Resumo O objetivo deste estudo foi avaliar o efeito da apicectomia realizada com broca ou inserto ultrassônico na adaptação do material obturador á dentina do canal radicular. Vinte incisivos inferiores humanos extraídos foram selecionados e preparados com o sistema ProDesign Logic até o diâmetro 40/.05. Os canais radiculares foram obturados com Bio-C Sealer (Angelus, PR, Brasil) por meio da técnica de cone único e foram armazenados em estufa a 37ºC e umidade relativa de 95% por 7 dias. Após esse período, foi realizada apicectomia dos 3 milímetros apicais utilizando broca Endo-Z (Maillefer - Dentsply) ou inserto ultrassônico BladeSonic (Helse Ultrasonic, Santa Rosa de Viterbo, Brasil) (n=10). Os espécimes foram escaneados por meio de microtomografia computadorizada (micro-CT; SkyScan 1176) com tamanho de voxel de 8,74 µm após obturação e após apicectomia. Please, correct this sentece to O percentual de falhas na interface entre o material obturador e a parede dentinaria foi avaliado nos 4 mm apicais da raiz apos apicectomia, alem do tempo de corte para ambos dispositivos. Os dados obtidos foram submetidos aos testes t pareado e não pareado (α = 0,05). Endo-Z e Bladesonic aumentaram o percentual de vazios entre o material obturador e a parede da dentina após apicectomia (p<0.05), sendo semelhantes entre si (p>0.05). Endo-Z demonstrou menor tempo para realizar apicectomia em comparação com Bladesonic (p<0.05). Apicectomias com Endo-Z ou Bladesonic podem prejudicar a interface material obturador/dentina, sendo a broca Endo-Z mais rápida que a ponta ultrassônica Bladesonic. radicular 4005 40 05 40/.05 BioC Bio C Angelus, Angelus (Angelus PR Brasil ºC 95 dias período EndoZ Endo Z Maillefer Dentsply Helse Ultrasonic Viterbo n=10. n10 n n=10 . 10 (n=10) microCT micro CT (micro-CT 1176 874 8 74 8,7 Please dispositivos α 0,05. 005 0,05 0 0,05) p<0.05, p005 p p<0.05 , (p<0.05) p>0.05. p>0.05 (p>0.05) p<0.05. obturadordentina obturador/dentina 400 40/.0 9 n1 n=1 1 (n=10 117 87 8, 00 0,0 p00 p<0.0 (p<0.05 p>0.0 (p>0.05 40/. n= (n=1 11 0, p0 p<0. (p<0.0 p>0. (p>0.0 40/ (n= p<0 (p<0. p>0 (p>0. (n p< (p<0 p> (p>0 (p< (p> (p

Abstract This study aimed to evaluate the effect of apicoectomy performed with a bur or an ultrasonic tip on the adaptation of the filling material to root canal dentin. Twenty human mandibular incisors were selected and prepared with a ProDesign Logic system up to diameter 40/.05. The root canals were filled with Bio-C Sealer (Angelus, PR, Brazil) using the single cone technique and were stored at 37ºC and 95% relative humidity for 7 days. After this period, the apicoectomy of the 3 millimeters of the root apex was performed using Endo-Z bur (Dentsply Sirona) or Bladesonic ultrasonic tip (Helse Ultrasonic, Santa Rosa de Viterbo, Brazil) (n=10). The specimens were scanned by micro-computed tomography (micro-CT; SkyScan 1176) with a voxel size of 8.74 µm after obturation and after apicoectomy. The percentage of voids at the interface between the filling material and the root canal wall was evaluated in the apical 4 mm of the root after apicoectomy, besides the time cutting to both devices. The data obtained were submitted to paired and unpaired t-tests (α = 0.05). Endo-Z and Bladesonic increased the percentage of voids between the filling material and the dentin after apicectomy (p<0.05), and they were similar (p>0.05). Endo-Z has a shorter time to perform apicoectomy compared to Bladesonic (p<0.05). Apicectomies with Endo-Z or Bladesonic could harm the material/dentin interface, while the Endo-Z bur was faster than the Bladesonic ultrasonic tip. 4005 40 05 40/.05 BioC Bio C Angelus, Angelus (Angelus PR Brazil ºC 95 days period EndoZ Endo Z Dentsply Sirona Helse Ultrasonic Viterbo n=10. n10 n n=10 . 10 (n=10) microcomputed micro computed microCT CT (micro-CT 1176 874 8 74 8.7 devices ttests t tests α 0.05. 005 0.05 0 0.05) p<0.05, p005 p p<0.05 , (p<0.05) p>0.05. p>0.05 (p>0.05) p<0.05. materialdentin 400 40/.0 9 n1 n=1 1 (n=10 117 87 8. 00 0.0 p00 p<0.0 (p<0.05 p>0.0 (p>0.05 40/. n= (n=1 11 0. p0 p<0. (p<0.0 p>0. (p>0.0 40/ (n= p<0 (p<0. p>0 (p>0. (n p< (p<0 p> (p>0 (p< (p> (p

Abstract In this study, we investigated the influence of the olfactive stimulus on visual attention. Two groups of 30 subjects participated in two experiments. Both experiments presented two arrays of fruits stimulus intercalated by an olfactive intervention. The stimulus was received in the form of images by the first group and in the form of words by the second group. An eye-tracking device monitored the timekeeping of visual attention dispensed in each stimulus. The results showed that olfactive priming influenced visual attention in both cases but with a greater degree in the images stimulus group. This study shows for the first time that image information is more susceptible to priming olfactive information than wording information. This effect may be associated with the formation of mental images in working memory, aroused by fragrances. 3 intervention eyetracking eye tracking memory fragrances

BACKGROUND:Herpes simplex virus (HSV) infection is an endemic disease and it is estimated that 6095% of the adult population are infected with symptoms that are usually self-limiting, though they can be serious, extensive and prolonged in immunocompromised individuals, highlighted by the emergence of drug-resistant strains. The study of the wild-type HSV strains based on the cytopathogenic features and its antiviral sensitivity are important in the establishment of an antivirogram for controlling the infection.OBJECTIVE:This study sought to isolate and examine the cytopathological characteristics of circulating strains of the Herpes simplex virus, from clinical specimens and their sensitivity to commercially available antiherpesvirus drugs, acyclovir, phosphonophormic acid and trifluridine.METHODS:Herpes simplex virus isolation, cytopathological features and antiviral sensitivity assays were performed in cell culture by tissue culture infectious dose or plaque forming unit assay.RESULTS:From twenty-two clinical specimens, we isolated and adapted nine strains. Overall, the cytopathic effect was detected 24 h post-infection (p.i.) and the presence of syncytia was remarkable 48 h p.i., observed after cell staining. Out of eight isolates, four developed plaques of varying sizes. All the isolates were sensitive to acyclovir, phosphonophormic and trifluridine, with the percentage of virus inhibition (%VI) ranging from 49.7-100%.CONCLUSIONS:The methodology for HSV isolation and characterization is a straightforward approach, but the drug sensitivity test, regarded as being of great practical importance, needs to be better understood.

Um estudo sorológico para Leptospira spp. foi realizado em caprinos leiteiros de 15 municípios do Rio Grande do Sul, Brasil. Foram examinados 354 soros frente a 12 sorovares de Leptospira pela técnica da aglutinação microscópica. No momento da coleta do sangue, todos os animais examinados possuíam aspecto saudável e não apresentavam sinais clínicos sugestivos de leptospirose. Dos animais amostrados, 3,4% apresentaram título 100 ou maior. Os sorovares icterohaemorragiae, pomona e hardjo foram encontrados nos animais soropositivos. Estes resultados confirmam a presença da infecção de caprinos por Leptospira no Rio Grande do Sul e indicam a existência de mais de um sorovar predominantemente nesta espécie na região.

A serological survey to detect antibodies against Leptospira spp. was conducted on dairy goats serum samples collected from 15 counties on the State of Rio Grande do Sul, Brazil. Serum samples collected from a total of 354 goats were tested against 12 serological Leptospira serovar, using the microscopic agglutination test. At the time of blood collection, all the animals were clinically examined and no clinical sign suggestive of Leptospirose was observed. The prevalence of positive serum, as determined at dilution of 1:100 or higher, was 3.4%. Agglutinins against three serovar (icterohaemorragiae, pomona and hardjo) were found in positive animals. These findings are suggestive of the presence of Leptospira infection on goats herds grazed on the State of Rio Grande do Sul and also does suggest the presence of more than one serovar on the herds studied.