RESUMO: Este estudo descreve os aspectos patológicos de um carcinoma mamário simples túbulo-papilar metastático grau II em um lobo-guará, fêmea, senil de vida livre. O diagnóstico foi baseado nos achados histopatológicos de células malignas epiteliais arranjadas em um padrão tubular e papilar, com acentuada anisocitose e anisocariose e metástases para os linfonodos regionais, pulmão, pleura parietal e pericárdio. Também associado a marcação imuno-histoquímica positiva das células neoplásicas para Citoqueratina AE1/AE3 e negativas para P63, Calponina e Cox-2, somado ao índice de proliferação celular de 26,8%. Os achados demonstram que canídeos silvestres de vida livre podem desenvolver neoplasias mamárias similares às observadas em canídeos domésticos. RESUMO túbulopapilar túbulo papilar loboguará, loboguará lobo guará, guará lobo-guará fêmea regionais pulmão pericárdio imunohistoquímica imuno histoquímica AE1AE3 AEAE AE1 AE3 AE AE1/AE P63 P Cox2, Cox2 Cox 2, 2 Cox-2 268 26 8 26,8% domésticos AE1AE P6 Cox- 26,8 26,

ABSTRACT: This study described the pathological aspects of a metastatic grade II simple tubulopapillary mammary carcinoma in a free-living senile female maned wolf (Chrysocyon brachyurus). The diagnosis was based on histopathological findings of malignant epithelial cells arranged in tubular and papillary patterns, with marked anisocytosis and anisokaryosis and metastases to regional lymph nodes, lungs, parietal pleura, and pericardium. Also associated to positive immunolabeling of neoplastic cells for Cytokeratin AE1/AE3 and negative for P63, Calponin and Cox-2, added to the cell proliferation index of 26.8%. The findings demonstrated that free-ranging wild canids develop mammary neoplasms similar to those observed in domestic canids. ABSTRACT freeliving free living Chrysocyon brachyurus. brachyurus . brachyurus) patterns nodes lungs pleura pericardium AE1AE3 AEAE AE1 AE3 AE AE1/AE P63 P Cox2, Cox2 Cox 2, 2 Cox-2 268 26 8 26.8% freeranging ranging AE1AE P6 Cox- 26.8 26.

Resumo Toxoplasma gondii e Neospora caninum são dois protozoários intimamente relacionados que infectam uma ampla gama de animais, incluindo-se aves. Entretanto, a ocorrência de N. caninum e T. gondii em aves marinhas é desconhecida. Portanto, este estudo objetivou determinar a presença de DNA de T. gondii e N. caninum em amostras de tecidos, coletadas de 47 aves marinhas. Amostras de tecidos dos músculos peitorais, coração e cérebro foram coletadas de 47 aves marinhas mortas ao longo da costa do estado de Santa Catarina, SC, Brasil. O DNA foi extraído dos tecidos e testado, usando-se a reação em cadeia da polimerase nested (nPCR), tendo em vista o espaçador interno transcrito 1 (ITS1). O DNA de T. gondii foi detectado em tecidos de sete aves marinhas (7/47, 14,8%), como gaivota (Larus dominicanus) (5/21) e bobo-pequeno (Puffinus puffinus) (2/8). O DNA de N. caninum foi detectado em tecidos de nove aves marinhas (9/47, 19,1%); gaivota (L. dominicanus) (4/21), bobo-pequeno (P. puffinus) (2/8), corvo-marinho (Phalacrocorax brasilianus) 1/4), atobá-pardo (Sula leucogaster) (1/5), e pardela-preta (Procellaria aequinoctialis) (1/1). No entanto, não foi observada coinfecção. Em conclusão, este estudo demonstrou a circulação de N. caninum e T. gondii em aves marinhas ao longo da costa de Santa Catarina, Brasil. Mais estudos são necessários para esclarecer o papel dessas aves na epidemiologia da neosporose e da toxoplasmose. animais incluindose incluindo se Entretanto N T desconhecida Portanto 4 peitorais Catarina SC Brasil testado usandose usando nPCR, nPCR , (nPCR) ITS1. ITS1 ITS . (ITS1) 7/47, 747 7 (7/47 14,8%, 148 14,8% 14 8 14,8%) Larus dominicanus 5/21 521 5 21 (5/21 bobopequeno bobo pequeno Puffinus puffinus 2/8. 28 2/8 2 (2/8) 9/47, 947 9 (9/47 19,1% 191 19 19,1%) L. L (L 4/21, 421 4/21 (4/21) P. P (P 2/8, corvomarinho corvo marinho Phalacrocorax brasilianus 1/4, 1/4 1/4) atobápardo atobá pardo Sula leucogaster 1/5, 15 1/5 (1/5) pardelapreta pardela preta Procellaria aequinoctialis 1/1. 11 1/1 (1/1) entanto coinfecção conclusão toxoplasmose (nPCR (ITS1 7/47 74 (7/4 14,8 5/2 52 (5/2 2/ (2/8 9/47 94 (9/4 19,1 42 4/2 (4/21 1/ (1/5 (1/1 (ITS 7/4 (7/ 14, 5/ (5/ (2/ 9/4 (9/ 19, 4/ (4/2 (1/ 7/ (7 (5 (2 9/ (9 (4/ (1 ( (4

Abstract Toxoplasma gondii and Neospora caninum are two closely related protozoans that infect a wide range of animals, including birds. However, the occurrence of N. caninum and T. gondii in seabirds is unknown. Therefore, this study aimed to determine the presence of T. gondii and N. caninum DNA in tissue samples of seabirds. Tissue samples of the pectoral muscles, heart, and brain were collected from 47 birds along the coastline of Santa Catarina State, SC, Brazil. The DNA was extracted from the tissues and screened using nested-PCR (nPCR) targeting internal transcribed spacer 1 (ITS1). T. gondii DNA was detected in tissues from seven seabirds (7/47, 14.8%), kelp gull (Larus dominicanus) (5/21), and Manx shearwater (Puffinus puffinus) (2/8). N. caninum DNA was detected in tissues of nine seabirds (9/47, 19.1%), the kelp gull (L. dominicanus) (4/21), Manx shearwater (P. puffinus) (2/8), neotropic cormorant (Phalacrocorax brasilianus) (1/4), brown booby (Sula leucogaster) (1/5), and white-chinned petrel (Procellaria aequinoctialis) (1/1); however, no co-infection was observed. In conclusion, this study showed the circulation of N. caninum and T. gondii in seabirds along the coastline of Santa Catarina State. Further studies are required to clarify the role of these birds in the epidemiology of neosporosis and toxoplasmosis. animals However N T unknown Therefore muscles heart 4 State SC Brazil nestedPCR nested PCR nPCR (nPCR ITS1. ITS1 ITS . (ITS1) 7/47, 747 7 (7/47 14.8%, 148 14.8% , 14 8 14.8%) Larus dominicanus 5/21, 521 5/21 5 21 (5/21) Puffinus puffinus 2/8. 28 2/8 2 (2/8) 9/47, 947 9 (9/47 19.1%, 191 19.1% 19 19.1%) L. L (L 4/21, 421 4/21 (4/21) P. P (P 2/8, Phalacrocorax brasilianus 1/4, 1/4 (1/4) Sula leucogaster 1/5, 15 1/5 (1/5) whitechinned white chinned Procellaria aequinoctialis 1/1 11 (1/1) however coinfection co infection observed conclusion toxoplasmosis (ITS1 7/47 74 (7/4 14.8 52 5/2 (5/21 2/ (2/8 9/47 94 (9/4 19.1 42 4/2 (4/21 1/ (1/4 (1/5 (1/1 (ITS 7/4 (7/ 14. 5/ (5/2 (2/ 9/4 (9/ 19. 4/ (4/2 (1/ 7/ (7 (5/ (2 9/ (9 (4/ (1 ( (5 (4

ABSTRACT This study evaluated how nutritional insults applied at different stages of intrauterine development affect the growth and development of total tract organs and the mRNA expression of genes that encode growth factors, tight junction proteins, digestive enzymes, and glucose transporters in the small intestine of newborn goats. Fourteen nulliparous dairy goats were assigned to one of two nutritional plans over gestation: maintenance-restriction (M-R, n = 8) – 100% of maintenance requirement from d 8-84 of gestation followed by feeding at 50% maintenance requirement from d 85 of gestation to parturition; or restriction-maintenance (R-M, n = 6) – 50% of maintenance requirement from d 8-84 of gestation followed by feeding at 100% maintenance requirement from d 85 of gestation to parturition. Fisher's least significant difference test was performed using SAS 9.4, considering a full fixed-effect model. When expressed per kg of body weight, the weights of the small intestine and total intestine were 18.7 and 18.1% lower, respectively, for R-M offspring. The lengths of the small intestine and the total intestine of the R-M newborns were 19.1 and 9.5% shorter, respectively. This group also had a lower villus height:intestinal crypt depth ratio. The R-M offspring tended to have lower Solute carrier family 5 member 1 (SLC5A1), Solute carrier family 2 member 2 (SLC2A2), and Occludin (OCLN) mRNA expressions. Therefore, feed restriction in the first half of gestation is detrimental to the second half of gestation, reducing the offspring's intestinal absorptive area but enhancing their intestinal permeability. factors proteins enzymes maintenancerestriction MR, MR M R, R (M-R 8 100 884 84 8-8 50 parturition restrictionmaintenance RM, RM M, (R-M 6 Fishers Fisher s 94 9 4 9.4 fixedeffect fixed effect model weight 187 18 7 18. 181 18.1 respectively 191 19 19. 95 9.5 shorter heightintestinal height ratio SLC5A1, SLC5A1 SLCA , SLC A (SLC5A1) SLC2A2, SLC2A2 (SLC2A2) OCLN (OCLN expressions Therefore offsprings permeability 10 88 8- 9. SLC5A (SLC5A1 SLC2A (SLC2A2 (SLC5A (SLC2A