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Resumo O objetivo deste trabalho foi avaliar se a puberdade e o parto alteram a contagem de folículos antrais (CFA) em fêmeas Nelore (Bos indicus) de corte. Foram realizados dois experimentos para comparar a CFA entre os períodos pré-púbere e púbere e entre os períodos pré-púbere e pós-parto. A CFA e a concentração folicular não diferiram entre os períodos pré-púbere e púbere. Entretanto, a área ovariana foi maior após a puberdade. Além disso, a CFA e a área do ovário foram maiores no período pós-parto quando comparado ao pré-púbere. Portanto, a CFA não é afetada pela puberdade de novilhas Nelore, mas aumenta significativamente quando as novilhas pré-púberes se tornam vacas. (CFA Bos indicus corte prépúbere pré pósparto. pósparto pós parto. Entretanto disso prépúbere. Portanto prépúberes púberes vacas

Abstract The objective of this work was to investigate whether puberty and parturition alter the antral follicle count (AFC) in female Nelore (Bos indicus) beef cattle. Two experiments were carried out to compare AFC between the prepubertal and pubertal periods and between the prepubertal and postpartum periods. AFC and follicle concentrations did not differ between the prepubertal and pubertal periods. However, the ovarian area increased after puberty. In addition, AFC and the ovarian area were greater in the postpartum than in the prepubertal period. Therefore, AFC is not affected by the puberty of Nelore heifers, but increases significantly when the prepubertal heifers become cows. (AFC Bos indicus cattle However addition period Therefore cows

https://doi.org/10.1590/s1678-3921.pab2023.v58.03140

Geometria orofaríngea e parâmetros acústicos vocais de indivíduos hígidos e com doença de Parkinson
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Silva, Joice Maely Souza da

; Gomes, Adriana de Oliveira Camargo

; Coriolano, Maria das Graças Wanderley de Sales

; Teixeira, Julianne Pitanga

; Lima, Hellen Vasconcelos Silva Leal de

; Paulino, Clarissa Evelyn Bandeira

; Silva, Hilton Justino da

; Lira, Zulina Souza de

CoDAS

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2023, Volume 35 N. 2 elocation e20210304

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO Objetivo verificar se existem diferenças nas medidas acústicas e da geometria orofaríngea entre indivíduos hígidos e pessoas com Doença de Parkinson, segundo a idade e sexo e investigar se há correlações entre as medidas geométricas orofaríngeas nessa população. Método participaram 40 indivíduos, sendo 20 com diagnóstico de Doença de Parkinson e 20 indivíduos hígidos, pareados por faixa etária, sexo e índice de massa corporal. As variáveis acústicas estudadas foram frequência fundamental, jitter, shimmer, glottal-to-noise excitation ratio, ruído e média da intensidade. As variáveis geométricas da orofaringe foram aferidas por faringometria acústica. Resultados as variáveis geométricas foram menores no grupo com Doença de Parkinson e os idosos com Doença de Parkinson apresentaram menor área da junção orofaríngea que os idosos hígidos. Com relação aos parâmetros acústicos vocais, o valor da frequência fundamental foi menor no sexo masculino, no grupo com Doença de Parkinson e os valores de jitter foram maiores no grupo não idoso dos sujeitos com Doença de Parkinson. Houve correlação positiva moderada entre o comprimento e volume da cavidade oral, comprimento da cavidade faríngea e o comprimento do trato vocal e do volume da cavidade faríngea e o volume do trato vocal. Conclusão indivíduos com Doença de Parkinson apresentaram menores valores de área glótica e área da junção orofaríngea, comparativamente aos hígidos. Quando distribuídos por faixa etária e sexo, a frequência fundamental foi menor no grupo com doença de Parkinson, na população masculina. Houve correlação positiva moderada entre as medidas de comprimento e volume da orofaringe, na amostra estudada. 4 2 corporal shimmer glottaltonoise glottal to noise ratio intensidade acústica vocais masculino oral masculina estudada

ABSTRACT Purpose to verify whether there are differences in acoustic measures and oropharyngeal geometry between healthy individuals and people with Parkinson's disease, according to age and sex, and to investigate whether there are correlations between oropharyngeal geometry measures in this population. Methods 40 individuals participated, 20 with a diagnosis of Parkinson's disease and 20 healthy individuals, matched by age, sex, and body mass index. Acoustic variables included fundamental frequency, jitter, shimmer, glottal-to-noise excitation ratio, noise, and mean intensity. Oropharyngeal geometry variables were measured with acoustic pharyngometry. Results geometry variables were smaller in the group with Parkinson's disease, and older adults with Parkinson's disease had a smaller oropharyngeal junction area than healthy older adults. Regarding acoustic parameters of voice, fundamental frequency values were lower in males with Parkinson's disease, and jitter values were higher in the non-elderly subjects with Parkinson's disease. There was a moderate positive correlation between oral cavity length and volume, pharyngeal cavity length and vocal tract length, and pharyngeal cavity volume and vocal tract volume. Conclusion individuals with Parkinson's disease had smaller glottal areas and oropharyngeal junction areas than healthy individuals. When distributed into sex and age groups, the fundamental frequency was lower in males with Parkinson's disease. There was a moderate positive correlation between oropharyngeal length and volume measures in the study sample. Parkinsons Parkinson s population 4 participated 2 index shimmer glottaltonoise noise ratio intensity pharyngometry voice nonelderly non elderly groups sample

https://doi.org/10.1590/2317-1782/20232021304pt

Glucose and reflexology for pain relief during arterial puncture in neonates: a protocol neonates
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Costa, Thatiane Monick de Souza

; Fernandes, Thatiane Evelyn de Lima

; Silva, Silmara de Oliveira

; Queiroz, Cintia Galvão

; Rocha, Mayara Araújo

; Duarte, Fernando Hiago da Silva

; Dantas, Rodrigo Assis Neves

; Dantas, Daniele Vieira

Revista Gaúcha de Enfermagem

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2022, Volume 43 N. spe elocation e20220103

Abstract: Es En Pt | Text: Es En Pt | PDF: Es | PDF: En | PDF: Pt

RESUMEN Objetivo Presentar un protocolo de estudio para comparar glucosa y reflexología en el alivio del dolor en cuidados intensivos neonatales durante la punción arterial. Método Protocolo de ensayo clínico aleatorizado, controlado, doble ciego, en 30 recién nacidos en cuidados intensivos neonatales en la maternidad de un hospital escuela, que necesiten extracción de sangre por punción arterial. Serán asignados aleatoriamente a un grupo control (25% de glucosa) o a un grupo de intervención (reflexología podal). El resultado primario serán los escores de dolor neonatal durante y después de la punción arterial. Los resultados secundarios serán el tiempo de llanto y la variación en los signos vitales de los recién nacidos durante y después del procedimiento de punción arterial. Número de registro RBR-639bff. Discusión Los resultados de este ensayo proporcionarán nuevos conocimientos sobre la intervención más adecuada para el alivio del dolor neonatal durante los procedimientos dolorosos. arterial aleatorizado controlado ciego 3 escuela 25% 25 (25 podal. podal . podal) RBR639bff. RBR639bff RBRbff RBR 639bff. 639bff bff RBR-639bff dolorosos 2 (2 (

ABSTRACT Objective To present a study protocol to compare glucose and reflexology in pain relief in neonate intensive care during arterial punctures. Methods A randomized, controlled, double-blind clinical trial protocol will be carried out at a teaching hospital maternity, with30 newborns admitted to neonate intensive care who are to undergo blood collection by arterial puncture. They will be randomly assigned to a control group (25% glucose) or an intervention group (foot reflexology). The primary outcome will be neonate pain scores during and after arterial puncture. Secondary outcomes will be crying time and variation in neonates' vital signs during and after the arterial puncture procedure. Registration number RBR-639bff. Discussion The results of this trial will provide new insights into the most appropriate intervention for the relief of neonate pain during painful procedures. punctures randomized controlled doubleblind double blind maternity with with3 25% 25 (25 foot reflexology. . reflexology) neonates procedure RBR639bff. RBR639bff RBRbff RBR 639bff. 639bff bff RBR-639bff procedures 2 (2 (

RESUMO Objetivo Apresentar um protocolo de estudo para comparar a glicose e reflexoterapia no alívio da dor em terapia intensiva neonatal durante a punção arterial. Método Protocolo de ensaio clínico randomizado, controlado, duplo-cego, será realizado em 30recém-nascidos internados em terapia intensiva neonatal de uma maternidade escola que apresentem indicação de coleta de sangue por punção arterial. Serão distribuídos aleatoriamente em grupo controle (glicose 25%) ou grupo intervenção (reflexoterapia podal). O desfecho primário será escores de dor neonatal durante e após a punção arterial. Os desfechos secundários serão o tempo de choro e variação nos sinais vitais dos neonatos durante e após o procedimento da punção arterial. Número do registro RBR-639bff. Discussão Os resultados deste ensaio fornecerão novos conhecimentos sobre a intervenção mais adequada para o alívio da dor neonatal durante procedimentos dolorosos. arterial randomizado controlado duplocego, duplocego duplo cego, cego duplo-cego 30recémnascidos recémnascidos 30recém nascidos recém 25% 25 podal. podal . podal) RBR639bff. RBR639bff RBRbff RBR 639bff. 639bff bff RBR-639bff dolorosos 2

https://doi.org/10.1590/1983-1447.2022.20220103.en

Effect of inulin, medium-chain triglycerides and whey protein isolate on stability and in vitro digestibility of enteral nutrition formulas
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SOUZA, Mariana Wanessa Santana de

; LOPES, Evelyn de Souza Oliveira

; COSENZA, Gustavo Pereira

; ALVARENGA, Verônica Ortiz

; LABANCA, Renata Adriana

; ARAÚJO, Raquel Linhares Bello de

; LACERDA, Inayara Cristina Alves

Food Science and Technology

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dec 2020, Volume 40 N. 4 Pages 854 - 863

Abstract: En | Text: En | PDF: En

Abstract This study aimed to evaluate the impact on digestibility and physical properties of enteral formulas by the addition of variable ingredients. Eight formulas were designed by a simplex-centroid evaluating different concentrations of inulin, medium-chain triglycerides (MCT) and whey protein isolate (WPI). Overall, the eight formulas developed presented mean values of 17.3% of protein, 62.3% of carbohydrates, 11.5% of lipids, a caloric value of 420 kcal/100 g, thus obtaining nutritionally adequate formulas. The emulsion stability of the suspended formulas was affected by all ingredients and the interactions between MCT – inulin, and MCT – WPI were effective in improving this parameter. Besides that, the use of inulin mostly affected the protein digestibility, according to the degree of hydrolysis and the peptide profile. The desirability function (d-value = 0.769) proposed a formulation containing 0.70% of inulin, 1.56% of MCT, and 1.73% of WPI. This proposed solution may improve enteral formulas because this has optimal emulsion stability and protein digestibility, which are essential characteristics for a product to be used by patients under special clinical conditions.

https://doi.org/10.1590/fst.23619 619 downloads

Sofrimento moral de profissionais de enfermagem em um Centro de Atenção Psicossocial
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Oliveira, Carolina Almeida de

; Oliveira, Diúlia Cristina Pantoja de

; Cardoso, Elessandra Miranda

; Aragão, Evelyn de Souza

; Bittencourt, Marina Nolli

Ciência & Saúde Coletiva

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jan 2020, Volume 25 N. 1 Pages 191 - 198

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Resumo O sofrimento moral (SM) é vivenciado por uma pessoa e decorrente da contradição existente entre suas ações e convicções. O objetivo foi analisar o sofrimento moral vivenciado pelos profissionais de enfermagem que atuam no CAPS, bem como descrever e identificar os sentimentos decorrentes do SM e mecanismos de enfrentamento. Trata-se de uma pesquisa com abordagem qualitativa, na qual foi utilizado o método de Análise de Conteúdo de Bardin. Participaram da pesquisa sete profissionais de ambos os turnos de funcionamento do CAPS. Na análise temática de conteúdo das falas desses profissionais, emergiram quatro categorias: Ambiente, como fonte de sofrimento moral; Escassez de financiamento e improvisos; Sentimentos que emergiram das vivências de Sofrimento Moral; e Mecanismos de Enfrentamento dos profissionais. Neste estudo observou-se que os profissionais do CAPS Gentileza de Macapá vivenciam o SM em seu cotidiano, associado às questões do ambiente estrutural e insuficiência de recursos (humanos e materiais), que resultaram em sentimentos de impotência, desânimo, desmotivação e luta. Esse SM fez com que os profissionais pudessem construir mecanismos de enfrentamento, dentre eles o diálogo e o compartilhamento das angústias com a equipe.

Abstract Moral Distress (MD) is experienced by individuals from the contradiction existing between their actions and convictions. This study aimed to analyze the moral distress experienced by nursing professionals working at the CAPS and to describe and identify the feelings resulting from MD and coping mechanisms. This is a qualitative research that adopted the Bardin Content Analysis method. Seven Nursing professionals of both CAPS shifts participated in the research. Four categories emerged from the thematic analysis of the statements of these professionals, as follows: Environment as a source of moral distress; Shortage of funding and improvisations; Feelings that emerged from the experiences of Moral Distress and Coping Mechanisms of professionals. In this study, we observed that Macapá's Gentileza CAPS professionals experience MD in their daily life, associated with structural environment issues and insufficient (human and material) resources that resulted in feelings of helplessness, discouragement, demotivation, and struggle. This MD allowed these professionals to build coping mechanisms against moral distress, such as dialogue and sharing anguish with the team.

https://doi.org/10.1590/1413-81232020251.29132019 2727 downloads

Sofrimento moral de profissionais de enfermagem em um Centro de Atenção Psicossocial
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Oliveira, Carolina Almeida de

; Oliveira, Diúlia Cristina Pantoja de

; Cardoso, Elessandra Miranda

; Aragão, Evelyn de Souza

; Bittencourt, Marina Nolli

Ciência & Saúde Coletiva

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jan 2020, Volume 25 N. 1 Pages 191 - 198

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

https://doi.org/10.1590/1413-81232020251.29132019

Brazilian Nutritional Consensus in Hematopoietic Stem Cell Transplantation: Graft- versus -host disease
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Pereira, Andréa Z

; Vigorito, Afonso Celso

; Almeida, Alessandro de Moura

; Candolo, Alexandre de Almeida

; Silva, Ana Carolina Leão

; Brandão-Anjos, Ana Elisa de Paula

; Sá, Bianca Laselva de

; Souza, Catarina Lôbo Santos de

; Castro Junior, Cláudio Galvão de

; Oliveira, José Salvador Rodrigues de

; Barban, Juliana Bernardo

; Mancilha, Elaine Maria Borges

; Todaro, Juliana

; Lopes, Lilian Pinheiro

; Macedo, Maria Cristina Martins de Almeida

; Rodrigues, Morgani

; Ribeiro, Paulo Cesar

; Silva, Roberto Luiz da

; Roberto, Telma Sigolo

; Rodrigues, Thays de Cássia Ruiz

; Colturato, Vergilio Antonio Rensi

; Paton, Eduardo José de Alencar

; Barros, George Maurício Navarro

; Almeida, Rosana Ducatti Souza

; Moreira, Maria Claudia Rodrigues

; Flowers, Mary Evelyn

einstein (São Paulo)

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2020, Volume 18 elocation eAE4799

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO O Consenso Brasileiro de Nutrição no Transplante de Células Tronco Hematopoiéticas: doença do enxerto contra o hospedeiro foi aprovado pela Sociedade Brasileira de Transplante de Medula Óssea, com a participação de 26 centros brasileiros de transplante de células-tronco hematopoiéticas. O Consenso descreve as principais condutas nutricionais em casos de doença do enxerto contra o hospedeiro, a principal complicação do transplante de células-tronco hematopoiéticas.

ABSTRACT The Brazilian Consensus on Nutrition in Hematopoietic Stem Cell Transplantation: Graft- versus -host disease was approved by Sociedade Brasileira de Transplante de Medula Óssea , with the participation of 26 Brazilian hematopoietic stem cell transplantation centers. It describes the main nutritional protocols in cases of Graft- versus -host disease, the main complication of hematopoietic stem cell transplantation.

https://doi.org/10.31744/einstein_journal/2020ae4799 1552 downloads

Antifungal and anti-biofilm effect of the calcium channel blocker verapamil on non-albicans Candida species
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SCORZONI, LILIANA

; MENEZES, RAQUEL T. DE

; PEREIRA, THAIS C.

; OLIVEIRA, PRISCILA S.

; RIBEIRO, FELIPE DE CAMARGO

; SANTOS, EVELYN LUZIA DE SOUZA

; FUGISAKI, LUCIANA R.O.

; OLIVEIRA, LUCIANE D. DE

; AMORIM, JOSÉ BENEDITO O.

Anais da Academia Brasileira de Ciências

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2020, Volume 92 N. 4 elocation e20200703

Abstract: En | Text: En | PDF: En

Abstract Candida is a human fungal pathogen that causes a wide range of diseases. Candida albicans is the main etiologic agent in these diseases; however, infections can be caused by non-albicans Candida species. Virulence factors such as biofilm production, which protect the fungus from host immunity and anti-fungal drugs, are important for the infection. Therefore, available antifungal drugs for candidiasis treatment are limited and the investigation of new and effective drugs is needed. Verapamil is a calcium channel blocker with an inhibitory effect on hyphae development, adhesion, and colonization of C. albicans. In this study, we investigated the effect of verapamil on cell viability and its antifungal and anti-biofilm activity in non-albicans Candida species. Verapamil was not toxic to keratinocyte cells; moreover, C. krusei, C. parapsilosis, and C. glabrata were susceptible to verapamil with a minimal inhibitory concentration (MIC) of 1250 μM; in addition, this drug displayed fungistatic effect at the evaluated concentrations. After treatment with verapamil, reduced viability, biomass, and mitochondrial activity were observed in biofilms of the non-albicans Candida species C. krusei, C. glabrata, and C. parapsilosis. These findings highlight the importance of the study of verapamil as an alternative treatment for infections caused by non-albicans Candida species.

https://doi.org/10.1590/0001-3765202020200703 536 downloads

Chloroquine and mefloquine resistance profiles are not related to the circumsporozoite protein (CSP) VK210 subtypes in field isolates of Plasmodium vivax from Manaus, Brazilian Amazon
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Pratt-Riccio, Lilian Rose

; Baptista, Bárbara de Oliveira

; Torres, Vanessa Rodrigues

; Bianco-Junior, Cesare

; Perce-Da-Silva, Daiana de Souza

; Riccio, Evelyn Kety Pratt

; Lima-Junior, Josué da Costa

; Totino, Paulo Renato Rivas

; Cassiano, Gustavo Capatti

; Storti-Melo, Luciane Moreno

; Machado, Ricardo Luiz Dantas

; de Oliveira-Ferreira, Joseli

; Banic, Dalma Maria

; Carvalho, Leonardo José de Moura

; Daniel-Ribeiro, Cláudio Tadeu

Memórias do Instituto Oswaldo Cruz

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2019, Volume 114 elocation e190054

Abstract: En | Text: En | PDF: En

BACKGROUND The central repetitive region (CRR) of the Plasmodium vivax circumsporozoite surface protein (CSP) is composed of a repetitive sequence that is characterised by three variants: VK210, VK247 and P. vivax-like. The most important challenge in the treatment of P. vivax infection is the possibility of differential response based on the parasite genotype. OBJECTIVES To characterise the CSP variants in P. vivax isolates from individuals residing in a malaria-endemic region in Brazil and to profile these variants based on sensitivity to chloroquine and mefloquine. METHODS The CSP variants were determined by sequencing and the sensitivity of the P. vivax isolates to chloroquine and mefloquine was determined by Deli-test. FINDINGS Although five different allele sizes were amplified, the sequencing results showed that all of the isolates belonged to the VK210 variant. However, we observed substantial genetic diversity in the CRR, resulting in the identification of 10 different VK210 subtypes. The frequency of isolates that were resistant to chloroquine and mefloquine was 11.8 and 23.8%, respectively. However, we did not observe any difference in the frequency of the resistant isolates belonging to the VK210 subtypes. MAIN CONCLUSION The VK210 variant is the most frequently observed in the studied region and there is significant genetic variability in the CRR of the P. vivax CSP. Moreover, the antimalarial drug sensitivity profiles of the isolates does not seem to be related to the VK210 subtypes.

https://doi.org/10.1590/0074-02760190054 912 downloads

10.

Selection of starter cultures for the production of sour cassava starch in a pilot-scale fermentation process
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Penido, Fernanda Corrêa Leal

; Piló, Fernanda Barbosa

; Sandes, Sávio Henrique de Cicco

; Nunes, Álvaro Cantini

; Colen, Gecernir

; Oliveira, Evelyn de Souza

; Rosa, Carlos Augusto

; Lacerda, Inayara Cristina Alves

Brazilian Journal of Microbiology

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dec 2018, Volume 49 N. 4 Pages 823 - 831

Abstract: En | Text: En | PDF: En

ABSTRACT Sour cassava starch (Polvilho azedo) is obtained from a spontaneous fermentation conducted by microorganisms from raw materials and fermentation tanks. This product is traditionally used in the baking industry for the manufacture of biscuits and Brazilian cheese breads. However, the end of fermentation is evaluated empirically, and the process occurs without standardization, which results in products of inconsistent quality. Predominant microbiota from a cassava flour manufacturer was isolated in order to select starter cultures for the production of sour cassava starch in a pilot-scale fermentation process. Lactic acid bacteria and yeasts were isolated, enumerated and grouped by Restriction Fragment Length Polymorphism, and PCR fingerprinting, respectively. One isolate of each molecular profile was identified by sequencing of the rRNA gene. LAB were prevalent throughout the entire process. Lactobacillus brevis (21.5%), which produced the highest values of acidity, and Lactobacillus plantarum (13.9%) were among the most frequent species. Pichia scutulata (52.2%) was the prevalent yeast and showed amylolytic activity. The aforementioned species were tested as single and mixed starter cultures in a pilot-scale fermentation process for 28 days. L. plantarum exhibited better performance as a starter culture, which suggests its potential for the production of sour cassava starch.

https://doi.org/10.1016/j.bjm.2018.02.001 1425 downloads

11.

Porous Titanium Associated with CaP Coating: In Vivo and In Vitro Osteogenic Performance
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Vasconcellos, Luana Marotta Reis de

; Nascimento, Rodrigo Dias

; Cairo, Carlos Alberto Alves

; Leite, Daniel de Oliveira

; Santos, Evelyn Luzia de Souza

; Campos, Gabriela Esteves

; Prado, Renata Falchete do

; Jardini, Maria Aparecida Neves

; Vasconcellos, Luis Gustavo Oliveira de

; Carvalho, Yasmin Rodarte

Materials Research

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2018, Volume 21 N. 2 elocation e20170557

Abstract: En | Text: En | PDF: En

This in vitro and in vivo study compared different topographies of Ti samples (dense, porosity of 30% and 40%) with or not CaP coating, prepared by powder metallurgy. Osteogenic cells from newborn rat calvaria were plated onto the samples and cell adhesion (24 hours), alkaline phosphatase activity (7 and 10 days) and mineralization nodules (14 days) were assessed. Sixteen rabbits were used for in vivo study. Each animal received three non-treated and three treated implants in the right or left tibia, respectively. Histometric evaluation of bone-implant contact (BIC) were assessed at 1, 2, 4 and 8 weeks. Metallographic analysis revealed porosities of 30% and 40%, with pore size ranging from 250 to 350 µm. Cell adhesion test and ALP revealed similar cell behavior, independently of topography and CaP coating (P > 0.05%). However, CaP coating combined with porosity of 40% influenced positively the mineralized matrix formation (P < 0.05%). CaP-coated implants showed higher BIC than non-CaP implants and BIC was different between the short (1 and 2 weeks) and long (4 and 8 weeks) healing periods (P < 0.05%). The results suggest that CaP coating combined with 40% porosity implants allowed greater osteogenesis in vitro and increased BIC in vivo.

https://doi.org/10.1590/1980-5373-mr-2017-0557 912 downloads

12.

Supplementation of fungal and/or bacterial phytase in broiler diets formulated with reduced phosphorus level and different calcium contents
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Nardelli, Nicole Batelli de Souza

; Naves, Luciana de Paula

; Oliveira, David Henrique de

; Garcia Junior, Antonio Amandio Pinto

; Lima, Eduardo Machado Costa

; Oliveira, Evelyn Cristina de

; Rodrigues, Paulo Borges

Revista Brasileira de Zootecnia

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2018, Volume 47 elocation e20170297

Abstract: En | Text: En | PDF: En

ABSTRACT The objective of the current study was to evaluate the effects of three calcium:available phosphorus (Ca:aP) ratios and different supplementation with phytases from different origins on performance and ash, Ca, and P contents in the tibia of broilers. A total of 900 male broilers (22 to 35 days old) were fed ten dietary treatments in a completely randomized design. A 3 × 3 + 1 factorial scheme was used, corresponding to three Ca:aP ratios (4.5:1.0, 6.0:1.0, and 7.5:1.0) and three different supplementations with phytases from different origins (isolated or combined supplementation with bacterial and fungal phytase), plus a control diet. Regardless of the dietary Ca:aP ratio, the isolated use of bacterial phytase provided better feed conversion than the fungal phytase but did not differ from combined supplementation with bacterial and fungal phytase. However, regardless of the supplemented phytase, the 7.5:1.0 Ca:aP ratio decreased the feed conversion. Best results for bone P deposition were observed using diets containing the 4.5:1.0 Ca:aP and fungal phytase or the 4.5:1 and 6.0:1 Ca:aP ratios using the bacterial phytase. In general, when the parameters of feed conversion, bone ash, and P content in tibia are considered together, diets containing a 4.5:1.0 or 6.0:1.0 Ca:aP ratio and 1,500 FTU kg−1 bacterial phytase, or a 4.5:1.0 Ca:aP ratio using fungal phytase and only 1.0 g kg−1 available phosphorus provide better results.

https://doi.org/10.1590/rbz4720170297 1169 downloads

13.

Assessment of volatile organic compounds from banana Terra subjected to different alcoholic fermentation processes
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CAPOBIANGO, Michely

; DINIZ, Isabela Maia

; ANDRE, Leiliane Coelho

; OLIVEIRA, Evelyn de Souza

; CARDEAL, Zenilda de Lourdes

Food Science and Technology

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sep 2016, Volume 36 N. 3 Pages 510 - 519

Abstract: En | Text: En | PDF: En

Abstract Banana characteristics could provide yeast growth and support the action of yeast to produce beverages. The effects of enzymatic treatment, centrifugation, commercial yeast and two selected strains of Saccharomyces cerevisiae were investigated using the fermented banana Terra. Volatile organic compounds (VOCs) were determined by solid phase microextraction using gas chromatography mass spectrometry. The results showed that the condition employing enzymatic treatment, centrifugation and wet commercial yeast provided maximum ethanol yield (86%) and effiency (98%). Twenty-two compounds of distinct chemical classes were analysed including alcohols, esters, acids and aldehydes. The concentrations of the VOCs differed depending on the fermentation condition and were more influenced by using different yeasts. The limits of detection and quantification ranged from 0.056 to 2.694 mg L–1 and 0.057 to 2.904 mg L–1 respectively. Among the most prevalent VOCs the higher alcohols ranged from 353 to 1017 mg per 100 mL of anhydrous alcohol. The fermented banana studied showed a composition similar to other fermented fruit pulps.

https://doi.org/10.1590/1678-457X.02915 2553 downloads

14.

Aumento na produção de biomassa de levedura em propagador aerado por processo descontínuo e semicontínuo para produção de cachaça
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Mendes, Tiago Antônio de Oliveira

; Pinto, Lucas Martins

; Mendes, Débora de Sena Oliveira

; Malta, Hélia Lucila

; Oliveira, Evelyn de Souza

Brazilian Journal of Food Technology

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jun 2013, Volume 16 N. 2 Pages 81 - 89

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Tradicionalmente, a propagação de leveduras é feita diretamente dentro das dornas de fermentação nas fábricas de cachaça de alambique. Contudo, estas não dispõem de quaisquer dispositivos que permitam otimizar a propagação, na qual a eficiência da aeração é fator primordial para a predominância do metabolismo respiratório, que permite maximizar a reprodução das células e minimizar a formação de etanol. Neste trabalho, avaliou-se o crescimento de leveduras Saccharomyces cerevisiae em um equipamento dotado de sistema de aeração pelo processo de batelada simples (descontínuo) e pelo processo semicontínuo, utilizando-se um meio complexo ou um meio agroindustrial. O uso do equipamento com aeração permitiu maior conversão de substrato em célula e reduziu o nível de etanol e acidez produzidos. A propagação realizada pelo processo semicontínuo foi mais eficiente do que o de batelada simples. A utilização de um meio agroindustrial suplementado com uma fonte proteica, tal como geralmente é realizado na propagação de leveduras para produção de cachaça de alambique, forneceu maiores aumentos de biomassa e melhores parâmetros de propagação, quando comparado com um meio complexo. Estes resultados contribuirão para o desenvolvimento de um protocolo operacional de propagação de fermento a ser utilizado para produção de cachaça de alambique.

Traditionally, the yeast is propagated directly in the fermentation vats in sugarcane spirit factories. However, they have no devices to optimize the propagation, in which the aeration efficiency is a key factor in the prevalence of the respiratory metabolism to maximize yeast reproduction and minimize the formation of ethanol. This study evaluated the growth of the yeast Saccharomyces cerevisiae in equipment with both simple batch and semi-continuous aeration systems using complex or agro industrial media. The use of aerated equipment allowed for greater substrate conversion to cells and reduced the levels of ethanol and acids produced. The propagation carried out by the semi-continuous process was more efficient than that with the simple batch system. The use of an agro industrial medium supplemented with a protein source, as usually done in the propagation of yeast for the production of sugarcane spirit, provided the greatest increases in biomass and the best parameters for propagation as compared to a complex medium. These results will contribute to the development of an operational protocol for propagation of the yeast to be used in the production of sugarcane spirit.

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15.

Antibodies against the Plasmodium falciparum glutamate-rich protein from naturally exposed individuals living in a Brazilian malaria-endemic area can inhibit in vitro parasite growth
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Pratt-Riccio, Lilian Rose

; Bianco-Junior, Cesare

; Totino, Paulo Renato Rivas

; Perce-Da-Silva, Daiana De Souza

; Silva, Luciene Aquino

; Riccio, Evelyn Kety Pratt

; Ennes-Vidal, Vítor

; Neves-Ferreira, Ana Gisele Costa

; Perales, Jonas

; Rocha, Surza Lucia Gonçalves Da

; Dias-Da-Silva, Fabrício

; Ferreira-da-Cruz, Maria de Fátima

; Daniel-Ribeiro, Cláudio Tadeu

; Oliveira-Ferreira, Joseli De

; Theisen, Michael

; Carvalho, Leonardo José De Moura

; Banic, Dalma Maria

Memórias do Instituto Oswaldo Cruz

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aug 2011, Volume 106 Suppl. 1 Pages 34 - 43

Abstract: En | Text: En | PDF: En

The glutamate-rich protein (GLURP) is an exoantigen expressed in all stages of the Plasmodium falciparum life cycle in humans. Anti-GLURP antibodies can inhibit parasite growth in the presence of monocytes via antibody-dependent cellular inhibition (ADCI), and a major parasite-inhibitory region has been found in the N-terminal R0 region of the protein. Herein, we describe the antiplasmodial activity of anti-GLURP antibodies present in the sera from individuals naturally exposed to malaria in a Brazilian malaria-endemic area. The anti-R0 antibodies showed a potent inhibitory effect on the growth of P. falciparum in vitro, both in the presence (ADCI) and absence (GI) of monocytes. The inhibitory effect on parasite growth was comparable to the effect of IgGs purified from pooled sera from hyperimmune African individuals. Interestingly, in the ADCI test, higher levels of tumour necrosis factor alpha (TNF-α) were observed in the supernatant from cultures with higher parasitemias. Our data suggest that the antibody response induced by GLURP-R0 in naturally exposed individuals may have an important role in controlling parasitemia because these antibodies are able to inhibit the in vitro growth of P. falciparum with or without the cooperation from monocytes. Our results also indicate that TNF-α may not be relevant for the inhibitory effect on P. falciparum in vitro growth.

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