ABSTRACT Coffee is a beverage that is widely consumed throughout the world, especially in Brazil. Its high market value makes it a prime target for fraud. To combat this fraud, different methods have been developed and improved over the years to detect potential adulterants in coffee and guarantee its authenticity. Nevertheless, these methods still pose a challenge. Therefore, this work aimed to identify adulteration markers in roasted and ground Arabica coffee using high-performance liquid chromatography (HPLC) to determine the flavonoid and phenolic acid profile. Samples of Arabica coffee beans and defective coffee beans were adulterated with coffee husks by preparing mixtures in different proportions (97:3, 90:10, 80:20 % w/w), which were then subjected to chromatographic and chemometric analyses, including principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA). Using the phenolic compounds associated with chemometric methods, protocatechuic acid was identified as a marker of adulteration in roasted and ground coffee with Arabica coffee husks, even at low adulterant concentrations (3 %). world Brazil fraud authenticity Nevertheless challenge Therefore highperformance performance HPLC (HPLC profile 973, 973 97 3, 3 (97:3 9010, 9010 90 10, 10 90:10 8020 80 20 80:2 w/w, ww w/w , w w/w) analyses PCA (PCA PLSDA. PLSDA PLS DA . (PLS-DA) ( %. %) 9 (97: 901 1 90:1 802 8 2 80: (PLS-DA (97 90: (9

Abstract The symbiosis between arbuscular mycorrhizal fungi (AMF) and plants represents an important alternative to increase yields in agricultural crops. The objective of the study was to evaluate the propagation of AMF isolated from soils with different management, and inoculated in Cucurbita pepo var. “Grey Zucchini.” A trap culture was established as a means of AMF propagation and a block design was used (five treatments x 15 repetitions). The native AMF came from three systems (T1 = secondary vegetation; T2 = multipurpose system; T3 = agricultural system). A positive (CP = GLUMIX®MICORRIZAS) and negative (CN = no inoculation) control were used. Mycorrhizal inoculation began in the seedbed, using Peat Moss-sterile soil (50:50 v/v). The substrate method with 12.5 g of native inoculant (T1 = 46 spores; T2 = 32 spores and T3 = 22 spores) was used as the inoculation method. In CP, 5 g of product = 100 spores were used. The results indicate that the greatest mycorrhizal propagation occurred in CP and T1, with a spore multiplication of 135.20 ± 26.24 and 74.67 ± 5.56 spores in 10 g of soil, and identifying a native consortium made up of AMF spores from the Glomaceae and Gigasporacea families. In conclusion, C. pepo represents a promising host for the trapping and multiplication of AMF.

Resumen La simbiosis entre los hongos micorrízícos arbusculares (HMA) y las plantas, representa una alternativa importante para incrementar los rendimientos en los cultivos agrícolas. El objetivo del estudio fue evaluar la propagación de HMA aislados de suelos con diferente manejo e inoculado en Cucurbita pepo var. “Grey Zucchini”. Se estableció un cultivo trampa como medio de propagación de HMA. Se empleó un diseño en bloques (cinco tratamientos x15 repeticiones). Los HMA nativos fueron provenientes de tres sistemas (T1 = vegetación secundaria; T2 = sistema multipropósito; T3 = sistema agrícola). Se usó un control positivo (CP = GLUMIX®MICORRIZAS) y control negativo (CN = sin inoculación). La inoculación micorrízica inició en semillero, empleando Peat Moss-suelo estéril (50:50 v/v). Se empleó como método de inoculación el método por sustrato con 12.5 g de inoculante nativo (T1 = 46 esporas; T2 = 32 esporas y T3 = 22 esporas). En CP empleó 5 g de producto 100 esporas. Los resultados indican que la mayor propagación micorrízica se presentó en el CP y T1, con una multiplicación de esporas de 135.20 ± 26.24 y 74.67 ± 5.56 esporas en 10 g de suelo. Identificando un consorcio nativo conformado de esporas de HMA de las familias Glomaceae y Gigasporacea. En conclusión, C. pepo representa un prometedor hospedero para el trampeo y multiplicación de los HMA.

Abstract One physical modification (autoclaving) and five chemical modifications (lintnerization, crosslinking, acetylation, hydroxypropylation, and oxidation) were performed on the native oat starch. The starches were mixed with chitosan (Ch) and plasticizer (glycerol) to make films. The film-forming solutions (FFS) were characterized rheologically and evaluated for physical properties (color, thickness, humidity and solubility), water vapor and oxygen permeability (10 and 25 °C), and mechanical properties. Antibacterial activity was evaluated against Escherichia coli, Staphylococcus aureus and Listeria monocytogenes by the agar diffusion. The functionality of the films as packaging in ground pork was evaluated by counting fecal coliforms and total aerobes for 21 days at 4°C. All the FFS presented a pseudoplastic-type behavior. The addition of Ch increased the stress at fracture (TF) and decreased the percentage elongation (% E) in all the films; being the Oxidized-Ch formulation the one that presented the greatest increase (≈ 200 %) in TF and the smallest decrease (38 %) in % E. All formulations with Ch presented antibacterial activity, which was higher in the Oxidized-Ch film and decreased with the evaluation time. The results generally indicated that the Oxidized-Ch film may represent a suitable formulation as antibacterial packaging with adequate physical, mechanical, and barrier properties.

Resumen Se realizó una modificación física (autoclaveado) y cinco modificaciones químicas (lintnerización, entrecruzamiento, acetilación, hidroxipropilación y oxidación) al almidón nativo de avena. Los almidones se mezclaron con quitosano (Q) y plastificante (glicerol) para elaborar películas. Las soluciones formadoras de película (SFP) se caracterizaron reológicamente. En las películas se evaluaron las propiedades físicas (color, grosor, humedad y solubilidad), la permeabilidad al vapor de agua y al oxígeno (10 y 25 °C) y las propiedades mecánicas. Se evaluó la actividad antibacteriana frente a Eschericia coli, Staphylococcus aureus y Listeria monocytogenes mediante el método de difusión en agar. La funcionalidad de las películas como envase de carne picada de cerdo se evaluó mediante el recuento de coliformes fecales y aerobios totales durante 21 días a 4°C. Todas las SFP presentaron comportamiento pseudoplástico. La adición de Q aumentó la tensión a la fractura (TF) y disminuyó el porcentaje de elongación (% E) en todas las películas; siendo la formulación Oxidado-Q la que presentó el mayor incremento (≈ 200 %) en TF y la menor disminución (38 %) en %E. Todas formulaciones con Q presentaron actividad antibacterial, la cual fue mayor en la película Oxidado-Q y disminuyó con el tiempo de evaluación. En general, los resultados indicaron que la película Oxidado-Q puede representar una formulación conveniente como envase antibacteriano con adecuadas propiedades físicas, mecánicas y de barrera.

Abstract The acceptance of modern myths about sexual aggression (AMMSA) scale is used in several countries, as gender-based violence increases significantly, especially after the pandemic, so it is crucial to understand its psychometric properties in different populations, as in this case with a Peruvian sample. This included 743 participants, divided into two groups: those with no recorded history of violence and those with a recorded history of intimate partner violence. Two methods were used to assess the factor structure of the AMMSA, exploratory factor analysis (EFA), followed by confirmatory factor analysis (CFA), to validate the structure of the scale. The results revealed a unidimensional 23-item model, which demonstrated the best parsimony and theoretical fit, supported by an alpha coefficient of 0.90. The invariance between the tested groups (gender and university) was adequate, and the assessment of convergent validity psychometrically supported the proposed model. The cut-off point was set at 65, with an area under the curve of 86.7 %. Consequently, a score ≥ 65 indicated the presence of these myths. The satisfactory results of the AMMSA allow us to evaluate and distinguish myths about sexual aggression in the Peruvian population.

Resumo A escala de aceitação dos mitos modernos sobre violência sexual (acceptance of modern myths about sexual aggression (AMMSA)) e usada em vários países, pois a violência de genero aumenta significativamente, especialmente após a pandemia, por isso e fundamental entender suas propriedades psicométricas em diferentes populações, como, neste caso, com uma amostra peruana. Essa amostra incluiu 743 participantes, divididos em dois grupos: aqueles sem histórico registrado de violência e aqueles com histórico registrado de violência por parceiro íntimo. Foram usados dois métodos para avaliar a estrutura fatorial da AMMSA: análise fatorial exploratória, seguida de análise fatorial confirmatória, para validar a estrutura da escala. Os resultados revelaram um modelo unidimensional de 23 itens, que demonstrou a melhor parcimônia e ajuste teórico, apoiado por um coeficiente alfa de 0,90. A invariância entre os grupos testados (gênero e universidade) foi adequada, e a avaliação da validade convergente apoiou psicometricamente o modelo proposto. O ponto de corte foi definido em 65, com uma área sob a curva de 86,7 %. Consequentemente, uma pontuação ≥ 65 indicou a presença desses mitos. Os resultados satisfatórios da AMMSA nos permitem avaliar e distinguir os mitos sobre violência sexual na população peruana

Resumen La escala de aceptación de mitos modernos sobre la agresión sexual (acceptance of modern myths about sexual aggression (AMMSA)) se utiliza en diversos países, ya que la violencia de género aumenta significativamente, sobre todo después de la pandemia, por lo que es crucial comprender sus propiedades psicométricas en distintas poblaciones, como en este caso con una muestra peruana. Esta incluyó a 743 participantes, divididos en dos grupos: aquellos sin antecedentes registrados de violencia y aquellos con antecedentes registrados de violencia de pareja. Se emplearon dos métodos para evaluar la estructura factorial de la AMMSA, el análisis factorial exploratorio (AFE), seguido del análisis factorial confirmatorio (AFC), para validar la estructura de la escala. Los resultados revelaron un modelo unidimensional de 23 ítems, que demostró la mejor parsimonia y ajuste teórico, respaldado por un coeficiente alfa de 0.90. La invarianza entre los grupos evaluados (sexo y universidad) fue adecuada y la evaluación de la validez convergente respaldó psicométricamente el modelo propuesto. El punto de corte se estableció en 65, con un área bajo la curva del 86.7 %. En consecuencia, una puntuación ≥ 65 indica la presencia de estos mitos. Los resultados satisfactorios de la AMMSA permiten evaluar y distinguir los mitos sobre la agresión sexual en la población peruana.

ABSTRACT Objective: To determine the standard surgical times of the four most common general surgery procedures (unilateral inguinal hernioplasty, bilateral inguinal hernioplasty, umbilical hernioplasty and cholecystectomy) in a second-level hospital and to estimate the probability of extending the time of each of the procedures. Efficiency is a widely studied subject in economics. It involves the need for fewer elements in the production of a certain level of goods and services. Therefore, it is extremely important to consider it in the field of surgery. Materials and methods: An observational, descriptive and retrospective study. It used the operating room records from 2017 to 2019 of the General Surgery service in a second-level hospital. Based on this information, the time required for each procedure was standardized using the mean for each one (umbilical hernioplasty, unilateral or bilateral inguinal hernioplasty and cholecystectomy). The probability of extending surgical times was estimated based on the obtained data and confidence interval. Results: The mean for unilateral inguinal hernioplasty was 76 min (95.00 % CI: 72-80 min, SD 23), for bilateral inguinal hernioplasty 104.38 min (95.00 % CI: 91-116 min, SD 41.7), for umbilical hernioplasty 59.31 min (95.00 % CI: 54-63 min, SD 29.99) and for cholecystectomy 85.735 min (95.00 % CI: 83-88 min). The probability of scheduling three surgical interventions and completing all of them on time (upper limit of the CI) is 92.69 %, and the probability of scheduling three surgical interventions and extending the time of all of them is 0.0016 % (lower limit of the CI). Conclusions: Planning scheduled operations using standardized surgical times is feasible. Updated statistics on surgical procedures (average time for each procedure) are required since it is possible to more accurately detect and supervise operating room dynamics by identifying opportunity areas. This will make operating room time more efficient for the benefit of health care systems and patients.

RESUMEN Objetivo: Determinar los tiempos quirúrgicos estándar de los cuatro procedimientos más comunes en cirugía general (hernioplastia inguinal unilateral, hernioplastia inguinal bilateral, hernioplastia umbilical y colecistectomía) de un hospital de segundo nivel y calcular la probabilidad de extensión de cada uno de los procedimientos. La eficiencia es un fenómeno ampliamente estudiado en el ámbito económico, pues hace referencia a la necesidad de menor cantidad de factores para la producción de un determinado nivel de bienes y servicios, por ello, es de vital importancia incluirlo en el ámbito quirúrgico. Materiales y métodos: Estudio observacional, descriptivo y retrospectivo. Se utilizaron los registros de quirófano de un hospital de segundo nivel del año 2017 al 2019 del servicio de Cirugía General. A partir de esta información, se estandarizó el tiempo necesario para cada procedimiento mediante la media de cada uno (hernioplastia umbilical, hernioplastia inguinal unilateral o bilateral y colecistectomía). Se calculó la probabilidad de extensión de las cirugías tomando en consideración los datos obtenidos y el intervalo de confianza. Resultados: Para el procedimiento de hernioplastia inguinal unilateral se obtuvo una media de 76 min (IC 95,00 %: 72-80 min, DE 23); en hernioplastia inguinal bilateral, una media de 104,38 min (IC 95,00 %: 91-116 min, DE 41,7); en hernioplastia umbilical, una media de 59,31 min (IC 95,00 %: 54-63 min, DE 29,9), y en colecistectomía, una media de 85,735 min (IC 95,00 %: 83-88 min). La probabilidad de que se programen tres cirugías y todas estén a tiempo (límite superior del IC) es de 92,69 %, la probabilidad de que se programen tres cirugías y todas se prolonguen es de 0,0016 % (límite inferior del IC). Conclusiones: Es posible realizar la planeación de las cirugías programadas mediante el uso de tiempos quirúrgicos estandarizados. Se requiere contar con estadística actualizada de los procedimientos quirúrgicos (promedios del tiempo de realización de cada procedimiento), ya que es posible detectar y supervisar de manera más precisa la dinámica de quirófano mediante la detección de las áreas de oportunidad, de esta manera, se eficientizará el tiempo de quirófano para beneficio de los sistemas de salud y los pacientes.

ABSTRACT Objective. To estimate the costs incurred in the control of Aedes aegypti in the Loreto region, during the years 2017 and 2018. Materials and methods. We conducted a partial retrospective economic evaluation of the costs of Aedes aegypti control of the Regional Health Directorate Loreto, during the implementation of the Regional Plan for Surveillance and Control of Aedes aegypti. Documentation such as plans, intervention reports and payment slips were reviewed, and interviews were conducted with professional personnel involved in vector control, on the costs of control interventions. Results. We found that the costs incurred in dengue vector control in the Loreto Region in the two years were: PEN 3,807,858 and PEN 4,066,380 during 2017 and 2018, respectively (USD 1,175,264 and USD 1,1210,232 at the 2017 and 2018 exchange rate). However, the effect of control activities is short-lived. Conclusions. The high cost involved in vector control with the methods currently used and the short duration of its effect make it unsustainable. Studies should be conducted in order to find other more efficient methods for dengue control.

RESUMEN Objetivo. Estimar los costos incurridos en el control del Aedes aegypti en la región Loreto, en los años 2017 y 2018. Materiales y métodos. Se realizó una evaluación económica retrospectiva parcial de los costos del control del Aedes aegypti de la Dirección Regional de Salud Loreto, durante la ejecución del Plan Regional de Vigilancia y Control de Aedes aegypti. Se revisó documentación como planes, informes de intervenciones y planillas de pago y se realizaron entrevistas al personal profesional implicado en el control vectorial, sobre los costos de las intervenciones de control. Resultados. Se halló, que los costos incurridos en el control del vector del dengue en la Región Loreto en los dos años estudiados ascienden a: 3,807,858 PEN y 4,066,380 PEN durante el 2017 y 2018, respectivamente (1´175,264 USD y 1´1210,232 USD al tipo de cambio del 2017 y 2018). Sin embargo, el efecto de las actividades de control es de corta duración. Conclusiones. El alto costo que implica el control vectorial con los métodos usados actualmente y la corta duración de su efecto lo hace insostenible. Se deben realizar estudios para hallar otros métodos más eficientes para el control del dengue.

ABSTRACT Objective. To estimate the costs incurred in the control of Aedes aegypti in the Loreto region, during the years 2017 and 2018. Materials and methods. We conducted a partial retrospective economic evaluation of the costs of Aedes aegypti control of the Regional Health Directorate Loreto, during the implementation of the Regional Plan for Surveillance and Control of Aedes aegypti. Documentation such as plans, intervention reports and payment slips were reviewed, and interviews were conducted with professional personnel involved in vector control, on the costs of control interventions. Results. We found that the costs incurred in dengue vector control in the Loreto Region in the two years were: PEN 3,807,858 and PEN 4,066,380 during 2017 and 2018, respectively (USD 1,175,264 and USD 1,1210,232 at the 2017 and 2018 exchange rate). However, the effect of control activities is short-lived. Conclusions. The high cost involved in vector control with the methods currently used and the short duration of its effect make it unsustainable. Studies should be conducted in order to find other more efficient methods for dengue control.

RESUMEN Objetivo. Estimar los costos incurridos en el control del Aedes aegypti en la región Loreto, en los años 2017 y 2018. Materiales y métodos. Se realizó una evaluación económica retrospectiva parcial de los costos del control del Aedes aegypti de la Dirección Regional de Salud Loreto, durante la ejecución del Plan Regional de Vigilancia y Control de Aedes aegypti. Se revisó documentación como planes, informes de intervenciones y planillas de pago y se realizaron entrevistas al personal profesional implicado en el control vectorial, sobre los costos de las intervenciones de control. Resultados. Se halló, que los costos incurridos en el control del vector del dengue en la Región Loreto en los dos años estudiados ascienden a: 3,807,858 PEN y 4,066,380 PEN durante el 2017 y 2018, respectivamente (1´175,264 USD y 1´1210,232 USD al tipo de cambio del 2017 y 2018). Sin embargo, el efecto de las actividades de control es de corta duración. Conclusiones. El alto costo que implica el control vectorial con los métodos usados actualmente y la corta duración de su efecto lo hace insostenible. Se deben realizar estudios para hallar otros métodos más eficientes para el control del dengue.

Abstract OBJECTIVE: To describe the sociodemographic, clinical and pathological characteristics and results of the patent blue sentinel lymph node technique in early breast cancer surgery. And to report the experience in identifying the sentinel lymph node in early breast cancer using the 2.5% patent blue technique. MATERIALS AND METHODS: Retrospective and analytical study consisting of the evaluation of the clinical records of patients diagnosed with early breast cancer, without clinical or radiological suspicion of axillary involvement, seen between June 2022 and June 2023 at the Oncological Gynaecology Service of the UMAE Hospital de Ginecoobstetricia, Centro Médico Nacional de Occidente of the IMSS. The dye injection site was subdermal periareolar, and the identified lumps were examined in the transoperative period. The percentage of identification, false negative rates and negative predictive value of the method were analysed. RESULTS: Ninety-five sentinel node biopsies were analysed. Axillary lymphadenectomy was performed only in patients with sentinel lymph node metastasis confirmed at surgery and in those in whom no stained nodes were identified due to non-migration of the dye. The mean age of the patients was 57.1 years range 25 to 78 years. The tumour size was less than 3 cm. Sixty-four patients 67% underwent mastectomy, compared with 31 who underwent breast-conserving surgery 33%. Fifty-seven of the 95 patients were staged as AI; the most common molecular subtype was compatible with luminal A in 49%. CONCLUSIONS: Sentinel lymph node biopsy with patent blue is a rapid, simple, accurate and inexpensive technique for identifying axillary disease in early breast cancer. The results reported here represent an initial evaluation of the technique in our service.

Resumen OBJETIVO: Describir las características sociodemográficas, clínicas y patológicas y los resultados obtenidos con la técnica de ganglio centinela con azul patente en la cirugía de cáncer de mama temprano. Además, reportar la experiencia en la identificación del ganglio centinela en cáncer de mama temprano con la técnica con azul patente al 2.5%. MATERIALES Y MÉTODOS: Estudio retrospectivo y analítico consistente en la evaluación de los expedientes clínicos de pacientes diagnosticadas con cáncer de mama temprano, sin sospecha clínica o radiológica de afectación axilar, atendidas entre junio de 2022 y junio de 2023 en el servicio de Ginecología Oncológica de la UMAE Hospital de Ginecoobstetricia, Centro Médico Nacional de Occidente del IMSS. El sitio de inyección del colorante fue subdérmico periareolar, los ganglios identificados se estudiaron en el transoperatorio. Se analizaron el porcentaje de identificación, las tasas de falsos negativos y el valor predictivo negativo del método. RESULTADOS: Se analizaron 95 procedimientos de biopsia de ganglio centinela. Solo se practicó la linfadenectomía axilar en las pacientes con metástasis en el ganglio centinela comprobada en el estudio transoperatorio y en las que no se identificaron ganglios teñidos por no migración del colorante. La edad promedio de las pacientes fue de 57.1 años límites 25 y 78 años. El tamaño del tumor fue menor a 3 cm. A 64 67% pacientes se les hizo la mastectomía en comparación con 31 a quienes se efectuó cirugía conservadora de mama 33%. Se estadificaron como IA 57 de las 95 pacientes; el subtipo molecular más frecuente fue compatible con luminal A en 49%. CONCLUSIONES: La biopsia del ganglio centinela, con azul patente, es una técnica rápida, sencilla, precisa y de bajo costo para identificar daño axilar en etapas tempranas del cáncer de mama. Lo aquí reportado son resultados que corresponden a una primera evaluación de la técnica en nuestro servicio.

ABSTRACT Introduction: The Delphi method is used in medical sciences to predict an event by means of an intuitive judgment, issued by a group of experts with the help of questionnaires to achieve consensus. Objective: To demonstrate the usefulness of the Delphi method to define the applicability, feasibility, relevance, update, scientific level and the need for the introduction of a self-care strategy for older adults with colorectal cancer during the COVID-19 pandemic. Methods: A descriptive, prospective and cross-sectional research was conducted from January to March 2021. The Delphi method was applied in stages to obtain consensus. Consent was obtained from the experts to participate in the anonymous assessment and those with high competence were selected. The strategy proposal was given to them, which allowed them to understand better and make a value judgment with respect to it. Results: There was a predominance of family medicine specialists, graduated or specialized since more than 10 years ago, with a master’s in sciences degree or auxiliary professors. The selected 15 obtained a high competence coefficient. The category of very adequate predominated for applicability, feasibility, implementation, need of introduction, update and scientific level of the actions and educational activities contained in the strategy. Conclusions: The Delphi method is useful to determine the applicability, feasibility, relevance, update, scientific level and the need for introduction of the strategy.

RESUMEN Introducción: El Método Delphi es utilizado en las Ciencias Médicas para predecir un suceso mediante un juicio intuitivo, emitido por un grupo de expertos con la ayuda de cuestionarios para conseguir consenso. Objetivo: Demostrar la utilidad del Método Delphi para definir la aplicabilidad, factibilidad, pertinencia, actualidad, nivel científico y la necesidad de la introducción de la estrategia de autocuidado para adulto mayor con cáncer colorrectal durante la pandemia de COVID-19. Métodos: Se realizó una investigación descriptiva, prospectiva y transversal entre enero a marzo del 2021. Se aplicó el método Delphi por etapas para la obtención de consenso. Se obtuvo consentimiento de los expertos para participar en la valoración anónima y se seleccionaron los de competencia alta. Se entregó la propuesta de la estrategia, que les permitió una mejor comprensión y posibilidad de emitir un juicio de valor al respecto. Resultados: Predominaron los especialistas en Medicina General Integral, con más de 10 años de graduados y de especialista, máster en ciencia y profesores auxiliares. Los 15 seleccionados obtuvieron coeficiente de competencia alta. Predominó la categoría de muy adecuada a la aplicabilidad, factibilidad, implementación, necesidad de la introducción, actualidad y nivel científico de las acciones y actividades educativas contenidas en la estrategia. Conclusiones: El Método Delphi es útil para determinar la aplicabilidad, factibilidad, pertinencia, actualidad, nivel científico y la necesidad de la introducción de la estrategia.

Resumo O objetivo deste trabalho foi avaliar o efeito da adição dietética de anacardato de cálcio como fonte de ácido anacárdico sobre o desempenho reprodutivo e os parâmetros antioxidantes sanguíneos de coelhas Nova Zelândia Branco. Vinte e sete fêmeas da raça Nova Zelândia Branco foram distribuídas em delineamento em blocos ao acaso, com três tratamentos e nove repetições, tendo-se considerado um animal como unidade experimental. Os tratamentos consistiram em rações peletizadas, com diferentes níveis de inclusão de anacardato de cálcio (0, 0,5 e 1,0%). A inclusão de anacardato de cálcio nas dietas aumentou a concentração de compostos fenólicos e a capacidade antioxidante no sangue das coelhas fêmeas lactantes. O anacardato de cálcio ao nível de 1,0% melhora a conversão alimentar durante a gestação e aumenta os compostos fenólicos e a capacidade antioxidante no soro sanguíneo das coelhas aos 21 dias de lactação. acaso repetições tendose tendo se experimental peletizadas 0, 0 (0 05 5 1,0%. 10 . 1 1,0%) lactantes 1,0 2 lactação ( 1,

Abstract The objective of this work was to evaluate the effect of the dietary addition of calcium anacardate as a source of phenolic compounds on the reproductive performance and blood antioxidant parameters of New Zealand White female rabbits. Twenty-seven New Zealand White female rabbits were distributed in a randomized complete block design with three treatments and nine replicates, considering one animal as the experimental unit. The treatments consisted of pelleted diets with different inclusion levels of calcium anacardate (0, 0.5, and 1.0%). The inclusion of calcium anacardate in the diets increased the concentration of phenolic compounds and the antioxidant capacity in the blood of lactating female rabbits. Calcium anacardate at the level of 1.0% improves the feed conversion ratio during gestation and increases the phenolic compounds and antioxidant capacity in the blood serum of female rabbits at 21 days of lactation. Twentyseven Twenty seven replicates unit 0, 0 (0 05 5 0.5 1.0%. 10 . 1 1.0%) 1.0 2 lactation ( 0. 1.

Abstract Sesbania virgata (Cav.) Pers. seeds are protein sources with health and environmental benefits. In this research, proteins with lectin activity were identified in a protein fraction from S. virgata seeds (PFLA), as well its antioxidant and antimicrobial potentials, in addition to cytotoxic effects. To obtain PFLA, seed flour was homogenized in Glycine-NaOH (100 mM; pH 9.0; NaCl 150 mM) and precipitated in ammonium sulfate. PFLA concentrates bioactive lectins (32 HU/mL, 480 HU/gFa, 18.862 HU/mgP) and essential amino acids (13.36 g/100g protein). PFLA exerts antioxidant activity, acting as a promising metal chelating agent (~77% of activity). Analyzes of cell culture assay results suggest that antioxidant activity of PFLA may be associated with the recruitment of essential molecules to prevent the metabolic impairment of cells exposed to oxidative stress. PFLA (256 – 512 µg/mL) also exhibits antifungal activity, inhibiting the growth of Aspergillus flavus, Candida albicans, Candida tropicalis and Penicillium citrinum. Cytotoxic analysis indicates a tendency of low interference in the proliferation of 3T3 and HepG2 cells in the range of PFLA concentrations with biological activity. These findings support the notion that PFLA is a promising adjuvant to be applied in current policies on the management of metal ion chelation and fungal infections. Cav. Cav (Cav. Pers benefits research S , (PFLA) potentials effects GlycineNaOH Glycine NaOH 100 (10 mM 9.0 90 9 0 15 sulfate 32 (3 HUmL HU mL HU/mL 48 HUgFa gFa HU/gFa 18862 18 862 18.86 HU/mgP HUmgP mgP 13.36 1336 13 36 (13.3 g100g gg g 100g protein. . protein) ~77% 77 (~77 activity) stress 256 (25 51 µg/mL µgmL µg flavus albicans citrinum T 3T HepG infections (Cav (PFLA 10 (1 9. 1 3 ( 4 1886 86 18.8 13.3 133 (13. ~77 7 (~7 25 (2 5 188 8 18. 13. (13 ~7 (~ 2 ~

RESUMO Introdução: A incapacidade funcional causada por alterações físicas leva a limitações significativas nas atividades diárias e gera um grande impacto no retorno das pessoas com deficiência ao espaço social e ao local de trabalho, demandando a avaliação da influência em longo prazo do uso de um dispositivo especialmente desenvolvido para a postura ortostática nos parâmetros fisiológicos, biomecânicos e funcionais de pacientes amputados e com medula espinhal. Objetivo: O objetivo foi avaliar o efeito do uso do dispositivo de suporte postural na função, dor e parâmetros biomecânicos e cardiológicos em pacientes com lesão medular e amputados em comparação com um grupo controle. Métodos: O aparelho ortostático foi utilizado pelos participantes por um período de dez dias consecutivos, em três ciclos de 50 minutos diários, com acompanhamento de 15 dias. Os participantes foram posicionados e estabilizados por meio de alças ajustáveis nos ombros, tronco e quadris. O desfecho primário foi o questionário Breve Inventário de Dor. Quinze participantes foram incluídos no grupo controle, 15 no grupo amputado e 15 no grupo medular. Resultados: Nossos resultados demonstram que o uso do dispositivo permite a posição ortostática de amputados e pacientes com lesão medular avaliados por dez dias, levando a melhora da funcionalidade e dor nos grupos de amputados e medula espinhal em relação ao grupo controle. Além disso, não foram observadas alterações nos resultados secundários, indicando que o uso do dispositivo não causou interferência prejudicial aos pacientes. Conclusão: O uso prolongado do dispositivo ortostático é benéfico para melhorar a funcionalidade, reduzir a dor em amputados e pacientes com lesão medular. Nível de Evidência II; Estudos Terapêuticos - Investigação dos resultados de tratamento. Introdução trabalho fisiológicos Objetivo função controle Métodos consecutivos 5 diários 1 ombros quadris Dor Resultados disso secundários Conclusão II tratamento

ABSTRACT Introduction: Functional incapacity caused by physical alterations leads to significant limitations in daily activities and has a major impact on the return of people with disabilities to the social space and the workplace. This calls for an evaluation of the long-term influence of the use of a device specially developed for orthostatic posture on the physiological, biomechanical and functional parameters of amputees and spinal cord patients. Objective: The objective was evaluate the effect of postural support device use on function, pain, and biomechanical and cardiologic parameters in spinal cord injury and amputees patients compared to a control group. Methods: The orthostatic device was used by the participants for a period of ten consecutive days, for three cycles of 50 minutes each day, and a 15-day follow-up. Participants were positioned and stabilized using adjustable straps on the shoulders, trunk, and hips. The primary outcome was brief pain inventory. Fifteen participants were included the control group, 15 in the amputee group, and 15 in the spinal cord group. Results: Our results demonstrate that the use of the device allows the orthostatic position of amputees and spinal cord patients evaluated for ten days, leading to improved functionality and pain in the spinal cord and amputee groups compared to the control group. In addition, no changes were observed for secondary outcomes, indicating that the use of the device did not cause harm interference to patients. Conclusion: The long-term use of the orthostatic device is beneficial for improving functionality, reduce pain in amputees and spinal cord injury patients. Level of evidence II; Therapeutic Studies - Investigating the results of treatment. Introduction workplace longterm long term physiological Objective function group Methods days 5 day 15day followup. followup follow up. up follow-up shoulders trunk hips inventory 1 Results addition outcomes Conclusion II treatment

ABSTRACT Introduction: Every worker dedicated to patient care must constantly update the profile to which they are linked. In the case of the Provincial Psychiatric Teaching Hospital of Sancti Spiritus, health personnel should receive some undergraduate or postgraduate training on the management of patients with serious mental illness. In this institution, a comprehensive rehabilitation program is implemented for the hospitalized adult with schizophrenia. Objective: To evaluate the results of the training of mental health teams on the rehabilitation of hospitalized schizophrenic patients. Methods: An interventional study was carried out between February 4 and May 6, 2019, which included 19 professionals. The variable considered was “level of information,” measured on a scale of three categories: high, medium and low. To evaluate the quality of the applied survey, procedures were applied to quantify validity and reliability. Results: The training course was designed with 160 hours for participating health personnel. The level of information was medium before receiving the course, and once taught it was high. Conclusions: The training carried out could have influenced the increase in the level of information of the mental health teams, related to the rehabilitation of adult schizophrenic patients of the psychiatric hospital. Despite the limitation of having been developed in a single hospital and with a small participating population, the authors consider that it could be successfully applied in institutions with similar characteristics.

RESUMEN Introducción: Todo trabajador dedicado a la atención a pacientes debe mantener una constante actualización sobre el perfil al cual se vincula. En el caso del Hospital Psiquiátrico Docente Provincial de Sancti Spíritus, el personal de salud debería recibir alguna capacitación en pre o posgrado sobre el manejo de pacientes con enfermedades mentales graves. En dicha institución se implementa un programa de rehabilitación integral para el adulto con esquizofrenia hospitalizado. Objetivo: Evaluar los resultados de la capacitación a los equipos de salud mental sobre la rehabilitación de los pacientes esquizofrénicos hospitalizados. Métodos: Se realizó un estudio de intervención, entre el 4 de febrero y el 6 de mayo de 2019, que incluyó a 19 profesionales. La variable considerada fue “nivel de información”, medida en una escala de tres categorías: alto, medio y bajo. Para evaluar la calidad de la encuesta aplicada, se aplicaron procedimientos para cuantificar la validez y la confiabilidad. Resultados: Se diseñó el curso de capacitación con 160 horas para el personal de salud participante. El nivel de información presentó categoría medio antes de recibir el curso, y una vez impartido, fue alto. Conclusiones: La capacitación realizada pudiera haber influido en el aumento del nivel de información de los equipos de salud mental, relacionado con la rehabilitación de pacientes adultos esquizofrénicos del hospital psiquiátrico. A pesar de tener como limitante el haberse desarrollado en un solo hospital y con poca población participante, los autores consideran que podría aplicarse con éxito en instituciones con características similares.

RESUMO Objetivo: Verificar e comparar a radiopacidade de 04 resinas compostas do mercado nacional, e analisar se estão no padrão Organização Internacional de Normalização 4049 de radiopacidade. Métodos: Foram confeccionados 05 corpos de prova para cada resina composta Bulk Fill testada , que foram radiografados ao lado de uma cunha de degrau de alumínio com um sistema de imagem digital. As imagens foram submetidas a um software capaz medir a valores médios de cinza (MGVs), os quais foram convertidos em milímetros de alumínio. Os dados foram analisados estatisticamente (p0,05). Resultados: As 04 resinas compostas testadas mostraram valores de radiopacidade notadamente maiores que o equivalente ao esmalte e dentina, destacando-se a Tetric N-Ceram- Ivoclar Vivadent (11,05 mmAl). Todas estão dentro do padrão ISO 4049 de radiopacidade. Ao compará-las entre si, houve diferença estatisticamente significativa entre os grupos de resinas Opus Bulk Fill e Tetric N-Ceram (p-valor 0,05). Conclusão: Todos os materiais mostraram radiopacidade superior ao equivalente a dentina e esmaltes. Todas estão no padrão Organização Internacional de Normalização 4049. Mais pesquisas devem ser realizadas devido ao reduzido número de estudos realizados com as resinas compostas em questão. Objetivo 0 nacional 404 Métodos digital MGVs, MGVs (MGVs) p0,05. p005 p p0,05 . p0 (p0,05) Resultados destacandose destacando NCeram N Ceram 11,05 1105 11 (11,0 mmAl. mmAl mmAl) comparálas compará las si pvalor valor 0,05. 005 0,05 0,05) Conclusão esmaltes questão 40 (MGVs p00 p0,0 (p0,05 11,0 110 1 (11, 00 0,0 4 p0, (p0,0 11, (11 0, (p0, (1 (p0 ( (p

ABSTRACT Objective: To verify and compare the radiopacity of four composite resins available in the national market and analyze their compliance with International Standards Organization 4049 guidelines. Methods: Five specimens of each Bulk Fill composite resin were prepared, and radiographs were taken alongside an aluminum step wedge using a digital imaging system. The images were processed using software capable of measuring mean grey values (MGVs), which were then converted to millimeters of aluminum. Statistical analysis was performed (P 0.05). Results: The four tested composite resins showed radiopacity values higher than those of enamel and dentin, standing out the Tetric N-Ceram- Ivoclar Vivadent (11.05 mmAl). All samples are in accord with the ISO 4049 radiopacity standard. The resin Opus Bulk Fill and Tetric N-Ceram were compared to each other, revealing statistically significant differences (p0,05). Conclusion: All materials demonstrated radiopacity values higher than those of dentin and enamel. All samples are in accordance with the International Organization for Standardization 4049 guidelines. More studies should be conducted with the composite resins used in this study due to the limited research involving these specific resins. Objective 404 guidelines Methods prepared system MGVs, MGVs , (MGVs) P 0.05. 005 0.05 . 0 05 0.05) Results NCeram N Ceram 11.05 1105 11 (11.0 mmAl. mmAl mmAl) standard other p0,05. p005 p p0,05 p0 (p0,05) Conclusion 40 (MGVs 00 0.0 11.0 110 1 (11. p00 p0,0 (p0,05 4 0. 11. (11 p0, (p0,0 (1 (p0, ( (p0 (p

Abstract The polymer transition temperature is a crucial parameter in the industry for knowing raw materials before starting the manufacturing process. The current work reports a novel low-cost prototype instrument to measure the transition temperature with reliable accuracy. The equipment was built using commercial load cells composed of strain gauges in combination with an Arduino® microcontroller. The prototype measurement quality was validated by measuring the transition temperatures of most commercial polymers. The obtained values were compared with values obtained by conventional thermal analysis known as differential scanning calorimetric and thermo-mechanical analysis (DSC and TMA), which results in identical values. process lowcost low cost accuracy Arduino microcontroller polymers thermomechanical thermo mechanical DSC TMA, TMA , TMA)