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RESUMO Na agricultura irrigada, é comum a aplicação de água no solo cultivado sem critério, resultando em uso ineficiente ou não otimizado da água. Em muitos casos, a relação solo-água-planta, bem como as potencialidades e limitações dos sistemas de irrigação são ignoradas. A agricultura irrigada sustentável requer o desenvolvimento de um sistema de monitoramento da umidade do solo que evite a perda de água e melhore a eficiência do uso. O objetivo deste estudo foi desenvolver e avaliar a eficiência de um sistema inteligente de monitoramento da umidade do solo. O sistema é composto por duas estações: a primeira coleta os dados sobre parâmetros de umidade aparente do solo usando sensores, enquanto a segunda transmite esses dados para uma estação de processamento central. O sistema determina precisamente os valores atuais de umidade do solo, permitindo estimar o volume de água de irrigação necessário para atender à demanda hídrica da cultura com base na capacidade de campo. Os resultados da curva de calibração dos sensores indicam que o sistema pode medir com precisão a umidade do solo atual, auxiliando no manejo da irrigação. Para áreas irrigadas em condições de solo não saturado, recomenda-se o uso de tensiômetros devido à sua maior confiabilidade entre a capacidade de campo e o ponto de murcha permanente, garantindo práticas de irrigação mais precisas. critério casos soloáguaplanta, soloáguaplanta planta, planta solo-água-planta ignoradas estações central atual saturado recomendase recomenda se permanente precisas

ABSTRACT Water application to cultivated soil is often done without careful consideration in irrigated agriculture, leading to inefficient or suboptimal water usage. In many instances, the intricate relationship between soil, water, and plants, as well as the potential and limitations of irrigation systems, is overlooked. Sustainable irrigated agriculture necessitates the development of a soil moisture monitoring system that curtails water loss and enhances overall efficiency. This study aimed to develop and assess the efficiency of an intelligent system for monitoring soil moisture. The system comprises two stations: the first collects data on apparent soil moisture parameters using sensors, while the second transmits this data to a central processing station. The system precisely determines current soil moisture values, enabling estimation of the required irrigation water volume to meet the crop’s water demand based on field capacity. Results from the calibration curve of the sensors indicate that the system can measure current soil moisture precisely, aiding in irrigation management. For irrigated areas under unsaturated soil conditions, it is recommended to use tensiometers due to their higher reliability between field capacity and permanent wilting point, ensuring more accurate irrigation practices. usage instances plants systems overlooked stations station values crops crop s management conditions point practices

https://doi.org/10.1590/1807-1929/agriambi.v29n2e279856

Respuesta germinativa de Vismia baccifera al tratamiento pregerminativo con ultrasonido
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Lazo Perez, Yamila

; Morales Moreno, Arliet

Revista Cubana de Ciencias Forestales

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dec 2024, Volume 12 N. 3 elocation e858

Abstract: Es Pt En | Text: Es Pt En | PDF: Es | PDF: Pt | PDF: En

RESUMEN En la actualidad orientar estudios sobre tratamientos pregerminativos para acelerar el proceso de germinación de semillas forestales es de gran importancia. La especie Vismia baciifera es muy importante en los programas de reforestación, sin embargo, posee baja capacidad germinativa. El objetivo de este trabajo fue evaluar el efecto del tratamiento pregerminativo con ultrasonido sobre la germinación de semillas de la especie V. baccifera. Se realizó un diseño central compuesto con la metodología de superficie respuesta, mediante el software Design Expert versión 12.0. Se utilizaron 30 semillas con tres réplicas para condición experimental, con el uso de ultrasonido de frecuencia 40 KHz. Las variables consideradas fueron: tiempo del baño ultrasónico (1-10 min), temperatura del agua en el baño (30-60 ºC) y porcentaje de germinación. Los resultados demostraron que el tratamiento pregerminativo con ultrasonido ejerció un efecto positivo en la germinación de semillas de V. baccifera, con un incremento en la germinación de 7.7 % a 67.8 % bajo las condiciones experimentales de 60 ºC y 5.9 min. Esto indica el uso del ultrasonido como tratamiento pregerminativo para el incremento de la germinación de V. baccifera, lo que brinda nuevas alternativas de incursión para especies forestales con bajo potencial germinativo.

RESUMO Atualmente, é de grande importância orientar estudos sobre tratamentos pré-germinativos para acelerar o processo de germinação de sementes florestais. A espécie Vismia baciifera é muito importante em programas de reflorestamento, porém apresenta baixa capacidade germinativa. O objetivo deste trabalho foi avaliar o efeito do tratamento pré-germinativo com ultrassom na germinação de sementes da espécie V. baccifera. Foi realizado um planejamento composto central com a metodologia de superfície de resposta, utilizando o software Design Expert versão 12.0. Para a condição experimental foram utilizadas 30 sementes com três repetições, com uso de ultrassom com frequência de 40 KHz. As variáveis consideradas foram: tempo de banho ultrassônico (1-10 min), temperatura da água do banho (30-60 ºC) e porcentagem de germinação. Os resultados demonstraram que o tratamento pré-germinativo com ultrassom exerceu efeito positivo na germinação de sementes de V. baccifera, com aumento na germinação de 7,7 % para 67,8 % nas condições experimentais de 60 ºC e 5,9 min. Isso indica a utilização do ultrassom como tratamento pré-germinativo para aumentar a germinação de V. baccifera, o que proporciona novas alternativas de incursão para espécies florestais com baixo potencial germinativo.

ABSTRACT Currently, guiding studies on pregerminative treatments to accelerate the germination process of forest seeds is of great importance. The species Vismia baciifera is very important in reforestation programs, however, it has low germination capacity. The objective of this work was to evaluate the effect of pregerminative treatment with ultrasound on the germination of seeds of the species V. baccifera. A central composite design was carried out with the response surface methodology, using Design Expert version 12.0 software. 30 seeds with three replicates were used for the experimental condition, with the use of ultrasound with a frequency of 40 KHz. The variables considered were: ultrasonic bath time (1-10 min), water temperature in the bath (30-60 ºC) and germination percentage. The results demonstrated that the pregerminative treatment with ultrasound exerted a positive effect on the germination of V. baccifera seeds, with an increase in germination from 7.7 % to 67.8 % under the experimental conditions of 60 ºC and 5.9 min. This indicates the use of ultrasound as a pregerminative treatment to increase the germination of V. baccifera, which provides new incursion alternatives for forest species with low germination potential.

Isolation of potential zoonotic Mycobacterium spp. from diseased freshwater angelfish (Pterophyllum scalare) from an aquarium
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Cardoso, P. H. M.

; Moreno, L. Z.

; Ikuta, C. Y.

; Rodrigues, M. V.

; Donola, S. T.

; Heinemann, M. B.

; Balian, S. C.

; Moreno, A. M.

Brazilian Journal of Biology

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2024, Volume 84 elocation e262851

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Resumo A infecção por micobactérias não tuberculosas é uma das doenças bacterianas crônicas mais comuns em peixes ornamentais de aquário e parece estar diretamente relacionada a práticas de manejo estressantes. Além disso, também representa potencial zoonótico. Aqui, apresentamos o isolamento e a caracterização de micobactérias não tuberculosas de peixes-anjo de água doce doentes (Pterophyllum scalare) em São Paulo, Brasil. Foram avaliadas nove fêmeas reprodutoras descartadas com sinais de doença. Os peixes exibiam letargia, perda de apetite, caquexia, úlceras cutâneas e exoftalmia. Na necropsia, quatro peixes apresentaram granulomas macroscópicos no baço. Mycobacterium chelonae, M. fortuitum, M. gordonae, M. intracellulare e M. peregrinum foram isolados e identificados por análise de restrição por PCR de hsp65. A análise histopatológica revelou lesões microscópicas compatíveis com micobacteriose, e o bacilo de Mycobacterium foi observado pela coloração de Ziehl-Neelsen. Notavelmente, todas as espécies de Mycobacterium identificadas neste estudo já foram relatadas em pacientes humanos; portanto, animais doentes podem ser uma fonte de infecção para pessoas que manipulam peixes e aquários.

Abstract Nontuberculous mycobacteria infection is one of the most common chronic bacterial diseases in ornamental aquarium fish and appears to be directly related to stressful husbandry practices. Furthermore, it also represents zoonotic potential. Here we present the isolation and characterization of non-tuberculous mycobacteria from diseased freshwater angelfish (Pterophyllum scalare) in São Paulo, Brazil. Nine discarded breeding females with signs of disease were evaluated. The fish exhibited lethargy, loss of appetite, cachexia, skin ulcers, and exophthalmia. At necropsy, four fishes presented macroscopic granulomas in the spleen. Mycobacterium chelonae, M. fortuitum, M. gordonae, M. intracellulare and M. peregrinum were isolated and identified by hsp65 PCR restriction analysis. Histopathological analysis revealed microscopic lesions compatible with mycobacteriosis, and Mycobacterium bacillus were observed by Ziehl-Neelsen stain. Notably, all Mycobacterium species identified in this study have already been reported in human patients; therefore, diseased animals may be a source of infection for people who handle fish and aquariums.

https://doi.org/10.1590/1519-6984.262851

Comorbilidades asociadas a la mortalidad por COVID-19 en adultos en Lima, Perú: un estudio de cohorte retrospectiva
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Soto-Cabezas, M. Gabriela

; Reyes-Vega, Mary F.

; Soriano-Moreno, Anderson N.

; Ordoñez-Ibargüen, Luis

; Martel, Kevin S.

; Flores-Jaime, Noemi

; Chirinos-Saire, Jenny

; Velásquez, J. Pierre

; Munayco, Cesar V.

Revista Peruana de Medicina Experimental y Salud Pública

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jun 2023, Volume 40 N. 2 Pages 132 - 140

Abstract: Es En | Text: Es En | PDF: Es | PDF: En

RESUMEN Objetivos. Evaluar las comorbilidades asociadas a la mortalidad en pacientes adultos hospitalizados por COVID-19 de diferentes grupos de edad en hospitales de Lima y Callao. Materiales y métodos. En este estudio de cohorte retrospectiva analizamos datos de pacientes adultos hospitalizados por COVID-19, notificados al Sistema Nacional de Vigilancia Epidemiológica del Ministerio de Salud de Perú de marzo a octubre del 2020. Se estimaron riesgos relativos con intervalos de confianza al 95% mediante modelos de regresión de Poisson con varianza robusta para evaluar las comorbilidades asociadas a la mortalidad por grupos de edad: jóvenes (18-29 años), adultos (30-59 años) y mayores (≥60 años). Resultados. Se incluyeron 2366 jóvenes, 23781 adultos y 25356 adultos mayores en el análisis. Los adultos mayores presentaron la mortalidad más alta (63,7%) en comparación con adultos (27,1%) y jóvenes (8,5%). Independientemente del grupo de edad, la presencia de enfermedad neurológica, enfermedad renal, enfermedad hepática y cáncer se asoció a un mayor riesgo de mortalidad. Adicionalmente, la enfermedad cardiovascular fue también un factor de riesgo en los jóvenes; la obesidad, la diabetes, la enfermedad cardiovascular, la enfermedad pulmonar crónica y la inmunodeficiencia en los adultos; y la obesidad y la enfermedad pulmonar crónica en los mayores. Conclusiones: Independientemente de los grupos de edad, los individuos con enfermedad neurológica crónica, enfermedad renal, enfermedad hepática y cáncer tendrían un alto riesgo de morir por la COVID-19.

ABSTRACT Objectives. To evaluate comorbidities associated with mortality in adult patients hospitalized due to COVID-19 in hospitals in Lima and Callao. Materials and methods. In this retrospective cohort study, we analyzed data from adult patients hospitalized due to COVID-19 reported to the National Epidemiological Surveillance System of the Peruvian Ministry of Health from March to October 2020. We estimated relative risks with 95% confidence intervals using Poisson regression models with robust variance to assess comorbidities associated with mortality by age group: young adults (18-29 years), adults (30-59 years) and older adults (≥60 years). Results. We included 2366 young adults, 23,781 adults and 25,356 older adults. Older adults had the highest mortality (63.7%) compared to adults (27.1%) and young adults (8.5%). Regardless of age group, the presence of neurological disease, renal disease, liver disease, and cancer was associated with an increased risk of mortality. Additionally, cardiovascular disease was also a risk factor in young adults; obesity, diabetes, cardiovascular disease, chronic lung disease, and immunodeficiency in adults; and obesity and chronic lung disease in the elderly. Conclusions. Regardless of age groups, individuals with chronic neurologic disease, renal disease, liver disease, and cancer were at high risk of death from COVID-19.

https://doi.org/10.17843/rpmesp.2023.402.12170

Effects of solarization on the biological and chemical properties of a substrate
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Ayoub, i.

; Bigatton, E. D.

; Ortiz, D.

; Archilla, M. V.

; Lucini, E. i.

; Vázquez, C.

; Moreno, M. V.

Agriscientia

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jun 2023, Volume 40 N. 1 Pages 9 - 9

Abstract: En Es | Text: En Es | PDF: En | PDF: Es

ABSTRACT Greenhouse experiments and productions require a high-level control of pests and diseases. Nowadays, society demands to find sustainable alternatives to replace agrochemicals in food production. Solarization is one of the most promising tools due to its low installation cost and control efflciency. The objective of this study was to evaluate the effect of solarization on a substrate for a greenhouse experiment in Córdoba, Argentina. At the INTA-Manfredi Agricultural Experimental Station, 12 m3 of substrate underwent the solarization process, being exposed to solar radiation for six months (December 2021-May 2022). In addition, substrate control remained in natural conditions. Soil functional groups using different growth culture media were measured to verify the solarization effect. The solarized substrate showed a reduction in proteolytic microorganisms (90 %), mesophiles (93 %) and fungi (100 %). The ammonifiers and biological nitrogen fixers microorganisms were reduced by 78 % compared to the control treatment, while yeasts and Bacillus spp. showed reductions up to 64 % and 69 %, respectively. Solarization achieved a 99 % reduction of weeds. The pH decreased by 1.9 % and the electrical conductivity (EC) increased by 5.91 % in the solarized substrate. The total dissolved solids increased by 169 % for the solarized substrate.

RESUMEN Los ensayos y la producción en invernaderos requieren un alto nivel de control de plagas y enfermedades. La sociedad exige alternativas sostenibles que sustituyan a los agroquímicos. La solarización es una de las herramientas prometedoras debido a su bajo costo y eficiencia de control, para la desinfección de sustratos. El objetivo de este estudio fue evaluar el efecto de la solarización sobre un sustrato en Córdoba, Argentina. En la Estación Experimental Agropecuaria INTA-Manfredi, 12 m3 de sustrato se expusieron a la radiación solar durante seis meses (diciembre 2021 - mayo 2022). El sustrato control no fue solarizado. Se midieron grupos funcionales utilizando diferentes medios de cultivo. El sustrato solarizado mostró una reducción de microorganismos proteolíticos (90 %), mesófilos (93 %) y hongos (100 %). Los microorganismos amonificadores y fijadores biológicos de nitrógeno se redujeron en un 78 % en comparación con el tratamiento de control, mientras que las levaduras y Bacillus spp. mostraron reducciones de hasta el 64 % y el 69 %, respectivamente. Las malezas se redujeron 99 %. El pH disminuyó un 1,9 % y la conductividad eléctrica (CE) aumentó un 5,91 % en el sustrato solarizado. Los sólidos disueltos totales (SDT) aumentaron un 169 %.

https://doi.org/10.31047/1668.298x.v1.n40.40311

Comorbilidades asociadas a la mortalidad por COVID-19 en adultos en Lima, Perú: un estudio de cohorte retrospectiva
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Soto-Cabezas, M. Gabriela

; Reyes-Vega, Mary F.

; Soriano-Moreno, Anderson N.

; Ordoñez-Ibargüen, Luis

; Martel, Kevin S.

; Flores-Jaime, Noemi

; Chirinos-Saire, Jenny

; Velásquez, J. Pierre

; Munayco, Cesar V.

Revista Peruana de Medicina Experimental y Salud Publica

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apr 2023, Volume 40 N. 2 Pages 132 - 140

Abstract: En Es | Text: En Es | PDF: En | PDF: Es

https://doi.org/10.17843/rpmesp.2023.402.12170

Study of Melipona quadrifasciata brain under operant learning using proteomic and phosphoproteomic analysis
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HERNÁNDEZ, LIUDY G.

; GARCIA, CARLOS HENRIQUE S.

; SOUZA, JAQUES M.F. DE

; CRUZ, GABRIEL C.N. DA

; CALÁBRIA, LUCIANA KAREN

; MORENO, ANTONIO MAURICIO

; ESPINDOLA, FOUED S.

; SOUZA, DEISY G. DE

; SOUSA, MARCELO V. DE

Anais da Academia Brasileira de Ciências

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2023, Volume 95 Suppl. 1 elocation e20201317

Abstract: En | Text: En | PDF: En

Abstract Learning to anticipate events based on the predictive relationship between an action and an outcome (operant conditioning) is a form of associative learning shared by humans and most of other living beings, including invertebrates. Several behavioral studies on the mechanisms of operant conditioning have included Melipona quadrifasciata, a honey bee that is easily manipulated due to lack of sting. In this work, brain proteomes of Melipona bees trained using operant conditioning and untrained (control) bees were compared by two-dimensional gel electrophoresis analysis within pI range of 3-10 and 4–7; in order to find proteins specifically related to this type of associative learning.One protein was detected with differential protein abundance in the brains of trained bees, when compared to not trained ones, through computational gel imaging and statistical analysis. This protein was identified by peptide mass fingerprinting and MS/MS peptide fragmentation using a MALDI-TOF/TOF mass spectrometer as one isoform of arginine kinase monomer, apparently dephosphorylated. Brain protein maps were obtained by 2-DE (Two-dimensional gel electrophoresis) from a total proteins and phosphoproteins extract of the bee Melipona quadrifasciata. One isoform of arginine kinase, probably a dephosphorylated isoform, was significantly more abundant in the brain of trained bees using operant conditioning. Arginine kinase has been reported as an important enzyme of the energy releasing process in the visual system of the bee, but it may carry out additional and unexpected functions in the bee brain for learning process. beings invertebrates quadrifasciata sting work control (control twodimensional two dimensional 310 3 10 3-1 4–7 47 4 7 learningOne ones MSMS MS MALDITOF/TOF MALDITOFTOF MALDI TOF/TOF TOF monomer 2DE DE 2 Twodimensional Two 31 1 3- 4– MALDITOF TOFTOF

https://doi.org/10.1590/0001-3765202320201317

Sincronización entre la videodeglución y la electromiografía de superficie en pacientes con afectación neurológica y síntomas de disfagia
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Suárez-Patiño, Laura V.

; Orozco-Duque, Andrés

; Pérez-Giraldo, Estefanía

; Roldán-Vasco, Sebastián

; Suárez-Escudero, Juan Camilo

; Martínez-Moreno, Lillyana

Biomédica

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dec 2022, Volume 42 N. 4 Pages 650 - 664

Abstract: Es En | Text: Es En | PDF: Es | PDF: En

Resumen Introducción. La disfagia se define como la dificultad para movilizar la comida desde la boca hasta el estómago. La prueba diagnóstica para esta condición es la videofluoroscopia, la cual no es totalmente inocua pues utiliza radiación ionizante. La electromiografía de superficie registra la actividad eléctrica de los músculos de manera no invasiva, por lo que puede considerarse como una alternativa para evaluar la deglución y estudiar la disfagia. Objetivo. Evaluar la relación entre los tiempos relativos de activación de los músculos implicados en la fase oral y faríngea de la deglución, con los movimientos registrados durante la videofluoroscopia. Materiales y métodos. Se analizaron las señales de la electromiografía de superficie de 10 pacientes neurológicos con síntomas de disfagia, captadas en forma simultánea con la videofluoroscopia. Se suministraron 5 ml de yogur y 10 ml de agua, y 3 g de galleta. Se estudiaron bilateralmente los grupos musculares maseteros, suprahioideos e infrahioideos. Se analizó el paso del bolo por la línea mandibular, las valleculas y el músculo cricofaríngeo, correlacionándolo con el tiempo inicial y el final de la activación de cada uno de los grupos musculares. Resultados. El tiempo promedio de la fase faríngea fue de 0,89 ± 0,12 s. En la mayoría de los casos, hubo activación muscular antes del paso por la línea mandibular y las valleculas. La terminación de la actividad muscular parece corresponder al momento en que se completa el paso del bolo alimenticio por el músculo cricofaríngeo. Conclusión. Se determinaron los tiempos de actividad muscular, la duración de la fase faríngea y la secuencia de la activación de los grupos musculares involucrados en la deglución, mediante electromiografía de superficie, validada con la videofluoroscopia.

Abstract Introduction: Dysphagia is defined as the difficulty in transporting food and liquids from the mouth to the stomach. The gold standard to diagnose this condition is the videofluoroscopic swallowing study. However, it exposes patients to ionizing radiation. Surface electromyography is a non-radioactive alternative for dysphagia evaluation that records muscle electrical activity during swallowing. Objective: To evaluate the relationship between the relative activation times of the muscles involved in the oral and pharyngeal phases of swallowing and the kinematic events detected in the videofluoroscopy. Materials and methods: Electromiographic signals from ten patients with neurological involvement who presented symptoms of dysphagia were analyzed simultaneously with videofluoroscopy. Patients were given 5 ml of yogurt, 10 ml of water, and 3 g of crackers. Masseter, suprahyoid, and infrahyoid muscle groups were studied bilaterally. The bolus transit through the mandibular line, vallecula, and the cricopharyngeus muscle was analyzed in relation to the onset and offset times of each muscle group activation. Results: The average time of the pharyngeal phase was 0.89 ± 0.12 s. Muscle activation was mostly observed prior to the bolus transit through the mandibular line and vallecula. The end of the muscle activity suggested that the passage of the bolus through the cricopharyngeus muscle was almost complete. Conclusión: The muscle activity times, duration of the pharyngeal phase, and sequence of the muscle groups involved in swallowing were determined using sEMG validated with the videofluoroscopic swallowing study.

https://doi.org/10.7705/biomedica.6446

[SciELO Preprints] - Guideline of the Brazilian Society of Cardiology on Diagnosis and Treatment of Patients with Chagas Disease Cardiomyopathy
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Marin-Neto, José Antonio Rassi Jr., Anis Moraes Oliveira, Gláucia M. Lemos Correia, Luís Claudio Novaes Ramos Jr., Alberto Hasslocher-Moreno, Alejandro Marcel Luquetti Ostermayer, Alejandro Sousa, Andréa Silvestre de Amato Vincenzo de Paola, Angelo Sobral de Sousa, Antonio Carlos Pinho Ribeiro, Antonio Luiz Correia Filho, Dalmo Moraes de Souza, Dilma do Socorro Cunha-Neto, Edecio J. A. Ramires, Felix Bacal, Fernando Pereira Nunes, Maria do Carmo Martinelli Filho, Martino Ibrahim Scanavacca, Maurício Magalhães Saraiva, Roberto Alves de Oliveira Júnior, Wilson M. Lorga-Filho, Adalberto de Jesus Benevides de Almeida Guimarães, Adriana Lopes Latado Braga, Adriana Sarmento de Oliveira, Adriana V. L. Sarabanda, Alvaro Yecê das Neves Pinto, Ana Assis Lopes do Carmo, André Schmidt, André Costa, Andréa Rodrigues da Ianni, Barbara Maria Markman Filho, Brivaldo Eduardo Rochitte, Carlos Thé Macedo, Carolina Mady, Charles Chevillard, Christophe Bittencourt das Virgens, Cláudio Marcelo Nery de Castro, Cleudson De Paoli de Carvalho Britto, Constança Felícia Pisani, Cristiano do Carmo Rassi, Daniela C. Sobral Filho, Dario Rodrigues Almeida, Dirceu A. Bocchi, Edimar T. Mesquita, Evandro de Souza Nogueira Sardinha Mendes, Fernanda Pereira, Francisca Tatiana Sperandio da Silva, Gilberto Marcelo de Lima Peixoto, Giselle Glotz de Lima, Gustavo H. Veloso, Henrique Turin Moreira, Henrique Bellotti Lopes, Hugo Masciarelli Francisco Pinto, Ibraim Pinto Dias, João Carlos Bemfica, João Marcos Silva-Nunes, João Paulo Soares Barreto-Filho, José Augusto Kerr Saraiva, José Francisco Lannes-Vieira, Joseli Menezes Oliveira, Joselina Luzia V. Armaganijan, Luciana Martins, Luiz Cláudio C. Sangenis, Luiz Henrique Barbosa, Marco Paulo Almeida-Santos, Marcos Antônio Simões, Marcos Vinicius Shikanai-Yasuda, Maria Aparecida Vieira Moreira, Maria da Consolação Higuchi, Maria de Lourdes Costa Monteiro, Maria Rita de Cássia Felix Mediano, Mauro Felippe Maia Lima, Mayara T. Oliveira, Maykon Moreira Dias Romano , Minna Nitz, Nadjar de Tarso Jorge Medeiros, Paulo Vieira Alves, Renato Alkmim Teixeira, Ricardo Coury Pedrosa, Roberto Aras, Roque Morais Torres, Rosália dos Santos Povoa, Rui Manoel Rassi, Sérgio Gabriel Salles Xavier, Sérgio Marinho Martins Alves , Silvia B. N. Tavares, Suelene Lima Palmeira, Swamy da Silva Junior, Telêmaco Luiz da Rocha Rodrigues, Thiago Madrini Junior, Vagner Maia da Costa , Veruska Dutra, Walderez

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Publication date: 2022-10-11

Abstract: En Pt

This guideline aimed to update the concepts and formulate the standards of conduct and scientific evidence that support them, regarding the diagnosis and treatment of the Cardiomyopathy of Chagas disease, with special emphasis on the rationality base that supported it.nbsp; Chagas disease in the 21st century maintains an epidemiological pattern of endemicity in 21 Latin American countries. Researchers and managers from endemic and non-endemic countries point to the need to adopt comprehensive public health policies to effectively control the interhuman transmission of T. cruzi infection, and to obtain an optimized level of care for already infected individuals, focusing on diagnostic and therapeutic opportunistic opportunities. nbsp; Pathogenic and pathophysiological mechanisms of the Cardiomyopathy of Chagas disease were revisited after in-depth updating and the notion that necrosis and fibrosis are stimulated by tissue parasitic persistence and adverse immune reaction, as fundamental mechanisms, assisted by autonomic and microvascular disorders, was well established. Some of them have recently formed potential targets of therapies.nbsp; The natural history of the acute and chronic phases was reviewed, with enhancement for oral transmission, indeterminate form and chronic syndromes. Recent meta-analyses of observational studies have estimated the risk of evolution from acute and indeterminate forms and mortality after chronic cardiomyopathy. Therapeutic approaches applicable to individuals with Indeterminate form of Chagas disease were specifically addressed. All methods to detect structural and/or functional alterations with various cardiac imaging techniques were also reviewed, with recommendations for use in various clinical scenarios. Mortality risk stratification based on the Rassi score, with recent studies of its application, was complemented by methods that detect myocardial fibrosis.nbsp; The current methodology for etiological diagnosis and the consequent implications of trypanonomic treatment deserved a comprehensive and in-depth approach. Also the treatment of patients at risk or with heart failure, arrhythmias and thromboembolic events, based on pharmacological and complementary resources, received special attention. Additional chapters supported the conducts applicable to several special contexts, including t. cruzi/HIV co-infection, risk during surgeries, in pregnant women, in the reactivation of infection after heart transplantation, and others.nbsp; nbsp;nbsp; Finally, two chapters of great social significance, addressing the structuring of specialized services to care for individuals with the Cardiomyopathy of Chagas disease, and reviewing the concepts of severe heart disease and its medical-labor implications completed this guideline.

Esta diretriz teve como objetivo principal atualizar os conceitos e formular as normas de conduta e evidências científicas que as suportam, quanto ao diagnóstico e tratamento da CDC, com especial ênfase na base de racionalidade que a embasou. A DC no século XXI mantém padrão epidemiológico de endemicidade em 21 países da América Latina. Investigadores e gestores de países endêmicos e não endêmicos indigitam a necessidade de se adotarem políticas abrangentes, de saúde pública, para controle eficaz da transmissão inter-humanos da infecção pelo T. cruzi, e obter-se nível otimizado de atendimento aos indivíduos já infectados, com foco em oportunização diagnóstica e terapêutica. Mecanismos patogênicos e fisiopatológicos da CDC foram revisitados após atualização aprofundada e ficou bem consolidada a noção de que necrose e fibrose sejam estimuladas pela persistência parasitária tissular e reação imune adversa, como mecanismos fundamentais, coadjuvados por distúrbios autonômicos e microvasculares. Alguns deles recentemente constituíram alvos potenciais de terapêuticas. A história natural das fases aguda e crônica foi revista, com realce para a transmissão oral, a forma indeterminada e as síndromes crônicas. Metanálises recentes de estudos observacionais estimaram o risco de evolução a partir das formas aguda e indeterminada e de mortalidade após instalação da cardiomiopatia crônica. Condutas terapêuticas aplicáveis aos indivíduos com a FIDC foram abordadas especificamente. Todos os métodos para detectar alterações estruturais e/ou funcionais com variadas técnicas de imageamento cardíaco também foram revisados, com recomendações de uso nos vários cenários clínicos. Estratificação de risco de mortalidade fundamentada no escore de Rassi, com estudos recentes de sua aplicação, foi complementada por métodos que detectam fibrose miocárdica. A metodologia atual para diagnóstico etiológico e as consequentes implicações do tratamento tripanossomicida mereceram enfoque abrangente e aprofundado. Também o tratamento de pacientes em risco ou com insuficiência cardíaca, arritmias e eventos tromboembólicos, baseado em recursos farmacológicos e complementares, recebeu especial atenção. Capítulos suplementares subsidiaram as condutas aplicáveis a diversos contextos especiais, entre eles o da co-infecção por T. cruzi/HIV, risco durante cirurgias, em grávidas, na reativação da infecção após transplante cardíacos, e outros.nbsp;nbsp;nbsp; Por fim, dois capítulos de grande significado social, abordando a estruturação de serviços especializados para atendimento aos indivíduos com a CDC, e revisando os conceitos de cardiopatia grave e suas implicações médico-trabalhistas completaram esta diretriz.nbsp;

https://doi.org/10.1590/SciELOPreprints.4820

10.

Extracorporeal Membrane Oxygenation (ECMO) Training Program in A Pediatric Cardiac Intensive Care Unit: An 8-Year Single-Center Experience in Argentina
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Pilan, María Luisa

; Krynski, Mariela

; Moreno, Guillermo Eduardo

; Ponce, Gisela M.

; Rodríguez, Ricardo E.

; Domínguez, Estela R.

; Cornelis, Carlos Javier

; Montonati, Maria Mercedes

; Lenz, Ana Miriam

; Desocio, Maria Belen

; Bravo, Nélida N.

; Vassallo, Juan Carlos

; Santos, Silvia N.

; Rodríguez, Gabriela V.

; Cordisco, Barbara

; Althabe, Maria J.

Brazilian Journal of Cardiovascular Surgery

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oct 2022, Volume 37 N. 5 Pages 654 - 662

Abstract: En | Text: En | PDF: En

ABSTRACT Introduction: Extracorporeal membrane oxygenation (ECMO) for temporary cardiopulmonary support is one of the most intense and technologically complex therapies offered in medicine. It is a high-risk procedure that requires specific knowledge and technical skills to perform it with good results. Objective: The main goal of this study is to describe our extracorporeal membrane oxygenation (ECMO) training program based on the study of specialized nurses and physicians of a simulation teaching experience, conducted in a pediatric cardiac intensive care unit. The program was developed as a theoretical-practical course with final exam and annual maintenance training sessions, caring for ECMO patients, its implementation and results. Methods: A descriptive study for registered nurses, intensivists, and cardiac surgeons. A self-administered, anonymous, and voluntary survey was conducted to assess the long-term perception about the program. Demographic data to describe the population was required, and questions about satisfaction and confidence in acquired skills and competences were asked. A descriptive statistical analysis was performed; patient survival and complications were compared before and after ECMO program using chi-square test, and P<0.05 was considered statistically significant. Results: Twenty-four training courses were performed for 68 professionals. More than 88% of the professionals considered the course components to be adequate and complete; and 94% felt trained to manage the ECMO circuit. Most valued activities were workshops and clinical cases. Since the implementation of the training program, 88 patients were assisted, with a survival rate at discharge of 58%, higher than in the previous period (P=0.03). Conclusion: More than 80% of the professionals considered the workshops and simulations as the most useful components. Reliance on the circuit care was higher than in training problem scenarios. Since 2013 we assisted 88 patients on ECMO, with a survival rate at discharge of 58%, within international standards results.

https://doi.org/10.21470/1678-9741-2021-0560

11.

Extracts of Moringa oleifera and Croton californicus against infections of Vibrio parahaemolyticus (IPNGS16) in juvenile Pacific white shrimp (Penaeus vannamei)
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Lugo-Rubio, Jesús Yolanda

; López-Álvarez, Ely Sara

; Vázquez-Montoya, Nadia

; Escamilla-Montes, Ruth

; Félix-Ortiz, José Adán

; Lugo-Medina, Eder

; Herrera-Moreno, María Nancy

; Nava-Pérez, Eusebio

; Valenzuela-Quiñónez, Wenceslao

Latin american journal of aquatic research

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sep 2022, Volume 50 N. 4 Pages 541 - 552

Abstract: En | Text: En | PDF: En

ABSTRACT We evaluated the inhibition activity of extracts from two common plants, moringa (Moringa oleifera) and croton (Croton californicus), against Vibrio parahaemolyticus (IPNGS16), which causes acute hepatopancreatic necrosis disease (AHPND). The experiment was developed in three phases. First, extracts were prepared, and phytochemical screening of plants was performed. Second, microbiological tests were applied to calculate the minimum inhibitory concentration (MIC). Finally, two bioassays were performed on juvenile shrimp by administering the extracts (first) in shrimp feed at 24 and 72 h, pre-infection, and the second in feed and directly to the culture water. Based on preliminary results of antimicrobial activity, an extract concentration of 60 mg mL-1 inhibited V. parahaemolyticus (IPNGS16) at a final concentration of approximately 1×106 CFU mL-1 . Bioassays were carried out in order to determine the V. parahaemolyticus (IPNGS16) median lethal dose (LC50) for juvenile Penaeus vannamei (0.20 ± 0.05 g); the LC50 was 85×103 CFU mL-1. As a first test, the extracts were added to commercial feed at doses of 20, 40, and 60 mg mL-1. After feeding, the shrimp were infected with V. parahaemolyticus (IPNGS16), and mortality was recorded. For the first infection time (24 h), survival was 46 and 33% with croton and moringa, respectively; for the second infection time (72 h), survival was 16 and 25% with croton and moringa. Application of antibacterial extracts directly to culture water were effective against V. parahaemolyticus (IPNGS16). The best result (94% survival) was obtained with a high dose (3.6 mg mL-1 or 30%) of moringa extracts added directly to culture water. Moringa methanol extracts produce active compounds capable of inhibiting replication of V. parahaemolyticus (IPNGS16) in shrimp aquaculture and reducing shrimp mortality.

https://doi.org/10.3856/vol50-issue4-fulltext-2825 104 downloads

12.

Diferencias en los componentes biométricos oculares entre hombres y mujeres
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Rey-Rodríguez, Diana V.

; Álvarez-Peregrina, Cristina

; Moreno-Montoya, José

; Ramírez-Arcos, Diana P.

; Tovar-Oviedo, Fernanda L.

Revista mexicana de oftalmología

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aug 2022, Volume 96 N. 4 Pages 155 - 161

Abstract: Es En | Text: Es En | PDF: Es | PDF: En

Resumen Objetivo: Evaluar las diferencias en los componentes biométricos oculares, como longitud axial, profundidad de la cámara anterior, espesor del cristalino, curvatura corneal y espesor corneal entre hombres y mujeres. Método: Estudio observacional analítico de corte transversal, en 106 estudiantes universitarios en edades entre 18 y 36 años. Todos los sujetos fueron sometidos a refracción bajo cicloplejia y valorados mediante autorrefractometría. El error refractivo fue medido después de la aplicación de ciclopentolato al 1%. Para la identificación de los componentes biométricos se realizó una valoración con el equipo Aladdin (Topcon Corporation, Equipos Oftalmológicos Colombia). Resultados: De los 106 participantes, el 71.7% (76) correspondían al sexo femenino, en edades entre 18 y 36 años. En las mujeres, el promedio de la longitud axial fue de 23.3 ± 0.88 mm y en los hombres 23.9 ± 0.99 mm. La profundidad de la cámara anterior fue de 3.5 ± 0.26 mm en las mujeres y de 3.6 ± 0.22 mm en los hombres. El espesor del cristalino en las mujeres fue de 3.47 ± 0.22 mm y en los hombres de 3.53 ± 0.20 mm. El espesor corneal en las mujeres fue de 538.65 ± 0.36 μm y en los hombres de 542.43 ± 32.73 μm. El promedio de la queratometría en las mujeres fue de 43.54 ± 1.35 D y en hombres de 42.76 ± 1.79 D. Hubo diferencias estadísticamente significativas entre la curvatura corneal (-0.78 D; hombres p = 0.01 y mujeres p = 0.003) y la longitud axial (0.63 mm; hombres p = 0.02 y mujeres p = 0.04). Conclusiones: Existen diferencias en la curvatura corneal de hasta 0.75 D, siendo más curva en las mujeres, y un mayor tamaño del globo ocular en los hombres, características anatómicas que intervienen en la prevalencia de errores de refracción.

Abstract Purpose: To evaluate the differences in ocular biometric components such as axial length, anterior chamber depth, lens thickness, corneal curvature and corneal thickness between men and women. Method: Cross-sectional analytical observational study in 106 university students aged 18-36 years. All subjects underwent refraction under cycloplegia and assessed through autorefractometry. The refractive error was measured after the application of 1% cyclopentolate. For the identification of biometric components, an assessment was carried out with the Aladdin equipment (Topcon Corporation, Equipos Oftalmológicos Colombia). Results: Of the 106 participants, 71.7% (76) corresponded to the female sex, aged between 18 to 36 years. In women, the mean axial length was 23.3 ± 0.88 mm and in men 23.9 ± 0.99 mm. The anterior chamber depth was 3.5 ± 0.26 mm in women and 3.6 ± 0.22 mm in men. The lens thickness in women was 3.47 ± 0.22 thickness in womenm and 3.53 ± 0.20 μm in men. Corneal thickness in women was 538.65 ± 0.36 μm and in men it was 542.43 ± 32.73 μm. The mean keratometry in women was 43.54 ± 1.35 D and in men 42.76 ± 1.79 D. There were statistically significant differences between corneal curvature (-0.78 D; for men p = 0.01 and for women p = 0.003) and axial length (0.63 mm; for men p = 0.02 and for women p = 0.04). Conclusions: There are differences in corneal curvature of up to 0.75 D, being more curved in women and a larger size of the eyeball in men, anatomical characteristics that intervene in the prevalence of refractive errors.

https://doi.org/10.24875/rmo.m22000238

13.

Un análisis de la jurisprudencia reciente del Tribunal Europeo de Derechos Humanos en materia de inmigración irregular por ingresos clandestinos
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Hellman Moreno, Jacqueline

Revista de derecho (Valdivia)

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jun 2022, Volume 35 N. 1 Pages 217 - 236

Abstract: En Es | Text: En Es | PDF: En | PDF: Es

ABSTRACT: In Europe, migratory movements have reached, in this new century, very high figures that have led to the implementation of controversial State measures. This, of course, has entailed the opening of many legal proceedings before the European Court of Human Rights. Consequently, we will analyze the sentence N.D. and N.T. v. Spain according to which we will conclude that the aforementioned judicial body has begun a regressive turn in the matter of the rights of migrants, distancing itself from the provisions previously made in the case Hirsi Jamaa et al. v. Italy. In this context, the case S.S. and others v. Italy will be brought, since it will allow us to anticipate the main considerations regarding the future ruling. Likewise, it should be indicated that in light of part of the facts surrounding this last matter, we will highlight the possibility of sustaining the pertinent international responsibility; this is not a trivial question as it could reverse the change of the course recently undertaken.

RESUMEN Los movimientos migratorios en Europa han alcanzado en este nuevo siglo cifras muy altas que han venido acompañadas de la implementación de controvertidas medidas estatales. Ello, como es lógico, ha provocado la apertura de numerosos procesos judiciales ante el Tribunal Europeo de Derechos Humanos. Consecuentemente, analizaremos el fallo N.D. y N.T. c. España conforme al cual concluiremos que el citado órgano judicial ha iniciado un giro regresivo en materia de derechos de las personas migrantes, distanciándose de lo dispuesto con anterioridad en el asunto Hirsi Jamaa y otros c. Italia. En este contexto, se traerá a colación el caso S.S. y otros c. Italia, porque nos permitirá anticipar las principales consideraciones en torno al futuro fallo. Asimismo, debe indicarse que a la luz de parte de los hechos que rodean a este último asunto pondremos de relieve la posibilidad de sostener la responsabilidad internacional pertinente; esta no es una cuestión baladí, ya que podría revertir el cambio de rumbo recientemente emprendido.

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14.

Experiencia en esplenopancreatectomías distales laparoscópicas
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Chiacchio, María V.

; Gorini Ditchoff, Giselle S.E.

; Garelli, Andrés

; Cilla, Eliana G.

; Moreno Negri, José M.

; Peso, Santiago M.

; Rossi, Javier L.

Revista argentina de cirugía

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jun 2022, Volume 114 N. 2 Pages 124 - 132

Abstract: Es En | Text: Es En | PDF: Es | PDF: En

RESUMEN Antecedentes: la resección pancreática distal es una técnica quirúrgica utilizada para el abordaje de lesiones del páncreas izquierdo. Objetivo: Analizar los resultados de la experiencia en esplenopancreatectomías distales laparoscópicas de nuestro hospital, a fin de evaluar los resultados y beneficios obtenidos. Material y métodos: se realizó un estudio retrospectivo y observacional de 17 pacientes intervenidos desde el año 2015 hasta el año 2020. Resultados: el 47,10% fueron hombres (n=8) y el 52,90% mujeres (n=9). Los motivos de consulta más frecuentes fueron: incidentaloma 52,94% (n=9) y dolor abdominal 48,06% (n=8). Se utilizaron como estudios de hallazgo más frecuentes la ecografía abdominal y la tomografía con contraste, que se complemetaron con otros estudios de alta complejidad. Entre los diagnósticos preoperatorios, el 11,76% (n=2) fue NQS, el 47,06% (n=8) NQM, el 5,88% NPM (n=1) y pseudoquiste pancreático, 11,76% (n=2), tumor maligno y 17,66% (n=3) TNE. El tamaño tumoral promedio fue de 4,26 cm (rango 2-10 cm). La tasa de conversión fue 23,08% (n=3). La media del tiempo operatorio fue de 270 minutos. Las complicaciones observadas fueron subdivididas en menores y mayores. Presentamos dos reinternaciones y no se informaron casos de mortalidad ni reoperaciones. Discusión: la laparoscopia es un abordaje seguro y eficaz para patología benigna y maligna pancreática en casos seleccionados. La fuga bioquímica, la fístula pancreática y la tasa de morbilidad presentan un valor similar al de otras series publicadas de resecciones pancreáticas laparoscópicas. No obtuvimos diferencias significativas entre el tratamiento del muñón pancreático y el riesgo de fístula.

ABSTRACT Background: Distal pancreatectomy with or without splenectomy is a surgical technique to approach lesions of the left pancreas. Objective: To analyze our experience in laparoscopic distal pancreatosplenectomies with evaluation of results and benefits. Material and methods: We performed a retrospective and observational study of 17 patients operated on from 2017 to 2020. Results: 47.10% were men (n = 8) and 52.90% were women (n = 9). The most common reasons for consultation were incidental tumors in 52.94% (n = 9); abdominal pain in 48.06% (n = 8). The diagnostic imaging tests performed were abdominal ultrasound and computed tomography scan of the abdomen and were complemented with other high-complexity studies. The preoperative diagnoses were SCN (11.76%; n = 2), MCN (5.88%; n = 1), pancreatic pseudocyst (11,76%; n = 2); malignant tumor and NET (17.66; n = 3). The mean size of the tumors was 4.26 cm (range 2-10 cm). Conversion rate was 23.08% (n = 3). Median operative time was 270 minutes. The complications observed were subdivided in minor and major. Two patients were readmitted and there were no cases or mortality or reoperations. Discussion: Laparoscopy is a safe and efficient approach for benign and malignant tumor s of the pancreas in selected cases. Biochemical leak, pancreatic fistula and morbidity rate are similar to those reported by other series of laparoscopic pancreatic resections. We did not find significant differences between the treatment of the pancreatic stump and the risk of fistula.

https://doi.org/10.25132/raac.v114.n2.1633 49 downloads

15.

Factors Associated with Development of Acute Kidney Injury After Liver Transplantation
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Catalán, Rodrigo

; Jiménez-Ceja, José V.

; Rincón-Pedrero, Rodolfo

; Olivas-Martínez, Antonio

; Martínez-Rueda, Armando J.

; Bazúa-Valenti, Silvana

; Carrillo-Pérez, Diego L.

; Grajeda-Medina, Leoneli I.

; García-Juárez, Ignacio

; Vilatobá, Mario

; Ortega-Trejo, Juan A.

; Pérez-Villalva, Rosalba

; Bobadilla, Norma A.

; Moreno, Erika

; Gamba, Gerardo

Revista de investigación clínica

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apr 2022, Volume 74 N. 2 Pages 90 - 96

Abstract: En | Text: En | PDF: En

ABSTRACT Background: Early post-liver transplant (LT) acute kidney injury (AKI) has been associated with worse short-term and long-term outcomes, but the incidence and risk factors in our population are unknown. Methods: We designed a prospective, single-center, longitudinal cohort study to determine the incidence of AKI during the immediate postoperative period of LT, and to identify the risk factors associated with AKI after LT. Pre-operative and intraoperative variables were analyzed to determine if there was any correlation with the development of post-operative AKI. Results: Eighty-six patients were included in the final analysis; from them, 45 (52%) developed AKI in the following 30 days after LT. The presence of hepatic encephalopathy prior to LT was the factor most strongly associated with the development of AKI (Relative Risk 3.67, 95% Confidence Interval 1.08-8.95). Other factors associated with AKI development were male gender and a higher serum lactate during surgery. Conclusion: AKI was a frequent complication that significantly worsened the prognosis of LT recipients and was associated with an increased 30-day mortality rate. The presence of hepatic encephalopathy strongly predicted the development of severe AKI.

https://doi.org/10.24875/ric.21000496 30 downloads

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