ABSTRACT OBJECTIVE: To describe disability-related performance and inequality nationwide in Brazil, and the changes that took place between 2012 and 2019 after the introduction of Programme for Improving Primary Care Access and Quality (PMAQ). METHODS: We derived scores for disability-related care and accessibility of primary healthcare facilities from PMAQ indicators collected in round 1 (2011–2013), and round 3 (2015–2019). We assessed how scores changed after the introduction of PMAQ. We used census data on per capita income of local areas to examine the disability-specific care and accessibility scores by income group. We undertook ordinary least squares regressions to examine the association between PMAQ scores and per capita income of each local area across implementation rounds. RESULTS: Disability-related care scores were low in round 1 (18.8, 95%CI 18.3–19.3, out of a possible 100) and improved slightly by round 3 (22.5, 95%CI 22.0–23.1). Accessibility of primary healthcare facilities was also poor in round 1 (30.3, 95%CI 29.8–30.8) but doubled by round 3 (60.8, 95%CI 60.3–61.3). There were large socioeconomic inequalities in round 1, with both scores approximately twice as high in the richest compared to the poorest group. Inequalities weakened somewhat for accessibility scores by round 3. These trends were confirmed through regression analyses, controlling for other area characteristics. Disability-related and accessibility scores also varied strongly between states in both rounds. CONCLUSIONS: People with disabilities are being left behind by the Brazilian healthcare system, particularly in poor areas, which will challenge the achievement of universal health coverage.

ABSTRACT OBJECTIVE: To describe disability-related performance and inequality nationwide in Brazil, and the changes that took place between 2012 and 2019 after the introduction of Programme for Improving Primary Care Access and Quality (PMAQ). METHODS: We derived scores for disability-related care and accessibility of primary healthcare facilities from PMAQ indicators collected in round 1 (2011–2013), and round 3 (2015–2019). We assessed how scores changed after the introduction of PMAQ. We used census data on per capita income of local areas to examine the disability-specific care and accessibility scores by income group. We undertook ordinary least squares regressions to examine the association between PMAQ scores and per capita income of each local area across implementation rounds. RESULTS: Disability-related care scores were low in round 1 (18.8, 95%CI 18.3–19.3, out of a possible 100) and improved slightly by round 3 (22.5, 95%CI 22.0–23.1). Accessibility of primary healthcare facilities was also poor in round 1 (30.3, 95%CI 29.8–30.8) but doubled by round 3 (60.8, 95%CI 60.3–61.3). There were large socioeconomic inequalities in round 1, with both scores approximately twice as high in the richest compared to the poorest group. Inequalities weakened somewhat for accessibility scores by round 3. These trends were confirmed through regression analyses, controlling for other area characteristics. Disability-related and accessibility scores also varied strongly between states in both rounds. CONCLUSIONS: People with disabilities are being left behind by the Brazilian healthcare system, particularly in poor areas, which will challenge the achievement of universal health coverage. OBJECTIVE disabilityrelated disability related Brazil 201 . (PMAQ) METHODS 2011–2013, 20112013 2011–2013 , 2011 2013 (2011–2013) 2015–2019. 20152019 2015–2019 2015 (2015–2019) disabilityspecific specific group rounds RESULTS Disabilityrelated Disability 18.8, 188 18 8 (18.8 95CI CI 95 183193 19 18.3–19.3 100 22.5, 225 22 5 (22.5 22.0–23.1. 220231 22.0–23.1 0 23 22.0–23.1) 30.3, 303 30 (30.3 29.8–30.8 298308 29 60.8, 608 60 (60.8 60.3–61.3. 603613 60.3–61.3 61 60.3–61.3) analyses characteristics CONCLUSIONS system coverage 20 (PMAQ 2011201 2011–201 (2011–2013 2015201 2015–201 (2015–2019 18.8 (18. 9 18319 18.3–19. 10 22.5 2 (22. 22023 22.0–23. 30.3 (30. 29.8–30. 29830 60.8 6 (60. 60361 60.3–61. 201120 2011–20 (2011–201 201520 2015–20 (2015–201 18. (18 1831 18.3–19 22. (22 2202 22.0–23 30. (30 29.8–30 2983 60. (60 6036 60.3–61 20112 2011–2 (2011–20 20152 2015–2 (2015–20 (1 183 18.3–1 (2 220 22.0–2 (3 29.8–3 298 (6 603 60.3–6 2011– (2011–2 2015– (2015–2 ( 18.3– 22.0– 29.8– 60.3– (2011– (2015– 18.3 22.0 29.8 60.3 (2011 (2015 29. (201 (20

Resumo O Processo Transexualizador é o principal serviço direcionado à saúde trans-específica no Brasil, o qual oferta aquisições como hormonioterapia e cirurgias de redesignação sexual à população trans. Neste artigo, analisaram-se os sentidos produzidos em torno do procedimento cirúrgico, da nova genitália, sobre gênero e transexualidade entre mulheres que realizaram cirurgia de redesignação sexual no Hospital Universitário Cassiano Antônio de Moraes (HUCAM), localizado em Vitória, Estado do Espírito Santo, Brasil. Foi realizada pesquisa qualitativa com entrevistas narrativas gravadas em áudio, e realizada análise de conteúdo. Foram selecionadas 09 participantes a partir da metodologia bola de neve. Conclui-se que as cirurgias estão relacionadas à busca pela humanização dos corpos. As compreensões sobre ser mulher não se limitaram a um estatuto biológico para os gêneros. A transexualidade foi apresentada como uma experiência de transição entre os gêneros. A cirurgia de redesignação sexual foi apontada como estratégia para a construção de um corpo em consonância com a experiência das participantes nas relações sociais de gênero, cujo sucesso é avaliado sob signos de beleza, prazer e passabilidade.

Abstract The Transsexualization Process is the main service directed to trans-specific health in Brazil, which provides services such as hormone therapy and sexual reassignment surgeries to the trans population. In this article, the significance surrounding the surgical procedure, the new genitalia, gender and transsexuality among women who underwent sexual reassignment surgery at the Cassiano Antônio de Moraes University Hospital (HUCAM), located in Vitória, Espírito Santo State, Brazil, was analyzed. Qualitative research was carried out, narrative interviews were recorded, and thematic content analysis was performed. The sample was composed of 9 participants selected by snowball sampling. The conclusion drawn is that the surgeries are related to the search for the humanization of bodies. The comprehension about being a woman was not limited to a biological status for gender. Transsexuality was presented as a transitional experience between genders. The sexual reassignment surgery was seen as a strategy of corporal correction in line with expectations about social relationships in gender by participants, the success of which is evaluated from the standpoint of beauty, pleasure and passability.

Abstract INTRODUCTION: The Amazon tropical rainforest has the most dense and diverse ecosystem worldwide. A few studies have addressed rodent-borne diseases as potential hazards to humans in this region. METHODS: A retrospective survey was conducted using enzyme-linked immunosorbent assay for detecting mammarenavirus and orthohantavirus antibodies in 206 samples collected from rural settlers of the Brazilian Western Amazonian region. RESULTS: Six (2.91%) individuals in the age group of 16 to 36 years were found to possess antibodies against mammarenavirus. CONCLUSION: Evidence of previous exposure to mammarenavirus in the rural population points to its silent circulation in this region.

Resumo O Processo Transexualizador é o principal serviço direcionado à saúde trans-específica no Brasil, o qual oferta aquisições como hormonioterapia e cirurgias de redesignação sexual à população trans. Neste artigo, analisaram-se os sentidos produzidos em torno do procedimento cirúrgico, da nova genitália, sobre gênero e transexualidade entre mulheres que realizaram cirurgia de redesignação sexual no Hospital Universitário Cassiano Antônio de Moraes (HUCAM), localizado em Vitória, Estado do Espírito Santo, Brasil. Foi realizada pesquisa qualitativa com entrevistas narrativas gravadas em áudio, e realizada análise de conteúdo. Foram selecionadas 09 participantes a partir da metodologia bola de neve. Conclui-se que as cirurgias estão relacionadas à busca pela humanização dos corpos. As compreensões sobre ser mulher não se limitaram a um estatuto biológico para os gêneros. A transexualidade foi apresentada como uma experiência de transição entre os gêneros. A cirurgia de redesignação sexual foi apontada como estratégia para a construção de um corpo em consonância com a experiência das participantes nas relações sociais de gênero, cujo sucesso é avaliado sob signos de beleza, prazer e passabilidade.

Abstract The Transsexualization Process is the main service directed to trans-specific health in Brazil, which provides services such as hormone therapy and sexual reassignment surgeries to the trans population. In this article, the significance surrounding the surgical procedure, the new genitalia, gender and transsexuality among women who underwent sexual reassignment surgery at the Cassiano Antônio de Moraes University Hospital (HUCAM), located in Vitória, Espírito Santo State, Brazil, was analyzed. Qualitative research was carried out, narrative interviews were recorded, and thematic content analysis was performed. The sample was composed of 9 participants selected by snowball sampling. The conclusion drawn is that the surgeries are related to the search for the humanization of bodies. The comprehension about being a woman was not limited to a biological status for gender. Transsexuality was presented as a transitional experience between genders. The sexual reassignment surgery was seen as a strategy of corporal correction in line with expectations about social relationships in gender by participants, the success of which is evaluated from the standpoint of beauty, pleasure and passability.

ABSTRACT During the Brazilian slavery period, many African migrants were brought to the American continent. Historically, some of these migrants escaped from the Brazilian gold mines and farms to which they had been brought and settled in remote valleys and this was the main mode of resistance to the slavery system. These runaway-slave descendant communities are called quilombos, a group with distinct ethnic identity, specific behavioral habits, including geographic isolation and conservative practices. The objective of this study was to investigate the prevalence of rodent-borne viruses in two Afro-descendent communities from Mato Grosso do Sul State, Midwestern Brazil. A total of 319 individuals from rural and urban quilombola communities were enrolled. Twelve (3.76%) had anti-rodent-borne virus IgG antibodies. Seven (2.19%) were anti-mammarenavirus reactive and nine (2.82%) had anti-orthohantavirus antibodies. The literature includes limited data on the health status of quilombola communities, but all the studies emphasize the disparity of attention of local healthcare personnel to these communities compared to the general population. The findings of this study highlight the vulnerability and the precarious health conditions of quilombola groups, especially those living in rural areas and thus, point to the need of preventive measures to improve access to healthcare for this ethnic group.

Resumo Este trabalho teve como objetivo catalogar as espécies de répteis da Bacia Hidrográfica do Rio Sinos com base em registros de coleções científicas e dados coletados em campo. Registramos 65 espécies, incluindo 46 serpentes, nove lagartos, cinco tartarugas, quatro anfisbenas e um jacaré. As serpentes compuseram a maioria dos espécimes registrados (91,3%), e as três espécies mais representativas são peçonhentas e de importância médica. A região mais urbana da bacia (planície) possui o maior número de registros. Este fato pode ser um reflexo da alta densidade populacional humana nesta região, o que teria favorecido o encontro de espécimes e seu envio para coleções científicas e centros de pesquisa. Vale ressaltar que a maioria das espécies com poucos exemplares nas coleções também são raramente observadas na natureza, como Clelia hussani e Urostrophus vautieri. Esta observação torna viável que essas populações sejam pequenas ou que estejam em declínio.

Abstract This work aimed to catalog the species of reptiles of the Sinos River Basin based on records from scientific collections and data collected in the field. We recorded 65 species, including 46 snakes, nine lizards, five turtles, four amphisbaenians and one caiman. Snakes composed most of the recorded specimens (91.3%), and the three most representative are venomous and of medical importance. The most urban region of the basin (Lowland) has the highest number of records. This fact may be a reflection of the high human population density in this region, which would have favored the encounter of specimens and their sending to scientific collections and research centers. It is worth highlighting that most species with few specimens in the collections are also rarely observed in the wild, such as Clelia hussani and Urostrophus vautieri. This observation makes it feasible that these populations are small or that they are declining.

BACKGROUND Human herpesvirus 2 (HHV-2) have DNA genome with a limited genetic variability and have been classified into two clades. OBJECTIVES To identify and characterise six HHV-2 isolates derived from Brazilian women. METHODS HHV-2 isolates were performed polymerase chain reaction (PCR) and sequencing of 2250 pb of the glycoprotein B (gB) coding regions. FINDINGS Four HHV-2 isolates were classified into clade B, while the remaining two, derived from HIV-1 co-infected women, showed a notable genetic divergence (> 1%). MAIN CONCLUSION The results reveal novel HHV-2 variants. The impact of these novel variants on HHV-2 pathogenesis and HIV/HHV-2 coinfection need to be investigated.

AbstractEvening primrose (Oenothera biennis L., Onagraceae) seeds oil has great economic importance due to its wide industrial application, mainly for medicines and nutraceutics. However, to our knowledge, it remains almost unexplored regarding development of innovative formulations, such as nanoemulsions. On the present study, required Hydroprophile–Lipophile Balance of evening primrose seeds oil was determined (HLB 12) and a stable nanoemulsion (Day 1: mean droplet size: 214.3 ± 0.69 nm, polydispersity index: 0.253 ± 0.012. Day 7: mean droplet size: 202.8 ± 0.23 nm, polydispersity index: 0.231 ± 0.008) was achieved. Moreover, pseudo-ternary diagram allowed delimitation of nanoemulsion region, contributing to nanobiotechnology of natural products.

Copaiba (Copaifera duckei Dwyer, Fabaceae) oleoresin is an important Amazonian raw material. Despite its insecticidal potential, poor water solubility remains a challenge for the development of effective and viable products. Nanotechnology has emerged as a promising area to solve this problem, especially oil-in-water nanoemulsions. On this context, the aim of the present study was to develop oil-in-water nanoemulsions using copaiba oleoresin dispersed through a high internal phase; and evaluate its potential insecticidal action against Aedes aegypti larvae. Overall, 31 formulations were prepared, ranging from 11.5 ± 0.2 to 257.3 ± 4.1 nm after one day of manipulation. Some of them reached small mean droplet sizes (< 200 nm) and allowed achievement of a nanoemulsion region. The formulation consisted of 5% (w/w) of copaiba oil, 5% (w/w) of surfactant and 90% (w/w) of water, which presented mean droplet size of 145.2 ±0.9 nm and polidispersity of 0.378 ± 0.009 after one day of manipulation, and these were evaluated for larvicidal potential. According to mortality level (250 ppm - 93.3 after 48 h), this nanoemulsion was classified as a promising insecticidal agent against Aedes aegypti larvae. The present study allowed the development of low-cost ecofriendly green natural-based nanoformulations with potential larvicidal activity, using a nanobiotechnology approach.

Introduction Over the last recent years, the number of Q fever cases have has increased throughout the world. An epidemiological investigation was performed in the area in which the first molecular documentation of Q fever in Brazil was previously reported. Methods Indirect immunofluorescence assay (IFA) and PCR of Coxiella burnetii targeting the htpAB gene were performed in samples from 14 dogs (blood); 1 cat (blood); 10 goats (blood, milk, vaginal swab and anal swab); 3 sheep (blood); and 2 horses (blood). Results Two dogs, two sheep and five goats were seroreactive. DNA was amplified from 6 milk and 2 blood samples from goats and from dogs, respectively. The sequence of the amplicons exhibited 99% sequence similarity with the homologous sequence of the htpAB gene of C. burnetii RSA 331 (GenBank - CP000890). Conclusions The results confirm C. burnetii infection in animals in Rio de Janeiro and reinforce the need for the surveillance of Q fever in Brazil.

Este estudo teve como objetivos caracterizar florística e estruturalmente um trecho de Floresta Ombrófila Densa Submontana urbana e avaliar os fatores que contribuíram para a regeneração, a partir do último uso do solo para produção de banana, há 50 anos. Para a amostragem da área foram implantadas 25 parcelas de 100 m², totalizando 0,25 ha. O critério de inclusão adotado foi diâmetro à altura do peito (DAP) > 5 cm. Foram amostrados 311 indivíduos de 92 espécies, 67 gêneros e 31 famílias. A área basal total foi de 34,18 m²/ha, enquanto a densidade, de 1.244 ind./ha. As espécies mais importantes na comunidade, representando 42% do valor de importância (VI) da área, foram: Aiouea saligna Meisn., Tachigali paratyensis (Vell.) H.C. Lima, Ficus insipida Willd., Bathysa gymonocarpa K. Schum, Chrysophyllum flexuosum Mart., Piptadenia gonoacantha (Mart.) J.F. Macbr., Piper rivinoides Kunth., Hyeronima alchorneoides Allemão, Miconia cinnamomifolia (DC.) Naudin e Guarea guidonia (L.) Sleumer. O elevado valor do Índice de diversidade de Shannon (H'= 4,13 nats/ind.), bem como o de equabilidade (J = 0,91), compara-se aos valores referenciados para florestas conservadas e inventariadas no Sudeste brasileiro. A floresta amostrada encontra-se em processo de regeneração e representa um estágio intermediário de sucessão. O cultivo da banana, após seu abandono, permitiu a entrada de espécies com estratégias de estabelecimento e propagação em condições de pouca luminosidade. A presença de uma árvore remanescente, do gênero Ficus, está relacionada a uma crença popular que acabou influenciando a estrutura da vegetação. Dessa forma, as espécies amostradas neste estudo refletiram o uso do solo passado e a cultura local.

The objective of this study was to determine the structure and composition of an Atlantic Rain Forest tract with a land use history of banana plantation 50 year ago and evaluate the factors that influence its regeneration. Individual trees and shrubs with diameter at breast height (dbh) >5 cm were sampled within 25 plots of 10 x 10 m for a total sample size of 0.25 ha. We found 311 stems distributed across 92 species, 67 genera and 31 families, with a basal area of 34.18 m²/ha and a density of 1,244 ind./ha. The ten species with the highest importance value(IV) were the following: Aiouea saligna Meisn., Tachigali paratyensis(Vell.)H.C. Lima, Ficus insipida Willd., Bathysa gymonocarpa K. Schum, Chrysophyllum flexuosum Mart., Piptadenia gonoacantha (Mart.) J.F. Macbr., Piper rivinoides Kunth., Hyeronima alchorneoides Allemão, Miconia cinnamomifolia (DC.) Naudin and Guarea guidonia (L.) Sleumer. The high Shannon richness index and Pielou index (H'= 4.13 nats/ind. and J'= 0.91), are close to those of preserved forest stands of AtlanticRain Forest, suggesting a good recovery process for this stand, representing an intermediary stage of successional dynamics. The abandonment of the banana plantation created an environment of heterogeneous light conditions, allowing for the entry of species with different establishment strategies. The presence of a remnant tree of the Ficus genus, is related to a popular belief that ended up influencing the vegetation structure. Thus, the species sampled in this study reflected the past land use and the local culture.

Inúmeros trabalhos descrevem tanto o aspecto fisionômico e florístico do cerradão, no entanto, não há padronização do critério de inclusão, esforço amostral e tamanho das unidades amostrais o que dificulta as comparações e emergência de perfil estrutural para a vegetação lenhosa desta fitofisionomia. Buscamos com este trabalho descrever o perfil florístico-estrutural do componente lenhoso do cerradão, bem como avaliar o grau de sobreposição de espécies florestais, savânicas e generalistas. O trabalho foi realizado em seis áreas distribuídas nos estados de: MG, MS, MT, TO, PI e DF. A vegetação lenhosa do cerradão não apresentou estrutura homogênea ou consistência florística, apresentando-se como fisionomia bastante heterogênea. Estruturalmente a vegetação lenhosa apresentou grandes variações, com formações mais abertas e mais densas (densidade variando de 652 a 1.732 ind. ha-1 e área basal variando de 12,83 a 22,45 m² ha-1). A riqueza de espécies nas áreas vaiou de 49 (MS) a 124 (MT). Das 308 espécies registradas nas seis áreas apenas seis ocorreram em todas elas e 184 espécies foram exclusivas de uma das áreas. Florísticamente houve grande variação na composição de espécies e marcada dissimilaridade entre as áreas. Apesar de haver uma mistura de elementos florísticos savânicos e florestais, detectamos maior domínio de espécies savânicas com exceção da área MT que apresentou maior proporção de espécies florestais. O padrão geral de dissimilaridade florística e estrutural nas seis áreas indicaram grande heterogeneidade da distribuição da flora lenhosa do cerradão, seguindo o padrão de outras fitofisionomias do bioma Cerrado.

A large number of studies describes the structure and composition of cerradão, however there is no standardization regarding data collection, rendering it difficult to conduct ample comparisons and the emergence of a structural and floristic profile for the cerradão. Our aim is to establish a structural-floristic profile of the cerradão woody component and verify the overlap of forest, savanna and generalist species. We conducted the vegetation survey in sites in six states: DF, MG, MS, MT, PI and TO. The cerradão did not present a homogenous structure or floristic consistency among sites, presenting itself as a heterogeneous phytophysiognomy. Structurally it presented great variations, with both open and closed formations (density 652-1732 ind. ha-1; basal area 12.83-22.45 m² ha-1). Species richness varied from 49 (MS) to 129 (MT). Floristical variation and dissimilarity was strong among the six sites with only six species occurring in all sites, where as 184 species are exclusive to one site. Although species composition is comprised of a mixture of forest and savanna species, we detected a greater participation of savanna species, with the exception of the MT site that presented a larger proportion of forest species. The general floristic and structural dissimilarity pattern observed indicate a great heterogeneity of cerradão woody flora distribution following the general patter for the Cerrado biome.

We characterised hantaviruses circulating in different Akodon rodent species collected in midwestern Santa Catarina (SC), southern Brazil, where the Jabora hantavirus (JABV) strain was first identified in Akodon montensis. Genetic and phylogenetic analyses based on a partial S segment indicated that, in SC, Akodon paranaensis and A. montensis carried the same type of hantavirus. Additionally, we conducted the first genomic characterisation of the complete S segment from the Brazilian JABV strain. This is the first report of A. paranaensis infected with the JABV.

Dois experimentos foram conduzidos para avaliar o efeito da injeção intramuscular de betacaroteno associada ao tocoferol, na concentração plasmática de progesterona de novilhas Holandesas superovuladas (Experimento 1) e em novilhas cruzadas (Bos taurus x Bos indicus) submetidas à transferência embrionária em tempo fixo (TETF, experimento 2). No experimento 1, após a sincronização do estro e superovulação, os animais foram inseminados entre 12 a 24 h após o início do estro e os embriões recuperados após 7 dias. As novilhas foram divididas, aleatoriamente, em 1 de 3 tratamentos: Controle (duas injeções de soro); T800 (800 mg de betacaroteno mais 500 mg de tocoferol) e T1200 (1.200 mg de betacaroteno mais 750 mg de tocoferol). Os tratamentos foram administrados no dia da colocação do implante de progesterona e repetido no primeiro dia da superovulação. As colheitas de sangue foram realizadas no D0, D5, D9, D12 e D16. No experimento 2, os tratamentos foram realizados no momento da inserção do dispositivo de progesterona (D0). Utilizou-se o mesmo delineamento do experimento 1. A colheita de sangue para a determinação da progesterona por radioimunoensaio foi realizada no D17 (inovulação embrionária). No experimento 1, a concentração plasmática média, após formação do corpo lúteo (média da soma de D12 com D16) foi de 13,7±1,8 ng/ml, 14,5±2,3 ng/ml e 10,8±2,3 ng/ml para os grupos Controle, T800 e T1200, respectivamente, e não diferiram (P=0,44) entre os tratamentos. No experimento 2, a concentração de progesterona nas receptoras, no dia 17, foi semelhante (P=0,11) entre os grupos controle (8,88±0,57 ng/ml), T800 (7,48±0,64 ng/ml) e T1200 (5,90±1,33 ng/ml). Os resultados indicaram que as injeções de suplemento com betacaroteno e tocoferol não influenciaram na concentração plasmática de progesterona em novilhas Holandesas doadoras de embriões e em novilhas cruzadas, receptoras de embrião.

Two experiments were conducted to evaluate the effect of the intramuscular injection of betacarotene associated to tocopherol on the plasma concentration progesterone of superovulated Holstein heifers (experiment 1) and in crossbred (Bos taurus x Bos indicus) heifers submitted to fixed-time embryo transfer (FTET, experiment 2). In experiment 1, after estrus synchronization and superovulation animals were inseminated 12 and 24 hours after estrus onset and embryos flushed 7 days later. Heifers were allocated randomly to one of three treatments: Control; T800 (800 mg of betacarotene plus 500 mg of tocopherol) and T1200 (1,200 mg of betacarotene plus 750 mg of tocopherol). The treatments were given on the day of ear implant placement and repeated on the first day of superovulation. Blood samples were collected on D0, D5, D9, D12 and D16. In experiment 2, treatments were imposed at intravaginal device insertion (D0). The same experimental design, as in experiment 1, was used. Blood samples were collected on D17 (embryos implanted) for progesterone determination by radioimmunoassay. In experiment 1, average plasma progesterone concentrations after corpora lutea formation (D12 plus D16 means) were 13.7±1.8 ng/ml, 14.5±2.3 ng/ml and 10.8±2.3 ng/ml for control, T800 and T1200, respectively, and did not differ (P=0.44). In experiment 2, progesterone concentrations on D17 in Control (8.88±0.57 ng/ml), T800 (7.48±0.64 ng/ml) and T1200 (5.90±1.33 ng/ml) groups were similar (P=0.11). Results indicate that the supplemental betacarotene and tocopherol injections did not influence peripheral progesterone concentrations in superovulated Holstein donors and crossbreed recipients heifers.