ABSTRACT This research aimed to evaluate the influence of a commercial prebiotic in different concentrations upon several parameters. To carry out the experiment, 640 male one-day-old broiler chicks were distributed in four treatments (0, 0.5, 1.0, and 1.5 kg/ton of yeast cell wall) with eight replicates of 20 birds per experimental unit, in randomized blocks. Prebiotic effects were assessed on performance, carcass yield and prime cuts, in addition to the litter quality (its content of nitrogen and phosphorus). There were significant improvements for weight gain and feed conversion ratio in experimental growth periods. However, prebiotic level at 1.0 kg/ton is enough to provide improvement in performance and similar yield parameters than the control group. Also, 1.5 kg/ton prebiotic inclusion in the diet promotes environmental benefits by reducing the phosphorus amount in the litter by 51%. Above 1.0 kg/ton prebiotic addition in broiler diets can be safely recommended, because it promotes both performance and environmental benefits. experiment 64 onedayold one day old 0, 0 (0 05 5 0.5 10 1 15 1. kgton kg ton wall 2 unit blocks cuts its phosphorus. . phosphorus) periods However group Also 51 51% recommended 6 ( 0.

ABSTRACT The objective of this study was to evaluate whether the addition of different levels of capsaicin in the diet of female turkeys has beneficial effects on growth performance and the antioxidant and oxidant status in serum and meat. A total of 150 female turkeys were distributed in a completely randomized design with three treatments with five replicates per treatment and ten birds per experimental unit. Treatments were identified as control (without additive); T400 – basal diet containing 400 mg/kg of pepper extract per kg of feed; and T800 – basal diet supplemented with 800 mg/kg of pepper extract per kg of feed. Growth performance was measured on days 1 and 20 of the experiment. Blood samples were collected at 20 days of the experiment for analysis of the oxidant and antioxidant status, and at 20 days, five birds were euthanized per treatment for the analysis of the oxidant and antioxidant status of the meat. The turkeys fed diet supplemented with capsaicin had lower feed intake and better feed:gain ratio. Turkeys in the T800 treatment showed a reduction in serum levels of reactive oxygen species (ROS) and thiobarbituric acid, and this same effect was observed for ROS in their breast meat. Capsaicin supplementation improves feed conversion and reduces feed intake without altering weight gain in female turkeys. Furthermore, the addition of 800 g/ton reduces lipid peroxidation and protein oxidation in the serum and reduces protein oxidation in broiler turkey meat. meat 15 unit additive additive) T T40 40 mgkg mg T80 80 2 feedgain ratio (ROS acid Furthermore gton g ton T4 4 T8 8

ABSTRACT In this study, we evaluated how guanidinoacetic acid (GAA) addition in diets with various metabolizable energy (ME) contents affects the performance of broiler chickens. We also estimated the equivalence of GAA in ME. We distributed 1,280 one-day-old broilers in a completely randomized design with eight treatments, eight replicates, and twenty birds per experimental unit. Treatments were based on ME levels (2,775-2,875-2,975 kcal/kg; 2,850-2,950-3,050 kcal/kg; 2,925-3,025-3,125 kcal/kg; or 3,000-3,100-3,200 kcal/kg, from 1 to 7, 8 to 21, and 22 to 42 days of age) and the inclusion of GAA (0 or 600 mg/kg). Supplementation of GAA increased weight gain in broilers at an energy level of 2,908 kcal/kg and improved feed conversion ratio (FCR) at energy levels of 2,908 and 2,983 kcal/kg. There was a linear reduction in feed intake and an improvement in FCR of broilers with increasing levels of energy in diets, with and without GAA addition. Solving the equivalence equation, by applying each of the weighted average energy levels studied. indicates the GAA equivalence of 133, 103, 74, and 44 kcal/kg of diet. In conclusion, GAA supplementation improves broilers’ efficiency of energy use; the average ME equivalence of 600 mg/kg of GAA is 88.5 kcal/kg. study (GAA (ME chickens 1280 280 1,28 onedayold one day old treatments replicates unit 2,7752,8752,975 277528752975 2,775 2,875 2,975 2 775 875 975 (2,775-2,875-2,97 kcalkg kcal kg 2,8502,9503,050 285029503050 2,850 2,950 3,050 850 950 3 050 2,850-2,950-3,05 2,9253,0253,125 292530253125 2,925 3,025 3,125 925 025 125 2,925-3,025-3,12 3,0003,1003,200 300031003200 3,000 3,100 3,200 000 100 200 3,000-3,100-3,20 7 21 4 age 0 ( 60 mg/kg. mgkg . mg mg/kg) 2908 908 2,90 (FCR 2983 983 2,98 equation studied 133 103 74 diet conclusion use 885 88 5 88. 128 28 1,2 7752 8752 2,7752,8752,97 27752875297 2775 2,77 2875 2,87 2975 2,97 77 87 97 (2,775-2,875-2,9 8502 9503 2,8502,9503,05 28502950305 2850 2,85 2950 2,95 3050 3,05 85 95 05 2,850-2,950-3,0 9253 0253 2,9253,0253,12 29253025312 2925 2,92 3025 3,02 3125 3,12 92 02 12 2,925-3,025-3,1 0003 1003 3,0003,1003,20 30003100320 3000 3,00 3100 3,10 3200 3,20 00 10 20 3,000-3,100-3,2 6 290 90 2,9 298 98 13 1, 2,7752,8752,9 2775287529 277 2,7 287 2,8 297 9 (2,775-2,875-2, 2,8502,9503,0 2850295030 285 295 305 3,0 2,850-2,950-3, 2,9253,0253,1 2925302531 292 302 312 3,1 2,925-3,025-3, 3,0003,1003,2 3000310032 300 310 320 3,2 3,000-3,100-3, 29 2, 2,7752,8752, 277528752 27 (2,775-2,875-2 2,8502,9503, 285029503 30 3, 2,850-2,950-3 2,9253,0253, 292530253 31 2,925-3,025-3 3,0003,1003, 300031003 32 3,000-3,100-3 2,7752,8752 27752875 (2,775-2,875- 2,8502,9503 28502950 2,850-2,950- 2,9253,0253 29253025 2,925-3,025- 3,0003,1003 30003100 3,000-3,100- 2,7752,875 2775287 (2,775-2,875 2,8502,950 2850295 2,850-2,950 2,9253,025 2925302 2,925-3,025 3,0003,100 3000310 3,000-3,100 2,7752,87 277528 (2,775-2,87 2,8502,95 285029 2,850-2,95 2,9253,02 292530 2,925-3,02 3,0003,10 300031 3,000-3,10 2,7752,8 27752 (2,775-2,8 2,8502,9 28502 2,850-2,9 2,9253,0 29253 2,925-3,0 3,0003,1 30003 3,000-3,1 2,7752, (2,775-2, 2,8502, 2,850-2, 2,9253, 2,925-3, 3,0003, 3,000-3, 2,7752 (2,775-2 2,8502 2,850-2 2,9253 2,925-3 3,0003 3,000-3 (2,775- 2,850- 2,925- 3,000- (2,775 (2,77 (2,7 (2, (2

ABSTRACT The objective of this study was to evaluate the effects of different levels of microencapsulated carvacrol and cinnamaldehyde on metabolizable energy, feed digestibility, intestinal morphometric analysis, and antioxidant parameters in broilers. A completely randomized design with five treatments and eight replications of eight broilers (Cobb male) was used, and collections were carried out at 20 and 42 days of age. Carvacrol and cinnamaldehyde concentrations (mg/kg) were: 0 (control), 50, 100, 150, and 200. Carvacrol and cinnamaldehyde improved apparent metabolizable energy but did not change protein and ether extract digestibility. Supplementation increased intestinal villus height and villi:crypt ratio; in which 100 mg/kg produced the greatest villus height. Serum uric acid levels were lower in birds receiving supplementation. Improvement in the activity of glutathione peroxidase and glutathione-S-transferase was observed, while lower uric acid, thiobarbituric acid reactive substances, and reactive oxygen species levels were observed. Microencapsulated carvacrol and cinnamaldehyde improve apparent metabolizable energy and can be administered in broiler feed without risk to the bird’s health. These supplements may serve as alternative products to aid the performance of commercial poultry. digestibility analysis Cobb male used 2 4 age mgkg mg kg (mg/kg control, control , (control) 50 150 200 villicrypt villi crypt ratio 10 supplementation glutathioneStransferase S transferase observed substances bird s health poultry (control 5 15 1

ABSTRACT The objective of this study was to evaluate whether the addition of the enzymes phytase and xylanase, isolated or associated, in the liquid form after feed pelletization could improve energy utilization and digestibility of calcium and phosphorus by broiler chickens. Three experiments were performed using 120 birds each, divided into five treatments with eight replicates per group (n = 3), identified as: experiment 1 (xylanase: control, 1000 IU, 1500 IU, 2000 IU, 2500 IU), experiment 2 (phytase: control, 500 FTU, 1000 FTU, 1500 FTU, 2000 FTU), experiment 3 (xylanase + phytase: control, 3000 IU + 500 FTU, 3000 IU + 1000 FTU, 3000 IU + 1500 FTU, 3000 IU + 2000 FTU). Samples for digestibility tests were collected at 14 to 21 days of age. Therefore, the inclusion of liquid phytase and liquid phytase + xylanase after pelletization in broiler diets has become a relevant way to reduce the inclusion of inorganic P, which can reduce the cost of feed and P excretion in the environment. Furthermore, it is an interesting strategy to avoid enzyme denaturation in the pelleting process. associated chickens 12 each n 3, , 3) as control 100 150 200 250 IU) (phytase 50 FTU FTU) 300 FTU. . age Therefore environment Furthermore process 10 15 20 25 5 30

RESUMO As dietas de frangos de corte são baseadas na produção de milho, soja e trigo; no entanto, alguns ingredientes proteicos possuem muitos fatores antinutricionais e baixa digestibilidade. O objetivo deste estudo foi adicionar à ração uma mistura de enzimas exógenas com baixo valor nutricional para frangos de corte para reduzir os custos de produção e melhorar a digestibilidade, mantendo o bom desempenho zootécnico. O delineamento experimental foi inteiramente casualizado, incluindo três tratamentos com cinco repetições (n=15) cada: a) controle positivo (PC), dieta calculada para machos com desempenho médio; b) controle negativo + enzimas exógenas (CN+EE), dieta mínima de nutrientes de acordo com as exigências da fase de produção, e foi adicionada a mistura enzimática; c) controle negativo (CN), teores mínimos de nutrientes para cada fase. Aos 21 dias, o grupo PC apresentou maior ganho de peso e menor conversão alimentar que o NC (P<0,05). Aos 42 dias, PC apresentou menor consumo de ração que NC (P=0,040), enquanto foi observada menor conversão alimentar nos grupos PC e NC+EE que NC (P=0,001). O índice de eficiência de produção foi maior no tratamento PC, mas o tratamento NC+EE foi maior que o NC (P=0,001). Considerando os custos de produção e peso corporal, verificamos que as aves NC+EE tiveram maior rentabilidade. Portanto, concluímos que a mistura de enzimas exógenas adicionada a uma dieta com níveis nutricionais mínimos tem aplicação prática no sistema de produção de frangos de corte.

ABSTRACT Broiler diets are based on corn, soybean and wheat production; however, some protein ingredients have many antinutritional factors and low digestibility. The objective of this study was to add a blend of exogenous enzymes to the feed with low nutritional value for broilers to reduce production costs and improve digestibility while maintaining good zootechnical performance. The experimental design was completely randomized, including three treatments with five replications (n=15) each: a) positive control (PC), diet calculated for males with average performance; b) negative control + exogenous enzymes (NC+EE), minimum nutrient diet according to the requirements of the production phase, and the enzymatic blend was added; and c) negative control (NC), minimum levels of nutrients for each phase. At 21 days, the PC group showed greater weight gain and lower feed conversion than the NC (P<0.05). At 42 days, PC had lower feed intake than NC (P=0.040), while lower feed conversion was observed in groups PC and NC+EE than NC (P=0.001). The production efficiency index was higher in the PC treatment, but the NC+EE treatment was higher than the NC (P=0.001). Considering production costs and body weight, we found that NC+EE birds had greater profitability. Therefore, we conclude that the blend of exogenous enzymes added to a diet with minimal nutritional levels has practical application in the broiler production system.

ABSTRACT. The objective of this study was to determine choline chloride replacement effects by a vegetable choline source, compost by Trachyspermum amni, Citrullus colocynthis, Achyranthus aspera, and Azadirachta indica in broiler feed. These compounds are fonts of phosphatidylcholine, a high-disponible molecule for intestinal absorption and choline supply. A total of 640 animals were randomly allocated in a completely randomized design, with four treatments and eight repetitions (n = 20), and zootechnical performance (body weight, weight gain, feed conversion ratio, and productive efficiency index), carcass yield, cuts yield, and organs (heart, liver, proventriculus, gizzard, and small intestine) relative weights were evaluated. Were evaluated two choline chloride levels (600 and 800 mg kg-1) and two vegetable choline levels (100 and 200 mg kg-1), added in a corn-soybean meal basal diet, during 42 days of raising. Results revealed better feed conversion ratio (p < 0.001) and production efficiency index (p < 0.001) in broilers fed vegetable choline, with no differences on body weight (p = 0.372) and weight gain (p = 0.427) among broilers. Carcass, cuts yield, and organ relative weights do not alter (p > 0.05) due to different group of supplementations. Findings in this trial concludes vegetable choline can adequately replace choline chloride in broiler feed, with improvement on performance and no compromising carcass, cuts or organ development.

ABSTRACT The objective of this study was to evaluate performance, hematological parameters, leukocyte counts, and respiratory microbiota of broilers under heat stress receiving eucalyptus oil supplementation. Cobb broilers (n = 1200) were randomly distributed on the first day of life in a factorial arrangement with additional treatment (2 × 2 + 1) with two periods of oil administration via drinking water (daily, from 18 to 35 days of life) and two periods of oil administration via spray (daily, from 18 to 35 days of age) plus a control treatment, consisting of twelve replications, with 20 animals each. The birds were kept in a controlled environment with constant temperature at 32 °C to induce heat stress by infrared heaters. Eucalyptus oil was supplied by drinking water (1 L of oil to 4000 L of water) or by spray applications with a pump (1 L of oil to 200 L of water). Bird weight was recorded at 42 days of age, along with remaining feed, to determine weight gain, feed intake, and feed conversion. In addition, one blood sample from each bird repetition was collected for hematological and leucocytic evaluation. To assess respiratory microbiota, a tracheal flushing was performed for bacteria counts. There was no difference in performance, hematological parameters, and leukocyte counts, except hematocrit, which was lower in birds that received eucalyptus oil after 18 days of age. No significant differences were observed in the respiratory microbiota comparing oil-treated and -untreated groups. Although it was not possible to verify statistical difference, the birds that received eucalyptus oil via spray plus spray from 18 days old showed lower bacterial counts and absence of isolation of Gram negatives, while the control group was the one with the highest number of Gram negatives. Therefore, eucalyptus oil can be used for heat-stressed broilers without impairing their development.

RESUMO As aves possuem baixo aproveitamento do fósforo fítico das dietas, devido à falta de enzimas endógenas que consigam realizar a quebra e aproveitamento do mesmo. Objetivou-se no presente estudo avaliar a adição de fitase em dietas de codornas poedeiras. Foram utilizadas 80codornas, em delineamento experimental inteiramente casualizado composto por cinco tratamentos e quatro repetições, de quatro aves cada. Os tratamentos foram: 0,35% Pd; 0,20% Pd; 0,20% Pd + 500FTUs de fitase; 0,09% Pd; 0,09% Pd + 500 FTUs de fitase. As aves foram alojadas em baterias metálicas, em período experimental de 21 dias, para avaliação da produção de ovos, peso dos ovos, consumo de ração e conversão alimentar. Após coletados, os ovos foram encaminhados ao laboratório de nutrição do departamento de zootecnia do CEO/Udesc, onde foram realizadas as análises de qualidade de albúmen, de clara e casca. Os resultados obtidos foramsubmetidos à análise de variância e as diferenças entre as médias foram comparadas pelo teste de Tukey a 5% de significância. Não foram observadas diferenças (P>0,05) nos parâmetros de desempenho e qualidade de ovos avaliados. Conclui-se que a inclusão da fitase na dieta de codornas poedeiras pode ser empregada sem causar comprometimento do desempenho e qualidade dos ovos das aves. Adicionalmente, 0,09% de fósforo disponível pode ser utilizado, garantindo o desempenho em período de 21 dias de produção.

SUMMARY Birds show poor utilization of phytic phosphorus in their diets because of the lack of endogenous enzymes to digest phosphorus and make it bioavailable. Therefore, the objective of this study was to evaluate phytase supplementation in the diets of laying quails. Eighty quails were used in a completely randomized experimental design with five treatments and four replicates of four birds each. The treatments were as follows: 0.35% Pd; 0.20% Pd; 0.20% Pd + 500FTUs of phytase; 0.09% Pd; 0.09% Pd + 500 FTUs of phytase. The birds were housed in metallic batteries during a trial period of 21 days, for evaluation of egg production, egg weight, feed intake and feed conversion. After being collected, the eggs were sent to the nutrition laboratory of the department of zootechnics of the CEO/Udesc, where analyses of albumin, yolk and shell quality were performed. The results were subjected to analysis of variance, and the differences between the averages were compared using the Tukey test at 5% of significance. No differences (P> 0.05) were observed in terms of performance and egg quality parameters. We conclude that the inclusion of phytase in the diet of laying quails can be accomplished without compromising the performance and quality of the eggs. Additionally, 0.09% of 22 disponible phosphorus can be used to grant adequate performance for 21 days.

ABSTRACT Different levels of glycosaminoglycans (chondroitin sulfate and glucosamine sulfate) and vitamin C were injected in ovo to determine whether additive use influences the incubation parameters and bone characteristics of posthatch chicks. Hatchability was not affected by addition of glycosaminoglycans and vitamin C in ovo. However, chicks that received 4 μg additive showed 2.86% reduction in total mortality when compared with chicks from non-injected eggs. Moreover, tibia area and femur bone mineral density increased in chicks from eggs injected with 2.16 and 6.00 μg of additive, respectively. In ovo feeding with glycosaminoglycans and vitamin C can benefit bone development in embryos and reduce total mortality during the incubation period.

The objective of this study was to evaluate the effect of feeding herbal extracts for broilers on performance and histology of the intestinal mucosa and its effects on the profiting from the metabolizable energy of experimental diets. For so, two experiments were conducted. In experiment I, the inclusion of different herbal extracts in diets on performance and intestinal histology of broilers was evaluated, and in experiment II, the values of apparent metabolizable energy and metabolizable energy corrected by the nitrogen balance of the experimental diets were studied. Treatments consisted of: positive control diet; positive control + avilamycin; negative control; negative control + 100 ppm of a complex containing three different herbal medicines (pepper, cinnamon and oregano); negative control + 75 ppm garlic extract; negative control + 150 ppm garlic extract. In the performance experiment, which comprised the period of 1 to 40 days of age, 960 male broilers were distributed in a randomized block design, with six treatments and eight replicates, with 20 birds per experimental unit. In experiment II, the method adopted was the traditional of total excreta collection with male broiler chicks in the age of 14 to 24 days, in a completely randomized design, with six treatments and eight replicates with five birds per experimental unit. The intestinal villus height was improved with addition of the composite containing the three herbal extracts; however, crypt depth and villus/crypt ratio were not affected. The use of herbal extract in diets for broilers promotes performance similar to that with the use of antibiotics. Herbal extracts can be incorporated into diets replacing antibiotics without compromising the metabolizable energy of diets, performance or intestinal mucosa for broilers in the period of 1 to 40 days of age.