ABSTRACT Background: Here, Leishmania presence in sand flies from Três Lagoas, Mato Grosso do Sul, Brazil, after visceral leishmaniasis (VL) was investigated. Methods: In April 2022, two light traps were deployed within and around the residence for two days post-VL case report. Results: A total of 120 Lutzomyia longipalpis were collected. Suprapyloric flagellates were found in a female sand fly with eggs and residual blood during midgut dissection. Sequencing of ITS1 and cytb fragments confirmed Leishmania infantum DNA and identified Homo sapiens as the blood source, respectively. Conclusions: This study emphasizes the importance of monitoring sand flies in VL endemic areas. Background Here Lagoas Sul Brazil (VL investigated Methods 2022 postVL post report Results 12 collected dissection ITS source respectively Conclusions areas 202 1 20 2

Sandflies associated with opossum nests are reported for the first time in the yards of residences located in the urban area of the municipality of Monte Mor, situated in the metropolitan region of Campinas, state of São Paulo, Brazil. Eleven specimens of Evandromyia cortelezzii and one of Evandromyia lenti were captured in two Didelphis albiventris nests. Ev. cortelezzii is considered a secondary vector species for the transmission of Leishmania (Viannia) braziliensis and Leishmania (Leishmania) infantum in the Neotropics. This association may contribute to the introduction, establishment and maintenance of urban and periurban zoonotic transmission outbreaks of Leishmania and should therefore be investigated further.

This study was conducted to identify the sandfly fauna and the anthropophilic species in a coffee-growing area of Villanueva, Norte de Santander, Colombia, a focus of American cutaneous leishmaniasis, and to analyse the relationship between the most frequent species and rainfall, relative humidity and temperature, with the aim of contributing to epidemiological surveillance in the area. Sandfly collections were performed fortnightly between February 2006-September 2007 using automatic light traps, Shannon traps, protected human bait and aspiration in resting places. A total of 7,051 sandflies belonging to 12 species were captured. Pintomyia spinicrassa (95.7%) predominated. Pintomyia oresbia and Lutzomyia sp. of Pichinde were found in the state of Norte de Santander for the first time. Pi. spinicrassa, Pintomyia nuneztovari, Micropygomyia venezuelensis, Lutzomyia (Helcocyrtomyia) scorzai and Lu. (Helcocyrtomyia) sp. were captured on the protected human bait. A significant association between Pi. spinicrassa abundance and the total rainfall and the average temperature and humidity 10 days before the collection was observed. The dominance of Pi. spinicrassa, a recognised vector of Leishmania braziliensis, especially during the dry periods, indicates that the risk of parasite transmission may increase.

Casos humanos de leishmaniose tegumentar americana (LTA) têm sido registrados na Serra da Cantareira, região da Grande São Paulo, onde se situam o Parque Estadual da Cantareira e o Parque Estadual Alberto Löfgren. O estudo teve como objetivo identificar a fauna flebotomínea e alguns de seus aspectos ecológicos nos dois parques e área adjacente, para investigar vetores de Leishmania sp. As capturas ocorreram de janeiro a dezembro de 2009, das 18 às 06 horas, com armadilhas automáticas luminosas instaladas em matas e peridomicílios, e com armadilhas de Shannon modificadas, nas cores branca e preta, em peridomicílio. Foram capturadas 12 espécies e 5.436 flebotomíneos por armadilhas automáticas luminosas (141), armadilhas de Shannon (5.219) e tentando picar os pesquisadores enquanto coletavam na armadilha de Shannon (76). Pintomyia fischeri e Migonemyia migonei foram as mais abundantes. Pi. fischeri predominou nos três métodos de coleta com 49,0%, 88,8% e 65,8%, respectivamente, e Mg. migonei foi a segunda mais prevalente na Shannon (10,0%) e tentando picar os pesquisadores (22,4%). Fêmeas de Pi. fischeri foram significativamente mais atraídas à Shannon preta e Mg. migonei à branca. Houve correlação positiva e significante entre o número de Pi. fischeri e a média das mínimas da umidade relativa nos 15 dias anteriores ao dia da coleta, e negativa e significante para as duas espécies mais abundantes em relação a umidade relativa no dia da coleta. Altas frequências e antropofilia de Pi. fischeri e de Mg. migonei sugerem que ambas podem estar atuando na transmissão da LTA na área.

Human cases of American cutaneous leishmaniasis (ACL) have been recorded in Serra da Cantareira, in the Greater São Paulo Metropolitan Region, where two conservation units are situated, the Parque Estadual da Cantareira and the Parque Estadual Alberto Löfgren. The present study aimed to identify the sandfly fauna and some of its ecological aspects in these two parks and their surrounding area to investigate Leishmania sp. vectors. The captures were undertaken monthly from January to December 2009, from 6:00 p.m. to 6:00 a.m., with automatic light traps installed in forests and peridomicile areas and with modified black/white Shannon traps in the peridomicile. A total of 12 species and 5,436 sandflies were captured: with automatic light traps (141), Shannon traps (5,219) and attempting to bite the researchers while they were conducting the collection in Shannon traps (76). Pintomyia fischeri and Migonemyia migonei were the most abundant species. Pi. fischeri predominated in all three kinds of captures (49%, 88.8% and 65.8%, respectively). Mg. migonei was the second most prevalent in Shannon traps (10.0%) and attempting to bite the researchers (22.4%). Pi. fischeri females were significantly more attracted to black and those of Mg. migonei to white Shannon traps. A positive and significant correlation was observed between the numbers of Pi. fischeri and the mean of minimum relative humidity values on the fifteen days prior to capture, while there was a negative and significant correlation between the relative humidity on the capture day and the two most abundant species. The anthropophilia and high frequencies of Pi. fischeri and Mg. migonei suggest that both species may be transmitting ACL agents in this region.

Casos humanos de leishmaniose tegumentar americana (LTA) têm sido registrados na Serra da Cantareira, região da Grande São Paulo, onde se situam o Parque Estadual da Cantareira e o Parque Estadual Alberto Löfgren. O estudo teve como objetivo identificar a fauna flebotomínea e alguns de seus aspectos ecológicos nos dois parques e área adjacente, para investigar vetores de Leishmania sp. As capturas ocorreram de janeiro a dezembro de 2009, das 18 às 06 horas, com armadilhas automáticas luminosas instaladas em matas e peridomicílios, e com armadilhas de Shannon modificadas, nas cores branca e preta, em peridomicílio. Foram capturadas 12 espécies e 5.436 flebotomíneos por armadilhas automáticas luminosas (141), armadilhas de Shannon (5.219) e tentando picar os pesquisadores enquanto coletavam na armadilha de Shannon (76). Pintomyia fischeri e Migonemyia migonei foram as mais abundantes. Pi. fischeri predominou nos três métodos de coleta com 49,0%, 88,8% e 65,8%, respectivamente, e Mg. migonei foi a segunda mais prevalente na Shannon (10,0%) e tentando picar os pesquisadores (22,4%). Fêmeas de Pi. fischeri foram significativamente mais atraídas à Shannon preta e Mg. migonei à branca. Houve correlação positiva e significante entre o número de Pi. fischeri e a média das mínimas da umidade relativa nos 15 dias anteriores ao dia da coleta, e negativa e significante para as duas espécies mais abundantes em relação a umidade relativa no dia da coleta. Altas frequências e antropofilia de Pi. fischeri e de Mg. migonei sugerem que ambas podem estar atuando na transmissão da LTA na área.

Human cases of American cutaneous leishmaniasis (ACL) have been recorded in Serra da Cantareira, in the Greater São Paulo Metropolitan Region, where two conservation units are situated, the Parque Estadual da Cantareira and the Parque Estadual Alberto Löfgren. The present study aimed to identify the sandfly fauna and some of its ecological aspects in these two parks and their surrounding area to investigate Leishmania sp. vectors. The captures were undertaken monthly from January to December 2009, from 6:00 p.m. to 6:00 a.m., with automatic light traps installed in forests and peridomicile areas and with modified black/white Shannon traps in the peridomicile. A total of 12 species and 5,436 sandflies were captured: with automatic light traps (141), Shannon traps (5,219) and attempting to bite the researchers while they were conducting the collection in Shannon traps (76). Pintomyia fischeri and Migonemyia migonei were the most abundant species. Pi. fischeri predominated in all three kinds of captures (49%, 88.8% and 65.8%, respectively). Mg. migonei was the second most prevalent in Shannon traps (10.0%) and attempting to bite the researchers (22.4%). Pi. fischeri females were significantly more attracted to black and those of Mg. migonei to white Shannon traps. A positive and significant correlation was observed between the numbers of Pi. fischeri and the mean of minimum relative humidity values on the fifteen days prior to capture, while there was a negative and significant correlation between the relative humidity on the capture day and the two most abundant species. The anthropophilia and high frequencies of Pi. fischeri and Mg. migonei suggest that both species may be transmitting ACL agents in this region.