Abstract Background: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the etiological agent of the disease coronavirus 2019 (COVID-19) in humans. SARS-CoV-2 has been identified in cats with or without clinical signs. Case presentation: We describe the pathological and molecular findings in a six-month-old asymptomatic cat with SARS-CoV-2 infection from Brazil, belonging to a human family with COVID-19 cases. The pool of nasopharynx and oropharynx swabs at day zero tested positive by RT-qPCR for SARS-CoV-2. No amplification resulted from molecular testing performed on days 7 and 14. The cat was hit by a car and died 43 days after the molecular diagnosis. Immunohistochemistry at post-mortem examination demonstrated nucleocapsid protein in samples from the lungs, kidneys, nasal conchae, trachea, intestine, brain and spleen. Conclusion: The present study has highlighted the possibility that viral antigens can be detected by immunohistochemistry in multiple organs six weeks after infection, although the same tissues tested negative by RT-PCR.

Abstract The aim of this study was to evaluate the blood flow of the uterine artery, fetal aorta and umbilical artery in the physiological pregnancy of sheep by means of pulsed Doppler throughout the gestational period. Thirty Santa Inês ewes weighing between 45.4±4.3 kg and aged 2 to 5 years were selected. The evaluations were carried out weekly from the 3rd to the 21st gestational week. Peak systolic velocity (PSV), end diastolic velocity (EDV) and resistance index (RI) were obtained. Analysis of variance was performed, and the minimum significant comparison of means was obtained by the BH test with adjusted P<0.05. The results were expressed as mean ± standard error. For the fetal aorta, there was an increase in the EDV values and a decrease in the PSV and RI throughout pregnancy. For the uterine artery, PSV and EDV did not present significant variation, whereas the RI showed a reduction in the last week. Increased EDV values were found for the umbilical artery throughout pregnancy. For the PSV there was no significant difference, as the RI was reduced at the end of pregnancy. The results obtained are expected to contribute to a more complete understanding of the hemodynamic changes resulting from pregnancy.

RESUMO Este estudo objetivou avaliar a mobilização energética em tambaqui submetido ao jejum, após a realimentação de um dia. 120 juvenis de tambaqui foram distribuídos em 12 caixas de polietileno de 310L. Foram avaliados três tratamentos: grupo controle (14 dias de alimentação); Jejum de 14 dias; e realimentado (13 dias em jejum e um dia de realimentação). Após o período experimental, os peixes foram anestesiados com eugenol para coleta de sangue e o soro e o plasma foram utilizados para dosagem de glicose, triglicerídeos, colesterol e proteína sérica. Posteriormente, os peixes foram eutanasiados para remoção de fígado e gordura mesentérica e foram usados na determinação de glicogênio e lipídio hepático e índice de gordura mesentérica. Os resultados foram submetidos à ANOVA e as médias comparadas pelo teste de Tukey quando observada significância estatística (P <0,05). Glicose, triglicérides e proteína sérica reduziram após o jejum, diferindo estatisticamente com o controle. Já a realimentação resultou na recuperação dos três indicadores sanguíneos. A análise no fígado mostra que o glicogênio foi consumido intensamente durante o jejum e recuperou parcialmente após a realimentação, quando comparados ao grupo controle. Os resultados obtidos neste estudo sugerem que o jejum de 14 dias não foi prejudicial aos peixes e os tambaqui são capazes de ajustar rapidamente seu metabolismo de acordo com seu estado nutricional.

ABSTRACT This study aimed evaluate energetic mobilization in tambaqui submitted to fasting, after a one-day refeeding. 120 tambaqui juveniles were distributed in 12 310L polyethylene boxes. Three treatments were evaluated: Control (14 days of feeding); Fasting for 14 days; and Refeeding (13 days fasting and one day of feedback). After the experimental period, the fish were anesthetized with eugenol for blood collection and serum and plasma were used to measure glucose, triglycerides, cholesterol and serum protein. Subsequently, fish were euthanized to remove liver and mesenteric fat and were used to determine hepatic glycogen and lipid and mesenteric fat index. The results were submitted to ANOVA and the means compared by Tukey test when statistical significance was observed (P <0.05). Glucose, triglycerides and serum protein decreased after fasting, differing statistically with the control. Refeeding resulted in the recovery of three blood indicators. Liver analysis shows glycogen was consumed intensely during fasting and partially recovered after refeeding, when compared to control group. The results obtained in this study suggest that the 14-day fast was not harmful to the fish and the tambaqui are able to quickly adjust their metabolism according to their nutritional status.

Abstract INTRODUCTION Blastocystis is an intestinal protozoan that may play a role in the pathogenicity of humans. This study aimed to (i) genetically characterize Blastocystis isolates obtained from human fecal samples and the water supply of the city of Uberaba, Minas Gerais, Brazil, and (ii) to verify the phylogenetic relationship between these isolates. METHODS Blastocystis species present in 26 fecal samples obtained from humans and animals from Uberaba were genetically characterized by polymerase chain reaction-restriction fragment length polymorphism and polymerase chain reaction-sequence-tagged sites. All amplicons were partially sequenced and/or defined according to the GenBank classification. RESULTS Polymerase chain reaction amplicons were generated from 21 human isolates and 18 water samples. The subtypes defined were ST1 (53.3%), ST3 (40.0%), and ST2 (6.7%) for human isolates; ST10 (100%) for bovine isolates; and ST5 (50.0%), ST1 (25%), and ST3 (25%) for pigs. Sequencing of polymerase chain reaction products showed a 98%-99% identity for the Blastocystis sequences deposited in GenBank, except for sequences from water samples that showed the identity of algae sequences. Phylogenetic analysis of Blastocystis sequences showed two distinct groups, one of which was principally formed by ST1, ST5, and ST10, and the other by isolates characterized as ST3 and ST7. Both clades showed human and animal sequences, reinforcing the notion that Blastocystis subtypes are not host-specific. CONCLUSIONS The data showed that Blastocystis subtypes circulating in Uberaba are ST1-ST3, ST5, and ST10, present in both humans and animals, demonstrating that the Blastocystis subtypes are not host-specific; that is, zoonotic transmission is possible.

Abstract Aim: The present study aimed to perform a literature review on the use of wearable inertial sensors for gait analysis of children in clinical practice. Methods: Searches were performed in the MEDLINE, EMBASE, Cochrane Library, and PEDro databases for studies involving children or adolescents submitted to gait analysis with the use of wearable inertial sensors. No restrictions were imposed regarding the date of publication or language. Results: Three hundred twenty articles were retrieved, 14 of which met the eligibility criteria and were selected for the present systematic review. Two independent reviewers assessed the risk of bias and study quality using the ROBINS-I and AXIS scale. The studies included in the present review reported multiple outcomes of kinematic gait assessments calculated from the signals provided by the wearable sensors, performed in a hospital setting, outpatient clinic, and a familiar environment, with several types of pediatric conditions. Conclusion: The findings suggest that wearable sensors are effective for the evaluation of quantitative gait variables in children with different pediatric conditions, enabling an objective analysis that should prove useful in the processes of clinical diagnosis and rehabilitation. However, given the relatively small number of studies published on this topic, it is difficult to make strong recommendations regarding the most appropriate equipment, sensor placement, and outcomes for assessing gait in children.

Abstract INTRODUCTION: Currently, there are few studies regarding Blastocystis epidemiology. This study aimed to evaluate the occurrence of Blastocystis in animals in Uberaba, Brazil. METHODS: Fecal samples were examined by parasitological methods and screened for Blastocystis by polymerase chain reaction. RESULTS: Blastocystis spp. were observed in pigs, sheep, cattle, and dogs. Blastocystis polymerase chain reaction was positive in 14/22 samples positive by parasitological methods. CONCLUSIONS: The occurrence of Blastocystis in animals is high, with a predominance of subtype 1 in the region. This is the first study conducted in Brazil showing the genetic profile of Blastocystis isolated from animals.

RESUMO Uma das formas de melhoria da qualidade e da produção do tomateiro é a adoção de técnicas adequadas de manejo da cultura, destacando-se o uso da enxertia e o método de condução das plantas. Contudo, no Brasil, há falta de informações que estimulem essas práticas. Por isso, este trabalho teve por objetivo avaliar o desempenho de porta-enxertos para tomateiro, com vigoroso sistema radicular, conduzido com quatro hastes, em fibra da casca de coco, em ambiente protegido. Adotou-se o espaçamento de 2,0 m entrelinhas e 0,40 m entre plantas. Para isso, foi utilizado o delineamento em blocos casualizados, com quatro tratamentos e seis repetições, sendo, os tratamentos, dois porta-enxertos (‘Maxifort’ e ‘Multifort’), autoenxertia (‘Alambra’ em ‘Alambra’), e o pé franco (‘Alambra’). Cada parcela foi constituída de 20 plantas, sendo avaliadas as 14 plantas centrais. Não houve efeito significativo no uso dos porta-enxertos sobre as características de produção e de qualidade dos frutos. Na avaliação do estado nutricional, houve diferenças para os teores de P, Mg e Ca em plantas enxertadas. Portanto, os porta-enxertos ‘Maxifort’ e ‘Multifort’, cultivados nas condições em que o experimento foi realizado, não proporcionaram aumento da produção e da qualidade dos frutos.

ABSTRACT One way of improving the quality and production of tomato is the adoption of appropriate techniques for crop management, especially the use of grafting and conduction method of the plants. However, in Brazil, there is a lack of information to encourage such practices. Therefore, this study aimed to evaluate the performance of rootstocks for tomato plants, with vigorous root system, conducted with four stems, in coconut fiber, under protected cultivation. The adopted spacing was 2.0 m between rows and 0.40 m between plants. For this, we used a randomized block design with four treatments and six replications. The treatments consisted of two rootstocks (“Maxifort” and “Multifort”), self-grafted (“Alambra” in “Alambra”), and ungrafted (“Alambra”). Each plot consisted of 20 plants, out of which the 14 central plants were evaluated. There was no significant effect on yield and fruit quality. In the assessment of nutritional status, there were no differences in the levels of P, Mg, and Ca in grafted plants. Therefore, the rootstocks Maxifort and Multifort, grown under conditions in which the experiment was conducted, did not increase the production and quality of fruits.

RESUMO Visando avaliar a compatibilidade de tomateiros enxertados em diferentes portaenxertos, sob ambiente protegido, foi determinada a atividade das enzimas antioxidantes, peroxidases (POXs), polifenoloxidase (PPO) e superóxido dismutase (SOD). A primeira etapa experimental consistiu na avaliação, aos seis e onze dias após a enxertia, das mudas enxertadas e não enxertadas com três combinações de enxertia (Maxifort, Multifort e autoenxertia), em três regiões de coleta no caule: a) 2 cm acima da região da enxertia; b) na região de enxertia e c) 2 cm abaixo da região da enxertia, além do tratamento adicional, constituído de mudas não enxertadas de 'Alambra' (pé franco). O delineamento experimental foi inteiramente casualizado, em esquema fatorial 3x3+1, com cinco repetições. Na segunda etapa experimental houve o transplantio das mudas para a casa de vegetação, com as combinações de enxertia 'Maxifort', 'Multifort', autoenxertia e pé franco, considerando nas parcelas as combinações da enxertia e nas subparcelas os quatro períodos de coleta 30, 60, 90 e 120 dias após o transplantio. O delineamento experimental foi em blocos ao acaso, em esquema de parcela subdividida, sendo cada parcela experimental constituída de seis plantas, com seis repetições. Os resultados da primeira etapa mostram que houve incremento na atividade das POXs e PPO durante o pegamento das enxertias com os portaenxertos 'Maxifort', 'Multifort' e também na autoenxertia. Na segunda etapa, observou-se maior incremento na atividade de SOD e POXs para o portaenxerto 'Multifort' aos 120 dias após o transplantio. As plantas enxertadas e o pé franco não apresentaram indícios de incompatibilidade, sendo o aumento da atividade enzimática atribuído ao processo de cicatrização e lignificação das mudas enxertadas, tendo mantido esse comportamento ao longo do ciclo de crescimento e desenvolvimento das plantas de tomateiro. A produção por planta não sofreu influência da enxertia.

ABSTRACT To evaluate the compatibility of tomato plants grafted onto different rootstocks under greenhouse conditions, the activity of antioxidant enzymes, peroxidases (POXs), polyphenol oxidase (PPO) and superoxide dismutase (SOD) were determined. The first experimental step was to assess, at six and eleven days after grafting, the grafted seedlings and non-grafted ones with three combinations of grafting (Maxifort, Multifort and selfgraft) in three collecting regions in the stem: a) 2 cm above the grafting region; b) on the grafting region c) 2 cm below the grafting region, in addition to further treatment, consisting of ungrafted seedlings of 'Alambra'. The experimental design was completely randomized in a factorial 3x3+1, with five replications. The second experimental step was transplanting the seedlings to the greenhouse, with the combinations of grafting 'Maxifort', 'Multifort', selfgraft and ungrafted, considering the plots combinations of grafting and the subplots the four collection periods 30 60, 90 and 120 days after transplanting. The experimental design was a randomized block in a split plot scheme, each experimental plot consisted of six plants, with six replications. The results of the first stage showed an increase in the activity of POXs and PPO during grafting fixation with rootstocks 'Maxifort', 'Multifort' and also in the selfgraft. In the second stage, there was a higher increase in SOD and POXs activity for the rootstock 'Multifort' at 120 days after transplanting. The grafted and ungrafted plants showed no signs of incompatibility, and the increased enzyme activity attributed to the healing process and lignification of grafted seedlings, having kept this behavior over the cycle of growth and development of tomato plants.

OBJETIVO: descrever o perfil dos agravos relacionados ao trabalho notificados no estado do Rio Grande do Norte, Brasil, de 2007 a 2009. MÉTODOS: estudo descritivo, com dados do Sistema de Informação de Agravos de Notificação do Centro de Referência em Saúde do Trabalhador. RESULTADOS: dos 167 municípios, 40,1% tiveram notificações de doenças e agravos, entre acidentes biológicos (53,7%), acidentes graves (38,4%), distúrbios oesteomusculares (3,1%), intoxicações exógenas (2,6%), transtornos mentais (1,5%), pneumoconioses (0,5%) e dermatoses (0,2%); entre acidentados, predominaram trabalhadores do sexo masculino (53,7%), de 25 a 44 anos de idade (62,1%), escolaridade até o Ensino Médio (25,9%), com emprego registrado (23,9%); incapacidades temporárias corresponderam a 32,0% dos registros. CONCLUSÃO: evidenciou-se o perfil e o impacto das doenças e agravos ocupacionais no estado e a importância de dados consistentes e oportunos para seu monitoramento sistemático e o planejamento das ações em Saúde do Trabalhador.

OBJECTIVE: to describe the epidemiological profile of notified work-related health problems in Rio Grande do Norte State, Brazil, 2007-2009. METHODS: this was a descriptive study using Occupational Health Reference Center data kept on the Notifiable Diseases Information System. RESULTS: 40.1% of the 167 municipalities notified occupational diseases, as follows: biological accidents (53.7%), serious accidents (38.4%), Musculoskeletal Disorders (3.1%), exogenous poisoning (2.6%), mental disorders (1.5%), pneumoconiosis (0.5%) and dermatoses (0.2%). The most affected were male workers (53.7%), people aged 25-44 (62.1%), those with middle school education (25.9%) and those having formal employment (23.9%). 32.0% of cases reported temporary incapacity. CONCLUSION: the profile and impact of occupational injuries and diseases in the State were revealed, as was the importance of consistent and timely data for their systematic monitoring and planning of Occupational Health actions.

OBJETIVO: describir el perfil de los agravamientos relacionados al trabajo notificados en el estado de Rio Grande do Norte, Brasil, de 2007 a 2009. MÉTODOS: estudio descriptivo, con datos del Sistema de Información de Agravamientos de Notificación del Centro de Referencia en la Salud del Trabajador. RESULTADOS: de los 167 municipios, 40,1% tuvieron notificación de enfermedades y agravamientos, entre accidentes biológicos (53,7%), accidentes graves (38,4%), molestias osteomusculares (3,1%), intoxicaciones exógenas (2,6%), trastornos mentales (1,5%), neumoconiosis (0,5%) y dermatosis (0,2%); entre accidentados, predominaron trabajadores del sexo masculino (53,7%), de 25 a 44 años de edad (62,1%), escolaridad hasta la Enseñanza Secundaria (25,9%), con empleo registrado (23,9%); incapacidades temporales correspondieron a 32,0% de los registros. CONCLUSIÓN: se evidenció el perfil y el impacto de las enfermedades y agravamientos ocupacionales en el estado y la importancia de datos consistentes y oportunos para su monitoreo sistemático y para la planificación de las acciones en la Salud del Trabajador.

OBJETIVO:descrever o perfil dos agravos relacionados ao trabalho notificados no estado do Rio Grande do Norte, Brasil, de 2007 a 2009.MÉTODOS:estudo descritivo, com dados do Sistema de Informação de Agravos de Notificação do Centro de Referência em Saúde do Trabalhador.RESULTADOS:dos 167 municípios, 40,1% tiveram notificações de doenças e agravos, entre acidentes biológicos (53,7%), acidentes graves (38,4%), distúrbios oesteomusculares (3,1%), intoxicações exógenas (2,6%), transtornos mentais (1,5%), pneumoconioses (0,5%) e dermatoses (0,2%); entre acidentados, predominaram trabalhadores do sexo masculino (53,7%), de 25 a 44 anos de idade (62,1%), escolaridade até o Ensino Médio (25,9%), com emprego registrado (23,9%); incapacidades temporárias corresponderam a 32,0% dos registros.CONCLUSÃO:evidenciou-se o perfil e o impacto das doenças e agravos ocupacionais no estado e a importância de dados consistentes e oportunos para seu monitoramento sistemático e o planejamento das ações em Saúde do Trabalhador.

OBJECTIVE:to describe the epidemiological profile of notified work-related health problems in Rio Grande do Norte State, Brazil, 2007-2009.METHODS:this was a descriptive study using Occupational Health Reference Center data kept on the Notifiable Diseases Information System.RESULTS:40.1% of the 167 municipalities notified occupational diseases, as follows: biological accidents (53.7%), serious accidents (38.4%), Musculoskeletal Disorders (3.1%), exogenous poisoning (2.6%), mental disorders (1.5%), pneumoconiosis (0.5%) and dermatoses (0.2%). The most affected were male workers (53.7%), people aged 25-44 (62.1%), those with middle school education (25.9%) and those having formal employment (23.9%). 32.0% of cases reported temporary incapacity.CONCLUSION:the profile and impact of occupational injuries and diseases in the State were revealed, as was the importance of consistent and timely data for their systematic monitoring and planning of Occupational Health actions.

OBJETIVO:describir el perfil de los agravamientos relacionados al trabajo notificados en el estado de Rio Grande do Norte, Brasil, de 2007 a 2009.MÉTODOS:estudio descriptivo, con datos del Sistema de Información de Agravamientos de Notificación del Centro de Referencia en la Salud del Trabajador.RESULTADOS:de los 167 municipios, 40,1% tuvieron notificación de enfermedades y agravamientos, entre accidentes biológicos (53,7%), accidentes graves (38,4%), molestias osteomusculares (3,1%), intoxicaciones exógenas (2,6%), trastornos mentales (1,5%), neumoconiosis (0,5%) y dermatosis (0,2%); entre accidentados, predominaron trabajadores del sexo masculino (53,7%), de 25 a 44 años de edad (62,1%), escolaridad hasta la Enseñanza Secundaria (25,9%), con empleo registrado (23,9%); incapacidades temporales correspondieron a 32,0% de los registros.CONCLUSIÓN:se evidenció el perfil y el impacto de las enfermedades y agravamientos ocupacionales en el estado y la importancia de datos consistentes y oportunos para su monitoreo sistemático y para la planificación de las acciones en la Salud del Trabajador.

O mogno (Swietenia macrophylla King), pelo elevado valor comercial da sua madeira, é uma das espécies mais exploradas na Amazônia, sendo ameaçada de extinção por não haver renovação dos estoques através de reflorestamento com a espécie. O objetivo deste trabalho foi avaliar o efeito da adubação fosfatada sobre o desenvolvimento de mudas de mogno. O estudo foi conduzidos em casa-de-vegetação e o solo utilizado para compor o substrato foi Latossolo Amarelo de textura muito argilosa. Os tratamentos constituiram-se de doses crescentes de fósforo de 0, 25, 30, 75, 100, 150 e 200 kg.ha-1 de P. O delineamento experimental foi o de blocos casualizados com 4 repetições, num total de 28 parcelas. Cada parcela foi formada por 2 mudas, cultivadas em sacos com capacidade de 4 dm³. Verificaram-se respostas positivas às doses de fósforo para todas as características de crescimento, bem como na absorção da maioria dos macronutrientes. Para as características de crescimento a dose de 200 kg.ha-1 de fósforo, foi a que proporcionou máximo crescimento para as mudas de mogno, no intervalo de 90 dias.

ABSTRACT Due to the high value of mohogany woods (Swietenia macrophylla King), this specie is one of the most explored in the Amazon region. That explains the reason why mahogany is almost extinct.The present work evaluates the effect of phosphated fertilization on the mahogany seedlings under the increased doses of phosphorus. The experiment happened in a greenhouse and the soil used to compose the substratum was a yellow oxisoil with a very argillaceous texture. The treatments were made by doses of P: 0, 25, 30, 75, 100, 150 and 200 kg.ha-1 of P. The experimental design consisted of four randomized blocks, for a total of 28 parcels. Each parcel was formed by 2 seedlings, cultived in bags with 4 dm³ of soil. It was noticed positive answers by seedlings cultivated species for all the biometric characters analyzed and, in the same way, for the contents of almost the analyzed nutrients. For the growth characteristics, 200 kg.ha-1 was the dose that provided the high growth for mohogany, on the interval of 90 days.

O plantio de espécies florestais nativas é uma atividade que além de repor os recursos florestais também pode atenuar os impactos ambientais decorrentes do extrativismo. Entretanto, seu sucesso depende, entre outros fatores, do conhecimento a cerca das necessidades nutricionais da espécie a ser utilizada. Com o objetivo de obter informações das necessidades de mudas de mogno (Swietenia Macrophilla King) por micronutrientes, foi realizado um experimento em casa de vegetação. Foi utilizado como substrato um Latossolo Amarelo de baixa fertilidade, coletado da camada de 20-40 cm de profundidade, localizado no setor Sul do Campus da Universidade Federal do Amazonas (UFAM). Foram testados sete tratamentos e quatro repetições dispostos em delineamento experimental de blocos ao acaso, sob a técnica do elemento faltante. Os tratamentos foram: Completo (Calagem + N, P, K, Ca, Mg, S, B, Cu, Fe, Mn, Zn e Mo), Testemunha (Calagem + N, P, K, Ca, Mg, S e Mo) e a omissão de um micronutriente catiônico por vez (-B, -Cu, -Fe, -Mn e - Zn). Após quatro meses foram avaliadas as seguintes características: altura da parte aérea, diâmetro do colo, produção de matéria seca da parte aérea e das raízes e conteúdo de nutrientes na matéria seca da parte aérea. Os resultados obtidos nesta pesquisa permitiram concluir que as mudas de mogno tiveram seu crescimento comprometido pela baixa disponibilidade de cobre no substrato, sendo necessária sua aplicação para que as plantas tenham um desenvolvimento normal, compatível com o crescimento da espécie, quando as condições de substrato são adequadas.

The planting of native forest species is an activity which in addition to replacing forest resources can also lessen the environmental impacts resulting from their extraction. However, its success depends, among other things, on the knowledge regarding the nutritional needs of the species to be utilized. An experiment was carried out in a greenhouse aiming to acquire information on the mahogany (Swietenia Macrophilla) seedling micronutrient requirements. A low fertility Yellow Latosol, collected from a 20-40 cm deeplayer, located at the University Federal of Amazonas (UFAM) Campus southern sector, was used as substract. We tested seven treatments an four replicated arranged in a random block experimental design, under the lacking element technique. Treatments were as follows (Liming + N, P, K, Ca, Mg, S, B, Cu, Fe, Mn, Zn and Mo), and the omission of a catiônico micronutrient at a time (-B, -Cu, - Fe, -Mn and - Zn). The following features were assessed after four months: aerial part height, diameter, and aerial part and root dry matter yield. The resulted obtained in this research allowed to conclude that the mahogany seedlings had their growth compromised by the low availability of cooper in the substract, thus its application is necessary so that the plants would a normal growing, compatible with the their usual growth, when the substract conditions were appriate.