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OBJECTIVES: This observational, cross-sectional study based aimed to test whether heart failure (HF)-disease management program (DMP) components are influencing care and clinical decision-making in Brazil. METHODS: The survey respondents were cardiologists recommended by experts in the field and invited to participate in the survey via printed form or email. The survey consisted of 29 questions addressing site demographics, public versus private infrastructure, HF baseline data of patients, clinical management of HF, performance indicators, and perceptions about HF treatment. RESULTS: Data were obtained from 98 centers (58% public and 42% private practice) distributed across Brazil. Public HF-DMPs compared to private HF-DMP were associated with a higher percentage of HF-DMP-dedicated services (79% vs 24%; OR: 12, 95% CI: 94-34), multidisciplinary HF (MHF)-DMP [84% vs 65%; OR: 3; 95% CI: 1-8), HF educational programs (49% vs 18%; OR: 4; 95% CI: 1-2), written instructions before hospital discharge (83% vs 76%; OR: 1; 95% CI: 0-5), rehabilitation (69% vs 39%; OR: 3; 95% CI: 1-9), monitoring (44% vs 29%; OR: 2; 95% CI: 1-5), guideline-directed medical therapy-HF use (94% vs 85%; OR: 3; 95% CI: 0-15), and less B-type natriuretic peptide (BNP) dosage (73% vs 88%; OR: 3; 95% CI: 1-9), and key performance indicators (37% vs 60%; OR: 3; 95% CI: 1-7). In comparison to non- MHF-DMP, MHF-DMP was associated with more educational initiatives (42% vs 6%; OR: 12; 95% CI: 1-97), written instructions (83% vs 68%; OR: 2: 95% CI: 1-7), rehabilitation (69% vs 17%; OR: 11; 95% CI: 3-44), monitoring (47% vs 6%; OR: 14; 95% CI: 2-115), GDMT-HF (92% vs 83%; OR: 3; 95% CI: 0-15). In addition, there were less use of BNP as a biomarker (70% vs 84%; OR: 2; 95% CI: 1-8) and key performance indicators (35% vs 51%; OR: 2; 95% CI: 91,6) in the non-MHF group. Physicians considered changing or introducing new medications mostly when patients were hospitalized or when observing worsening disease and/or symptoms. Adherence to drug treatment and non-drug treatment factors were the greatest medical problems associated with HF treatment. CONCLUSION: HF-DMPs are highly heterogeneous. New strategies for HF care should consider the present study highlights and clinical decision-making processes to improve HF patient care.

https://doi.org/10.6061/clinics/2021/e1991 870 downloads

Fraturas de esterno em uma unidade de tratamento intensivo especializada em trauma
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Pereira, Leonardo Dantas da Silva

; Bassi, Estevão

; Tomazini, Bruno Martins

; Jesus, Vinicius Luiz Menezes

; Tierno, Paulo Fernando Guimarães Morando Marzocchi

; Novo, Fernando Da Costa Ferreira

; Malbouisson, Luiz Marcelo

; Utiyama, Edivaldo Massazo

Revista do Colégio Brasileiro de Cirurgiões

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2019, Volume 46 N. 1 elocation e2059

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO Objetivo: avaliar epidemiologia, características anatômicas, manejo e prognóstico de pacientes críticos com fraturas de esterno. Métodos: análise retrospectiva de pacientes internados em unidade de terapia intensiva (UTI) de emergências cirúrgicas e trauma de um centro de trauma Tipo III em São Paulo, Brasil. Resultados: foram admitidos 1552 pacientes traumatizados no período de janeiro de 2012 a abril de 2016. Desses, 439 apresentavam trauma torácico e 13 apresentavam fratura de esterno, configurando 0,9% das admissões de trauma e 3% dos traumas torácicos. Desses pacientes, três apresentavam tórax instável e dois foram submetidos à conduta cirúrgica para fixação da fratura. A mortalidade de pacientes com fratura de esterno foi de 29% (três pacientes). Em um dos óbitos pôde-se atribuir a fratura do esterno como contribuinte principal para o desfecho. Conclusão: a fratura de esterno foi diagnosticada em 0,9% dos pacientes críticos vítimas de trauma em UTI especializada. Somente 15% dos pacientes necessitaram de conduta cirúrgica específica na fase aguda e a mortalidade foi decorrente das outras lesões na maior parte dos casos.

ABSTRACT Objective: to evaluate epidemiology, anatomical characteristics, management, and prognosis of critical patients with sternum fractures. Methods: retrospective analysis of patients admitted to intensive care unit (ICU) of a Level III trauma center in Sao Paulo, Brazil. Results: 1552 trauma patients were admitted from January 2012 to April 2016. A total of 439 patients had thoracic trauma and among these, 13 patients had sternum fracture, making up 0.9% of all trauma admissions and 3% of all thoracic trauma cases. Three of these 13 patients had unstable chest, two underwent surgical management for fracture fixation, and three died (mortality was of 29%). In one of the deaths, sternum fracture was assessed as the main contributor to the outcome. Conclusion: sternum fracture was diagnosed in 0.9% of critical trauma patients in a specialized ICU. Only 15% of patients required specific surgical management in the acute phase. In most cases, mortality was due to other injuries.

https://doi.org/10.1590/0100-6991e-20192059 6481 downloads

Effect of Etching Temperature on Surface Properties of Ti6Al4V Alloy for Use in Biomedical Applications
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Alberti, Charles Jourdan

; Saito, Eduardo

; Freitas, Filipe Estevão de

; Reis, Danieli Aparecida Pereira

; Machado, João Paulo Barros

; Reis, Adriano Gonçalves dos

Materials Research

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2019, Volume 22 elocation e20180782

Abstract: En | Text: En | PDF: En

The objective of this study is to evaluate the surface physicochemical properties of Ti6Al4V alloy after etching with a piranha’s solution at temperatures of 25 °C, 40 °C, and 60 °C for 30 min. Scanning electron microscopy with energy-dispersive X-ray spectroscopy, optical profilometry, X-ray diffraction, and wettability analyses were used to characterize the surface of the samples. In addition, corrosion tests were performed using potentiodynamic polarization and electrochemical impedance spectroscopy. The proposed surface treatment did not alter the wettability and surface chemistry (crystallinity), but the texture, surface roughness, superficial area, and corrosion resistance of the alloy could be changed by varying the etching temperature. Considering that these properties are indirect indicators of the alloy’s potential success in the osseointegration of a dental implant, the surface treatment of the Ti6Al4V alloy using a piranha solution while controlling the etching temperature has attractive biomedical applications.

https://doi.org/10.1590/1980-5373-mr-2018-0782 789 downloads

Essential oils from Hyptis marrubioides, Aloysia gratissima and Cordia verbenacea reduce the progress of Asian soybean rust
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Silva, André Costa da

; Souza, Paulo Estevão de

; Amaral, Douglas Carvalho

; Zeviani, Walmes Marques

; Pinto, José Eduardo Brasil Pereira

Acta Scientiarum. Agronomy

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jun 2014, Volume 36 N. 2 Pages 159 - 166

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

O objetivo do trabalho foi avaliar o potencial dos óleos essenciais extraídos de Hyptis marrubioides, Aloysia gratissima e Cordia verbenacea no controle da ferrugem asiática da soja. A atividade inibitória dos óleos essenciais (aplicados no intervalo de concentração de 0,05 - 2%) sobre a germinação dos urediniósporos de Phakopsora pachyrhizi foram avaliados em teste in vitro. As propriedades curativas e erradicantes dos óleos (aplicados no intervalo de concentração de 0,05 - 0,5%) foram estudadas em casa de vegetação utilizando a cultivar MG/BR 46 (Conquista) suscetível a P. pachyrhizi. Microscopia eletrônica de varredura foi utilizado para investigar o efeito dos óleos essenciais sobre a morfologia dos urediniósporos do fungo. Os tratamentos com os óleos essenciais, em todas as concentrações testadas inibiram totalmente a germinação dos urediniósporos in vitro. A aplicação curativa dos óleos essenciais reduziu a severidade da doença, expressa em área abaixo da curva de progresso em 33 a 41%, enquanto que o fungicida comercial (piraclostrobina + epoxiconazole), empregado como controle positivo reduziu em 61%. Tratamento de plantas infectadas com óleos essenciais produziu alterações morfológicas nas estruturas fúngicas, semelhantes as produzidas pelo fungicida comercial, ou seja, murchamento dos urediniósporos, apressórios, tubos germinativos e paráfise.

The aim of this study was to evaluate the potential of essential oils derived from Hyptis marrubioides, Aloysia gratissima and Cordia verbenacea for controlling Asian soybean rust. The inhibitory activities of the essential oils (applied in a concentration range of 0.05 - 2%) on the germination of Phakopsora pachyrhizi urediniospores were investigated using in vitro assays. The curative and eradicating properties of the oils (applied in a concentration range of 0.05 - 0.5%) were studied under greenhouse conditions using the P. pachyrhizisusceptible soybean cultivar MG/BR 46 (Conquista). Scanning electron microscopy was employed to investigate the effects of the essential oils on the morphology of the P. pachyrhizi urediniospores. The treatment with the essential oils at all concentrations tested led to the total inhibition of spore germination in vitro. The curative application of the essential oils reduced the disease severity, expressed as the area under the disease-progress curves, by 33 to 41%, whereas the commercial fungicide (pyraclostrobin + epoxyconazole) employed as a positive control reduced the severity by 61%. The treatment of infected plants with the essential oils resulted in morphological alterations in the fungal structures that were similar to those produced by the commercial fungicide, namely, a shrivelling of the urediniospores, appressoria, germ tubes and paraphyses.

https://doi.org/10.4025/actasciagron.v36i2.17441 1940 downloads

Olhos de ver, ouvidos de ouvir, mãos de fazer: oficinas de atividades em Terapia Ocupacional como método de coleta de dados
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Pereira, Paulo Estevão

; Malfitano, Ana Paula Serrata

Interface - Comunicação, Saúde, Educação

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jun 2014, Volume 18 N. 49 Pages 415 - 422

Abstract: Pt En Es | Text: Pt En Es | PDF: Pt | PDF: En | PDF: Es

Os métodos de coleta de dados em pesquisa podem ser múltiplos e envolvem diferentes técnicas e abordagens. Partindo da perspectiva teórico-metodológica da Terapia Ocupacional Social, apresenta-se o relato de fragmentos de oficinas de atividades utilizadas como recurso para apreensão da realidade de jovens pobres residentes em periferias urbanas, no desenvolvimento de uma determinada pesquisa. A partir de imagens e poesias produzidas pelos jovens colaboradores, em atividades realizadas em um centro de juventude, conclui-se que as oficinas terapêutico-ocupacionais possibilitaram não “apenas” a produção de ações com aqueles jovens, mas também apresentaram-se como um importante e potente recurso para a apreensão de suas realidades no âmbito da pesquisa.

Data-gathering methods in research may be multiple and involve many techniques and approaches. Starting from the theoretical and methodological perspective of social occupational therapy, a report on fragments of activity workshops that were used as a resource for understanding the realities of adolescents living in peripheral urban areas, in developing a particular research project is presented. Through images and poetry produced by adolescents participating in activities at a youth center, it is concluded that occupational therapeutic workshops not only enable production of actions among these adolescents, but also are an important and potent resource for comprehending their realities within the scope of research.

Los métodos de colecta de datos en las investigaciones pueden ser múltiplos y envuelven diferentes técnicas y abordajes. Partiendo de la perspectiva teórico-metodológica de la Terapia Ocupacional Social se presenta el relato de fragmentos de talleres de actividades utilizados como recurso para la percepción de la realidad de jóvenes pobres residentes en periferias urbanas, en el desarrollo de un estudio determinado. A partir de imágenes y poesías producidas por los jóvenes colaboradores, en actividades realizadas en un centro para la juventud, se concluye que los talleres terapéutico-ocupacionales posibilitaron no “solo” la producción de acciones con esos jóvenes, sino que también se presentaron como un importante y potente recurso para la percepción de sus realidades en el ámbito de la investigación.

https://doi.org/10.1590/1807-57622013.0784 2536 downloads

Olhos de ver, ouvidos de ouvir, mãos de fazer: oficinas de atividades em Terapia Ocupacional como método de coleta de dados
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Pereira, Paulo Estevão

; Malfitano, Ana Paula Serrata

Interface - Comunicação, Saúde, Educação

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apr 2014, Volume 18 N. 49 Pages 415 - 422

Abstract: Pt En Es | Text: Pt En Es | PDF: Pt | PDF: En | PDF: Es

https://doi.org/10.1590/1807-57622013.0784 789 downloads Cited 1 time in SciELO

Progresso da seca da haste (Botrytis cinerea) do hibisco (Hibiscus sabdariffa) em quatro épocas e dois métodos de plantio
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Oliveira, Carlos Alberto de

; Souza, Paulo Estevão de

; Pozza, Edson Ampélio

; Pinto, José Eduardo Brasil Pereira

; Barretti, Patrícia Baston

Summa Phytopathologica

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jun 2013, Volume 39 N. 2 Pages 110 - 116

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

O progresso da seca da haste em hibisco foi estudado em quatro épocas e dois métodos de plantio. O delineamento experimental foi em blocos ao acaso, com quatro repetições e os oito tratamentos foram dispostos em esquema fatorial 4 (épocas de plantio) x 2 (métodos de plantio), sendo: semeadura direta em 05/02/03, 06/03/03, 05/04/03 e 15/05/03; transplantio de mudas em 24/12/02, 25/01/03, 24/02/03 e 25/03/03. Imediatamente após o surgimento dos sintomas, avaliou-se a doença a cada 10 dias até o final do ciclo, aos 205 dias, calculando-se a porcentagem de hastes infectadas por planta. Calculou-se a área abaixo da curva de progresso para a incidência (AACPI). As curvas de progresso da doença dos tratamentos foram submetidas ao ajuste dos modelos linear, exponencial, monomolecular, logístico e Gompertz. Houve interação significativa de métodos e épocas de plantio na incidência da doença. Constatou-se menor incidência da seca da haste em transplantio de mudas comparado à semeadura direta em todas as épocas de plantio. Verificou-se aumento da incidência proporcionado pelo atraso na época de plantio nos dois métodos. O modelo exponencial foi o que melhor descreveu o comportamento da doença em todos os tratamentos. As diferenças estatísticas entre os tratamentos, considerando a taxa de progresso, não refletiram a intensidade da doença no campo. Na mesma época de plantio, as quantidades inicial e máxima da doença, observadas na semeadura direta, foram superiores aos tratamentos referentes ao transplantio de mudas, coerentes com os valores da AACPI. Registrou-se uma relação direta entre queda de temperatura e aumento da incidência da seca da haste.

Stem blight progress in roselle was studied in four periods and two planting methods. Experimental design was in randomized blocks with four replicates and eight treatments in 4 (periods) x 2 (planting methods) factorial arrangement: direct sowing on 05/02/03, 06/03/03, 05/04/03, 15/05/03 and seedling transplanting on 24/12/02, 25/01/03, 24/02/03, 25/03/03. Immediately after the emergence of symptoms, the disease was evaluated at every 10 days until the end of the cycle, at 205 days, and the percentage of infected stems was calculated. The area under the progress curve of the incidence (AUPCI) was calculated. The disease progress curves for the treatments were adjusted to linear, exponential, monomolecular, logistic and gompertz models. There was significant interaction of methods and planting periods for the disease incidence. Lower incidence of stem blight was noted for seedling transplanting compared to direct sowing in all planting periods. There was increased incidence due to the delay in planting for the two methods. The exponential model best described the disease behavior in all treatments. The statistical differences among treatments considering the progress rate did not reflect the disease intensity in the field. In the same planting period, the initial and maximum quantities of the disease, at the direct sowing, were superior to the treatments related to the seedling transplanting, consistent with the AUPCI values. A direct relationship was recorded between temperature fall and increased incidence of stem blight.

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Effectiveness of essential oils in the treatment of Colletotrichum truncatum-infected soybean seeds
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Silva, André Costa da

; Souza, Paulo Estevão de

; Machado, José da Cruz

; Silva, Bruno Marques da

; Pinto, José Eduardo Brasil Pereira

Tropical Plant Pathology

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oct 2012, Volume 37 N. 5 Pages 305 - 313

Abstract: En | Text: En | PDF: En

The aim of the study was to evaluate the antifungal activity of essential oils from "hortelã do campo" (Hyptis marrubioides), "alfazema-do-Brasil" (Aloysia gratissima) and "erva-baleeira" (Cordia verbenacea) and their efficacy in the treatment of soybean seeds infected with Colletotrichum truncatum. In vitro assays were performed to evaluate the effects of the oils on spore germination, mycelial growth, and the production and viability of C. truncatum conidia. Soybean seeds inoculated with C. truncatum were treated with essential oils at concentrations ranging from 0.5 to 2% and grown under greenhouse conditions. Seed health and germination percentages were evaluated according to standard protocols. Seedling emergence, stand establishment and the percentages of dead seedlings were determined, together with the emergence speed index, plantlet height and dry weight of aerial biomass. The oils from H. marrubioides, C. verbenacea and A. gratissima inhibited the germination and production of conidia as well as the growth of C. truncatum. At concentrations ≥ 1%, the effectiveness of the oils against soybean anthracnose was superior to, or at least comparable with, that of the fungicide carbendazim. The viability of conidia and the germination of soybean seeds were not affected by any of the treatments with essential oils. It is concluded that essential oils from H. marrubioides, A. gratissima and C. verbenacea have potential as alternatives to synthetic fungicides in the control of anthracnose in soybean seeds.

4718 downloads Cited 2 times in SciELO

Efeito de variáveis ambientais, épocas e métodos de plantio na intensidade da seca da haste (Botrytis cinerea) em Hibiscus sabdariffa
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Oliveira, Carlos Alberto de

; Souza, Paulo Estevão de

; Pozza, Edson Ampélio

; Pinto, José Eduardo Brasil Pereira

; Barretti, Patrícia Baston

Summa Phytopathologica

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jun 2011, Volume 37 N. 2 Pages 101 - 106

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

O presente estudo objetivou avaliar o efeito da temperatura (15, 20, 25 e 30ºC), do período de molhamento foliar (0, 6, 12 e 24 h), de épocas (setembro, outubro, novembro e dezembro) e métodos de plantio (semeadura direta e transplantio de mudas), na intensidade da seca da haste (Botrytis cinerea) do hibisco (Hibiscus sabdariffa). As variáveis ambientais foram avaliadas em condições controladas com inoculação artificial e as épocas e métodos de plantio foram avaliados em condições de infecção natural em campo. Os dados de frequência de infecção analisados, como área abaixo da curva de progresso da frequência de infecção (AACPF) e comprimento de lesões relacionados às variáveis ambientais, foram submetidos à análise de variância e regressão e, em seguida, plotadas as superfícies de resposta. Os dados de incidência (AACPI) relacionados às épocas e métodos de plantio foram submetidos à análise de variância, utilizando-se o programa estatístico Sisvarâ/UFLA. A interação da temperatura e da duração do período de molhamento foliar influenciou a frequência de infecção e o comprimento de lesões da seca da haste. Houve aumento na frequência de infecção e no comprimento de lesões com o incremento do período de molhamento foliar e redução da temperatura. As lesões apresentaram maior tamanho na temperatura de 15ºC e 24 horas de molhamento foliar. Na ausência de molhamento foliar houve manifestação de sintomas somente a 15ºC. A 30ºC houve dependência de maior período de molhamento foliar para a manifestação de sintomas. Houve interação significativa de métodos e épocas de plantio na incidência da doença. Constatou-se menor incidência da seca da haste em transplantio de mudas comparado à semeadura direta em todas as épocas de plantio. Verificou-se aumento da incidência proporcionado pelo atraso na época de plantio nos dois métodos. Registrou-se uma relação direta entre queda de temperatura e aumento da incidência da seca da haste.

The aim of the present study was to evaluate the effect of temperature (15, 20, 25 and 30ºC), leaf wetness period (0, 6, 12 and 24 h), and planting time (September, October, November and December) and method (direct sowing and transplanting) on the intensity of stem blight (Botrytis cinerea) in Hibiscus sabdariffa. Environmental variables were evaluated under controlled conditions with artificial inoculation, while planting times and methods were assessed under natural infection conditions in the field with natural inoculation. Infection frequency data, analyzed as area under the progress curve of infection frequency (AUPCF), and lesion length related to the environmental variables were subjected to analysis of variance and regression; then, response surfaces were plotted. Incidence data (AUPCI) related to planting times and methods were subjected to analysis of variance using the statistical program Sisvarâ/UFLA. The interaction of temperature and leaf wetness period influenced the infection frequency and the length of stem blight lesions, which increased with the increase in leaf wetness period and with the decrease in temperature. Lesions were larger at 15ºC and 24 hours of leaf wetness. In the absence of leaf wetness, symptoms manifested only at 15ºC. At 30ºC, longer leaf wetness periods were required for symptoms to manifest. There was a significant interaction of planting times and methods on the disease incidence. Stem blight incidence was lower in seedling transplanting compared to direct sowing at all planting times. There was an increase in incidence due to the delay in planting time with both methods. Thus, there was a direct relationship between decrease in temperature and increase in stem blight incidence.

2565 downloads Cited 2 times in SciELO

10.

Fungitoxic activity avaliation of the hexane and methanol extracts of copaiba plant leavesCopaifera langsdorffi Desfon
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Amorim, Ana Carolina Lourenço

; Cardoso, Maria das Graças

; Pinto, José Eduardo Brasil Pereira

; Souza, Paulo Estevão de

; Delú Filho, Nelson

Ciência e Agrotecnologia

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apr 2004, Volume 28 N. 2 Pages 314 - 322

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Avaliou-se a fungitoxicidade dos extratos hexânico e metanólico do óleo copaíba por meio de ensaios biológicos contra os fitopatógenos Colletotrichum gloeosporioides e Bipolaris sorokiniana. Os referidos extratos foram escolhidos em razão dos melhores rendimentos obtidos na extração a frio. O material biológico foi obtido na UFLA no Departamento de Fitopatologia, repicado em meio de cultura BDA (C. gloeosporioides) e PCA (B. sorokiniana) e incubados em câmara de germinação com controle de temperatura e luz. Empregaram-se cinco tratamentos (0/100/200/400/800 ppm), realizando-se três repetições e avaliando-se quatro raios, medidos em centímetros, a partir do micélio central. Os valores aferidos foram aplicados à fórmula do Índice de Crescimento Micelial (ICM) para as análises estatísticas. Observou-se que o extrato metanólico apresentou uma tendência à redução de 32% contra o B. sorokiniana, ao passo que o hexânico foi eficaz para ambos os fitopatógenos, apresentado uma tendência à redução de 49% para o B. sorokiniana e 18% para o C. gloeosporioides.

The fungitoxicity of hexane and methanol extracts of copaiba plants were evaluated by biological tests against the phytopathogens Colletotrichum gloeosporioides and Bipolaris sorokiniana. These extracts were chosen because they presented the highest yields in the extraction at room temperature. The biological material was obtained from the Department of Phytopathology of the UFLA, transplanted in BDA (C. gloeosporioides) and PCA (B. sorokiniana) culture media and incubated in a germination chamber with control of light and temperature. Five treatments (0/100/200/400/800 ppm) were used with three repetitions of each, and four mutually perpendicular radii, were measured in centimeters from the mycelial center. The formula for the Mycelial Growth Index (MGI) was applied to the values obtained for statistical analyses. A 32% reduction in the growth of B. sorokiniana was observed in the presence of the methanol extract, while the hexane extract was effective against both of the phytopathogens, resulting in a 49% reduction in the growth of B. sorokiniana and an 18% reduction in the growth of C. gloeosporioides.

1669 downloads Cited 1 time in SciELO

11.

Doenças foliares da alfafa (Medicago sativa L.), em Lavras, Minas Gerais
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Pereira, Rosana Cristina

; Evangelista, Antônio Ricardo

; Souza, Paulo Estevão de

; Silva, Alexandre Christófaro

; Muniz, Joel Augusto

Revista Brasileira de Zootecnia

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dec 2003, Volume 32 N. 6 Pages 1287 - 1296

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Objetivou-se verificar as doenças foliares que ocorrem na alfafa em Lavras-MG, no período de setembro de 1997 a outubro de 1998. Um ensaio foi conduzido em blocos ao acaso em esquema de parcelas subdivididas. Nas parcelas foram alocadas foram 35 cultivares e nas subparcelas 11 épocas de avaliação. Avaliações foram realizadas aos 28 dias após corte, quando foram coletadas dez hastes de alfafa por parcela para avaliação da desfolha e determinação da severidade das doenças. Os dados foram submetidos à análise de variância e os contrastes entre médias comparados pelo teste de Scott-Knott a 5%. A mancha-de-Leptosphaerulina ocorreu durante o ano todo, porém com maior severidade nos períodos mais frios do ano. A mancha-negra-das-folhas-e-caule apresentou maior severidade no final do verão e início do outono e a ferrugem revelou maior severidade apenas nas avaliações de novembro. As maiores porcentagens de desfolha foram obtidas nos meses mais frios do ano. As cultivares mais resistentes à mancha-de-Leptosphaerulina, cercosporiose e à desfolha foram a Crioula, P30 e Costera SP INTA. As cultivares MH 15 e MH 4 destacaram-se pela maior resistência à ferrugem.

It was aimed to verify the chief leaf diseases that occurs in alfalfa, in Lavras-MG, state of Minas Gerais, of 1997 september to 1998 october. A randomized block trial subplots in time, whose factors were 35 alfalfa cultivars in the plots and 11 evaluation times, which corresponded to the months, in subplots. Evaluations were performed at 28 days after the cut, when ten stems here collected by plots for evaluation of defoliation and determination of severity of the diseases. The data here submitted to the variance analysis and the contrasts among averages compared by Scott-Knott test at 5%. The leaf spot happened during the whole year, with a greatest severity in the periods more colds of the year. The summer black stem and leaf spot, it presented greater severity at the end of summer and beginning of the autumn and the rust reveled a greatest severity in the evaluations of November. The most resistant cultivars to leaf spot, summer black stem and leaf spot and defoliation were Crioula, P 30 and Costera SP INTA. The cultivars MH 15 and MH 4 stood out by larger resistance to the rust.

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12.

Pesquisa de anticorpos contra o vírus da Estomatite Vesicular em bovinos de corte criados na região de Araçatuba, Estado de São Paulo, Brasil em 2000
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De Stefano, Eliana

; Araújo, Wanderley Pereira de

; Passos, Estevão de Camargo

; Pituco, Edviges Maristela

Brazilian Journal of Veterinary Research and Animal Science

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2003, Volume 40 N. 1 Pages 29 - 35

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

No ano de 2000 foram pesquisadas 1099 amostras de soro de bovinos de corte, adultos, pertencentes à seis rebanhos situados nos municípios de Auriflama, Gastão Vidigal,, Magda, Santo Antônio do Aracanguá, Sebastianópolis do Sul e Turiúba, região de Araçatuba. A técnica empregada para a dosagem de anticorpos para o vírus da Estomatite Vesicular foi a soroneutralização em microplacas, utilizando a cepa Indiana 1 Costa Rica/72 com título de 10(6,0) DICT50/50mL. As células utilizadas foram as da linhagem de Rim de Macaco Verde Africano (VERO-CCL 81), provenientes do American Type Cell Colection (ATCC). O título de anticorpos de cada amostra de soro, foi calculado como a recíproca da maior diluição do soro expresso em log10, que protegeu 100,00% da monocamada celular. Foram consideradas positivas as amostras com título superior a 1,6 log10 (1/40). Das 1099 amostras de soro analisadas, 28 (2,50%) foram positivas ao vírus da Estomatite Vesicular tipo Indiana 1, com títulos de anticorpos variando de 1,9 à 2,8. Com exceção do rebanho situado no município de Turiúba, nos outros cinco foram encontrados animais reagentes ao vírus. Esta avaliação mostrou que houve circulação do vírus da Estomatite Vesicular nestes rebanhos provocando resposta humoral. Sendo assim é necessário a realização de estudos para se determinar o sítio de permanência do vírus.

In 2000 a total of 1099 serum samples were obtained of adult beef cattle, from 6 herds located at Auriflama, Gastão Vidigal,, Magda, Santo Antônio do Aracanguá, Sebastianópolis do Sul e Turiúba, counties from the region of Araçatuba. Sera were tested for neutralizing antibodies to vesicular stomatitis virus by microtiter neutralization assay, using the virus strain Indiana 1 Costa Rica/72 with 10(6,0) DICT50/50mL. The indicator cells were African green monkey kidney (VERO-CCL 81), purchased from American Type Cell Colection (ATCC). The neutralization titers of each serum were the reciprocal of the highest dilution expressed at log10 that protected 100.00% of the cell monolayer. Sera with values above 1,6 log10 (1/40) were considered positive for Vesicular Stomatitis virus. Twenty-eight (2.50%) out of 1.099 samples were positive for Vesicular Stomatitis virus Indiana 1, with antibodies titers ranging from 1.9 to 2.8. Except in Turiuba, in the others 5 herds there were positive animals. The presente survey showed the presence the Vesicular Stomatitis virus in the herds stimulating the humoral response. More studies are necessary to verify the site of persistence of the virus.

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