ABSTRACT Blockchain technology, initially applied to the financial market, is recognized for its security in storing information in a decentralized manner and its ease of access to this information. An application of blockchain technology is the establishment of traceability systems for production chains. Food traceability systems are becoming increasingly important in guaranteeing the quality and safety of food to the final consumer, who, in turn, values and exercises confidence in the traceability system, recognizing its importance. This study was conducted to provide a systematic literature review on the importance of applying blockchain technology to the traceability of products of animal origin. Two scientific databases were consulted: Scopus and Web of Science. After classification based on the inclusion and exclusion criteria, 12 articles were selected for the study. The application of blockchain technology is recent, emerging, and promising in the traceability of animal products, and it has attracted research interest worldwide. The use of blockchain technology in the traceability of products of animal origin is used in several production chains, providing consumers with long-awaited information about the origin of their food such as beef, milk, fish, meat, pork, and eggs. market chains consumer who turn system consulted Science criteria 1 recent emerging worldwide longawaited long awaited beef milk fish meat pork eggs

Abstract Introduction Perianal fistula is a common colorectal disease which is caused mainly by cryptoglandular disease. Although most cases are treated successfully by surgery, management of complex perianal fistulas (CPAF) remains a challenge with limited results in recurrence and sometimes associated with fecal incontinence. The CPAF treatment with autologous adipose-derived mesenchymal stem cells (ASCs) had become a research hotspot. The technique started to be used in the treatment of Crohn's disease (CD) fistulas, where the studies showed safe and goods result from the procedure. Cultured ASCs have been used but this approach requires the preceding collection of adipose tissue, time for isolation of ASCs and subsequent in vitro expansion, need for laboratory facilities, and expertise in cell culturing. These factors have been getting over by using the commercially available alternative, allogenic ASCs. Treatment with allogeneic ASCs has shown good results in patients with CD fistulas, however with the disadvantage of being expensive. Objective To show that the injection with freshly collected adipose tissue is an alternative to treatment with autologous or allogenic ASCs with several advantages. Methods: In this case report, we show our first experience in the treatment of CPAF with the application of collected adipose tissue in a tertiary referral hospital from Belo Horizonte, Brazil. Results The patient had a good postoperative recuperation with a complete fistula healing after 8 months without adverse effects. Conclusion Injection with freshly collected adipose tissue is a promising and apparently safe sphincter-sparing technique in the treatment of CPAF. surgery (CPAF incontinence adiposederived derived (ASCs hotspot Crohns Crohn s (CD procedure expansion facilities culturing expensive advantages Methods report Horizonte Brazil effects sphinctersparing sphincter sparing

ABSTRACT Background: SARS-CoV-2 virus originated in Wuhan (China) in December (2019) and quickly spread worldwide. Antigen tests are rapid diagnostic tests (RDT) that produce results in 15-30 min and are an important tool for the scale-up of COVID-19 testing. COVID-19 diagnostic tests are authorized for self-testing at home in some countries, including Brazil. Widespread COVID-19 diagnostic testing is required to guide public health policies and control the speed of transmission and economic recovery. Methods: Patients with suspected COVID-19 were recruited at the Hospital da Baleia (Belo Horizonte, Brazil). The SARS-CoV-2 antigen-detecting rapid diagnostic tests were evaluated from June 2020 to June 2021 using saliva, nasal, and nasopharyngeal swab samples from 609 patients. Patient samples were simultaneously tested using a molecular assay (RT-qPCR). Sensitivity, specificity, accuracy, and positive and negative predictive values were determined using the statistical program, MedCalc, and GraphPad Prism 8.0. Results: The antigen-detecting rapid diagnostic tests displayed 98% specificity, 60% sensitivity, 96% positive predictive value, and moderate concordance with RT-qPCR. Substantial agreement was found between the two methods for patients tested < 7 days of symptom onset. Conclusions: Our findings support the use of Ag-RDT as a valuable and safe diagnostic method. Ag-RDT was also demonstrated to be an important triage tool for suspected COVID-19 patients in emergencies. Overall, Ag-RDT is an effective strategy for reducing the spread of SARS-CoV-2 and contributing to COVID-19 control. Background SARSCoV2 SARSCoV SARS CoV 2 SARS-CoV- China (China 2019 (2019 worldwide RDT (RDT 1530 15 30 15-3 scaleup scale up COVID19 COVID 19 COVID-1 selftesting self countries Brazil recovery Methods Belo Horizonte . Brazil) antigendetecting antigen detecting 202 saliva nasal 60 RTqPCR. RTqPCR RT qPCR (RT-qPCR) Sensitivity specificity accuracy program MedCalc 80 8 0 8.0 Results 98 sensitivity 96 value qPCR. RT-qPCR onset Conclusions AgRDT Ag method emergencies Overall SARS-CoV 201 (201 153 1 3 15- COVID1 COVID- 20 6 (RT-qPCR 8. 9 (20 (2 (

RESUMO Introdução: a colonoscopia é o melhor método para detecção de pólipos, com redução da mortalidade por câncer colorretal de 29% e chegando até 47% para tumores distais. No entanto, existe falha em demonstrar redução significativa na mortalidade no cólon proximal sendo o segmento mais comum de neoplasia de intervalo. O presente estudo avaliou o impacto na detecção de pólipos em uma segunda avaliação sequencial do ceco e cólon ascendente, com ou sem o uso de cromoendoscopia com Indigo carmim. Métodos: estudo prospectivo, não randomizado. Os pacientes foram divididos em dois grupos. O primeiro (G1) foi submetido à colonoscopia de rotina, seguida de segunda avaliação endoscópica do cólon ascendente e ceco. O segundo grupo (G2) foi submetido à colonoscopia de rotina, seguida de segunda avaliação do cólon ascendente e ceco com cromoendoscopia com índigo carmim. Resultados: no total, foram analisados 203 pacientes, sendo 101 do G1 e 102 do G2. Novos pólipos foram identificados em ambos os grupos após a segunda avaliação com número significativamente maior de pólipos detectados nos pacientes do G2 (p=0,0001). O número de pacientes que apresentaram pelo menos um pólipo nas duas avaliações endoscópicas foi significativamente maior no G2 (53 ou 52% vs 27 ou 26,7%, p=0,0002). Na segunda avaliação endoscópica, o número de pólipos encontrados também foi significativamente maior no G2 (50 ou 76,9%) em relação ao G1 (15 ou 23,1%), p<0,000. Conclusão: a segunda avaliação com cromoendoscopia com índigo carmim aumenta a detecção de pólipos no cólon ascendente e no ceco. Introdução 29 47 distais entanto intervalo Métodos prospectivo randomizado G (G1 rotina (G2 Resultados total 20 10 p=0,0001. p00001 p p=0,0001 . 0 0001 (p=0,0001) 53 (5 52 2 267 26 7 26,7% p=0,0002. p00002 p=0,0002 0002 p=0,0002) 50 76,9% 769 76 9 15 (1 23,1%, 231 23,1% , 23 1 23,1%) p0000 000 p<0,000 Conclusão 4 (G p=0,000 (p=0,0001 5 ( 26,7 76,9 23,1 p000 00 p<0,00 p=0,00 (p=0,000 26, 76, 23, p00 p<0,0 p=0,0 (p=0,00 p0 p<0, p=0, (p=0,0 p<0 p=0 (p=0, p< p= (p=0 (p= (p

ABSTRACT Introduction: colonoscopy is the best method for detecting polyps, with a reduction in colorectal cancer mortality of 29% and reaching 47% for distal tumors. However, it fails to demonstrate a significant reduction in proximal colon cancer mortality, and is the most common segment with interval neoplasm. The present study aimed to evaluate the impact on detection of polyps of a second sequential evaluation of cecum and ascending colon, with or without the use of indigo carmine chromoendoscopy. Methods: prospective, non-randomized clinical trial. Patients were divided into two groups. The first (G1) underwent a routine colonoscopy, followed by a second endoscopy assessment of ascending colon and cecum. The second group (G2) underwent a routine colonoscopy, followed by a second assessment of the ascending colon and cecum with indigo carmine chromoendoscopy. Results: In total, 203 patients were analyzed, 101 in the G1 and 102 in the G2. Newer polyps were identified in both groups after the second assessment with a significantly higher number of polyps detected in the patients in the G2 (p=0.0001). The number of patients who had at least one polyp in the two endoscopic assessments was significantly higher in the G2 (53 or 52% vs 27 or 26.7%, p=0.0002). In the second endoscopic assessment, the number of polyps found was also significantly higher in the G2 (50 or 76.9%) compared to the G1 (15 or 23.1%), p<0.0001. Conclusions: the second assessment with dye-based chromoendoscopy increases the detection of polyps in the ascending colon and cecum. Introduction 29 47 tumors However neoplasm Methods prospective nonrandomized non randomized trial G (G1 (G2 Results total 20 analyzed 10 p=0.0001. p00001 p p=0.0001 . 0 0001 (p=0.0001) 53 (5 52 2 267 26 7 26.7% p=0.0002. p00002 p=0.0002 0002 p=0.0002) 50 76.9% 769 76 9 15 (1 23.1%, 231 23.1% , 23 1 23.1%) p<0.0001 Conclusions dyebased dye based 4 (G p0000 p=0.000 000 (p=0.0001 5 ( 26.7 76.9 23.1 p<0.000 p000 p=0.00 00 (p=0.000 26. 76. 23. p<0.00 p00 p=0.0 (p=0.00 p<0.0 p0 p=0. (p=0.0 p<0. p=0 (p=0. p<0 p= (p=0 p< (p= (p

RESUMO Objetivo. Sintetizar os achados sobre correlatos da atividade física (AF) e do comportamento sedentário (CS) em crianças sul-americanas. Método. Realizou-se uma busca dos artigos no período de 24 de junho até 27 de outubro de 2020, nas bases de dados LILACS, PubMed, SciELO, Scopus e Web of Science. Para ampliar a busca, foram examinadas as referências dos artigos de revisão identificados e realizada uma consulta a um painel de especialistas. Foram incluídos estudos com delineamentos observacional e de intervenção com foco em crianças sul-americanas de zero a 5 anos de idade. Resultados. Dos 3 111 artigos inicialmente identificados, 18 foram elegíveis: 14 com delineamento observacional, 12 realizados no Brasil e 17 conduzidos com pré-escolares (3 a 5 anos de idade). Além do Brasil, os únicos países representados foram o Chile e o Equador. A AF e o CS foram medidos por acelerômetros em sete estudos, porém houve alta variabilidade nos instrumentos e pontos de corte usados. Embora as crianças tenham sido consideradas fisicamente ativas em 12 estudos, seis estudos mostraram que elas despendiam muito tempo em CS. Os domínios de influência mais avaliados foram o individual (14 estudos) e o interpessoal (11 estudos), seguidos pelo ambiental (oito estudos) e o político (um estudo). As intervenções no contexto escolar (quatro estudos) aumentaram os níveis de AF e diminuíram o tempo em CS. Entretanto, as evidências dos estudos transversais e de intervenção apresentaram alto risco de viés. Conclusão. Diante das lacunas identificadas, recomendam-se estudos com delineamentos robustos que incluam mais países sul-americanos, com foco em bebês e crianças com menos de 3 anos de idade e que investiguem correlatos dos domínios ambiental e político.

ABSTRACT Objective. To synthesize the findings on correlates of physical activity (PA) and sedentary behavior (SB) in South American children. Method. A search was carried out from June 24 to October 27, 2020, in the LILACS, PubMed, SciELO, Scopus and Web of Science databases. To broaden the search, the references of identified review articles were examined, and a panel of experts was surveyed. Observational and interventional studies conducted with South-American children from zero to 5 years of age were included. Results. Of 3 111 articles initially identified, 18 were eligible: 14 observational studies, 12 studies conducted in Brazil, and 17 studies investigating preschool children (3 to 5 years of age). In addition to Brazil, only Chile and Ecuador were represented. PA and SB were measured by accelerometers in seven studies; however, high variability was observed in the instruments and cut-off points used. Although children were classified as physically active in 12 studies, six studies showed that they spent considerable time in SB. Most studies assessed the individual (14 studies) and interpersonal (11 studies) domains, followed by the environmental (eight studies) and political (one study) domains. Interventions in the school context (four studies) increased PA levels and reduced the time spent in SB. However, the evidence from cross-sectional and interventional studies presented a high risk of bias. Conclusions. Given the knowledge gap identified in this review, studies with robust designs, covering additional South American countries are warranted, focusing on infants and children under 3 years of age and investigating correlates in the environmental and political domains.

RESUMEN Objetivo. Resumir los hallazgos sobre la correlación entre actividad física (AF) y comportamiento sedentario (CS) en la población infantil en América del Sur. Método. Se realizó una búsqueda de los artículos publicados entre el 24 de junio y el 27 de octubre del 2020 en las bases de datos LILACS, PubMed, SciELO, Scopus y Web of Science. Para ampliar la búsqueda, se examinaron las referencias de los artículos de revisión encontrados y se consultó con un panel de expertos. Se incluyeron estudios observacionales y de intervención centrados en población infantil de 0 a 5 años de edad en América del Sur. Resultados. De los 3 111 artículos encontrados inicialmente, 18 cumplieron con los criterios establecidos: 14 tenían un diseño observacional, 12 se llevaron a cabo en Brasil y 17 se realizaron con niños y niñas en edad preescolar (de 3 a 5 años). Además de Brasil, los únicos países representados fueron Chile y Ecuador. La AF y el CS se midieron con acelerómetros en 7 estudios, pero se observó una alta variabilidad en los instrumentos y los puntos de corte utilizados. Aunque se consideró que niños y niñas eran físicamente activos en 12 estudios, 6 estudios revelaron que pasaban mucho tiempo en CS. Los ámbitos de influencia más evaluados fueron el individual (14 estudios) y el interpersonal (11 estudios), seguidos del ambiental (8 estudios) y el político (1 estudio). Las intervenciones en el contexto escolar (4 estudios) aumentaron los niveles de AF y disminuyeron el tiempo en CS. Sin embargo, la evidencia de los estudios transversales y de intervención presentó un alto riesgo de sesgo. Conclusiones. En vista de las lagunas detectadas, se recomienda que se realicen estudios con diseño robusto que incluyan más países sudamericanos, se centren en bebés y menores de 3 años e investiguen correlaciones en los ámbitos político y ambiental.

RESUMO Blastopsylla occidentalis Taylor,1985 (Hemiptera: Aphalaridae) é um psilídeo que tem como hospedeiro o eucalipto, originário da Austrália, foi introduzido no Brasil em meados da década de 1990. Devido à escassez de informações a respeito da biologia desse inseto, este trabalho teve como objetivo conhecer o efeito da temperatura no desenvolvimento e reprodução do psilídeo sob condições de laboratório. O experimento foi realizado entre 2018 e 2019, em câmaras climatizadas reguladas em cinco temperaturas (15, 20, 25, 30 e 35°C). Os parâmetros observados nessa etapa do experimento foram: período de desenvolvimento dos ovos (número de dias, da postura até a eclosão); taxa de eclosão de imaturos; desenvolvimento de imaturos; taxa de emergência de adultos; longevidade, o período (em dia) de postura até a morte dos adultos da primeira geração (F1). Como resultado obteve-se o ciclo completo nas temperaturas 20, 25 e 30oC, com duração média de 32,78, 27,76 e 28,44 dias, respectivamente. Para a geração F1, não foram observadas postura na temperatura 30ºC, enquanto em 20 e 25ºC, a fecundidade média das fêmeas foi de 19,04 e 26,40, respectivamente. A duração média do período de incubação de ovos foi de 6,70 (20ºC) e 5,59 dias (25ºC). Portanto, infere-se que as temperaturas 15 e 35ºC são limitantes para o desenvolvimento do inseto, pois, durante o estudo, os imaturos de Blastopsylla occidentalis não completaram o ciclo de desenvolvimento. A faixa de temperatura ótima para o desenvolvimento e reprodução foi entre 20 e 25ºC, sendo que em 25ºC foi a temperatura em que o inseto apresentou menor mortalidade em todas as fases, menor tempo de desenvolvimento e maior número médio de ovos/dias. Espera-se que esses resultados sirvam para conduzir estudos para a aplicação de técnicas de manejo para eventual controle da espécie.

ABSTRACT Blastopsylla occidentalis Taylor, 1985 (Hemiptera: Aphalaridae) is a psyllid which host is the eucalyptus, originally from Australia, which was introduced in Brazil in the mid-1990s. Little is known on the biology of this insect. Here, we examine the effect of temperature on its development and the reproduction under laboratory conditions. The experiments were carried out in 2018 and 2019, in climate chambers regulated at five temperatures (15, 20, 25, 30 and 35°C). The parameters observed at this stage of the experiment were: period of the egg development (number of days, from laying to hatching); hatching rate of immatures; development of immatures; adult emergency rate; longevity, the period (in day) of laying until the death of the first generation adults (F1). Blastopsylla occidentalis completed the life cycle at temperatures of 20, 25 and 30C, with an average duration of 32.78, 27.76 and 28.44 days, respectively. In the F1 generation, no posture was observed at 30°C, while at 20 and 25°C, the average fertility was 19.04 and 26.40 eggs per female, respectively. The average duration of the egg incubation was 6.70 (20 °C) and 5.59 (25 °C) days. Immature B. occidentalis did not complete their development at 15 and 35°C and we conclude that these temperatures limit its development. The optimum temperature range for the development and the reproduction was between 20 and 25°C, with 25°C, being the temperature at which the insect had the lowest mortality in all stages, the shortest development time and the highest average number of eggs/days. It is hoped that these results will help to improve the management techniques for the control of the species.

O objetivo foi estimar a prevalência e analisar a associação do consumo de álcool e drogas ilícitas com indicadores de violência física em adolescentes. Estudo transversal repetido com estudantes do Ensino Médio em escolas da rede pública em Pernambuco, Brasil (2006, n = 4.207; 2011, n = 6.264). Os dados foram obtidos por questionário. Os indicadores de violência física foram: vitimização por violência física; envolvimento em brigas. As variáveis independentes foram: consumo de álcool e uso de drogas ilícitas. A regressão logística binária foi utilizada para verificar a associação entre o consumo de álcool e drogas e ter sofrido violência física. A prevalência do consumo de álcool por adolescentes que sofreram violência física reduziu em cinco anos, porém, nenhuma alteração foi constatada no uso de drogas ilícitas. As variáveis comportamentais estiveram associadas aos diferentes indicadores de violência, apresentando em geral uma tendência de aumento. Em 2006, adolescentes que usavam drogas ilícitas apresentaram uma chance 2,12 maior de sofrer violência física, e em 2011 este valor foi de 2,41 (IC95%: 1,80; 3,22). A chance de adolescentes que consumiam bebida alcoólica ter envolvimento em brigas aumentou de 1,96 (IC95%: 1,64; 2,34) em 2006, para 2,11 (IC95%: 1,78; 2,51) em 2011. O consumo de álcool pelos adolescentes que sofreram violência física diminuiu em cinco anos, entretanto, o risco de os adolescentes que consumiram bebida alcoólica ou usaram drogas ilícitas sofrerem violência física aumentou ao longo de cinco anos.

The article aimed to estimate prevalence and analyze the association between alcohol and illicit drug consumption and indicators of physical violence in adolescents. A repeated cross-sectional study was performed in public middle-school students in the State of Pernambuco, Brazil (2006, n = 4,207; 2011, n = 6,264). Data were obtained via a questionnaire. Indicators of physical violence were: victimization by physical violence and involvement in fights. The independent variables were alcohol and illicit drug consumption. Binary logistic regression was used to verify the association between alcohol and drug consumption and victimization from physical violence. Prevalence of alcohol consumption in adolescents that suffered physical violence decreased in five years, but no change appeared in the use of illicit drugs. The behavioral variables were associated with different indicators of violence, generally presenting an upward trend. In 2006, adolescents that used illicit drugs showed 2.12 higher odds of suffering physical violence, and in 2011 this value was 2.41 (95%CI: 1.80; 3.22). The odds of adolescents that consumed alcoholic beverages being involved in fights increased from 1.96 in 2006 (95%CI: 1.64; 2.34) to 2.11 in 2011 (95%CI: 1.78; 2.51). Alcohol consumption by adolescents that suffered physical violence decreased in five years, but the risk of adolescents that consumed alcohol or used illicit drugs and suffered physical violence increased over the course of five years.

El objetivo fue estimar la prevalencia y analizar la asociación del consumo de alcohol y drogas ilícitas con indicadores de violencia física en adolescentes. Se realizó un estudio transversal, repetido con estudiantes de enseñanza media en escuelas de la red pública en Pernambuco, Brasil (2006, n = 4.207; 2011, n = 6.264). Los datos se obtuvieron mediante cuestionario. Los indicadores de violencia física fueron: victimización por violencia física; implicación en peleas. Las variables independientes fueron consumo de alcohol y uso de drogas ilícitas. La regresión logística binaria fue utilizada para verificar la asociación entre consumo de alcohol y drogas y haber sufrido violencia física. La prevalencia del consumo de alcohol en adolescentes que sufrieron violencia física se redujo en cinco años, a pesar de que no se constató ninguna alteración en el uso de drogas ilícitas. Las variables comportamentales estuvieron asociadas a los diferentes indicadores de violencia, presentando en general una tendencia de aumento. En 2006, adolescentes que usaban drogas ilícitas presentaron una oportunidad 2,12 mayor de sufrir violencia física y en 2011 ese valor fue de 2,41 (IC95%: 1,80; 3,22). La oportunidad de adolescentes que consumían bebidas alcohólicas de que tuvieran implicación en peleas aumentó de 1,96 (IC95%: 1,64; 2,34) en 2006, a 2,11 (IC95% 1,78; 2,51) en 2011. El consumo de alcohol por parte de los adolescentes que sufrieron violencia física disminuyó en cinco años, no obstante, el riesgo de los adolescentes que consumieron bebidas alcohólica o consumieron drogas ilícitas sufrieran violencia física aumentó a lo largo de cinco años.

O objetivo foi estimar a prevalência e analisar a associação do consumo de álcool e drogas ilícitas com indicadores de violência física em adolescentes. Estudo transversal repetido com estudantes do Ensino Médio em escolas da rede pública em Pernambuco, Brasil (2006, n = 4.207; 2011, n = 6.264). Os dados foram obtidos por questionário. Os indicadores de violência física foram: vitimização por violência física; envolvimento em brigas. As variáveis independentes foram: consumo de álcool e uso de drogas ilícitas. A regressão logística binária foi utilizada para verificar a associação entre o consumo de álcool e drogas e ter sofrido violência física. A prevalência do consumo de álcool por adolescentes que sofreram violência física reduziu em cinco anos, porém, nenhuma alteração foi constatada no uso de drogas ilícitas. As variáveis comportamentais estiveram associadas aos diferentes indicadores de violência, apresentando em geral uma tendência de aumento. Em 2006, adolescentes que usavam drogas ilícitas apresentaram uma chance 2,12 maior de sofrer violência física, e em 2011 este valor foi de 2,41 (IC95%: 1,80; 3,22). A chance de adolescentes que consumiam bebida alcoólica ter envolvimento em brigas aumentou de 1,96 (IC95%: 1,64; 2,34) em 2006, para 2,11 (IC95%: 1,78; 2,51) em 2011. O consumo de álcool pelos adolescentes que sofreram violência física diminuiu em cinco anos, entretanto, o risco de os adolescentes que consumiram bebida alcoólica ou usaram drogas ilícitas sofrerem violência física aumentou ao longo de cinco anos.

The article aimed to estimate prevalence and analyze the association between alcohol and illicit drug consumption and indicators of physical violence in adolescents. A repeated cross-sectional study was performed in public middle-school students in the State of Pernambuco, Brazil (2006, n = 4,207; 2011, n = 6,264). Data were obtained via a questionnaire. Indicators of physical violence were: victimization by physical violence and involvement in fights. The independent variables were alcohol and illicit drug consumption. Binary logistic regression was used to verify the association between alcohol and drug consumption and victimization from physical violence. Prevalence of alcohol consumption in adolescents that suffered physical violence decreased in five years, but no change appeared in the use of illicit drugs. The behavioral variables were associated with different indicators of violence, generally presenting an upward trend. In 2006, adolescents that used illicit drugs showed 2.12 higher odds of suffering physical violence, and in 2011 this value was 2.41 (95%CI: 1.80; 3.22). The odds of adolescents that consumed alcoholic beverages being involved in fights increased from 1.96 in 2006 (95%CI: 1.64; 2.34) to 2.11 in 2011 (95%CI: 1.78; 2.51). Alcohol consumption by adolescents that suffered physical violence decreased in five years, but the risk of adolescents that consumed alcohol or used illicit drugs and suffered physical violence increased over the course of five years.

El objetivo fue estimar la prevalencia y analizar la asociación del consumo de alcohol y drogas ilícitas con indicadores de violencia física en adolescentes. Se realizó un estudio transversal, repetido con estudiantes de enseñanza media en escuelas de la red pública en Pernambuco, Brasil (2006, n = 4.207; 2011, n = 6.264). Los datos se obtuvieron mediante cuestionario. Los indicadores de violencia física fueron: victimización por violencia física; implicación en peleas. Las variables independientes fueron consumo de alcohol y uso de drogas ilícitas. La regresión logística binaria fue utilizada para verificar la asociación entre consumo de alcohol y drogas y haber sufrido violencia física. La prevalencia del consumo de alcohol en adolescentes que sufrieron violencia física se redujo en cinco años, a pesar de que no se constató ninguna alteración en el uso de drogas ilícitas. Las variables comportamentales estuvieron asociadas a los diferentes indicadores de violencia, presentando en general una tendencia de aumento. En 2006, adolescentes que usaban drogas ilícitas presentaron una oportunidad 2,12 mayor de sufrir violencia física y en 2011 ese valor fue de 2,41 (IC95%: 1,80; 3,22). La oportunidad de adolescentes que consumían bebidas alcohólicas de que tuvieran implicación en peleas aumentó de 1,96 (IC95%: 1,64; 2,34) en 2006, a 2,11 (IC95% 1,78; 2,51) en 2011. El consumo de alcohol por parte de los adolescentes que sufrieron violencia física disminuyó en cinco años, no obstante, el riesgo de los adolescentes que consumieron bebidas alcohólica o consumieron drogas ilícitas sufrieran violencia física aumentó a lo largo de cinco años.

Abstract INTRODUCTION: In Brazil, West Nile virus (WNV) was first detected, in 2018, in horses with neurological disease. AIM: We report the first case of WNV infection in a horse from Ceará state and the complete genome sequence of an isolate from Espírito Santo state. Both infections occurred in 2019. METHODS: WNV was isolated from the tissues of a horse with neurological signs in Espírito Santo and sequenced by MiSeq. RESULTS: Phylogenetic analysis revealed that the isolate belongs to lineage 1a, clustering with the NY99 strain, a strain that has not circulated in the USA since 2005. CONCLUSIONS: Our findings reinforce the hypothesis that WNV has been silently circulating in Brazil for many years.

The analysis of volatiles is an alternative in the routine of quality control of fruits, since the volatile metabolites can change in the presence of microorganisms. A well-established method for extracting volatiles is the solid phase microextraction (SPME). This work aims to define some parameters (fiber coating, conditioning time and fiber exposure time) for an effective extraction. Whole papaya was inserted into a hermetically closed glass bottle and the system was left in conditioning for the volatiles accumulation. Then, extraction by SPME and subsequent desorption in a gas chromatograph was performed. Two fibers were evaluated: a 100 µm polydimethylsiloxane (PDMS) and a 65 µm polydimethylsiloxane/divinylbenzene (PDMS/DVB). The PDMS/DVB fiber extracted the highest number of volatiles. Central composite design (CCD) was performed to assess the effect of papaya conditioning time and the fiber exposure time on the response: number of detectable peaks. Both variables studied and their interactions were significant for the response. ANOVA showed that the quadratic terms and the lack of fit were not significant (p <0.05). A conditioning time of 10 min and exposure time of 30 min was sufficient for the detection of more than 100 compounds.

Resumo Os psilídeos são insetos pequenos que se alimentam do floema e se caracterizam por apresentarem uma pequena faixa de plantas hospedeiras em que se desenvolvem. Este trabalho teve como objetivo prospectar e identificar insetos da superfamília de Psylloidea que ocorrem associados a diferentes biomas no Mato Grosso e que resultou na coleta de 29 gêneros descritos, com 24 espécies identificadas. O Cerrado foi o bioma que apresentou a maior diversidade, seguido pela Amazônia e Pantanal. Comparando com a última lista de psilídeos publicada para o Brasil, em 2012, este artigo contribuiu significativamente para o aumento do conhecimento da biodiversidade de Mato Grosso. Além das espécies registradas para o Mato Grosso (Diaphorina citri, Heteropsylla caldwelli, Pseudophacopteron longicaudatum, Tainarys myracrodruie Trioza struthanthi), também apresentamos novos registros para outros estados brasileiros: Isogonoceraia divergipennis- no Maranhão, Rio Grande do Sul, Rio de Janeiro e Santa Catarina; Heteropsylla caldwelli - em Goiás e São Paulo; Euphalerus clitoriae - no Ceará, Goiás, Pará, Paraná e Santa Catarina; Trioza tabebuiae - em Minas Gerais, São Paulo e Santa Catarina; e Trioza struthanthi em São Paulo.

Abstract Psyllids or jumping plant-lice are small phloem-feeding insects characterised by narrow host ranges, i.e., the plants on which they develop. This work shows the results of a survey of the superfamily Psylloidea in different biomes of Mato Grosso state. In total, 29 described genera and 24 described species are listed. The Cerrado biome showed the highest diversity, followed by the Amazon and Pantanal. Compared with a checklist published for Brazil in 2012, the present paper increases significantly the knowledge on the biodiversity of the state of Mato Grosso. In addition to the species recorded here for the first time from Mato Grosso (Diaphorina citri, Heteropsylla caldwelli, Pseudophacopteron longicaudatum, Tainarys myracrodrui and Trioza struthanthi), we provide also new records for other Brazilian states: Isogonoceraia divergipennis - from Maranhão, Rio Grande do Sul, Rio de Janeiro and Santa Catarina; Heteropsylla caldwelli - from Goiás and São Paulo; Euphalerus clitoriae - from Ceará, Goiás, Pará, Paraná and Santa Catarina; Trioza tabebuiae - from Minas Gerais, São Paulo and Santa Catarina; and Trioza struthanthi from São Paulo.

RESUMO A competência motora (CM) é um atributo associado a comportamentos positivos de saúde; fatores contextuais, parecem desempenhar um papel importante para CM de crianças. O objetivo deste estudo foi comparar a CM de pré-escolares em dois diferentes anos e identificar o efeito de fatores contextuais (tipo de escola e renda). Duas amostras representativas de crianças (3 a 5 anos) da cidade do Recife foram avaliadas em dois momentos: no ano de 2010 (282 crianças; 151 meninos) e 2012 (270 crianças; 148 meninos). A avaliação da CM foi realizada com o Test of Gross Motor Development - 2. A análise dos dados utilizou a Anova Two-Way e a Ancova ajustada por tipo de escola (pública e particular) e renda familiar, foi adotado um p<0.05. Foi identificada uma superioridade nas habilidades locomotoras, de controle de objetos e no quociente motor geral para as crianças analisadas em 2010 e o tipo de escola e renda aumentaram o tamanho do efeito das diferenças nas habilidades de controle de objetos e no quociente motor geral. Podemos concluir que houve uma diminuição na CM da população pré-escolar após dois anos e que as variáveis contextuais tipo de escola e renda são importantes para o desenvolvimento da CM.

ABSTRACT Motor competence (MC) is an attribute associated with positive health behaviors; contextual factors, seems to play a important role for MC of children. The purpose of this study was to compare the MC of preschool children in two different years and to identify the effect of contextual factors (school type and family income). Two representative samples of children (3 to 5 years old) from Recife were assessed in two time periods: 2010 (n= 282 children, 151 boys) and 2012 (n=270 children, 148 boys). MC was assessed using the Test of Gross Motor Development - 2. Data analysis used the Anova Two-Way and the Ancova tests adjusted by type of school (public and private) and family income, a p <0.05 was used. Locomotor skills, object control and overall motor quotient were superior for the children analyzed in 2010. Furthermore, the school type and income increased the effect size in the differences in object control skills and the general motor quotient. We can conclude that there was a decrease in MC of the preschool population after two years and that the contextual variables type of school and family income are important for the development of MC.

Resumo Objetivou-se analisar a associação entre composição corporal e desempenho no teste aeróbico específico do karatê (KSAT). Trata-se de um estudo realizado com treze atletas (6 mulheres) com idade média de 20,7 ± 4,2 anos, afiliados à Federação das Associações de Karatê de Pernambuco, estavam desenvolvendo essa rotina de treinamento durante a temporada de 2018. A massa corporal e a estatura foram medidas. Para medir a composição corporal, o método de densitometria corporal foi utilizado pela técnica de absorciometria por raios-x duplos (DEXA). Teste aeróbico específico de karatê foi usado para verificar o desempenho aeróbico. As análises de associação foram realizadas entre as variáveis de composição corporal e o desempenho do KSAT usando o teste de correlação de Pearson e regressão linear. Todas as análises adotaram uma significância de p <0,05. O desempenho no KSAT foi negativamente correlacionado com a massa gorda total (r = -0,797; p = 0,001) e o percentual de gordura (r = -0,757; p = 0,003). O modelo de regressão linear com maior poder explicativo incluiu gordura total e KSAT (R² ajustado = 0,732; p <0,001) exibiu associação negativa com gordura total (? = -0,21; p <0,001) e o modelo que incluiu% de gordura corporal e KSAT (R² ajustado = 0,708; p = 0,003) foram associados negativamente com% de gordura corporal (? = -22,937; p = 0,001), ambos ajustados por sexo e idade. Existe uma associação negativa entre a massa gorda total, o percentual de gordura corporal e o desempenho aeróbico específico do karatê.

Abstract The aim of this study was to analyze the association between body composition and performance in the karate specific aerobic test (KSAT). This is a study carried out with thirteen athletes (6 females) with a mean age of 20.7 ± 4.2 years, affiliated to the Pernambuco Federation of Karate Associations, were developing this training routine during the 2018 season. Body mass and height were measured. To measure body composition, the body densitometry method was used by the x-ray double-ray absorptiometry (DEXA) technique. Karate Specific Aerobic Test was used to verify aerobic performance. Association analyzes were performed between body composition variables and KSAT performance using Pearson's correlation test and linear regression. All analyzes adopted a significance of p <0.05. KSAT performance was negatively correlated with total fat mass (r = -0.797; p = 0.001) and fat percentage (r = -0.757; p = 0.003). The linear regression model with the highest explanatory power included total fat and KSAT (adjusted R² = 0.732; p <0.001) exhibited a negative association with total fat (? = -0.21; p <0.001), and the model that included % body fat and KSAT (adjusted R² = 0.708; p = 0.003) were negatively associated with% body fat (? = -22.937; p = 0.001), both adjusted for gender and age. There is a negative association between total fat mass, body fat percentage and karate specific aerobic test performance.

Resumo Este estudo teve como objetivo estimar a força propulsora da braçada(FPB) em jovens nadadores do estilo livre e propor uma equação para estimar a força propulsora, considerando variáveis antropométricas e a maturação. Setenta e seis nadadores competitivos [meninos: n = 53; idade = 13,58 ± 1,79; meninas: n = 23; idade = 12,98 ± 1,79] participaram deste estudo. Foram avaliados: altura, massa magra, estatura sentada, comprimento da perna, envergadura, dobra cutânea do tríceps, área muscular do braço (AMB) e maturação. A força propulsiva da braçada foi medida pelo teste de nado atado. Uma regressão linear múltipla (modelo concorrente) foi usada para desenvolver a equação usando as variáveis AMB, envergadura e maturação. O método de Bland – Altman foi utilizado para comparar os valores encontrados entre FPB e força propulsiva estimada (FPE). Houve correlação significativa entre as variáveis FPB e AMB (R² = 0,12; p <0,01), envergadura (R² = 0,21; p <0,01) e maturação (R² = 0,20; p <0,01) para meninos, enquanto os valores das meninas foram: AMB (R² = 0,07; p = 0,20), envergadura (R² = 0,50; p <0,01) e maturação (R² = 0,44; p <0,01). Duas equações lineares foram estabelecidas para predizer a FPB em meninos e meninas. A análise de Bland-Altman mostrou concordância entre FPB e FPE. Em conclusão, a equação é uma ferramenta valiosa para monitorar o treinamento e ajudar a melhorar o desempenho do nadador.

Abstract This study aimed to estimate the propulsive force of the arm (PFA) in young freestyle swimmers and propose an equation to estimate propulsive force, considering anthropometric variables and maturity offset. Seventy-six competitive swimmers [boys: n= 53; age= 13.58 ± 1.79; girls: n= 23; age= 12.98 ± 1.79] participated in this study. Height, lean mass, sitting height, leg length, arm span, triceps skinfold, arm muscle area (AMA), and maturity offset were assessed. The propulsive force of the arm was measured by the tethered swim test. A multiple linear regression (concurrent model) was used to develop the equation using the variables AMA, arm span, and maturity offset. The Bland–Altman method was used to compare the values found between PFA and propulsive force–estimated (PFE). There was a significant correlation between the variables PFA and AMA (R²=0.12; p<0.01), arm span (R²=0.21; p<0.01), and maturity offset (R²=0.20; p<0.01) for boys, whilst in girls theses values were: AMA (R²=0.07;p=0.20), arm span (R²=0.50;p<0.01), and maturity offset (R²=0.44;p<0.01). Two linear equations were established to predict the PFA in boys and girls Bland-Altman analysis showed an agreement between PFA and PFE. In conclusion, the equation is a valuable tool to monitor training and help improve swimmer performance.

Abstract Aims: To analyze the average and individual responses of sleep quality and daytime sleepiness in adolescents after four weeks of strength training. Methods: 19 adolescents with sleep problems recruited in the Federal Institute of Pernambuco, were subject to anthropometric evaluations as well as those for body composition assessment, a 1 repetition maximum test, the sleep parameters (Pittsburgh Sleep Quality Index-PSQI and Epworth Sleepiness Scale-ESS) and were submitted to four weeks of strength-training, performed alternately by segment, two sessions per week, according to recommendations for this population. Results: A decrease in the average PSQI score was observed (10.3±3.3 vs 8.8±4.0; p=0.006), but not in ESS (p>0.05), after intervention. The individual analyses demonstrated that ~63% of adolescents experienced reductions ≥ 3 points in the PSQI and ~58% of them experienced reductions ≥ 3 points in the measure of daytime sleepiness. The prevalence of poor sleep quality and daytime sleepiness reduced from 84.2% to 68.4% and from 52.6% to 31.6%, respectively. The comparisons of high and low responders to exercise training show that adolescents who reduced ≥3 points in the score of a least one sleep parameter presented lower weight, fat mass, and fat percentage (p<0.05). Conclusion: A short-term strength-training program is able to improve global sleep quality, but not daytime sleepiness in adolescents. Furthermore, the changes after training are highly heterogeneous. Further studies are required to better understand the effects of strength training on sleep parameters of adolescents.