mean birth weight of 1439.7 grams, and mean estimated GFR of 46.9 ± 9.3 mL/min/1.73 m2. There was a significant association of GFR < 60 mL/min/1.73 m2 with children’s current weight and use of nephrotoxic drugs. Discussion: Children born with low birth weight had a high prevalence of kidney function deficit and current normal weight was a protective factor while the use of nephrotoxic drugs during perinatal period increased the chance of kidney deficit. These findings reinforce the need to evaluate the kidney function in these children, especially those who use nephrotoxic drugs. Introduction conditions characteristics measurements infants Methods Crosssectional Cross sectional 250 followup. followup follow up. up follow-up hypertension diabetes pregnancy childrens s sex score medications evaluation height dosages equation 6 mLmin173 mLmin mL min 1 73 mL/min/1.7 m variable Results 15 evaluated 3442 34 42 34.42 3 56.6%, 566 56.6% , 56 (56.6%) 14397 1439 7 1439. 469 46 9 46. 93 9. Discussion 25 mLmin17 mL/min/1. 344 4 34.4 56.6 5 (56.6% 143 2 mLmin1 mL/min/1 34. 56. (56.6 14 mL/min/ (56. mL/min (56 (5 (

Resumo O conteúdo de dopamina nas estruturas cerebrais tem sido relacionado à excitabilidade neuronal e várias abordagens têm sido utilizadas para estudar este fenômeno durante o período de vulnerabilidade às crises. No presente trabalho, descrevemos os efeitos da depleção de dopamina após a administração de 6-hidroxidopamina (6-OHDA) na região pars compacta da substância negra de ratos submetidos ao modelo de epilepsia com pilocarpina. A susceptibilidade ao estado de mal epiléptico induzido pela pilocarpina, bem como a frequência de crises espontâneas e recorrentes durante o período crônico do modelo foi determinada. Sendo o hipocampo uma das principais estruturas afetadas no desenvolvimento desse modelo experimental de epilepsia, os níveis de dopamina nessa região foram determinados após a administração da droga. No primeiro experimento, 62% (15/24) dos ratos pré-tratados com 6-OHDA e 45% (11/24) daqueles que receberam ácido ascórbico como solução controle evoluíram para crises límbicas motoras e para o estado de mal epiléptico, após a administração de pilocarpina. A gravidade das crises durante o período agudo do modelo foi significativamente maior nos ratos epilépticos experimentais (56,52%) do que nos ratos controle (4,16%). No segundo experimento, não houve diferença significante entre os grupos quanto à frequência de crises na fase crônica do modelo. Nossos dados mostraram que a dopamina pode desempenhar um papel importante na gravidade das crises na fase aguda da pilo, o que parece ser exercido por sua ação inibitória da dopamina sobre a expressão motora das crises.

Abstract The dopamine content in cerebral structures has been related to neuronal excitability and several approaches have been used to study this phenomenon during seizure vulnerability period. In the present work, we describe the effects of dopamine depletion after the administration of 6-hidroxidopamine (6-OHDA) into the substantia nigra pars compacta of male rats submitted to the pilocarpine model of epilepsy. Susceptibility to pilocarpine-induced status epilepticus (SE), as well as spontaneous and recurrent seizures (SRSs) frequency during the chronic period of the model were determined. Since the hippocampus is one of main structures in the development of this experimental model of epilepsy, the dopamine levels in this region were also determined after drug administration. In the first experiment, 62% (15/24) of 6-OHDA pre-treated rats and 45% (11/24) of those receiving ascorbic acid as control solution progressed to motor limbic seizures evolving to SE, after the administration of pilocarpine. Severeness of seizures during the model´s the acute period, was significantly higher in epileptic experimental rats (56.52%), than in controls (4.16%). In the second experiment, the frequency of seizures in the model’s chronic phase did not significantly change between groups. Our data show that dopamine may play an important role on seizure severity in the pilo’s model acute period, which seems to be due to dopamine inhibitory action on motor expression of seizure.

Industrial waste products are of biotechnological interest due to their abundance and can be utilized as protein sources for the production of bioactive peptides through hydrolysis. The utilization of yeast residues from the brewing industry has been demonstrated to be a viable method for obtaining protein hydrolysates. The degree of hydrolysis and soluble total protein of the hydrolysates were evaluated for optimization purposes to achieve a product with a high proportion of soluble organic nitrogen, increased levels of bioactive peptides and amino acids. Both alkaline and enzymatic hydrolysis processes were tested. The hydrolyses were carried out with the aim of large-scale implementation. The treatments were effective in liberating intracellular content and producing biologically functional hydrolysates, with degree of hydrolysis values ranging from 28 to 63% and total protein from 2.95 to 8.28% at the optimum points confrmed by statistical analysis (analysis of variance (ANOVA)). The hydrolysate produced with Alcalase® exhibited the highest peptide intensity, while the hydrolysate produced with Flavourzyme® showed the highest concentration of free amino acids. The peptides were identifed by mass spectrometry and were found to have biological activities according to the Biopep database. This study presents a quick and economically feasible approach for the implementation of a pilot project for the reuse of this waste. nitrogen acids tested largescale large scale 2 63 295 95 2.9 828 8 8.28 ANOVA. ANOVA . (ANOVA)) Alcalase intensity Flavourzyme database 6 29 9 2. 82 8.2 (ANOVA) 8. (ANOVA

Resumo: No Extremo Sul baiano, Município de Ibirapuã, sob irrigação, avaliou-se o desempenho produtivo de 20 variedades de laranjeiras-doces, com distintas épocas dematuração,com foco principal nas laranjeiras ‘Pera’ (18 clones), ‘Valência’ (18 clones)e ‘Natal’ (cinco clones), totalizando 58 variedades copa. Os porta-enxertos utilizados foram atangerineira‘Sunki Tropical’ e os citrandarins ‘Indio’, ‘Riverside’ e ‘San Diego’. Cadacombinação copa/porta- enxerto, totalizando 232, foi representada por cinco plantas, observando o espaçamento de plantio de 6 m x 3 m. A implantação do experimento deu-se em 21 de abril de2015. As copas Pera Seleção CNPMF A15; ‘Pera C-21’; Pera Seleção CNPMF D3; ‘Pera D25’; ‘Pera CNPMF E-6’; Pera Seleção CNPMF CE-03; Pera Seleção Olímpia; ‘Pera Bianchi’;Pera Seleção Vacinada; Pera Seleção Ipeal-E3; Pera Seleção Ibotirama; ‘Valência CNPMFMontemorelos’;‘Natal CNPMF-112’; Seleção Berna; Seleção F-Menuda; ‘BRS Sincorá’; ‘Aquiri’;Seleção Early Oblong; Seleção Russas P.S.; ‘Seleta Itaboraí’; ‘Pineapple’ e ‘Westin’ forammais produtivas no porta-enxerto ‘Sunki Tropical’. As copas Pera seleção Ibotirama, Valência Seleção CNPMF 02, Valência Seleção CNPMF 03; ‘Valência CNPMF-27’; Valência SeleçãoCNPMF 36;Valência Seleção L. White; ‘Natal CNPMF 112’; Seleção Berna; ‘Aquiri’; SeleçãoEarly Oblong;‘Salustiana’; ‘Diva’ e ‘Hamlin 20’ foram mais produtivas no porta-enxerto ‘SanDiego’. As copas Pera Seleção CNPMF 02; Pera Seleção CNPMF C-32; ‘Pera CNPMF D-6’; ‘Pera D25’;Pera Seleção Olímpia; Valência Seleção CNPMF 01; Valência Seleção CNPMF 02;Valência Seleção CNPMF; ‘Valência CNPMF 27’; Valência Seleção CNPMF 36; Valência Seleção CNPMF-F11;Valência Seleção Chapman; ‘Valência CNPMF Montemorelos’; ‘Valência CNPMFTuxpan’;Natal Seleção CNPMF 02; Natal Seleção Ipeal; ‘Natal CNPMF 112’; SeleçãoF-Menuda; ‘Seleta Itaboraí’; ‘Salustiana’; ‘Pineapple’; ‘Hamlin 20’ e ‘MelRosa’ foram maisprodutivas no porta-enxerto ‘Riverside’. As copas Pera Seleção CNPMF A15; ‘Pera D25’; PeraSeleção Ipeal E3;Valência Seleção CNPMF 01; Valência Seleção CNPMF 02; Valência Seleção CNPMF;Valência Seleção CNPMF 36; Valência Seleção CNPMF-F11; Valência Seleção Delta; Valência Seleção Chapman; ‘Valência CNPMF Montemorelos’; Valência Seleção Registro; ‘Valência CNPMF Tuxpan’; Valência Seleção CNPMF 21; Natal Seleção CNPMF 01; ‘Natal CNPMF 112’;Seleção Berna; ‘Aquiri’; ‘Diva’ e ‘Hamlin 20’ foram mais produtivas no porta-enxerto‘Indio’. Resumo baiano Ibirapuã irrigação avaliouse avaliou se 2 laranjeirasdoces, laranjeirasdoces doces, doces laranjeiras-doces dematuraçãocom dematuração 18 (1 clones, clones , clones) clonese 5 copa portaenxertos porta enxertos atangerineiraSunki atangerineira Sunki Tropical ‘Indio, Indio ‘Indio ‘Indio’ ‘Riverside Riverside San Diego. Diego . Diego’ copa/porta copaporta enxerto 232 plantas deuse deu de2015 A15 C21 C C-21’ D3 D D25 D25’ E6 E E-6’ CE03 CE 03 CE-03 Olímpia BianchiPera Bianchi Vacinada IpealE3 IpealE E3 Ipeal-E3 Ibotirama CNPMFMontemorelos‘Natal CNPMFMontemorelosNatal CNPMFMontemorelos CNPMF112 112 CNPMF-112’ Berna FMenuda F Menuda F-Menuda BRS Sincorá Sincorá’ ‘AquiriSeleção AquiriSeleção ‘Aquiri Aquiri Oblong P.S. PS P S Seleta Itaboraí Itaboraí’ ‘Pineapple Pineapple ‘Westin Westin portaenxerto Tropical. 02 CNPMF27 27 CNPMF-27’ 36Valência 36 L White 112’ ‘Aquiri’ Oblong‘Salustiana OblongSalustiana ‘Salustiana Salustiana Oblong;‘Salustiana’ ‘Diva Diva Hamlin ‘SanDiego. SanDiego ‘SanDiego ‘SanDiego’ C32 32 C-32 D6 D-6’ D25Pera DPera 01 02Valência 27’ CNPMFF11Valência CNPMFFValência F11 Chapman Montemorelos Montemorelos’ CNPMFTuxpanNatal CNPMFTuxpan SeleçãoFMenuda SeleçãoF SeleçãoF-Menuda ‘Salustiana’ ‘MelRosa MelRosa ‘Riverside. E3Valência EValência CNPMFValência CNPMFF11 CNPMFF CNPMF-F11 Delta Registro Tuxpan Tuxpan’ 112Seleção portaenxerto‘Indio. portaenxertoIndio enxerto‘Indio porta-enxerto‘Indio’ 1 ( 23 de201 A1 C2 C-21 D2 E-6 CE0 0 CE-0 Ipeal-E CNPMF11 11 CNPMF-112 P.S CNPMF2 CNPMF-27 Oblong;‘Salustiana C3 C-3 D-6 F1 CNPMFF1 CNPMF-F1 portaenxerto‘Indio enxertoIndio porta-enxerto‘Indio de20 C-2 E- CE- CNPMF1 CNPMF-11 CNPMF-2 C- D- CNPMF-F de2 CNPMF-1 CNPMF-

Abstract: In the Extreme South of Bahia, in the Municipality of Ibirapuã, under irrigation, the productive performance of 20 varieties of sweet orange trees, with different maturation times, was evaluated, with the main focus on the orange trees ‘Pera’ (18 clones), ‘Valencia’ (18 clones) and ‘Natal’ (five clones), totaling 58 tops varieties. The rootstocks used were the mandarin tree ‘Sunki Tropical’ and the citrandarins ‘Indio’, ‘Riverside’ and ‘San Diego’. Each top/rootstock combination, totaling 232, was represented by five plants, observing a planting spacing of 6 m x 3 m. The experiment was implemented on April 21, 2015. The tops Pera Selection CNPMF A15; ‘Pera C-21’; Pera Selection CNPMF D3; ‘Pera D25’; ‘Pera CNPMF E-6’; Pera Selection CNPMF CE-03; Pera Selection Olímpia; ‘Pera Bianchi’; Pera Selection Vacinada; Pera Selection Ipeal-E3; Pera Selection Ibotirama; ‘Valencia CNPMF Montemorelos’; ‘Natal CNPMF-112’; Selection Berna, Selection F-Menuda; ‘BRS Sincorá’; ‘Aquiri’; Selection Early Oblong; Selection Russas P.S.; ‘Seleta Itaboraí’; ‘Pineapple’ and ‘Westin’ were more productive on the ‘Sunki Tropical’ rootstock. The tops Pera Selection Ibotirama; Valencia Selection CNPMF 02; Valencia Selection CNPMF 03; ‘Valencia CNPMF-27’; Valencia Selection CNPMF 36; Valencia Selection L. White; ‘Natal CNPMF 112’; Selection Berna; ‘Aquiri’; Selection Early Oblong; ‘Salustiana’; ‘Diva’ and ‘Hamlin 20’ were more productive on the ‘San Diego’ rootstock. The tops Pera Selection CNPMF 02; Pera Selection CNPMF C-32; ‘Pera CNPMF D-6’; ‘Pera D25’; Pera Selection Olímpia; Valencia Selection CNPMF 01; Valencia Selection CNPMF 02; Valencia Selection CNPMF; ‘Valencia CNPMF 27’; Valencia Selection CNPMF 36; Valencia Selection CNPMF-F11; Valencia Selection Chapman; ‘Valencia CNPMF Montemorelos’; ‘Valencia CNPMF Tuxpan’; Natal Selection CNPMF 02; Natal Selection Ipeal; ‘Natal CNPMF 112’; Selection F-Menuda, ‘Seleta Itaboraí’; ‘Salustiana’; ‘Pineapple’; ‘Hamlin 20’ and ‘MelRosa’ were more productive on the ‘Riverside’ rootstock. The tops Pera Selection CNPMF A15; ‘Pera D25’; Pera Selection Ipeal E3; Valencia Selection CNPMF 01; Valencia Selection CNPMF 02; Valencia Selection CNPMF; Valencia Selection CNPMF 36; Valencia Selection CNPMF-F11;Valencia Selection Delta; Valencia Selection Chapman; ‘Valencia CNPMF Montemorelos’;Valencia Selection Registro; ‘Valencia CNPMF Tuxpan’; Valencia Selection CNPMF21; Natal Selection CNPMF 01; ‘Natal CNPMF 112’; Selection Berna; ‘Aquiri’; ‘Diva’ and‘Hamlin 20’were more productive on the ‘Indio’ rootstock. Abstract Bahia Ibirapuã irrigation 2 times evaluated 18 (1 clones, clones , 5 Sunki Tropical ‘Indio, Indio ‘Indio ‘Riverside Riverside San Diego. Diego . toprootstock top rootstock combination 232 plants 21 2015 A15 A C21 C C-21’ D3 D D25 D25’ E6 E E-6’ CE03 CE 03 CE-03 Olímpia Bianchi Bianchi’ Vacinada IpealE3 IpealE E3 Ipeal-E3 Ibotirama Montemorelos Montemorelos’ CNPMF112 112 CNPMF-112’ Berna FMenuda F Menuda F-Menuda BRS Sincorá Sincorá’ ‘Aquiri Aquiri ‘Aquiri’ Oblong P.S. PS P S Seleta Itaboraí Itaboraí’ ‘Pineapple Pineapple ‘Westin Westin 02 CNPMF27 27 CNPMF-27’ 36 L White 112’ ‘Salustiana Salustiana ‘Salustiana’ ‘Diva Diva Hamlin C32 32 C-32 D6 D-6’ 01 27’ CNPMFF11 CNPMFF F11 CNPMF-F11 Chapman Tuxpan Tuxpan’ FMenuda, Menuda, ‘MelRosa MelRosa CNPMFF11Valencia CNPMFFValencia Delta MontemorelosValencia Registro CNPMF21 andHamlin 20were 1 ( 23 201 A1 C2 C-21 D2 E-6 CE0 0 CE-0 Ipeal-E CNPMF11 11 CNPMF-112 P.S CNPMF2 CNPMF-27 C3 C-3 D-6 CNPMFF1 F1 CNPMF-F1 C-2 E- CE- CNPMF1 CNPMF-11 CNPMF-2 C- D- CNPMF-F CNPMF-1 CNPMF-

Resumo O presente estudo buscou avaliar o potencial antimicrobiano e antiaderente do óleo essencial de Eucalipto radiata frente às cepas de veiculação alimentar de Escherichia coli. O estudo foi realizado usando a Concentração Inibitória Mínima (CIM) e Concentração Bactericida Mínima (CBM). Além disso, a técnica de difusão em disco foi usada para avaliar a associação do óleo essencial de Eucalipto radiata com antimicrobianos sintéticos. A Concentração Inibitória Mínima de Aderência (CIMA) também foi realizada. Os resultados revelaram que E. radiata apresentou atividade antimicrobiana contra as cepas de E. coli testadas, com valores de CIM variando entre 500 μg/mL a 1000 μg/mL e valores de CBM variando entre 500 μg/mL a 1,024 μg/mL. Quanto às associações, observou-se que o óleo de E. radiata exibiu efeito sinérgico para alguns antibióticos, sobretudo a Ceftriaxona, apresentando maior interferência do óleo essencial. Além disso, foi eficaz na inibição a aderência das cepas bacterianas de E. coli, mostrando um efeito antibiofilme mais significativo que o agente antibacteriano digluconato de clorexidina a 0.12%. Em resumo, o óleo essencial de E. radiata apresentou potencial antimicrobiano contra cepas de E. coli de origem alimentar, podendo assim, mediante estudos aprofundados, ser utilizado isoladamente ou em associação com antimicrobianos sintéticos para combater infecções causadas por esse patógeno. (CIM CBM. . (CBM) disso CIMA (CIMA realizada E testadas 50 μgmL μg mL 100 1024 1 024 1,02 associações observouse observou se antibióticos Ceftriaxona 012 0 12 0.12% resumo assim aprofundados patógeno (CBM 5 10 102 02 1,0 01 0.12 1, 0.1 0.

Abstract The present study sought to evaluate the antimicrobial and anti-adherent potential of Eucalyptus radiata essential oil against food-borne strains of Escherichia coli. The study was performed using the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC). In addition, the disk diffusion technique was used to evaluate the association of Eucalyptus radiata essential oil with synthetic antimicrobials. The Minimum Inhibitory Adherence Concentration (MIC) was also performed. The results revealed that E. radiata showed antimicrobial activity against the E. coli strains tested, with MIC values ranging from 500 μg/mL to 1000 μg/mL and MBC values ranging from 500 μg/mL to 1,024 μg/mL. As for the associations, it was observed that E. radiata oil exhibited a synergistic effect for some antibiotics, especially Ceftriaxone, with greater interference from the essential oil. Furthermore, it was effective in inhibiting the adherence of bacterial strains of E. coli, showing a more significant antibiofilm effect than the antibacterial agent 0.12% chlorhexidine digluconate. In summary, the essential oil of E. radiata showed antimicrobial potential against strains of E. coli of food origin, and can therefore, through in-depth studies, be used alone or in association with synthetic antimicrobials to combat infections caused by this pathogen. antiadherent anti adherent foodborne borne (MIC MBC. . (MBC) addition E tested 50 μgmL μg mL 100 1024 1 024 1,02 associations antibiotics Ceftriaxone Furthermore 012 0 12 0.12 digluconate summary origin therefore indepth depth studies pathogen (MBC 5 10 102 02 1,0 01 0.1 1, 0.

A new phenylpropanoid derivative, named euploic acid (1), was isolated from Euploca procumbens (Mill.) Diane & Hilger, Boraginaceae, along with lithospermic acid B (2), lithospermic acid (3), 9’’-methyl lithospermate (4), and luteolin-7-O-glucoside (5). Compound structures were determined by mass spectrometry (MS), nuclear magnetic resonance (NMR) analysis and comparison with published data. Absolute configuration of 1 was established via electronic circular dichroism (ECD). Anti-inflammatory potential of euploic acid (1) was assessed by measuring its inhibition of cytokine and nitric oxide production in stimulated J774 macrophages. Compound 1 significantly reduced pro-inflammatory mediator production and release by stimulated macrophages at non-cytotoxic concentrations, comparable to the efficacy of dexamethasone. Our findings demonstrate the potential of euploic acid (1) as an effective anti-inflammatory agent. This study contributes to the structural elucidation of a new phenylpropanoid derivative and highlights its promising anti-inflammatory activity in the treatment of inflammatory disorders. 1, , Mill. Mill (Mill. Hilger Boraginaceae 2, 2 (2) 3, 3 (3) 9methyl methyl 9 4, 4 (4) luteolin7Oglucoside luteolinOglucoside luteolin 7 O glucoside 5. 5 . (5) MS, MS (MS) NMR (NMR data ECD. ECD (ECD) Antiinflammatory Anti (1 J J77 proinflammatory pro noncytotoxic non cytotoxic concentrations dexamethasone antiinflammatory anti agent disorders (Mill (2 (3 (4 Oglucoside (5 (MS (ECD ( J7

Abstract Sarcopenia is a pathology resulting from a progressive and severe loss of muscle mass, strength, and function in the course of aging, which has deleterious consequences on quality of life. Among the most widespread studies on the issue are those focused on the effect of different types of physical exercise on patients with sarcopenia. This randomized controlled study aimed to compare the effects of a whole-body vibration exercise (WBV) session on the inflammatory parameters of non-sarcopenic (NSG, n=22) and sarcopenic elderly (SG, n=22). NSG and SG participants were randomly divided into two protocols: intervention (squat with WBV) and control (squat without WBV). After a one-week washout period, participants switched protocols, so that everyone performed both protocols. Body composition was assessed by dual-energy radiological absorptiometry (DXA) and function through the six-minute walk test (6MWD) and Short Physical Performance Battery (SPPB). Plasma soluble tumor necrosis factor receptors (sTNFR) were determined by enzyme-linked immunosorbent assay (ELISA) and measured before and immediately after each protocol. After exercise with WBV, there was an increase in sTNFR2 levels in the NSG (P<0.01; d=-0.69 (-1.30; -0.08) and SG (P<0.01, d=-0.95 (-1.57; -0.32) groups. In conclusion, an acute session of WBV influenced sTNFr2 levels, with sarcopenic individuals showing a greater effect. This suggested that WBV had a more pronounced impact on sTNFr2 in those with loss of muscle strength and/or physical performance. Additionally, WBV is gaining recognition as an efficient strategy for those with persistent health issues. mass aging life sarcopenia wholebody whole body (WBV nonsarcopenic non NSG, (NSG n=22 n22 n 22 SG, (SG n=22. . protocols squat WBV. oneweek one week period dualenergy dual energy DXA (DXA sixminute six minute 6MWD MWD (6MWD SPPB. SPPB (SPPB) sTNFR (sTNFR enzymelinked enzyme linked ELISA (ELISA protocol P<0.01 P001 P 0 01 (P<0.01 d=0.69 d069 d d= 0.69 69 d=-0.6 1.30 130 1 30 (-1.30 0.08 008 08 -0.08 P<0.01, d=0.95 d095 0.95 95 d=-0.9 1.57 157 57 (-1.57 0.32 032 32 -0.32 groups conclusion sTNFr andor or performance Additionally issues n=2 n2 2 (SPPB P<0.0 P00 (P<0.0 d=0.6 d06 069 0.6 6 d=-0. 1.3 13 3 (-1.3 0.0 00 -0.0 d=0.9 d09 095 0.9 9 1.5 15 5 (-1.5 0.3 03 -0.3 n= P<0. P0 (P<0. d=0. d0 06 0. d=-0 1. (-1. -0. 09 P<0 (P<0 d=0 d=- (-1 -0 P< (P< (- - (P (

Abstract Natural products have been studied as potential anthelmintic drugs. This study aimed to evaluate the effects of the polypeptides/proteins exuded from Mimosa caesalpiniifolia mature seeds on the model nematode Caenorhabditis elegans. After seed immersion for 24 h at 25 ºC in sodium phosphate buffer, pH 7.0, containing 0.15 M NaCl and 1.0 M MgSO4, the constituent polypeptides/proteins were fractionated using ammonium sulfate precipitation followed by ultrafiltration and their effects on C. elegans motility were assessed. Moreover, we evaluated by Atomic Force Microscopy (AFM) whether exposition of the nematode to the polypeptide fraction McSEX10-2 could cause any alteration on the animal’s external surface (cuticle). The whole seed exudates (SEX) from the M. caesalpiniifolia mature seeds (named McSEX) and its polypeptide/protein derived fractions, termed McSEX90%/80°C, McSEX30-10, and McSEX10-2, inhibited the C. elegans motility with an IC50 of 15.18, 12.59, 9.74 and 1.34 μg mL-1.The McSEX10-2 fraction, which encompassed the polypeptides with the molecular mass varying from 10 kDa to 2 kDa, was the most effective. In addition, McSEX10-2 altered the ultrastructure and the mechanical properties of the C. elegans cuticle. McSEX10-2, in particular, can be used as a model to bioinspire structure-based design of new peptides that could control, alone or in combination with other drugs, economically important parasitic nematodes. drugs polypeptidesproteins proteins buffer 70 7 0 7.0 015 15 0.1 1 1. MgSO4 MgSO C assessed Moreover AFM (AFM McSEX102 McSEX McSEX10 McSEX10- animals animal s cuticle . (cuticle) SEX (SEX named polypeptideprotein protein fractions McSEX9080C McSEXC McSEX90 80 McSEX90%/80°C McSEX3010, McSEX3010 McSEX30 10, McSEX30-10 McSEX102, 2, IC IC5 1518 18 15.18 1259 12 59 12.59 974 9 74 9.7 134 34 1.3 mL1.The mL1The mLThe mL 1.The effective addition particular structurebased structure based control nematodes 7. 01 0. McSEX1 (cuticle McSEX9 8 McSEX301 McSEX3 McSEX30-1 151 15.1 125 5 12.5 97 9. 13 3 mL1 1The McSEX30- 15. 12.

Resumo O diagnóstico rápido da tuberculose bovina (TB), permite a atuação de instituições oficiais de vigilância em áreas que possam constituir possíveis focos de propagação da doença. Essa pesquisa identificou o agente Mycobacterium bovis através do PCR multiplex das colônias isoladas. Através do serviço de inspeção oficial (Federal e Estadual), foram coletadas amostras sugestivas de TB durante a inspeção post-mortem de carcaças bovinas em matadouros-frigoríficos e estas foram encaminhadas ao laboratório para os diagnósticos complementares. As amostras foram comprovadas por meio de cultura bacteriológica e coloração Ziehl-Neelsen. Nos isolados caracterizados por Bacilos ÁcidoÁlcool Resistente (BAAR), aplicou-se a técnica de PCR multiplex visando a confirmação do agente Mycobacterium bovis. Foram analisadas 991.316 carcaças de bovinos. Entre estas, 32 apresentaram amostras sugestivas de TB que foram coletadas, provenientes de animais de 30 municípios. Entre as amostras coletadas, 28,1% (9/32) apresentaram crescimento em meios de cultura bacteriológicos com BAAR. Os BAAR isolados submetidos à PCR multiplex confirmaram a presença de M. bovis. A técnica de PCR multiplex associada ao exame bacteriológico e aplicada aos achados post-mortem permitiu o diagnóstico de M. bovis nos municípios amostrados do estado da Bahia. TB, , (TB) doença isoladas Federal Estadual, Estadual Estadual) postmortem post mortem matadourosfrigoríficos matadouros frigoríficos complementares ZiehlNeelsen. ZiehlNeelsen Ziehl Neelsen. Neelsen Ziehl-Neelsen BAAR, (BAAR) aplicouse aplicou se 991316 991 316 991.31 bovinos 3 281 28 1 28,1 9/32 932 9 (9/32 M Bahia (TB (BAAR 99131 99 31 991.3 2 28, 9/3 93 (9/3 9913 991. 9/ (9/ (9 (

Abstract The rapid diagnosis of bovine tuberculosis (BT) allows official surveillance institutions to act in areas that could be potential hotspots for the spread of the disease. The study aimed to identify the Mycobacterium bovis agent by multiplex PCR of isolated colonies. Through the official inspection service (Federal/State), samples suggestive of BT were collected during the post-mortem inspection of bovine carcasses in slaughterhouses and sent to the laboratory for complementary diagnostics. The samples were analyzed using bacteriological culture and Ziehl-Neelsen staining. In isolates characterized as Acid-Alcohol Resistant Bacilli (BAAR), the multiplex PCR technique confirmed the agent Mycobacterium bovis. Nine hundred ninety-one thousand three hundred sixteen cattle carcasses were inspected, collecting 32 BT-suggestive samples in from animalsfrom 30 municipalities. Of these, 28.1% (9/32) showed growth on bacteriological culture media with BAAR. The BAAR isolates submitted to multiplex PCR confirmed the presence of M. bovis. The multiplex PCR technique associated with bacteriological examination and applied to post-mortem findings enabled the diagnosis of M. bovis in the municipalities sampled in the Bahia state. (BT disease colonies Federal/State, FederalState Federal/State , Federal State (Federal/State) postmortem post mortem diagnostics ZiehlNeelsen Ziehl Neelsen staining AcidAlcohol Acid Alcohol BAAR, (BAAR) ninetyone ninety one inspected 3 BTsuggestive these 281 28 1 28.1 9/32 932 9 (9/32 M state (Federal/State (BAAR 2 28. 9/3 93 (9/3 9/ (9/ (9 (

Abstract The ecology of movement is an expanding area, marked by the diversity of analytical methods and protocols, which enables this integrative reading. We investigated movement ecology aspects of Coleodactylus meridionalis in southern Bahia, northeastern Brazil, using fluorescent powder with mineral oil to track individuals. We monitored 69 individuals of C. meridionalis that walked an average distance of 148 cm in 2h. We identified this movement as foraging due to the orientation of the step sequence and microenvironments used. We find no significant differences between walking distance and weight. However, we found a decrease in activity over the follow-up period. Most of the lizard’s movements were directed north, while south, east, and west were followed equally. The individuals stayed predominantly on the ground (leaf litter), but it was possible to observe the use of other surfaces, such as trunks and burrows on the ground. Therefore, we studied the movement in three dimensions (ground height, distance traveled, and orientation of steps). We observed the lizard’s foraging, one of the most common and least investigated movements in small lizards like C. meridionalis. This involves not only the species’ activity schedule but other intrinsic and extrinsic factors that shape the movement decisions of individuals. area protocols reading Bahia Brazil 6 C 14 2h h used weight However followup follow up period lizard s north south east equally leaf litter, litter , litter) surfaces Therefore height traveled steps. steps . steps) species 1