Resumo Introdução: A doença renal crônica costuma ser assintomática e seu diagnóstico depende da realização de exames laboratoriais, com destaque para a creatinina sérica e pesquisa de proteinúria. Objetivo: Avaliar em uma amostra da população brasileira o conhecimento sobre o papel da creatinina sérica como marcador de função renal. Método: Estudo observacional transversal realizado na cidade de São Paulo (SP, Brasil), em que foi entrevistada uma população adulta aleatória. Resultados: Foram entrevistados 1138 indivíduos, com idade mediana de 36 anos (27–52); 55,1% do sexo feminino. Com relação ao marcador “creatinina”, 40,6% afirmaram que nunca realizaram tal dosagem. Quando questionados quanto ao conhecimento sobre a utilidade desse exame, somente 19,6% sabiam a sua função. As outras respostas foram “não sei” (71,6%), avaliar o funcionamento do coração (0,9%) e fígado (7,8%). Dos que afirmaram já terem realizado o exame de creatinina, somente 29,4% acertaram a função da creatinina. Ao dividir os grupos em “sabe” e “não sabe” a função da creatinina, percebeu-se diferença estatisticamente significante (p < 0,05) em relação ao grau de escolaridade, sexo feminino, ser aluno/trabalhador da saúde, ter dosado creatinina alguma vez, conhecer alguém com doença renal e maior idade. Na análise multivariada, a principal variável relacionada com conhecer a função da creatinina foi ter realizado o exame anteriormente (OR 5,16; IC 95% 3,16–8,43, p < 0,001). Conclusão: Há grande desconhecimento sobre a creatinina e seu uso em check-ups. Os resultados indicam que é necessário maior esforço por parte dos profissionais de saúde para divulgar o papel da creatinina sérica. Introdução laboratoriais proteinúria Objetivo Método SP, SP (SP Brasil, Brasil , Brasil) aleatória Resultados 113 indivíduos 3 27–52 2752 27 52 (27–52) 551 55 1 55,1 feminino “creatinina” 406 40 6 40,6 dosagem 196 19 19,6 não sei 71,6%, 716 71,6% 71 (71,6%) 0,9% 09 0 9 (0,9% 7,8%. 78 7,8% . 7 8 (7,8%) 294 29 4 29,4 sabe “sabe percebeuse percebeu se 0,05 005 05 escolaridade alunotrabalhador aluno trabalhador vez multivariada OR 5,16 516 5 16 95 316843 43 3,16–8,43 0,001. 0001 0,001 001 0,001) Conclusão checkups. checkups check ups. ups check-ups 11 27–5 275 2 (27–52 55, “creatinina 40, 19, 71,6 (71,6% 0,9 (0,9 7,8 (7,8% 29, 0,0 00 5,1 51 31684 3,16–8,4 000 0,00 27– (27–5 71, (71,6 0, (0, 7, (7,8 5, 3168 3,16–8, (27– (71, (0 (7, 316 3,16–8 (27 (71 ( (7 31 3,16– (2 3,16 3,1 3,

Abstract Introduction: Chronic kidney disease is usually asymptomatic, and its diagnosis depends on laboratory tests, with emphasis on serum creatinine and proteinuria. Objective: To assess knowledge on the role of serum creatinine as a biomarker of kidney function in a sample of the Brazilian population. Method: Cross-sectional observational study conducted in São Paulo (SP, Brazil), in which a random adult population was interviewed. Results: A total of 1138 subjects were interviewed, with a median age of 36 years old (27–52); 55.1% were female. Regarding the “creatinine” biomarker, 40.6% stated they had never performed such a test. When asked about their knowledge on the usefulness of this exam, only 19.6% knew its function. The other responses were “I don’t know” (71.6%), evaluating heart function (0.9%) and liver function (7.8%). Of those who reported they had already taken a creatinine test, only 29.4% correctly identified the role of creatinine. When dividing the groups into “knows” and “does not know” the function of creatinine, a statistically significant difference (p < 0.05) was observed regarding level of education, female sex, being a healthcare student/worker, having ever measured creatinine, knowing someone with kidney disease and older age. In the multivariate analysis, the main variable related to knowing the creatinine role was having previously taken the test (OR 5.16; 95% CI 3.16–8.43, p < 0.001). Conclusion: There is a significant lack of knowledge about creatinine and its use in checkups. The results indicate that greater efforts are needed from healthcare professionals to raise awareness on the role of serum creatinine. Introduction asymptomatic tests proteinuria Objective Method Crosssectional Cross sectional SP, SP (SP Brazil, Brazil , Brazil) interviewed Results 113 3 27–52 2752 27 52 (27–52) 551 55 1 55.1 “creatinine 406 40 6 40.6 exam 196 19 19.6 I dont don t know 71.6%, 716 71.6% 71 (71.6%) 0.9% 09 0 9 (0.9% 7.8%. 78 7.8% . 7 8 (7.8%) 294 29 4 29.4 knows “knows does 0.05 005 05 education sex studentworker student worker student/worker analysis OR 5.16 516 5 16 95 316843 43 3.16–8.43 0.001. 0001 0.001 001 0.001) Conclusion checkups 11 27–5 275 2 (27–52 55. 40. 19. 71.6 (71.6% 0.9 (0.9 7.8 (7.8% 29. 0.0 00 5.1 51 31684 3.16–8.4 000 0.00 27– (27–5 71. (71.6 0. (0. 7. (7.8 5. 3168 3.16–8. (27– (71. (0 (7. 316 3.16–8 (27 (71 ( (7 31 3.16– (2 3.16 3.1 3.

RESUMO O manejo dos níveis de condutividade elétrica é um dos fundamentos necessários para se obter maior produção e melhor qualidade de hortaliças. O objetivo deste trabalho foi avaliar o efeito da condutividade elétrica da solução nutritiva na cultura da couve-rábano cultivada em sistema semi-hidropônico. O delineamento experimental foi em blocos casualizados com cinco tratamentos associados à condutividade elétrica da solução nutritiva (1,31; 1,71; 2,37; 2,98 e 3,75 dS m-1) e quatro repetições. As plantas foram colhidas 78 dias após o transplante e avaliadas quanto ao crescimento, produção e qualidade pós-colheita. Solução nutritiva com condutividade elétrica (CE) de 1,31 dS m-1 proporciona maior produção de couve-rábano roxa. O aumento da CE reduziu linearmente a maioria das variáveis analisadas (área foliar, massa fresca de folhas, bulbos e parte aérea, massa seca de folhas e totais, volume e firmeza de bulbos), com perdas mais expressivas para as variáveis massa fresca de bulbos (50,54%) e volume de bulbos (57,37%). A utilização de solução nutritiva com CE entre 2,3 e 2,9 dS m-1 aumentou o teor de vitamina C e a acidez titulável. hortaliças couverábano couve rábano semihidropônico. semihidropônico semi hidropônico. hidropônico semi-hidropônico 131 1 31 (1,31 1,71 171 71 2,37 237 2 37 298 98 375 3 75 3,7 m1 m repetições 7 crescimento póscolheita. póscolheita pós colheita. colheita pós-colheita (CE 1,3 m- roxa área foliar aérea totais bulbos, , bulbos) 50,54% 5054 50 54 (50,54% 57,37%. 5737 57,37% . 57 (57,37%) 23 2, 29 9 titulável 13 (1,3 1,7 17 3, 1, 50,54 505 5 (50,54 573 57,37 (57,37% (1, 50,5 (50,5 57,3 (57,37 (1 50, (50, 57, (57,3 ( (50 (57, (5 (57

ABSTRACT Managing electrical conductivity levels is one of the fundamentals required to obtain greater production and better quality of vegetables. The aim of this study was to evaluate the effect of electrical conductivity of the nutrient solution on the kohlrabi crop cultivated in semi-hydroponic system. A randomized block design was used with five treatments associated with the electrical conductivity of the nutrient solution (1.31, 1.71, 2.37, 2.98, and 3.75 dS m-1) and four replications. The plants were harvested 78 days after transplanting and evaluated for growth, production, and postharvest quality. Nutrient solution with electrical conductivity (EC) of 1.31 dS m-1 promotes greater production of purple kohlrabi. The increase in EC reduced linearly most of the analyzed variables (leaf area, fresh mass of leaves, bulb and shoot, dry mass of leaves and total, bulb volume and firmness), with more significant losses for the bulb fresh mass (50.54%) and bulb volume (57.37%) variables. The use of nutrient solution with EC between 2.3 and 2.9 dS m-1 increased the vitamin C content and the titratable acidity. vegetables semihydroponic semi hydroponic system 1.31, 131 1 31 (1.31 171 71 1.71 237 2 37 2.37 298 98 2.98 375 3 75 3.7 m1 m replications 7 growth (EC 1.3 m- leaf area shoot total firmness, firmness , firmness) 50.54% 5054 50 54 (50.54% 57.37% 5737 57 (57.37% 23 2. 29 9 acidity 13 (1.3 17 1.7 3. 1. 50.54 505 5 (50.54 57.37 573 (57.37 (1. 50.5 (50.5 57.3 (57.3 (1 50. (50. 57. (57. ( (50 (57 (5

Stemodin (1) is a diterpene produced by Stemodia maritima L. (Scrophulariaceae) and shows antiviral and tumor cell proliferation inhibitory activities. Chemical modifications of 1 were performed to produce the known stemodinone (2), two new stemodinone oximes, Z (3) and E (4), and seven new oxime esters derivatives (5, 6, 7, 8, 9, 10, and 11). All derivatives were evaluated for their cytotoxic activity in HL60 (promyelocytic leukemia), SNB-19 (astrocytoma), HCT-116 (colon carcinoma), and PC3 (prostate) human cancer cell lines, and L929 (healthy murine cells). Oximes 3 and 4 showed lower cytotoxic values than 1 against all cancer cell lines tested. However, esters 9 and 10 exhibited cell growth inhibition percentages higher than 1 against PC3, SNB-19, and HCT-116 cancer cell lines, ranging from 62.96 to 94.27%. Many of these values exceed that presented by doxorubicin (66.05-86.87%). All compounds showed no cytotoxic effect on healthy cells at the tested concentration of 25 µg mL-1. (1 L Scrophulariaceae (Scrophulariaceae activities 2, 2 , (2) oximes (3 4, (4) 5, 5 (5 6 7 8 11. 11 . 11) HL HL6 promyelocytic leukemia, leukemia leukemia) SNB19 SNB 19 SNB-1 astrocytoma, astrocytoma (astrocytoma) HCT116 HCT 116 HCT-11 colon carcinoma, carcinoma carcinoma) PC prostate (prostate L92 cells. cells) However SNB19, 19, 6296 62 96 62.9 9427 94 27 94.27% 66.0586.87%. 66058687 66.05 86.87% 66 05 86 87 (66.05-86.87%) mL1. mL1 mL 1. mL-1 ( (2 (4 SNB1 SNB- (astrocytoma HCT11 HCT-1 L9 629 62. 942 94.27 0586 66.0586.87% 6605868 6605 66.0 8687 86.87 0 (66.05-86.87% mL- HCT1 HCT- 94.2 058 66.0586.87 660586 660 66. 868 86.8 (66.05-86.87 94. 66.0586.8 66058 86. (66.05-86.8 66.0586. (66.05-86. 66.0586 (66.05-86 66.058 (66.05-8 (66.05- (66.05 (66.0 (66. (66 (6

ABSTRACT BACKGROUND: Contamination of the breathing circuit and medication preparation surface of an anesthesia machine can increase the risk of cross-infection. OBJECTIVE: To evaluate the contamination of the anesthetic medication preparation surface, respiratory circuits, and devices used in general anesthesia with assisted mechanical ventilation. DESIGN AND SETTING: Cross-sectional, quantitative study conducted at the surgical center of a philanthropic hospital, of medium complexity located in the municipality of Três Lagoas, in the eastern region of the State of Mato Grosso do Sul. METHODS: Eighty-two microbiological samples were collected from the breathing circuits. After repeating the samples in different culture media, 328 analyses were performed. RESULTS: A higher occurrence of E. coli, Enterobacter spp., Pseudomonas spp., Staphylococcus aureus, and Streptococcus pneumoniae (P < 0.001) were observed. Variations were observed depending on the culture medium and sample collection site. CONCLUSION: The study findings underscore the inadequate disinfection of the inspiratory and expiratory branches, highlighting the importance of stringent cleaning and disinfection of high-touch surfaces. BACKGROUND crossinfection. crossinfection cross infection. infection cross-infection OBJECTIVE circuits ventilation SETTING Crosssectional, Crosssectional Cross sectional, sectional Cross-sectional hospital Lagoas Sul METHODS Eightytwo Eighty two media 32 performed RESULTS E coli spp spp. aureus P 0.001 0001 0 001 site CONCLUSION branches hightouch high touch surfaces 3 0.00 000 00 0.0 0.

Coronavirus disease (COVID-19) pandemic affected several business segments due to the lockdown period, including the illicit drug market. As Brazil usually imports new psychoactive substances (NPS), it was expected that their traffic would change during the pandemic. This study aimed to characterize NPS infused in pieces of paper seized inside prisons in the State of Rio de Janeiro (RJ), Brazil, during the COVID-19 pandemic by gas chromatography coupled with mass spectrometry, attenuated total reflectance-Fourier transform infrared spectroscopy, and high-resolution mass spectrometry. All samples were identified as synthetic cannabinoid receptor agonists, with indole-indazole structures. It was the first record of methyl (2S)-3,3-dimethyl-2-(1-(pent-4-en-1-yl)-1H-indazole-3-carboxamido) butanoate (MDMB-4en-PINACA), N-[(2S)-1-amino-3,3-dimethyl-1-oxobutan-2-yl]-1-butylindazole-3-carboxamide (ADB-BUTINACA), and N-(1-adamantyl)-1-(4-fluorobutyl)indazole-3-carboxamide (4F-ABUTINACA) in RJ, demonstrating that new NPS still arrived despite the lockdown. The integration of the data obtained via this study enabled the unequivocal identification of NPS even in the absence of standard reference materials, which is a common drawback faced by forensic laboratories in the analysis of NPS. COVID19 COVID 19 (COVID-19 period market NPS, , (NPS) RJ (RJ) COVID-1 spectrometry reflectanceFourier reflectance Fourier spectroscopy highresolution high resolution agonists indoleindazole indole indazole structures 2S3,3dimethyl21pent4en1yl1Hindazole3carboxamido 2S33dimethyl21pent4en1yl1Hindazole3carboxamido SdimethylpentenylHindazolecarboxamido 2S 3,3 dimethyl 2 1 pent 4 en yl 1H 3 carboxamido S H (2S)-3,3-dimethyl-2-(1-(pent-4-en-1-yl)-1H-indazole-3-carboxamido MDMB4enPINACA, MDMB4enPINACA MDMBenPINACA MDMB 4en PINACA (MDMB-4en-PINACA) N2S1amino3,3dimethyl1oxobutan2yl1butylindazole3carboxamide N2S1amino33dimethyl1oxobutan2yl1butylindazole3carboxamide NSaminodimethyloxobutanylbutylindazolecarboxamide N amino oxobutan butylindazole carboxamide ADBBUTINACA, ADBBUTINACA ADB BUTINACA (ADB-BUTINACA) N1adamantyl14fluorobutylindazole3carboxamide Nadamantylfluorobutylindazolecarboxamide adamantyl fluorobutyl 4FABUTINACA FABUTINACA 4F ABUTINACA F (4F-ABUTINACA materials COVID1 (COVID-1 (NPS (RJ COVID- 2S3 3dimethyl21pent4en1yl1Hindazole3carboxamido Hindazole 33 3, enPINACA (MDMB-4en-PINACA N2S1amino3 3dimethyl1oxobutan2yl1butylindazole3carboxamide (ADB-BUTINACA fluorobutylindazole (COVID- dimethylpentenylHindazolecarboxamido NSamino N2S1amino dimethyloxobutanylbutylindazolecarboxamide (COVID

Abstract Objectives To assess the hearing thresholds in acute otitis media, otitis media with effusion and chronic otitis media (non-suppurative, non-cholesteatomatous suppurative and cholesteatomatous) and to compare the hearing outcomes with non-diseased ears (in bilateral cases) or contralateral healthy ears (in unilateral cases), since hearing loss is the most frequent sequel of otitis media and there is no previous study comparing the audiometric thresholds among the different forms of otitis media. Methods Cross sectional, controlled study. We performed conventional audiometry (500-8000 Hz) and tympanometry in patients with otitis media and healthy individuals (control group). Hearing loss was considered when the hearing thresholds were > 25 dBHL. Results Of the 112 patients diagnosed with otitis media (151 ears), 48 were men (42.86%) and 64 were women (57.14%). The average age was 42.72 years. Of those, 25 (22.32%) were diagnosed as AOM, 15 (13.39%) were diagnosed with OME and the remaining 72 (63.28%) were diagnosed with COM (non-suppurative COM, n = 31; suppurative COM, n = 18; cholesteatomatous COM, n = 23). As compared with controls, all forms of otitis media had significantly higher bone-conduction thresholds (500-4000 Hz). Conductive hearing loss was the most frequent type of hearing loss (58.94%). However, the number of patients with mixed hearing loss was also relevant (39.07%). We noted that the presence of sensorioneural component occurred more frequently in 1) Higher frequencies; and 2) In groups of otitis media that were more active or severe in the inflammatory/infective standpoint (AOM, suppurative COM and cholesteatomatous COM). Conclusion All types of otitis media, even those with infrequent episodes of inflammation and otorrhea, had worse bone conduction thresholds as compared with nondiseased ears (p < 0.01). We observed worse hearing outcomes in ears with recurrent episodes of otorrhea and in ears with AOM, especially in high frequencies. nonsuppurative, nonsuppurative non suppurative, noncholesteatomatous diseased cases cases, , sectional 5008000 500 8000 (500-800 Hz control group. group . group) 2 dBHL 11 151 (15 ears, ears) 4 42.86% 4286 42 86 (42.86% 6 57.14%. 5714 57.14% 57 14 (57.14%) 4272 42.7 years 22.32% 2232 22 32 (22.32% AOM 1 13.39% 1339 13 39 (13.39% 7 63.28% 6328 63 28 (63.28% 31 18 23. 23 23) controls boneconduction 5004000 4000 (500-400 Hz. 58.94%. 5894 58.94% 58 94 (58.94%) However 39.07%. 3907 39.07% 07 (39.07%) frequencies inflammatoryinfective inflammatory infective (AOM COM. COM) p 0.01. 001 0.01 0 01 0.01) 500800 50 800 (500-80 (1 42.86 428 8 (42.86 571 57.14 5 (57.14% 427 42. 22.32 223 3 (22.32 13.39 133 (13.39 63.28 632 (63.28 500400 400 (500-40 589 58.94 9 (58.94% 390 39.07 (39.07% 00 0.0 50080 80 (500-8 ( 42.8 (42.8 57.1 (57.14 22.3 (22.3 13.3 (13.3 63.2 (63.2 50040 40 (500-4 58.9 (58.94 39.0 (39.07 0. 5008 (500- (42. 57. (57.1 22. (22. 13. (13. 63. (63. 5004 58. (58.9 39. (39.0 (500 (42 (57. (22 (13 (63 (58. (39. (50 (4 (57 (2 (6 (58 (39 (5 (3

In this study, we examine the effects of solvent media on the structural and optical behaviors of two isomers of thiazine derivatives: rac-2-(4-nitrophenyl)-3-phenyl-2,3,5,6-tetrahydro-4H 1,3 thiazin-4-one and (2S)-2-(3-nitrophenyl)-3-phenyl-2,3,5,6-tetrahydro-4H-1,3-thiazin-4 one. The solvent effects were modeled using the polarizable continuum model through density functional theory. Electrical parameters for rac-2-(4-nitrophenyl)-3-phenyl-2,3,5,6-tetrahydro-4H 1,3-thiazin-4-one and (2S)-2-(3-nitrophenyl)-3-phenyl-2,3,5,6-tetrahydro-4H-1,3-thiazin-4 one were determined using the density functional theory at the CAM-B3LYP/6-311+G(d) level. We studied the influence of isomer structures in various solvent media on the Hyper-Rayleigh-Scattering first hyperpolarizability, considering both static and dynamic scenarios. This research particularly emphasizes the implications of relocating the NO2 group from the meta-position (2S)-2-(3-nitrophenyl)-3-phenyl-2,3,5,6-tetrahydro-4H-1,3-thiazin-4-one to the para-position rac-2-(4-nitrophenyl)-3-phenyl-2,3,5,6-tetrahydro-4H-1,3-thiazin-4-one on molecular geometries, linear and nonlinear optical parameters, and gap energies across different solvent media. Bond dissociation energy calculations for hydrogen atoms and all other single acyclic bonds were performed for both derivatives to assess degradation and autoxidation properties. Additional insights from non-bonding orbitals, molecular electrostatic surface potential, Fukui calculations, electron localization function, and localized orbital locator are detailed. study rac24nitrophenyl3phenyl2,3,5,6tetrahydro4H rac24nitrophenyl3phenyl2356tetrahydro4H racnitrophenylphenyltetrahydroH rac 2 4 nitrophenyl 3 phenyl 2,3,5,6 tetrahydro 4H 5 6 H 13 1 1, thiazin4one thiazinone thiazin 2S23nitrophenyl3phenyl2,3,5,6tetrahydro4H1,3thiazin4 2S23nitrophenyl3phenyl2356tetrahydro4H13thiazin4 SnitrophenylphenyltetrahydroHthiazin 2S S (2S)-2-(3-nitrophenyl)-3-phenyl-2,3,5,6-tetrahydro-4H-1,3-thiazin- 1,3thiazin4one 13thiazin4one CAMB3LYP/6311+Gd CAMB3LYP6311Gd CAMBLYPGd CAM B3LYP/6 311+G d B3LYP 311 G B LYP CAM-B3LYP/6-311+G(d level HyperRayleighScattering Hyper Rayleigh Scattering hyperpolarizability scenarios NO metaposition meta position 2S23nitrophenyl3phenyl2,3,5,6tetrahydro4H1,3thiazin4one 2S23nitrophenyl3phenyl2356tetrahydro4H13thiazin4one SnitrophenylphenyltetrahydroHthiazinone paraposition para rac24nitrophenyl3phenyl2,3,5,6tetrahydro4H1,3thiazin4one rac24nitrophenyl3phenyl2356tetrahydro4H13thiazin4one racnitrophenylphenyltetrahydroHthiazinone geometries properties nonbonding non bonding orbitals potential function detailed rac24nitrophenyl3phenyl2 6tetrahydro4H 2356 2,3,5, 2S23nitrophenyl3phenyl2 6tetrahydro4H1 3thiazin4 2S23nitrophenyl3phenyl2,3,5,6tetrahydro4H1,3thiazin 2S23nitrophenyl3phenyl2356tetrahydro4H13thiazin (2S)-2-(3-nitrophenyl)-3-phenyl-2,3,5,6-tetrahydro-4H-1,3-thiazin 3thiazin4one CAMB3LYP 6311 Gd CAMB B3LYP6 BLYP B3LYP/ 311G 31 racnitrophenylphenyl rac24nitrophenyl3phenyl tetrahydroH 235 2,3,5 Snitrophenylphenyl 2S23nitrophenyl3phenyl 3thiazin CAMBLYP 631 23 2,3, 63 2,3 2,

The interactive profile between four 1,4-naphthoquinone derivatives (1-4) and human serum albumin (HSA) was studied by spectroscopic techniques and in silico calculations. The bimolecular quenching rate constant (kq ca. 1012 L mol-1 s-1) and the time-resolved fluorescence decays indicated a static fluorescence quenching mechanism. Thus, there is a spontaneous ground-state association, and based on both Stern-Volmer, modified Stern-Volmer, and van’t Hoff approaches, the association is moderate mainly driven by hydrophobic forces. The circular dichroism (CD) analysis indicated that until the proportion albumin:compound of 1:8 there is a weak perturbation on the structural content of albumin, while molecular docking results suggested subdomain IIA (site I), a positive electrostatic pocket, as the main binding site. Overall, even though the hydrogen atom replacement by methyl, fluorine, or chlorine atoms in the para position of the aromatic ring in the benzo[g]chromene-5,10-dione moiety changes the lipophilicity, it does not change the binding profile to HSA. 1,4naphthoquinone 14naphthoquinone naphthoquinone 1,4 1 4 14 (1-4 HSA (HSA calculations kq ca 101 mol1 mol mol- s1 s s-1 timeresolved time resolved mechanism Thus groundstate ground state SternVolmer, SternVolmer Stern Volmer, Volmer Stern-Volmer vant van t approaches forces CD (CD albumincompound compound 18 8 1: site I, I , I) pocket Overall methyl fluorine benzogchromene5,10dione benzogchromene510dione benzogchromenedione benzo g chromene 5,10 dione 5 10 lipophilicity 4naphthoquinone 1, (1- s- benzogchromene5 10dione benzogchromene 510 5,1 (1 51 5, (

RESUMO: Efeitos fitotóxicos induzidos em plântulas de soja devido à exposição a diferentes pesticidas podem resultar em encurtamento e engrossamento de hipocótilo, atrofia na raiz principal e redução do desenvolvimento ou ausência de raízes secundárias. Este trabalho buscou validar o sistema de análise de imagens Vigor-S para caracterizar os efeitos fitotóxicos em plântulas de soja, provenientes do tratamento de sementes com diferentes produtos. Sementes de soja da cultivar BRS 284 foram submetidas a três tratamentos: fungicida ciproconazol, que resulta em encurtamento do hipocótilo; herbicida glifosato, que resulta em redução no desenvolvimento do sistema radicular; e controle (sem tratamento). O delineamento experimental consistiu em blocos ao acaso com seis repetições. A qualidade fisiológica das sementes foi avaliada por meio de testes de germinação, e o comprimento das plântulas foi medido tanto pelo método tradicional quanto pelo sistema de análise de imagens Vigor-S. Observou-se uma correlação positiva significativa ao comparar os parâmetros de comprimento das plântulas entre os métodos tradicional e Vigor-S. Concluiu-se que plântulas que apresentam sintomas de fitotoxicidade causados pela exposição ao herbicida glifosato e ao fungicida ciproconazol apresentam comprimento de plântulas reduzido. O sistema Vigor-S efetivamente detecta ambos os tipos de fitotoxicidade em plântulas de soja. RESUMO hipocótilo secundárias VigorS Vigor S produtos 28 tratamentos radicular sem tratamento. . tratamento) repetições germinação VigorS. S. Observouse Observou se Concluiuse Concluiu reduzido 2

ABSTRACT: Phytotoxic effects induced in soybean seedlings by exposure to various pesticides can lead to shortened and thickened hypocotyls, taproot atrophy, and reduced development or absence of secondary roots. This study aimed to validate the Vigor-S image analysis system for characterizing phytotoxic effects on soybean seedlings resulting from seed treatment using different products. Soybean seeds of cultivar BRS 284 received three seed treatments: cyproconazole fungicide, which causes hypocotyl shortening; glyphosate herbicide, which results in reduced root system; and control (without treatment). The experiment used a randomized complete block design with six replications. Seed physiological quality was assessed by the germination test, seedling length by the traditional method and also determined by the Vigor-S system method. There was a significant positive correlation when comparing seedling length parameters between the conventional and Vigor-S methods. It was concluded that seedlings exhibiting phytotoxicity symptoms from exposure to glyphosate herbicide and cyproconazole fungicide had reduced seedling length. The Vigor-S system effectively detects both types of phytotoxicity in soybean seedlings, which could have implications for agricultural practices. ABSTRACT hypocotyls atrophy roots VigorS Vigor S products 28 treatments shortening without treatment. . treatment) replications test methods practices 2

ABSTRACT Objective: To analyze the number of hospitalizations, the length of hospital stay, and mortality due to asthma, as well as the costs to the Unified Health Care System in Brazil between 2008 and 2021. Methods: This was a cross-sectional epidemiological study using data from the Information Technology Department of the Brazilian Unified Health Care System. Proportional hospitalization and death rates were estimated per 100,000 population by age, microregion, and year. Results: The number of hospitalizations and deaths due to asthma decreased from 2008 to 2021 (205,392 vs. 55,009 and 822 vs. 327, respectively). In addition, a between-sex difference was observed in asthma-related hospitalizations in 2008, and more men were hospitalized in 2021 (51.8%). Asthma mortality rates were similar for both sexes (50.0% each) in 2008, and a slight increase was observed in women’s deaths in 2021 (52.9%). Even so, approximately one death/day and more than 55,000 hospitalizations were observed yearly, with a mean length of hospital stay of three days. Additionally, the Southeast region allocated more financial resources to asthma-related hospitalizations. Conclusions: Our results showed that the number of deaths and hospitalizations due to asthma substantially declined during the study period. Objective 200 Methods crosssectional cross sectional 100000 100 000 100,00 age microregion year Results 202 205,392 205392 205 392 (205,39 vs 55009 55 009 55,00 82 327 respectively. respectively . respectively) addition betweensex sex asthmarelated related 51.8%. 518 51.8% 51 8 (51.8%) 50.0% 500 50 0 (50.0 each womens women s 52.9%. 529 52.9% 52 9 (52.9%) so deathday day 55000 yearly days Additionally Conclusions period 20 10000 10 00 100,0 205,39 20539 39 (205,3 5500 5 55,0 32 51.8 (51.8% 50.0 (50. 52.9 (52.9% 2 1000 1 100, 205,3 2053 3 (205, 550 55, 51. (51.8 50. (50 52. (52.9 205, (205 (51. (5 (52. (20 (51 ( (52 (2

RESUMO O câncer de pulmão (CP) é uma das neoplasias mais comuns e letais no Brasil, e apenas 15% dos pacientes são diagnosticados nos estágios iniciais. O tabagismo persiste como o responsável por mais de 85% de todos os casos. O rastreamento do CP (RCP) por meio da TC de baixa dosagem de radiação (TCBD) reduz a mortalidade do CP em 20%, e, quando combinado com a cessação do tabagismo, essa redução chega a 38%. Na última década, diversos países adotaram o RCP como recomendação de saúde populacional. No Brasil, embora ainda incipiente, a discussão sobre o tema é cada vez mais ampla e necessária. Com o intuito de aumentar o conhecimento e estimular o debate sobre o RCP, a Sociedade Brasileira de Cirurgia Torácica, a Sociedade Brasileira de Pneumologia e Tisiologia e o Colégio Brasileiro de Radiologia e Diagnóstico por Imagem constituíram um painel de especialistas para elaborar as recomendações para o RCP. As recomendações aqui apresentadas foram baseadas em revisão narrativa da literatura, com ênfase em grandes estudos populacionais, em revisões sistemáticas e em recomendações de diretrizes internacionais, sendo construídas após ampla discussão pelo grupo de especialistas. Os temas revisados foram os seguintes: porque rastrear, considerações gerais sobre tabagismo, epidemiologia do CP, critérios de elegibilidade, achados incidentais, lesões granulomatosas, modelos probabilísticos, requisitos mínimos da TCBD, aquisições volumétricas, riscos do rastreamento, estrutura mínima e papel da equipe multidisciplinar, conduta segundo o Lung CT Screening Reporting and Data System (Lung-RADS), custos vs. benefícios e perspectivas do rastreamento. (CP Brasil 15 iniciais 85 casos (RCP TCBD (TCBD 20 20% 38 38% década populacional incipiente necessária Torácica literatura populacionais internacionais seguintes rastrear elegibilidade incidentais granulomatosas probabilísticos volumétricas multidisciplinar LungRADS, LungRADS RADS , (Lung-RADS) vs 1 8 2 3 (Lung-RADS

ABSTRACT Although lung cancer (LC) is one of the most common and lethal tumors, only 15% of patients are diagnosed at an early stage. Smoking is still responsible for more than 85% of cases. Lung cancer screening (LCS) with low-dose CT (LDCT) reduces LC-related mortality by 20%, and that reduction reaches 38% when LCS by LDCT is combined with smoking cessation. In the last decade, a number of countries have adopted population-based LCS as a public health recommendation. Albeit still incipient, discussion on this topic in Brazil is becoming increasingly broad and necessary. With the aim of increasing knowledge and stimulating debate on LCS, the Brazilian Society of Thoracic Surgery, the Brazilian Thoracic Association, and the Brazilian College of Radiology and Diagnostic Imaging convened a panel of experts to prepare recommendations for LCS in Brazil. The recommendations presented here were based on a narrative review of the literature, with an emphasis on large population-based studies, systematic reviews, and the recommendations of international guidelines, and were developed after extensive discussion by the panel of experts. The following topics were reviewed: reasons for screening; general considerations about smoking; epidemiology of LC; eligibility criteria; incidental findings; granulomatous lesions; probabilistic models; minimum requirements for LDCT; volumetric acquisition; risks of screening; minimum structure and role of the multidisciplinary team; practice according to the Lung CT Screening Reporting and Data System; costs versus benefits of screening; and future perspectives for LCS. LC (LC tumors 15 stage 85 cases (LCS lowdose low dose (LDCT LCrelated related 20 20% 38 cessation decade populationbased population recommendation incipient necessary Surgery Association literature studies reviews guidelines reviewed criteria findings lesions models acquisition team System 1 8 2 3

ABSTRACT Objective: Talc pleurodesis is a widely used treatment option for malignant pleural effusion (MPE). However, the optimal form of administration remains controversial. Thus, we performed a systematic review and meta-analysis to assess the effectiveness of talc slurry (TS) in comparison with thoracoscopic talc insufflation/poudrage (TTI) for MPE treatment. Methods: We searched PubMed, EMBASE, and Cochrane Library databases for studies that compared TS with TTI in patients with MPE. We used a random-effects model with a 95% CI to pool the data. Heterogeneity was assessed with I2 statistics. Results: We included eight studies involving 1,163 patients, 584 of whom (50.21%) underwent TS. Pleurodesis failure rates were similar between the procedures (OR = 1.07; 95% CI: 0.56-2.06; p = 0.83; I2 = 62%); and 68% of patients (95% CI: 0.31-1.47; p = 0.33; I2 = 58%) had postoperative complications, which were lower in patients in the TS group than in the TTI group. In a subgroup analysis considering only randomized clinical trials, the failure rate was significantly lower in the TS treatment group (OR = 0.62; 95% CI: 0.42-0.90; p = 0.01; I2 = 0%). Similarly, dyspnea was less common in the TS group (OR = 0.74; 95% CI: 0.41-1.34; p = 0.32; I2 = 55%). Adverse effects were reported in 86 patients, and no significant difference was seen between the TS and TTI groups: empyema (OR = 1.43; 95% CI: 0.36-5.64; p = 0.86; I2 = 0%), pain (OR = 1.22 (95% CI: 0.67-2.21; p = 0.51; I2 = 38%), and pneumonia (OR = 1.15; 95% CI: 0.30-4.46; p = 0.86; I2 = 27%). Conclusions: Our findings suggest that TS is an effective treatment for MPE, with no significant increase in adverse events. Results suggest equivalent efficacy and safety for both procedures. Objective . (MPE) However controversial Thus metaanalysis meta (TS insufflationpoudrage insufflation poudrage (TTI Methods PubMed EMBASE randomeffects random 95 data I statistics 1163 1 163 1,16 58 50.21% 5021 50 21 (50.21% OR 1.07 107 07 0.562.06 056206 0.56 2.06 0 56 2 06 0.56-2.06 0.83 083 83 62% 62 62%) 68 (95 0.311.47 031147 0.31 1.47 31 47 0.31-1.47 0.33 033 33 58% complications trials 0.62 062 0.420.90 042090 0.42 0.90 42 90 0.42-0.90 0.01 001 01 0%. 0% 0%) Similarly 0.74 074 74 0.411.34 041134 0.41 1.34 41 34 0.41-1.34 0.32 032 32 55%. 55 55% 55%) 8 groups 1.43 143 43 0.365.64 036564 0.36 5.64 36 5 64 0.36-5.64 0.86 086 0%, , 122 22 1.2 0.672.21 067221 0.67 2.21 67 0.67-2.21 0.51 051 51 38%, 38 38% 38%) 1.15 115 15 0.304.46 030446 0.30 4.46 30 4 46 0.30-4.46 27%. 27 27% 27%) Conclusions events (MPE 9 116 16 1,1 50.21 502 (50.21 1.0 10 562 0.562.0 05620 056 0.5 206 2.0 0.56-2.0 0.8 08 6 (9 311 0.311.4 03114 031 0.3 147 1.4 3 0.31-1.4 03 0.6 420 0.420.9 04209 042 0.4 090 0.9 0.42-0.9 0.0 00 0.7 7 411 0.411.3 04113 041 134 1.3 0.41-1.3 14 365 0.365.6 03656 036 564 5.6 0.36-5.6 12 1. 672 0.672.2 06722 067 221 2.2 0.67-2.2 05 1.1 11 304 0.304.4 03044 030 446 4.4 0.30-4.4 1, 50.2 (50.2 0.562. 0562 0. 20 2. 0.56-2. ( 0.311. 0311 0.31-1. 0.420. 0420 04 09 0.42-0. 0.411. 0411 13 0.41-1. 0.365. 0365 5. 0.36-5. 0.672. 0672 0.67-2. 0.304. 0304 44 4. 0.30-4. 50. (50. 0.562 0.56-2 0.311 0.31-1 0.420 0.42-0 0.411 0.41-1 0.365 0.36-5 0.672 0.67-2 0.304 0.30-4 (50 0.56- 0.31- 0.42- 0.41- 0.36- 0.67- 0.30- (5

RESUMO A água é um recurso essencial na agricultura e a sua utilização eficiente é fundamental para garantir a sustentabilidade do sector. A utilização de tecnologias mais racionais e sustentáveis é uma estratégia importante para otimizar o uso da água na agricultura. Diante do exposto, o objetivo deste estudo foi avaliar o efeito de diferentes lâminas de irrigação nas variáveis químicas e físicas de melão, e produtividade, em plantas cultivadas com ou sem aplicação de hidrogel. O experimento foi conduzido em delineamento de blocos casualizados com dez tratamentos e quatro blocos. Os tratamentos foram dispostos em esquema fatorial 5 × 2, referente a cinco lâminas de irrigação (50, 75, 100, 125 e 150% da evapotranspiração da cultura - ETc), e com ou sem aplicação de hidrogel (4 g L-1). O sistema de irrigação utilizado foi o gotejamento. As lâminas de irrigação influenciaram positivamente as variáveis avaliadas, sendo a maior produtividade (39.075,69 kg ha-1) obtida com a maior lâmina de água de 394 mm (150% ETc), enquanto o uso de hidrogel reduziu a cavidade interna do fruto. As características físicas dos frutos (espessura da polpa e cavidade interna) apresentaram incrementos lineares positivos dentro do intervalo de lâminas de irrigação avaliadas. A condutividade elétrica dos frutos apresentou diminuição linear com o aumento das lâminas de irrigação, enquanto o pH se comportou como equação polinomial quadrática. O uso de hidrogel não foi suficiente para mitigar os efeitos negativos das lâminas deficitárias. Porém, o aumento das lâminas de água melhorou a qualidade e a produtividade de melão. sector exposto melão 2 50, 50 (50 75 100 12 150 ETc, ETc , ETc) 4 ( L1. L1 L 1 . L-1) gotejamento avaliadas 39.075,69 3907569 39 075 69 (39.075,6 ha1 ha ha-1 (150 fruto espessura quadrática deficitárias Porém (5 7 10 15 L-1 39.075,6 390756 3 07 6 (39.075, ha- (15 L- 39.075, 39075 0 (39.075 (1 39.075 3907 (39.07 39.07 390 (39.0 39.0 (39. 39. (39 (3

ABSTRACT Water is an essential resource in agriculture, and its efficient use is fundamental to ensuring the sustainability of the sector. The use of more rational and sustainable technologies is an important strategy for optimizing the use of water in agriculture. In view of the above, the aim of this study was to evaluate the effect of different irrigation depths on both chemical and physical variables of melon fruits, and yield, in plants grown with or without hydrogel application. The experiment was conducted in a randomized block design with ten treatments and four blocks. The treatments were arranged in a 5 × 2 factorial scheme, referring to five irrigation depths (50, 75, 100, 125, and 150% of crop evapotranspiration - ETc), and with or without hydrogel application (4 g L-1). The irrigation system used was drip irrigation. Irrigation depths positively influenced the variables evaluated, with the highest yield (39,075.69 kg ha-1) obtained with the highest water depth of 394 mm (150% ETc), while the use of hydrogel reduced the internal cavity of the fruit. The fruit physical characteristics (pulp thickness and internal cavity) showed positive linear increments within the interval of irrigation depth evaluated. Fruit electrical conductivity showed a linear decrease as the irrigation depths increased, while the pH was described by a quadratic polynomial equation. The use of hydrogel was not enough to mitigate the negative effects of deficit irrigation. However, the increase in irrigation depths improved the quality and yield of melon fruit. agriculture sector above fruits blocks scheme 50, 50 (50 75 100 125 150 ETc, ETc , ETc) 4 ( L1. L1 L 1 . L-1) evaluated 39,075.69 3907569 39 075 69 (39,075.6 ha1 ha ha-1 (150 pulp increased equation However (5 7 10 12 15 L-1 39,075.6 390756 3 07 6 (39,075. ha- (15 L- 39,075. 39075 0 (39,075 (1 39,075 3907 (39,07 39,07 390 (39,0 39,0 (39, 39, (39 (3

Abstract Background: Loxoscelism refers to a set of clinical manifestations caused by the bite of spiders from the Loxosceles genus. The classic clinical symptoms are characterized by an intense inflammatory reaction at the bite site followed by local necrosis and can be classified as cutaneous loxoscelism. This cutaneous form presents difficult healing, and the proposed treatments are not specific or effective. This study aimed to evaluate the protective effect of mesenchymal stromal cells-derived secretome on dermonecrosis induced by Loxosceles intermedia spider venom in rabbits. Methods: Sixteen rabbits were distributed into four groups (n = 4). Except for group 1 (G1), which received only PBS, the other three groups (G2, G3, and G4) were initially challenged with 10 μg of L. intermedia venom, diluted in 100 μL of NaCl 0.9%, by intradermic injection in the interscapular region. Thirty minutes after the challenge all groups were treated with secretome, except for group 2. Group 1 (G1-control group) received intradermal injection (ID) of 60 μg of secretome in 0.15 M PBS; Group 2 (G2) received 0.9% NaCl via ID; Group 3 (G3) received 60 μg of secretome, via ID and Group 4 (G4), received 60 μg of secretome by intravenous route. Rabbits were evaluated daily and after 15 days were euthanized, necropsied and skin samples around the necrotic lesions were collected for histological analysis. Results: Rabbits of G1 did not present edema, erythema, hemorrhagic halo, or necrosis. In animals from G2, G3, and G4, edema appeared after 6h. However, minor edema was observed in the animals of G2 and G3. Hemorrhagic halo was observed in animals, six hours and three days after, on G2, G3, and G4. Macroscopically, in G4, only one animal out of four had a lesion that evolved into a dermonecrotic wound. No changes were observed in the skin of the animals of G1, by microscopic evaluation. All animals challenged with L. intermedia venom showed similar alterations, such as necrosis and heterophilic infiltration. However, animals from G4 showed fibroblast activation, early development of connective tissue, neovascularization, and tissue re-epithelialization, indicating a more prominent healing process. Conclusion: These results suggest that secretome from mesenchymal stromal cells cultured in a xeno-free and human component-free culture media can be promising to treat dermonecrosis caused after Loxosceles spiders bite envenoming. Background genus loxoscelism effective cellsderived derived Methods n 4. . 4) G , (G1) PBS (G2 G3 L 09 0 9 region G1control Gcontrol control (ID 6 015 0.1 0.9 (G3 (G4) route euthanized analysis Results erythema 6h h However Macroscopically wound evaluation alterations infiltration activation neovascularization reepithelialization, reepithelialization re epithelialization, epithelialization re-epithelialization process Conclusion xenofree xeno free componentfree component envenoming (G1 (G 01 0. (G4

Resumo Fundamento Não existem estudos randomizados comparando a manutenção do ritmo sinusal após ablação por cateter (AC) em relação ao tratamento com fármacos antiarrítmicos (AA) em pacientes idosos portadores fibrilação atrial (FA) paroxística. Objetivos Comparar os resultados clínicos do isolamento das veias pulmonares (VPs) com o cateter PVAC Gold de segunda geração com o uso de AA em idosos com FA paroxística sintomática, recorrente, apesar do uso de fármacos AA. Métodos Sessenta pacientes com FA paroxística ≥ 65 anos e sem cardiopatias estruturais foram randomizados para duas formas de tratamento: grupo 1: AC e grupo 2: AA. O desfecho primário foi a taxa livre de recorrência de FA após pelo menos um ano de seguimento. Os desfechos secundários foram: progressão para formas persistentes de FA, impacto na qualidade de vida (QVFA) e complicações. O nível de significância adotado na análise estatística foi de 5% (p<0,05). Resultados A taxa livre de recorrência de FA foi de 80% (10% com amiodarona) no grupo AC, após 1,3 procedimentos por paciente e de 65% no grupo AA (60% com amiodarona), (p = 0,119) num seguimento médio de 719 dias (Q1: 566; Q3: 730). A taxa livre de FA persistente foi de 83,4% no grupo AC e de 67,7% no grupo AA (p = 0,073). Ambas as estratégias apresentaram melhora no escore de QVFA durante o seguimento (p < 0,001), sem diferença entre os grupos. Embora sem repercussão clínica ou impacto no teste de avaliação intelectual, 25% dos pacientes do grupo PVAC apresentou sinais de embolização cerebral na RNM cerebral. Conclusões Ambas as estratégias para manutenção do ritmo sinusal promoveram melhora na qualidade de vida de pacientes idosos com FA sintomática, sem diferença estatística nos desfechos clínicos preconizados. Estudos adicionais usando tecnologias com melhor perfil de segurança são necessários para avaliar os benefícios da AC em pacientes idosos com FA. (AC (AA (FA VPs (VPs sintomática recorrente 6 1 2 (QVFA complicações 5 p<0,05. p005 p p<0,05 . 0 05 (p<0,05) 80 10% 10 (10 amiodarona 13 3 1, 60% 60 (60 amiodarona, , 0,119 0119 119 71 Q1 Q (Q1 566 Q3 730. 730 730) 834 83 4 83,4 677 67 7 67,7 0,073. 0073 0,073 073 0,073) 0,001, 0001 0,001 001 0,001) grupos intelectual 25 preconizados p00 p<0,0 (p<0,05 8 (1 (6 0,11 011 11 (Q 56 73 83, 67, 007 0,07 07 000 0,00 00 p0 p<0, (p<0,0 ( 0,1 01 0,0 p<0 (p<0, 0, p< (p<0 (p<

Abstract Background There are no randomized studies comparing the maintenance of sinus rhythm after catheter ablation (CA) concerning treatment with antiarrhythmic drugs (AA) in elderly patients with paroxysmal atrial fibrillation (AF). Objectives To compare the clinical results of pulmonary vein (PV) isolation with the second-generation PVAC Gold catheter against AA treatment in elderly people with recurrent symptomatic paroxysmal AF, refractory to at least one AA, and without structural heart disease. Methods Sixty patients with paroxysmal AF ≥ 65 years old were randomized to two forms of treatment: group 1: CA and group 2: AA drugs. The primary outcome was the AF recurrence-free rate after at least one year of follow-up. Secondary outcomes were: progression to persistent forms of AF, impact on quality of life (QOLF), and complications. The significance level adopted in the statistical analysis was 5% (p<0.05). Results The AF recurrence-free rate was 80% (10% with amiodarone) in the CA group, after 1.3 procedures per patient and 65% in the AA group (60% with amiodarone), (p = 0.119) in an average follow-up of 719 days (Q1: 566; Q3: 730). The persistent AF free rate was 83.4% in the AC group and 67.7% in the AA group (p = 0.073) Both strategies showed an improvement in the AFQoL score during follow-up (p < 0.001), with no difference between the groups. Although without clinical repercussions or impact on the intellectual assessment test, 25% of patients in the CA group showed signs of cerebral embolization on brain MRI. Conclusions Both strategies for maintaining sinus rhythm promoted an improvement in the quality of life of elderly patients with symptomatic AF, with no statistical difference in the clinical outcomes. Additional studies using technologies with a better safety profile are needed to evaluate the benefits of CA in elderly patients with AF. (CA (AA . (AF) PV (PV secondgeneration second generation disease 6 1 2 recurrencefree recurrence followup. followup follow up. up QOLF, QOLF , (QOLF) complications 5 p<0.05. p005 p p<0.05 0 05 (p<0.05) 80 10% 10 (10 amiodarone 13 3 1. 60% 60 (60 amiodarone, 0.119 0119 119 71 Q1 Q (Q1 566 Q3 730. 730 730) 834 83 4 83.4 677 67 7 67.7 0.073 0073 073 0.001, 0001 0.001 001 0.001) groups test 25 MRI (AF (QOLF p00 p<0.0 (p<0.05 8 (1 (6 0.11 011 11 (Q 56 73 83. 67. 0.07 007 07 000 0.00 00 p0 p<0. (p<0.0 ( 0.1 01 0.0 p<0 (p<0. 0. p< (p<0 (p<