ABSTRACT Background: The riverine communities of the Amazon comprise different social groups that inhabit the rural areas on the banks of rivers and lakes. Residents usually travel by river to rural and urban areas and are then exposed to urbanized diseases such as those caused by arbovirus infection. In Brazil, emerging diseases such as dengue, Zika, chikungunya, and those caused by infection with Oropouche and Mayaro viruses necessitate epidemiological surveillance. This study was aimed at determining the frequency of positivity for immunoglobulin (Ig)G and IgM antibodies against Zika, chikungunya, and dengue viruses and performing molecular analyses to detect viral RNA for the Zika, chikungunya, dengue virus, Oropouche, and Mayaro viruses, in the same serum samples obtained from riverside populations. Methods: This cross-sectional study was conducted in a riverside population in the Humaitá municipality of the Brazilian Amazon. More than 80% of the local population participated in this study. Entomological samples were collected to identify local mosquito vectors. Results: Analysis of 205 human serological samples revealed IgG antibodies against the dengue virus in 85 individuals. No molecular positivity was observed in human samples. Entomological analyses revealed 3,187 Diptera species, with Mansonia being the most frequent genus. Aedes aegypti and Aedes albopictus were not detected in the two collections. Conclusions: IgG antibodies against the dengue virus were highly prevalent, suggesting previous exposure. The absence of the arbovirus vectors Aedes aegypti and Aedes albopictus in the samples supports the hypothesis that the infections recorded likely occurred outside the riverside communities investigated. Background lakes Brazil Zika chikungunya surveillance Ig G populations Methods crosssectional cross sectional 80 Results 20 8 individuals 3187 3 187 3,18 species genus collections Conclusions prevalent exposure investigated 2 318 18 3,1 31 1 3,

ABSTRACT The study evaluates the influence of environmental enrichment on behavior, leucometry, and performance of commercial pigs. Thirty-two hybrid pigs (Landrace × Large White × Pietran), at 69 days of life, were divided into four groups and subjected to different environmental stimuli: concrete floor, with no enrichment (C); floor bedding with wood chips (CM); concrete floor, with mobiles (MO); floor bedding with wood chips and the presence of mobile (CM + MO). The study collected behavioral data of the animals during 84 days, through the growing and finishing phases, each lasting 42 days, assessing the animals’ position and behavior by the instantaneous scan sampling method. The animals received ad libitum water and a specially formulated feed following the nutritional requirements for growing and finishing phases. The study considered daily feed intake, daily weight gain, and feed conversion. Group CM in the growing phase showed prolonged standing position periods and demonstrated a greater exploration of their environment (2.1%). Groups MO and CM + MO, in the growing phase, demonstrated extended periods of interaction with the offered enrichments (10.8±2.1 and 9.1±2.8%, respectively). Piglets in the finishing phase housed in pens with floor bedding with wood chips and those housed in pens with floor bedding with wood chips and the presence of mobile (tires and chains) showed longer interaction time with enrichments (4.8 and 5.4%, respectively), compared with the other groups C and Mo. Group C remained overall the longest in a standing position in both breeding phases. There was no effect on the animals’ leukogram. Environmental enrichment with wood shavings (as beddings) and hanging mobiles improves behavioral aspects of piglets in the growing and finishing periods. leucometry Thirtytwo Thirty two Landrace Pietran, Pietran , Pietran) 6 life stimuli (C) (CM) (MO) MO. . MO) 8 phases 4 method intake gain conversion 2.1%. 21 2.1% 2 1 (2.1%) 10.8±2.1 10821 10 (10.8±2. 9128 9 9.1±2.8% respectively. respectively respectively) tires chains 4.8 48 (4. 54 5 5.4% respectively, Mo leukogram as beddings (C (MO 2.1 (2.1% 10.8±2. 1082 (10.8±2 912 9.1±2.8 4. (4 5.4 2. (2.1 10.8±2 108 (10.8± 91 9.1±2. ( 5. (2. 10.8± (10.8 9.1±2 (2 10.8 (10. 9.1± 10. (10 9.1 (1 9.

Abstract This study assessed the effect of different concentrations of (Capsicum baccatum var. pendulum) on lipid oxidation, microbiological and sensorial characteristics of four different formulations of fresh pork sausage and smoked (control, F1, F2, F3), with the substitution of industrial antioxidant for the natural one. Lipid oxidation values (TBARS) did not display significant differences (P>0.05) among the treatments, and at different times of analysis (storage). The only significant differences were between the different types of sausages (fresh and smoked). The enumeration of all the microorganisms searched was below the standard required by Brazilian laws. F2 formula received the highest scores for acceptance but did not display significant difference (P>0.05) as it refers to taste, with the remaining formulations. The substitution of the synthetic antioxidant by the natural one displayed similar results, demonstrating that the natural extract from the balloon pepper does not affect the technological and sensorial properties of the sausages, which proves the viability of the product.

RESUMO Objetivou-se com esse trabalho avaliar o uso de programas de luz sobre o desempenho, concentração plasmática de melatonina e comportamento de suínos em fase de terminação. O experimento foi conduzido no setor de suinocultura da UFLA, utilizando-se 32 suínos em fase de terminação, por 48 dias. Os animais foram alojados em grupos de dois em cada baia (unidade experimental), distribuídos em delineamento em blocos casualizados, de acordo com o peso inicial com quatro tratamentos e quatro blocos. Foram utilizados quatro programas de luz (tratamentos), sendo T1: luz natural (LN); T2: 15 horas de luz e nove de escuro por dia (15L: 9E); T3: 19 horas de luz por dia (19L: 5E); T4: 23 horas de luz por dia (23L: 1E). As variáveis analisadas foram o desempenho (consumo de ração médio diário, ganho de peso médio diário e conversão alimentar), concentração plasmática de melatonina e o comportamento (bebendo, fuçando, ócio e comendo). A alteração no fotoperíodo não alterou o desempenho ou a concentração plasmática de melatonina nos suínos. As variáveis comportamentais “fuçando”, “comendo”, “bebendo”, não diferiram em nenhum dos períodos estudados, porém o comportamento ócio foi mais ocorrente nos suínos que receberam 15 horas de luz. O aumento no fotoperíodo não permitiu expressivas alterações de desempenho ou de comportamento dos animais.

SUMMARY The aim of this study was to evaluate the use of lighting programs on performance, plasma concentration of melatonin and behavior of finishing pigs. The experiment was conducted in the pig farming sector UFLA, using 32 pigs in the finishing phase, for 48 days. The animals were housed in groups of two in each pen (experimental unit), distributed in a randomized block design, according to the initial weight. Four light periods were used (treatments): T1: natural light (LN); T2: 15 hours of light and dark by day nine (15L: 9E); T3: 19 hours light per day (19L: 5E); T4: 23 hours of light per day (23L: 1D). The variables analyzed were the performance (average daily feed consumption, average daily gain weight and feed conversion), the plasma concentrations of melatonin and the behavior (drinking, sniffing, leisure and eating). The change in photoperiod did not affect performance or melatonin concentration in pigs. The behavioral variables "digging", "eating", "drinking", did not differ in any of the periods studied, but leisure behavior was more occurring to the pigs receiving 15 hours of light. The increase in photoperiod showed no significant changes in performance or behavior of the animals.