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au:Meneses, Paulo Roberto

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Morphological and biochemical characterization of bacterial species of Bacillus, Lysinibacillus and Brevibacillus Bacillus
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Rocha, Gabriela Teodoro

; Montalvão, Sandro Coelho Linhares

; Queiroz, Paulo Roberto Martins

; Berçot, Marcelo Rodrigues

; Gomes, Ana Cristina Meneses Mendes

; Monnerat, Rose Gomes

Revista Ceres

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jun 2023, Volume 70 N. 3 Pages 91 - 104

Abstract: En | Text: En | PDF: En

ABSTRACT The objective of this work was to characterize reference bacteria strains, belonging to the genus Bacillus and species of correlated genera, by simplified morphological and biochemical methods. The morphological characterization is based on the aspects of the colonies, as well as cytomorphology of the species, by optical and scanning microscopy. For biochemical characterization, the sensitivity test to antimicrobials by disk-diffusion is performed. Moreover, the strains were characterized by extracting intracellular proteins. Characteristics such as shape, color, and consistency of the colonies, in addition to the type of spore and production of protein crystals were determinants for the morphological characterization of these species. The antibiogram revealed high resistance to β-lactam group antibiotics, in species of Bacillus cereus s.l group. In Bacillus subtilis s.l. group there was high susceptibility to antibiograms, mainly for species of B. subtilis. The protein profile provided specific protein patterns for some species, mainly bands of 130 e 65 kDa for B. thuringiensis, 140 e 130 kDa for Lysinibacillus sphaericus, and 115 kDa for Brevibacillus laterosporus. Our results showed that the morphological and biochemical characterizations, provided a simple identification, with easy interpretation, and low cost. genera methods colonies microscopy diskdiffusion disk diffusion performed Moreover proteins shape color βlactam β lactam antibiotics sl s l antibiograms B 13 6 thuringiensis 14 sphaericus 11 laterosporus characterizations identification interpretation cost 1

https://doi.org/10.1590/0034-737x202370030010

Death-related factors in HIV/AIDS patients undergoing hemodialysis in an intensive care unit
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Cavalcante, Malena Gadelha

; Parente, Matheus de Sá Roriz

; Gomes, Pedro Eduardo Andrade de Carvalho

; Meneses, Gdayllon Cavalcante

; Silva Júnior, Geraldo Bezerra da

; Pires Neto, Roberto da Justa

; Daher, Elizabeth De Francesco

Revista do Instituto de Medicina Tropical de São Paulo

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2021, Volume 63 elocation e33

Abstract: En | Text: En | PDF: En

ABSTRACT HIV-infected patients are at high risk for developing critical diseases, including opportunistic infections (OI), with consequent admission in intensive care units (ICU). Renal disfunctions are risk factors for death in HIV/AIDS patients, and survival rates in patients undergoing hemodialysis are smaller than the ones observed in the general population. In this context, this study aimed to investigate death-related factors in HIV/AIDS patients in an intensive care setting. This is a retrospective cross-sectional study performed through the analysis of medical records from 271 HIV/AIDS-diagnosed patients hospitalized in an intensive care unit of an infectious disease hospital, in Fortaleza, Ceara State, Brazil. Patients were divided into two groups: those who underwent dialysis during hospitalization and those who did not. Clinical and demographic parameters that could be associated with death were evaluated. Results indicated a prevalence of death of 19.1% (CI 95%: 14.8-24.3). The median age of patients was 47 years, with a male predominance (71.3%). The main causes of admission were pulmonary tuberculosis (16.9%), followed by neurotoxoplasmosis (14.9%). In the bivariate analysis, for those that did not undergo dialysis, age, fever, dyspnea, oliguria, disorientation, kidney injury, use of lamivudine and efavirenz, length of hospitalization, CD4 count, WBC count, platelet count, urea, sodium and LDH levels were the associated variables. In those who needed dialysis, the use of stavudine, abacavir and ritonavir, and the length of hospitalization were associated factors. Renal toxicity by the antiretroviral agents and length of hospitalization increased the risk of death among HIV patients under dialysis.

https://doi.org/10.1590/s1678-9946202163033 399 downloads

RECOMMENDATIONS FOR INVASIVE PROCEDURES IN PATIENTS WITH DISEASES OF THE LIVER AND BILIARY TRACT: REPORT OF A JOINT MEETING OF THE BRAZILIAN SOCIETY OF HEPATOLOGY (SBH), BRAZILIAN SOCIETY OF DIGESTIVE ENDOSCOPY (SOBED) AND BRAZILIAN SOCIETY OF INTERVENTIONAL RADIOLOGY AND ENDOVASCULAR SURGERY (SOBRICE)
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SCHIAVON, Leonardo de Lucca

; EJIMA, Flávio Hayato

; MENEZES, Marcos Roberto de

; BITTENCOURT, Paulo Lisboa

; MOREIRA, Aírton Mota

; FARIAS, Alberto Queiroz

; CHAGAS, Aline Lopes

; ASSIS, André Moreira de

; MATTOS, Ângelo Zambam de

; SALOMÃO, Bruno Chaves

; TERRA, Carlos

; MARTINS, Fernanda Prata Borges

; CARNEVALE, Francisco Cesar

; REZENDE, Guilherme Ferreira da Motta

; PAULO, Gustavo Andrade de

; PEREIRA, Gustavo Henrique Santos

; MOTTA-LEAL-FILHO, Joaquim Maurício da

; MENESES, Juliana de

; COSTA, Lucas Santana Nova da

; CARNEIRO, Marcos de Vasconcelos

; ÁLVARES-DA-SILVA, Mário Reis

; SOARES, Mayra Veloso Ayrimoraes

; PEREIRA, Osvaldo Ignácio

; XIMENES, Rafael Oliveira

; DURANTE, Renata Filardi Simiqueli

; FERREIRA, Valério Alves

; LIMA, Vinícius Machado de

Arquivos de Gastroenterologia

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jun 2019, Volume 56 N. 2 Pages 213 - 231

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO As doenças do fígado e das vias biliares são causas comuns de morbidade e mortalidade. Procedimentos invasivos com finalidade diagnóstica e terapêutica são frequentemente recomendados nos casos de doenças hepatobiliares. O reconhecimento das indicações e limitações das técnicas comumente empregadas é crucial para uma adequada seleção dos pacientes, maximizando os resultados positivos e reduzindo o risco de complicações. Em 2018, a Sociedade Brasileira de Hepatologia (SBH), em colaboração com a Sociedade Brasileira de Radiologia Intervencionista e Cirurgia Endovascular (SOBRICE) e a Sociedade Brasileira de Endoscopia Digestiva (SOBED) realizaram um encontro exclusivamente voltado para a discussão dos procedimentos invasivos nas doenças hepatobiliares. Este texto resume as principais recomendações discutidas durante o evento, e tem a intenção de auxiliar clínicos, gastroenterologistas, hepatologistas, radiologistas e endoscopistas no uso adequado dos procedimentos invasivos para manejo de pacientes com doenças hepatobiliares.

ABSTRACT Liver and biliary tract diseases are common causes of morbidity and mortality worldwide. Invasive procedures are usually performed in those patients with hepatobiliary diseases for both diagnostic and therapeutic purposes. Defining proper indications and restraints of commonly used techniques is crucial for proper patient selection, maximizing positive results and limiting complications. In 2018, the Brazilian Society of Hepatology (SBH) in cooperation with the Brazilian Society of Interventional Radiology and Endovascular surgery (SOBRICE) and the Brazilian Society of Digestive Endoscopy (SOBED) sponsored a joint single-topic meeting on invasive procedures in patients with hepatobiliary diseases. This paper summarizes the proceedings of the aforementioned meeting. It is intended to guide clinicians, gastroenterologists, hepatologists, radiologists, and endoscopists for the proper use of invasive procedures for management of patients with hepatobiliary diseases.

https://doi.org/10.1590/s0004-2803.201900000-42 2181 downloads

Correção atmosférica e de "crosstalk" em dados Aster para mapeamento da relação mineralógica de solos
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Baptista, Gustavo Macedo de Mello

; Vivaldi, Daniella Dias

; Meneses, Paulo Roberto

Pesquisa Agropecuária Brasileira

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sep 2016, Volume 51 N. 9 Pages 1272 - 1282

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Resumo O objetivo deste trabalho foi avaliar a influência das correções atmosférica e de "crosstalk" (vazamento de radiação entre detectores) da qualidade das imagens RCGbscale, em dados SWIR do Aster, sobre a identificação da relação mineralógica RCGb [caulinita/(caulinita + gibbsita)], em uma mancha de solos expostos derivados do Complexo de Niquelândia, composto por gabronoritos, noritos e piroxenitos. Utilizaram-se dados brutos L1A, L1B (radiância no topo da atmosfera), produtos AST07 (reflectância de superfície sem correção de "crosstalk") e AST07XT (reflectância de superfície com correção de "crosstalk"), os módulos de correção atmosférica Flaash e Quac, e o programa de correção "crosstalk" CRSTK30. Os resultados foram ordenados de acordo com os seguintes parâmetros: os transectos RCGbscale e RCGbATG; a identificação da espacialização da relação mineralógica nas imagens RCGbscale, fatiadas em classes de valor Ki; e a verificação quantitativa do grau de similaridade entre os valores dos 17 pontos dos transectos RCGbscale e RCGbATG, por meio dos testes estatísticos t de Student e U de Mann-Whitney. Os procedimentos apresentaram 75% de resultados satisfatórios para o estudo proposto. Os dados L1B, bem como os efeitos atmosféricos e os do "crosstalk", não interferem na identificação da relação RCGb. Estudos em outros solos devem ser incentivados para melhor compreensão da interferência dessas correções nos dados Aster.

Abstract The objective of this work was to evaluate the effect of atmospheric and crosstalk (the electrical or optical signals of band leaks into another band) corrections of RCGbscale image quality, in Aster SWIR data, on the identification of the mineralogical relationship RCGb [kaolinite/(kaolinite + gibbsite)], in a spot of bare soils derived from the Niquelândia Complex, composed by gabbronorites, norites and pyroxenites. Raw data were used for L1A, L1B (radiance at the top of the atmosphere), AST07 (surface reflectance without crosstalk correction) and AST07XT (surface reflectance with crosstalk correction) on-demand products, the atmospheric correction modules Flaash and Quac, and the CRSTK30 crosstalk correction program. The results were sorted according to the following parameters: the transects RCGbscale and RCGbATG; the identification of the spatialization of the mineralogical relationship in the RCGbscale images, which were sliced into Ki value classes; and the quantitative check of the similarity degree between the values of 17 points of the RCGbscale and RCGbATG transects, by Student's t and Mann-Whitney U statistical tests. The procedures showed 75% satisfactory results for the proposed study. The L1B data, as well as the atmospheric and crosstalk effects, do not interfere in the identification of the RCGb relationship. Studies in other soils should be stimulated for a better understanding of the interference of these corrections in Aster data.

https://doi.org/10.1590/s0100-204x2016000900027 1443 downloads

LEPROSY NEPHROPATHY: A REVIEW OF CLINICAL AND HISTOPATHOLOGICAL FEATURES
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Silva Junior, Geraldo Bezerra da

; Daher, Elizabeth De Francesco

; Pires NETO, Roberto da Justa

; Pereira, Eanes Delgado Barros

; Meneses, Gdayllon Cavalcante

; Araújo, Sônia Maria Holanda Almeida

; Barros, Elvino José Guardão

Revista do Instituto de Medicina Tropical de São Paulo

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feb 2015, Volume 57 N. 1 Pages 15 - 20

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

A hanseníase é doença crônica causada pelo Mycobacterium leprae, altamente incapacitante e com envolvimento sistêmico em alguns casos. O envolvimento renal tem sido relatado em todas as formas da doença, sendo mais frequente nas formas multibacilares. A apresentação clínica é variável e determinada pela reação do sistema imunológico do hospedeiro ao bacilo. Durante o curso da doença podem ocorrer os chamados estados reacionais, nos quais o sistema imune reage contra o bacilo, exacerbando as manifestações clínicas. Diferentes lesões renais tem sido descritas na hanseníase, incluindo glomerulonefrites, nefrite intersticial, amiloidose secundária e pielonefrite. O mecanismo exato que leva à glomerulonefrite na hanseníase ainda não está completamente esclarecido. O tratamento da hanseníase inclui o uso de rifampicina, dapsona e clofazimina. Prednisona e antiinflamatórios não-hormonais podem ser usados no controle dos episódios imunológicos agudos.

Leprosy is a chronic disease caused by Mycobacterium leprae, highly incapacitating, and with systemic involvement in some cases. Renal involvement has been reported in all forms of the disease, and it is more frequent in multibacillary forms. The clinical presentation is variable and is determined by the host immunologic system reaction to the bacilli. During the course of the disease there are the so called reactional states, in which the immune system reacts against the bacilli, exacerbating the clinical manifestations. Different renal lesions have been described in leprosy, including acute and chronic glomerulonephritis, interstitial nephritis, secondary amyloidosis and pyelonephritis. The exact mechanism that leads to glomerulonephritis in leprosy is not completely understood. Leprosy treatment includes rifampicin, dapsone and clofazimine. Prednisone and non-steroidal anti-inflammatory drugs may be used to control acute immunological episodes.

https://doi.org/10.1590/S0036-46652015000100002 4815 downloads

Development of a subcutaneous endometriosis rat model
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Pereira, Francisco Edson Ximenes Gomes

; Almeida, Paulo Roberto Carvalho de

; Dias, Bruno Hállan Meneses

; Vasconcelos, Paulo Roberto Leitão de

; Guimarães, Sérgio Botelho

; Medeiros, Francisco das Chagas

Acta Cirúrgica Brasileira

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jan 2015, Volume 30 N. 1 Pages 6 - 12

Abstract: En | Text: En | PDF: En

PURPOSE:To present a rat model of subcutaneous endometriosis for the study of pathophysiology and the effects of drugs.METHODS: Fifty three-month-old female Wistar rats (Rattus norvergicus) were distributed into one control group and four treatment groups: estradiol (2.5; 5; 10mg/kg sc), medroxyprogesterone acetate (0.5; 2; 5mg/kg sc), triptorelin pamoate (0.18; 0.56mg/kg sc) and acetylsalicylic acid (3mg/kg per os). The animals were autoimplanted subcutaneously with 4x4-mm uterine fragments to induce endometriosis. The endometriomas were measured on days 1, 7, 14 and 21. The relative dry and wet weights of the endometrioma were used to evaluate response to the drug. Endometrial -like tissue was confirmed by histology. The greatest weight gain was observed on day 14 (relative wet weight: 29.1 ± 6.7mg%, relative dry weight: 5.3 ± 0.9mg %). Treatments were administered between day 5 and day 14.RESULTS:The relative wet weight of the hemiuterus in the 10mg/kg estradiol group differed significantly from control and the other two estradiol groups (p=0.0001). In the medroxyprogesterone acetate group the weight decreased significantly but this decrease was not dose-dependent. Weight reduction was also significant in the triptorelin pamoate and the acetylsalicylic acid groups.CONCLUSION:The model of subcutaneous endometriosis is reproducible, low-cost and easy to perform, and suitable for the study of pathophysiology and the effects of drugs.

https://doi.org/10.1590/S0102-86502015001000002 3697 downloads

Abordagem metodológica baseada em imagens do SAR-R99B para identificar prováveis pistas de pouso não-homologadas na Amazônia
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Alves, Sergio Alexandre Saloio

; Assante, Luis Ricardo

; Sano, Edson Eyji

; Meneses, Paulo Roberto

Acta Amazonica

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sep 2009, Volume 39 N. 3 Pages 727 - 729

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Este estudo apresenta uma abordagem metodológica baseada em imagens de radar e nos critérios de tonalidade, tamanho e forma geométrica para identificar prováveis pistas de pouso não-homologadas na Amazônia. Os seguintes procedimentos foram conduzidos: georreferenciamento da imagem do sensor SAR-R99B do município paraense de Itaituba, adquirida na banda L, polarização HH e resolução espacial de três metros; subtração do ruído speckle com filtro mediana; classificação com a técnica não-supervisionada ISODATA; vetorização da classe indicativa dos alvos de interesse; e cálculo e seleção automática dos alvos de interesse por critérios de índice de circularidade e de tortuosidade. Foram identificados dez alvos, dos quais dois foram considerados como prováveis pistas de pouso pelos referidos índices.

This study presents a methodological approach based on radar images and on gray level, size and geometric pattern to identify potential unofficial airplane strips in Amazonia. The following procedures were conducted: georeferencing of a L-band, HH polarization, 3-meter spatial resolution image obtained by the SAR-R99B system over the Itaituba municipality, State of Para; subtraction of speckle noise with a median filter; image classification with the ISODATA unsupervised technique; conversion of selected targets of interest into vector format; and calculation and automatic selection of targets of interest by the circularity and tortuosity indices. Among the 10 previously identified targets, two were considered as potential unofficial airplane strips by the above mentioned indices.

https://doi.org/10.1590/S0044-59672009000300031 2164 downloads

Análise de dados hiperespectrais em mina de manganês laterítico, São João da Aliança (GO)
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Carvalho Júnior, Osmar Abílio de

; Ribeiro, Múcio Nobre da Costa

; Martins, Éder de Souza

; Guimarães, Edi Mendes

; Meneses, Paulo Roberto

; Guimarães, Renato Fontes

Revista Brasileira de Geofísica

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jun 2007, Volume 25 N. 2 Pages 199 - 212

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

O objetivo do presente trabalho foi desenvolver uma metodologia para identificação de lateritas manganesíferas por espectroscopia de imageamento. Nesse estudo foi utilizada a imagem do Airborne Visible/InfraRed Imaging Spectrometer (AVIRIS). O AVIRIS é um sensor que mede o espectro solar refletido de 400 nm para 2500 nm em intervalos de 10 nm. A área de estudo localiza-se no Estado de Goiás e engloba a mina Fazenda Extrema que apresenta depósitos de manganês desenvolvido nas rochas do Grupo Paranoá. Estudos de espectrometria de laboratório, conjugado com análise mineralógica por difratometria de raios-X, demonstram o caráter opaco do espectro do minério que não apresentam feições de absorção distintas devido à banda de condução do MnO3 que se estende por todo o intervalo espectral. Visando explorar essa característica foi desenvolvida uma metodologia para o processamento digital de imagem contendo três etapas: (a) correção atmosférica pelo método Green, (b) seleção das áreas com comportamento opaco pelo cálculo da integral do espectro, e (c) individualização das áreas com presença de lateritas manganesíferas entre os alvos de baixo albedo utilizando a transformação por Minimum Noise Fraction. O procedimento desenvolvido mostrou ser eficiente na classificação de áreas de mina de manganês abandonadas que consistem em áreas prioritárias para recuperação ambiental.

The aim this paper is to develop a methodology of lateritic manganese identification using imaging spectroscopy. In this study was used the Airborne Visible/InfraRed Imaging Spectrometer (AVIRIS) image. The AVIRIS is an imaging sensor that measures the solar reflected spectrum from 400 nm to 2500 nm at 10 nm intervals. The study area is located at Goiás State and enclosed the Fazenda Extrema mine that presents lateritic manganese deposits developed on Paranoá Group rocks. Laboratory spectrometry studies conjugated with mineralogical analysis by diffractometry of X-ray demonstrated that the ore spectrum is opaque and spectrally featureless, due to the conduction band of MnO3 extending throughout this spectral range. The digital image processing can be subdivided in the following steps: (a) atmospheric correction using Green method, (b) areas selection with opaque behavior from the integral spectra calculation, and (c) lateritic manganese individualization using the Minimum Noise Fraction transformation. The method is efficient to classify areas of abandoned manganese mine that consist of priority areas for implementation of environmental recovery.

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Ressonância magnética funcional na determinação da lateralização da área cerebral da linguagem
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Meneses, Murilo S.

; Rocha, Samanta F. Blattes

; Blood, Marcelo R. Young

; Trentin Jr., Alcides

; Benites Filho, Paulo Roberto

; Kowacs, Pedro André

; Oliveira, Nelson de Andrade

; Simão, Cristiane A.

; Awamura, Yumi

; Vítola, Maria L.A.

Arquivos de Neuro-Psiquiatria

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mar 2004, Volume 62 N. 1 Pages 61 - 67

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

A ressonância magnética funcional (RMF) é uma nova técnica capaz de detectar pequenas alterações no fluxo sanguíneo e oxigenação de tecidos cerebrais em que ocorre ativação neuronal. O seu emprego na avaliação pré-cirúrgica de pacientes com epilepsia portadores de esclerose mesial temporal está atualmente em avaliação em alguns centros de neurologia. O principal objetivo é encontrar o melhor paradigma de ativação na avaliação das funções de linguagem e memória, visando a substituição do teste de Wada, largamente utilizado nos dias de hoje. Para formular um paradigma já adaptado ao nosso idioma, apresentamos a nossa experiência em uma tarefa comportamental de fluência verbal na determinação da lateralização da área cerebral da linguagem. Avaliaram-se os exames de RMF de uma clínica de imagem particular em Curitiba por período de aproximadamente dois anos. Dos 19 pacientes estudados, obteve-se sucesso no exame em 16 e, destes, todos apresentavam dominância hemisférica cerebral da linguagem à esquerda. Em um subgrupo com 5 pacientes foi possível comparar os resultados obtidos com a técnica de Wada e RMF, havendo concordância entre as técnicas. A partir deste estudo e de vários outros semelhantes na literatura, acreditamos que a RMF está progressivamente conquistando seu espaço na prática médica.

Functional magnetic resonance imaging (fMRI) is a technique for detecting minimal changes in brain perfusion and oxygenation secondary to neuronal activation. Its application in the pre-surgical evaluation of epileptic patients with temporal mesial sclerosis is currently being under investigation in several centers. This study aims to describe an activation paradigm for the evaluation of language and memory functions, as an alternative to the worldwide used Wada test, which is an invasive procedure. In order to propose a paradigm adapted to the Portuguese language, we report our experience in determining the dominant cerebral area for language through fMRI with a verbal fluency task. The results of the fMRI from 19 patients studied in Curitiba in a period of approximately two years were studied. Sixteen of them presented with left hemispheric cerebral language dominance. In five patients, results from fMRI and Wada test could be compared and agreed in localization. Our results reinforce the view that fMRI may become an essential tool for medical practice, perhaps for the determination of eloquent areas in the evaluation of candidates for epilepsy surgery.

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