ABSTRACT From October 1994 to September 1996, in the municipality of Mangaratiba, Rio de Janeiro State, phlebotomines were captured on the walls of the houses by means of a manual suction tube and light traps, in the household, the peridomicile and in the forest. As it is an area undergoing a real expansion and an increment in the local tourism, with the purpose of assessing changes in the phlebotomine fauna caused by environmental changes in the region, new captures were made in the same location from October 2015 to September 2016, this time using only light traps. In the two phases of the study, a total of 6,681 phlebotomines were captured. The results indicated that Ny. intermedia and Mg. migonei are fully adapted to this anthropic environment, while Pi. fischeri was more abundant and eclectic, and was probably attracted to exercise hematophagy. Nyssomyia intermedia can be suggested as the main vector of the etiological agent of the American Tegumentary Leishmaniasis in the studied areas. Pintomyia fischeri can also transmit Leishmania braziliensis, both in the environment altered by human action, and in the wild environment. Phlebotomines were captured in greater numbers between 6 and 8 pm in the peridomicile and between 9 and 11 pm in the household. The highest densities of phlebotomines were recorded in December, January and February. Despite almost 20 years between the two studies in the municipality of Mangaratiba, there was no change in the profile of the phlebotomine fauna in general; however, there was a greater density of species of medical importance in areas that suffered environmental impacts.

Abstract Associated with the use of removable prostheses, the development of candidiasis, called prosthetic stomatitis, is frequently observed. In view of the application of silver nanoparticles (AgNP) in dentistry that may offer antimicrobial effect, the aim of this study was to evaluate the effect of adding AgNP with different concentrations during thermopolymerization or immersion of acrylic in this substance in the properties antifungal, mechanical and cytotoxic. The groups were divided: addition of 1% silver nanoparticle solution (G1), addition of 2.5% silver nanoparticle solution (G2), addition of 5% silver nanoparticle solution (G3), immersed for 10 min in aqueous silver nanoparticle solution (G4), immersed for 24 hours in aqueous silver nanoparticle solution (G5). In the cytotoxicity assay, at all evaluation times, all groups showed cytotoxic effect (p <0.05) when compared to the control group (CG). For the microbiological assay, C. albicans reduction was observed only for G4 and G5 when compared to CG (p <0.05). The lowest resistance values were observed in the group with 5% silver nanoparticle (G3) incorporation (p <0.05). It was concluded that the thermopolymerized acrylic resin immersed in AgNP, G4 and G5 promoted microbiological reduction, cytotoxicity increase and flexural strength decrease at 5% concentration.

Abstract INTRODUCTION: American cutaneous leishmaniasis (ACL) is regarded as a public health problem in the Oiapoque basin, between Brazil and French Guiana. METHODS Data on ACL occurrence/epidemiological profile and etiology were sourced from Brazilian health services and a reference laboratory. Rainfall correlation was also analyzed. RESULTS: Majority of the ACL cases were observed in adult men working as gold miners. ACL incidence peaks appeared to be linked to periods 2 months after the dry season. Migratory flow was found to be a non-negligible complicating factor in epidemiological surveillance. CONCLUSIONS: Binational strategies are required to minimize exposure for high-risk populations.

BACKGROUND There is little information on the effect of using deltamethrin-impregnated dog collars for the control of canine visceral leishmaniasis. OBJECTIVES The objective of this study was to evaluate the effectiveness of the use of 4% deltamethrin-impregnated collars (Scalibor®) in controlling visceral leishmaniasis in Lutzomyia longipalpis by comparing populations in intervention and non-intervention areas. METHODS Phlebotomine flies were captured over 30 months in four neighbourhoods with intense visceral leishmaniasis transmission in Fortaleza and Montes Claros. We calculated the rates of domicile infestation, relative abundance of Lu. longipalpis, and Lu. longipalpis distribution in each site, capture location (intra- and peridomestic locations) and area (intervention and non-intervention areas). FINDINGS In the control area in Fortaleza, the relative abundance of Lu. longipalpis was 415 specimens at each capture site, whereas in the intervention area it was 159.25; in Montes Claros, the relative abundance was 5,660 specimens per capture site in the control area, whereas in the intervention area it was 2,499.4. The use of dog collars was associated with a reduction in captured insects of 15% (p = 0.004) and 60% (p < 0.001) in Montes Claros and Fortaleza, respectively. MAIN CONCLUSIONS We observed a lower vector abundance in the intervention areas, suggesting an effect of the insecticide-impregnated collars.

Abstract: INTRODUCTION: The present note discusses some evidence on the increasing potential risk for American visceral leishmaniasis (AVL) transmission in the Northern Brazilian State of Amapá, the Guianan-Amazon biome. METHODS Early and present data about AVL were collected, including our recent entomological findings. RESULTS: The spread of the sand fly vector Lutzomyia longipalpis, and a sylvatic reservoir host, the crab-eating fox Cerdocyon thous in that region represents important findings related to the epidemiology of AVL in the Guianan-Amazon biome. CONCLUSIONS: These observations suggest that Brazilian authorities need to develop surveillance strategies in these risk areas.

Since the description of Lutzomyia longipalpis by Lutz and Neiva more than 100 years ago, much has been written in the scientific literature about this phlebotomine species. Soares and Turco (2003) and Lainson and Rangel (2005) have written extensive reviews focused on vector-host-parasite interactions and American visceral leishmaniasis ecology. However, during the last two decades, the success of Lu. longipalpis in colonising urban environments and its simultaneous geographical spreading have led to new theoretical and operational questions. Therefore, this review updates the general information about this species and notes the more challenging topics regarding the new scenario of urbanisation-spreading and its control in America. Here, we summarise the literature on these issues and the remaining unsolved questions, which pose recommendations for operational research.

INTRODUCTION: The presence of American cutaneous leishmaniasis (ACL) in the communities of the Campus FIOCRUZ Mata Atlântica (CFMA) in the City of Rio de Janeiro initiated the investigation of the Phlebotominae fauna in the Atlantic Forest to determine the occurrence of putative ACL vectors associated with the enzootic cycle. METHODS: For 24 consecutive months, sand flies were captured inside the forest and in the border area near the communities. RESULTS: The following sand fly species were identified: Brumptomyia brumpti, Brumptomyia cunhai, Brumptomyia nitzulescui, Lutzomyia edwardsi, Lutzomyia pelloni, and Lutzomyia quinquefer. Other identified sand fly vectors, such as Lutzomyia intermedia (the predominant species), Lutzomyia migonei, Lutzomyia whitmani, Lutzomyia fischeri, and Lutzomyia hirsuta hirsuta, are associated with ACL transmission, and the vector for American visceral leishmaniases (AVL), Lutzomyia longipalpis, was also found. CONCLUSIONS: All sand fly vectors were found in both studied environments except for Lutzomyia whitmani, which was only identified in the forest. This study represents the first identification of Lutzomyia longipalpis in the CFMA, and the epidemiological implications are discussed.

O principal vetor de leishmaniose visceral (LV) no Brasil é Lutzomyia longipalpis. Entretanto, a ausência de L. longipalpis em área com casos autóctones de LV demonstra a existência de outras espécies na transmissão dessa doença. Estudo realizado na cidade de La Banda, Argentina, e São Vicente Férrer, Brasil, correlacionou a ausência de L. longipalpis e a presença de Lutzomyia migoneicom casos autóctones de LV. Em São Vicente Férrer, foi comprovada a infecção natural de L. migonei por Leishmania infantum chagasi. Dessa forma, o objetivo deste trabalho foi avaliar a ecologia dos flebotomíneos L. longipalpis e L. migonei no município de Fortaleza, área endêmica para LV. A captura de flebotomíneos foi realizada em 22 pontos de coleta distribuídos nas quatro regiões do município de Fortaleza. No total, foram capturados 32.403 flebotomíneos. Destes, 18.166 (56%) eram da espécie L. longipalpis e 14.237 (44%) eram L. migonei. Houve diferença significativa de densidade entre os vetores em cada local de captura (intra e peri) (p<0,0001). Esses achados confirmam que, na cidade de Fortaleza, L. migonei e L. longipalpis estão bem distribuídos, bem como adaptados ao ambiente intradomiciliar e que L. migonei, possivelmente, compartilhe com L. longipalpis o papel de vetor da LV em Fortaleza.

The main vector for visceral leishmaniasis (VL) in Brazil is Lutzomyia longipalpis. However, the absence of L. longipalpis in a region of autochthonous VL demonstrates the participation of other species in the transmission of the parasite. Studies conducted in La Banda, Argentina, and São Vicente Férrer, Pernambuco State, Brazil, have correlated the absence of L. longipalpisand the presence of L. migonei with autochthonous cases of VL. In São Vicente Férrer, Pernambuco, there was evidence for the natural infection of L. migonei with Leishmania infantum chagasi. Thus, the objective of this work was to assess the ecology of the sand flies L. longipalpis and L. migonei in Fortaleza, an endemic area for VL. Insect capture was conducted at 22 sampling points distributed across four regions of Fortaleza. In total, 32,403 sand flies were captured; of these, 18,166 (56%) were identified as L. longipalpis and 14,237 (44%) as L. migonei. There were significant density differences found between the vectors at each sampling site (indoors and outdoors) (p <0.0001). These findings confirm that L. migonei and L. longipalpis are distributed throughout Fortaleza, where they have adapted to an indoor environment, and suggest that L. migonei may share the role as a vector with L. longipalpis in the transmission of VL in Fortaleza.

Phlebotomine sand flies (Diptera, Psychodidae) from Rio de Janeiro State, Brazil: Species distribution and potential vectors of leishmaniases. Rio de Janeiro State, in Brazil, has endemic areas of both cutaneous and visceral leishmaniases. In these areas, entomologic surveillance actions are highly recommended by Brazil's Ministry of Health. The present work describes the results of sand fly captures performed by the Health Department of Rio de Janeiro State between 2009 and 2011 in several municipalities. An updated species list and distribution of phlebotomine sand flies in the state are provided based on an extensive literature review. Currently, the sand fly fauna of Rio de Janeiro State has 65 species, belonging to the genera Brumptomyia (8 spp.) and Lutzomyia (57 spp.). Distribution maps of potential leishmaniases vector species Lutzomyia (Nyssomyia) intermedia, L. migonei, L. (N.) whitmani, L. (N.) flaviscutellata and L. (Lutzomyia) longipalpis are provided and their epidemiological importance is discussed.

Phlebotomine sandflies were captured in rural settlement and periurban areas of the municipality of Guaraí in the state of Tocantins (TO), an endemic area of American cutaneous leishmaniasis (ACL). Forty-three phlebotomine species were identified, nine of which have already been recognised as ACL vectors. Eleven species were recorded for the first time in TO. Nyssomyia whitmani was the most abundant species, followed by Evandromyia bourrouli, Nyssomyia antunesi and Psychodopygus complexus. The Shannon-Wiener diversity index and the evenness index were higher in the rural settlement area than in the periurban area. The evaluation of different ecotopes within the rural area showed the highest frequencies of Ev. bourrouli and Ny. antunesi in chicken coops, whereas Ny. whitmani predominated in this ecotope in the periurban area. In the rural settlement area, Ev. bourrouli was the most frequently captured species in automatic light traps and Ps. complexus was the most prevalent in Shannon trap captures. The rural settlement environment exhibited greater phlebotomine biodiversity than the periurban area. Ps. complexus and Psychodopygus ayrozai naturally infected with Leishmania (Viannia) braziliensis were identified. The data identified Ny. whitmani as a potential ACL vector in the periurban area, whereas Ps. complexus was more prevalent in the rural environment associated with settlements.

The aim of the present review is to give relevant information on aspects of the biology and ecology, including the vectorial competence of Lutzomyia sand fly species suggested as vectors of American cutaneous leishmaniasis in Brazil. The disease, due to Leishmania (Viannia) braziliensis, has been registered in most municipalities in all the Brazilian states and its transmission is associated with more than one sand fly species in each geographical region. A variety of Leishmania species can be found in the Amazon basin, where different epidemiological chains have been detected with the participation of different phlebotomine vectors. Finally, a discussion is presented on some sand fly species found naturally infected by Leishmania, but for which there is as yet no evidence regarding their epidemiological importance.

O teste de precipitina foi aplicado na identificação das fontes alimentares de Lutzomyia intermedia, de ambientes intra e peri-domiciliar, do Município de Mesquita, Estado do Rio de Janeiro, Brasil, área de transmissão de Leishmania (V.) braziliensis. Foram testados os seguintes antisoros: homem, ave, cão, eqüino, gambá e roedor. De um total de 370 fêmeas analisadas, revelaram reatividade, contra os antisoros testados, 128 espécimes do intra-domicílio e 59 do ambiente peridomiciliar. A antropofilia de L. intermedia foi confirmada nos dois ambientes trabalhados; da mesma forma, a alimentação deste flebotomíneo em animais domésticos, observada anteriormente em levantamentos entomológicos, foi comprovada pela forte reatividade com antisoros de ave, cão e eqüino. Entretanto, a alimentação em roedores, animais sinantrópicos no ambiente domiciliar (interior e ao redor das residências), é mais uma forte evidência da competência vetorial de L. intermedia, uma vez que roedores sinantrópicos e silvestres são comprovadamente reservatórios de L. (V.) braziliensis.

The precipitin test was applied to identify the blood meal sources of Lutzomyia intermedia, collected in two distinct habitats: inside houses and in the peridomicile, in Mesquita, Rio de Janeiro State, Brazil, a transmission area of Leishmania (V.) braziliensis. The following antisera were tested: human, avian, equine, rodent, and opossum. From a total of 370 females analyzed, 128 specimens from the domicile and 59 from the peridomicile reacted with specific antisera. The anthropophily of L. intermedia was confirmed in both habitats; likewise, the feeding of this sand fly species on domestic animals, observed in previous entomological surveys, was confirmed by the strong reactivity with avian, canine, and equine antisera. However, feeding on rodents, mammals frequently found inside and around houses, represents further evidence related to the vector competence of L. intermedia, since synanthropic and sylvatic rodents have been considered a putative reservoir of L. (V.) braziliensis.

O teste de precipitina foi aplicado na identificação das fontes alimentares de Lutzomyia intermedia, de ambientes intra e peri-domiciliar, do Município de Mesquita, Estado do Rio de Janeiro, Brasil, área de transmissão de Leishmania (V.) braziliensis. Foram testados os seguintes antisoros: homem, ave, cão, eqüino, gambá e roedor. De um total de 370 fêmeas analisadas, revelaram reatividade, contra os antisoros testados, 128 espécimes do intra-domicílio e 59 do ambiente peridomiciliar. A antropofilia de L. intermedia foi confirmada nos dois ambientes trabalhados; da mesma forma, a alimentação deste flebotomíneo em animais domésticos, observada anteriormente em levantamentos entomológicos, foi comprovada pela forte reatividade com antisoros de ave, cão e eqüino. Entretanto, a alimentação em roedores, animais sinantrópicos no ambiente domiciliar (interior e ao redor das residências), é mais uma forte evidência da competência vetorial de L. intermedia, uma vez que roedores sinantrópicos e silvestres são comprovadamente reservatórios de L. (V.) braziliensis.

The precipitin test was applied to identify the blood meal sources of Lutzomyia intermedia, collected in two distinct habitats: inside houses and in the peridomicile, in Mesquita, Rio de Janeiro State, Brazil, a transmission area of Leishmania (V.) braziliensis. The following antisera were tested: human, avian, equine, rodent, and opossum. From a total of 370 females analyzed, 128 specimens from the domicile and 59 from the peridomicile reacted with specific antisera. The anthropophily of L. intermedia was confirmed in both habitats; likewise, the feeding of this sand fly species on domestic animals, observed in previous entomological surveys, was confirmed by the strong reactivity with avian, canine, and equine antisera. However, feeding on rodents, mammals frequently found inside and around houses, represents further evidence related to the vector competence of L. intermedia, since synanthropic and sylvatic rodents have been considered a putative reservoir of L. (V.) braziliensis.

The development of Colombian Leishmania species of the subgenus Viannia in Lutzomyia intermedia was similar to that observed with Brazilian Le. (V.) braziliensis: colonization of the pylorus by paramastigotes; promastigotes in the midgut and massive infection of stomodeal valve. Difference was observed in the number of paramastigotes colonizing the pylorus, which was smaller in Colombian Leishmania species than Brazilian Le. braziliensis.

O achado de uma mula infectada num foco endêmico de leishmaniose tegumentar no Rio de Janeiro, levou-nos a procurar sistematicamente infecções por Leishmania em equinos, resultando no encontro de 30,8% de parasitados, incluindo cavalos e mulas. A possibilidade de esses animais participarem da cadeia epidemiológica da leishmaniose humana está sendo investigada.

In an endemic area of cutaneous leishmaniasis in Rio de Janeiro State where a mule had been found infected, a systematic search among equines was performed, resulting in the detection of Leishmania parasites in skin lesions of 30.8% of the animals, which included horses and mules. The eventual role of equines in the epidemiology of the human disease is being investigated.