A full two-level factorial design was employed to study the influence of PEG molar mass (MM PEG), PEG concentration (C PEG) and phosphate concentration (C PHOSPH) on proteases partition by Lentinus citrinus DPUA 1535 in a PEG/phosphate aqueous two-phase system (ATPS). For all ATPS studied, proteases partitioned for the top phase and the best proteases extraction condition was obtained with MM PEG = 6000 g mol-1, C PEG = 17.5% (w/w) and C PHOSPH = 25% (w/w) with (1.1) purification factor and (151%) activity yield. Findings reported here demonstrate a practical strategy that serves as a first step for proteases purification from crude extract by L. citrinus.

Clavulanic acid is a β-lactam antibiotic which has a potent β-lactamase inhibiting activity. In order to optimize its production by the new isolate Streptomyces DAUFPE 3060, the influence of two independent variables, temperature and soybean flour concentration, on clavulanic acid and biomass concentrations was investigated in 250 mL-Erlenmeyers according to a 2² central composite design. To this purpose, temperature and soybean flour (SF) concentration were varied in the ranges 26-34°C and 10-50 g/L, respectively, and the results evaluated utilizing the Response Surface Methodology. The experimental maximum production of clavulanic acid (629 mg/L) was obtained at 32°C and 40 g/L SF after 48 h, while the maximum biomass concentration (3.9 g/L) at 30°C and 50 g/L soybean flour, respectively. These values are satisfactorily close to those (640 mg/L and 3.75 g/L, respectively) predicted by the model, thereby demonstrating the validity of the mathematical approach adopted in this study.

O efeito do polissacarídeo de Anacardium occidentale L. (POLICAJU) foi avaliado na fase inflamatória do processo cicatricial em camundongos (Mus musculus) Swiss (n=90), organizados de acordo com o tratamento empregado: Grupo I (NaCl 150mM), Grupo II (ácido ascórbico 75mg ml-1) e Grupo III (emulsão contendo POLICAJU 150mg ml-1 preparado em ácido ascórbico 75mg ml-1). As lesões cutâneas foram realizadas assepticamente na região torácica dorsal e cada ferida foi tratada em dose única (200µl) imediatamente após a cirurgia. As feridas foram avaliadas diariamente sob o ponto de vista clínico e histopatológico até o 6° dia de pós-operatório (PO). No 5° dia PO, observou-se um menor percentual de edema e hiperemia no Grupo III em relação aos grupos controle, ao passo que os valores de área da ferida e do percentual de contração não foram estatisticamente significativos. A avaliação histopatológica do grupo tratado com POLICAJU demonstrou a presença de tecido de granulação fibrovascular no 6° dia PO, enquanto os grupos controle apresentavam tecido de granulação com padrão vascular. O tratamento proposto propiciou sinais flogísticos menos acentuados (edema e hiperemia) durante o período inflamatório, compatível com o processo de reparação mais avançado do ponto de vista histopatológico, sugerindo a possível utilização clínica da emulsão contendo POLICAJU.

The effect of the polysaccharide from Anacardium occidentale L. gum (POLICAJU) was evaluated in the inflammatory phase of the wound healing in Swiss mice (Mus musculus) (n=90), divided according to the employed treatment: Group I (NaCl 150mM), Group II (75mg ml-1 ascorbic acid) and Group III (emulsion containing 150mg ml-1 POLICAJU prepared in 75mg ml-1 ascorbic acid). An aseptic wound was produced in the dorsal thoracic area and each wound was treated in a single dose (200µl) soon after the accomplishment of the lesions. Daily, the wounds were submitted to the clinical and histopathological evaluation until the 6th day of postoperative (PO). In the 5th day PO, the Group III presented the signs oedema and hyperemia, in smaller percentile than the control groups. The results of area of the wound and percentile of contraction didn’t obtain statistical significance. The histopathological evaluation revealed, in the 6th day PO, that the Group III presented fibrovascular granulation tissue, while the control groups presented vascular granulation tissue. The use of POLICAJU propitiated to the animals of the treated group inflammatory signs less accentuated (oedema and hyperemia) in the inflammatory period, compatible with the normal wound healing process under the point of view histopathological, suggesting the possible clinical employment of the POLICAJU emulsion.