Resumo O objetivo deste trabalho foi avaliar crioprotetores na qualidade fisiológica de sementes de Adenium obesum preservadas em nitrogênio líquido. Foram utilizados os seguintes tratamentos: T1, controle, sem crioprotetor e sem imersão em nitrogênio líquido; T2, com imersão em nitrogênio líquido e sem crioprotetor; T3, com 0,4 mol L-1 de sacarose; T4, com 0,8 mol L-1 de sacarose; T5, com 1,0 mol L-1 de glicerol; T6, com 2,0 mol L-1 de glicerol com PVS1; T7, com PVS1; T8, com PVS2; T9, com PVS2 + floroglucinol (PVS2 modificado) e T10, com PVS3. As sementes de Adenium obesum apresentam tolerância à imersão e ao armazenamento em nitrogénio líquido, e não necessitam de crioprotectores para manter a qualidade fisiológica quando conservadas em água a 7,5%. tratamentos T1 T controle T2 T3 04 0 4 0, L1 L 1 L- sacarose T4 08 8 T5 10 1, T6 20 2 2, PVS1 PVS T7 T8 T9 (PVS modificado T10 PVS3 75 7 5 7,5% 7,5 7,

Abstract The objective of this work was to evaluate cryoprotectants on the physiological quality of Adenium obesum seeds preserved in liquid nitrogen. The following treatments were used: T1, control, without cryoprotectant and without immersion in liquid nitrogen; T2, with immersion in liquid nitrogen and without cryoprotectant; T3, with 0. 4 mol L-1 sucrose; T4, with 0.8 mol L-1 sucrose; T5, with 1.0 mol L-1 glycerol; T6, with 2.0 mol L-1 glycerol with PVS1; T7, with PVS1; T8, with PVS2; T9, with PVS2 + phloroglucinol (modified PVS2); and T10, with PVS3. Adenium obesum seeds show tolerance to immersion and storage in liquid nitrogen and do not need cryoprotectants to maintain their physiological quality when stored in a water content of 7.5%. used T1 T control T2 T3 0 L1 L 1 L- sucrose T4 08 8 T5 10 1. T6 20 2 2. PVS1 PVS T7 T8 T9 modified PVS2) T10 PVS3 75 7 5 7.5% 7.5 7.

Resumo Reguladores vegetais são alvos de estudos por terem a capacidade de modificar aspectos de interesse comercial nas plantas. É o caso do ácido giberélico (AG3), um dos hormônios responsáveis pelo crescimento e que está envolvido no processo de floração de muitos vegetais. Dessa maneira, o presente estudo buscou verificar os efeitos causados pela aplicação exógena de diferentes doses do regulador vegetal AG3 nos padrões florais, vegetativos e fisiológicos da orquídea Oncidium baueri. Os tratamentos experimentais (T1; T2; T3; T4; e T5) tiveram 10 repetições, cada uma contendo uma planta, com as respectivas doses pulverizadas: 0, 50, 100, 200 e 400 mg L-1 de AG3. Os parâmetros mensurados foram: comprimento (CP) e largura de pseudobulbo (LP), comprimento e largura de folhas (CF e LF), número de folhas por planta (NF), tempo para emissão de hastes florais (TEHF), tempo para abertura total (TATF) e tempo de queda de flores (TQF), com período mensurado em dias. O tamanho de haste floral (THF), o número de flores por haste (NFH), fluorescência da clorofila a (fv/fm) e a condutância estomática (G s ) também foram mensurados. A concentração de 200 mg L-1 de AG3 incrementou 3 cm na média em CP, CF. O NF apresentou tendência de aumento com o tratamento 4 em relação às doses diferentes de 0 mg L-1. Para os aspectos florais a mesma dose apresentou menor TEHF, sendo o único em destaque dentre os demais tratamentos. Para os parâmetros fisiológicos, essa mesma dose respondeu com maior valor de fv/fm (0,826) e G s (65,340 mmol m-2 s-1). Dessa maneira o uso de 200 mg L-1 de AG3 é recomendado em plantas de O. baueri para otimização da promoção vegetativa e floral. AG3, AG , (AG3) T1 T (T1 T2 T3 T4 T5 1 repetições pulverizadas 50 100 20 40 L1 L L- CP (CP LP, LP (LP) CF LF, LF LF) NF, (NF) TEHF (TEHF) TATF (TATF TQF, TQF (TQF) dias THF, THF (THF) NFH, NFH (NFH) fvfm fv fm (fv/fm L1. 1. 0,826 0826 826 (0,826 65,340 65340 65 340 (65,34 m2 m 2 m- s1. s1 . s-1) (AG3 (T 5 (LP (NF (TEHF (TQF (THF (NFH 0,82 082 82 (0,82 65,34 6534 6 34 (65,3 s-1 (AG 0,8 08 8 (0,8 65,3 653 (65, s- (0, 65, (65 (0 (6 (

Abstract Plant regulators are studied because they can alter commercially significant aspects of plant growth. Gibberellic acid (GA3) exemplifies this, as it plays a crucial role in the growth and flowering processes of various vegetables. Thus, the present study sought to verify the effects caused by the exogenous application of different doses of the plant regulator GA3 on the floral, vegetative, and physiological patterns of the orchid Oncidium baueri. The experimental treatments (T1; T2; T3; T4; and T5) had 10 replicates, each containing one plant, with the respective sprayed doses: 0, 50, 100, 200, and 400 mg L-1 of GA3. The parameters measured were: pseudobulb length (PL) and width (PW), leaf length (LL), leaf width (LW), number of leaves per plant (NL); time for flower spike to sprout (TFSS), time for flowers to open fully (TFOF), and time for flowers to fall (TFF), these measured in days; flower stem length (FSL), number of flowers per stem (NFS), chlorophyll a fluorescence (fv/fm), and stomatal conductance (G s ). Data were compared using the Tukey test at a significance of 5%. The concentration of 200 mg L-1 of GA3 increased PL and LL by 3 cm on average, and NL showed an increasing trend with treatment 4 compared to doses other than 0 mg L-¹. Regarding floral aspects, this same dose showed the lowest TFSS, distinguishing itself from the other treatments. For physiological parameters, this dose also resulted in higher fv/fm (0.826) and G s (65.340 mmol m-2 s-1). Therefore, the use of 200 mg L-1 of GA3 is recommended for O. baueri plants to optimize vegetative and floral promotion. GA (GA3 vegetables Thus T1 T (T1 T2 T3 T4 T5 1 replicates 50 100 40 L1 L L- (PL PW, PW , (PW) LL, (LL) LW, LW (LW) (NL) TFSS (TFSS) TFOF, TFOF (TFOF) TFF, TFF (TFF) days FSL, FSL (FSL) NFS, NFS (NFS) fv/fm, fvfm fv fm (fv/fm) . ) 5 5% 20 average L¹. L¹ ¹. ¹ L-¹ 0.826 0826 826 (0.826 65.340 65340 65 340 (65.34 m2 m 2 m- s1. s1 s-1) Therefore O promotion (GA (T (PW (LL (LW (NL (TFSS (TFOF (TFF (FSL (NFS (fv/fm 0.82 082 82 (0.82 65.34 6534 6 34 (65.3 s-1 0.8 08 8 (0.8 65.3 653 (65. s- 0. (0. 65. (65 (0 (6 (

RESUMO Relatar caso de infiltração corneana resistente ao tratamento em um paciente 4 meses após o retransplante da córnea. O diagnóstico etiológico foi estabelecido por microbiologia e microscopia confocal da córnea. Uma paciente de 80 anos de idade apresentou infiltrado estromal no botão corneano doador 4 meses após o retransplante da córnea. Inicialmente, a cultura da córnea era positiva para Candida parapsilosis. A microscopia confocal da córnea demonstrou micro-organismos de aproximadamente 4 μm esféricos e hiper-refletivos sugestivos de Candida spp. Uma segunda cultura de córnea foi positiva para Candida sp., e foi observada a presença de hifas no material analisado. A paciente permaneceu refratária à terapia. A microscopia confocal da córnea continua a crescer como um método de diagnóstico útil e não invasivo e no monitoramento da resposta à terapia. Este relato contribui por meio da detecção da Candida sp. na microscopia confocal da córnea para auxiliar no diagnóstico das ceratites. 8 Inicialmente parapsilosis microorganismos micro organismos hiperrefletivos hiper refletivos spp sp analisado terapia ceratites

ABSTRACT To describe a case of treatment-resistant corneal infiltrate in a patient 4 months after penetrating keratoplasty. The etiological diagnosis was established through microbiology and in vivo corneal confocal microscopy. A 80-year-old female patient presented with stromal infiltrate in the donor corneal bud 4 months following penetrating keratoplasty. Initially, the corneal culture was positive for Candida parapsilosis. In vivo corneal confocal microscopy demonstrated approximately 4 μm hyperreflective spherical microorganisms with a suggestive Candida spp. A second corneal culture was positive for Candida sp., and hyphae presence in the analyzed material was observed. The patient remained refractory to therapy. Confocal microscopy of the cornea continues to grow as a useful, non-invasive diagnostic method and in monitoring response to therapy. This report contributes by detecting Candida sp. in in vivo corneal confocal microscopy to aid in the diagnosis of keratitis. treatmentresistant treatment resistant keratoplasty 80yearold yearold 80 year old Initially parapsilosis spp sp observed therapy useful noninvasive non invasive keratitis 8

ABSTRACT Rickettsiosis is a disease caused by bacteria belonging to the genus Rickettsia, and is a potentially fatal zoonotic disease of great medical and veterinary importance. Given the urgent need to develop new diagnostic methods for detecting this disease, the present review aimed to evaluate the number of publications dedicated to the identification of Rickettsia spp. in human samples using molecular methods, such as polymerase chain reaction and its variations. To this end, a bibliographical survey covering articles published in the past ten years was conducted using the PudMed platform with the keywords “spotted fever” and “Rickettsia,” both combined with “diagnosis.” A growing number of publications in this area reflects an increasing interest in research, especially since 2015. From 2015 to February 2024, several promising results were tested and many studies were able to detect the genetic sequences of interest. Therefore, the absence of a standard diagnosis method highlights the critical need for developing an effective technique capable of accurately detecting the etiological agent and ensuring accurate diagnosis of the disease. importance spp variations end spotted fever “Rickettsia, diagnosis. “diagnosis. research 201 2024 Therefore “Rickettsia “diagnosis 20 202 2

Resumen Introducción Los adolescentes que practican deportes tienen mejores indicadores de salud mental. Objetivo Analizar la asociación entre los diferentes tipos de actividad física (ejercicio sistematizado, deportes individuales y colectivos), la salud mental y la ideación suicida en adolescentes. Método Se realizó un estudio transversal realizado con 666 adolescentes brasileños (14-19 años) que asisten a escuelas secundarias y fueron seleccionados mediante muestreo aleatorio por conglomerados. Los indicadores de salud mental analizados fueron evaluados a través del Cuestionario de Autoinforme y el Cuestionario de Fortalezas y Dificultades, incluyendo la ideación suicida. También obtuvimos información de la actividad física a través de cuestionarios. Resultados Hubo una mayor prevalencia relacionada con trastornos mentales (54.2% contra 32.5%), dificultades relacionadas con la salud mental (79.6% contra 48.4%) y pensamientos suicidas (22.9% contra 11.4%) en niñas que en niños (p < .001 para todos). Los chicos practicaban más deportes de equipo (41.0 contra 23.8), mientras que las chicas hacían más ejercicio (45.1 contra 26.5; p < .001). Discusión y conclusión La práctica de deportes de equipo ayuda en el desarrollo del espíritu colectivo, estimula la interacción social, desarrolla el razonamiento y la inteligencia emocional. Los niños que practican deportes de equipo tienen menos síntomas de trastornos mentales comunes, menos problemas de salud mental y menos ideación suicida en comparación con los niños físicamente inactivos.

Abstract Introduction Adolescents who practice sports have better mental health indicators. Objective To analyze the association between different types of physical activity (systematized exercise, individual, and collective sports), mental health, and suicidal ideation in adolescents. Method We conducted a cross-sectional study with 666 Brazilian adolescents (14-19 y.o.) attending high schools and selected using random cluster sampling. The mental health indicators analyzed were evaluated through the Self Reporting Questionnaire and the Strengths and Difficulties Questionnaire, including suicidal ideation. We also obtained physical activity information through questionnaires. Results There was a higher prevalence related to mental disorders (54.2% vs. 32.5%), difficulties related to mental health (79.6% vs. 48.4%), and suicidal thoughts (22.9% vs. 11.4%) in girls than in boys (p < .001 for all). The boys engaged in more team sports (41.0 vs. 23.8), whereas girls performed more exercise (45.1 vs. 26.5; p < .001). Discussion and conclusion The practice of team sports helps develop the collective spirit, stimulates social interaction, and develops reasoning and emotional intelligence. Boys who play team sports have fewer symptoms of common mental disorders, lower mental health problems, and less suicidal ideation than physically inactive boys.

ABSTRACT Introduction: The therapeutic plasma exchange (TPE) controls the systemic cytokine level and might improve the immune response in patients with severe Coronavirus (COVID-19) infection. To date, in developing countries, no study has explored the effectiveness and risk factors in a population with severe COVID-19 exposed to the TPE. Method: We described the risk factors associated with survival rates higher than 28 days and length of stay (LOS) in the intensive care unit (ICU) shorter than 15 days. Severe COVID-19 cases treated with TPE were included, from August 2020 to June 2021. Survival analysis with Kaplan-Meier curves, log-rank tests and multivariate logistic regressions were conducted to assess patient-related factors that could predict a higher survival rate and the ICU LOS. Results: A total of 99 patients with severe COVID-19 who had received TPE were followed during their hospital stay and for 28 days after discharge. The sample was composed of men (63%) aged 56 ± 16 years. The overall survival rate at 28 days was 80%. The ICU LOS (p = 0.0165) and mechanical ventilation (MV) (p = 0.00008) were considered factors that could increase the risk of death. Patient-related factors that influenced the 28-day mortality were the smoking status (OR = 5.8; 95%CI 1.5, 22) and history of oncologic or non-malignant hematologic diseases (OR = 5.9; 95%CI 1.2, 29). Conclusion: Patients with severe COVID-19 exposed to the TPE were associated with a 20% risk of death in a 28-day observation window, appearing to be lower than previous treatments. Active smoking, cancer and immunosuppressive conditions should be considered as relevant variables to be controlled in future trials on the TPE and COVID-19. Introduction (TPE COVID19 COVID 19 (COVID-19 infection date countries COVID-1 Method 2 (LOS (ICU 1 included 202 2021 KaplanMeier Kaplan Meier curves logrank log rank patientrelated patient related Results 9 discharge 63% 63 (63% 5 years 80 80% p 0.0165 00165 0 0165 MV (MV 0.00008 000008 00008 Patientrelated Patient 28day day OR 5.8 58 8 95CI CI 95 1.5 22 nonmalignant non malignant 5.9 59 12 1.2 29. 29 . 29) Conclusion 20 window treatments COVID19. 19. COVID1 (COVID-1 COVID- 6 (63 0.016 0016 016 0.0000 00000 0000 5. 1. (COVID- (6 0.01 001 01 0.000 000 (COVID ( 0.0 00 0.00 0.

Resumo O objetivo deste estudo foi avaliar a influência das formas clínica e subclínica da toxemia da prenhez (TP) na dinâmica dos metabólitos sanguíneos, compondo os perfis proteico, enzimático e mineral das cabras leiteiras durante o período de transição. Foram utilizadas 111 cabras multíparas que eram criadas em sistema intensivo. Grupos experimentais (n=3) foram criados utilizando diferentes concentrações séricas de βHB como ponto de corte, o G1 grupo controle (n=40), G2 grupo subclínico da TP (n=39) estabelecido quando pelo menos um dos momentos experimentais apresentou valores de βHB entre 0,8 mmol/L e 1,6 mmol/L; e o grupo G3, com manifestação clínica da TP (n=32), que em qualquer um dos momentos os valores de βHB foram superiores a 1,6 mmol/L. Os animais foram avaliados aos 30º, 20º e 10º dias antes do parto (dap), no parto e no 10º, 20º e 30º dia pós-parto (dpp). Proteínas totais (PT), albumina, globulina, ureia, creatinina, aspartato aminotransferase (AST), gama glutamiltransferase (GGT), creatina quinase (CK), amilase, fósforo, cloreto e cálcio, sódio e potássio foram mensurados. A análise de variância (Teste F) foi realizada com o objetivo de investigar os efeitos e interações entre os tempos dos grupos. A doença clínica e/ou subclínica resultou num aumento das concentrações de fósforo e potássio (P<0,05), foi observada uma diminuição nos valores de proteína total, albumina, globulina e cálcio ionizado (P<0,05). Não houve efeito estatístico da TP sobre a uréia, creatinina, AST, GGT, CK, amilase e sódio (P>0,05). A ocorrência da forma subclínica foi maior que a clínica durante o período de transição. As variáveis que compõem o perfil mineral foram as que apresentaram alterações decorrentes da TP, destacando-se, o cálcio ionizado. Chama-se a atenção o impacto que a doença reflete nesses componentes e na saúde do animal. (TP sanguíneos proteico transição 11 intensivo n=3 n3 n 3 (n=3 corte G n=40, n40 n=40 , 40 (n=40) n=39 n39 39 (n=39 08 0 8 0, mmolL mmol L 16 1 6 1, G3 n=32, n32 n=32 32 (n=32) º dap, dap (dap) pósparto pós dpp. dpp . (dpp) PT, PT (PT) albumina ureia creatinina AST (AST) GGT (GGT) CK (CK) mensurados Teste F grupos eou ou P<0,05, P005 P P<0,05 05 (P<0,05) total P<0,05. uréia P>0,05. P>0,05 (P>0,05) destacandose, destacandose destacando se, se destacando-se Chamase Chama animal n= (n= n4 n=4 4 (n=40 (n=32 (dap (dpp (PT (AST (GGT (CK P00 P<0,0 (P<0,05 P>0,0 (P>0,05 (n (n=4 P0 P<0, (P<0,0 P>0, (P>0,0 P<0 (P<0, P>0 (P>0, P< (P<0 P> (P>0 (P< (P> (P

Abstract The purpose of this study was to evaluate the influence of clinical and subclinical pregnancy toxemia (PT) forms on the dynamics of blood metabolites, composing the protein, enzymatic and mineral profiles of dairy goats during the transitional period. 111 multiparous dairy goats were used in this research. The animals were raised under an intensive system. Experimental groups (n=3) were created using the βHB blood concentrations as a cut-off point. The G1 or control group (n = 40), G2 or subclinical PT group (n = 39) were established when least one of the experimental assessment times presented βHB values between 0.8 mmol/L and 1.6 mmol/L; whereas G3 or clinical PT group (n = 32), which at any of the experimental assessment times of βHB values were higher than 1.6 mmol / L when verified and also presented clinical signs of PT. The animals were evaluated on the 30th , 20th and 10th day ante-partum (dap), at parturition and on the 10th, 20th and 30th day postpartum (dpp). Total proteins (TP), albumin, globulin, urea, creatinine, aspartate aminotransferase (AST), Gamma glutamiltransferase (GGT), creatine kinase (CK), amylase, phosphorus, chloride and calcium, sodium and potassium ions were measured. The analysis of variance (F Test) was performed in order to investigate the effects and interactions between group and assessment times. Clinical and/or subclinical disease during the transitional period resulted in an increase of phosphorus and potassium concentrations (P<0.05), while a decrease in total protein, albumin, globulin, and ionized calcium values was observed (P<0.05). No statistical effect of PT on urea, creatinine, AST, GGT, CK, amylase and sodium was observed (P>0.05). Except the ionized calcium and phosphorus, the levels of all the metabolites were influenced (P<0.05) by late pregnancy, parturition and lactation. The occurrence of the subclinical form was higher than the clinical form during the transitional period. Among the all variables studied, mineral profile were those that suffered alteration resulting from PT, highlighting the ionized calcium. Attention is drawn to the magnitude of the impact of disease on these components interfering in animal health. (PT protein 11 research system n=3 n3 n 3 (n=3 cutoff cut off point G 40, 40 40) 39 08 0 8 0. mmolL 16 1 6 1. 32, 32 32) th antepartum ante partum dap, dap (dap) dpp. dpp . (dpp) TP, TP (TP) albumin globulin urea creatinine AST (AST) GGT (GGT) CK (CK) measured F Test andor P<0.05, P005 P P<0.05 05 P<0.05. P>0.05. P>0.05 (P>0.05) (P<0.05 lactation studied health n= (n= 4 (dap (dpp (TP (AST (GGT (CK P00 P<0.0 P>0.0 (P>0.05 (P<0.0 P0 P<0. P>0. (P>0.0 (P<0. P<0 P>0 (P>0. (P<0 P< P> (P>0 (P< (P> (P

Resumo Objetivou-se avaliar os efeitos do cetorolaco de trometamina a 0,5% e do diclofenaco de sódico a 0,1% sem conservantes na córnea de coelhos. Dezessete coelhos da raça Nova Zelândia foram aleatoriamente divididos em três grupos: o grupo de 0,5% de cetorolaco de trometamina, o grupo de 0,1% de diclofenaco sódico e o grupo controle (0,9% de NaCl). Para cada coelho, os dois olhos foram tratados três vezes ao dia durante 90 dias de acordo com o grupo de tratamento. Os epitélios da córnea foram analisados usando microscopia eletrônica de varredura para observar o número de células claras, cinzas e escuras, o número de criptas e a perda do formato celular hexagonal. Ambas as formulações administradas causaram alterações no epitélio da córnea de coelhos. Com exceção da contagem de criptas (p <0,05), todos os parâmetros apresentaram diferença estatisticamente significante entre os grupos. O número de células escuras foi maior no grupo cetorolaco de trometamina (p <0,05). O número de células cinzentas foi maior no grupo diclofenaco de sódio do que no grupo controle (p=0,003). O maior número de células escuras observado foi associado ao menor número de células claras (r=-0,577, p<0,001). A perda do formato celular mostrou uma correlação direta com o número de células escuras (r=0,524, p=0,002). O cetorolaco de trometamina 0,5% foi mais tóxico para o epitélio da córnea de coelhos do que o diclofenaco de sódio a 0,1%. Objetivouse Objetivou se 05 0 5 0,5 01 1 0,1 grupos 0,9% 09 9 (0,9 NaCl. NaCl . NaCl) coelho tratamento hexagonal p <0,05, 005 <0,05 , <0,05) <0,05. p=0,003. p0003 p=0,003 003 (p=0,003) r=0,577, r0577 r r= 0,577, 577 (r=-0,577 p<0,001. p0001 p<0,001 001 p<0,001) r=0,524, r0524 524 (r=0,524 p=0,002. p0002 p=0,002 002 p=0,002) 0, 0,9 (0, 00 <0,0 p000 p=0,00 (p=0,003 r=0,577 r057 0577 0,577 57 (r=-0,57 p<0,00 r=0,524 r052 52 (r=0,52 (0 <0, p00 p=0,0 (p=0,00 r=0,57 r05 057 0,57 (r=-0,5 p<0,0 r=0,52 (r=0,5 ( <0 p0 p=0, (p=0,0 r=0,5 r0 (r=-0, p<0, (r=0, < p=0 (p=0, r=0, (r=-0 p<0 (r=0 p= (p=0 r=0 (r=- p< (r= (p= (r

Abstract This study aimed to evaluate the corneal epitheliotoxic effects of preservative-free ketorolac tromethamine 0.5% and diclofenac sodium 0.1% eye drops in rabbits. Seventeen New Zealand rabbits were randomly divided into three groups: the 0.5% ketorolac tromethamine group, the 0.1% diclofenac sodium group, and the control group (0.9% NaCl). For each rabbit, both eyes were treated three times daily according to their treatment group. The corneal epithelia were analyzed using scanning electron microscopy to observe the number of light, grey, and dark cells; the number of epithelial holes; and the loss of hexagonal shape. Both of the formulations administered caused changes in the healthy corneal epithelia of rabbits. Except for number of epithelial holes (p < 0.05), all the parameters showed a statistically significant difference between the groups. The number of dark cells was highest in the ketorolac tromethamine group (p<0.05). The number of grey cells was higher in the diclofenac sodium group than in the control group (p =0.003). A higher number of dark cells was associated with a smaller number of light cells (r =-0.577, p < 0.001). Loss of shape showed a direct correlation with the number of dark cells (r=0.524, p=0.002). Based on the results presented, it was possible to conclude that ketorolac tromethamine 0.5% was more toxic to rabbit corneal epithelium than diclofenac sodium 0.1%. preservativefree preservative free 05 0 5 0.5 01 1 0.1 groups 0.9% 09 9 (0.9 NaCl. NaCl . NaCl) 0.05, 005 0.05 , 0.05) p<0.05. p005 p<0.05 (p<0.05) =0.003. 0003 =0.003 003 =0.003) r =0.577, 0577 = 0.577, 577 =-0.577 0.001. 0001 0.001 001 0.001) r=0.524, r0524 524 (r=0.524 p=0.002. p0002 p=0.002 002 p=0.002) presented 0. 0.9 (0. 00 0.0 p00 p<0.0 (p<0.05 000 =0.00 =0.577 057 0.577 57 =-0.57 0.00 r=0.524 r052 52 (r=0.52 p000 p=0.00 (0 p0 p<0. (p<0.0 =0.0 =0.57 0.57 =-0.5 r=0.52 r05 (r=0.5 p=0.0 ( p<0 (p<0. =0. =0.5 =-0. r=0.5 r0 (r=0. p=0. p< (p<0 =0 =-0 r=0. (r=0 p=0 (p< =- r=0 (r= p= r=

RESUMO A aroeira-do-sertão é uma espécie nativa do Brasil que está sendo explorada de forma predatória. Como a população desta espécie vem sendo reduzida, técnicas de preservação são fundamentais, como a conservação em nitrogênio líquido (NL). Objetivou-se avaliar diferentes soluções crioprotetoras na criopreservação em NL (-196 °C) de sementes de aroeira-do-sertão. Os tratamentos foram: controle (7,5±1,5 ° C); NL sem crioprotetor; sacarose 0,4 mol L-1; sacarose 0,8 mol L-1; glicerol 1 mol L-1; glicerol 2 mol L-1; PVS1 (solução de vitrificação de plantas); PVS2; PVS2 + 1% floroglucinol e PVS3. As sementes permaneceram congeladas por 120 dias. Antes da criopreservação, as sementes foram caracterizadas quanto à germinação e teor de água. Ao sétimo dia avaliaram-se diversos parâmetros de germinação e após 150 dias de cultivo, variáveis de crescimento das plantas. As sementes apresentaram 9,2% de teor de água e 74% de germinação antes da criopreservação. No controle, a germinação foi de 55%, enquanto na criopreservação, variou de 61 a 69%, mostrando efeito positivo da criopreservação, mesmo sem o uso de crioprotetores (69%). Observou-se modelo trifásico de absorção de água nas sementes, onde o NL promoveu antecipação da germinação, que se iniciou com 56 horas, enquanto o controle iniciou a germinação após 66 horas. As plantas após 150 dias de cultivo apresentaram desenvolvimento satisfatório de suas partes conforme verificado pelo índice de qualidade de Dickson (IQD). O uso de crioprotetores não influencia a germinação e crescimento inicial das plantas. As sementes de aroeira-do-sertão com 9,2% de teor de água podem ser criopreservadas em NL sem crioprotetores.

ABSTRACT Aroeira-do-sertão is a Brazilian native species that has been widely explored. Thus, the population of this species has been reduced and techniques for its preservation are essential, such as the conservation of seeds in liquid nitrogen (LN). The objective of this work was to evaluate different cryoprotectant solutions for cryopreservation of aroeira-do-sertão seeds in LN (-196 °C). The treatments used were: control (7.5±1.5 °C); LN without cryoprotectant; sucrose 0.4 mol L-1; sucrose 0.8 mol L-1; glycerol 1 mol L-1; glycerol 2 mol L-1; PVS1 (plant vitrification solution); PVS2; PVS2 + 1% phloroglucinol; and PVS3. The seeds remained frozen for 120 days. The seeds were evaluated for germination and water content before cryopreservation. Several germination parameters were evaluated on the seventh day and plant growth variables were evaluated after 150 days. The seeds presented 9.2% water content and 74% germination before cryopreservation. The germination in the control treatment was 55%, whereas it varied from 61% to 69% under cryopreservation, denoting the positive effect of cryopreservation, even without cryoprotectants (69%). The seeds presented a triphasic water absorption model; the LN accelerated the germination, which started within 56 hours, whereas the germination in the control treatment started after 66 hours. The plant parts presented satisfactory development after 150 days, as shown by the Dickson quality index. The use of cryoprotectants did not affect seed germination and initial growth of seedlings. Aroeira-do-sertão seeds with 9.2% water content can be cryopreserved in LN without cryoprotectants.

RESUMEN Objetivo. El objetivo fue evaluar el efecto de la variabilidad de las lluvias sobre la composición química, la degradabilidad in vitro y la producción de gas de cactus en la región Semiárida. Materiales y métodos. Este fue un diseño experimental completamente al azar en un arreglo factorial de 2 períodos de lluvia (lluvia alta y baja) x 5 especies de cactus, con 5 repeticiones. Los cactus evaluados fueron Pilosocereus gounellei (Weber ex K. Schum). Bly ex Rowl, Cereus jamacaru DC., Opuntia ficus indica Mill, Nopalea cochenillifera Salm. Dyck y Opuntia stricta (Haw.) Haw, todas frescas. Resultados. Hubo una interacción entre la variación de la lluvia y las especies de cactus para materia seca, proteína cruda, fibra detergente neutra y fibra detergente ácido (p<0.05), producción de gas in vitro (p<0.05) y para la degradabilidad in vitro de materia seca (p<0.05). Entre los cactus, Pilosocereus gounellei presentó una menor proporción de materia orgánica y un aumento en el contenido de materia mineral en relación a los demás cactus (p<0.05). Conclusiones. La composición química, los coeficientes de producción de gas y la degradabilidad in vitro fueron influenciados por las especies de cactus y por la variación de las precipitaciones. Las especies de cactus aumentan el contenido de materia seca, proteína cruda y fibra de detergente neutro durante la escasez de precipitaciones. Las variedades de nopal (Opuntia y Nopalea) se destacaron entre los cactus ensayados en relación a la producción de gas y la degradabilidad in vitro.

ABSTRACT Objective. The objective was to evaluate the effect of rainfall variability on the chemical composition, in vitro degradability and gas production of cacti in Semiarid region. Materials and methods. A completely randomized experimental design in a factorial arrangement of 2 rainfall periods (high and low rainfall) x 5 cactus species, with 5 replications was adopted. Cacti evaluated were Pilosocereus gounellei (Weber ex K. Schum). Bly ex Rowl, Cereus jamacaru DC., Opuntia ficus indica Mill, Nopalea cochenillifera Salm. Dyckand Opuntia stricta (Haw.) Haw, all fresh. Results. There was an interaction between rainfall variation and cactus species for dry matter, crude protein, neutral detergent fiber and acid detergent fiber (p<0.05), in vitro gas production (p<0.05) and for in vitro dry matter degradability (p<0.05). Among cacti, Pilosocereus gounellei presented a lower proportion of organic matter and an increase in the content of mineral matter in relation to the other cacti (p<0.05). Conclusions. The chemical composition, the gas production coefficients and the in vitro degradability were influenced by the cacti species and by the rainfall variation. Cacti species increase the dry matter, crude protein and neutral detergent fiber contents during the low rainfall. Varieties of cactus pear (Opuntia and Nopalea) stood out among the cacti tested in relation to gas production and in vitro degradability.

RESUMO Objetivos: Há diversas terapias sendo utilizadas ou propostas para a COVID-19, muitas carecendo de apropriada avaliação de efetividade e segurança. O propósito deste documento é elaborar recomendações para subsidiar decisões sobre o tratamento farmacológico de pacientes hospitalizados com COVID-19 no Brasil. Métodos: Um grupo de 27 membros, formado por especialistas, representantes do Ministério da Saúde e metodologistas, integra essa diretriz. Foi utilizado o método de elaboração de diretrizes rápidas, tomando por base a adoção e/ou a adaptação de recomendações a partir de diretrizes internacionais existentes (GRADE ADOLOPMENT), apoiadas pela plataforma e-COVID-19 RecMap. A qualidade das evidências e a elaboração das recomendações seguiram o método GRADE. Resultados: Foram geradas 16 recomendações. Entre elas, estão recomendações fortes para o uso de corticosteroides em pacientes em uso de oxigênio suplementar, para o uso de anticoagulantes em doses de profilaxia para tromboembolismo e para não uso de antibacterianos nos pacientes sem suspeita de infecção bacteriana. Não foi possível fazer uma recomendação quanto à utilização do tocilizumabe em pacientes hospitalizados com COVID-19 em uso de oxigênio, pelas incertezas na disponibilidade e de acesso ao medicamento. Foi feita recomendação para não usar azitromicina, casirivimabe + imdevimabe, cloroquina, colchicina, hidroxicloroquina, ivermectina, lopinavir/ ritonavir, plasma convalescente e rendesivir. Conclusão: Até o momento, poucas terapias se provaram efetivas no tratamento do paciente hospitalizado com COVID-19, sendo recomendados apenas corticosteroides e profilaxia para tromboembolismo. Diversos medicamentos foram considerados ineficazes, devendo ser descartados, de forma a oferecer o melhor tratamento pelos princípios da medicina baseada em evidências e promover economia de recursos não eficazes.

ABSTRACT Objective: Several therapies are being used or proposed for COVID-19, and many lack appropriate evaluations of their effectiveness and safety. The purpose of this document is to develop recommendations to support decisions regarding the pharmacological treatment of patients hospitalized with COVID-19 in Brazil. Methods: A group of 27 experts, including representatives of the Ministry of Health and methodologists, created this guideline. The method used for the rapid development of guidelines was based on the adoption and/or adaptation of existing international guidelines (GRADE ADOLOPMENT) and supported by the e-COVID-19 RecMap platform. The quality of the evidence and the preparation of the recommendations followed the GRADE method. Results: Sixteen recommendations were generated. They include strong recommendations for the use of corticosteroids in patients using supplemental oxygen, the use of anticoagulants at prophylactic doses to prevent thromboembolism and the nonuse of antibiotics in patients without suspected bacterial infection. It was not possible to make a recommendation regarding the use of tocilizumab in patients hospitalized with COVID-19 using oxygen due to uncertainties regarding the availability of and access to the drug. Strong recommendations against the use of hydroxychloroquine, convalescent plasma, colchicine, lopinavir + ritonavir and antibiotics in patients without suspected bacterial infection and also conditional recommendations against the use of casirivimab + imdevimab, ivermectin and rendesivir were made. Conclusion: To date, few therapies have proven effective in the treatment of hospitalized patients with COVID-19, and only corticosteroids and prophylaxis for thromboembolism are recommended. Several drugs were considered ineffective and should not be used to provide the best treatment according to the principles of evidence-based medicine and promote economical resource use.

Resumo O presente estudo teve como objetivo buscar evidências de validade para a Escala de Autoeficácia para Regulação Emocional (AERE) por meio da relação com a Escala de Autorregulação Emocional (EARE) e a Escala de Autoeficácia Social (AES). Para tanto, 549 participantes com idades entre 13 e 23 anos (M = 16,82, DP = 1,551), de Belém (72,7%, n = 399) e do Rio de Janeiro (27,3%, n = 150) compuseram a amostra. Eles responderam a EARE e outros dois instrumentos, um que avalia a autorregulação emocional diante da tristeza (Escala de Autorregulação Emocional - EARE) e outro, que avalia a autoeficácia social (Escala de Autoeficácia Social - AES). Os resultados indicaram que a autorregulação emocional mostrou maiores índices de predição em relação à autoeficácia para regulação emocional, do que para a autoeficácia social, embora ambos os construtos tenham gerado coeficientes significativos. Os achados corroboram a literatura, confirmando a evidência de validade para a Escala de Autoeficácia para Regulação Emocional e são discutidos à luz dos pressupostos teóricos.

Abstract This study aimed to seek evidence of validity for the Self-Efficacy Scale for Emotional Regulation (SESER) using the relationship with the Emotional Self-Regulation Scale (ESRE) and the Social Self-Efficacy Scale (SSES). Participants included a total of 549 students aged between 13 and 23 years old (M = 16.82, SD = 1.551) from the Brazilian municipalities of Belém (72.7%, n = 399) and Rio de Janeiro (27.3%, n = 150). They responded to SEERS and two other instruments, one that assessed emotional self-regulation in the face of sadness (Emotional Self-Regulation Scale - ESRS) and another that assessed social self-efficacy (Social Self-Efficacy Scale - SSE). The results indicated that emotional self-regulation showed higher rates of prediction for emotion regulation self-efficacy than for social self-efficacy, although both constructs generated significant coefficients. The findings corroborate the literature, confirming the evidence of validity for the Self-efficacy Scale for Emotional Regulation and are discussed in the light of theoretical assumptions.

Resumen El presente estudio tuvo como objetivo buscar evidencias de validez para la Escala de Autoeficacia para la Regulación Emocional (AERE) a través de la relación com la Escala de Autorregulación emocional (EARE) y la Escala de auto eficacia social (AES). Com este fin, 549 participantes com edades comprendidas entre 13 y 23 años (M = 16.82, DS = 1.551), de las ciudades brasileñas de Belém (72.7%, n = 399) y de Río de Janeiro (27.3%, n = 150) formaron la muestra. Respondieron al EARE y a otros dos instrumentos, uno que evalúa la autorregulación emocional frente a la tristeza (Escala de Autorregulación Emocional - EARE) y com que evalúa la autoeficacia social (Escala de Autoeficacia Social - AES). Los resultados indicaron que la autorregulación emocional mostró mayores índices de predicción com relación com la autoeficacia para la regulación emocional que para la autoeficacia social, aunque ambos constructos generaron coeficientes significativos. Los hallazgos corroboran la literatura, confirmando la evidencia de validez de la Escala de Autoeficacia para la Regulación Emocional y se discuten a la luz de los supuestos teóricos.

Resumo A bullosis diabeticorum (BD) é uma manifestação cutânea infrequente do diabetes e pode afetar os membros superiores. Caracteriza-se pelo aparecimento espontâneo e indolor de flictenas não inflamatórias de aspecto sanguinolento, que podem evoluir para necrose, requerendo que se faça diagnóstico diferencial com doenças dermatológicas, como porfiria cutânea tarda, pseudoporfiria, epidermólise bolhosa adquirida e penfigoide bolhoso, e doenças vasculares, como vasculites, doença arterial periférica, doença de Buerger, entre outras. Neste relato, descreve-se o caso de um paciente masculino de 77 anos, hipertenso e diabético mal controlado, apresentando aparecimento espontâneo de lesões bolhosas de membros superiores, que evoluíram, após ruptura espontânea, para ulcerosas necróticas. A biópsia da lesão diagnosticou a presença de espessamento hialino de vasos dérmicos e de bolhas subcórneas, compatíveis com o diagnóstico de BD. Após a interrupção do tabagismo e a otimização do controle glicêmico associado a corticoterapia tópica, houve melhora, com cicatrização das lesões. A apresentação da BD em membros superiores é mais rara, sendo necessário o diagnóstico diferencial com outras lesões cutâneas e vasculares.

Abstract Bullosis diabeticorum (BD) is an uncommon cutaneous manifestation of diabetes that can affect the upper limbs. It is characterized by spontaneous and painless non-inflammatory bloody blisters, which can progress to necrosis, requiring differential diagnosis to rule out other dermatological diseases, such as porphyria cutanea tarda, pseudoporphyria, epidermolysis bullosa acquisita, and pemphigoid, and vascular diseases, such as vasculitis, peripheral arterial disease, and Buerger’s disease, among others. In this report, we describe a 77-year-old male patient with poorly controlled diabetes and hypertension who presented with spontaneous onset of lesions on the upper limbs, initially with bullous characteristics, progressing to necrotic ulcers after spontaneous rupture. A biopsy revealed hyaline thickening of the dermal vessels and subcorneal bullae, consistent with a diagnosis of BD. After smoking cessation and optimization of glycemia control combined with topical corticosteroid therapy, the condition improved and lesions began to heal. This presentation of BD involving the upper limbs is rare, requiring differential diagnosis to rule out other cutaneous and vascular lesions.

Resumo O carcinoma anaplásico da tireoide corresponde ao câncer de menor incidência e de pior prognóstico. Neste relato, descrevemos o caso de uma paciente de 64 anos submetida a tireoidectomia total associada a esvaziamento cervical nível VI devido a carcinoma papilífero de tireoide. No seguimento, apresentou sinais de recidiva regional e foi submetida a esvaziamento cervical ampliado e esofagectomia cervical. No intraoperatório, observou-se ausência de plano de clivagem entre o tumor e a artéria carótida comum (ACC) e procedeu-se com implante de shunt carotídeo, resseção em bloco incluindo segmento da ACC e do esôfago acometidos e ponte vascular com interposição da veia safena magna. Foi evidenciado carcinoma anaplásico em revisão de lâmina do exame anatomopatológico. A paciente foi submetida ao tratamento adjuvante e não apresenta sinais de recidiva locorregional. Diante da possibilidade de proceder uma cirurgia curativa com ressecção em bloco, o cirurgião vascular deve estar apto para as opções cirúrgicas.

Abstract Of all thyroid cancers, anaplastic thyroid carcinoma (ATC) has the lowest incidence and worst prognosis. In this report, we describe a 64-year-old female patient who underwent total thyroidectomy and level VI neck dissection for papillary thyroid carcinoma. During follow-up, she showed signs of regional recurrence and underwent extended neck dissection and cervical esophagectomy. Intraoperatively, there was no cleavage plane between the tumor and the common carotid artery (CCA), so a carotid shunt was implanted and en bloc resection, including the affected CCA and esophagus segments was performed followed by vascular bypass with interposition of a great saphenous vein graft. A pathology review found evidence of anaplastic carcinoma. The patient underwent adjuvant treatment and has no signs of locoregional recurrence. Presented with the possibility of carrying out curative surgery with en bloc resection, the vascular surgeon must be prepared for the surgical options.

ABSTRACT. This study aimed to assess the chemical responses of forage watermelon fruit at different maturity stages or storage lengths, performing two experimental tests. In the first test, four maturity stages were assessed: 30, 45, 60, and 75 days after anthesis, with four replicates. In the second test, fruits were maintained under three storage lengths: T1D (harvest day), T3M (3 months after harvest), and T6M (6 months after harvest), with eight replicates. Experimental design was completely randomized in both experimental tests. Fruit maturity stage did not affect crude protein, total carbohydrate, neutral detergent fiber, in vitro dry matter digestibility (IVDMD), pulp firmness, soluble solids content and total pectin content, but increased acid detergent fiber content from 45 days after anthesis. Storage length up to six months after harvest increased ash, crude protein and IVDMD, and reduced the content of soluble solids. Forage watermelon fruit can be harvested from 30 to 75 days after anthesis equivalent to 75 - 120 days after planting, and they can be stored under tree shade up to 6 months after harvest.