Resumen Antecedentes: Existen reportes de que los Hongos Micorrizógenos Arbusculares (HMA) incrementan la producción de metabolitos secundarios en plantas medicinales. El género Croton ha sido poco estudiado en este aspecto. C. draco es una especie etnomedicinal, conocer la relación y naturaleza de los HMA, puede contribuir a obtener plantas en cultivo con mayor calidad y cantidad de metabolitos secundarios. Preguntas: ¿Las raíces de C. draco pueden ser colonizadas por HMA? ¿Qué especies de HMA se encuentran en la rizósfera de C. draco? ¿La diversidad de especies de HMA varía dependiendo del ambiente? Sitios de estudio y periodo de investigación: El estudio se hizo en tres localidades del estado de Veracruz, en el año 2019. Métodos: Raíces terciarias de árboles adultos se procesaron y observaron al microscopio compuesto buscando esporas, hifas y arbúsculos de HMA. La diversidad y densidad esporas en la rizosfera de C. draco, se determinó en muestras de suelo, comparando tres sitios de estudio. Los datos se normalizaron y se hicieron pruebas paramétricas. Resultados: Se observaron esporas, hifas y arbúsculos en las raíces de C. draco. La abundancia de esporas y diversidad de HMA fue diferente entre los sitios evaluados. Con base en la morfología de las esporas se reconocieron 14 taxones, de los cuales, solo dos están presentes en los tres sitios. Conclusión: C. draco presentó en sus raíces estructuras propias de los HMA. Basado en los resultados concluimos que las condiciones ambientales influyen en la abundancia de esporas y en la diversidad de especies de HMA.

Abstract Background: There are reports that Arbuscular Mycorrhizal Fungi (AMF) increase the production of secondary metabolites in medicinal plants. The Croton genus has not been widely studied in this aspect. C. draco is an ethnomedicinal species, knowing the relationship and nature of AMF can contribute to obtaining plants in crops with a good quality and quantity of secondary metabolites. Questions: Can the roots of C. draco be colonized by AMF? What type of AMF species can be found in the rhizosphere of C. draco? Could the diversity of AMF species vary depending on the environment? Study sites and dates: The study was carried out in three locations in the state of Veracruz, in 2019. Methods: Tertiary roots of adult trees were processed and observed under a compound microscope looking for AMF spores, hyphae and arbuscules. The diversity and density of spores in the rhizosphere of C. draco was determined in soil samples, comparing the two sites. Data were normalized and parametric tests were performed. Results: Spores, hyphae and arbuscules were observed on the roots of C. draco. The abundance of spores and AMF diversity was different between the evaluated sites. Based on the morphology of the spores, fourteen taxa were recognized, and among them, only two were found in the three sites. Conclusions: C. draco showed structures typical of AMF in its roots. Based on these results we can say that environmental conditions influence the abundance of spores and the diversity of AMF species.

Abstract The cultivation of avocado (Persea Americana) is not only essential from an economic point of view, but also plays a crucial role in the nutrition, health and well-being of populations around the world. The study was carried out in Ayacucho -Peru (latitude: 12° 53' 57'' S, longitude: 74° 16' 55'' W and altitude: 2596 masl), with the objective of evaluating the effect of the use of the technical drip irrigation system on the production and yield of two varieties of avocado, Hass and Fuerte. The study was based on the use of an irrigation system with programming by treatment with avocado plants installed in plots of 500 m² in 6 sectors, with a density of 5 x 5 m, whose age was between 5 to 16 years, of the which were considered 12 plants (6 plants with drip irrigation and 6 plants as a control with gravity irrigation). Irrigation was carried out right at the flowering and production stage, guaranteeing adequate water supply in periods of water deficit, between February and June 2022. The experimental design was a complete randomized block, with six blocks of 20 plants each. The harvest carried out reported yields of 67.96 ± 8.8 t/ha under technical irrigation and 26.42 ± 4.93 t/ha under gravity irrigation, an increase of 157% with respect to the latter. As for the varieties, the Fuerte avocado had the highest yield 49.38 ± 23.06 t/ha compared to the Hass variety (45.0 ± 21.14 t/ha), the maximum yield occurred with drip irrigation for the Fuerte variety (71.09 ± 8.38 t/ha), this is explained by the energy expenditure in the osmotic adjustment potential which drastically decreases the yield potential with traditional gravity irrigation, obtaining a minimum yield in Hass of 25.18 ± 4.44 t/ha.

Resumen El cultivo de palta (Persea americana) no solo es esencial desde el punto de vista económico, sino que también desempeña un papel crucial en la alimentación, la salud y el bienestar de las poblaciones en todo el mundo. El estudio se realizó en Ayacucho-Perú (latitud: 12° 53’ 57’’ S, longitud: 74° 16’ 55’’ O y altitud: 2596 msnm), con el objetivo de evaluar el efecto de la utilización del sistema de riego tecnificado por goteo en la producción y rendimiento de dos variedades de palta, Hass y Fuerte. El estudio estuvo basado en el uso de un sistema de riego con programación por tratamiento con plantas de palta instaladas en parcelas de 500 m² en 6 sectores, con una densidad de 5 x 5 m, cuya edad estuvo entre 5 a 16 años, de las cuales se consideró 12 plantas (6 plantas con riego por goteo y 6 plantas como testigo con riego por gravedad). Los riegos se efectuaron justo en la etapa de floración y producción garantizando el suministro de agua adecuado en periodos de déficit hídrico, entre febrero a junio de 2022. El diseño experimental fue de bloques completos al azar, con seis bloques de 20 plantas cada uno. La cosecha realizada reporto rendimientos 67,96 ± 8,8 t/ha bajo riego tecnificado y de 26,42 ± 4,93 t/ha en riego por gravedad, siendo un incremento del 157% con respecto a este último. En cuanto a las variedades, la palta Fuerte tuvo el mayor rendimiento 49,38 ± 23,06 t/ha con respecto a la variedad Hass (45,0 ± 21,14 t/ha), el rendimiento máximo se dio con riego por goteo para a variedad Fuerte (71,09 ± 8,38 t/ha), esto es explicado por el gasto de energía en el potencial de ajuste osmótico la cual disminuye drásticamente el potencial de rendimiento con riego tradicional por gravedad, obteniendo un rendimiento mínimo en Hass de 25,18 ± 4,44 t/ha.

Abstract During the distillation process of aromatic plants a liquid byproduct so called hydrolate is produced which is known to have some herbicidal activity in vitro. The next step to study a possible inhibition of germination would be to use the product in greenhouse conditions. With this aim three trials were carried out in big pots using hydrolates of lavender (Lavandula intermedia var. Super) and of Artemisia absinthium at two different rates. As target weed species Echinochloa crus-galli (L.) P. Beauv., Sonchus oleraceus L., Datura stramonium L. and Conyza bonariensis (L.) Cronq. were seeded. The same weed populations were used in the three trials and 4 replicates were conducted. The results showed a more or less pronounced reduction in weed emergence depending on the species already at the lower rates of the hydrolates. The most susceptible species were S. oleraceus and Conyza bonariensis with an emergence reduction between 8 and 100% depending on the trial without a marked difference in the type of hydrolate. E. crus-galli was the least affected by the action of hydrolates. The low emergence of D. stramonium in two of the trials and quite irregular results in the other suggest that more trials should be conducted focusing on analysing some of the factors more in detail such as the irrigation, that could have washed out the bioactive fraction of the hydrolates.

Resumen Durante el proceso de destilación de plantas aromáticas se genera un subproducto líquido llamado hidrolato que, en condiciones in vitro, se sabe que tiene propiedades herbicidas. El siguiente paso para estudiar una posible actividad antigerminante sería utilizar el producto en condiciones de invernadero. Para ello se realizaron tres ensayos, en grandes macetas, utilizando hidrolatos de lavanda (Lavandula intermedia var. Súper) y de Artemisia absinthium a dos dosis diferentes. Como especies diana se sembraron Echinochloa crus-galli (L.) P. Beauv., Sonchus oleraceus L., Datura stramonium L. y Conyza bonariensis (L.) Cronq. En los tres ensayos se utilizaron las mismas poblaciones de arvenses y se hicieron 4 repeticiones por tratamiento. Los resultados mostraron una reducción de la emergencia más o menos acusada en las 4 especies al aplicar ambos hidrolatos ya desde la menor dosis. Las arvenses más sensibles a los hidrolatos fueron S. oleraceus y C. bonariensis con una reducción de la emergencia que osciló entre un 8 y un 100% dependiendo del ensayo sin una marcada diferencia en el tipo de hidrolato. La emergencia de E. crus-galli fue la menos afectada por la acción de los hidrolatos. La baja tasa de emergencia de D. stramonium en dos de los ensayos y los resultados algo irregulares en el otro requieren de una repetición de los ensayos tratando de analizar algunos factores en mayor detalle, como el riego, que pudo haber causado el lavado de la fracción bioactiva del hidrolato.

RESUMO Mutações do gene da periferina (PRPH2) estão associadas à disfunção das células fotorreceptoras e estão envolvidas em várias distrofias retinianas hereditárias. A mutação c.582-1G>A do gene PRPH2 é uma variante rara, relatada na retinite pigmentosa e nas distrofias em padrão. O caso 1 foi de uma mulher de 54 anos com atrofia bilateral do epitélio pigmentar da retina perifoveal e da coriocapilar, com acometimento foveolar central. A autofluorescência e a angiofluoresceinografia revelaram atrofia perifoveal do epitélio pigmentar da retina, com efeito de janela anular, sem o sinal da “coroide escura”. O caso 2 (mãe) apresentava extensa atrofia do epitélio pigmentar da retina e da coriocapilar. Foi feito um estudo do gene PRPH2, que identificou a mutação c.582-1G>A em heterozigose. Foi proposto um diagnóstico de distrofia macular anular concêntrica benigna de início adulto em estágio avançado. A mutação c.582-1G>A é pouco conhecida e não está presente em todos os bancos de dados genômicos usuais. Este é o primeiro relato de caso publicado de uma mutação c.582-1G>A associada à distrofia macular anular concêntrica benigna. PRPH (PRPH2 hereditárias c.5821G>A c5821GA cGA c.582 1G>A c 582 1G G rara padrão 5 coriocapilar central coroide escura. escura . escura” mãe (mãe heterozigose avançado usuais (PRPH 5821G GA c582 c.58 1GA 58 c58 c.5 c5 c.

ABSTRACT The peripherin gene (PRPH2) mutation is associated with photoreceptor cell dysfunction as well as in several inherited retinal dystrophies. The PRPH2 mutation c.582-1G>A is a rare variant reported in retinitis pigmentosa and pattern dystrophy. Here Case 1 was of a 54-year-old woman with bilateral atrophy of the perifoveal retinal pigmentary epithelium and choriocapillaris with central foveolar respect. Autofluorescence and fluorescein angiography revealed perifoveal atrophy of the retinal pigmentary epithelium with an annular window effect without the “dark choroid” sign. Case 2 (mother of Case 1) presented with extensive atrophy of the retinal pigmentary epithelium and choriocapillaris. PRPH2 was evaluated and the c.582-1G>A mutation was identified in heterozygosity. An advanced adult-onset benign concentric annular macular dystrophy diagnosis was thereby proposed. The c.582-1G>A mutation is poorly known and not present in all common genomic databases. This case report is the first one to report a c.582-1G>A mutation associated with benign concentric annular macular dystrophy. PRPH (PRPH2 dystrophies c.5821G>A c5821GA cGA c.582 1G>A c 582 1G A G 54yearold yearold 54 year old respect dark choroid sign mother heterozygosity adultonset adult onset proposed databases (PRPH 5821G GA c582 c.58 1GA 58 5 c58 c.5 c5 c.

Resumo Diante da incerteza quanto à eficácia das vacinas contra a Covid-19 e diante da possibilidade de viver ou morrer, é importante conhecer a percepção e a expectativa de sua aceitação por parte da população. O objetivo principal do presente estudo é analisar a como a vacina contra a Covid-19 é vista pela população estudada e quais são as expectativas que famílias sul-americanas de uma área urbana de Pasco têm em relação a esta vacina. Este é um estudo descritivo, transversal, usando uma amostragem aleatória simples de 197 famílias. Os participantes foram selecionados digitalmente através do contato com uma liderança local e uma pesquisa online foi aplicada com o consentimento prévio dos indivíduos. A análise de regressão logística foi realizada no EPIDAT 4.1 com nível de significância de 5%. Acerca do desejo de se vacinar, é válido enfatizar que há falecimentos de familiares devido ao coronavírus e, portanto, é necessário que a equipe assistencial continue com o trabalho preventivo de incentivo à vacinação para obter resultados favoráveis para toda a população. De acordo com os resultados encontrados no presente estudo, a maioria (100%) dos participantes demonstrou ter uma percepção e expectativa favoráveis sobre a vacina contra a Covid-19 ( X c 2=132,83), com valor-p (0,00). Covid19 Covid 19 Covid-1 morrer sulamericanas sul americanas descritivo transversal indivíduos 41 4 1 4. 5 5% vacinar portanto 100% 100 (100% 2=132,83, 213283 2=132,83 , 2 132 83 2=132,83) valorp valor p 0,00. 000 0,00 . 0 00 (0,00) Covid1 Covid- 10 (100 21328 2=132,8 13 8 0,0 (0,00 (10 2132 2=132, 0, (0,0 (1 213 2=132 (0, 21 2=13 (0 2=1 2=

Abstract Faced with the uncertainty of whether the vaccines against Covid-19 are effective or not and faced with living or dying, it is important to know the perception and expectation of their acceptance. The main aim of the study to analyze the perception and expectation of the vaccine against Covid- 19 that South American families have in an urban area of De Pasco. Descriptive, cross-sectional study, simple random sampling of 197 families. The participants were recruited digitally through a neighborhood leadership and an online survey was applied with prior consent. The logistic regression analysis was performed in EPIDAT 4.1 with a significance level of 5%. Regarding the desire to be vaccinated, it is worth noting that a family member died from the coronavirus, hence the health personnel must continue with the preventive promotional work of vaccination in order to obtain favorable results in the entire population. The majority (100%) have a favorable perception and expectation about the vaccine against Covid- 19 ( X c 2=132.83) and the p-value (0.00); As regards the desire to be vaccinated, it is worth noting having had a family member die from the coronavirus, hence the health personnel must continue with the preventive promotional work of vaccination in order to obtain favorable results in the entire population. Covid19 Covid Covid-1 dying acceptance 1 Pasco Descriptive crosssectional cross sectional consent 41 4 4. 5 5% vaccinated coronavirus population 100% 100 (100% 2=132.83 213283 2 132 83 pvalue p value 0.00 000 0 00 (0.00) Covid1 10 (100 2=132.8 21328 13 8 0.0 (0.00 (10 2=132. 2132 0. (0.0 (1 2=132 213 (0. 2=13 21 (0 2=1 2=

Abstract Background: Members of the genus Cupiennius Simon, 1891 are categorized as wandering spiders and are part of the family Trechaleidae. The genomics and proteomics of Cupiennius spiders from North America remain uncharacterized. The present study explores for the first time molecular data from the endemic species Cupiennius chiapanensis Medina, 2006, and also presents new data for Cupiennius salei (Keyserling, 1878), both collected in southern Mexico. Methods: In total, 88 Cupiennius specimens were collected from southern Mexico and morphologically identified. DNA was extracted and the mitochondrial COI fragment was amplified. COI sequences were analyzed, and a phylogenetic tree was inferred for species from the Americas. Genetic diversity was analyzed using haplotype networks and gene distances. Venom was obtained from C. chiapanensis and C. salei by electrostimulation. The venom was separated by HPLC, visualized using SDS-PAGE, and quantified for use in toxicity bioassays in mice and insects. Results: Analysis of COI sequences from C. chiapanensis showed 94% identity with C. salei, while C. salei exhibited 94-97% identity with sequences from Central and South American conspecifics. The venom from C. chiapanensis exhibited toxic activity against crickets. Venoms from C. chiapanensis and C. salei caused death in Anastrepha obliqua flies. Analysis of venom fractions from C. salei and C. chiapanensis revealed molecular masses of a similar size as some previously reported toxins and neurotoxic components. We determined the amino acid sequences of ChiaTx1 and ChiaTx2, toxins that are reported here for the first time and which showed toxicity against mice and insects. Conclusion: Our work is the first to report COI-based DNA barcoding sequences from southern Mexican Cupiennius spiders. Compounds with toxic activity were identified in venom from both species. Background Simon 189 Trechaleidae uncharacterized Medina 2006 Keyserling, Keyserling (Keyserling 1878, 1878 , 1878) Methods total 8 amplified Americas distances C electrostimulation HPLC SDSPAGE, SDSPAGE SDS PAGE, PAGE SDS-PAGE insects Results 94 9497% 9497 97% 97 94-97 conspecifics crickets flies components ChiaTx ChiaTx2 Conclusion COIbased based 18 200 187 9 949 94-9 1 20 94- 2

Resumen Introducción. Cryptosporidium spp. es un parásito protozoario del grupo de los coccidios, causante de diarreas, trastornos gastroentéricos y respiratorios (baja frecuencia) en diferentes especies de vertebrados a nivel mundial, principalmente en individuos jóvenes. Objetivo. Documentar las características más importantes de virulencia y patogenia del Cryptosporidium spp. así como, la descripción de sus variantes genéticas y su potencial zoonótico. Desarrollo. Existen más de diecinueve especies de Criptosporidium spp., donde se destacan C. parvum (humanos), C. bovis (bovinos), C. suis (porcinos) y C. ubiquitum (aves), debido a su alta capacidad infectiva dada por la diversidad de sus factores de virulencia. El parásito posee afinidad por los enterocitos y genera anomalías en la absorción y funciones secretoras del intestino. La criptosporidiosis varía en función del estado del huésped (edad, nutrición e inmunología), la fuente de contaminación (agua o ingesta), transmisión e higiene. Existen técnicas de diagnóstico, desde pruebas coproscópicas convencionales hasta pruebas de biología molecular. Conclusión. Factores como la variabilidad y los factores de virulencia del Cryptosporidium spp, se consideran mecanismos que favorecen la infección y dispersión del parasito a nivel individual y grupal en humanos y animales domésticos, al igual que la complejidad del ciclo de vida, la resistencia a condiciones ambientales, la capacidad infectiva y la interacción con el huésped consolidan al Cryptosporidium spp. como un agente zoonótico con efectos económicos importantes.

Abstract Introduction. Cryptosporidium spp. is a protozoan parasite belonging to the group of coccidia, causing diarrhea, gastroenteric, and respiratory disorders (less frequently) in different vertebrate species worldwide, primarily in young individuals. Objective. To document the most important virulence and pathogenic characteristics of Cryptosporidium spp., as well as the description of its genetic variants and its zoonotic potential. Development. There are more of 19 species of Cryptosporidium spp., with C. parvum (humans), C. bovis (cattle), C. suis (swine), and C. ubiquitum (birds) being noteworthy due to their high infectivity capacity, given by the diversity of their virulence factors. The parasite has an affinity for enterocytes and causes abnormalities in the intestinal absorption and secretory functions. Cryptosporidiosis varies depending on the host's status (age, nutrition, and immunity), the source of contamination (water or ingestion), transmission, and hygiene. Diagnostic techniques range from conventional coproscopic tests to molecular biology tests. Conclusion. Factors such as the variability and virulence factors of Cryptosporidium spp. are considered mechanisms that favor the infection and spread of the parasite at an individual and group level in humans and domestic animals, as well as the complexity of its life cycle, resistance to environmental conditions, the infective capacity, and host interaction consolidate Cryptosporidium spp. as a zoonotic agent with significant economic effects.

Resumen Objetivo: Evaluar un dispositivo automatizado para la asistencia ventilatoria basado en un AMBU manufacturado en Marzo del 2020. Métodos: El ESSI-1 INC fue evaluado por medio de parámetros fisiológicos y mecánica pulmonar a través de pruebas de espirómetro compensatorios (TISSOT); pulmón artificial (Modelo 5600i Dual Adult PNEU VIEW SYSTEM); así como su desempeño comparado a la máquina de anestesia (AEONMED 7500) en modelos porcinos, midiendo criterios ventilatorios, hemodinámicos y gasométricos. Resultados: Este equipo (ESSI-1 INC) fue exitosamente probado por modelos mecánicos y biológicos, tales como cerdos donde su desempeño fue evaluado en términos de la variabilidad del volumen tidal, frecuencia ventilatoria, y relación I/E versus el desempeño manual de dos médicos. Todos los resultados finalizaron como se esperaba de forma satisfactoria. Conclusiones: Es un equipo seguro y efectivo, el cual debería ser probado y usado en distintas condiciones clínicas para estandarizar la seguridad ventilatoria y cuidado de pacientes que lo requieran.

Abstract Objective: The objectives of this study were to evaluate an automated device for ventilatory support based on AMBU manufactured in March 2020. Methods: The ESSI-1 INC was evaluated through pulmonary mechanics and physiology parameters through compensatory spirometer tests (TISSOTs), and an artificial lung Model5600i Dual Adult PNEU VIEW SYSTEM; it was also compared to the anesthetic ventilatory support equipment (AEONMED 7500) in porcine models, measuring ventilatory, hemodynamic and gasometric parameters. Results: This equipment (ESSI-1 INC) was successfully tested by mechanical and biological models, such as pigs in which its performance was evaluated in terms of variability of tidal volume, ventilation frequency, and I/E relationship versus the manual performance of two medical interns. All the results turned out as expected and were satisfactory. Conclusions: It is safe and effective equipment and should be tested and used in diverse clinical conditions to standardize the ventilatory safety and care of patients who require it.

RESUMO O uso de águas salobras no preparo de soluções nutritivas tem diferentes impactos no desempenho das culturas, dependendo da concentração e prevalência catiônica da água. Portanto, este estudo foi realizado com o objetivo de avaliar a produção e as relações hídricas do coentro cultivado em soluções nutritivas preparadas com águas salobras de diferentes naturezas catiônicas em condições hidropônicas. O delineamento experimental adotado foi de blocos ao acaso, analisado em esquema fatorial 4 × 3, com quatro repetições. Os tratamentos consistiram de quatro níveis de condutividade elétrica da solução nutritiva (1,6; 3,2; 4,8 e 6,4 dS m-1) que foram preparadas em águas salinizadas com diferentes sais (NaCl; CaCl2.2H2O e MgCl2.6H2O). A salinidade afetou negativamente a produção e as relações hídricas do coentro cultivado em sistema hidropônico; verificou-se que as plantas submetidas às soluções nutritivas com predominância de Na+ tiveram maior produção de massa seca total e massa seca da parte aérea, bem como maior gs; assim como a maior área foliar foi obtida utilizando solução nutritiva com predominância de Mg2+. culturas água Portanto hidropônicas acaso 3 repetições 1,6 16 1 6 (1,6 3,2 32 2 48 8 4, 64 6, m1 m m-1 NaCl (NaCl CaCl22H2O CaClHO CaCl2 2H2O CaCl H MgCl2.6H2O. MgCl26H2O MgClHO MgCl2.6H2O . MgCl2 6H2O MgCl MgCl2.6H2O) hidropônico verificouse verificou se Na aérea gs Mg2 Mg Mg2+ 1, (1, m- HO (1 (

ABSTRACT The use of brackish water for preparation of nutrient solutions has several impacts on crop performance, depending on the water concentration and cation prevalence. Therefore, this study was conducted to evaluate production and water relations of coriander grown on nutrient solutions prepared with brackish waters with different cationic natures under hydroponic conditions. A randomized block experimental design with four replicates was used, in a 4 × 3 factorial arrangement. The treatments consisted of four electrical conductivities of the nutrient solution (1.6, 3.2, 4.8, and 6.4 dS m-1) which were prepared in waters with different salts (NaCl, CaCl2.2H2O, and MgCl2.6H2O). Salinity negatively affected the production and water relations of coriander grown in hydroponic system; plants grown on nutritive solutions with predominance of Na+ had higher total dry weight and shoot dry weights, as well as higher stomatal conductance. The largest leaf area was found when using the nutrient solution with predominance of Mg2+. performance prevalence Therefore conditions used arrangement 1.6, 16 1 6 (1.6 32 2 3.2 48 8 4.8 64 6. m1 m m-1 NaCl, NaCl (NaCl CaCl22H2O CaClHO CaCl2 2H2O CaCl H O CaCl2.2H2O MgCl2.6H2O. MgCl26H2O MgClHO MgCl2.6H2O . MgCl2 6H2O MgCl MgCl2.6H2O) system Na weights conductance Mg2 Mg Mg2+ 1.6 (1. 3. 4. m- HO 1. (1 (

RESUMO Leptospirose e toxoplasmose são zoonoses de caráter ocupacional disseminadas mundialmente, principalmente em áreas tropicais e socioeconomicamente vulneráveis. Ambas as doenças têm hospedeiros vertebrados, que espalham o agente infeccioso para o meio ambiente e são potencialmente infecciosos para o homem, as quais podem evoluir para casos fatais. O presente estudo reforça a importância da saúde única para o controle e a profilaxia da leptospirose e da toxoplasmose, mediante a utilização de animais sentinela em áreas de vulnerabilidade socioeconômica, com base em estudo sorológico. Um total de 336 amostras de sangue foi obtido da população canina de um distrito urbano que apresenta altos índices de pobreza e ausência de saneamento básico. Os resultados foram analisados com base em animais sororreagentes e georreferenciados por mapeamento espacial de Kernel. Um total de 43,3% (52/120) dos animais foi sororreagente para anti-Leptospira spp., com predominância de Canicola (35/52 = 67,3%) e Pirogenes (5/52 = 9,6%), enquanto 52,97% (178/336) dos cães foram sororreagentes para anti-Toxoplasma gondii. A presença de cães sororreagentes para ambas as doenças alerta para as condições higiênico-sanitárias inadequadas da região estudada e evidencia a importância de um levantamento da estrutura social, econômica e ambiental para identificar fatores relacionados aos elos da cadeia epidemiológica de transmissão do patógeno e ações corretivas necessárias. mundialmente vulneráveis vertebrados homem fatais socioeconômica sorológico 33 básico Kernel 433 43 3 43,3 52/120 52120 52 120 (52/120 antiLeptospira anti Leptospira spp spp. 35/52 3552 35 (35/5 67,3% 673 67 5/52 552 5 (5/5 9,6%, 96 9,6% , 9 6 9,6%) 5297 97 52,97 178/336 178336 178 (178/336 antiToxoplasma Toxoplasma gondii higiênicosanitárias higiênico sanitárias social necessárias 4 43, 52/12 5212 12 (52/12 35/5 355 (35/ 67,3 5/5 55 (5/ 9,6 529 52,9 178/33 17833 17 (178/33 52/1 521 1 (52/1 35/ (35 67, 5/ (5 9, 52, 178/3 1783 (178/3 52/ (52/ (3 ( 178/ (178/ (52 (178 (17 (1

ABSTRACT Leptospirosis and toxoplasmosis are zoonoses of occupational character that are spread worldwide, mainly in tropical and socioeconomically vulnerable areas. Both diseases have vertebrate hosts, which spread the infectious agent to the environment and are potentially infectious to humans and can progress to fatal cases. The present study reinforces the importance of One Health for the control and prophylaxis of leptospirosis and toxoplasmosis using sentinel animals in areas of socioeconomic vulnerability based on a serological study. A total of 336 blood samples were obtained from the canine population of the urban district, which has high rates of poverty and sanitation. The results were analyzed based on seroreactivity animals and georeferencing by kernel spatial mapping. A total of 43.3% (52/120) of the animals were seroreagents for anti-Leptospira spp., with a predominance of Canicola (35/52 = 67.3%) and Pyrogenes (5/52 = 9.6%), while 52.97% (178/336) of the dogs were seroreactive for anti-Toxoplasma gondii. The presence of seroreagent dogs for both zoonosis alert to the inadequate hygienic-sanitary conditions of the studied region, and highlight the importance of a survey of the social, economic and environmental structure to identify factors related to the links in the epidemiological chain of pathogen transmission and necessary corrective actions. worldwide hosts cases 33 district sanitation mapping 433 43 3 43.3 52/120 52120 52 120 (52/120 antiLeptospira anti Leptospira spp spp. 35/52 3552 35 (35/5 67.3% 673 67 5/52 552 5 (5/5 9.6%, 96 9.6% , 9 6 9.6%) 5297 97 52.97 178/336 178336 178 (178/336 antiToxoplasma Toxoplasma gondii hygienicsanitary hygienic sanitary region social actions 4 43. 52/12 5212 12 (52/12 35/5 355 (35/ 67.3 5/5 55 (5/ 9.6 529 52.9 178/33 17833 17 (178/33 52/1 521 1 (52/1 35/ (35 67. 5/ (5 9. 52. 178/3 1783 (178/3 52/ (52/ (3 ( 178/ (178/ (52 (178 (17 (1

Abstract This paper presents a radiopropagation algorithm based on a Ray Tracing (RT) technique that combines a modified multipath model for constant refractivity gradient profiles and the Uniform Theory of Diffraction (UTD). A novel formulation is proposed by the authors for calculation and ground-reflection analysis of ray paths depending on atmospheric refractivity. The algorithm introduced herein was evaluated in a mixed scenario and in two more realistic case studies, under conditions of constant refractivity gradient and lossy terrain profiles. Pathloss results are obtained and compared with Parabolic Equation (PE) numerical solution results at 2.0 GHz, 3.5 GHz and 5.4 GHz. In such conditions, the modified radiopropagation multipath algorithm with atmospheric refractivity introduced herein showed satisfactory results. RT (RT UTD. UTD . (UTD) groundreflection ground reflection studies PE (PE 20 2 0 2. 35 3 5 3. 54 4 5. (UTD

Resumen Antecedentes. Hasta la fecha, el principal interés clínico de la DPP4 se centra en su inhibición en pacientes diabéticos para prolongar la vida media de las incretinas. Las alteraciones epigenéticas resultantes de la inhibición de DPP4 han sido poco exploradas. Objetivo. Determinar si la sitagliptina, un inhibidor de DPP4, tiene efectos sobre la expresión de KAT7 y SIRT1 (genes que codifican una histona acetiltransferasa y una histona desacetilasa, respectivamente) en células de cáncer de mama MCF7, que desempeñan un papel esencial en la modulación del paisaje epigenético de la cromatina. Método. Las células MCF7 se incubaron durante 20 h con sitagliptina a concentraciones de 0.5, 1.0 y 2.0 μM. Se aisló el ARN total y se determinó la expresión relativa de ARNm de KAT7 y SIRT1 mediante RT-qPCR. Resultados. Hubo una regulación a la baja en la expresión relativa de ambos genes; para KAT7, la regulación negativa alcanzó hasta 0.49 (p = 0.027) y para SIRT1 alcanzó hasta 0.55 (p = 0.037). Conclusiones. Estos resultados sugieren que la sitagliptina tiene efectos sobre el paisaje epigenético de las histonas. Este tema merece más estudios debido al uso actual de inhibidores de DPP4 en pacientes diabéticos.

Abstract Background. To date, the main clinical interest in DPP4 is focused on its inhibition in diabetic patients to prolong the half-life of incretins. Epigenetic alterations resulting from DPP4 inhibition have been poorly explored. Objective. The objective of this study was to determine, whether sitagliptin, a DPP4 inhibitor, has effects on the expression of KAT7 and SIRT1 (genes encoding a histone acetyltransferase and a histone deacetylase, respectively) in MCF7 breast cancer cells, which play an essential role in modulating the epigenetic landscape of chromatin. Material and methods. MCF7 cells were incubated for 20 h with sitagliptin at concentrations of 0.5, 1.0 and 2.0 μM. Total RNA was isolated and the relative mRNA expression of KAT7 and SIRT1 was determined by RT-qPCR. Results. There was downregulation in the relative expression of both genes; for KAT7, downregulation reached up to 0.49 (p = 0.027) and for SIRT1, it reached up to 0.55 (p = 0.037). Conclusions. These results suggest that sitagliptin has effects on the histone epigenetic landscape. This topic deserves further study due to the current sample use of DPP4 inhibitors in diabetic patients.

Abstract Gestational diabetes mellitus (GDM) is a disease with fatal, disabling, and costly consequences for individuals, families, communities and countries. To systematically establish the psychosocial risk factors associated with GDM that should be monitored during and after pregnancy. Systematic review in PubMed/Medline and Cochrane databases. 1188 articles were found and 41 were selected. Some main themes were conflicts with cultural practices, social stigma, ethnicity/race influence, low educational level, sleep quality, breastfeeding problems, concern for one’s own health/baby, low perception of the risk of GDM/DM2 in the future, stress, anxiety and depression, limited knowledge, lack of support from the partner/family/social and health professionals, low levels of self-care/self-efficacy and difficulties with changes in the lifestyle. These factors should be monitored in pregnant women during and after delivery. Treatments should consider the psychological impact and the risk of developing DM2 after childbirth and should be included in clinical practice guidelines. Multi-ethnic populations and the most demographically and socioeconomically vulnerable groups are more susceptible to developing GDM.

Resumen La diabetes mellitus gestacional DMG es una enfermedad con consecuencias mortales, incapacitantes y costosas para las personas, las familias, las comunidades y los países. Con el fin de establecer de manera sistemática los factores de riesgo psicosocial asociados con la DMG que deberían ser monitoreados durante y después del embarazo se realizó una revisión sistemática en las bases de datos PubMed/Medline y Cochrane. Se encontraron 1188 artículos y se seleccionaron 41. Algunos temas principales fueron conflictos con prácticas culturales, estigma social, influencia de la etnia/raza, bajo nivel educativo, calidad del sueño, problemas con la lactancia materna, preocupación por la propia salud o la del bebé, baja percepción de riesgo de DMG o de diabetes mellitus tipo 2 en el futuro, estrés, ansiedad y depresión, conocimiento limitado, falta de apoyo de la pareja, de la familia o social y de los profesionales de la salud, bajos niveles de autocuidado/autoeficacia y dificultades con cambios en el estilo de vida. Estos factores deberían monitorearse en las embarazadas durante y después del parto. Los tratamientos deberían considerar el impacto psicológico y el riesgo de desarrollar diabetes mellitus tipo 2 después del parto, y deberían ser incluidos en las guías de práctica clínica. Las poblaciones multiétnicas y los grupos más vulnerables demográficamente y socioeconómicamente son más susceptibles de desarrollar DMG.

RESUMEN Introducción. Los virus son los principales agentes etiológicos en las infecciones respiratorias agudas graves; un alto porcentaje queda sin diagnóstico viral. Objetivo. Describir la frecuencia de rinovirus y metapneumovirus en pacientes pediátricos de una unidad centinela de Mar del Plata con infección respiratoria aguda grave y resultado negativo para virus clásicos por inmunofluorescencia y biología molecular. Población y métodos. Se realizó un estudio descriptivo de corte transversal. Se evaluó la presencia de rinovirus y metapneumovirus por biología molecular en 163 casos negativos para panel respiratorio por técnicas de vigilancia referencial, durante todo el año 2015. Resultados. Se detectó rinovirus en el 51,5 % de los casos, metapneumovirus en el 9,8 % y coinfección rinovirus-metapneumovirus en el 6,1 %. Fueron negativos para ambos virus el 32,5 %. Conclusiones. La selección de muestras sin diagnóstico virológico permitió identificar rinovirus y metapneumovirus como agentes causales de infecciones respiratorias agudas graves pediátricas y su impacto en la morbimortalidad infantil y en nuestro sistema sanitario.

ABSTRACT The risk analysis evaluates the probability of occurrence with the impact of said risks. In the hydrocarbon sector, an environmental liability is an improperly abandoned facility, soil contaminated by spills or others. The objective of this work is to define a risk management plan. The characterization of the residue and the area was carried out. Hazardous events were identified: overflow spill, soil contamination by infiltration, as well as radiant heat and suffocating and toxic fumes from a fire. The matrix method was applied considering the probability of occurrence against vulnerability. The danger of infiltration implied a high level of risk. The risk management plan was drawn up, its main action being the liquidation of the pool and the sanitation of the area.

RESUMEN El análisis de riesgos evalúa la probabilidad de ocurrencia con el impacto de dichos riesgos. En el sector de hidrocarburos, un pasivo ambiental es una instalación abandonada inadecuadamente, suelo contaminado por derrames u otros. El objetivo de este trabajo es definir un plan de gestión de riesgo. Se realizó la caracterización del residuo y de la zona. Se identificaron los eventos peligrosos: derrame por desborde, contaminación del suelo por infiltración, así como calor por radiación y humos sofocantes y tóxicos de un incendio. Se aplicó el método de las matrices considerando la probabilidad de ocurrencia contra la vulnerabilidad. El peligro de infiltración implicó un nivel alto de riesgo. Se elaboró el plan de gestión de riesgo siendo su principal acción la liquidación de la piscina y el saneamiento del área.