ABSTRACT Planting is considered one of the most essential steps in coffee growing. Lack of uniformity in planting may compromise future operations. Therefore, verifying planting operations quality is fundamental to optimizing production processes and reducing costs. This study aimed to investigate planting techniques through Statistical Process Control (SPC) and aerial images. Carried out in two areas, managed manually and semi-mechanized in the Bom Jardim Farm (MG - Brazil). Data were collected through Remotely Piloted Aircraft (RPA). Quality control charts and density maps were used to identify variations in distribution and spacing between plants and planting rows. It was found that the planting carried out manually was 4.7% wider than projected due to spacing reduction from 0.5 m to 0.48 m. The semi-mechanized system displayed a deficit of 7% compared to the projected planting system, using 0.55 m between plants. The density map showed the most significant planting alignment variations. Despite displaying lower results than the manual system, the semi-mechanized system improvements are valid for their minimal average variations. Thus, correcting points found outside the limits can increase the efficiency of semi-mechanized planting. growing Therefore costs SPC (SPC images areas semimechanized semi mechanized MG Brazil. Brazil . Brazil) RPA. RPA (RPA) rows 47 4 7 4.7 05 0 5 0. 048 48 0.4 055 55 Thus (RPA 4. 04

ABSTRACT BACKGROUND: Genitourinary health significantly affects the quality of life of men, particularly those in middle age. Recent studies have shown that more than half of the men aged over 40 years experience some degree of low urinary tract symptoms (LUTS) or erectile dysfunction (ED). OBJECTIVE: To assess the prevalence of ED and LUTS in middle-aged men and correlate this with quality of life data. DESIGN AND SETTING: A cross-sectional study was conducted in a municipality in the countryside of São Paulo. METHODS: A trained team collected data between July 2021 and August 2022 through face-to-face interviews using a characterization instrument, International Prostate Symptom Score (IPSS), International Index of Erectile Function-6 (IIEF-6), and World Health Organization Quality-of-Life Scale. RESULTS: The study included 375 male participants with a median age of 53 years (interquartile range [IQR] 38.5-66). The IIEF-6 showed the presence of some degree of ED in 51.1% (n = 188) of patients, with a median score of 25 (IQR 21-29). The IPSS revealed that 35.2% (n = 132) of the patients had some degree of LUTS, with a median score of 5 (IQR 2-11). The urological questionnaires had a direct proportional correlation with age (P < 0.001) and significant differences between the medians of different marital statuses (P < 0.001). The presence or severity of these disorders was inversely correlated with the individuals’ quality of life (P < 0.001). CONCLUSIONS: ED and LUTS significantly correlated with the quality of life, marital status, and age in men. BACKGROUND 4 (LUTS ED. . (ED) OBJECTIVE middleaged SETTING crosssectional cross sectional Paulo METHODS 202 facetoface face to instrument IPSS, , (IPSS) Function6 Function 6 Function- IIEF6, IIEF6 IIEF (IIEF-6) QualityofLife Quality Life Scale RESULTS 37 interquartile IQR [IQR 38.566. 38566 38.5 66 38 38.5-66) IIEF- 511 51 1 51.1 n 188 2 2129. 2129 21 29 21-29) 352 35 35.2 132 211. 211 11 2-11) P 0.001 0001 0 001 0.001. individuals CONCLUSIONS status (ED 20 (IPSS (IIEF-6 3 566 38.566 3856 385 38. 38.5-66 51. 18 212 21-29 35. 13 2-11 0.00 000 00 (IIEF- 56 38.56 38.5-6 21-2 2-1 0.0 (IIEF 38.5- 21- 2- 0.

Resumen: Este estudio tuvo como objetivo estimar la prevalencia del COVID-19 e identificar los factores sociales y las estrategias preventivas asociadas a esta enfermedad en personas en situación de vulnerabilidad socioeconómica (recolectores de basura, inmigrantes/refugiados y personas sin hogar) en Goiânia, Estado de Goiás, en la región central de Brasil. Se trata de un estudio transversal realizado de julio a octubre de 2020. Las personas que tuvieron una prueba de anticuerpos anti-SARS-CoV-2 positiva y/o una prueba de ARN para SARS-CoV-2 se consideraron positivas para el COVID-19. Se realizaron análisis de regresión univariante y múltiple para identificar las variables asociadas al COVID-19. De los 594 participantes, el 47,3% eran recicladores; el 29,6% inmigrantes/refugiados; y el 23,1% eran personas sin hogar. La positividad fue del 14,1% para el ARN del SARS-CoV-2; del 30,8% para el total de anti-SARS-CoV-2; y del 39,4% para al menos un marcador del COVID-19. De los 541 participantes, ser inmigrante/refugiado, no usar mascarilla quirúrgica y tener tres o más personas que comparten la misma habitación fueron los factores asociados con la infección por SARS-CoV-2, mientras que confiar en la información sobre la pandemia, especialmente transmitidas por los noticiarios, fue un indicador de protección contra esta enfermedad. Este estudio reveló las desigualdades étnicas y socioeconómicas en la prevalencia del COVID-19 entre las personas de bajos ingresos en Brasil. Además, se detectó una alta prevalencia del COVID-19 en los tres grupos. El desarrollo de nuevas estrategias para combatir y prevenir las enfermedades transmisibles que afectan a esta población es fundamental para mitigar las pandemias futuras y actuales.

Abstract: This study aimed to estimate the prevalence and identify social factors and preventive strategies associated with the coronavirus disease 2019 (COVID-19) in socio and economically vulnerable people (recyclable waste pikers, immigrants/refugees, and homeless people) in Goiânia, Goiás State, Central-Western Brazil. A cross-sectional study was conducted from July 2020 to October 2020. COVID-19 positivity was defined as a positive total anti-SARS-COV-2 antibody test and/or RNA test for SARS-COV-2. Univariable and multiple regression analyses were performed to identify the variables associated with COVID-19. Of the 594 participants, 47.3% were recyclable waste pickers, 29.6% were immigrants/refugees, and 23.1% were homeless people. The positivity for SARS-CoV-2 RNA was 14.1%, whereas for anti-SARS-CoV-2 a total of 30.8% were positive, and 39.4% were positive for at least one COVID-19 marker. Among the 541 individuals, being immigrants/refugees, not wearing a surgical mask, and having three or more people sleeping in the same room were associated with SARS-CoV-2 infection, while using TV news as the main source of information about the pandemic was a protective predictor of COVID-19. This study revealed ethnic and socioeconomic inequalities in the prevalence of COVID-19 among impoverished people in Brazil. Additionally, a high prevalence of COVID-19 was detected in all three groups. Developing new strategies to combat and prevent communicable diseases affecting this population is essential for mitigating future and ongoing pandemics.

Resumo: Este estudo teve como objetivo estimar a prevalência da COVID-19 e identificar fatores sociais e estratégias preventivas associadas a essa doença em pessoas em situação de vulnerabilidade socioeconômica (catadores de lixo, imigrantes/refugiados e moradores de rua) em Goiânia, Estado de Goiás, na região central do Brasil. Trata-se de um estudo transversal realizado de julho a outubro de 2020. Foram considerados positivos para a covid-19 indivíduos que apresentaram um teste de anticorpos anti-SARS-COV-2 e/ou teste de RNA para SARS-COV-2 com resultado positivo. Foram realizadas análises de regressão univariável e múltipla para identificar as variáveis associadas à COVID-19. Dos 594 participantes, 47,3% eram catadores de lixo reciclável, 29,6% eram imigrantes/refugiados e 23,1% eram moradores de rua. A positividade foi de 14,1% para o RNA do SARS-CoV-2, 30,8% para o total de anti-SARS-CoV-2 e 39,4% para pelo menos um marcador da COVID-19. Entre os 541 indivíduos, ser imigrante/refugiado, não usar máscara cirúrgica e ter três ou mais pessoas dormindo no mesmo quarto foram fatores associados à infecção por SARS-CoV-2, enquanto confiar em informações sobre a pandemia, principalmente aquelas transmitidas por noticiários de TV, foi um indicador de proteção contra a COVID-19. Este estudo revelou desigualdades étnicas e socioeconômicas na prevalência da COVID-19 entre pessoas de baixa renda no Brasil. Além disso, uma alta prevalência de COVID-19 foi detectada nos três grupos. O desenvolvimento de novas estratégias para combater e prevenir doenças transmissíveis que afetam essa população é essencial para mitigar pandemias futuras e atuais.

RESUMO O conhecimento das propriedades físicas e mecânicas da madeira é crucial para a segurança e eficácia do dimensionamento das estruturas. Além de favorecer a diversificação e abastecimento do mercado madeireiro, a determinação de tais propriedades, para diferentes espécies nativas, pode contribuir para a redução da exploração concentrada de madeiras tropicais. Entre as madeiras disponíveis para a construção civil na Região Tocantina do Maranhão encontra-se o tuturubá (Pouteria oblanceolata Pires). Neste contexto, este estudo teve como objetivo determinar as propriedades físicas e mecânicas da madeira de tuturubá, visando o seu uso em estruturas de edificações. Foram determinados: o teor de umidade, a densidade aparente, as resistências à compressão paralela e perpendicular às fibras, a resistência ao cisalhamento paralelo às fibras, a resistência à flexão e os módulos de elasticidade à compressão paralela às fibras e à flexão. Obteve-se um teor de umidade médio de 13,88%, uma densidade aparente média de 992,04 kg.m-3, o valor médio da resistência à compressão paralela às fibras igual a 60,03 MPa e o valor característico de 56,86 MPa, sendo o módulo de elasticidade à compressão paralela às fibras de 22761 MPa. A resistência média à compressão perpendicular às fibras foi de 10,74 MPa e a resistência média ao cisalhamento paralelo às fibras foi igual a 10,47 MPa. A resistência à flexão média foi de 154,82 MPa e o valor característico igual a 128,37 MPa, enquanto o valor médio do módulo de elasticidade à flexão foi de 21026 MPa. Os resultados permitiram classificar a madeira de tuturubá como pertencente à classe de resistência mecânica D50. Comparativamente com outras espécies disponíveis no mercado madeireiro, a madeira de tuturubá apresentou propriedades mecânicas superiores às madeiras de pequiá (Caryocar villosum) e tauari (Couratari oblongifolia). Esta caracterização físico-mecânica evidencia o potencial da madeira de tuturubá para utilização em estruturas de edificações. madeireiro nativas tropicais encontrase encontra se Pouteria Pires. Pires . Pires) contexto edificações determinados Obtevese Obteve 1388 13 88 13,88% 99204 992 04 992,0 kg.m3, kgm3 kgm kg.m 3, kg m 3 kg.m-3 6003 60 03 60,0 5686 56 86 56,8 2276 1074 10 74 10,7 1047 47 10,4 15482 154 82 154,8 12837 128 37 128,3 2102 D50 D Caryocar villosum Couratari oblongifolia. oblongifolia oblongifolia) físicomecânica físico 138 1 8 13,88 9920 99 0 992, m3 kg.m3 kg.m- 600 6 60, 568 5 56, 227 107 7 10, 104 4 1548 15 154, 1283 12 128, 210 D5 13,8 9 22 21 13, 2

ABSTRACT Knowledge of the physical and mechanical properties of wood is crucial for the safety and efficiency of structural design. In addition to promoting diversification and supply in the timber market, determining these properties for different types of native woods can contribute to reduce the concentrated overexploitation of tropical woods. Among the woods available for construction purposes in the Tocantina region of Maranhão State is tuturubá (Pouteria oblanceolata Pires). In this context, the present study aimed to evaluate the physical and mechanical properties of tuturubá wood for its use in building structures. The characterizations carried out were: moisture content, apparent density, compressive strength parallel and perpendicular to the grain, shear strength parallel to the grain, flexural strength, modulus of elasticity in compression parallel to the grain and three-point bending test. For the average moisture content, a percentage value of 13.88% was checked, and for the average apparent density, a value of 992.04 kg.m-3 was obtained. For the compressive strength parallel to the grain, an average value of 60.03 MPa was determined, while the characteristic value was 56.86 MPa. The compressive modulus of elasticity parallel to the grain determined was equal to 22761 MPa. The average values for compressive strength perpendicular to the grain and shear strength parallel to the grain were 10.74 MPa and 10.47 MPa, respectively. For flexural strength, average and characteristic values were 154.82 and 128.37 MPa, respectively. For the flexural modulus of elasticity, an average value of 21026 MPa was determined. The results allowed tuturubá wood to be classified in mechanical resistance class D50. Compared to other commercially available wood species, tuturubá has better mechanical properties than pequiá (Cary-ocar villosum) and tauari (Couratari oblongifolia) woods. This physical-mechanical characterization high-lights the potential of tuturubá wood for use in building structures. design market Pouteria Pires. Pires . Pires) context structures content density threepoint three point test 1388 13 88 13.88 checked 99204 992 04 992.0 kg.m3 kgm3 kgm kg.m 3 kg m kg.m- obtained 6003 60 03 60.0 5686 56 86 56.8 2276 1074 10 74 10.7 1047 47 10.4 respectively 15482 154 82 154.8 12837 128 37 128.3 2102 D50 D species Caryocar Cary ocar villosum Couratari oblongifolia physicalmechanical highlights high lights 138 1 8 13.8 9920 99 0 992. m3 600 6 60. 568 5 56. 227 107 7 10. 104 4 1548 15 154. 1283 12 128. 210 D5 13. 9 22 21 2

Resumo Este estudo teve como objetivo examinar o efeito da idade relativa na potência muscular em crianças e adolescentes brasileiros. A amostra foi composta por 87.766 meninas e meninos com idades entre 7 e 16 anos. Os participantes foram agrupados em quatro subgrupos etários dentro de cada idade cronológica tendo como base as datas de nascimento e da coleta dos dados. A potência muscular dos membros superiores e inferiores foi avaliada por meio dos testes de arremesso de medicine ball de 2 kg, e salto horizontal, respectivamente. A distribuição normativa (fraco; razoável; bom; muito bom; excelente) dos dois testes de potência foi utilizada para a classificação. Os resultados mostraram associação significativa entre os subgrupos etários e a classificação normativa da potência muscular. Entre os meninos, houve aumento na frequência de indivíduos classificados como “muito bom/excelente” à medida que a idade relativa aumentava. Uma tendência semelhante foi observada entre as meninas. Os achados forneceram evidências do efeito de idade relativa na potência muscular de crianças e adolescentes brasileiros. Esses resultados têm implicações práticas na organização de atividades físicas estruturadas e esportes em diferentes contextos, pois indivíduos nascidos mais cedo podem ter vantagem no desempenho em tarefas que exijam potência muscular. Mais pesquisas são necessárias para explorar os mecanismos subjacentes e intervenções que possam mitigar o efeito de idade relativa e promover oportunidades iguais para todas as crianças e adolescentes nas atividades físicas e esportes. brasileiros 87766 87 766 87.76 1 anos dados kg horizontal respectivamente fraco (fraco razoável bom excelente bom/excelente bomexcelente aumentava contextos 8776 8 76 87.7 877 87.

Abstract This study aimed to examine the relative age effect on muscle power in a large sample of Brazilian children and adolescents. The sample consisted of 87,766 girls and boys aged 7-16 years, data collected in repeated cross-sectional surveillance carried out since 1999. The participants were grouped into four age subgroups within each chronological age based on birthdates. Upper and lower limbs muscle power were assessed using the 2-Kg medicine ball throw and horizontal jump tests, respectively. The normative distribution of physical fitness components was used for classification. The results showed a significant association between relative age and normative classification of muscle power. Among boys, there was a sequential increase in the frequency of individuals classified as “very good/excellent” as the relative age increased. A similar trend was observed among girls. The findings provided evidence of a relative age effect on muscle power in Brazilian children and adolescents. These findings have implications for the organization of structured physical activities and sports, as early-born individuals may have an advantage in performance. Further research is needed to explore the underlying mechanisms and potential interventions to mitigate the relative age effect and promote equal opportunities for all youth in physical activities and sports. adolescents 87766 87 766 87,76 716 7 16 7-1 years crosssectional cross sectional 1999 birthdates 2Kg Kg 2 tests respectively very good/excellent goodexcellent good excellent increased sports earlyborn early born performance 8776 8 76 87,7 71 1 7- 199 877 87, 19

Resumo Alchornea sidifolia é uma espécie arbórea utilizada na regeneração de ambientes florestais degradados e que cresce tanto em solos não alagados quanto alagados. Nós comparamos a anatomia da madeira de árvores crescendo sob ambas as condições em remanescentes de Mata Atlântica no estado do Rio de Janeiro, Brasil, para entender aspectos intraespecíficos da adaptação de espécies lenhosas tropicais a essas condições. As árvores que se desenvolvem em solos permanentemente inundados apresentaram elementos de vasos mais largos, curtos e menos frequentes; fibrotraqueídes mais largas, com maior proporção do tipo gelatinoso; e menor frequência de parênquima radial, mas com séries de parênquima axial mais longas. Esses resultados indicam que as árvores de A. sidifolia crescendo em solo permanentemente inundado não apresentam déficit hídrico e que a espécie é capaz de maximizar o uso da água nesta condição de crescimento. Essa conclusão pode estar diretamente relacionada à maior proporção de fibrotraqueídes gelatinosos em árvores de ambientes alagados e contraria o que se esperava para a anatomia do lenho de árvores sob seca fisiológica. Janeiro Brasil largos frequentes largas gelatinoso radial longas A crescimento fisiológica

Abstract Alchornea sidifolia is a tree species used in the regeneration of degraded forest environments and which grows in both non-flooded and flooded soils. We compared the wood anatomy of trees growing under both conditions in Atlantic Forest remnants in the state of Rio de Janeiro, Brazil, to understand intraspecific aspects of the adaptation of tropical woody species to these conditions. Trees from permanently flooded soils showed wider, shorter, and less frequent vessel elements; wider fibretracheids, with a greater proportion of the gelatinous type; and a lower frequency of radial parenchyma, but with longer strands of axial parenchyma. These results indicate that A. sidifolia trees growing in permanently flooded sites do not show water deficit and that the species is capable of maximizing water use in this growth condition. This conclusion may be directly related to the greater proportion of gelatinous fibretracheids in flooded trees and is contrary to what was expected for wood anatomy of trees under physiological drought. nonflooded non Janeiro Brazil shorter elements type parenchyma A condition drought

Abstract Background In view of the high prevalence of hypertension and the importance of adequate drug therapy in the prevention of complications, it is necessary to know the adherence to drug treatment in this population. Objective To verify adherence to antihypertensive drug treatment in Brazilian patients with hypertension using the Morisky-Green Test (MGT), relating it with demographic data. Methods Prospective, observational, multicenter, national registry study, with 2,578 hypertensive patients participating in study I, the Brazilian Cardiovascular Registry of Arterial Hypertension (I-RBH), recruited in the five regions of Brazil. The analyses carried out on the data were descriptive statistics, qui-square tests, ANOVA, and logistic regression, adopting 5% as the significance level for the tests. Results The research shows that 56.13% of patients in the sample were female; 56.71% were elderly (≥ 65 years); 55.86% were White; 52.37% were from the Southeast Region; and 59.74% were non-adherent. Logistic regression showed an independent relationship between patients’ age, ethnicity, and region with medication adherence. Conclusion Adherence to treatment is the key to reducing high rates of cardiovascular complications. The study brings a successful outcome in the relationship between the factors ethnicity, age, and region of patients with hypertension and medication adherence. To this end, it is necessary to understand these factors, considering systematic evaluation in the care of patients with hypertension and other chronic non-communicable diseases. This study is a significant contribution to multidisciplinary teams, as it highlights which risk factors interfere with medication adherence, incorporating better strategies in health education. complications population MoriskyGreen Morisky Green MGT, MGT , (MGT) Prospective observational multicenter 2578 2 578 2,57 I IRBH, IRBH RBH (I-RBH) Brazil statistics quisquare qui square tests ANOVA 5 5613 56 13 56.13 female 5671 71 56.71 ≥ ( 6 years years) 5586 55 86 55.86 White 5237 52 37 52.37 Region 5974 59 74 59.74 nonadherent. nonadherent non adherent. adherent non-adherent age ethnicity end noncommunicable communicable diseases teams education (MGT 257 57 2,5 (I-RBH 561 1 56.1 567 7 56.7 558 8 55.8 523 3 52.3 597 59.7 25 2, 56. 55. 52. 59.

ABSTRACT Regosols (Neossolos) are soils with use limitations mainly related to effective depth, abundant presence of rock and saprolite fragments, and frequently high slope gradients; besides that, they represent a new agricultural frontier for grain production in southern Brazil. This study evaluated soil saturated hydraulic conductivity (Ksat) and water retention and availability in Regosols and saprolites derived from volcanic rocks in southern Brazil and the relationship of these variables with porosity in saprolithic horizons characterized by mineralogical weathering. The study was carried out on eight profiles derived from basic and acidic volcanic rocks of the Serra Geral Formation. We evaluated soil morphology, granulometry, porosity, bulk density (BD), Ksat, water retention, electronic/ optical microscopy and chemical composition of parent materials, and soil mineralogy. Soil Ksat ranged from 0.0 to 6.40 cm h-1 in the evaluated horizons, without significant difference between the A and Cr horizons. Seven soil profiles showed BD equal to or less than 1.28 Mg m-3 for the Cr samples. Total porosity in the Cr horizons was above 0.5 m3 m-3 and not significantly different from A horizons. In five of the eight soil profiles, one or more Cr horizons presented greater available water than A horizons. Electronic and optical microscopy evidenced abundant cracks and mineralogical weathering in the rock samples. X-rays diffraction data also indicated advanced degree of weathering of Cr horizons, evidencing abundant formation of pores in the saprolite. and justifying the high-water retention in Regosols profiles in southern Brazil. Neossolos (Neossolos depth fragments gradients that (Ksat Formation morphology granulometry BD, , (BD) electronic materials mineralogy 00 0 0. 640 6 40 6.4 h1 h 1 h- 128 28 1.2 m 3 m- samples 05 5 Xrays X rays highwater (BD 64 4 6. 12 2 1.

Resumo Introdução A degradação não-cariosa da matriz dentinária é um processo natural ao longo da vida; no entanto, quando associada à presença de substâncias ácidas excessivas, leva a lesões nas estruturas dentárias. Objetivo Este estudo desenvolveu uma solução experimental baseada em polifenóis antioxidantes da semente do maracujá amarelo (Passiflora edulis) e avaliou seu potencial preventivo sobre a dentina erodida. A solução polifenólica experimental foi obtida a partir de sementes de maracujá através de secagem e prensagem a frio. Material e método Trinta amostras de dentina radicular cervical bovina foram adquiridas e randomizadas em três grupos (n=10): G1 – água destilada; G2 – solução experimental de antioxidantes fenólicos do maracujá; G3 – pasta de dente comercial anti-erosão. Ciclos erosivos e tratamento foram conduzidos ao longo de 3 dias. Posteriormente, microscopia confocal 3D sem contato foi empregada para medir a rugosidade volumétrica (Sa) e linear (Ra), bem como o desgaste erosivo. Um teste ANOVA/Tukey de uma via foi realizado (α=0,05). Resultado O grupo G2 apresentou valores de Ra e Sa mais baixos em comparação com os outros grupos e demonstrou o menor desgaste erosivo em µm em comparação com G1 e G3. Conclusão A solução experimental baseada em polifenóis antioxidantes da Passiflora edulis mostrou desempenho promissor sobre a dentina erodida nesta investigação. No entanto, mais pesquisas são necessárias para estabelecer sua eficácia e potencial uso no desenvolvimento de um novo produto. nãocariosa não cariosa vida entanto excessivas dentárias frio n=10 n10 n 10 (n=10) G destilada antierosão. antierosão anti erosão. erosão anti-erosão dias Posteriormente D (Sa Ra, , (Ra) ANOVATukey ANOVA Tukey α=0,05. α005 α α=0,05 . 0 05 (α=0,05) investigação produto n=1 n1 1 (n=10 (Ra α00 α=0,0 (α=0,05 n= (n=1 α0 α=0, (α=0,0 (n= α=0 (α=0, (n α= (α=0 (α= (α

Abstract Introduction The non-carious degradation of the dentin matrix is a natural process throughout life; however, when associated with the presence of excessive acidic substances, it leads to lesions in dental structures. Objective This study developed an experimental solution based on antioxidant polyphenols from the yellow passion fruit seed (Passiflora edulis) and assessed its preventive potential on eroded dentin. The experimental polyphenolic solution was obtained from passion fruit seeds through drying and cold pressing. Material and method Thirty samples of bovine cervical root dentin were acquired and randomized into three groups (n=10): G1 – distilled water; G2 – experimental solution of phenolic antioxidants from passion fruit; G3 – Commercial anti-erosion toothpaste. Erosive cycling and treatment were conducted over 3 days. Subsequently, non-contact 3D confocal microscopy was employed to measure volumetric (Sa) and linear (Ra) roughness, as well as erosive wear. A one-way ANOVA/Tukey test was performed (α=0.05). Result The G2 group had lower Ra and Sa values compared to the other groups and demonstrated the lowest erosive wear in µm compared to G1 and G3. Conclusion: The experimental solution based on antioxidant polyphenols from Passiflora edulis showed promising performance on eroded dentin in this investigation. Nevertheless, further research is required to establish its effectiveness and potential use in developing a new product. noncarious non carious life however substances structures pressing n=10 n10 n 10 (n=10) G water antierosion anti erosion toothpaste days Subsequently noncontact contact D (Sa (Ra roughness oneway one way ANOVATukey ANOVA Tukey α=0.05. α005 α α=0.05 . 0 05 (α=0.05) Conclusion investigation Nevertheless product n=1 n1 1 (n=10 α00 α=0.0 (α=0.05 n= (n=1 α0 α=0. (α=0.0 (n= α=0 (α=0. (n α= (α=0 (α= (α

Resumo: Este estudo teve como objetivo estimar a prevalência da COVID-19 e identificar fatores sociais e estratégias preventivas associadas a essa doença em pessoas em situação de vulnerabilidade socioeconômica (catadores de lixo, imigrantes/refugiados e moradores de rua) em Goiânia, Estado de Goiás, na região central do Brasil. Trata-se de um estudo transversal realizado de julho a outubro de 2020. Foram considerados positivos para a covid-19 indivíduos que apresentaram um teste de anticorpos anti-SARS-COV-2 e/ou teste de RNA para SARS-COV-2 com resultado positivo. Foram realizadas análises de regressão univariável e múltipla para identificar as variáveis associadas à COVID-19. Dos 594 participantes, 47,3% eram catadores de lixo reciclável, 29,6% eram imigrantes/refugiados e 23,1% eram moradores de rua. A positividade foi de 14,1% para o RNA do SARS-CoV-2, 30,8% para o total de anti-SARS-CoV-2 e 39,4% para pelo menos um marcador da COVID-19. Entre os 541 indivíduos, ser imigrante/refugiado, não usar máscara cirúrgica e ter três ou mais pessoas dormindo no mesmo quarto foram fatores associados à infecção por SARS-CoV-2, enquanto confiar em informações sobre a pandemia, principalmente aquelas transmitidas por noticiários de TV, foi um indicador de proteção contra a COVID-19. Este estudo revelou desigualdades étnicas e socioeconômicas na prevalência da COVID-19 entre pessoas de baixa renda no Brasil. Além disso, uma alta prevalência de COVID-19 foi detectada nos três grupos. O desenvolvimento de novas estratégias para combater e prevenir doenças transmissíveis que afetam essa população é essencial para mitigar pandemias futuras e atuais. Resumo COVID19 COVID 19 COVID-1 imigrantesrefugiados imigrantes refugiados rua Goiânia Goiás Brasil Tratase Trata se 2020 covid19 covid covid-1 antiSARSCOV2 antiSARSCOV anti SARS COV 2 anti-SARS-COV- eou SARSCOV2 SARSCOV SARS-COV- positivo COVID19. 19. 59 participantes 473 47 3 47,3 reciclável 296 29 6 29,6 231 23 1 23,1 141 14 14,1 SARSCoV2, SARSCoV2 SARSCoV CoV 2, SARS-CoV-2 308 30 8 30,8 antiSARSCoV2 antiSARSCoV anti-SARS-CoV- 394 39 4 39,4 54 imigranterefugiado imigrante refugiado imigrante/refugiado pandemia TV disso grupos atuais COVID1 COVID- 202 covid1 covid- anti-SARS-COV SARS-COV 5 47, 29, 23, 14, SARS-CoV- 30, anti-SARS-CoV 39, 20 SARS-CoV

Abstract: This study aimed to estimate the prevalence and identify social factors and preventive strategies associated with the coronavirus disease 2019 (COVID-19) in socio and economically vulnerable people (recyclable waste pikers, immigrants/refugees, and homeless people) in Goiânia, Goiás State, Central-Western Brazil. A cross-sectional study was conducted from July 2020 to October 2020. COVID-19 positivity was defined as a positive total anti-SARS-COV-2 antibody test and/or RNA test for SARS-COV-2. Univariable and multiple regression analyses were performed to identify the variables associated with COVID-19. Of the 594 participants, 47.3% were recyclable waste pickers, 29.6% were immigrants/refugees, and 23.1% were homeless people. The positivity for SARS-CoV-2 RNA was 14.1%, whereas for anti-SARS-CoV-2 a total of 30.8% were positive, and 39.4% were positive for at least one COVID-19 marker. Among the 541 individuals, being immigrants/refugees, not wearing a surgical mask, and having three or more people sleeping in the same room were associated with SARS-CoV-2 infection, while using TV news as the main source of information about the pandemic was a protective predictor of COVID-19. This study revealed ethnic and socioeconomic inequalities in the prevalence of COVID-19 among impoverished people in Brazil. Additionally, a high prevalence of COVID-19 was detected in all three groups. Developing new strategies to combat and prevent communicable diseases affecting this population is essential for mitigating future and ongoing pandemics. Abstract 201 COVID19 COVID 19 (COVID-19 pikers immigrantsrefugees immigrants refugees immigrants/refugees Goiânia State CentralWestern Central Western Brazil crosssectional cross sectional 202 COVID-1 antiSARSCOV2 antiSARSCOV anti SARS COV 2 anti-SARS-COV- andor SARSCOV2. SARSCOV2 SARSCOV 2. SARS-COV-2 COVID19. 19. 59 participants 473 47 3 47.3 pickers 296 29 6 29.6 231 23 1 23.1 SARSCoV2 SARSCoV CoV SARS-CoV- 141 14 14.1% antiSARSCoV2 antiSARSCoV anti-SARS-CoV- 308 30 8 30.8 394 39 4 39.4 marker 54 individuals mask infection Additionally groups pandemics 20 COVID1 (COVID-1 COVID- anti-SARS-COV SARS-COV- 5 47. 29. 23. SARS-CoV 14.1 anti-SARS-CoV 30. 39. (COVID- SARS-COV 14. (COVID

Resumen: Este estudio tuvo como objetivo estimar la prevalencia del COVID-19 e identificar los factores sociales y las estrategias preventivas asociadas a esta enfermedad en personas en situación de vulnerabilidad socioeconómica (recolectores de basura, inmigrantes/refugiados y personas sin hogar) en Goiânia, Estado de Goiás, en la región central de Brasil. Se trata de un estudio transversal realizado de julio a octubre de 2020. Las personas que tuvieron una prueba de anticuerpos anti-SARS-CoV-2 positiva y/o una prueba de ARN para SARS-CoV-2 se consideraron positivas para el COVID-19. Se realizaron análisis de regresión univariante y múltiple para identificar las variables asociadas al COVID-19. De los 594 participantes, el 47,3% eran recicladores; el 29,6% inmigrantes/refugiados; y el 23,1% eran personas sin hogar. La positividad fue del 14,1% para el ARN del SARS-CoV-2; del 30,8% para el total de anti-SARS-CoV-2; y del 39,4% para al menos un marcador del COVID-19. De los 541 participantes, ser inmigrante/refugiado, no usar mascarilla quirúrgica y tener tres o más personas que comparten la misma habitación fueron los factores asociados con la infección por SARS-CoV-2, mientras que confiar en la información sobre la pandemia, especialmente transmitidas por los noticiarios, fue un indicador de protección contra esta enfermedad. Este estudio reveló las desigualdades étnicas y socioeconómicas en la prevalencia del COVID-19 entre las personas de bajos ingresos en Brasil. Además, se detectó una alta prevalencia del COVID-19 en los tres grupos. El desarrollo de nuevas estrategias para combatir y prevenir las enfermedades transmisibles que afectan a esta población es fundamental para mitigar las pandemias futuras y actuales. Resumen COVID19 COVID 19 COVID-1 recolectores basura inmigrantesrefugiados inmigrantes refugiados hogar Goiânia Goiás Brasil 2020 antiSARSCoV2 antiSARSCoV anti SARS CoV 2 anti-SARS-CoV- yo SARSCoV2 SARSCoV SARS-CoV- COVID19. 19. 59 participantes 473 47 3 47,3 recicladores 296 29 6 29,6 231 23 1 23,1 141 14 14,1 308 30 8 30,8 394 39 4 39,4 54 inmigranterefugiado inmigrante refugiado inmigrante/refugiado SARSCoV2, 2, pandemia noticiarios Además grupos actuales COVID1 COVID- 202 anti-SARS-CoV SARS-CoV 5 47, 29, 23, 14, 30, 39, 20

ABSTRACT Objective To evaluate, characterize and search for trends in the underreporting of diabetes mellitus (DM) as the cause of death in Bauru, São Paulo, Brazil, over 40 years. Subjects and methods This was a documental study. Clinical and mortality data were collected from individuals known to have type 1 (DM1) and type 2 diabetes mellitus (DM2), residing in Bauru, State of São Paulo, followed at a local endocrinology clinic from 1982 to 2021, who deceased during this period. Results A significant underreporting of DM as the cause of death (64.41%) was found, mostly associated with male gender (OR = 1.59 [95% CI: 1.18; 2.15]; p < 0.01), DM2 (OR = 2.64 [95% CI: 1.32; 5.26]; p < 0.01), dying in the first decade of the study (OR = 4.07 [95% CI: 1.54; 10.71]; p < 0.001) and shorter DM duration (OR = 1.02 [95% CI: 1.01; 1.04]; p < 0.01). Age, type of treatment, body mass index, marital status and ethnicity, did not show a significant association with DM underreporting. There was a decreasing trend in DM1 underreporting (Decade Percentual Change = -7.10 [95% CI: -11.35; -3.40]), but a stationary trend for DM and DM2. The main primary cause of death was cardiovascular-related complications. Conclusion The underreporting of DM as the cause of death was very frequently found, and was associated with male gender, decade of death, shorter DM duration and DM2. If our data could be applied to the whole country, DM would possibly emerge as a more prominent cause of death in Brazil. Future studies in other cities and geographic regions are warranted to confirm our findings. evaluate (DM Bauru Paulo Brazil 4 years (DM1 DM2, , (DM2) 198 2021 period 64.41% 6441 64 41 (64.41% found OR 159 59 1.5 95% 95 [95 CI 1.18 118 18 2.15 215 15 2.15] 0.01, 001 0.01 0 01 0.01) 264 2.6 1.32 132 32 5.26 526 5 26 5.26] 407 07 4.0 1.54 154 54 10.71 1071 10 71 10.71] 0.001 0001 102 02 1.0 1.01 101 1.04 104 04 1.04] 0.01. . Age treatment index ethnicity Decade 7.10 710 7 -7.1 11.35 1135 11 35 -11.35 3.40, 340 3.40 3 -3.40]) cardiovascularrelated cardiovascular related complications country findings (DM2 19 202 64.41 644 6 (64.41 1. 9 [9 1.1 2.1 21 00 0.0 2. 1.3 13 5.2 52 4. 10.7 107 0.00 000 7.1 -7. 11.3 113 -11.3 34 3.4 -3.40] 20 64.4 (64.4 [ 0. 5. 10. 7. -7 11. -11. 3. -3.40 64. (64. - -11 -3.4 (64 -1 -3. (6 -3 (

RESUMO: O transtorno de acumulação de animais, principalmente de cães e gatos, é uma questão complexa que ocorre em quase todas as comunidades, sendo necessário o levantamento de dados que contribuam para a compreensão da leptospirose dentro da iniciativa Saúde Única. Com o objetivo de determinar a soroprevalência deLeptospirasp. em cães e gatos provenientes de acumuladores de animais foram utilizados 71 cães e 39 gatos de 33 acumuladores do município de Patos, estado da Paraíba, semiárido do Nordeste brasileiro, utilizando-se o teste de soroaglutinação microscópica (SAM) como teste de diagnóstico. Trinta e oito animais (34,5%; IC 95% = 25,7 - 43,4%) foram sororreativos, sendo 46,5% em cães e 12,8% em gatos (P < 0,01), com maior frequência do sorogrupo Icterohaemorrhagiae em ambas as espécies. A alta soroprevalência encontrada sugere a necessidade de políticas públicas para esta população vulnerável no sentido de prevenir a transmissão de zoonoses.

ABSTRACT: Animal hoarding disorder, especially dogs and cats, is a complex issue that occurs in almost all communities, and it is necessary to collect data that contribute to the understanding of leptospirosis within the One Health initiative. In order to determine the seroprevalence of Leptospirasp., 71 dogs and 39 cats from 33 people with animal hoarding profile in the municipality of Patos, state of Paraíba, semi-arid region of Northeastern Brazil were evaluated by using the microscopic serum agglutination test (MAT) as diagnostic test. Thirty-eight animals (34.5%; 95% CI = 25.7 - 43.4%) were seroreactive, with 46.5% in dogs and 12.8% in cats (P < 0.01), with the highest frequency of the Icterohaemorrhagiae serogroup in both species. The high seroprevalence reported suggests the need for public policies for this vulnerable population in order to prevent the transmission of zoonoses.

RESUMEN Objetivo: Evaluar el efecto de la infección por SARS-CoV-2 sobre las condiciones de salud y la capacidad funcional de personas mayores que viven en unidades de cuidados a largo plazo en la ciudad de Maceió – Alagoas. Métodos: Se realizó una cohorte prospectiva con ancianos institucionalizados de ambos sexos. Los ancianos fueron evaluados en cuanto a condiciones clínicas (diagnóstico de enfermedades crónicas y pruebas bioquímicas), capacidad funcional y estado nutricional. Todas las evaluaciones se repitieron en dos ocasiones, manteniendo un intervalo de 6 meses entre ellas. Resultados: La muestra estuvo compuesta por 289 personas mayores. Del total, 98 (33,9%) dieron positivo a la COVID-19 y ocho fallecieron (2,8%). Los hombres tenían más probabilidades de tener COVID-19 (OR = 3,50; p <0,01). Se observó que la enfermedad contribuyó al aumento de la frecuencia de ancianos dependientes después de seis meses (OR = 1,38; interacción p < 0,01). Se observó que la enfermedad contribuyó al aumento de la frecuencia de ancianos dependientes después de seis meses (OR = 1,38; interacción p < 0,01). También se observó que después de seis meses de diagnóstico positivo para COVID-19, hubo mayor pérdida de peso (p < 0,01), reducción del IMC (p < 0,01), aumento de la PAS media (p = 0,04) y la PAD (p = 0,03). Conclusión: Los efectos del COVID-19 en ancianos institucionalizados van más allá de las complicaciones agudas y comprometen el control de la presión arterial, la capacidad funcional, favoreciendo la pérdida de peso. Objetivo SARSCoV2 SARSCoV SARS CoV 2 SARS-CoV- Alagoas Métodos sexos bioquímicas, bioquímicas , bioquímicas) nutricional ocasiones ellas Resultados 28 total 9 33,9% 339 33 (33,9% COVID19 COVID 19 COVID-1 2,8%. 2,8% . 8 (2,8%) OR 3,50 350 3 50 <0,01. 001 <0,01 0 01 <0,01) 1,38 138 1 38 0,01. 0,01 0,01) COVID19, 19, 0,01, 0,04 004 04 0,03. 003 0,03 03 0,03) Conclusión arterial SARS-CoV 33,9 (33,9 COVID1 COVID- 2,8 (2,8% 3,5 35 5 00 <0,0 1,3 13 0,0 33, (33, 2, (2,8 3, <0, 1, 0, (33 (2, <0 (3 (2 (

ABSTRACT Objective: To assess the effect of SARS-CoV-2 infection on the health conditions and functional capacity of older adults living in long-term care units in Maceió City – Alagoas State. Methods: A prospective cohort was conducted with institutionalized older adults of both sexes. Older adults were assessed for clinical conditions (diagnosis of chronic diseases and biochemical tests), functional capacity, and nutritional status. All assessments were repeated on two occasions, maintaining a 6-month interval between them. Results: The sample was composed of 289 older adults. Of the total, 98 (33.9%) were positive for COVID-19 and eight died (2.8%). Men were more likely to have COVID-19 (OR = 3.50; p < 0.01). It was observed that the disease contributed to increasing the frequency of dependent older adults after six months (OR = 1.38; p-interaction < 0.01). It was also observed that after six months of positive diagnosis for COVID-19, there was greater weight loss (p < 0.01), reduced BMI (p < 0.01), increased mean SBP (p = 0.04), and DBP (p = 0.03). Conclusion: Effects of COVID-19 in institutionalized older adults go beyond acute complications and compromise blood pressure control, functional capacity, and favor weight loss. Objective SARSCoV2 SARSCoV SARS CoV 2 SARS-CoV- longterm long term State Methods sexes tests, tests , tests) status occasions 6month month 6 them Results 28 total 9 33.9% 339 33 (33.9% COVID19 COVID 19 COVID-1 2.8%. 2.8% . 8 (2.8%) OR 3.50 350 3 50 0.01. 001 0.01 0 01 0.01) 1.38 138 1 38 pinteraction interaction COVID19, 19, 0.01, 0.04, 004 0.04 04 0.04) 0.03. 003 0.03 03 0.03) Conclusion control SARS-CoV 33.9 (33.9 COVID1 COVID- 2.8 (2.8% 3.5 35 5 00 0.0 1.3 13 33. (33. 2. (2.8 3. 0. 1. (33 (2. (3 (2 (

RESUMO Objetivo: Avaliar o efeito da infecção pelo SARS-CoV-2 nas condições de saúde e capacidade funcional de idosos residentes em unidades de longa permanência na cidade de Maceió – Alagoas. Métodos: Foi realizada uma coorte prospectiva com idosos institucionalizados de ambos os sexos. Os idosos foram avaliados quanto às condições clínicas (diagnóstico de doenças crônicas e exames bioquímicos), capacidade funcional e estado nutricional. Todas as avaliações foram repetidas em duas ocasiões, mantendo um intervalo de 6 meses entre elas. Resultados: A amostra foi composta por 289 idosos. Do total, 98 (33,9%) deram positivo para COVID-19 e oito faleceram (2,8%). Os homens tiveram maior probabilidade de ter COVID-19 (OR = 3,50; p < 0,01). Observou-se que a doença contribuiu para o aumento da frequência de idosos dependentes após seis meses (OR = 1,38; interação de p <0,01). Observou-se também que após seis meses de diagnóstico positivo para COVID-19, houve maior perda de peso (p < 0,01), redução do IMC (p < 0,01), aumento da média da PAS (p = 0,04) e da PAD (p = 0,03). Conclusão: Os efeitos da COVID-19 em idosos institucionalizados vão além das complicações agudas e comprometem o controle da pressão arterial, a capacidade funcional, favorecendo a perda de peso. Objetivo SARSCoV2 SARSCoV SARS CoV 2 SARS-CoV- Alagoas Métodos sexos bioquímicos, bioquímicos , bioquímicos) nutricional ocasiões elas Resultados 28 total 9 33,9% 339 33 (33,9% COVID19 COVID 19 COVID-1 2,8%. 2,8% . 8 (2,8%) OR 3,50 350 3 50 0,01. 001 0,01 0 01 0,01) Observouse Observou se 1,38 138 1 38 <0,01. <0,01 <0,01) COVID19, 19, 0,01, 0,04 004 04 0,03. 003 0,03 03 0,03) Conclusão arterial SARS-CoV 33,9 (33,9 COVID1 COVID- 2,8 (2,8% 3,5 35 5 00 0,0 1,3 13 <0,0 33, (33, 2, (2,8 3, 0, 1, <0, (33 (2, <0 (3 (2 (

Resumo Introdução Os fisioterapeutas foram uma das categorias profissionais da linha de frente do combate à pandemia do coronavírus (COVID-19), todavia, pouco se sabe como esta pandemia afetou a saúde mental destes profissionais. Objetivo Analisar a relação entre a presença de transtorno mental comum (TMC) e elementos relacionados ao trabalho de fisioterapeutas que assistiram pacientes com COVID-19. Métodos Trata-se de um estudo transversal composto por fisioterapeutas de quatro estados do Brasil: Alagoas, Paraíba, Pernambuco e Rio Grande do Norte. As condições de trabalho avaliadas foram tipo de contrato, jornada semanal, tempo de trabalho, satisfação e aspectos psicossociais relacionados ao trabalho. A avaliação do TMC foi realizada por meio do Self-Reporting Questionnaire-20 (SRQ-20). Resultados Um total de 288 fisioterapeutas responderam ao formulário eletrônico, dos quais 76,2% tinham TMC. Medo de reinfecção (OR 2,75, IC 95%: 1,35-5,63) e infecção anterior por COVID-19 (OR: 2,03, IC 95%: 1,09-3,78) foram fatores relacionados a uma maior chance de risco de TMC. No entanto aqueles que relataram estarem satisfeitos com o trabalho (OR: 0,28, IC 95% 0,11-0,72) e os que tinham até 30 anos (OR: 0,46, IC 95% 0,24-0,88) tiveram menor chance de risco de TMC. Conclusão Os fisioterapeutas apresentaram maior prevalência de TMC. Medo de reinfecção e infecção prévia por COVID-19 foram considerados fatores de risco para TMC, enquanto a satisfação com o trabalho e ter mais que 30 anos foram fatores protetores ao TMC. COVID19, COVID19 COVID 19 , (COVID-19) todavia (TMC COVID19. 19. Tratase Trata Brasil Alagoas Paraíba Norte contrato semanal SelfReporting Self Reporting Questionnaire20 Questionnaire 20 Questionnaire-2 SRQ20. SRQ20 SRQ . (SRQ-20) 28 eletrônico 762 76 2 76,2 OR 275 75 2,75 95 1,355,63 135563 1,35 5,63 1 35 5 63 1,35-5,63 COVID-1 203 03 2,03 1,093,78 109378 1,09 3,78 09 3 78 1,09-3,78 028 0 0,28 0,110,72 011072 0,11 0,72 11 72 0,11-0,72 046 46 0,46 0,240,88 024088 0,24 0,88 24 88 0,24-0,88 COVID1 (COVID-19 Questionnaire2 Questionnaire- SRQ2 (SRQ-20 7 76, 27 2,7 9 355 1,355,6 13556 135 1,3 563 5,6 6 1,35-5,6 COVID- 2,0 093 1,093,7 10937 109 1,0 378 3,7 1,09-3,7 02 0,2 110 0,110,7 01107 011 0,1 072 0,7 0,11-0,7 04 4 0,4 240 0,240,8 02408 024 088 0,8 8 0,24-0,8 (COVID-1 (SRQ-2 2, 1,355, 1355 13 1, 56 5, 1,35-5, 1,093, 1093 10 37 3, 1,09-3, 0, 0,110, 0110 01 07 0,11-0, 0,240, 0240 08 0,24-0, (COVID- (SRQ- 1,355 1,35-5 1,093 1,09-3 0,110 0,11-0 0,240 0,24-0 (COVID (SRQ 1,35- 1,09- 0,11- 0,24-

Abstract Introduction The physiotherapists were one of the health professional categories on the Coronavirus (COVID-19) pandemic front line, however little is known how the pandemic has affected the mental health of these professionals. Objective To analyze the relation-ship between the presence of common mental disorder (CMD) and elements related to the work of physiotherapists who have been assisting patients with COVID-19. Methods: This is a cross-sectional survey study composed by physiotherapists from four states of Brazil: Alagoas, Paraíba, Pernambuco and Rio Grande do Norte. Working conditions evaluated were type of contract, weekly worked hours, time working, job satisfaction, and psychosocial aspects related to work. The CMD assessment was performed using the Self-Reporting Questionnaire-20 (SRQ-20). Results A total of 288 physiotherapists answered the electronic form, out of which 76.2% had CMD. Fear of reinfection (OR 2.75, 95%CI: 1.35-5.63) and previous infection by COVID-19 (OR: 2.03, 95%CI: 1.09-3.78) were factors related to a higher chance of risk of CMD. However, those who reported being satisfied with the work (OR: 0.28, 95%CI 0.11-0.72) and being more than 30 years old (OR: 0.46, 95%CI 0.24-0.88) had a lower chance of CMD risk. Conclusion Physiotherapists showed a higher prevalence of CMD. Fear of reinfection and previous infection by COVID-19 were risk factors to CMD, while satisfaction with the work and being more than 30 years old were protective factors to CMD. COVID19 COVID 19 (COVID-19 line professionals relationship relation ship (CMD COVID19. 19. Methods crosssectional cross sectional Brazil Alagoas Paraíba Norte contract hours working SelfReporting Self Reporting Questionnaire20 Questionnaire 20 Questionnaire-2 SRQ20. SRQ20 SRQ . (SRQ-20) 28 form 762 76 2 76.2 OR 275 75 2.75 95CI CI 95 1.355.63 135563 1.35 5.63 1 35 5 63 1.35-5.63 COVID-1 203 03 2.03 1.093.78 109378 1.09 3.78 09 3 78 1.09-3.78 However 028 0 0.28 0.110.72 011072 0.11 0.72 11 72 0.11-0.72 046 46 0.46 0.240.88 024088 0.24 0.88 24 88 0.24-0.88 COVID1 (COVID-1 Questionnaire2 Questionnaire- SRQ2 (SRQ-20 7 76. 27 2.7 9 355 1.355.6 13556 135 1.3 563 5.6 6 1.35-5.6 COVID- 2.0 093 1.093.7 10937 109 1.0 378 3.7 1.09-3.7 02 0.2 110 0.110.7 01107 011 0.1 072 0.7 0.11-0.7 04 4 0.4 240 0.240.8 02408 024 088 0.8 8 0.24-0.8 (COVID- (SRQ-2 2. 1.355. 1355 13 1. 56 5. 1.35-5. 1.093. 1093 10 37 3. 1.09-3. 0. 0.110. 0110 01 07 0.11-0. 0.240. 0240 08 0.24-0. (COVID (SRQ- 1.355 1.35-5 1.093 1.09-3 0.110 0.11-0 0.240 0.24-0 (SRQ 1.35- 1.09- 0.11- 0.24-

Abstract The TP53 3’UTR variant rs78378222 A>C has been detected in different tumor types as a somatic alteration that reduces p53 expression through modification of polyadenylation and miRNA regulation. Its prevalence is not yet known in all tumors. Herein, we examine tumor tissue prevalence of rs7837822 in Brazilian cohorts of patients from south and southeast regions diagnosed with lung adenocarcinoma (LUAD, n=586), sarcoma (SARC, n=188) and uterine leiomyoma (ULM, n=41). The minor allele (C) was identified in heterozygosity in 6/586 LUAD tumors (prevalence = 1.02 %) and none of the SARC and ULM samples. Additionally, next generation sequencing analysis revealed that all variant-positive tumors (n=4) with sample availability had additional pathogenic or likely pathogenic somatic variants in the TP53 coding regions. Among them, 3/4 (75 %) had the same pathogenic or likely pathogenic sequence variant (allele frequency <0.05 in tumor DNA) namely c.751A>C (p.Ile251Leu). Our results indicate a low somatic prevalence of rs78378222 in LUAD, ULM and SARC tumors from Brazilian patients, which suggests that no further analysis of this variant in the specific studied regions of Brazil is warranted. However, these findings should not exclude tumor molecular testing of this TP53 3’UTR functional variant for different populations. TP TP5 3UTR UTR 3 rs AC A C p p5 regulation Herein rs783782 (LUAD n=586, n586 n n=586 , 586 n=586) SARC, (SARC n=188 n188 188 ULM, (ULM n=41. n41 n=41 . 41 n=41) (C 6586 6 6/58 102 1 02 1.0 % samples Additionally variantpositive positive n=4 n4 4 (n=4 them 34 3/ 75 (7 005 0 05 <0.0 DNA c751AC cAC c 751A p.Ile251Leu. pIle251Leu pIleLeu p.Ile251Leu Ile251Leu Ile Leu (p.Ile251Leu) warranted However populations rs78378 n58 n=58 58 n=18 n18 18 658 6/5 10 1. n= (n= 7 ( 00 <0. pIle IleLeu (p.Ile251Leu rs7837 n5 n=5 5 n=1 n1 65 6/ (n <0 rs783 < rs78 rs7