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Resumo O gênero Tetranematichthys possui apenas três espécies, e nenhuma delas tinha sido submetida a análises citogenéticas. Assim, este estudo traz pela primeira vez a análise de Tetranematichthyswallacei, coletado no Igarapé Apaú, bacia do rio Guamá, cidade de Castanhal, estado do Pará, Brasil. O número diploide de 52 cromossomos (32m+18sm+2st, NF = 104) foi encontrado em ambos os sexos, com heterocromatina predominantemente terminal e algumas intersticiais. A sequência telomérica foi observada exclusivamente em regiões terminais. Os sítios de 18S rDNA foram encontrados no par 17sm de todos os exemplares e em apenas um dos homólogos do par 7 em três exemplares. Os sítios de 5S rDNA foram encontrados nos pares 8m e 10m. Tetranematichthyswallacei possui algumas características que dignas de quanto à sua posição filogenética atual, incluindo uma provável redução no número diploide. Além disso, T.wallacei compartilha com Tympanopleuraatronasus o 18S rDNA alocado no braço longo de um grande cromossomo sm (primeiro par), mas não compartilha com Ageneiosus o primeiro par m grande com evidências de fusão, como observado em Ageneiosus inermis. Os dados cromossômicos gerados para T. wallacei, juntamente com os dados dos outros dois táxons de Ageneiosini estudados anteriormente, reforçam propostas de estudos baseados em morfologia que sugerem que a tribo representa o clado mais diferenciado dentro da família. espécies citogenéticas Assim Apaú Guamá Castanhal Pará Brasil 5 32m+18sm+2st, 32m18sm2st msmst 32m 18sm 2st st (32m+18sm+2st 104 sexos intersticiais terminais S 10m atual disso Twallacei T wallacei par, , par) fusão inermis anteriormente família 32m+18sm+2st 10 1

Abstract The genus Tetranematichthys has only three species, and none of them have undergone cytogenetic analyses. Therefore, this study brings for the first time the analysis of Tetranematichthys wallacei, collected from the Igarapé Apaú, Guamá River basin, municipality of Castanhal, Pará State, Brazil. The diploid number found was 52 chromosomes (32m+18sm+2st, NF = 104), in both sexes, with predominantly terminal and some interstitial heterochromatin. Telomeric sequences were observed exclusively in terminal regions. The 18S rDNA sites were found on pair 17sm of all specimens and in only one of the homologous of pair 7 in three specimens. The 5S rDNA sites were found in pairs 8m and 10m. Tetranematichthys wallacei exhibits characteristics worthy of attention regarding its current phylogenetic position, including a probable diploid number reduction. Additionally, it shares with Tympanopleura atronasus the 18S rDNA allocated in the long arm of a large sm chromosome (first pair) but does not share with Ageneiosus the large first m pair with evidence of fusion, as observed in Ageneiosus inermis. The chromosomal data generated for T. wallacei, along with the data from the other two previously studied Ageneiosini taxa, reinforces proposals from morphology-based studies suggesting that the tribe represents the most distinct clade within the family. species analyses Therefore Apaú basin Castanhal State Brazil 5 32m+18sm+2st, 32m18sm2st msmst 32m 18sm 2st st (32m+18sm+2st 104, 104 , 104) sexes heterochromatin regions S 10m position reduction Additionally fusion inermis T taxa morphologybased morphology based family 32m+18sm+2st 10 1

https://doi.org/10.1590/1982-0224-2024-0015

Efficiency of mixed and rigid occlusal stabilization splints: Randomized clinical trial splints
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OPPITZ, Layza Rossatto

; ARANTES, Ana Carolina Mastriani

; GARANHANI, Roberto Ramos

; COSTA, Carlos Alberto

; ARAUJO, Cristiano Miranda de

; TANAKA, Orlando Motohiro

; ANDREIS, Patricia Kern di Scala

; SCHAPPO, Claudia

; IGNÁCIO, Sérgio Aparecido

; JOHANN, Aline Cristina Batista Rodrigues

; RACHED, Rodrigo Nunes

; CAMARGO, Elisa Souza

Brazilian Oral Research

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2024, Volume 38 elocation e017

Abstract: En | Text: En | PDF: En

Abstract Occlusal stabilization splints are the most common treatment for controlling the deleterious effects of sleep bruxism. This study aimed to evaluate the effectiveness of a low-cost, mixed occlusal splint (MOS) compared to that of a rigid splint. A randomized clinical trial was performed on 43 adults of both sexes with possible sleep bruxism and satisfactory dental conditions. They were divided into rigid occlusal splint (ROS) (n = 23) and MOS (n = 20) groups. Masticatory muscle and temporomandibular joint (TMJ) pain intensity (visual analog scale), quality of life (WHOQOL-BREF), indentations in the oral mucosa, anxiety, and depression (HADS), number of days of splint use, and splint wear were evaluated. All variables were evaluated at baseline (T0), 6 months (T6), and 12 months (T12) after splint installation (T0), and splint wear was evaluated at T6 and T12. Student’s t-test, Mann–Whitney U test, non-parametric Friedman’s analysis of variance for paired samples and pairwise multiple comparisons, Pearson’s chi-square test, two-proportion z-test, non-parametric McNemar’s and Cochran’s Q, and Wilcoxon tests were used (p < 0.05). In both groups, there was a decrease in TMJ pain and pain intensity over time and improvements in the quality of life scores. At T6, there was a higher rate of splint wear in the MOS group than in the ROS group (p = 0.023). The MOS showed a higher rate of wear than the rigid splint but had similar results for the other variables. Therefore, the use of a mixed splint appears to be effective in controlling the signs and symptoms of sleep bruxism. lowcost, lowcost low cost, cost low-cost (MOS 4 conditions (ROS n 23 20 groups (TMJ visual scale, scale , scale) WHOQOLBREF, WHOQOLBREF WHOQOL BREF (WHOQOL-BREF) mucosa anxiety HADS, HADS (HADS) T0, T0 T (T0) (T6) 1 T12 (T12 Students Student s ttest, ttest t test t-test MannWhitney Mann Whitney nonparametric non parametric Friedmans Friedman comparisons Pearsons Pearson chisquare chi square twoproportion two proportion ztest, ztest z z-test McNemars McNemar Cochrans Cochran Q p 0.05. 005 0.05 . 0 05 0.05) scores 0.023. 0023 0.023 023 0.023) Therefore 2 (WHOQOL-BREF (HADS (T0 (T6 T1 (T1 00 0.0 002 0.02 02 (T 0.

https://doi.org/10.1590/1807-3107bor-2024.vol38.0017

Discrimination of cryptic species: Tabanus triangulum and Tabanus occidentalis (Diptera: Tabanidae) differ in size and shape species Diptera (Diptera Tabanidae
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Rodrigues, Gratchela Dutra

; Centeno Filho, Boaventura Lobo

; Morales, Diuliani Fonseca

; Dimer, Rafaela de Freitas Rodrigues Mengue

; Cavalheiro, Caroline da Silva

; Krolow, Tiago Kütter

; Moura, Mauricio Osvaldo

; Krüger, Rodrigo Ferreira

Revista Brasileira de Parasitologia Veterinária

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2024, Volume 33 N. 2 elocation e020123

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Resumo Fêmeas de mutucas (Diptera, Tabanidae) são hematófagas e podem ser vetores de patógenos que afetam animais de criação. Complexos de espécies crípticas são comuns em Tabanidae, como exemplificado por algumas espécies de Tabanus, incluindo Tabanus triangulum e Tabanus occidentalis, ambas prevalentes na região Sul do Brasil. Neste estudo foi utilizada morfometria geométrica para avaliar a venação das asas na identificação de espécies. Foi demonstrada que essa ferramenta diferencia, efetivamente, T. triangulum de T. occidentalis na Planície Costeira do Rio Grande do Sul, situada no bioma Pampa. Os resultados indicam que T. triangulum e T. occidentalis ocupam regiões distintas do espaço morfológico, permitindo sua identificação precisa por meio da morfometria geométrica, a qual é rápida, acessível e fácil de implementar. Diptera, Diptera (Diptera Tabanidae criação Brasil diferencia efetivamente T Pampa morfológico rápida implementar

Abstract Horse fly females (Diptera, Tabanidae) are hematophagous and can vector pathogens that affect livestock. Complexes of cryptic species are common in Tabanidae, as exemplified by some species of Tabanus, including Tabanus triangulum and Tabanus occidentalis, both prevalent in the Southern region of Brazil. In this study, geometric morphometrics were employed to ascertain the wing venation in species identification. It was demonstrated that this tool effectively differentiates T. triangulum from T. occidentalis in the coastal plain of Rio Grande do Sul state, situated within the Pampa biome. The results indicate that T. triangulum and T. occidentalis occupy distinct regions of the morphological space, allowing their precise identification through geometric morphometrics, which is fast, affordable, and easy to implement. Diptera, Diptera (Diptera Tabanidae livestock Brazil study T state biome space fast affordable implement

https://doi.org/10.1590/s1984-29612024028

Endoscopic endonasal approach for acromegaly: surgical outcomes using 2018 consensus criteria for remission acromegaly 201 20 2
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Cavalcante, Rodrigo Alves de Carvalho

; Vieira Netto, Luiz Alves

; Peres, Luís Felipe Araújo

; Zaccariotti, Alice Jardim

; Alencar, Helioenai de Sousa

; Jatene, Estela Muszkat

; Camargo, Leandro Azevedo

; Rodrigues, Monike Lourenço Dias

Archives of Endocrinology and Metabolism

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2023, Volume 67 N. 6 elocation e000650

Abstract: En | Text: En | PDF: En

ABSTRACT Objective: The primary aim is to analyze the endoscopic endonasal surgical results in short-term and two-year follow-ups according to the 11th Acromegaly Consensus statement (2018). Indeed, prognostic factors and complications were analyzed. Subjects and methods: 40 patients who underwent endoscopic endonasal surgery by acromegaly between 2013 to 2020 was analyzed. Patients were considered in remission if an upper limit of normal (ULN) IGF-1 was less than 1.0 at the six-month and two-year follow-ups. Moreover, we assessed the Knosp grade, tumor volumetry, ULN, T2 signal in MRI, reoperation, and complications. Results: The mean age of admission was 46.7 years. Thirty-two patients were in remission after six months of surgery (80%), decreasing to 76.32% at the two-year follow-up. All microadenomas presented remission (n = 6). Regarding the complications, three patients had permanent panhypopituitarism (7.5%); postoperative cerebrospinal fluid (CSF) leaks did not occur in this series. The hyperintense signal on the T2 MRI and a higher tumor volumetry were the single predictor's factors of non-emission in a multivariate regression logistic analysis (p < 0.05). Preoperative hormone levels (GH and IGF-1) were not a prognostic factor for remission. The re-operated patients who presented hypersignal already had a high predictor of clinical-operative failure. Conclusion: The endoscopic endonasal surgery promotes high short-term and two-year remission rates in acromegaly; the tumor's volumetry and the T2 hypersignal were statistically significant prognostic factors in non-remission – the complications presented at similar rates in comparison to the literature. In invasive GH-secreting tumors, we should offer these patients a multi-disciplinary approach to improve acromegalic patients’ remission rates. Objective shortterm short term twoyear two year followups follow ups th 2018. 2018 . (2018) Indeed analyzed methods 4 201 202 ULN (ULN IGF1 IGF 1 IGF- 10 0 1. sixmonth month followups. ups. Moreover grade T reoperation Results 467 46 7 46. years Thirtytwo Thirty 80%, 80 80% , (80%) 7632 76 32 76.32 followup. followup up. up follow-up n 6. 6 6) 7.5% 75 5 (7.5%) CSF (CSF series predictors s nonemission non emission p 0.05. 005 0.05 05 0.05) GH reoperated re operated clinicaloperative clinical operative failure Conclusion tumors nonremission literature GHsecreting secreting multidisciplinary multi disciplinary (2018 20 8 (80% 763 3 76.3 7.5 (7.5% 00 0.0 (201 2 (80 76. 7. (7.5 0. (20 (8 (7. (2 ( (7

https://doi.org/10.20945/2359-3997000000650

Molecular detection of Toxoplasma gondii, Neospora caninum and Sarcocystis spp in tissues of Sus scrofa slaughtered in southern Brazil gondii
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Freitas, Bibiana Rodrigues de

; Rosa, Gilneia da

; Roman, Isac Junior

; Cunha, Rodrigo Casquero

; Gressler, Letícia Trevisan

; Cargnelutti, Juliana Felipetto

; Vogel, Fernanda Silveira Flôres

Revista Brasileira de Parasitologia Veterinária

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2023, Volume 32 N. 3 elocation e004623

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Resumo O objetivo deste estudo foi determinar a presença de ácido desoxirribonucléico (DNA) de Toxoplasma gondii, Sarcocystis spp. e Neospora caninum, em tecidos de javalis abatidos no sul do Brasil. Foram coletadas 156 amostras de diferentes órgãos de 25 javalis, sendo detectado o DNA de pelo menos um dos protozoários pesquisados em 79 amostras. Para diferenciar entre os agentes infecciosos, o polimorfismo do comprimento do fragmento de restrição, foi realizado usando-se as enzimas de restrição DdeI e HpaII. Para N. caninum, a PCR convencional foi realizada com "primers" específicos. O DNA de pelo menos um dos patógenos estudados foi detectado em cada animal: 26,58% para T. gondii, 68,36% para Sarcocystis spp. e 5,06% para N. caninum. Coinfecção entre T. gondii e Sarcocystis spp. ocorreu em 14 animais; entre T. gondii e N. caninum em apenas um animal macho; entre Sarcocystis spp. e N. caninum em uma fêmea, enquanto a coinfecção com os três agentes foi observada igualmente em apenas um animal macho. Considerando-se a alta frequência de detecção e seu risco zoonótico, especialmente T. gondii, constata-se que os javalis podem ser potenciais fontes de transmissão de agentes infecciosos, e a adoção de medidas de monitoramento nessas populações devem ser priorizadas. (DNA spp Brasil 15 2 7 infecciosos usandose usando se HpaII N primers "primers específicos 2658 26 58 26,58 T 6836 68 36 68,36 506 5 06 5,06 1 animais macho fêmea Considerandose Considerando zoonótico constatase constata priorizadas 265 26,5 683 6 3 68,3 50 0 5,0 26, 68, 5,

Abstract The aim of this study was to determine the presence of deoxyribonucleic acid (DNA) from Toxoplasma gondii, Sarcocystis spp. and Neospora caninum, in tissues of wild boars slaughtered in southern Brazil. A total of 156 samples were collected from different organs of 25 wild boars, and DNA from at least one of the protozoa investigated was detected in 79 samples. To differentiate between infectious agents, restriction fragment length polymorphism was performed using the restriction enzymes DdeI and HpaII. For N. caninum, conventional PCR was performed with specific primers. The DNA of at least one of the studied pathogens was detected in each animal: 26.58% for T. gondii, 68.36% for Sarcocystis spp. and 5.06% for N. caninum. Coinfection between T. gondii and Sarcocystis spp. occurred in 14 animals, between T. gondii and N. caninum in only one male animal, between Sarcocystis spp. and N. caninum in a female, while co-infection with the three agents was equally observed in only one male animal. Considering the high frequency of detection and its zoonotic risk, especially T. gondii, it appears that wild boars can be potential sources of transmission of infectious agents and the adoption of monitoring measures in these populations should be prioritized. (DNA spp Brazil 15 2 7 HpaII N primers animal 2658 26 58 26.58 T 6836 68 36 68.36 506 5 06 5.06 1 animals female coinfection co infection risk prioritized 265 26.5 683 6 3 68.3 50 0 5.0 26. 68. 5.

https://doi.org/10.1590/s1984-29612023048

Association between leukocyte telomere length and sex by quantile regression analysis
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Gutierrez-Rodrigues, Fernanda

; Alves-Paiva, Raquel M.

; Scatena, Natália F.

; Martinez, Edson Z.

; Scheucher, Priscila S.

; Calado, Rodrigo T

Hematology, Transfusion and Cell Therapy

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sep 2022, Volume 44 N. 3 Pages 346 - 351

Abstract: En | Text: En | PDF: En

ABSTRACT Introduction: Telomere length (TL) is a biomarker of cellular proliferative history. In healthy individuals, leukocyte TL shortens with age and associates with the lifespan of men and women. However, most of studies had used linear regression models to address the association of the TL attrition, aging and sex. Methods: We evaluated the association between the TL, aging and sex in a cohort of 180 healthy subjects by quantile regression. The TL of nucleated blood cells was measured by fluorescent in situ hypridization (flow-FISH) in a cohort of 89 men, 81 women, and 10 umbilical cord samples. The results were validated by quantitative polymerase chain reaction (qPCR) and compared to a linear regression analysis. Results: By quantile regression, telomere dynamics slightly differed between sexes with aging: women had longer telomeres at birth and slower attrition rate than men until the sixth decade of life; after that, TL eroded faster and became shorter than that in men. These differences were not observed by linear regression analysis, as the overall telomere attrition rates in women and men were similar (42 pb per year, p < 0.0001 vs. 45 pb kb per year, p < 0.0001). Also, qPCR did not recapitulate flow-FISH findings, as the telomere dynamics by qPCR followed a linear model. Conclusion: The quantile regression analysis accurately reproduced a third-orderpolynomial TL attrition rate in both women and men, but it depended on the technique applied to measure TL. The Flow-FISH reproduced the expected telomere dynamics through life and, differently from the qPCR, was able to detect the subtle TL variations associated with sex and aging.

https://doi.org/10.1016/j.htct.2020.12.005

Distribuição da semiologia do adulto nas escolas médicas do Brasil
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Moniz, Eliane Teixeira Alfama

; Rodrigues, Rodrigo Machado Franco

; Silva Junior, Josué Francisco da

; Grosseman, Suely

Revista Brasileira de Educação Médica

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2022, Volume 46 N. 3 elocation e116

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Resumo: Introdução: A semiologia é a base da prática clínica e seu ensino é essencial no curso de Medicina. Este estudo foi desenvolvido por haver uma lacuna no conhecimento sobre sua distribuição no Brasil. Objetivo: Este estudo teve como objetivo analisar a distribuição da semiologia do adulto nas escolas médicas brasileiras. Método: Trata-se de um estudo transversal descritivo realizado com 226 escolas médicas brasileiras que disponibilizavam a distribuição de semiologia na internet entre as 335 ativas, em dezembro de 2020 (67,5%). As variáveis estudadas foram região geográfica, administração, gratuidade e tempo de existência da escola, carga horária do curso, do internato e de semiologia, e ano(s) ou semestre(s) em que a semiologia era ofertada no currículo. A análise dos dados foi descritiva, e analisaram-se as associações com os testes: t de Student, análise de variância, qui-quadrado de Pearson, U de Mann-Whitney, Kruskal-Wallis e Wilcoxon. Admitiu-se um nível de significância de p < 0,05. Resultado: A semiologia foi mais frequentemente ofertada apenas no quarto semestre (n = 40), seguida por sua oferta em dois semestres: quarto e quinto e terceiro e quarto. Entre as 226 escolas, 142 integravam os conteúdos em módulos ou eixos (62,8%). Entre 117 escolas que forneciam a carga horária de semiologia, mediana foi de 240,0 horas (P25-75 = 165,4 - 338,2), sem diferença estatística por região geográfica, administração, gratuidade e tempo de existência da escola. A mediana da carga teórica de semiologia [77,5 horas (P25-75 = 51,7 - 123,5)] foi menor do que a carga prática [147,0 horas (P25-75 = 64,5 - 180,0)], Z = -3,99, p < 0,01. A mediana da porcentagem da carga horária de semiologia no curso foi de 2,9% (P25-75 = 2,0 - 4,0). Conclusão: A semiologia é mais frequentemente ofertada no quarto semestre, e sua carga horária não difere por características geográficas, de administração e tempo de existência das escolas estudadas.

Abstract: Introduction: Semiology is the basis of clinical practice and its teaching is essential in the medical course. This study was developed to fill a gap in the knowledge about its distribution in Brazil. Objective: To analyze the distribution of adult semiology in Brazilian medical schools. Method: Cross-sectional descriptive study with 226 Brazilian medical schools that provided the distribution of semiology on the Internet among the 335 active schools in December 2020 (67.5%) The variables studied were school geographic region, administration and free tuition, time of existence of the course, course load of the regular course, clerkship and semiology, and year(s) or semester(s) in which semiology was offered in the curriculum. Data analysis was descriptive and the associations were analyzed using: Student’s t, Chi-square, Analysis of Variance, Mann-Whitney-U, Kruskal Wallis and Wilcoxon tests. The significance level was set at p < 0.05. Result: Semiology was more frequently offered only in the 4th semester (n = 40), followed by its offer in two semesters, the 4th and 5th and 3rd and 4th. Among the 226 schools, 142 integrated semiology into modules or axes (62.8%). Among the 117 schools that provided the semiology course load, its median was 240.0 hours (P25-75 = 165.4 - 338.2), with no statistical difference by geographic region, type of administration and time of existence of the course. The median of the theoretical course load [77.5 hours (P25-75 = 51.7 - 123.5)] was lower than the practical course load [147.0 hours (P25-75 = 64.5 - 180.0)], Z = - 3,99, p < 0,01. The median of the percentage of semiology course load during the course was 2.9% (P25-75 = 2.0 - 4.0). Conclusion: Semiology is more frequently offered in the 4th semester and its median course load is similar in Brazilian geographic regions and by school administration type and time of existence of the assessed schools.

https://doi.org/10.1590/1981-5271v46.3-20220106

Memory elicitation, T-cell response and antibody production: an independent study of an inactivated entire virus vaccine (Coronavac)
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SOUZA-SILVA, GUILHERME ANTONIO DE

; AQUINO, RODRIGO CESAR A. DE

; SOUSA, GEORON F. DE

; BARROS, BÁRBARA RAFAELA S.

; LIMA, MARÍLLIA RAPHAELLA C.F. DE

; CRUZ, LEONARDO C.O.

; MARCOS, BIANCA F. SÃO

; SILVA, ANNA JÉSSICA D.

; TALES, APARECIDA VIRGÍNIA S.

; DINIZ, MADI V.

; LIMA, MÔNICA MARIA C.

; SÁ, IGOR W.A. DE

; RODRIGUES, FÁBIO AUGUSTO C.

; SOUZA, EDSON B. DE

; NASCIMENTO, ARIONE V. DO

; VIEIRA, AMANDA M.

; CARVALHO, BRUNO M. DE

; CAMPELO JÚNIOR, EVÔNIO B.

; FREITAS, ANTONIO CARLOS DE

; MELO, CRISTIANE M.L. DE

Anais da Academia Brasileira de Ciências

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2022, Volume 94 N. 3 elocation e20211590

Abstract: En | Text: En | PDF: En

Abstract Health professionals working to mitigate the COVID-19 pandemic are one of the main risk groups for the disease, being prioritized for vaccination. Considering this, the aim of this study was to analyze the immune response of these professionals immunized with CoronaVac in the first and second doses. Blood samples were collected after the first and second doses of the vaccine (CoronaVac) and used to investigate hematological and biochemical parameters, analysis of immunoglobulin production, cytokines, and gene expression profile, as well as the identification of subsets of immune cells. Post-first dose immunological phenotypic memory (CD27+) profiles (T CD4+, TCD8+ and CD19+) showed a significant increase, as did Monocyte APCs (CD80+HLA-DR+) in relation to the second dose. The cytokines IL-2, IL-6 and IFN-° showed increased values in relation to the other analyzed cytokines. The Th2/Th17 profile in the second dose was characterized by gene expression analysis. The production of IgM and IgG after vaccination showed statistically significant values in the comparison between doses. CoronaVac showed activation of APCs monocytes, memory response of T and B lymphocytes, with immunoglobulins production. This set of responses is characterized by the Th2/Th17 immunological profile.

https://doi.org/10.1590/0001-3765202220211590

Association between handgrip strength and bone mass parameters in HIV-infected children and adolescents. A cross-sectional study
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Martins, Priscila Custódio

; de Lima, Luiz Rodrigo Augustemak

; de Lima, Tiago Rodrigues

; Petroski, Edio Luiz

; Silva, Diego Augusto Santos

Sao Paulo Medical Journal

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aug 2021, Volume 139 N. 4 Pages 405 - 411

Abstract: En | Text: En | PDF: En

ABSTRACT BACKGROUND: Low bone mineral content (BMC) and bone mineral density (BMD) have been identified in human immunodeficiency virus (HIV)-infected children and adolescents. The direct adverse effects of HIV infection and combined antiretroviral therapy (ART) negatively contribute to bone metabolism. A direct relationship between muscle strength levels and BMD in HIV-infected adults and older adults has been described. However, it is unknown whether handgrip strength (HGS) is associated with bone mass in pediatric populations diagnosed with HIV. OBJECTIVE: To ascertain whether HGS levels are associated with BMC and BMD in HIV-infected children and adolescents. DESIGN AND SETTING: Cross-sectional study conducted in Florianãpolis, Brazil, in 2016. METHODS: The subjects were 65 children and adolescents (8-15 years) diagnosed with vertically-transmitted HIV. Subtotal and lumbar-spine BMC and BMD were obtained via dual-emission X-ray absorptiometry (DXA). HGS was measured using manual dynamometers. The covariates of sex, ART, CD4+ T lymphocytes and viral load were obtained through questionnaires and medical records. Sexual maturation was self-reported and physical activity was measured using accelerometers. Simple and multiple linear regression were used, with P < 0.05. RESULTS: HGS was directly associated with subtotal BMD (β = 0.002; R² = 0.670; P < 0.001), subtotal BMC (β = 0.090; R² = 0.734; P = 0.005) and lumbar-spine BMC (β = 1.004; R² = 0.656; P = 0.010) in the adjusted analyses. However, no significant association was found between HGS and lumbar-spine BMD (β = 0.001; R² = 0.464; P = 0.299). CONCLUSION: HGS was directly associated with BMD and BMC in HIV-infected children and adolescents.

https://doi.org/10.1590/1516-3180.2020.0539.r1.090321 4 downloads

10.

Avaliação morfofuncional do corpo lúteo para diagnóstico precoce de gestação 20 dias após IATF em vacas mestiças leiteiras
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Silva, Mariana A.A.

; Rodrigues, Alexandra S.

; Brandão, Tiago O.

; Maggitti Junior, Luiz Di Paolo

; Carôso, Bia S.S.

; Chalhoub, Marcos

; Bittencourt, Rodrigo F.

; Ribeiro Filho, Antonio L.

Pesquisa Veterinária Brasileira

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oct 2018, Volume 38 N. 10 Pages 2006 - 2011

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO: A detecção superprecoce de gestação é de grande relevância quando se trata do aumento da produtividade dos rebanhos, na tentativa de redução do tempo de serviço desses animais. Por tal relevância, objetivou-se utilizar a ultrassonografia (US) Doppler como ferramenta para predição de gestação, avaliando a irrigação do corpo lúteo (CL), vinte dias após IATF. Setenta e três vacas mestiças foram avaliadas 20 dias após IATF por US transretal. Para obtenção do diâmetro do CL (DCL) e sua área (ACL) utilizou-se o modo B, na mensuração da área de vascularização (VCL) e seu percentual no CL (VCL%) empregou-se o Doppler Colorido. As imagens foram classificadas com base na quantidade de pixels coloridos e sua distribuição no CL, em dois grupos: diagnóstico preditivo negativo de gestação (DPN) e preditivo positivo (DPP). No mesmo dia, a funcionalidade do CL foi determinada pela concentração plasmática de P4. O diagnóstico de gestação definitivo foi realizado por US 35 dias após as IATFs. Utilizou-se o teste t de Student (com P<0,05) na avaliação das variáveis. Grupo gestantes apresentou médias superiores (P<0,05) para todas as variáveis ao Grupo Não Gestantes. O diagnóstico preditivo demonstrou 83,33% de especificidade, sensibilidade de 100% e acurácia de 91,79%. Conclui-se que a US doppler do CL aos 20 dias pós IATF é um método confiável no diagnóstico precoce de gestação.

ABSTRACT: The super early gestation detection is of great relevance considering the increase of the herds’ productivity, in an attempt to reduce the time of service of these animals. Due to this relevance, this study aimed to use Doppler ultrasonography as a tool for gestation prediction, evaluating the luteal body (CL) irrigation twenty days after FTAI. 73 crossbred cows were evaluated 20 days after FTAI through trans rectal US. In order to obtain the diameter of the CL (DCL) and its area (ACL), B mode was used. The Color Doppler was applied to measure the vascularization area (CLV) and its percentage in the CL (VCL %). The images were classified, based on the number of colored pixels and their distribution in CL, in two groups: negative predictive diagnosis of gestation (NPD) and positive predictive value (PPV). On the same day, LC functionality was determined based on P4 plasma concentration. The definitive gestation diagnosis was confirmed through US 35 days after the FTAIs. Student’s T test (with P<0.05) was used to evaluate the variables. Pregnant group presented higher averages (P<0.05) for all variables compared to the Non-Pregnant Group. The predictive diagnosis showed 83.33% of specificity, 100% of sensitivity and 91.79% of accuracy. The conclusion is that the US Doppler at 20 days post FTAI is a reliable method for early gestation diagnosis.

https://doi.org/10.1590/1678-5150-pvb-5413 1966 downloads

11.

Management Systems Effect on Fertility Indicators of a Ferralsol with Vegetable Crops, as Determined by Different Statistical Tools
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Lima, Carlos Eduardo Pacheco

; Silva, Juscimar da

; Guedes, Ítalo Moraes Rocha

; Madeira, Nuno Rodrigo

; Fontenelle, Mariana Rodrigues

Revista Brasileira de Ciência do Solo

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2017, Volume 41 elocation e0160468

Abstract: En | Text: En | PDF: En

ABSTRACT The intensive nature of soil use in vegetable production areas has led to a marked decrease in soil quality. The objective of this study was to evaluate the effects of adoption of soil management systems on vegetable production with regards to the chemical properties of a Rhodic Ferralsol after five years and to evaluate the use of the Principal Component Analysis (PCA) statistical tool in discriminating the different treatments. The experiment was conducted under field conditions in central Brazil in a randomized block design with four replications and a 3 × 2 factorial arrangement (three soil management systems × two cover crops). The soil management systems used were NT (no-tillage), RT (reduced tillage), and CT (conventional tillage). The cover crops used were corn (Zea mays) alone and corn intercropped with the gray velvet bean (Stizolobium niveum). Reduced tillage showed the highest values of sum of bases, cation exchange capacity (T), and total organic carbon contents in the 0.00-0.05 m layer. In the same layer, RT and CT showed higher values of pH and K content. No-tillage and RT showed the highest P and Ca2+ contents and H+Al and T values. In the 0.05-0.10 m layer, RT had higher a pH value and Mg2+ contents. No-tillage and CT had higher potential acidity in this layer. The management systems (0.10-0.30 m) and the cover plants (all layers) had no effect on the properties analyzed. The use of PCA determined that the two principal components explained the following percentage of the data variance: 90.8 % (0.0-0.05 m), 79.8 % (0.05-0.10 m), and 83.1 % (0.10-0.30 m). Analysis of the eigenvectors and the grouping of treatments in PCA also showed that RT was most effective in improving soil fertility properties. Reduced tillage was most effective in increasing soil fertility after five years. The PCA is recommended as a useful tool and it allowed the identification of patterns not revealed by traditional tools.

https://doi.org/10.1590/18069657rbcs20160468 603 downloads

12.

ERICA: prevalence of metabolic syndrome in Brazilian adolescents
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Kuschnir, Maria Cristina C

; Bloch, Katia Vergetti

; Szklo, Moyses

; Klein, Carlos Henrique

; Barufaldi, Laura Augusta

; Abreu, Gabriela de Azevedo

; Schaan, Beatriz

; Veiga, Gloria Valeria da

; Silva, Thiago Luiz Nogueira da

; Vasconcellos, Maurício T L de

; Moraes, Ana Júlia Pantoja de

; Oliveira, Ana Mayra Andrade de

; Tavares, Bruno Mendes

; Oliveira, Cecília Lacroix de

; Cunha, Cristiane de Freitas

; Giannini, Denise Tavares

; Belfort, Dilson Rodrigues

; Santos, Eduardo Lima

; Leon, Elisa Brosina de

; Oliveira, Elizabete Regina Araújo

; Fujimori, Elizabeth

; Borges, Ana Luíza

; Magliano, Erika da Silva

; Vasconcelos, Francisco de Assis Guedes

; Azevedo, George Dantas

; Brunken, Gisela Soares

; Guimarães, Isabel Cristina Britto

; Faria Neto, José Rocha

; Oliveira, Juliana Souza

; Carvalho, Kenia Mara B de

; Gonçalves, Luis Gonzaga de Oliveira

; Monteiro, Maria Inês

; Santos, Marize M

; Muniz, Pascoal Torres

; Jardim, Paulo César B Veiga

; Ferreira, Pedro Antônio Muniz

; Montenegro Jr, Renan Magalhães

; Gurgel, Ricardo Queiroz

; Vianna, Rodrigo Pinheiro

; Vasconcelos, Sandra Mary

; Martins, Stella Maris Seixas

; Goldberg, Tamara Beres Lederer

Revista de Saúde Pública

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2016, Volume 50 Suppl. 1 elocation 11s

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO OBJETIVO Determinar a prevalência de síndrome metabólica e de seus componentes em adolescentes brasileiros. MÉTODOS Foram avaliados 37.504 adolescentes, participantes do Estudo de Riscos Cardiovasculares em Adolescentes (ERICA), estudo transversal de âmbito nacional e de base escolar. Os adolescentes, de 12 a 17 anos de idade, residiam em municípios com mais de 100 mil habitantes. A amostra foi estratificada e conglomerada em escolas e turmas. Os critérios da International Diabetes Federation foram utilizados para definir síndrome metabólica. Prevalências de síndrome metabólica foram estimadas segundo sexo, faixa etária, tipo de escola e estado nutricional. RESULTADOS Dos 37.504 adolescentes, 50,2% eram do sexo feminino; 54,3% tinham de 15 a 17 anos e 73,3% estudavam em escolas públicas. A prevalência nacional de síndrome metabólica foi 2,6% (IC95% 2,3-2,9), discretamente maior no sexo masculino e naqueles de 15 a 17 anos na maioria das macrorregiões. A prevalência foi a maior nos residentes na macrorregião Sul, nas adolescentes mais jovens e nos adolescentes mais velhos. A prevalência foi maior nas escolas públicas (2,8% [IC95% 2,4-3,2]) que nas escolas privadas (1,9% [IC95% 1,4-2,4]) e nos adolescentes obesos em comparação aos não obesos. As combinações de componentes mais frequentes, respondendo por 3/4 das combinações, foram: circunferência de cintura elevada, HDL-colesterol baixo e pressão arterial elevada, seguida de circunferência da cintura elevada, lipoproteína de alta densidade (HDL-c) baixo e triglicerídeos elevados, e wpor circunferência da cintura elevada, HDL baixa e triacilgliceróis e pressão arterial elevados. A HDL baixa foi o segundo componente mais frequente, mas a maior prevalência de síndrome metabólica (26,8%) foi observada na presença de triglicerídeos elevado. CONCLUSÕES O ERICA é o primeiro estudo nacional a apresentar prevalências de síndrome metabólica e descrever a participação dos seus componentes. Apesar de a prevalência da síndrome metabólica ter sido baixa, as altas prevalências de alguns componentes e de participação de outros na composição da síndrome torna importante o diagnóstico precoce de tais alterações, mesmo que não agrupadas na síndrome metabólica.

ABSTRACT OBJECTIVE To determine the prevalence of metabolic syndrome and its components in Brazilian adolescents. METHODS We evaluated 37,504 adolescents who were participants in the Study of Cardiovascular Risks in Adolescents (ERICA), a cross-sectional, school-based, national study. The adolescents, aged from 12 to 17 years, lived in cities with populations greater than 100,000 inhabitants. The sample was stratified and clustered into schools and classes. The criteria set out by the International Diabetes Federation were used to define metabolic syndrome. Prevalences of metabolic syndrome were estimated according to sex, age group, school type and nutritional status. RESULTS Of the 37,504 adolescents who were evaluated: 50.2% were female; 54.3% were aged from 15 to 17 years, and 73.3% were from public schools. The prevalence of metabolic syndrome was 2.6% (95%CI 2.3-2.9), slightly higher in males and in those aged from 15 to 17 years in most macro-regions. The prevalence was the highest in residents from the South macro-region, in the younger female adolescents and in the older male adolescents. The prevalence was higher in public schools (2.8% [95%CI 2.4-3.2]), when compared with private schools (1.9% [95%CI 1.4-2.4]) and higher in obese adolescents when compared with nonobese ones. The most common combinations of components, referring to 3/4 of combinations, were: enlarged waist circumference (WC), low HDL-cholesterol (HDL-c) and high blood pressure; followed by enlarged WC, low HDL-c and high triglycerides; and enlarged WC, low HDL-c, high triglycerides and blood pressure. Low HDL was the second most frequent component, but the highest prevalence of metabolic syndrome (26.8%) was observed in the presence of high triglycerides. CONCLUSIONS ERICA is the first Brazilian nation-wide study to present the prevalence of metabolic syndrome and describe the role of its components. Despite the prevalence of Metabolic Syndrome being low, the high prevalences of some components and participation of others in the syndrome composition shows the importance of early diagnosis of this changes, even if not grouped within the metabolic syndrome.

https://doi.org/10.1590/s01518-8787.2016050006701 1789 downloads

13.

ERICA: prevalence of metabolic syndrome in Brazilian adolescents
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Kuschnir, Maria Cristina C

; Bloch, Katia Vergetti

; Szklo, Moyses

; Klein, Carlos Henrique

; Barufaldi, Laura Augusta

; Abreu, Gabriela de Azevedo

; Schaan, Beatriz

; Veiga, Gloria Valeria da

; Silva, Thiago Luiz Nogueira da

; Vasconcellos, Maurício T L de

; Moraes, Ana Júlia Pantoja de

; Oliveira, Ana Mayra Andrade de

; Tavares, Bruno Mendes

; Oliveira, Cecília Lacroix de

; Cunha, Cristiane de Freitas

; Giannini, Denise Tavares

; Belfort, Dilson Rodrigues

; Santos, Eduardo Lima

; Leon, Elisa Brosina de

; Oliveira, Elizabete Regina Araújo

; Fujimori, Elizabeth

; Borges, Ana Luíza

; Magliano, Erika da Silva

; Vasconcelos, Francisco de Assis Guedes

; Azevedo, George Dantas

; Brunken, Gisela Soares

; Guimarães, Isabel Cristina Britto

; Faria Neto, José Rocha

; Oliveira, Juliana Souza

; Carvalho, Kenia Mara B de

; Gonçalves, Luis Gonzaga de Oliveira

; Monteiro, Maria Inês

; Santos, Marize M

; Muniz, Pascoal Torres

; Jardim, Paulo César B Veiga

; Ferreira, Pedro Antônio Muniz

; Montenegro Jr, Renan Magalhães

; Gurgel, Ricardo Queiroz

; Vianna, Rodrigo Pinheiro

; Vasconcelos, Sandra Mary

; Martins, Stella Maris Seixas

; Goldberg, Tamara Beres Lederer

Revista de Saúde Pública

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2016, Volume 50 Suppl. 1 elocation 11s

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

https://doi.org/10.1590/s01518-8787.2016050006701 11875 downloads

14.

Comparative study of lymphocytes from individuals that were vaccinated and unvaccinated against the pandemic 2009-2011 H1N1 influenza virus in Southern Brazil
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Freitas, Deise Nascimento de

; Isaía, Henrique Ataíde

; Henzel, Andréia

; Simão, Eder

; Gassen, Rodrigo Benedetti

; Rodrigues Junior, Luiz Carlos

Revista da Sociedade Brasileira de Medicina Tropical

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oct 2015, Volume 48 N. 5 Pages 514 - 523

Abstract: En | Text: En | PDF: En

ABSTRACTINTRODUCTION:While no single factor is sufficient to guarantee the success of influenza vaccine programs, knowledge of the levels of immunity in local populations is critical. Here, we analyzed influenza immunity in a population from Southern Brazil, a region with weather conditions that are distinct from those in the rest of country, where influenza infections are endemic, and where greater than 50% of the population is vaccinated annually.METHODS:Peripheral blood mononuclear cells were isolated from 40 individuals. Of these, 20 had received the H1N1 vaccine, while the remaining 20 were unvaccinated against the disease. Cells were stimulated in vitro with the trivalent post-pandemic influenza vaccine or with conserved major histocompatibility complex I (MHC I) peptides derived from hemagglutinin and neuraminidase. Cell viability was then analyzed by [3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide)]-based colorimetric assay (MTT), and culture supernatants were assayed for helper T type 1 (Th1) and Th2-specific cytokine levels.RESULTS:Peripheral blood lymphocytes from vaccinated, but not unvaccinated, individuals exhibited significant proliferation in vitro in the presence of a cognate influenza antigen. After culturing with vaccine antigens, cells from vaccinated individuals produced similar levels of interleukin (IL)-10 and interferon (IFN)-γ, while those from unvaccinated individuals produced higher levels of IFN-γ than of IL-10.CONCLUSIONS:Our data indicate that peripheral blood lymphocytes from vaccinated individuals are stimulated upon encountering a cognate antigen, but did not support the hypothesis that cross-reactive responses related to previous infections can ameliorate the immune response. Moreover, monitoring IL-10 production in vaccinated individuals could comprise a valuable tool for predicting disease evolution.

https://doi.org/10.1590/0037-8682-0163-2015 2773 downloads

15.

Posição habitual da língua e dos lábios nos padrões de crescimento anteroposterior e vertical
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Berwig, Luana Cristina

; Ritzel, Rodrigo Agne

; Silva, Ana Maria Toniolo da

; Mezzomo, Carolina Lisbôa

; Côrrea, Eliane Castilhos Rodrigues

; Serpa, Eliane Oliveira

Revista CEFAC

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mar 2015, Volume 17 Suppl. 1 Pages 107 - 114

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

OBJETIVO: estudar a posição habitual da língua e dos lábios nos padrões de crescimento anteroposterior e vertical de crianças em fase de dentição mista. MÉTODOS: a amostra foi constituída por 54 crianças, na faixa etária entre sete e 11 anos. As crianças selecionadas foram encaminhadas para avaliação radiográfica e análise cefalométrica, que possibilitou a obtenção de ângulos SNA, SNB e ANB (padrão de crescimento anteroposterior) e da classificação do tipo facial entre braquifacial, mesofacial e dolicofacial (padrão de crescimento vertical). A posição da língua e dos lábios foi determinada a partir da observação das radiografias cefalométricas por duas fonoaudiólogas com experiência na área de motricidade orofacial. A posição habitual da língua foi classificada como na papila palatina, com dorso elevado ou no assoalho oral, e a posição habitual dos lábios, como fechados ou entreabertos/abertos. Para verificar a relação entre a posição habitual da língua e dos lábios com os padrões de crescimento anteroposterior e vertical foram utilizados os testes estatísticos Análise de Variância, t de Student, U de Mann-Whitney e Qui-Quadrado, ao nível de significância de 5%. RESULTADOS: verificou-se relação estatisticamente significante entre a posição da língua e o ângulo SNB, sendo que as crianças com posição de língua no assoalho oral apresentaram ângulo SNB significantemente menor do que as crianças com posição habitual de língua na papila palatina. Verificou-se ângulo SNB significantemente menor nas crianças com posição de lábios entreabetos ou abertos quando comparadas às crianças com posição de lábios fechados. Não houve diferença entre a posição habitual da língua e dos lábios nos demais padrões de crescimento anteroposterior e de crescimento vertical. CONCLUSÃO: a posição habitual de lábios e de língua apresentou relação com o padrão de crescimento mandibular, não tendo sido influenciada pelo tipo facial.

PURPOSE: to study the usual tongue and lips position in anteroposterior and vertical growth patterns in children with mixed dentition. METHODS: the sample comprised 54 children, aged seven to 11 years old. The selected children were referred for radiographic evaluation and cephalometric analysis, which made it possible to obtain the SNA, SNB and AND angles (anteroposterior growth pattern) and the classification of the facial type: brachyfacial, mesofacial and dilocofacial (vertical growth pattern). The tongue and lips position was determined from the observation of cephalometric radiographs made by two speech therapists experienced in orofacial motricity. The usual tongue position was classified as in the papilla, high dorsum or on the floor of the mouth, and the usual lips position, as closed or half-open/open. In order to verify the relationship between the usual tongue and lips position with anteroposterior and vertical growth patterns, statistical tests like Analysis of variance, Student's t test, Mann-Whitney U and chi-square test at a significance level of 5% was used. RESULTS: a statistically significant relationship between the tongue position and the SNB angle was identified, children with tongue position on the floor of the mouth showed significantly lower SNB angle than children with tongue position in the papilla. SNB angle was a statistically significant lower in children with open or half open lips than children with closed lips. There was no difference between the normal position of the tongue and lips in other growth patterns anteroposterior and vertical growth. CONCLUSION: The usual position of lips and tongue were related to mandibular growth pattern and hasn't been influenced by facial type.

https://doi.org/10.1590/1982-0216201517s112112 4436 downloads

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