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au:Queiroz, Adriana Gonçalves

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Clinical and immunological features of laryngeal cryptococcosis
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Oliveira, Vítor Falcão de

; Taborda, Mariane

; Catarina, Mateus Bach Santa

; Kruschewsky, Wdson Luis Lima

; Rapozo, Marjorie Marini

; Rocha, Thais Queiroz da

; Pagliari, Carla

; Magri, Adriana Satie Gonçalves Kono

; Magri, Marcello Mihailenko Chaves

; Soto, Miriam Nacagami

Revista do Instituto de Medicina Tropical de São Paulo

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2024, Volume 66 elocation e40

Abstract: Pt | Text: Pt | PDF: Pt

ABSTRACT The literature holds few descriptions on immune response findings for laryngeal cryptococcosis. Immunology has been more extensively described in cases involving the central nervous system and the lungs, although many of these studies were conducted in animal models. We aimed to analyze the clinical and immunological characteristics of three patients with laryngeal cryptococcosis. We observed a weak participation of the innate immune response, whereas adaptive immunity showed the predominance of a Th2-type response over a Th1-type response. Most cases occur in male older adults with immunosuppressive conditions, of which HIV infection was absent. Hoarseness configured the main symptom. We found a disease that was restricted to the larynx and possibly the lungs by contiguity. Patients with hoarseness and lesions in nasal endoscopy should be investigated for cryptococcosis by a biopsy of the larynx, including with negative serum cryptococcal antigen. The immunological aspects of our findings of laryngeal involvement resembled those in the most commonly affected systems. models Th2type Thtype Th2 type Th Th1type Th1 conditions absent symptom contiguity antigen systems

https://doi.org/10.1590/s1678-9946202466040

Construção e Validação do Protocolo EmpoderACO Direcionado a Pacientes em Anticoagulação Oral com Varfarina
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Barbosa, Hannah Cardoso

; Torres, Heloisa de Carvalho

; Oliveira, João Antônio de Queiroz

; Santos, Rebeca Priscila de Melo

; Costa, Josiane Moreira da

; Miranda, Leonardo Gonçalves

; Pagano, Adriana Silvina

; Praxedes, Marcus Fernando da Silva

; Martins, Maria Auxiliadora Parreiras

Arquivos Brasileiros de Cardiologia

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2023, Volume 120 N. 6 elocation e20220576

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Resumo Fundamento A varfarina é um anticoagulante oral útil para prevenção de tromboembolismo, embora seja considerado fármaco de alto risco de causar eventos adversos. Considerando os desafios práticos no controle da anticoagulação oral, os pacientes poderiam se beneficiar de estratégias educacionais que visem mudança de comportamento, participação ativa no autocuidado e adesão à farmacoterapia. Objetivo Construir e validar o protocolo EmpoderACO para mudança de comportamento em pacientes em uso de varfarina. Métodos As etapas metodológicas foram: definição de conceitos e domínios do autocuidado, identificação dos objetivos, construção e seleção dos itens, avaliação da validade de conteúdo e pré-teste na população alvo. Resultados Relevância, adequação, clareza e confiabilidade interna dos itens do instrumento foram avaliadas por comitê de juízes multiprofissional pela plataforma web E-surv, obtendo-se média de concordância ≥0,91. A compreensão do instrumento pela população-alvo teve clareza adequada com média de 0,96. Conclusão O EmpoderACO poderá contribuir para qualificar o processo de comunicação entre profissionais e pacientes, melhorar a adesão ao tratamento e os resultados clínicos, podendo ser replicado nos serviços de saúde. tromboembolismo adversos farmacoterapia objetivos préteste pré teste alvo Relevância adequação Esurv, Esurv E surv, surv E-surv obtendose obtendo 091 0 91 ≥0,91 populaçãoalvo 096 96 0,96 clínicos saúde 09 9 ≥0,9 0,9 ≥0, 0, ≥0 ≥

Abstract Background Warfarin is an oral anticoagulant that is very useful in preventing thromboembolism, though it is considered a drug with a high risk of causing adverse events. Considering the practical challenges in controlling oral anticoagulation, the patients on warfarin could benefit from educational strategies aimed at behavioral changes, active participation in self-care, and adherence to drug therapy. Objective The aim was to construct and validate the EmpoderACO protocol for behavioral changes in warfarin patients. Methods The methodological steps were: definition of concepts and domains of self-care, identification of objectives, construction and selection of items, assessment of content validity, and pre-test in the target population. Results Relevance, adequacy, clarity, and internal reliability of the instrument’s items were assessed by a multidisciplinary judges committee (JC) through the E-surv web platform, obtaining an average agreement of ≥0.91. The understanding of the instrument measured by the target population revealed adequate clarity with a coefficient average of 0.96. Conclusion EmpoderACO can aid in qualifying the communication process between medical professionals and patients, as well as in improving adherence to both treatment and clinical outcomes, and can be replicated in healthcare settings. thromboembolism events anticoagulation selfcare, selfcare self care, care self-care therapy objectives validity pretest pre test Relevance adequacy instruments s JC (JC Esurv E surv platform 091 0 91 ≥0.91 096 96 0.96 outcomes settings 09 9 ≥0.9 0.9 ≥0. 0. ≥0 ≥

https://doi.org/10.36660/abc.20220576

Construção coletiva do entendimento de lazer na saúde mental territorial por terapeutas ocupacionais: um estudo qualitativo ocupacionais
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Queiroz, Adriana Gonçalves

; Morrison, Rodolfo

Cadernos Brasileiros de Terapia Ocupacional

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2023, Volume 31 elocation e3546

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Resumo Introdução O lazer é considerado uma ocupação presente nos estudos, avaliações e intervenções da terapia ocupacional. Na América Latina, há poucos trabalhos que se dedicam à construção do entendimento dos profissionais ou suas percepções acerca do lazer. São ainda mais escassos os estudos que correlacionam com a ciência ocupacional o entendimento destes profissionais sobre tal ocupação dentro do campo da saúde mental. Objetivo Conhecer o entendimento de lazer por terapeutas ocupacionais atuantes na saúde mental de serviços públicos substitutivos ao modelo psiquiátrico asilar. Metodologia Esta pesquisa foi qualitativa, descritiva e exploratória, com desenho transversal. Foi utilizado grupo focal e análise temática da informação advinda do processo de construção coletiva de terapeutas ocupacionais, atuantes em serviços públicos de base territorial de urgência em saúde mental, da cidade de Belo Horizonte, Minas Gerais, Brasil, sobre seus entendimentos quanto ao lazer. Resultados Três categorias emergiram: (a) Lazer - prazer; (b) Lazer - livre escolha; e (c) Lazer - transcendência da dureza. Conclusão O lazer é uma ocupação importante para terapeutas ocupacionais atuantes em saúde mental no território; pode encontrar ressonância em práticas que fomentam a contratualidade e autonomia, especialmente ao se aliar aos princípios da ciência ocupacional de valorização do fazer humano em suas variáveis formas de vivenciá-lo. Acredita-se que esta pesquisa possa auxiliar terapeutas ocupacionais e cientistas da ocupação no entendimento do lazer contemporâneo, em diálogo com o cuidado no território e posicionamento antimanicomial. Latina asilar qualitativa exploratória transversal Horizonte Gerais Brasil emergiram (a prazer b (b escolha c (c dureza autonomia vivenciálo. vivenciálo vivenciá lo. lo vivenciá-lo Acreditase Acredita contemporâneo antimanicomial

Abstract Introduction Leisure is considered an occupation present in studies, evaluations and interventions of occupational therapy. In Latin America, there are few works dedicated to building the understanding of professionals or their perceptions about leisure. There are even fewer studies that correlate the understanding of these professionals with occupational science on this occupation within the field of mental health. Objective To know the understanding of leisure by occupational therapists working in the mental health of public services that substitute the asylum psychiatric model. Methodology : This research was qualitative, descriptive and exploratory, with a cross-sectional design. A focus group and thematic analysis of information arising from the process of collective construction of occupational therapists, working in public services with a territorial base of urgency in mental health, in the city of Belo Horizonte, Minas Gerais, Brazil, on their understandings regarding leisure were used. Results Three categories emerged: (a) Leisure - pleasure; (b) Leisure - free choice; and (c) Leisure - transcendence of hardness. Conclusion Leisure is an important occupation for occupational therapists working in mental health in the territory; may find resonance in practices that encourage contractuality and autonomy, especially when combined with the principles of occupational science of valuing human doing in its variable ways of experiencing it. It is believed that this research can help occupational therapists and occupation scientists in understanding contemporary leisure, in dialogue with care in the territory and anti-asylum positioning. therapy America model qualitative exploratory crosssectional cross sectional design Horizonte Gerais Brazil used emerged (a pleasure b (b choice c (c hardness autonomy it antiasylum anti positioning

https://doi.org/10.1590/2526-8910.ctoao265035461

SECOND BRAZILIAN CONSENSUS ON THE MANAGEMENT OF CROHN’S DISEASE IN ADULTS: A CONSENSUS OF THE BRAZILIAN ORGANIZATION FOR CROHN’S DISEASE AND COLITIS (GEDIIB) CROHNS CROHN S ADULTS GEDIIB (GEDIIB
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IMBRIZI, Marcello

; BAIMA, Júlio Pinheiro

; AZEVEDO, Matheus Freitas Cardoso de

; ANDRADE, Adriana Ribas

; QUEIROZ, Natália Souza Freitas

; CHEBLI, Júlio Maria Fonseca

; CHEBLI, Liliane Andrade

; ARGOLLO, Marjorie Costa

; SASSAKI, Ligia Yukie

; PARRA, Rogerio Serafim

; QUARESMA, Abel Botelho

; VIEIRA, Andrea

; DAMIÃO, Adérson Omar Mourão Cintra

; MORAES, Antonio Carlos da Silva

; FLORES, Cristina

; ZALTMAN, Cyrla

; VILELA, Eduardo Garcia

; MORSOLETTO, Eloá Marussi

; GONÇALVES FILHO, Francisco de Assis

; PENNA, Francisco Guilherme Cancela e

; SANTANA, Genoile Oliveira

; ZABOT, Gilmara Pandolfo

; PARENTE, José Miguel Luz

; COSTA, Marcia Henriques de Magalhães

; ZERÔNCIO, Marco Antonio

; MACHADO, Marta Brenner

; CASSOL, Ornella Sari

; KOTZE, Paulo Gustavo

; FRÓES, Renata de Sá Brito

; MISZPUTEN, Sender Jankiel

; AMBROGINI JUNIOR, Orlando

; SAAD-HOSSNE, Rogério

; COY, Cláudio Saddy Rodrigues

Arquivos de Gastroenterologia

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2022, Volume 59 Suppl. 1 Pages 20 - 50

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO Contexto: A doença inflamatória intestinal (DII) é uma doença imunomediada que inclui a doença de Crohn (DC) e a retocolite ulcerativa. A DC é caracterizada por um envolvimento intestinal transmural da boca ao ânus com sintomas recorrentes e remitentes que podem levar a danos intestinais progressivos e incapacidade ao longo do tempo. Objetivo: Orientar os tratamentos médicos mais seguros e eficazes de adultos com DC. Métodos: Este consenso foi desenvolvido por autores que representam gastroenterologistas e cirurgiões brasileiros especialistas em doenças colorretais (GEDIIB, Organização Brasileira de Doença de Crohn e Colite). Uma revisão sistemática das evidências mais recentes foi realizada para apoiar as recomendações/declarações. Todas as recomendações e declarações incluídas foram endossadas em um painel Delphi modificado pelas partes interessadas e especialistas em DII com uma concordância de pelo menos 80% ou mais. Resultados e conclusão: As recomendações médicas (intervenções farmacológicas e não farmacológicas) foram mapeadas de acordo com o estágio de tratamento e gravidade da doença em três domínios: manejo e tratamento (intervenções medicamentosas e cirúrgicas), critérios para avaliar a eficácia do tratamento médico, e acompanhamento/monitoramento do paciente após o tratamento inicial. O consenso é direcionado a clínicos gerais, gastroenterologistas e cirurgiões interessados em tratar e gerenciar adultos com DC e apoia a tomada de decisões de companhias de seguro de saúde, agências reguladoras e líderes ou administradores de instituições de saúde. Contexto (DII (DC ulcerativa tempo Objetivo Métodos GEDIIB, GEDIIB (GEDIIB Colite. Colite . Colite) recomendaçõesdeclarações recomendações/declarações 80 conclusão intervenções domínios cirúrgicas, cirúrgicas , cirúrgicas) médico acompanhamentomonitoramento acompanhamento monitoramento inicial gerais saúde 8

ABSTRACT Background: Inflammatory bowel disease (IBD) is an immune-mediated disorder that includes Crohn’s disease (CD) and ulcerative colitis. CD is characterized by a transmural intestinal involvement from the mouth to the anus with recurrent and remitting symptoms that can lead to progressive bowel damage and disability over time. Objective: To guide the safest and effective medical treatments of adults with CD. Methods: This consensus was developed by stakeholders representing Brazilian gastroenterologists and colorectal surgeons (Brazilian Organization for Crohn's disease and Colitis (GEDIIB)). A systematic review of the most recent evidence was conducted to support the recommendations/statements. All included recommendations and statements were endorsed in a modified Delphi panel by the stakeholders and experts in IBD with an agreement of at least 80% or greater consensus rate. Results and conclusion: The medical recommendations (pharmacological and non-pharmacological interventions) were mapped according to the stage of treatment and severity of the disease in three domains: management and treatment (drug and surgical interventions), criteria for evaluating the effectiveness of medical treatment, and follow-up/patient monitoring after initial treatment. The consensus is targeted towards general practitioners, gastroenterologists, and surgeons interested in treating and managing adults with CD and supports the decision-making of health insurance companies, regulatory agencies, and health institutional leaders or administrators. Background (IBD immunemediated immune mediated Crohns Crohn s (CD colitis time Objective Methods GEDIIB. GEDIIB . (GEDIIB)) recommendationsstatements recommendations/statements 80 rate conclusion pharmacological nonpharmacological non interventions domains drug interventions, , followup/patient followuppatient follow up/patient up patient practitioners decisionmaking decision making companies agencies administrators (GEDIIB) 8 followup uppatient (GEDIIB

https://doi.org/10.1590/s0004-2803.2022005s1-02

SECOND BRAZILIAN CONSENSUS ON THE MANAGEMENT OF ULCERATIVE COLITIS IN ADULTS: A CONSENSUS OF THE BRAZILIAN ORGANIZATION FOR CROHN’S DISEASE AND COLITIS (GEDIIB) ADULTS CROHNS CROHN S GEDIIB (GEDIIB
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BAIMA, Júlio Pinheiro

; IMBRIZI, Marcello

; ANDRADE, Adriana Ribas

; CHEBLI, Liliane Andrade

; ARGOLLO, Marjorie Costa

; QUEIROZ, Natália Sousa Freitas

; AZEVEDO, Matheus Freitas Cardoso de

; VIEIRA, Andrea

; COSTA, Marcia Henriques de Magalhães

; FRÓES, Renata de Sá Brito

; PENNA, Francisco Guilherme Cancela e

; QUARESMA, Abel Botelho

; DAMIÃO, Aderson Omar Mourão Cintra

; MORAES, Antonio Carlos da Silva

; SANTOS, Carlos Henrique Marques dos

; FLORES, Cristina

; ZALTMAN, Cyrla

; VILELA, Eduardo Garcia

; MORSOLETTO, Eloá

; GONÇALVES FILHO, Francisco de Assis

; SANTANA, Genoile Oliveira

; ZABOT, Gilmara Pandolfo

; PARENTE, José Miguel Luz

; SASSAKI, Ligia Yukie

; ZERÔNCIO, Marco Antonio

; MACHADO, Marta Brenner

; CASSOL, Ornella Sari

; KOTZE, Paulo Gustavo

; PARRA, Rogerio Serafim

; MISZPUTEN, Sender Jankiel

; COY, Cláudio Saddy Rodrigues

; AMBROGINI JUNIOR, Orlando

; CHEBLI, Júlio Maria Fonseca

; SAAD-HOSSNE, Rogério

Arquivos de Gastroenterologia

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2022, Volume 59 Suppl. 1 Pages 51 - 84

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO Contexto: As doenças inflamatórias intestinais são doenças imunomediadas que incluem a doença de Crohn (DC) e a retocolite ulcerativa (RCU). A RCU é uma doença progressiva que acomete a mucosa colorretal causando sintomas debilitantes levando a alta morbidade e incapacidade laboral. Como consequência da inflamação crônica do cólon, a RCU também está associada a um risco aumentado de câncer colorretal. Objetivo: Este consenso visa fornecer orientações sobre o manejo médico mais eficaz de pacientes adultos com RCU. Métodos: As recomendações do consenso foram desenvolvidas por gastroenterologistas e cirurgiões colorretais referências no Brasil (membros da Organização Brasileira para Doença de Crohn e Colite [GEDIIB]). Uma revisão sistemática, incluindo as evidências mais recentes, foi conduzida para apoiar as recomendações. Todas as recomendações foram endossadas pelas partes interessadas/especialistas em doença inflamatória intestinal usando um Painel Delphi modificado. O nível de concordância para alcançar consenso foi de 80% ou mais. Resultados e conclus ão: As recomendações médicas (farmacológicas e não farmacológicas) foram mapeadas de acordo com o estágio de tratamento e gravidade da doença em três domínios: manejo e tratamento (intervenções medicamentosas e cirúrgicas), critérios para avaliar a eficácia do tratamento médico, e acompanhamento/monitoramento do paciente após o tratamento inicial. O consenso foi direcionado a clínicos gerais, gastroenterologistas e cirurgiões que tratam pacientes com RCU e apoia os processos de tomada de decisão por companhias de seguro de saúde, agências reguladoras, líderes institucionais de saúde e administradores. Contexto DC (DC . (RCU) laboral cólon Objetivo Métodos membros GEDIIB. GEDIIB [GEDIIB]) sistemática recentes interessadasespecialistas interessadas especialistas modificado 80 ão farmacológicas domínios intervenções cirúrgicas, cirúrgicas , cirúrgicas) acompanhamentomonitoramento acompanhamento monitoramento inicial gerais reguladoras administradores (RCU [GEDIIB] 8 [GEDIIB

ABSTRACT Background: Inflammatory bowel diseases are immune-mediated disorders that include Crohn’s disease (CD) and ulcerative colitis (UC). UC is a progressive disease that affects the colorectal mucosa causing debilitating symptoms leading to high morbidity and work disability. As a consequence of chronic colonic inflammation, UC is also associated with an increased risk of colorectal cancer. Objective: This consensus aims to provide guidance on the most effective medical management of adult patients with UC. Methods: A consensus statement was developed by stakeholders representing Brazilian gastroenterologists and colorectal surgeons (Brazilian Organization for Crohn’s Disease and Colitis [GEDIIB]). A systematic review including the most recent evidence was conducted to support the recommendations and statements. All recommendations/statements were endorsed using a modified Delphi Panel by the stakeholders/experts in inflammatory bowel disease with at least 80% or greater consensus. Results and conclusion: The medical recommendations (pharmacological and non-pharmacological) were mapped according to the stage of treatment and severity of the disease onto three domains: management and treatment (drug and surgical interventions), criteria for evaluating the effectiveness of medical treatment, and follow-up/patient monitoring after initial treatment. The consensus targeted general practitioners, gastroenterologists and surgeons who manage patients with UC, and supports decision-making processes by health insurance companies, regulatory agencies, health institutional leaders, and administrators. Background immunemediated immune mediated Crohns Crohn s CD (CD . (UC) disability inflammation cancer Objective Methods GEDIIB. GEDIIB [GEDIIB]) statements recommendationsstatements stakeholdersexperts experts 80 conclusion pharmacological nonpharmacological non non-pharmacological domains drug interventions, interventions , interventions) followup/patient followuppatient follow up/patient up patient practitioners decisionmaking decision making companies agencies leaders administrators (UC [GEDIIB] 8 followup uppatient [GEDIIB

https://doi.org/10.1590/s0004-2803.2022005s1-03

Mães acadêmicas: equilibrando os papéis de mães e pesquisadoras
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Pontes, Tatiana Barcelos

; Alves, Aline Teixeira

; Celeste, Letícia Correa

; Bernardo, Lilian Dias

; Queiroz, Adriana Gonçalves

; Poletto, Marina

; Njelesani, Janet

Cadernos Brasileiros de Terapia Ocupacional

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dec 2019, Volume 27 N. 4 Pages 687 - 690

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

https://doi.org/10.4322/2526-8910.ctoed2704 902 downloads

Adaptação transcultural do instrumento Autism Classification System of Functioning: Social Communication (ACSF: SC) para uso no Brasil
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Eloi, Débora Santana

; Mareco, Kesia Kelen Gonçalves

; Queiroz, Adriana Gonçalves

; Lage, Carla Ribeiro

; Galvão, Cecília Pletschette

; Araújo, Clarice Ribeiro Soares

; Araújo Costa, Samara de

; Cardoso, Ana Amélia

Cadernos Brasileiros de Terapia Ocupacional

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jun 2019, Volume 27 N. 2 Pages 293 - 301

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Resumo Objetivo Realizar a adaptação transcultural do instrumento: Autism Classification System of functioning: Social communication (ACSF: SC), que permite classificar o nível da função da comunicação social de crianças com transtorno do espectro autista (TEA), para uso no Brasil. Método Estudo metodológico de adaptação transcultural do instrumento ACSF: SC. Foram seguidas recomendações para adaptação transcultural de protocolos da área da saúde, que incluiu a permissão assegurada, tradução, tradução reversa, revisão e painel de especialistas. Resultados A avaliação dos especialistas evidenciou que a versão traduzida do instrumento ACSF: SC foi bem compreendida conceitualmente e adequada culturalmente, com 48 frases apresentando índice de concordância maior que 90% quanto à equivalência conceitual e cultural. Frases que não obtiveram níveis adequados de concordância foram revisadas, conforme sugestões dos especialistas. Conclusão A equivalência entre o instrumento ACSF: SC original e a tradução está garantida, obtendo índice de concordância acima de 90%. O instrumento estará apto para uso clínico em breve, após validação de suas propriedades de medida.

Abstract Objective To conduct a cross-cultural adaptation of the instrument: Autism Classification System of functioning: Social communication (ACSF: SC), which allows clinicians to classify the level of the social communication function of children with autism spectrum disorder (ASD), for its use in Brazil. Method Methodological study of cross-cultural adaptation of the instrument ACSF: SC. We followed recommendations for transcultural adaptation of health protocols, which included assured permission, translation, reverse translation, review, and panel of experts. Results The evaluation of the experts revealed that the translated version of the ACSF: SC instrument was well understood conceptually and culturally adequate, with 48 sentences presenting a concordance index greater than 90% regarding conceptual and cultural equivalence. Phrases that did not achieve adequate levels of agreement were reviewed, according to experts’ suggestions. Conclusion Equivalence between the original ACSF: SC and its translated into Brazilian Portuguese is guaranteed, as it reached a concordance index above 90%. The Brazilian version of the ACSF: SC will be suitable for clinical use once its psychometrics properties are established.

https://doi.org/10.4322/2526-8910.ctoao1227 1631 downloads

Increased serum gastrin in patients with different clinical forms of Chagas disease coinfected with Helicobacter pylori
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Sousa, Jacqueline Batista

; Etchebehere, Renata Margarida

; Queiroz, Dulciene Maria de Magalhães

; Fonseca, Fernanda Machado

; Batista, Bianca Bontempi

; Junqueira, Iracema Saldanha

; Camilo, Sílvia Maria Perrone

; Oliveira, Adriana Gonçalves de

Revista do Instituto de Medicina Tropical de São Paulo

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2019, Volume 61 elocation e7

Abstract: En | Text: En | PDF: En

ABSTRACT Trypanosoma cruzi and Helicobacter pylori (HP) are pathogens that cause chronic diseases and have been associated with hypergastrinemia. The aim of this study was to evaluate the fasting gastrin levels in patients with different clinical forms of Chagas disease (CD), coinfected or not by HP. The enrolled individuals were outpatients attending at the university hospital. HP infection was assessed by serology and 13 C-urea breath test. Fasting serum gastrin concentration was measured by chemiluminescence assay. Gastric endoscopic and histological features were also evaluated. Associations between CD and serum gastrin level were evaluated in a logistical model, adjusting for age, gender and HP status. A total of 113 patients were evaluated (45 with Chagas disease and 68 controls). In the multivariate analysis, increasing serum gastrin levels (OR= 1.02; 95% CI= 1.01-1.12), increasing age (OR= 1.05; 95% CI= 1.02 - 1.09) and HP-positive status (OR = 2.88; 95% CI = 1.10 - 7.51) remained independently associated with CD. The serum gastrin levels were significantly higher in the group of patients with the cardiodigestive form ( P = 0.03) as well as with digestive form ( P = <0.001) of Chagas disease than in the controls. In conclusion, patients with cardiodigestive and digestive clinical forms of CD have increased basal serum gastrin levels in comparison with controls. Moreover, we also demonstrated that H. pylori coinfection contributes to the hypergastrinemia shown in CD.

https://doi.org/10.1590/s1678-9946201961007 1988 downloads

Enhanced single-cell oil production by cold shock in cyanobacterial cultures
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Queiroz, Maria Isabel

; Maroneze, Mariana Manzoni

; Manetti, Adriana Gonçalves da Silva

; Vieira, Juliana Guerra

; Zepka, Leila Queiroz

; Jacob-Lopes, Eduardo

Ciência Rural

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2018, Volume 48 N. 11 elocation e20180366

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO: O objetivo deste trabalho foi avaliar o aumento da produção de óleos unicelulares por Aphanothece microscopica Nägeli utilizando água residuária de processamento de laticínios como meio de cultura. O estudo concentrou-se na (i) otimização da temperatura para produção de biomassa, (ii) aplicação de choque frio para induzir a biossíntese de lipídios e (iii) determinação do perfil de ácidos graxos sob diferentes condições. Os resultados indicam que a temperatura de 20°C foi a melhor condição em termos de parâmetros cinéticos, atingindo produtividades de biomassa de 160,25mg/L.h. Nesta condição também foi observado um teor lipídico de 12,65%, o que resultou em uma produtividade lipídica de 20,27mg/L.h. Além disso, o choque frio de 0°C foi o mais eficiente para aumentar o conteúdo lipídico intracelular, que atingiu 28,4% em peso seco. Os choques frios também mostraram influência na saturação da composição de ácidos graxos, em que os ácidos graxos saturados diminuíram, e os ácidos graxos monoinsaturados e poli-insaturados ampliaram com o aumento da aplicação de frio. Assim, o uso de choques frios indica ser uma condição chave para melhorar as perspectivas de produção eficiente de óleos unicelulares.

ABSTRACT: The aim of this research was to evaluate the enhanced single-cell oil production by cold shock in Aphanothece microscopica Nägeli using dairy processing wastewater as culture medium. The study focused on (i) temperature optimization for biomass production, (ii) cold shock application to induce lipids biosynthesis and (iii) determination of fatty acids profile under different conditions. Results indicated that temperature of 20°C was the best condition in terms of kinetics parameter, reaching biomass productivities of 160.25mg/L.h. Under these conditions, a lipid content of 12.65% was also observed, resulting in a lipid productivity of 20.27mg/L.h. Additionally, the 0°C cold shock was the most efficient in increasing intracellular lipid content, reaching 28.4% in dry weight. Cold shocks also showed influence on the saturation of fatty acid composition, where the saturated fatty acids decreased, and the monounsaturated and polyunsaturated fatty acids increased by increasing the cold application. Thus, the use of cold shocks indicates to be a key condition for improving the prospects of efficient single-cell oils production.

https://doi.org/10.1590/0103-8478cr20180366 799 downloads

10.

Brazilian guidelines for the clinical management of paracoccidioidomycosis
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Shikanai-Yasuda, Maria Aparecida

; Mendes, Rinaldo Pôncio

; Colombo, Arnaldo Lopes

; Queiroz-Telles, Flávio de

; Kono, Adriana Satie Gonçalves

; Paniago, Anamaria M. M

; Nathan, André

; Valle, Antonio Carlos Francisconi do

; Bagagli, Eduardo

; Benard, Gil

; Ferreira, Marcelo Simão

; Teixeira, Marcus de Melo

; Silva-Vergara, Mario León

; Pereira, Ricardo Mendes

; Cavalcante, Ricardo de Souza

; Hahn, Rosane

; Durlacher, Rui Rafael

; Khoury, Zarifa

; Camargo, Zoilo Pires de

; Moretti, Maria Luiza

; Martinez, Roberto

Revista da Sociedade Brasileira de Medicina Tropical

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sep 2017, Volume 50 N. 5 Pages 715 - 740

Abstract: En | Text: En | PDF: En

Abstract Paracoccidioidomycosis is a systemic fungal disease occurring in Latin America that is associated with rural environments and agricultural activities. However, the incidence and prevalence of paracoccidiodomycosis is underestimated because of the lack of compulsory notification. If paracoccidiodomycosis is not diagnosed and treated early and adequately, the endemic fungal infection could result in serious sequelae. While the Paracoccidioides brasiliensis ( P. brasiliensis ) complex has been known to be the causal agent of paracoccidiodomycosis, a new species, Paracoccidioides lutzii ( P. lutzii ), has been reported in Rondônia, where the disease has reached epidemic levels, and in the Central West and Pará. Accurate diagnoses and availability of antigens that are reactive with the patients’ sera remain significant challenges. Therefore, the present guidelines aims to update the first Brazilian consensus on paracoccidioidomycosis by providing evidence-based recommendations for bedside patient management. This consensus summarizes etiological, ecoepidemiological, molecular epidemiological, and immunopathological data, with emphasis on clinical, microbiological, and serological diagnosis and management of clinical forms and sequelae, as well as in patients with comorbidities and immunosuppression. The consensus also includes discussion of outpatient treatments, severe disease forms, disease prevalence among special populations and resource-poor settings, a brief review of prevention and control measures, current challenges and recommendations.

https://doi.org/10.1590/0037-8682-0230-2017 10753 downloads

11.

Utilização do dispositivo intravaginal de progesterona, em até três usos, para inseminação artificial em tempo fixo de fêmeas Bos indicus
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MEDALHA, Adriana Gonçalves

; SOUZA, Maria Inês Lenz

; SOUZA, Albert Schiaveto de

; SÁ FILHO, Ocilon Gomes

; QUEIROZ, Vanessa Lopes Dias

; COSTA FILHO, Luiz Carlos César da

Revista Brasileira de Saúde e Produção Animal

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jun 2015, Volume 16 N. 2 Pages 458 - 469

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Objetivou-se avaliar o efeito do número de utilizações (até três) do CIDRÒ, manifestação de estro e ordem de parição sobre a taxa de prenhez em vacas Bos indicus. Utilizou-se 1176 vacas pluríparas e primíparas, lactantes, 30 a 60 dias pós-parto. Os grupos receberam CIDRÒ de primeiro, segundo ou terceiro usos, mais 2,0 mg de benzoato de estradiol no dia de introdução (D0) e, 500 μg de dinoprost trometamina, 300 UI de eCG e 0,6 mg de cipionato de estradiol na retirada dos dispositivos (D8), com a IATF 48 horas após (D10). Em 30 dias realizou-se o diagnóstico de gestação (D40). Analisaram-se as variáveis pelo PROC LOGISTIC (SAS) e pela regressão logística binária, considerando-se significância quando p < 0,05. A quantidade de usos do implante influenciou a taxa de gestação (1 uso < 2 usos – p<0,05; 1 uso < 3 usos – p < 0,01; 2 usos = 3 usos; p > 0,1), de 44,8%, 51,1% e 51,1% para CIDRÒ 1, 2 e 3 usos, respectivamente, bem como a ordem de parição (pluríparas 50,85% > primíparas 34,48%; p<0,01), e manifestação de estro (com estro 44,02% > sem estro 33,06%; p<0,01). O CIDRÒ pode ser utilizado por até três vezes, mas as taxas de gestação mostram-se mais baixas no primeiro uso em relação ao segundo e terceiro usos e com melhores resultados em pluríparas e com manifestação de estro.

The objective was to evaluate the effect of the number of utilizations of CIDRÒ, estrus manifestation and bearing order on the pregnancy rate in Bos indicus. We used in 1176 lactating multiparous and primiparous cows, 30 to 60 days post-partum. The groups received first, second- or third- use CIDRÒ, plus 2,0mg of estradiol benzoate (EB) when the device was introduced (D0), and 500 μg of dinoprost trometamin, 300 UI of eCG and 0,6 mg of estradiol cypionate at the device withdrawal (D8), with FTAI 48 hours after (D10). At 30 days realized the pregnancy diagnosis (D40). The variables were analyzed by PROC LOGISTIC (SAS) and binary logistic regression, and the difference was statistically significant when p<0.05. The amount of implant uses influenced the pregnancy rate (1 use < 2 uses – p < 0.05; 1 use < 3 uses – p<0.01; 2 uses = 3 uses; p>0.1); of 44.8%, 51.1% and 51.1% for CIDRÒ 1, 2 and 3 uses, respectively; as bearing order (multiparous 50.85% > primiparous 34.48%; p<0.01) and estrus manifestation (estrus 44.02% > no estrus 33.06%; p<0.01). CIDRÒ can be utilized up to three times, but the pregnancy rate showed lower for the first-use in relation to second and third uses and biggest results in multiparous and with estrus manifestation.

https://doi.org/10.1590/S1519-99402015000200020 5861 downloads

12.

Histological and endoscopic features of the stomachs of patients with Chagas disease in the era of Helicobacter pylori
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Fonseca, Fernanda Machado

; Etchebehere, Renata Margarida

; Queiroz, Dulciene Maria de Magalhães

; Rocha, Andreia Maria Camargos

; Junqueira, Iracema Saldanha

; Fonseca, Daurin Narciso da

; Rodrigues, Aline Libério Braga

; Crema, Eduardo

; de Oliveira, Adriana Gonçalves

Revista da Sociedade Brasileira de Medicina Tropical

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dec 2014, Volume 47 N. 6 Pages 739 - 746

Abstract: En | Text: En | PDF: En

Introduction Most studies that have evaluated the stomachs of patients with Chagas disease were performed before the discovery of Helicobacter pylori and used no control groups. This study compared the gastric features of chagasic and non-chagasic patients and assessed whether gastritis could be associated with Chagas disease. Methods Gastric biopsy samples were taken from patients who underwent endoscopy for histological analysis according to the Updated Sydney System. H. pylori infection was assessed by histology, 16S ribosomal ribonucleic acid (rRNA) polymerase chain reaction (PCR), serology and the 13C-urea breath test. Patients were considered H. pylori-negative when all of these diagnostic tests were negative. Clinical and socio-demographic data were obtained by reviewing medical records and using a questionnaire. Results The prevalence of H. pylori infection (70.3% versus 71.7%) and chronic gastritis (92.2% versus 85%) was similar in the chagasic and non-chagasic groups, respectively; such as peptic ulcer, atrophy and intestinal metaplasia. Gastritis was associated with H. pylori infection independent of Chagas disease in a log-binomial regression model. However, the chagasic H. pylori-negative patients showed a significantly higher grade of mononuclear (in the corpus) and polymorphonuclear (PMN) (in the antrum) cell infiltration. Additionally, the patients with the digestive form of Chagas disease showed a significantly lower prevalence of corpus atrophy than those with other clinical forms. Conclusions The prevalence of H. pylori infection and of gastric histological and endoscopic features was similar among the chagasic and non-chagasic patients. Additionally, this is the first controlled study to demonstrate that H. pylori is the major cause of gastritis in patients with Chagas disease.

https://doi.org/10.1590/0037-8682-0180-2014 2342 downloads

13.

Influência da temperatura na remoção de fosfato por microalgas em biorreatores heterotróficos
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Vieira, Juliana Guerra

; Jacob-Lopes, Eduardo

; Manetti, Adriana Gonçalves da Silva

; Queiroz, Maria Isabel

Revista Ambiente & Água

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jun 2012, Volume 7 N. 2 Pages 101 - 109

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Neste trabalho, a eficiência da cianobactéria Aphanothece microscopica Nägeli em remover heterotroficamente fosfato total dissolvido do efluente do processamento de laticínios foi avaliada. Os experimentos foram conduzidos em biorreatores operados descontinuamente, alimentados com 4,5 L de efluente. As condições experimentais foram: concentração celular inicial de 0,2 g/L, pH ajustado a 7,6, reator isotérmico operando em temperaturas de 10, 20 e 30ºC, ausência de luminosidade e aeração contínua de 1 VVM. Os resultados mostraram que a remoção de fosfato é fortemente dependente da temperatura do processo. A cianobactéria A. microscopica Nägelifoi efetiva na remoção de fosfato,alcançando taxas de remoção de 3,77 mg/L.h, que refletiram em conversões de 98,4% em tempos de detenção hidráulica de 24 h.

The removal of total dissolved phosphate by the cyanobacteria Aphanothece microscopica Nägeli cultivated heterotrophically in dairy processing wastewater was investigated in this work. The experiments were carried out in bioreactors operating in a batch mode, fed with 4.5 L of wastewater. The experimental conditions were as follows: initial cell concentration of 0.2 g/L, pH adjusted to 7.6, isothermal reactor operating at temperatures of 10, 20 and 30ºC with absence of light and continuous aeration of 1 VVM. The results showed that phosphate removal is strongly dependent on process temperature. A. microscopica Nägeli was effective in the removal phosphate achieving removal rates of 3.77 mg/L.h, which reflected in the conversion of 98.4% in hydraulic detention times of 24 h.

https://doi.org/10.4136/ambi-agua.856 1395 downloads Cited 1 time in SciELO

14.

Seroprevalence of Helicobacter pylori infection in chagasic and nonchagasic patients from the same geographical region of Brazil
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Fonseca, Fernanda Machado

; Queiroz, Dulciene Maria de Magalhães

; Rocha, Andreia Maria Camargos

; Prata, Aluízio

; Crema, Eduardo

; Rodrigues Junior, Virmondes

; Ramirez, Luiz Eduardo

; Oliveira, Adriana Gonçalves de

Revista da Sociedade Brasileira de Medicina Tropical

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apr 2012, Volume 45 N. 2 Pages 194 - 198

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

INTRODUÇÃO: No presente estudo, foi comparada a soroprevalência da infecção por Helicobacter pylori entre os indivíduos chagásicos e não-chagásicos, bem como entre subgrupos de chagásicos com as formas clínicas indeterminada, cardíaca, digestiva e cardiodigestiva. MÉTODOS: Os indivíduos avaliados eram provenientes da região do Triângulo Mineiro, Minas Gerais, Brasil. Foram realizados testes sorológicos convencionais para diagnóstico da infecção pelo T. cruzi e os chagásicos foram classificados de acordo com a forma clínica. O diagnóstico de infecção por H. pylori foi estabelecido pela detecção de anticorpos IgG específicos utilizando-se um kit comercial de ELISA. RESULTADOS: A prevalência da infecção por H. pylorifoi 77,1% (239/310) no grupo de pacientes chagásicos e 69,1% (168/243) no grupo de não-chagásicos. Esta diferença foi estatisticamente significativa mesmo após ajuste para idade e sexo (OR = 1,57; 95% CI, 1,02-2,42; p = 0,04) na análise multivariada. A prevalência da infecção aumentou de acordo com a idade no grupo não-chagásicos (p = 0,007, χ2 for trend) mas este aumento não foi observado no grupo dos chagásicos (p = 0,15, χ2 for trend). Não houve associação da infecção por H. pylori com a forma digestiva ou com qualquer outra forma clínica da doença de Chagas (p = 0,27). CONCLUSÕES: Foi demonstrado que pacientes chagásicos apresentam maior prevalência da infecção por H. pylori quando comparados com não-chagásicos, independente da forma clínica da doença. Os fatores que contribuem para a frequente co-infecção Helicobacter pylori e Trypanosoma cruzi, bem como seus efeitos na evolução clínica das doenças associadas devem ser melhor estudados.

INTRODUCTION: In this study, we evaluated the seroprevalence of Helicobacter pylori infection among chagasic and non-chagasic subjects as well as among the subgroups of chagasic patients with the indeterminate, cardiac, digestive, and cardiodigestive clinical forms. METHODS: The evaluated subjects were from the Triângulo Mineiro region, Minas Gerais, Brazil. Chagasic patients showed positive reactions to the conventional serological tests used and were classified according to the clinical form of their disease. Immunoglobulin G antibodies specific to H. pylori were measured using a commercial enzyme-linked immunosorbent assay kit. RESULTS: The overall H. pylori prevalence was 77.1% (239/310) in chagasic and 69.1% (168/243) in non-chagasic patients. This difference was statistically significant even after adjustment for age and sex (odds ratio = 1.57; 95% confidence interval, 1.02-2.42; p = 0.04) in multivariate analysis. The prevalence of infection increased with age in the non-chagasic group (p = 0.007, χ2 for trend), but not in the chagasic group (p = 0.15, χ2 for trend). H. pylori infection was not associated with digestive or other clinical forms of Chagas disease (p = 0.27). CONCLUSIONS: Our findings demonstrate that chagasic patients have a higher prevalence of H. pylori compared to non-chagasic subjects; a similar prevalence was found among the diverse clinical forms of the disease. The factors contributing to the frequent co-infection with H. pylori and Trypanosoma cruzi as well as its effects on the clinical outcome deserve further study.

2547 downloads Cited 1 time in SciELO

15.

Influência dos compostos nitrogenados na concentração de proteína da cianobactéria Aphanothece microscopica Nägeli
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Hornes, Marcio

; Silva, Adriana Gonçalves

; Mitterer, Marina Leite

; Queiroz, Maria Isabel

Food Science and Technology

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jun 2010, Volume 30 N. 2 Pages 364 - 371

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

O trabalho teve como objetivo avaliar a influência dos compostos nitrogenados na concentração de proteína da cianobactéria Aphanothece microscopica Nägeli quando cultivada em meio de cultivo padrão BG11 e no efluente do processamento de pescado. As condições experimentais foram inóculo de 200 mg.L-1, 30 °C, pH 7,6 e razão C/N 20. As amostras foram retiradas no início, meio e fim da fase logarítmica e da fase estacionária e analisadas quanto à concentração de nitrogênio total, nitrogênio não proteico, amônio, nitrato, nitrito e ácidos nucleicos. Ficou demonstrado em todas as culturas que o íon amônio, juntamente com os ácidos nucleicos, representa uma importante fração do nitrogênio não proteico presente na biomassa. Os resultados mostraram que o meio de cultivo influencia a concentração de nitrogênio e que a determinação de proteína pelo método de Kjeldahl superestima a concentração proteica em cianobactérias.

This study aimed to evaluate the influence of nitrogen compounds on the protein concentration of the cyanobacterium Aphanothece microscopica Nägeli cultivated in BG11 medium and fish processing effluent. The experimental conditions were 200 mg L-1 inoculum, 30 °C, pH 7.6, and C/N ratio 20. Each culture was harvested during the early, mid-log, and late logarithmic growth phase and during the stationary phase and analyzed in terms of total nitrogen, non-protein nitrogen, ammonium nitrogen, nitrate, nitrite, and nucleic acid. It can be said that in all cultures the ammonium ion and the nucleic acids account for great portion of the non-protein nitrogen in the biomass. The results show that the culture medium influences the nitrogen content, and that the Kjeldahl method overestimates the real protein concentration in cyanobacteria.

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