Abstract To date, hydroxyurea is the only effective and safe drug that significantly reduces morbidity and mortality of individuals with Sickle cell disease. Twenty years of real-life experience has demonstrated that hydroxyurea reduces pain attacks, vaso-occlusive events, including acute chest syndrome, the number and duration of hospitalizations and the need for transfusion. The therapeutic success of hydroxyurea is directly linked to access to the drug, the dose used and adherence to treatment which, in part, is correlated to the availability of hydroxyurea. This consensus aims to reduce the number of mandatory exams needed to access the drug, prioritizing the requesting physician's report, without affecting patient safety. date disease reallife real life attacks vasoocclusive vaso occlusive events syndrome transfusion which part physicians physician s report safety

Introduction The hemogram and hemogram-derivative ratios (HDRs) are becoming markers of the severity and mortality of COVID-19. We evaluated the hemograms and serial weekly HDRs [neutrophil-lymphocyte ratio (NLR), monocyte-lymphocyte ratio (MLR), platelet-lymphocyte ratio (PLR), neutrophil-platelet ratio (NPR) and systemic immune-inflammatory index (SII)] in the survivors and non-survivors of COVID-19. Methods We retrospectively reviewed the medical notes and serial hemograms of real-time reverse-transcription polymerase chain reaction (RT-PCR)-confirmed COVID-19 adults hospitalized from April 2020 to March 2021 from the time of diagnosis to the 3rd week of diagnosis. Results Of the 320 adults, 257 (80.3%) were survivors and had a lower mean age than the non-survivors (57.73 vs. 64.65 years, p < 0.001). At diagnosis, the non-survivors had lower hematocrit (p = 0.021), and lymphocyte (p = 0.002) and basophil (p = 0.049) counts and the hematocrit showed a p-value (Is this what you meant???) of 0.021); higher NLR (p < 0.001), PLR (p = 0.047), NPR (p = 0.022) and SII (p = 0.022). Using general linear models, the survivors and non-survivors showed significant variations with weekly lymphocyte count (p < 0.001), neutrophil count (p = 0.005), NLR (p = 0.009), MLR (p = 0.010) and PLR (p = 0.035). All HDRs remained higher in the non-survivors in the 2nd week and 3rd week of diagnosis and the HDRs were higher in the intubated patients than in the non-intubated patients. The NLR and SII were more efficient predictors of mortality in COVID-19 patients. Conclusions This study shows that serial lymphocyte and neutrophil counts, NLR, PLR, MLR, NPR and SII could serve as good and easily accessible markers of severity and predictors of outcomes in COVID-19 patients and should be used for the monitoring of treatment response. hemogramderivative derivative (HDRs COVID19. COVID19 COVID 19. 19 neutrophillymphocyte , (NLR) monocytelymphocyte monocyte (MLR) plateletlymphocyte platelet (PLR) neutrophilplatelet (NPR immuneinflammatory immune inflammatory (SII) nonsurvivors non realtime real reversetranscription reverse transcription RTPCRconfirmed RT PCR confirmed COVID-1 202 rd 32 25 80.3% 803 80 3 (80.3% 57.73 5773 57 73 (57.7 vs 6465 64 65 64.6 years 0.001. 0001 0.001 . 0 001 0.001) 0.021, 0021 0.021 021 0.021) 0.002 0002 002 0.049 0049 049 pvalue value Is meant meant??? 0.001, 0.047, 0047 0.047 047 0.047) 0.022 0022 022 0.022. models 0.005, 0005 0.005 005 0.005) 0.009, 0009 0.009 009 0.009) 0.010 0010 010 0.035. 0035 0.035 035 0.035) nd nonintubated response COVID1 1 (NLR (MLR (PLR (SII COVID- 20 2 80.3 8 (80.3 57.7 577 5 7 (57. 646 6 64. 000 0.00 00 0.02 02 0.04 004 04 meant?? 0.01 01 003 0.03 03 80. (80. 57. (57 0.0 meant? (80 (5 0. (8 (

Abstract We investigated the Biogran on bone repair and metabolism at several time-intervals upon grafting into rat tibia artificial defects. The biomaterial was thoroughly characterized in vitro, and its dissolution behavior upon immersion was assessed in simulated body fluid (SBF) solution for 1, 3, 7, 14, and 30 days. Biogran was also assessed by in vitro hydroxyapatite formation in SBF solution, which is a marker for bioactive behavior. In vivo, distal and proximal bone defects were performed in the Wistar rat's right tibia and filled according to the experimental groups: I) negative control, no filling; II) positive control, 10 mg of autogenous bone; and III) 10 mg of Biogran. Animals were euthanized at 1, 2, 3, 4, 7, and 10 weeks. Bone neoformation was analyzed using histomorphometry (proximal defect), and local levels of bone morphogenetic protein 2 (BMP-2) were measured using the ELISA assay (distal defect). In vitro, the Biogran sample showed a fast dissolution rate within the first 7 days, parallel to the formation of the hydroxyapatite layer. In vivo, the sample was progressively resorbed at a higher rate within the first month until it became almost absent at week 10th. The sample presented similar or higher bone neoformation concerning the autogenous bone. BMP-2 levels were sustained in the Biogran group (around 200 pg/mg) and detected until the last experimental time with a significant difference compared with the controls. Results suggest Biogran is a candidate for hard tissue engineering even in highly active bone remodeling sites. timeintervals intervals (SBF 1 3 14 days vivo rats s groups I control filling II III 4 weeks defect, defect , defect) BMP2 BMP (BMP-2 defect. . layer 10th th BMP- around 20 pg/mg pgmg pg controls sites (BMP- (BMP

RESUMO Objetivo: desenvolver e validar uma tecnologia educativa audiovisual na forma de vídeo para agregar conhecimentos científicos e práticos aos profissionais de Enfermagem durante o preparo e a administração de medicamentos orais via sonda enteral. Método: trata-se de um estudo metodológico, alicerçado no referencial teórico de Fleming, Reynolds e Wallace. Realizado em Ribeirão Preto, São Paulo, Brasil, de setembro de 2022 a agosto de 2023. A população foi composta por sete especialistas, sendo três enfermeiras, duas farmacêuticas e duas nutricionistas. Estudo desenvolvido em três fases: “Pré-produção” - revisão da literatura e elaboração do roteiro; “Produção” - validação do roteiro e gravação do vídeo; e, “Pós-produção” - validação do vídeo. A aparência e o conteúdo do material foram validados pela técnica Delphi e pelo Índice de Validade de Conteúdo maior ou igual a 70% (IVC ≥0,7). Resultados: o roteiro/script/storyboard do vídeo foi desenvolvido e validado por sete especialistas, com 94,2% de concordância. O vídeo foi validado e atingiu 99,2% de concordância entre os especialistas nos quesitos funcionalidade, usabilidade, eficiência, técnica audiovisual, ambiente e procedimentos. Os especialistas sugeriram organizar o vídeo em três partes, as quais envolveram considerações gerais relacionadas às sondas enterais (Vídeo 1), cuidados de Enfermagem relacionados ao preparo (Vídeo 2) e à administração de medicamentos em pacientes adultos e idosos com sonda (Vídeo 3). Conclusão: a tecnologia educativa audiovisual desenvolvida neste estudo na forma de vídeo pode contribuir com a capacitação de profissionais de nível médio e superior, bem como de estudantes dos cursos técnicos e de graduação em Enfermagem. Objetivo enteral Método tratase trata se metodológico Fleming Wallace Preto Paulo Brasil 202 2023 enfermeiras nutricionistas fases Préprodução Pré produção “Pré-produção Produção “Produção Pósprodução Pós “Pós-produção 70 IVC ≥0,7. 07 ≥0,7 . 0 7 ≥0,7) Resultados roteiroscriptstoryboard script storyboard 942 94 2 94,2 992 99 99,2 funcionalidade usabilidade eficiência procedimentos partes Vídeo 1, 1 , 1) 3. 3 3) Conclusão superior 20 ≥0, 9 94, 99, ≥0 ≥

ABSTRACT Objective: to develop and validate an audiovisual educational technology in the form of video to add scientific and practical knowledge to nursing professionals during oral medication preparation and administration via feeding tube. Method: this is a methodological study based on the theoretical framework of Fleming, Reynolds and Wallace, held in Ribeirão Preto, São Paulo, Brazil, from September 2022 to August 2023. The population was made up of seven experts, three nurses, two pharmacists and two nutritionists. The study was developed in three phases: “Pre-production” - literature review and script preparation; “Production” - script validity and video recording; and “Post-production” - video validity. Material appearance and content were validated using the Delphi technique and a Content Validity Index greater than or equal to 70% (CVI ≥0.7). Results: the video script/storyboard was developed and validated by seven experts, with 94.2% agreement. The video was validated and reached 99.2% agreement among experts in terms of functionality, usability, efficiency, audiovisual technique, environment and procedures. Experts suggested organizing the video into three parts, which involved general considerations related to feeding tubes (Video 1), nursing care related to preparation (Video 2) and medication administration in adult and elderly patients with a tube (Video 3). Conclusion: the audiovisual educational technology developed in this study in the form of video can contribute to training high school and higher education professionals as well as students of vocational training and undergraduate courses in nursing. Objective Method Fleming Wallace Preto Paulo Brazil 202 2023 nurses nutritionists phases Preproduction Pre production “Pre-production Production “Production recording Postproduction Post “Post-production 70 CVI ≥0.7. 07 ≥0.7 . 0 7 ≥0.7) Results scriptstoryboard storyboard 942 94 2 94.2 992 99 99.2 functionality usability efficiency procedures parts Video 1, 1 , 1) 3. 3 3) Conclusion 20 ≥0. 9 94. 99. ≥0 ≥

RESUMEN Objetivo: desarrollar y validar una tecnología educativa audiovisual en forma de video para agregar conocimientos científicos y prácticos a los profesionales de enfermería durante la preparación y administración de medicamentos orales vía sonda enteral. Método: se trata de un estudio metodológico, basado en el marco teórico de Fleming, Reynolds y Wallace. Realizado en Ribeirão Preto, São Paulo, Brasil, de septiembre de 2022 a agosto de 2023. La población estuvo compuesta por siete especialistas, tres enfermeros, dos farmacéuticos y dos nutricionistas. Estudio desarrollado en tres fases: “Preproducción” - revisión de literatura y preparación de guión; “Producción” - validación de guión y grabación de vídeo; y “Postproducción” - validación de vídeo. La apariencia y contenido del material fueron validados mediante la técnica Delphi y el Índice de Validez de Contenido mayor o igual al 70% (IVC ≥0,7). Resultados: el guión/script/storyboard del vídeo fue desarrollado y validado por siete expertos, con un 94,2% de acuerdo. El vídeo fue validado y alcanzó un 99,2% de acuerdo entre expertos en cuanto a funcionalidad, usabilidad, eficiencia, técnica audiovisual, entorno y procedimientos. Los expertos sugirieron organizar el video en tres partes, que involucraron consideraciones generales relacionadas con las sondas enterales (Video 1), cuidados de enfermería relacionados con la preparación (Video 2) y administración de medicamentos en pacientes adultos y ancianos portadores de sonda (Video 3). Conclusión: la tecnología educativa audiovisual desarrollada en este estudio en forma de video puede contribuir a la formación de profesionales de la educación secundaria y superior, así como de estudiantes de cursos técnicos y de pregrado en enfermería. Objetivo enteral Método metodológico Fleming Wallace Preto Paulo Brasil 202 2023 especialistas enfermeros nutricionistas fases Preproducción “Preproducción Producción “Producción Postproducción “Postproducción 70 IVC ≥0,7. 07 ≥0,7 . 0 7 ≥0,7) Resultados guiónscriptstoryboard script storyboard 942 94 2 94,2 992 99 99,2 funcionalidad usabilidad eficiencia procedimientos partes Video 1, 1 , 1) 3. 3 3) Conclusión superior 20 ≥0, 9 94, 99, ≥0 ≥

Resumo O objetivo deste trabalho foi determinar os níveis de acidez para obter os atributos químicos do solo adequados à máxima produção de frutos de amoreiras-pretas cultivadas em solos ácidos submetidos a doses crescentes de calcário. Foram avaliadas as cultivares de amoreira-preta BRS Tupy, Brazos, Guarani e BRS Xavante nos solos Latossolo Vermelho Distroférico (LVdf) e Latossolo Vermelho distrófico (LVd), aos quais foram aplicadas quatro doses de calcário dolomítico (0, 1,5, 3,0 e 6,0 Mg ha-1). Foram avaliados o efeito da calagem sobre os atributos químicos do solo, em arranjo fatorial 2×4 (solos e doses de calcário), bem como o teor de nutrientes foliares e a produção de frutos, em arranjo fatorial 2×4×4 (solos, doses de calcário e cultivares). A produção máxima de frutos foi obtida com 3,3 Mg ha-1 de calcário dolomítico. As cultivares de amoreira-preta apresentaram alta demanda por Ca, Mg e saturação por bases e baixa tolerância ao alumínio nos solos. A partir de 3,3 Mg ha-1 de calcário dolomítico, a produção máxima de frutos de amoras-pretas cultivadas em solos ácidos é obtida com pH 5,6, saturação máxima tolerada de alumínio de 6%, concentração de Ca e Mg de 45,3 mmolc kg-1 e saturação por bases de 48,3%. amoreiraspretas amoreiras pretas amoreirapreta amoreira preta Tupy Brazos LVdf (LVdf LVd, LVd , (LVd) 0, 0 (0 15 1 5 1,5 30 3 3, 60 6 6, ha1. ha1 ha . ha-1) 24 2 4 2× calcário, calcário) 244 2×4× solos, cultivares. cultivares) 33 ha- amoraspretas amoras 56 5,6 6% 453 45 45, kg1 kg kg- 483 48 48,3% (LVd ( 1, 5, 48,3 48,

Abstract The objective of this work was to determine the acidity levels to obtain adequate soil chemical attributes for the maximum fruit yield of blackberry trees grown in acidic soils subjected to increasing limestone rates. The BRS Tupy, Brazos, Guarani, and BRS Xavante blackberry cultivars were evaluated on the Typic Hapludox (LVdf) and Rhodic Hapludox (LVd) soils, to which four rates of dolomitic limestone were applied (0, 1.5, 3.0, and 6.0 Mg ha-1). The effect of liming on soil chemical attributes, in a 2×4 factorial arrangement (soils and limestone rates), as well as leaf nutrient content and fruit yield, in a 2×4×4 factorial arrangement (soils, limestone rates, and cultivars), were evaluated. The maximum fruit yield was obtained with 3.3 Mg ha-1 dolomitic limestone. The blackberry cultivars showed a high demand for Ca, Mg, and base saturation and a low tolerance to aluminum in the soils. From 3.3 Mg ha-1 dolomitic limestone, the maximum fruit yield of blackberry trees grown in acidic soils is obtained with pH 5.6, maximum tolerated aluminum saturation of 6%, Ca and Mg concentration of 45.3 mmolc kg-1, and base saturation of 48.3%. Tupy Brazos Guarani LVdf (LVdf LVd (LVd 0, 0 (0 15 1 5 1.5 30 3 3.0 60 6 6. ha1. ha1 ha . ha-1) 24 2 4 2× , rates) 244 2×4× cultivars, cultivars) 33 3. ha- 56 5.6 6% 453 45 45. kg1, kg1 kg 1, kg-1 483 48 48.3% ( 1. 5. kg- 48.3 48.

Abstract Introduction The time elapsed from diagnosis to hematopoietic stem cell transplantation (HSCT) is influenced by numerous factors. In Brazil, patients using the public health system are also dependent on the availability of HSCT-specific beds in the hematology ward. Objective and Methods We conducted a cohort study of listed patients who underwent allogeneic HSCT at a Brazilian public hospital to investigate the impact of the waitlist time on post-HSCT survival. Results The median time from diagnosis to HSCT was 19 months (IQR, 10 - 43), of which 6 months (IQR, 3 - 9) were spent on the waitlist. The time on the waitlist for HSCT appeared to influence mainly the survival of adult patients (≥ 18 years), with an increasing risk according to this time (RR, 3.53 and 95%CI, 1.81 - 6.88 for > 3 and ≤ 6 months; RR 5.86 and 95%CI, 3.26 - 10.53 for > 6 and ≤ 12 months, and; RR 4.24 and 95%CI, 2.32 - 7.75 for > 12 months). Conclusion Patients who remained on the waitlist for less than 3 months had the highest survival (median survival, 856 days; IQR, 131 - 1607). The risk of reduced survival was about 6-fold higher (95%CI, 2.8 - 11.5) in patients with malignancies. (HSCT factors Brazil HSCTspecific specific ward postHSCT post 1 IQR (IQR 43, 43 , 43) 9 ≥ ( years, years years) RR, (RR 353 53 3.5 95CI CI 95 95%CI 181 81 1.8 688 88 6.8 586 5 86 5.8 326 26 3.2 1053 10.5 424 4 24 4.2 232 2 32 2.3 775 7 75 7.7 months. . months) 85 days 13 1607. 1607 1607) 6fold fold (95%CI 28 8 2. 11.5 115 11 malignancies 35 3. 1. 68 6. 58 5. 105 10. 42 4. 23 77 7. 160 11. 16

RESUMO Objetivos: Identificar a frequência de violência recorrente nos casos notificados no Espírito Santo e os fatores associados. Métodos: Estudo transversal. Foram incluídos os dados de violência no Espírito Santo no período de 2011 a 2018, do Sistema de Informação de Agravos de Notificação, e, realizadas análises estatísticas por meio do teste do qui-quadrado e regressão de Poisson. Resultados: 54,2% (N:14.966) dos casos de violências notificadas são de repetição, e, maiores prevalências estiveram associadas ao sexo feminino (RP: 1,54), a vítima criança (RP: 1,29) ou idosa (RP: 1,25), a presença de deficiências/transtornos (RP: 1,42) e a ocorrência em zona urbana/periurbana (RP: 1,10). Além disso, nota-se maior frequência do agravo por agressor único (RP: 1,20), homem (RP:1,23), com 25 anos e mais (RP: 1,09), conhecido da vítima (RP: 2,81) e na residência (RP: 1,69). Conclusão: Os casos notificados de violência apresentaram uma alta frequência de recorrência, e estiveram associados às características estudadas da vítima, do agressor e do evento. Objetivos Métodos transversal periodo peri odo 201 2018 Notificação quiquadrado qui quadrado Poisson Resultados 542 54 2 54,2 N14.966 N14966 N 14.966 14 966 (N:14.966 repetição RP (RP 1,54, 154 1,54 , 1 1,54) 1,29 129 29 1,25, 125 1,25 1,25) deficiênciastranstornos deficiências transtornos 1,42 142 42 urbanaperiurbana urbana periurbana 1,10. 110 1,10 . 10 1,10) disso notase nota se 1,20, 120 1,20 20 1,20) RP1,23, RP123 1,23 23 (RP:1,23) 1,09, 109 1,09 09 1,09) 2,81 281 81 1,69. 169 1,69 69 1,69) Conclusão recorrência evento 5 54, N14 N14.96 N1496 14966 14.96 96 (N:14.96 15 1,5 1,2 12 1,4 4 11 1,1 RP1 RP1,23 RP12 123 (RP:1,23 1,0 0 2,8 28 8 16 1,6 6 N1 N14.9 N149 1496 14.9 9 (N:14.9 1, RP1,2 (RP:1,2 2, N14. 149 14. (N:14. RP1, (RP:1, (N:14 (RP:1 (N:1 (N: (N

ABSTRACT Objectives: Identify the frequency of recurrent violence in cases reported in Espírito Santo and the associated factors. Methods: Cross-sectional study. Which included data on violence in Espírito Santo from 2011 to 2018, from the Notifiable Diseases Information System, and, Statistical analyzes were performed using the chi-square test and Poisson regression. Results: 54.2% (N:14.966) of reported cases of violence are repeated, and higher prevalences were associated with female sex (PR:1.54), child (PR: 1.29) or elderly victims (PR: 1.25), the presence of disabilities/disorders (PR:1.42) and occurrence in urban/peri-urban areas (PR: 1.10). Furthermore, there is a greater frequency of injuries caused by a single aggressor (PR: 1.20), man (PR: 1.28), aged 25 or over (PR: 1.09), known to the victim (PR: 2.81) and at home (PR: 1.69). Conclusion: The reported cases of violence showed a high frequency of recurrence, and were associated with the studied characteristics of the victim, the aggressor and the event. Objectives factors Methods Crosssectional Cross sectional study 201 2018 System chisquare chi square regression Results 542 54 2 54.2 N14.966 N14966 N 14.966 14 966 (N:14.966 repeated PR1.54, PR154 PR 1.54 , 1 (PR:1.54) (PR 1.29 129 29 1.25, 125 1.25 1.25) disabilitiesdisorders disabilities disorders PR1.42 PR142 1.42 42 (PR:1.42 urban/periurban urbanperiurban urban/peri urban peri 1.10. 110 1.10 . 10 1.10) Furthermore 1.20, 120 1.20 20 1.20) 1.28, 128 1.28 28 1.28) 1.09, 109 1.09 09 1.09) 2.81 281 81 1.69. 169 1.69 69 1.69) Conclusion recurrence event 5 54. N14 N14.96 N1496 14966 14.96 96 (N:14.96 PR1 PR1.54 PR15 154 1.5 (PR:1.54 1.2 12 PR1.4 PR14 142 1.4 4 (PR:1.4 periurban urbanperi 11 1.1 1.0 0 2.8 8 16 1.6 6 N1 N14.9 N149 1496 14.9 9 (N:14.9 PR1.5 15 1. (PR:1.5 PR1. (PR:1. 2. N14. 149 14. (N:14. (PR:1 (N:14 (N:1 (N: (N

RESUMEN Objetivos: Identificar la frecuencia de violencia recurrente en los casos reportados en Espírito Santo y los factores asociados. Métodos: Estudio transversal. Incluyó datos sobre violencia en Espírito Santo de 2011 a 2018, del Sistema de Información de Enfermedades De Declaración Obligatoria, y, Los análisis estadísticos se realizaron mediante la prueba de chi-cuadrado y regresión de Poisson. Resultados: 54,2% (N:14.966) de los casos de violencia reportados son repetidos, y las mayores prevalencias se asociaron con el sexo feminino (RP: 1,54), víctimas infantiles (RP: 1,29) o adultas mayores (RP: 1,25), la presencia de discapacidades/trastornos (RP: 1,42) y la ocurrencia en áreas urbanas/periurbanas (RP: 1,10). Además, existe una mayor frecuencia de lesiones provocadas por un solo agressor (RP: 1,20), hombre (RP:1,28), de 25 años o más (RP:1,09), conocido de la víctima (RP:2,81) y en su domicilio (RP: 1,69). Conclusión: Los casos de violencia denunciados mostraron una alta frecuencia de recurrencia, y estuvieron asociados con las características estudiadas de la víctima, el agresor y el hecho. Objetivos Métodos transversal 201 2018 Obligatoria chicuadrado chi cuadrado Poisson Resultados 542 54 2 54,2 N14.966 N14966 N 14.966 14 966 (N:14.966 repetidos RP (RP 1,54, 154 1,54 , 1 1,54) 1,29 129 29 1,25, 125 1,25 1,25) discapacidadestrastornos discapacidades trastornos 1,42 142 42 urbanasperiurbanas urbanas periurbanas 1,10. 110 1,10 . 10 1,10) Además 1,20, 120 1,20 20 1,20) RP1,28, RP128 1,28 28 (RP:1,28) RP1,09, RP109 1,09 09 (RP:1,09) RP2,81 RP281 2,81 81 (RP:2,81 1,69. 169 1,69 69 1,69) Conclusión recurrencia hecho 5 54, N14 N14.96 N1496 14966 14.96 96 (N:14.96 15 1,5 1,2 12 1,4 4 11 1,1 RP1 RP1,28 RP12 128 (RP:1,28 RP1,09 RP10 109 1,0 0 (RP:1,09 RP2 RP2,8 RP28 281 2,8 8 (RP:2,8 16 1,6 6 N1 N14.9 N149 1496 14.9 9 (N:14.9 1, RP1,2 (RP:1,2 RP1,0 (RP:1,0 RP2, 2, (RP:2, N14. 149 14. (N:14. RP1, (RP:1, (RP:2 (N:14 (RP:1 (N:1 (N: (N

BACKGROUND Corynebacterium auriscanis is a bacterial species frequently isolated from dogs with external otitis or dermatitis and a zoonotic pathogen transmitted by dog bite. It is considered an opportunistic pathogen, but its pathogenicity mechanisms are poorly studied. Comparative genomics can identify virulence and niche factors that could contribute to understanding its lifestyle. OBJECTIVES The objectives of this project was to compare genomes of C. auriscanis to identify genes related to its virulence and lifestyle. METHODS The genome of strain 32 was sequenced using Illumina HiSeq 2500 (Illumina, CA, USA) and assembled using Unicycler. The two other non-redundant genomes from the same species available in GenBank were included in the analysis. All genomes were annotated and checked for taxonomy, assembly quality, mobile elements, CRISPR-Cas systems, and virulence and antimicrobial resistance genes. The virulence genes in the three genomes were compared to the ones from other pathogens commonly isolated with C. auriscanis. FINDINGS The species has 42 virulence factors that can be classified as niche factors, due to the absence of true virulence factors found in primary pathogens. The gene rbpA could confer basal levels of resistance to rifampin. MAIN CONCLUSIONS The absence of true virulence factors in the three genomes suggests C. auriscanis has an opportunistic pathogen lifestyle. bite studied lifestyle C 3 250 Illumina, (Illumina CA USA Unicycler nonredundant non redundant analysis taxonomy quality elements CRISPRCas CRISPR Cas systems 4 rifampin 25 2

ABSTRACT Background: Children presenting enuresis are more likely to be asthmatics. The association between enuresis and sleep-disordered breathing has already been demonstrated and several studies have shown at least partial improvement of two thirds or more of the cases of enuresis adenoidectomy. Studies have already described associations between enuresis and allergies but do not assess the repercussions of allergy treatment in enuretics. Objective: This study aims to evaluated whether asthma treatment alters the course of enuresis and whether there is any predictive factor associated with this improvement. Materials and Methods: Twenty patients (5 - 12 years old) with uncontrolled enuresis and asthma, received treatment for asthma. Children were also assessed for the presence of rhinitis and other allergies. The control of asthma was confirmed by a validated questionnaire and primary enuresis by clinical history and wet night diaries. Patients received only asthma treatment. Results: At least partial improvement of enuresis was observed in 55% of the patients with an increase in 64.4% in the number of dry nights at the end of the study (p=0.01). The “presence of other allergies” and “obstruction seen in nasal endoscopy” positively influenced the improvement of urinary symptoms (OR = 3.350; CI 0.844–13.306) and (OR=1.272; CI 0.480-3.370), respectively. Discussion: Until now, only patients presenting upper airway obstruction were known to benefit from the improvement of urinary symptoms when undergoing treatment for their respiratory problems. In our study, we found at least partial improvement in enuresis in 55% of our patients, with only clinical asthma treatment. Conclusion: Controlling asthma in children with primary enuresis resulted in a significant increase in dry nights. Background asthmatics sleepdisordered sleep disordered adenoidectomy enuretics Objective Methods 5 ( 1 old diaries Results 55 644 64 4 64.4 p=0.01. p001 p p=0.01 . 0 01 (p=0.01) endoscopy OR 3.350 3350 3 350 0.844–13.306 084413306 844 13 306 OR=1.272 OR1272 272 (OR=1.272 0.4803.370, 04803370 0.480 3.370 , 480 370 0.480-3.370) respectively Discussion now problems Conclusion 6 64. p00 p=0.0 (p=0.01 3.35 335 35 0.844–13.30 08441330 84 30 OR=1.27 OR127 27 (OR=1.27 4803 0.4803.370 0480337 0480 0.48 3370 3.37 48 37 0.480-3.370 p0 p=0. (p=0.0 3.3 33 0.844–13.3 0844133 8 OR=1.2 OR12 2 (OR=1.2 0.4803.37 048033 048 0.4 337 0.480-3.37 p=0 (p=0. 3. 0.844–13. 084413 OR=1. OR1 (OR=1. 0.4803.3 04803 04 0. 0.480-3.3 p= (p=0 0.844–13 08441 OR=1 (OR=1 0.4803. 0.480-3. (p= 0.844–1 0844 OR= (OR= 0.4803 0.480-3 (p 0.844– 084 0.480- 0.844 08 0.84 0.8

Resumo O objetivo do estudo foi identificar a percepção dos gestores municipais sobre os recursos extraordinários e seus usos no enfrentamento à COVID-19. Trata-se de estudo de casos múltiplos, de métodos mistos incorporados. Foram realizadas entrevistas semiestruturadas com seis municípios-caso do estado de São Paulo. Foram analisados dados secundários extraídos de sistemas de informação no período de 2020 a 2022. As distintas realidades municipais em termos populacionais, de gastos em saúde e de acesso aos recursos federais permitiram observar diferentes estratégias de gestão financeira e alocação dos recursos. Além dos recursos extraordinários, foi possível identificar, no período analisado, a presença relevante de repasses por Emendas Parlamentares. No contexto de desfinanciamento do SUS, esses recursos e repasses significaram, em muitos casos, a possibilidade de os gestores organizarem o sistema de saúde municipal segundo seu entendimento das necessidades de saúde e as respostas possíveis a essas necessidades. Evidenciou-se que os recursos para o enfrentamento à COVID-19 foram alocados principalmente na média e alta complexidade e na contratação de empresas privadas. COVID19. COVID19 COVID 19. 19 Tratase Trata se múltiplos incorporados municípioscaso municípios caso Paulo 202 2022 populacionais analisado Parlamentares SUS significaram Evidenciouse Evidenciou COVID-1 privadas COVID1 1 20 COVID- 2

Abstract The study examined municipal managers’ perceptions of extraordinary funding and its use to address COVID-19. In this multiple-case, quantitative and qualitative study, using embedded mixed methods, semi-structured interviews were conducted in six case-municipalities in São Paulo state. Secondary data for 2020 to 2022, drawn from information systems, were analysed. The municipalities differed by population, health expenditures and access to federal funding, making it possible to observe different financial management strategies and resource allocation. In addition to the extraordinary funding, considerable budget transfers were found to have been made by Parliamentary Amendments during the study period. In a context where the national Unified Health System is underfunded, extraordinary funding and budget transfers by Parliamentary Amendments often enabled managers to organise municipal health systems to meet their understanding of health needs and possible responses to those needs. Funding to address COVID-19 was allocated mainly to medium- and high-complexity services and to engaging private companies. COVID19. COVID19 COVID 19. 19 multiplecase, multiplecase multiple case, case multiple-case methods semistructured semi structured casemunicipalities state 202 2022 analysed population allocation period underfunded COVID-1 medium highcomplexity high complexity companies COVID1 1 20 COVID- 2

ABSTRACT Background: The rate of tuberculosis (TB) infection among the prison population (PP) in Brazil is 28 times higher than that in the general population, and prison environment favors the spread of TB. Objective: To describe TB transmission dynamics and drug resistance profiles in PP using whole-genome sequencing (WGS). Methods: This was a retrospective study of Mycobacterium tuberculosis cultivated from people incarcerated in 55 prisons between 2016 and 2019; only one isolate per prisoner was included. Information about movement from one prison to another was tracked. Clinical information was collected, and WGS was performed on isolates obtained at the time of TB diagnosis. Results: Among 134 prisoners included in the study, we detected 16 clusters with a total of 58 (43%) cases of M. tuberculosis. Clusters ranged from two to seven isolates with five or fewer single nucleotide polymorphism (SNP) differences, suggesting a recent transmission. Six (4.4%) isolates were resistant to at least one anti-TB drug. Two of these clustered together and showed resistance to rifampicin, isoniazid, and fluoroquinolones, with 100% concordance between WGS and phenotypic drug-susceptibility testing. Prisoners with clustered isolates had a high amount of movement between prisons (two to eight moves) during the study period. Conclusions: WGS demonstrated the recent transmission of TB within prisons in Brazil. The high movement among prisoners seems to be related to the transmission of the same M. tuberculosis strain within the prison system. Screening for TB before and after the movement of prisoners using rapid molecular tests could play a role in reducing transmission. Background (TB (PP 2 Objective wholegenome whole genome WGS. . (WGS) Methods 5 201 2019 tracked collected diagnosis Results 13 1 43% 43 (43% M SNP (SNP differences 4.4% 44 4 (4.4% antiTB anti rifampicin isoniazid fluoroquinolones 100 drugsusceptibility susceptibility testing moves period Conclusions system (WGS 20 (43 4.4 (4.4 10 (4 4. (4. (

Resumen Objetivo Analizar las notificaciones de incidentes ocurridos durante la pandemia de COVID-19. Métodos Estudio con diseño transversal de enfoque cuantitativo del tipo descriptivo exploratorio. Se analizaron 1.466 notificaciones de la gestión de riesgo de un hospital privado, en el período de septiembre de 2020 a septiembre de 2021. Se utilizó el análisis estadístico descriptivo, aplicando la prueba χ2 de Pearson o la prueba de razón de verosimilitud. El margen de error utilizado fue del 5 %. Resultados Se identificaron como incidentes prevalentes la falla en la comunicación (358 - 24,5 %), falla en el uso de sondas y de catéteres (232 – 15,9 %) y falla en el uso de artículos y equipos (132 – 9,1 %). Las circunstancias que pueden ser notificadas totalizaron (55,9 %) de los relatos y, entre ellas, (33,4 %) era una falla en la comunicación. Los eventos adversos totalizaron 416 (28,6 %) y la disminución estuvo relacionada con el daño leve (43,9 %); infección relacionada con la atención a la salud al daño moderado (31 %) y a la falla al usar medicamentos (50 %) como daño grave y defunción. Conclusión La falla en la comunicación fue la circunstancia de riesgo más notificada, seguida de falla al usar medicamentos como evento adverso con daño grave. La unidad de enfermería evidenció la posibilidad de un número más elevado de eventos adversos; mientras que en las unidades de terapia intensiva el grado de daño de los eventos adversos fue superior. COVID19. COVID19 COVID 19. 19 COVID-19 exploratorio 1466 1 466 1.46 privado 202 2021 χ verosimilitud % 358 (35 245 24 24, %, , 232 (23 159 15 9 15, 132 (13 91 9, . 55,9 559 55 (55, ellas 33,4 334 33 4 (33, 41 28,6 286 28 6 (28, 43,9 439 43 (43, 31 (3 50 (5 defunción notificada superior COVID1 COVID-1 146 46 1.4 20 35 2 23 (2 13 (1 55, (55 33, 3 (33 28, (28 43, (43 ( COVID- 14 1. (4

Resumo Objetivo Analisar as notificações de incidentes ocorridos durante a pandemia de COVID-19. Métodos Estudo com delineamento transversal de abordagem quantitativa do tipo descritivo exploratório. Foram analisadas 1.466 notificações à gerência de risco de um hospital privado, no período de setembro de 2020 a setembro de 2021. Utilizou-se a análise estatística descritiva, aplicando o teste Qui-quadrado de Pearson ou o teste da Razão de Verossimilhança. A margem de erro utilizada foi de 5%. Resultados Identificou-se como incidentes prevalentes a falha na comunicação (358 - 24,5%), falha no uso de sondas e cateteres (232 – 15,9%) e falha no uso de artigos e equipamentos (132 – 9,1%). A circunstância notificável totalizou (55,9%) dos relatos e destas, (33,4%) eram falha na comunicação. Os eventos adversos foram em número de 416 (28,6%) e a queda esteve relacionada a dano leve (43,9%); Infecção relacionada à assistência à saúde ao dano moderado (31%) e a falha no uso de medicamentos a (50%) como dano grave e óbito. Conclusão Falha na comunicação foi a circunstância de risco mais notificada, seguida de falha no uso de medicamentos como evento adverso com dano grave. A unidade de enfermaria evidenciou a possibilidade de maior número de eventos adversos; enquanto que nas unidades de terapia intensiva o grau de dano dos eventos adversos foi superior. COVID19. COVID19 COVID 19. 19 COVID-19 exploratório 1466 1 466 1.46 privado 202 2021 Utilizouse Utilizou se descritiva Quiquadrado Qui quadrado Verossimilhança 5 5% Identificouse Identificou 358 (35 24,5%, 245 24,5% , 24 24,5%) 232 (23 15,9% 159 15 9 132 (13 9,1%. 91 9,1% . 9,1%) 55,9% 559 55 (55,9% destas 33,4% 334 33 4 (33,4% 41 28,6% 286 28 6 (28,6% 43,9% 439 43 (43,9%) 31% 31 (31% 50% 50 (50% óbito notificada superior COVID1 COVID-1 146 46 1.4 20 35 (3 24,5 2 23 (2 15,9 13 (1 9,1 55,9 (55,9 33,4 3 (33,4 28,6 (28,6 43,9 (43,9% (31 (50 COVID- 14 1. ( 24, 15, 9, 55, (55, 33, (33, 28, (28, 43, (43,9 (5 (55 (33 (28 (43, (43 (4

Abstract Objective To review notification of incidents that occurred during the COVID-19 pandemic. Methods This is a cross-sectional, exploratory descriptive quantitative study. A total of 1,466 notifications to risk management of a private hospital were analyzed from September 2020 to September 2021. Descriptive statistical analysis was used, applying Pearson’s chi-square test or the likelihood ratio test. The margin of error used was 5%. Results Communication failure (358 - 24.5%), probe and catheter use failure (232 - 15.9%) and article and equipment use failure (132 - 9.1%) were identified as prevalent incidents. The notifiable circumstance totaled 55.9% of reports, and, of these, 33.4% were communication failure. Adverse events were 416 (28.6%), and fall was related to mild damage (43.9%), health care-associated infections, to moderate harm (31%), and medication use failure (50%), to severe harm and death. Conclusion Communication failure was the most reported risk circumstance, followed by medication use failure as an adverse event with severe harm. The nursing unit showed the possibility of a greater number of adverse events, while in Intensive Care Units, the degree of harm from adverse events was higher. COVID19 COVID 19 COVID-1 pandemic crosssectional, crosssectional cross sectional, sectional cross-sectional study 1466 1 466 1,46 202 2021 Pearsons Pearson s chisquare chi square 5 5% 358 (35 24.5%, 245 24.5% , 24 24.5%) 232 (23 15.9% 159 15 9 132 (13 9.1% 91 559 55 55.9 reports these 334 33 4 33.4 41 28.6%, 286 28.6% 28 6 (28.6%) 43.9%, 439 43.9% 43 (43.9%) careassociated care associated infections 31%, 31 31% (31%) 50%, 50 50% (50%) death Units higher COVID1 COVID- 146 46 1,4 20 35 (3 24.5 2 23 (2 15.9 13 (1 9.1 55. 3 33. 28.6 (28.6% 43.9 (43.9% (31% (50% 14 1, ( 24. 15. 9. 28. (28.6 43. (43.9 (31 (50 (28. (43. (5 (28 (43 (4

Resumen Objetivo Identificar la frecuencia de recurrencia de la violencia contra mujeres en los diferentes ciclos de la vida y verificar los factores asociados. Métodos Estudio transversal realizado con datos de notificación de violencia contra el sexo femenino del estado de Espírito Santo, en el período de 2011 a 2018. Se utilizaron datos del Sistema de Información de Agravios de Notificación, por medio de la Ficha de Notificación/Investigación de Violencia Interpersonal y Autoprovocada. Se realizaron análisis estadísticos mediante la regresión de Poisson y la prueba χ2 de Pearson. Resultados La frecuencia de recurrencia de violencia en personas de sexo femenino fue de 58,9 % (IC95 % 58,2-59,5). La violencia de repetición fue 1,26 veces más prevalente en mujeres mayores y 32 % más frecuente entre aquellas con discapacidad. Hubo un 8 % más de notificaciones en área urbana. Respecto al agresor, el sexo masculino prevaleció (razón de prevalencia de 1,37; IC95 % 1,28-1,46), y el grupo de edad más frecuente fue más de 25 años (razón de prevalencia de 1,07; IC95 % 1,03-1,11). La violencia recurrente fue 3,28 veces más cometida por conocidos y por un único agresor (razón de prevalencia de 1,24). Las notificaciones de violencia recurrente fueron un 55 % más prevalentes en la residencia. Conclusión La alta frecuencia de violencia de repetición y las asociaciones con las características estudiadas reflejan la necesidad de atención a este público, así como la importancia de acciones que busquen la detección temprana de la violencia y la atención adecuada a las víctimas y a los agresores familiares, a fin de evitar la perpetuación de las agresiones en la vida cotidiana de las mujeres. asociados Santo 201 2018 Notificación NotificaciónInvestigación Investigación Autoprovocada χ Pearson 589 58 9 58, IC (IC9 58,259,5. 582595 58,2 59,5 . 2 59 5 58,2-59,5) 126 1 26 1,2 3 discapacidad urbana razón 1,37 137 37 IC9 1,281,46, 128146 1,28 1,46 , 28 46 1,28-1,46) 1,07 107 07 1,031,11. 103111 1,03 1,11 03 11 1,03-1,11) 328 3,2 1,24. 124 1,24 24 1,24) residencia público familiares 20 (IC 259 58,259,5 58259 582 595 59, 58,2-59,5 12 1, 1,3 13 281 1,281,46 12814 128 146 1,4 4 1,28-1,46 1,0 10 0 031 1,031,11 10311 103 111 1,1 1,03-1,11 3, 58,259, 5825 58,2-59, 1,281,4 1281 14 1,28-1,4 1,031,1 1031 1,03-1,1 58,259 58,2-59 1,281, 1,28-1, 1,031, 1,03-1, 58,25 58,2-5 1,281 1,28-1 1,031 1,03-1 58,2- 1,28- 1,03-

Resumo Objetivo Identificar a frequência de recorrência da violência contra mulheres nos diferentes ciclos de vida e verificar os fatores associados. Métodos Estudo transversal realizado com dados de notificação de violência contra o sexo feminino do estado do Espírito Santo no período de 2011 a 2018. Utilizaram-se dados do Sistema de Informação de Agravos de Notificação, por meio da Ficha de Notificação/Investigação de Violência Interpessoal e Autoprovocada. Foram realizadas análises estatísticas por meio da regressão de Poisson e do teste do qui-quadrado. Resultados A frequência de recorrência de violência no sexo feminino foi de 58,9% (IC95% 58,2-59,5). A violência de repetição foi 1,26 vez mais prevalente nas idosas e 32% mais frequente entre aquelas com deficiência; as notificações em área urbana foram 8% maiores. Quanto ao agressor, o sexo masculino prevaleceu (razão de prevalência de 1,37; IC95% 1,28-1,46), e faixa etária mais frequente foi mais de 25 anos (razão de prevalência de 1,07; IC95% 1,03-1,11). A violência recorrente foi 3,28 vezes mais cometida por conhecidos e por um agressor único (razão de prevalência de 1,24). As notificações de violência recorrente foram 55% mais prevalentes na residência. Conclusão A alta frequência da violência de repetição e as associações com as características estudadas refletem a necessidade de atenção a esse público, bem como a importância de ações que visem à detecção precoce da violência e à adequada assistência às vítimas e aos agressores familiares, a fim de evitar a perpetuação das agressões no cotidiano das mulheres. associados 201 2018 Utilizaramse Utilizaram se Notificação NotificaçãoInvestigação Investigação Autoprovocada quiquadrado. quiquadrado qui quadrado. quadrado qui-quadrado 589 58 9 58,9 IC95 IC (IC95 58,259,5. 582595 58,2 59,5 . 2 59 5 58,2-59,5) 126 1 26 1,2 32 deficiência 8 maiores razão 1,37 137 37 1,281,46, 128146 1,28 1,46 , 28 46 1,28-1,46) 1,07 107 07 1,031,11. 103111 1,03 1,11 03 11 1,03-1,11) 328 3 3,2 1,24. 124 1,24 24 1,24) 55 residência público familiares 20 58, IC9 (IC9 259 58,259,5 58259 582 595 59, 58,2-59,5 12 1, 1,3 13 281 1,281,46 12814 128 146 1,4 4 1,28-1,46 1,0 10 0 031 1,031,11 10311 103 111 1,1 1,03-1,11 3, (IC 58,259, 5825 58,2-59, 1,281,4 1281 14 1,28-1,4 1,031,1 1031 1,03-1,1 58,259 58,2-59 1,281, 1,28-1, 1,031, 1,03-1, 58,25 58,2-5 1,281 1,28-1 1,031 1,03-1 58,2- 1,28- 1,03-

Abstract Objective To identify the frequency of recurrence of violence against women in different life cycles and to verify associated factors. Methods This is a cross-sectional study carried out with data on report of violence against women in the state of Espírito Santo from 2011 to 2018. Data from the Notifiable Diseases Information System were used, through the Interpersonal and Self-Inflicted Violence Reporting/Investigation Form. Statistical analyzes were performed using Poisson regression and the chi-square test. Results The frequency of recurrence of violence in females was 58.9% (95%CI 58.2-59.5). Repeated violence was 1.26 times more prevalent among elderly women and 32% more frequent among those with disabilities, and reports in urban areas were 8% higher. As for the aggressor, males prevailed (Prevalence Ratio of 1.37; 95%CI 1.28-1.46), and the most frequent age group was over 25 years (Prevalence Ratio of 1.07; 95%CI 1 03-1.11). Recurrent violence was 3.28 times more committed by acquaintances and by a single perpetrator (Prevalence Ratio of 1.24). Reports of recurrent violence were 55% more prevalent in the household. Conclusion The high frequency of recurrent violence and the associations with the characteristics studied reflect the need for attention to this public as well as the importance of actions aimed at the early detection of violence and adequate assistance to victims and family aggressors, in order to avoid the perpetuation of aggression in women’s daily lives. factors crosssectional cross sectional 201 2018 used SelfInflicted Self Inflicted ReportingInvestigation Reporting Investigation Form chisquare chi square test 589 58 9 58.9 95CI CI 95 58.259.5. 582595 58.2 59.5 . 2 59 5 58.2-59.5) 126 26 1.2 32 disabilities 8 higher aggressor Prevalence 1.37 137 37 1.281.46, 128146 1.28 1.46 , 28 46 1.28-1.46) 1.07 107 07 031.11. 03111 03 1.11 11 03-1.11) 328 3 3.2 1.24. 124 1.24 24 1.24) 55 household aggressors womens s lives 20 58. 259 58.259.5 58259 582 595 59. 58.2-59.5 12 1. 1.3 13 281 1.281.46 12814 128 146 1.4 4 1.28-1.46 1.0 10 0 031 031.11 0311 111 1.1 03-1.11 3. 58.259. 5825 58.2-59. 1.281.4 1281 14 1.28-1.4 031.1 03-1.1 58.259 58.2-59 1.281. 1.28-1. 031. 03-1. 58.25 58.2-5 1.281 1.28-1 03-1 58.2- 1.28- 03-

ABSTRACT The use of natural lignocellulosic fiber (NLF) biocomposites for the construction industry has been growing over the years, due to technical and environmental advantages. However, fiber-matrix incompatibility remains a major challenge. Various surface treatments have been investigated to improve fiber-matrix bonding, including sodium lignosulfonate (SLS), a potentially effective and environmentally friendly chemical. In this study, SLS treatment protocols were applied to piassava fibers to evaluate their influence on the fibers. Thermogravimetric Analysis (TG/DTG), X-ray Diffraction (XRD), Scanning Electron Microscopy (SEM/EDS), Fourier-transform Infrared Spectroscopy (FTIR), moisture absorption measurements and tensile tests were performed to characterize the modifications. Results demonstrated, for the first time, the efficiency of SLS to remove extractives from the piassava surface. In general, partial degradation of the cellulosic structure was observed, noticeable by the slight drop in crystallinity index (from 42.80 to 39.82%), and an increase in the TG residual mass (from 21.35 to 31.90%), along with changes in DTG curves. However, a particular SLS treatment using ultrasonic bath was able to fully clean the surface preserving the cellulosic structure, and increasing the strength of fibers (from 386 ± 140 MPa to 524 ± 126 MPa). NLF (NLF years advantages However fibermatrix matrix challenge bonding SLS, , (SLS) chemical study TG/DTG, TGDTG TG/DTG (TG/DTG) Xray X ray XRD, XRD (XRD) SEM/EDS, SEMEDS SEM/EDS SEM EDS (SEM/EDS) Fouriertransform Fourier transform FTIR, FTIR (FTIR) modifications demonstrated time general observed 4280 42 80 42.8 39.82%, 3982 39.82% 39 82 39.82%) 2135 21 35 21.3 31.90%, 3190 31.90% 31 90 31.90%) curves 38 14 52 12 MPa. . MPa) (SLS (TG/DTG (XRD (SEM/EDS (FTIR 428 4 8 42. 398 39.82 3 213 2 21. 319 31.90 9 1 5 39.8 31.9 39. 31.

RESUMEN Objetivo: construir y validar un escenario simulado y un checklist para la evaluación e identificación de enfermería de arritmias desfibrilables en adultos hospitalizados en una Unidad de Cuidados Intensivos. Método: estudio metodológico para la construcción de un escenario simulado y lista de verificación, validado por especialistas, con la aplicación de una prueba piloto en 36 estudiantes de enfermería entre abril y septiembre de 2021 en una universidad pública de Minas Gerais. La etapa de construcción se llevó a cabo mediante el levantamiento de evidencias en la literatura. La etapa de validación ocurrió a partir de la evaluación de los instrumentos por 13 especialistas, de todo el territorio brasileño, siendo interpretados por el cálculo del Coeficiente de Validez de Contenido por punto de corte igual a 0,8 o 80,0%. Para evaluar la consistencia interna se calculó el alfa de Cronbach. Posteriormente, se realizó una prueba piloto y la aplicación de la Escala de Satisfacción y Autoconfianza del Estudiante en el Aprendizaje. Resultados: luego de construir el escenario simulado, se realizaron tres rondas para evaluar el guión (21 ítems) y dos para la lista de verificación (seis ítems), alcanzándose un Coeficiente de Validez de Contenido global de 0,98 y 0,95 respectivamente. Se obtuvo un alfa de Cronbach de 0,79 al final de la evaluación. En la prueba piloto, el escenario se consideró adecuado, con puntuaciones altas de satisfacción y confianza en el aprendizaje, lo que confirma su usabilidad. Conclusión: el instrumento desarrollado por enfermeros para evaluar arritmias desfibrilables en pacientes adultos internados en una Unidad de Cuidados Intensivos es válido en cuanto al contenido y tiene buena consistencia interna. Objetivo Método especialistas 3 202 Gerais literatura 1 brasileño 08 0 8 0, 800 80 80,0% Posteriormente Aprendizaje Resultados 21 (2 ítems seis ítems, , 098 98 0,9 095 95 respectivamente 079 79 0,7 adecuado aprendizaje usabilidad Conclusión 20 80,0 2 ( 09 9 07 7 80,

ABSTRACT Objective: to construct and validate a simulated scenario and checklist for the assessment and identification of shockable arrhythmias by nurses in adults hospitalized in an Intensive Care Unit. Method: this is a methodological study for the construction of a simulated scenario and checklist, validated by experts, with the application of a pilot test on 36 nursing students between April and September 2021, at a public university in Minas Gerais. The construction stage took place by surveying evidence in the literature. The validity stage was based on instrument assessment by 13 experts, from all over Brazil, being interpreted by calculating the Content Validity Coefficient by a cut-off point equal to 0.8 or 80.0%. To assess internal consistency, Cronbach’s alpha was calculated. Subsequently, a pilot test and the Student Satisfaction and Self-Confidence in Learning Scale were applied. Results: after building the simulated scenario, three rounds were performed to assess the script (21 items) and two for the checklist (six items), reaching an overall Content Validity Coefficient of 0.98 and 0.95, respectively. A Cronbach’s alpha of 0.79 was obtained at the end of assessment. In the pilot test, the scenario was considered adequate, with high scores of satisfaction and self-confidence in learning, confirming its usability. Conclusion: the instrument developed by nurses to assess shockable arrhythmias in adult patients admitted to an Intensive Care Unit is valid in terms of content and has good internal consistency. Objective Method experts 3 2021 Gerais literature 1 Brazil cutoff cut off 08 0 8 0. 800 80 80.0% consistency Cronbachs Cronbach s calculated Subsequently SelfConfidence Self Confidence applied Results 21 (2 items six items, , 098 98 0.9 095 95 0.95 respectively 079 79 0.7 adequate selfconfidence self confidence learning usability Conclusion 202 80.0 2 ( 09 9 07 7 20 80.

RESUMO Objetivo: construir e validar um cenário simulado e checklist para avaliação e identificação de arritmias chocáveis pela enfermagem em adultos internados em unidade de terapia intensiva. Método: estudo metodológico para construção de cenário simulado e checklist, validado por especialistas, com aplicação de teste piloto em 36 estudantes de enfermagem entre abril e setembro de 2021, em uma Universidade Pública de Minas Gerais. A etapa de construção ocorreu por levantamento de evidências na literatura. A etapa de validação se deu a partir da avaliação dos instrumentos por 13 especialistas, provenientes de todo o território brasileiro, sendo interpretados pelo cálculo do Coeficiente de Validade de Conteúdo por ponto de corte igual a 0,8 ou 80,0%. Para avaliar a consistência interna, foi calculado o alpha de Cronbach. Posteriormente, foram realizados o teste piloto e a aplicação da Escala de Satisfação dos estudantes e autoconfiança na aprendizagem. Resultados: após a construção do cenário simulado, foram realizadas três rodadas para avaliação do roteiro (21 itens) e duas para o checklist (seis itens), alcançando Coeficiente de Validade de Conteúdo global de 0,98 e 0,95 respectivamente. Um alfa de Cronbach de 0,79 foi obtido ao final da avaliação. No teste piloto, o cenário foi considerado adequado, com elevados escores de satisfação e autoconfiança na aprendizagem, confirmando a sua usabilidade. Conclusão: o instrumento desenvolvido para avaliação de arritmias chocáveis em pacientes adultos internados em Unidade de Terapia Intensiva pela enfermagem é válido em seu conteúdo e possui boa consistência interna. Objetivo intensiva Método especialistas 3 2021 Gerais literatura 1 brasileiro 08 0 8 0, 800 80 80,0% interna Posteriormente aprendizagem Resultados 21 (2 itens seis itens, , 098 98 0,9 095 95 respectivamente 079 79 0,7 adequado usabilidade Conclusão 202 80,0 2 ( 09 9 07 7 20 80,