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Abstract Background Patients with immune-mediated rheumatic diseases (IMRDs) have been prioritized for COVID-19 vaccination to mitigate the infection severity risks. Patients with rheumatoid arthritis (RA) are at a high risk of severe COVID-19 outcomes, especially those under immunosuppression or with associated comorbidities. However, few studies have assessed the safety of the COVID-19 vaccine in patients with RA. Objective To evaluate the safety of vaccines against SARS-CoV-2 in patients with RA. Methods This data are from the study “Safety and Efficacy on COVID-19 Vaccine in Rheumatic Diseases,” a Brazilian multicentric prospective phase IV study to evaluate COVID-19 vaccine in IMRDs in Brazil. Adverse events (AEs) in patients with RA of all centers were assessed after two doses of ChAdOx1 (Oxford/AstraZeneca) or CoronaVac (Sinovac/Butantan). Stratification of postvaccination AEs was performed using a diary, filled out daily and returned at the end of 28 days for each dose. Results A total of 188 patients with RA were include, 90% female. CoronaVac was used in 109 patients and ChAdOx1 in 79. Only mild AEs were observed, mainly after the first dose. The most common AEs after the first dose were pain at the injection (46,7%), headache (39,4%), arthralgia (39,4%), myalgia (30,5%) and fatigue (26,6%), and ChAdOx1 had a higher frequency of pain at the injection (66% vs 32 %, p < 0.001) arthralgia (62% vs 22%, p < 0.001) and myalgia (45% vs 20%, p < 0.001) compared to CoronaVac. The more common AEs after the second dose were pain at the injection (37%), arthralgia (31%), myalgia (23%), headache (21%) and fatigue (18%). Arthralgia (41,4% vs 25%, p = 0.02) and pain at injection (51,4% vs 27%, p = 0.001) were more common with ChAdOx1. No serious AEs were related. With Regard to RA activity level, no significant difference was observed between the three time periods for both COVID-19 vaccines. Conclusion In the comparison between the two immunizers in patients with RA, local reactions and musculoskeletal symptoms were more frequent with ChAdOx1 than with CoronaVac, especially after the first dose. In summary, the AE occurred mainly after the first dose, and were mild, like previous data from others immunizing agents in patients with rheumatoid arthritis. Vaccination did not worsen the degree of disease activity. immunemediated immune mediated (IMRDs COVID19 COVID 19 COVID-1 risks (RA outcomes comorbidities However SARSCoV2 SARSCoV SARS CoV 2 SARS-CoV- Safety Diseases, Diseases Brazil (AEs ChAdOx Oxford/AstraZeneca OxfordAstraZeneca Oxford AstraZeneca (Oxford/AstraZeneca Sinovac/Butantan. SinovacButantan Sinovac/Butantan . Sinovac Butantan (Sinovac/Butantan) diary 18 include 90 female 10 79 46,7%, 467 46,7% , 46 7 (46,7%) 39,4%, 394 39,4% 39 4 (39,4%) 30,5% 305 30 5 (30,5% 26,6%, 266 26,6% 26 6 (26,6%) 66% 66 (66 3 % 0.001 0001 0 001 62% 62 (62 22 22% 45% 45 (45 20 20% 37%, 37 37% (37%) 31%, 31 31% (31%) 23%, 23 23% (23%) 21% 21 (21% 18%. 18% (18%) 41,4% 414 41 (41,4 25 25% 0.02 002 02 51,4% 514 51 (51,4 27 27% related level summary COVID1 1 COVID- SARS-CoV (Sinovac/Butantan 9 46,7 (46,7% 39,4 (39,4% 30,5 (30,5 26,6 (26,6% (6 0.00 000 00 (4 (37% (31% (23% (21 (18% 41,4 (41, 0.0 51,4 (51, 46, (46,7 39, (39,4 30, (30, 26, (26,6 ( (37 (31 (23 (2 (18 41, (41 0. 51, (51 (46, (39, (30 (26, (3 (1 (5 (46 (39 (26

https://doi.org/10.1186/s42358-024-00397-5

Dermatological manifestations of hematologic neoplasms. Part II: nonspecific skin lesions/paraneoplastic diseases neoplasms II lesionsparaneoplastic lesions paraneoplastic
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Souza, Patricia Karla de

; Amorim, Rafael Oliveira

; Sousa, Letícia Siqueira

; Batista, Mariana Dias

Anais Brasileiros de Dermatologia

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apr 2023, Volume 98 N. 2 Pages 141 - 158

Abstract: En | Text: En | PDF: En

Abstract Cutaneous manifestations occur in the course of hematologic malignancies and precede, accompany or occur late in relation to the diagnosis. They result from paraneoplastic phenomena, tumor infiltrations, immunosuppression resulting from the hematologic disease itself or its treatment. The dermatologist must be aware of these conditions that may be helpful both in the diagnosis of the underlying disease and in reducing patient morbidity. This review (part II) addresses the paraneoplastic dermatological changes associated with systemic hematologic malignancies. precede phenomena infiltrations treatment morbidity part II

https://doi.org/10.1016/j.abd.2022.08.005

Dermatological manifestations of hematologic neoplasms. Part I: secondary specific skin lesions neoplasms I
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Souza, Patricia Karla de

; Amorim, Rafael Oliveira

; Sousa, Letícia Siqueira

; Batista, Mariana Dias

Anais Brasileiros de Dermatologia

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feb 2023, Volume 98 N. 1 Pages 5 - 12

Abstract: En | Text: En | PDF: En

Abstract Cutaneous manifestations occur during the course of hematologic malignancies and precede, follow, or are late events in relation to the diagnosis. They result from paraneoplastic phenomena, tumor infiltrations, and immunosuppression resulting from the hematologic neoplasia itself or its treatment. The dermatologist must be aware of these conditions, which can help both in the diagnosis of the underlying disease and in the reduction of patient morbidity. This review (part I) addresses skin lesions associated with direct infiltration by systemic hematologic malignancies. precede follow phenomena infiltrations treatment conditions morbidity part I

https://doi.org/10.1016/j.abd.2022.06.002

Caracterização reológica de pastas ternárias para o desenvolvimento de bioconcretos leves de casca de arroz
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Bezerra, Carolina Goulart

; Oliveira, Solanda

; Hasparyk, Nicole Paga

; Gloria, M’hamed Yassin Rajiv da

; Santos, Daniele Oliveira Justo dos

; Pereira, Alexandre de Castro

; Balthar, Vivian Karla Castelo Branco Louback Machado

; Toledo Filho, Romildo Dias

Matéria (Rio de Janeiro)

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2023, Volume 28 N. 1 elocation e20220254

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO Esta pesquisa experimental tem como objetivo produzir bioconcretos de casca de arroz (BCCs) com uma matriz de misturas ternárias de cimento, cinza da casca de arroz e cinza volante. A primeira etapa consistiu na avaliação da influência de um aditivo superplastificante e um aditivo polifuncional no comportamento reológico de pastas cimentícias. O objetivo dessa etapa foi testar diferentes teores dos aditivos a fim de desenvolver uma pasta fluida que, após a adição de altas frações volumétricas de casca de arroz (CA), permita obter BCCs leves que possuam trabalhabilidade adequada para adensamento por vibração mecânica. Realizou-se uma trituração e um tratamento químico da CA, no intuito de melhorar sua aderência com a matriz. Os resultados experimentais mostram que o aditivo do tipo PF apresentou melhor compatibilidade com a matriz a um teor de 0,3%. Assim, os BCCs produzidos apresentaram-se coesos e sem segregação, com espalhamento de 210 ± 30 mm. A massa específica seca e a resistência à compressão foram determinadas aos 28 dias de idade. Com os resultados obtidos, os BCCs apresentaram massa específica entre 1000 e 1300 kg/m3 e resistência à compressão superiores a 8,0 MPa, indicando a possibilidade de utilização na produção de painéis leves e autoportantes. (BCCs cimento volante cimentícias CA , (CA) mecânica Realizouse Realizou se 03 0 3 0,3% Assim apresentaramse segregação 21 mm 2 idade obtidos 100 130 kgm3 kgm kg m3 m kg/m 80 8 8, MPa autoportantes (CA 0,3 10 13 0, 1

ABSTRACT This experimental research aims to produce rice husk bio-concretes (RBCs) with a matrix of ternary mixtures of cement, rice husk ash and fly ash. The first step consisted in an evaluation of the influence of a superplasticizer additive and a polyfunctional additive on the rheological behavior of cementitious pastes. The purpose of this step was to test different levels of additives in order to develop a paste that, after adding high volumetric fractions of rice husk, allows obtaining RBCs that have adequate workability for compaction by mechanical vibration. A grinding and chemical treatment process was carried out on the rice husk to improve its adhesion with the matrix. The experimental results show that the PF type additive showed better compatibility with the ternary combination at an optimal content of 0.3%. Thus, the RBCs produced were cohesive, without segregation, with spreading of 210 ± 30 mm. The dry specific mass and compressive strength of the RBCs were determined after 28 days of age. Based on the results obtained, the BCCs presented specific mass between 1000 and 1300 kg/m3 and compressive strength higher than 8.0 MPa, indicating the possibility of use in the production of lightweight and load bearing panels. bioconcretes bio concretes (RBCs cement pastes vibration 03 0 3 0.3% Thus cohesive segregation 21 mm 2 age obtained 100 130 kgm3 kgm kg m3 m kg/m 80 8 8. MPa panels 0.3 10 13 0. 1

https://doi.org/10.1590/1517-7076-rmat-2022-0254

Ansiedade materna e sua interferência na autoeficácia para amamentação
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Abuchaim, Erika de Sá Vieira

; Marcacine, Karla Oliveira

; Coca, Kelly Pereira

; Silva, Isilia Aparecida

Acta Paulista de Enfermagem

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2023, Volume 36 elocation eAPE02301

Abstract: Es Pt En | Text: Es Pt En | PDF: Es | PDF: Pt | PDF: En

Resumen Objetivo Analizar la relación entre los síntomas de ansiedad materna y la autoeficacia para la lactancia y la duración de la lactancia materna exclusiva. Métodos Estudio de cohorte prospectivo, realizado en un Centro de Lactancia Materna de un hospital universitario en el municipio de São Paulo. La muestra estuvo compuesta por 83 puérperas, a quienes se les realizó un seguimiento hasta los 210 días después del parto. Se utilizó la Subescala de Ansiedad de la Escala de Depresión Posnatal de Edimburgo y la Escala de Autoeficacia para la Lactancia. Resultados El promedio de la puntuación total de ansiedad fue de 3,76 puntos y de autoeficacia materna para la lactancia 128,58. A lo largo de los meses, los síntomas de ansiedad y la autoeficacia para la lactancia presentaron un comportamiento semejante y no significativo estadísticamente. Los factores de riesgo asociados al aumento de los síntomas ansiosos son: aborto, historia previa de depresión, queja con relación a sí misma, relación familiar y conyugal insatisfactoria, deterioro en la relación con la pareja luego del nacimiento del bebé y menor autoeficacia materna para la lactancia, reflejados en los dominios técnico y pensamientos intrapersonales. El tiempo entre la interrupción de la lactancia materna exclusiva y la ansiedad no fue estadísticamente diferente. Conclusión Las puérperas investigadas presentaron una elevada prevalencia de sintomatología ansiosa, con interferencia significativa en la percepción y confianza de su capacidad y habilidad materna de amamantar con éxito al bebé. No hubo asociación entre los síntomas de ansiedad y el destete temprano. prospectivo Paulo 8 21 parto 376 3 76 3,7 12858 128 58 128,58 meses son aborto depresión misma insatisfactoria intrapersonales diferente ansiosa temprano 2 37 7 3, 1285 12 5 128,5 1 128,

Resumo Objetivo Analisar a relação entre os sintomas de ansiedade materna com a autoeficácia para a amamentação e a duração do aleitamento materno exclusivo. Métodos Estudo de coorte prospectivo, desenvolvido num Centro de Aleitamento Materno de um hospital universitário do município de São Paulo. A amostra foi constituída por 83 puérperas, acompanhadas até 210 dias após o parto. Foram utilizadas a Subescala de Ansiedade da Escala de Depressão Pós-natal de Edimburgo e a Escala de Autoeficácia para Amamentação. Resultados A média de escore total de ansiedade foi 3,76 pontos e da autoeficácia materna para amamentar 128,58. Ao longo dos meses os sintomas de ansiedade e a autoeficácia para amamentação apresentaram comportamento semelhante e não significativos estatisticamente. São fatores de risco associados ao incremento dos sintomas ansiosos: aborto, história prévia de depressão, queixa em relação a si mesma, relacionamento familiar e conjugal insatisfatório, piora na relação com o parceiro após o nascimento do bebê e menor autoeficácia materna para amamentação, refletidos nos domínios técnico e pensamentos intrapessoais. O tempo entre a interrupção do aleitamento materno exclusivo e a ansiedade não foi estatisticamente diferente. Conclusão As puérperas investigadas apresentaram elevada prevalência de sintomatologia ansiosa, com interferência significativa na percepção e confiança em sua capacidade e habilidade materna de amamentar com sucesso o bebê. Não houve associação entre os sintomas de ansiedade e o desmame precoce. prospectivo Paulo 8 21 parto Pósnatal Pós natal Amamentação 376 3 76 3,7 12858 128 58 128,58 ansiosos aborto depressão mesma insatisfatório intrapessoais diferente ansiosa precoce 2 37 7 3, 1285 12 5 128,5 1 128,

Abstract Objective To analyze the relationship of maternal anxiety symptoms with breastfeeding self-efficacy and duration of exclusive breastfeeding. Methods Prospective cohort study developed in a Breastfeeding Center of a university hospital in the city of São Paulo. The sample consisted of 83 postpartum women in follow-up for 210 days after delivery. The Anxiety Subscale of the Edinburgh Postnatal Depression Scale and the Breastfeeding Self-Efficacy Scale were used. Results The mean total score for anxiety was 3.76 points and for maternal self-efficacy to breastfeed was 128.58. Over the months, anxiety symptoms and breastfeeding self-efficacy showed similar behavior and were not statistically significant. The following risk factors were associated with the increase in anxiety symptoms: abortion, previous history of depression, complaints about oneself, unsatisfactory family and marital relationships, worsening of the relationship with the partner after the baby was born and lower maternal breastfeeding self-efficacy, reflected in the technique domain and intrapersonal thoughts domain. The time between cessation of exclusive breastfeeding and anxiety was not statistically different. Conclusion The investigated postpartum women had a high prevalence of anxiety symptoms, with significant interference in the perception and confidence in their ability and maternal capability to successfully breastfeed the baby. There was no association between anxiety symptoms and early breastfeeding cessation. selfefficacy self efficacy Paulo 8 followup follow up 21 delivery SelfEfficacy Self Efficacy used 376 3 76 3.7 12858 128 58 128.58 months abortion depression oneself relationships selfefficacy, efficacy, different 2 37 7 3. 1285 12 5 128.5 1 128.

https://doi.org/10.37689/acta-ape/2023ao02301

Factors associated with absenteeism in elderly nursing workers
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Ferreira, Arianne Gueiros

; Lopes, Maysa Rayanne Cardozo

; Borba, Anna Karla de Oliveira Tito

; Marques, Ana Paula de Oliveira

; Alves, Fábia Alexandra Pottes

; Lima, Bruna Cyreno de Carvalho

Revista Gaúcha de Enfermagem

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2022, Volume 43 elocation e20210063

Abstract: Pt En Es | Text: Pt En Es | PDF: Pt | PDF: En | PDF: Es

RESUMO Objetivo: Analisar os fatores associados ao absenteísmo em trabalhadores idosos de enfermagem. Métodos: Estudo transversal com 233 trabalhadores idosos de enfermagem realizado em seis hospitais do Recife, Pernambuco - BR. A análise de dados foi univariada e regressão multivariada. Resultados: Dos 233 profissionais de enfermagem idosos entrevistados, o absenteísmo autorreferido esteve presente em 34,3% (80) dos participantes. From them, 20.1% (47) reported having missed few days, while 14.2% (33) stated to have missed many days. Na análise ajustada, apenas as horas trabalhadas e a satisfação com a saúde influenciaram o absenteísmo. Conclusão: O absenteísmo é um fenômeno complexo e entre os profissionais de enfermagem idosos apresentou baixa prevalência e esteve associado a carga horária de trabalho acima de 40 horas semanais e a insatisfação com a saúde. Portanto, se faz necessário novas pesquisas na área para fornecer qualidade de vida e melhores condições de trabalho aos profissionais de enfermagem e regulamentação de sua carga horária.

ABSTRACT Objective: To analyze the factors associated with absenteeism in elderly nursing workers. Methods: Cross-sectional study with 233 elderly nursing workers carried out in six hospitals in Recife, Pernambuco, Brazil. The data were analyzed using univariate analysis and multivariate regression. Results: Of the 233 elderly nursing professionals interviewed, self-reported absenteeism was present in 34.3% (80) participants. 20.1% (47) of them reported having missed a few days and 14.2% (33) having done so in many days. After adjusted analysis, only work hours and satisfaction with health influenced absenteeism. Conclusion: Absenteeism is a complex phenomenon which, among elderly nursing professionals, had a low prevalence and was associated with a workload of more than 40 hours a week and dissatisfaction with health. Therefore, further research is needed in the field to provide quality of life and better working conditions for nursing professionals and regulate their workload.

RESUMEN Objetivo: Analizar los factores asociados al absentismo en trabajadores de enfermería ancianos. Métodos: Estudio transversal con 233 trabajadores de enfermería de edad avanzada realizado en seis hospitales de Recife, Pernambuco, Brasil. Los datos se analizaron mediante análisis univariado y regresión multivariada. Resultados: De los 233 profesionales de enfermería ancianos entrevistados, el ausentismo autonotificado estuvo presente en el 34,3% (80) de los participantes. De estos, el 20,1% (47) informó haber perdido algunos días y el 14,2% (33) muchos días. En el análisis ajustado, solo las horas trabajadas y la satisfacción con la salud influyeron en el absentismo. Conclusión: El absentismo es un fenómeno complejo y entre los profesionales de enfermería ancianos tuvo baja prevalencia y se asoció con una carga de trabajo de más de 40 horas a la semana y la insatisfacción con la salud. Por lo tanto, más investigaciones sobre el tema son necesarias para traer calidad de vida y mejores condiciones laborales a los profesionales de enfermería y regular su carga de trabajo.

https://doi.org/10.1590/1983-1447.2022.20210063.en

Amamentação em menores de dois anos em uma cidade da Região Amazônica
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Maciel, Vanizia Barboza da Silva

; Abuchaim, Érika de Sá Vieira

; Maia, Raquel da Rocha Paiva

; Coca, Kelly Pereira

; Marcacine, Karla Oliveira

; Abrão, Ana Cristina Freitas de Vilhena

Acta Paulista de Enfermagem

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2022, Volume 35 elocation eAPE02487

Abstract: Pt En Es | Text: Pt En Es | PDF: Pt | PDF: En | PDF: Es

Resumo Objetivo Analisar a duração do aleitamento materno e os fatores associados ao desmame total de crianças de seis a 23 meses e 29 dias de idade residentes no município de Cruzeiro do Sul, na Amazônia Ocidental Brasileira. Métodos Estudo transversal, realizado durante a Campanha Nacional de Multivacinação em 2016 e Contra a Influenza em 2017. A amostra foi calculada por conglomerados. A coleta de dados foi efetuada com as mães ou os responsáveis de 679 crianças que compareceram às campanhas de vacinação e responderam a um questionário. Utilizou-se a análise de sobrevivência de Kaplan-Meier e, para os fatores associados ao desmame total, a regressão de Cox. Para todos os testes estatísticos, foi considerado um nível de significância de 5%. Resultados O aleitamento materno foi praticado por 65,3% das crianças, cuja média de idade foi de 13,7 meses (DP± 4,9 meses). O tempo médio de desmame total foi de 16,7 meses (IC95%: 16,06 - 17,36) e a mediana de 22 meses, sendo a probabilidade de tempo de aleitamento materno até dois anos em 49,7%. Os fatores associados ao desmame total foram o tempo da experiência anterior em amamentação menor que seis meses, não praticar o aleitamento materno na primeira hora de vida, uso de chupeta e mamadeira. Conclusão A duração do aleitamento materno foi abaixo do recomendado. Os fatores associados ao desmame total de crianças entre 6 e 23 meses estão relacionados à experiência materna prévia, ao início precoce da prática de amamentação e ao uso de bicos artificiais.

Abstract Objective To analyze the duration of breastfeeding and the factors associated with total weaning of infants aged six to 23 months and 29 days living in the municipality of Cruzeiro do Sul, in the Brazilian Western Amazon. Methods A cross-sectional study conducted during the National Multi-Vaccination Campaign in 2016 and Against Influenza in 2017. The sample was estimated by clusters. The data was collected with the mothers or guardians of 679 infants who attended vaccination campaigns and answered a questionnaire. Kaplan-Meier survival analysis was used, and Cox regression was used for the factors associated with total weaning. A 5% significance level was considered for all statistical tests. Results Breastfeeding was practiced by 65.3% of the infants, whose mean age was 13.7 months (SD± 4.9 months). The mean total weaning time was 16.7 months (95% CI: 16.06 - 17.36) and the median of 22 months, which is the probability of duration of breastfeeding up to two years old in 49.7%. The factors associated with total weaning were previous breastfeeding experience for less than six months, not breastfeeding in the first hour of life, pacifier use and bottle-feeding. Conclusion The duration of breastfeeding was below the recommended. The factors associated with total weaning of infants between six and 23 months are related to previous maternal experience, early initiation of breastfeeding and the use of artificial nipples.

Resumen Objetivo Analizar la duración de la lactancia materna y los factores asociados al destete total de niños de seis meses a 23 meses y 29 días de edad que viven en el municipio de Cruzeiro do Sul, en la Amazonía Occidental Brasileña. Métodos Estudio transversal, realizado durante la Campaña Nacional de Multivacunación en 2016 y Contra la Influenza en el 2017. La muestra fue calculada por conglomerados. La recopilación de datos se realizó con las madres o los responsables de 679 niños que asistieron a las campañas de vacunación y respondieron un cuestionario. Se utilizó el análisis de supervivencia de Kaplan-Meier y, para los factores asociados al destete total, la regresión de Cox. Para todas las pruebas estadísticas se consideró un nivel de significación del 5 %. Resultados La lactancia materna se practicó en el 65,3 % de los niños, cuyo promedio de edad fue de 13,7 meses (DP± 4,9 meses). El tiempo promedio de destete total fue de 16,7 meses (IC95 %: 16,06 - 17,36) y la mediana de 22 meses, con una probabilidad de tiempo de lactancia materna hasta los dos años del 49,7 %. Los factores asociados al destete total fueron el tiempo de una experiencia anterior de lactancia inferior a seis meses, no practicar la lactancia materna en la primera hora de vida, uso de chupete y de mamadera. Conclusión La duración de la lactancia materna estuvo por debajo de lo recomendado. Los factores asociados al destete total de niños entre 6 y 23 meses están relacionados a experiencias maternas previas, al inicio precoz de la práctica de la lactancia y al uso de tetinas artificiales.

https://doi.org/10.37689/acta-ape/2022ao02487

Laboratory-based study of drug resistance and genotypic profile of multidrug-resistant tuberculosis isolates in Salvador, Bahia, Brazil
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Sousa, Erivelton de Oliveira

; Carneiro, Rita Terezinha de Oliveira

; Montes, Fátima Cristina Onofre Fandinho

; Conceição, Emilyn Costa

; Bartholomay, Patricia

; Marinho, Jamocyr Moura

; Lima, Karla Valéria Batista

; Natividade, Marcio Santos da

; Araújo, Wildo Navegantes de

; Matos, Eliana Dias

; Barbosa, Theolis

Revista da Sociedade Brasileira de Medicina Tropical

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2022, Volume 55 elocation e0013-2022

Abstract: En | Text: En | PDF: En

Abstract Background: Surveillance of multidrug resistant/extensively drug-resistant tuberculosis (MDR/XDR-TB) is essential to guide disease dissemination control measures. Brazil contributes to a significant fraction of tuberculosis (TB) cases worldwide, but only few reports addressed MDR/XDR-TB in the country. Methods: This cross-sectional, laboratory-based study describes the phenotypic resistance profiles of isolates obtained between January 2008 and December 2011 in Bahia, Brazil, and sociodemographic, epidemiological, and clinical characteristics (obtained from mandatory national registries) of the corresponding 204 MDR/XDR-TB patients. We analyzed the mycobacterial spoligotyping and variable number of tandem repeats of mycobacterial interspersed repetitive units in 12-loci profiles obtained from Salvador. Results: MDR/XDR-TB patients were predominantly male, had a median age of 43 years, belonged to black ethnicity, and failed treatment before MDR-TB diagnosis. Nearly one-third of the isolates had phenotypic resistance (evaluated by mycobacteria growth indicator tube assay) to second-line anti-TB drugs (64/204, 31%), of which 22% cases (14/64) were diagnosed as XDR-TB. Death was a frequent outcome among these individuals and was associated with resistance to second-line anti-TB drugs. Most isolates successfully genotyped belonged to the Latin-American Mediterranean (LAM) Family, with an unprecedented high proportion of LAM10-Cameroon subfamily bacilli. More than half of these isolates were assigned to a unique cluster by the genotyping methods performed. Large clusters of identical genotypes were also observed among LAM SIT42 and SIT376 strains. Conclusions: We highlight the need for strengthening local and national efforts to perform early detection of TB drug resistance and to prevent treatment discontinuation to limit the emergence of drug-resistant strains.

https://doi.org/10.1590/0037-8682-0013-2022

Visceral leishmaniasis: historical series of hospitalized patients and correlation with climate in an endemic area in Minas Gerais, Brazil
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Martins, Igor M. L.

; Silva, Jescilene S.

; Campos, Danielle Karla O.

; Oliveira, Ricardo S.

; Silva, Patrick Leonardo N.

; Carvalho, Silvio Fernando G.

; Caldeira, Antônio P.

Jornal Brasileiro de Patologia e Medicina Laboratorial

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2021, Volume 57 elocation e2702021

Abstract: Pt En Es | Text: Pt En Es | PDF: Pt | PDF: En | PDF: Es

RESUMEN Introducción La leishmaniasis visceral (LV) es una enfermedad infecciosa causada por protozoos del género Leishmania; predomina en las regiones tropicales, subtropicales y templadas. Objetivo Caracterizar una serie de casos de LV en pacientes mayores de 18 años atendidos en un hospital de referencia del norte de Minas Gerais, así como describir la ocurrencia de casos, según la caracterización climatológica, y correlacionar la distribución de los casos con la lluvia y la temperatura ambiente. Métodos El diseño del estudio fue una serie de casos recopilados entre 1999 y 2016. Los datos se extrajeron de historias clínicas seleccionadas cronológicamente. La información climática se recopiló en el Centro de estudios del Semiárido de la Universidad Federal de Montes Claros (Unimontes). Resultados Hubo un ligero predominio del sexo masculino (54,4%) y de edades comprendidas entre uno y cuatro años (42,9%). El tiempo de evolución de las manifestaciones clínicas hasta el momento de la hospitalización osciló entre cinco y 120 días, siendo la duración más frecuente entre 16 y 30 días (34,4%). Hepatomegalia, esplenomegalia, palidez cutánea y fiebre fueron manifestaciones presentes en más del 90% de los pacientes al ingreso. El diagnóstico se realizó por métodos serológicos en la mayoría de los casos (85,9%). La mayor parte de los ingresos duró entre 16 y 30 días (44%) y el alta hospitalaria con orientación para el control ambulatorio fue el resultado más frecuente de los ingresos. El porcentaje de muertes fue del 2%. Conclusión Las características predominantes son niños varones de 1 a 4 años, que acudió al servicio de salud con la clásica tríada LV: hepatoesplenomegalia, palidez y fiebre prolongada. En este estudio no hubo asociación estadísticamente significativa entre temperatura, precipitación y número de casos, sin embargo, existen matices en los factores ambientales que influyen en la dinámica de transmisión de LV, los cuales varían de región a otra.

https://doi.org/10.5935/1676-2444.20210045 1 downloads

10.

Orofacial antinociceptive activity and anchorage molecular mechanism in silico of geraniol
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COSTA, Tereza Karla Vieira Lopes da

; BARROS, Mariana Silva

; BRAGA, Renan Marrinho

; VIANA, Jéssika de Oliveira

; SOUSA, Frederico Barbosa de

; SCOTTI, Luciana

; SCOTTI, Marcus Tullius

; BATISTA, André Ulisses Dantas

; ALMEIDA, Reinaldo Nóbrega de

; CASTRO, Ricardo Dias de

Brazilian Oral Research

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2020, Volume 34 elocation e094

Abstract: En | Text: En | PDF: En

Abstract We aimed to evaluate the orofacial antinociceptive effect of geraniol in mice and its molecular anchorage mechanism. Seven mice per group (probabilistic sample) were treated with geraniol (12.5, 25 and 50 mg/kg, i.p.), morphine (6 mg/kg, i.p.) and vehicle (saline + Tween 80 at 0.2%, i.p.) 30 minutes prior to the beginning of the experiment. Injecting glutamate (25 μM), capsaicin (2.5 μg) and formalin (2%) into the right upper lip (perinasal) of the mouse induced nociception. Behavioral analysis of the animals considered the friction time (in seconds) of the mentioned region using hind or front paws by a researcher blinded to the treatment groups. The statistical analysis was performed blindly, considering α = 5%. The results showed that in the glutamate and capsaicin tests, concentrations of 25 mg/kg and 50 mg/kg presented antinociceptive activity (p < 0.005, power> 80%). In the formalin test, geraniol was able to reduce nociception at a concentration of 50 mg/kg (p < 0.005, power> 80%). In the molecular anchorage study, high values of binding between the evaluated substance and receptors of glutamate were observed (metabotropic glutamate receptor, -87.8501 Kcal/mol; N-methyl-D-aspartate, -86.4451 Kcal/mol; α-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid, -85.6755 Kcal/mol). Geraniol presented orofacial antinociceptive activity, probably by interacting with glutamate-related receptors.

https://doi.org/10.1590/1807-3107bor-2020.vol34.0094 467 downloads

11.

Analysis of Estrogen Effects on Eruption of Rat Molars
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Lima-Pansini, Livia Fiorim de

; Lima, Nayra de Souza Carvalho

; Guss, Natan Oliveira

; Alves, Izabela Sinara Silva

; Gama-de-Souza, Letícia Nogueira da

; Gracelli, Jones Bernardes

; Pacheco, Marcos da Silva

; Coburn, Karla Loureiro Almeida

International journal of odontostomatology

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dec 2019, Volume 13 N. 4 Pages 418 - 427

Abstract: En Es | Text: En Es | PDF: En | PDF: Es

ABSTRACT: Tooth eruption requires resorption of the alveolar bone interposed between the tooth germ and the oral mucosa (coronal bone). The cells responsible for bone resorption are the osteoclasts and their activity can be reduced or inactivated by estrogen hormone. We aimed to investigate the effects of estrogen on the process of tooth eruption in rats. Thirty-three Wistar rats, aged two-to-17-days, were divided into control, sham and estrogen-treated groups. After daily injections with estrogen, the animals were euthanized and the jaws removed and processed for histological analysis. We performed clinical examination, morphological analysis, quantification of the number of osteoclasts on the surface of the coronal bone and immunohistochemical analysis of estrogen receptor type alpha (ERα). Estrogen therapy was effective, which could be confirmed by the higher estrogen plasma levels on treated animals. However, it had no effect on tooth development or tooth eruption. Progressive bone resorption was observed and the number of osteoclasts on coronal bone was not affected on hormoneinjected animals, allowing tooth to erupt at the same time observed in untreated animals. Immunohistochemistry for ERα confirmed the presence of this type of receptor in osteoclasts, osteoblasts and osteocytes. Taken together, our results showed that estrogen stimulation was not sufficient to decrease the number of osteoclasts on the coronal bone, supporting the idea that, although estrogen may have a protective activity on bone resorption, this may not apply to the alveolar bone that is meant to be resorbed during eruptive process.

RESUMEN: La erupción dental requiere la resorción del hueso alveolar interpuesto entre el germen dental y la mucosa oral (hueso coronal). Las células responsables de la resorción ósea son los osteoclastos y su actividad puede reducirse o inactivarse por la hormona del estrógeno. Objetivos: apuntamos a investigar los efectos del estrógeno en el proceso de la erupción dental en ratas. Treinta y tres ratas Wistar, de dos a 17 días de edad, se dividieron en grupos de control, Sham y se trataron con estrógenos. Los animales fueron eutanizados después del tratamento con estrógeno y se procesaron las mandíbulas para el análisis histológico. Se realizó el examen clínico, el análisis morfológico, la cuantificación del número de osteoclastos en la superficie del hueso coronal y el análisis inmunohistoquímico del tipo de receptor de estrógeno alfa (ERα). La terapia de estrógeno fue eficaz, lo que podría ser confirmado por los niveles plasmáticos más altos de estrógeno en los animales tratados. Sin embargo, no se observó ningún efecto sobre el desarrollo de los dientes o la erupción dental. Se observó una resorción ósea progresiva y el número de osteoclastos en el hueso coronal no se vio afectado en los animales inyectados con hormonas, permitiendo que el diente erupcionó durante el mismo período de tiempo observado en animales no tratados. La inmunohistoquímica para el ERα confirmó la presencia de este tipo de receptor en los osteoclastos, osteoblastos y osteocitos. Nuestros resultados mostraron que la estimulación del estrógeno no fue suficiente para reducir el número de osteoclastos en el hueso coronal confirmando que, si bien el estrógeno puede tener una actividad protectora en la resorción ósea, esto puede no se aplica al hueso alveolar que está destinado a ser rerecurrido durante el proceso eruptivo.

521 downloads

12.

Evidence of bovine viral diarrhea virus transmission by back pond water in experimentally infected piglets
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Nascimento, Karla A.

; Mechler, Marina L.

; Gatto, Igor R.H.

; Almeida, Henrique M.S.

; Pollo, Andressa S.

; Sant’Ana, Fabiano J.F.

; Pedroso, Pedro M.O.

; Oliveira, Luís G. de

Pesquisa Veterinária Brasileira

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oct 2018, Volume 38 N. 10 Pages 1896 - 1901

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO: Os suínos podem ser infectados pelo vírus da diarreia viral bovina (BVDV). No entanto, as vias de transmissão entre os suínos são ainda desconhecidas. O objetivo do presente estudo foi induzir a infecção experimental de BVDV-1 em leitões desmamados e avaliar a potencial transmissão pela lâmina d’água, que ajuda na troca de calor dos suínos alojados em baias. Duas repetições do experimento (BP1 e BP2) foram realizadas com 12 animais comprovadamente livres de BVDV (n=6 por repetição) alocados em três grupos: controle, sentinelas e infectados, com dois animais cada. Os animais foram mantidos em isoladores de aço inoxidável. O grupo infectado recebeu um inóculo contendo BVDV-1, estirpe Singer. Os animais permaneceram nos isoladores durante 25 dias e, durante esse período, amostras de suabe nasal foram coletadas diariamente e sangue coletado semanalmente. No final, os animais foram eutanasiados, necropsiados e fragmentos de órgãos foram coletados para histopatologia, imuno-histoquímica e RT-PCR. No primeiro experimento (BP1), os animais infectados excretaram partículas virais entre os dias 6 e 21 pós-infecção. Quanto ao grupo sentinela, a excreção ocorreu apenas em um animal, no 20º dia pós-infecção, e a soroconversão foi observada no 25º dia pós-infecção. Na BP2, os animais infectados I3 e I4 excretaram partículas virais nos dias 4 e 21 pós-infecção, respectivamente. Apenas um animal sentinela (S3) apresentou excreção no dia 13 pós-infecção. Concluiu-se que os suínos podem se infectar com BVDV-1 e excretar partículas virais potencialmente infecciosas, sendo capazes de transmitir o vírus a outros suínos através da lâmina d’água.

ABSTRACT: Swine can be infected by bovine viral diarrhea virus (BVDV). However, transmission routes among pigs are still unknown. The objective of the present study was to induce experimental infection of BVDV-1 in weaned piglets and to assess the potential transmission through pen back pond water, used to facilitate heat exchange of the pigs housed in barns. Two repetitions (BP1 and BP 2) were performed using 12 piglets proven to be free BVDV (n=6 per repetition) allocated into three groups: control, sentinels and infected with two piglets each. The piglets were placed in stainless steel isolators. The infected group received an inoculum containing BVDV-1, Singer strain. The piglets remained in the cabinets for 25 days, during which samples of nasal swab were collected daily and blood sampled weekly. At the end, the piglets were euthanized, necropsied and organ fragments were collected for histopathology, immunohistochemistry and RT-PCR. In the first experiment (BP1) the infected animals shed the virus between days 6 and 21 post-infection. Regarding the sentinel group, shedding occurred in only one piglet, on the 20th day after infection, and seroconversion was observed on the 25th day post-infection. In BP2, infected piglets I3 and I4 shed the virus on days 4 and 21 post-infection, respectively. Only one sentinel piglet (S3) she the virus on day 13 post-infection. Therefore, it was concluded that pigs can become infected with BVDV-1 and shed potentially infectious viral particles consequently, being able to transmit the virus to other pigs through back pond water.

https://doi.org/10.1590/1678-5150-pvb-5629 622 downloads

13.

Dificuldades relacionadas ao aleitamento materno: análise de um serviço especializado em amamentação
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Carreiro, Juliana de Almeida

; Francisco, Adriana Amorim

; Abrão, Ana Cristina Freitas de Vilhena

; Marcacine, Karla Oliveira

; Abuchaim, Erika de Sá Vieira

; Coca, Kelly Pereira

Acta Paulista de Enfermagem

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aug 2018, Volume 31 N. 4 Pages 430 - 438

Abstract: En Es Pt | Text: En Es Pt | PDF: En | PDF: Es | PDF: Pt

Abstract Objective To analyze the association between the type of breastfeeding and the difficulties related to this practice among women and children assisted in a clinic specialized in breastfeeding. Methods Cross-sectional retrospective study based on the analysis of medical records of women and children assisted in a clinic specialized in breastfeeding from 2004 to 2016. Medical records of women with multiple pregnancies and documents registered as nonstandard forms were excluded, which resulted in a sample with 1,608 records. The chi-square, nonparametric Mann-Whitney, and nonparametric Kruskal-Wallis tests were applied to compare the type of breastfeeding with categorical variables, frequency of exclusive breastfeeding, and days of life and maternal age, respectively. Results Exclusive breastfeeding was practiced by 72.6% of the assisted women in the first 30 days after childbirth. There was a significant association between this practice and the following difficulties: maternal perception regarding the volume of produced milk, full breasts before breastfeeding, milk leakage, and easy manual milk extraction; proper positioning of mother and child, latch, suction, and swallowing by the child; in addition to the following characteristics: higher level of education; stable marital status; previous experience with breastfeeding; protruding nipples; early skin-to-skin contact with the child; and children with a lower average number of days of life and who used pacifiers. Conclusion Exclusive breastfeeding prevailed in the first 30 days after childbirth and several maternal and neonatal variables were associated with this practice in the first appointment in the specialized clinic.

Resumen Objetivo Analizar la asociación entre el tipo de amamantamiento y las dificultades relacionadas con esta práctica entre las mujeres y los niños atendidos en una clínica especializada en la lactancia materna. Métodos Estudio transversal retrospectivo llevado a cabo por medio del análisis de expedientes de niños y mujeres atendidas entre 2004 y 2016, en una clínica especializada en lactancia materna. Se excluyeron los registros referentes a las mujeres con gestación múltiple y a los no realizados de forma estándar, totalizando 1.608 expedientes. Se utilizaron las pruebas Qui-cuadrado y Kruskal-Wallis para comparar el tipo de lactancia materna con variables categóricas; y con los días de vida y edad materna, respectivamente. La prueba Mann-Whitney fue utilizada para comparar la frecuencia de la lactancia materna exclusiva. Resultados La lactancia exclusiva fue practicada por el 72,6% de las mujeres atendidas en los primeros 30 días después del parto. Se observó una asociación significativa entre esta práctica y las dificultades: percepción materna en cuanto a la cantidad de leche producida, de mamas llenas antes de las tomas, de fuga de leche y extracción manual de leche con facilidad; posicionamiento materno y del niño, prensión, succión y deglución del niño adecuados; además de las variables: mayor escolaridad, situación conyugal estable; haber tenido experiencia previa con la lactancia materna, tener pezones sobresalientes, haber realizado contacto precoz piel a piel, tener hijos con menor promedio de días de edad y que hacían uso de chupete. Conclusión La lactancia materna exclusiva fue lo más prevalente en los primeros 30 días después del parto y diversas variables maternas y neonatales fueron asociados con esta práctica en la primera atención en clínicas especializadas.

Resumo Objetivo Analisar a associação entre o tipo de aleitamento e as dificuldades relacionadas à essa prática entre mulheres e crianças assistidas em um ambulatório especializado em amamentação. Métodos Estudo transversal retrospectivo realizado por meio da análise de prontuários de crianças e mulheres atendidas entre 2004 e 2016 em um ambulatório especializado em aleitamento materno. Foram excluídos os registros referentes às mulheres com gestação múltipla e àqueles não realizados em formulário padrão, totalizando 1.608 prontuários. Utilizaram-se os testes Qui-Quadrado e Kruskal-Wallis para comparar o tipo de aleitamento materno com variáveis categóricas; e com os dias de vida e idade materna, respectivamente. O teste Mann-Whitney utilizou-se para comparar a frequência do aleitamento materno exclusivo. Resultados O aleitamento materno exclusivo foi praticado por 72,6% das mulheres atendidas, nos primeiros 30 dias após o parto. Houve associação significativa entre esta prática e as dificuldades: percepção materna quanto à quantidade de leite produzida, de mamas cheias antes das mamadas, de vazamento de leite e extração manual do leite com facilidade; posicionamento materno e da criança, preensão, sucção e deglutição da criança adequados; além das variáveis: maior escolaridade, situação conjugal estável; ter tido experiência prévia com aleitamento materno, ter mamilos protrusos, ter realizado contato precoce pele a pele, ter filhos com menor média de dias de idade e que faziam uso de chupeta. Conclusão O aleitamento materno exclusivo foi o mais prevalente nos primeiros 30 dias pós-parto e diversas variáveis maternas e neonatais estiveram associadas à essa prática no primeiro atendimento em ambulatório especializado.

https://doi.org/10.1590/1982-0194201800060 2 downloads

14.

Dificuldades relacionadas ao aleitamento materno: análise de um serviço especializado em amamentação
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Carreiro, Juliana de Almeida

; Francisco, Adriana Amorim

; Abrão, Ana Cristina Freitas de Vilhena

; Marcacine, Karla Oliveira

; Abuchaim, Erika de Sá Vieira

; Coca, Kelly Pereira

Acta Paulista de Enfermagem

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jul 2018, Volume 31 N. 4 Pages 430 - 438

Abstract: Pt En Es | Text: Pt En Es | PDF: Pt | PDF: En | PDF: Es

https://doi.org/10.1590/1982-0194201800060 14826 downloads

15.

Densidade mineral óssea em vértebras de ratos submetidos a imobilização e terapia aquática
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Rodrigues, Daiana Cordeiro

; Souza, Karla Camila Lima de

; Souza, Mateus Bastos de

; Ceccatto, Vânia Marilande

; Santos Júnior, Francisco Fleury Uchoa

; Oliveira, Cristiane Mattos de

Motricidade

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may 2018, Volume 14 N. 1 Pages 346 - 350

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

A imobilização pode acarretar uma diminuição na densidade mineral óssea (DMO) do membro acometido e a terapia aquática pode ser considerada uma forma de tratamento. O objetivo do presente estudo foi analisar a DMO em vértebras de ratos submetidos à imobilização e posteriormente tratados com terapia aquática. Foram utilizados 32 ratos, fêmeas, Wistar, divididas em quatro grupos: controlo (C), imobilizado (I), imobilizado/terapia aquática (ITA) e terapia aquática (TA). A imobilização ocorreu no membro posterior direito, incluindo a pelve, o quadril, fémur, o joelho (extensão), tíbia e tornozelo (flexão plantar) durante duas semanas. A terapia aquática ocorreu durante seis dias por semana ao longo de duas semanas. Após esse período os animais foram sacrificados e dissecados as vértebras L6 e T13 e posteriormente analisado a DMO. Verificou-se que houve uma diminuição na DMO na vértebra T13 do grupo I em relação ao C, e um aumento significativo no grupo ITA em relação ao I. Esses resultados demonstraram que a terapia aquática é capaz de restaurar a perda óssea oriunda da imobilização.

Immobilization decrease in the bone mineral density (BMD) of body segment that is immobilized, and aquatic therapy has often been considered as a possible treatment. The aim off the present study was to compare spine BMD of rats that underwent immobilization and subsequently followed aquatic therapy. 32 female Wistar rats were divided into 4 groups: control, immobilized, immobilized + aquatic therapy and aquatic therapy. Immobilization was performed in the rear right limb, comprising pelvis, hip, leg, knee and ankle and lasted 2 weeks. Aquatic therapy lasted 3 weeks, 6 days per week. After the experiment the rats were sacrificed and vertebrae L6 and T13 were removed and analyzed for BMD. There was a decrease in BMD of T13 in the immobilized group when compared with control and an increase in immobilized + aquatic therapy group when compared with immobilized group. It is concluded that aquatic therapy has the potential to override bone loss induced by limb immobilization.

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