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Predictive factors for recording the death of women of childbearing age in the Hospital Information System (SIH/SUS), Brazil, 2012–2020
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Marques, Juliana Alves

; Domingues, Rosa Maria Soares Madeira

; Dias, Marcos Augusto Bastos

; Coeli, Claudia Medina

; Pinheiro, Rejane Sobrinho

; Saraceni, Valeria

Revista Brasileira de Epidemiologia

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2024, Volume 27 elocation e240051

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO Objetivo: Estimar a cobertura de registro de óbito de mulheres em idade fértil (MIF) no Sistema de Informação Hospitalar (SIH), segundo hospital de ocorrência, e verificar os preditores associados à cobertura. Métodos: Estudo ecológico descritivo com dados públicos do SIH, Sistema de Informação sobre Mortalidade (SIM) e Cadastro Nacional de Estabelecimentos de Saúde (CNES) no período 2012–2020. Óbitos em internações de MIF no SIH foram comparados aos do SIM. Calculou-se a cobertura pela proporção de óbitos no SIH em relação ao SIM. Modelos de classificação supervisionada — árvore de decisão e floresta aleatória — foram utilizados para identificação das características hospitalares relacionadas à cobertura. Resultados: A cobertura de registro de óbito de MIF foi estimada em 78%, e em 71,8% após exclusão de hospitais com cobertura >100%. Menor cobertura foi observada na região Norte (67,7%) e maior na Sul (76,9%). Houve aumento da cobertura — de 69% para 74,4% — no período. Os principais fatores preditivos da cobertura foram instalação física de urgência/emergência, esfera administrativa, nível de complexidade, proporção de leitos adultos conveniados ao SUS e atividade de ensino, com menor cobertura naqueles com instalação de urgência/emergência e maior cobertura naqueles com maior complexidade, da esfera federal, com atividade de ensino e maior proporção de leitos adultos conveniados ao SUS. Falhas no registro do CNES foram identificadas no SIM. Conclusão: A cobertura de registro de óbito de MIF no SIH no país é elevada e crescente. Diferenças regionais reforçam a necessidade de estratégias de melhoria da qualidade dos sistemas de informação.

ABSTRACT Objective: To estimate the death registration coverage of women of childbearing age (WCA) in the Hospital Information System (SIH), according to the hospital of occurrence and to verify the predictors associated with coverage. Methods: Descriptive ecological study with public data from SIH, Mortality Information System (SIM) and National Registry of Health Establishments (CNES), 2012–2020. Deaths in WCA hospitalizations in SIH were compared to those in SIM. Coverage was calculated by the proportion of deaths in SIH in relation to SIM. Supervised classification models — decision tree and random forest — were used to identify hospital characteristics related to coverage. Results: WCA death registration coverage was estimated at 78.0 and 71.8% after excluding hospitals with >100% coverage. Lower coverage was observed in the North region (67.7%) and higher in the South (76.9%). There was an increase in coverage from 69.0% to 74.4% in the period examined. The main factors predicting coverage were urgency/emergency facility, administrative management level, hospital complexity, proportion of adult beds covered by SUS and teaching activity, with lower coverage in those with an urgency/emergency facility and greater coverage in those of higher complexity, in federal hospitals, those with teaching activity and higher proportion of adult beds covered by SUS. Flaws in the CNES registration were identified in SIM. Conclusion: The coverage of WCA death registration in SIH in the country is high and growing. Regional differences reinforce the need for strategies to improve the quality of information systems.

https://doi.org/10.1590/1980-549720240051

Proposal for the applicability of modified Breslow (measured from the basal membrane) as a predictor of survival and sentinel lymph node outcome in patients with cutaneous melanoma measured membrane
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Asato, Marcel Arakaki

; Moares Neto, Francisco Alves

; Moraes, Marcelo Padovani de Toledo

; Ocanha-Xavier, Juliana Polizel

; Takita, Luiz Carlos

; Marques, Mariangela Esther Alencar

; Xavier-Júnior, José Cândido Caldeira

Anais Brasileiros de Dermatologia

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2024, Volume 99 N. 3 Pages 398 - 406

Abstract: En | Text: En | PDF: En

Abstract Background Cutaneous melanoma is a neoplasm with a high mortality rate and risk of metastases to distant organs. The Breslow micrometric measurement is considered the most important factor for evaluating prognosis and management, measured from the granular layer to the deepest portion of the neoplasm. Despite its widespread use, the Breslow thickness measurement has some inaccuracies, such as not considering variations in the thickness of the epidermis in different body locations or when there is ulceration. Objective To evaluate the applicability of a modified Breslow measurement, measured from the basal membrane instead of from the granular layer, in an attempt to predict sentinel lymph node examination outcome and survival of patients with melanoma. Methods A retrospective and cross-sectional analysis was carried out based on the evaluation of slides stained with hematoxylin & eosin from 275 cases of melanoma that underwent sentinel lymph node biopsy from 2008 to 2021 at a reference center in Brazil. Results Analysis of the Cox model to evaluate the impact of the Breslow measurement and the modified Breslow measurement on survival showed that both methods are statistically significant. Logistic regression revealed a significant association between both measurements and the presence of metastasis in sentinel lymph nodes. Conclusion Measuring melanoma depth from the basal membrane (modified Breslow measurement) is capable of predicting survival time and sentinel lymph node outcome, as well as the conventional Breslow measurement. organs management use inaccuracies ulceration crosssectional cross sectional 27 200 202 Brazil nodes 2 20

https://doi.org/10.1016/j.abd.2023.09.002

Predictive factors for recording the death of women of childbearing age in the Hospital Information System (SIH/SUS), Brazil, 2012–2020 SIH/SUS, SIHSUS SIH/SUS , SIH SUS (SIH/SUS) Brazil 20122020 2012 2020 2012–202 (SIH/SUS 2012202 201 202 2012–20 201220 20 2012–2 20122 2 2012–
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Marques, Juliana Alves

; Domingues, Rosa Maria Soares Madeira

; Dias, Marcos Augusto Bastos

; Coeli, Claudia Medina

; Pinheiro, Rejane Sobrinho

; Saraceni, Valeria

Revista Brasileira de Epidemiologia

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2024, Volume 27 elocation e240051

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

https://doi.org/10.1590/1980-549720240051

Saúde mental na atenção básica: percepção dos Agentes Comunitários de Saúde básica
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Salgado, Jéssica Walesca

; Zanela, Letícia Rocha Marques

; Rocha, Lívia Toledo

; Calmeto, Marina Nakao

; Meneguetti, Juliana Alves

; Vidal, Carlos Eduardo Leal

Cadernos Saúde Coletiva

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2023, Volume 31 N. 2 elocation e31020065

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Resumo Introdução Os fluxos de encaminhamento dos pacientes portadores de transtornos mentais e o seu devido acolhimento representam um grande desafio na área da saúde mental. Objetivo Conhecer a percepção dos Agentes Comunitários de Saúde em relação aos usuários da saúde mental e suas atividades nesse campo. Método Estudo de ordem qualitativa utilizando a técnica de grupo focal. Os dados coletados foram transcritos e submetidos à Técnica de Análise Qualitativa de Conteúdo. Participaram do estudo 15 agentes de saúde. Resultados Os dados foram organizados utilizando os temas que se destacaram nos depoimentos dos Agentes Comunitários de Saúde e agrupados em três categorias selecionadas previamente: Conhecimento sobre Transtornos Mentais, Ações em Saúde Mental, Entraves dos Serviços. Conclusão Os agentes demonstraram interesse pelo trabalho com os pacientes, mas apontaram dificuldades envolvendo os diversos pontos da rede de atenção à saúde. Melhor preparação técnica e teórica dos agentes resultaria em ganhos para a equipe de saúde e para os pacientes. Apesar de contemplar a realidade de um município de médio porte, este estudo pode fornecer subsídios para a discussão sobre a assistência em saúde mental na atenção básica de saúde. campo focal Conteúdo 1 previamente Mentais Mental Serviços porte

Abstract Background The referral flows of patients with mental disorders and their proper reception represent a major challenge in the mental health area. Objective To know the perception of Community Health Agents concerning mental health patients and their activities in this field. Method This is a qualitative study using the focus group technique. The collected data were transcribed and submitted to the Qualitative Content Analysis Technique. Fifteen health agents participated in the study. Results The data were organized using the themes that stood out in the testimonies of the Community Health Agents and grouped into three previously selected categories: Knowledge about Mental Disorders, Mental Health Actions, and Service Barriers. Conclusion The agents showed interest in working with patients but pointed out difficulties involving the different points of the healthcare network. Better technical and theoretical preparation of agents would result in gains for the health team and for patients. Despite considering the reality of a medium-sized municipality, this study can provide support for the discussion on mental health care in primary health care. area field technique Technique categories Disorders Actions Barriers network mediumsized medium sized municipality

https://doi.org/10.1590/1414-462x202331020065

Chikungunya virus infection in the southernmost state of Brazil was characterised by self-limited transmission (2017-2019) and a larger 2021 outbreak selflimited self limited 20172019 2017 2019 (2017-2019 202 2017201 201 (2017-201 20 201720 (2017-20 2 20172 (2017-2 (2017- (2017 (201 (20 (2 (
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Gregianini, Tatiana Schäffer

; Salvato, Richard Steiner

; Barcellos, Regina Bones

; Godinho, Fernanda Marques

; Ruivo, Amanda Pellenz

; de Melo, Viviane Horn

; Schroder, Júlio Augusto

; Martiny, Fernanda Letícia

; Möllmann, Erica Bortoli

; Favreto, Cátia

; Baethgen, Ludmila Fiorenzano

; Ferreira, Vithoria Pompermaier

; de Lima, Lívia Eidt

; Piazza, Cláudia Fasolo

; Machado, Taís Raquel Marcon

; Becker, Irina Marieta

; Ramos, Raquel Rocha

; Frölich, Guilherme Carey

; Rossetti, Alana Fraga

; Almeida, Lucas da Cunha

; Rodrigues, Tahiana Machado Antunes

; Bragança, Isabella Tabelli

; Campos, Aline Alves Scarpellini

; Manzoni, Verônica Baú

; Machado, Lais Ceschini

; da Silva, Luisa Maria Inácio

; de Oliveira, André Luiz Sá

; Paiva, Marcelo Henrique Santos

; Nunes, Zenaida Marion Alves

; de Almeida, Paula Rodrigues

; Demoliner, Meriane

; Gularte, Juliana Schons

; da Silva, Mariana Soares

; Filippi, Micheli

; Pereira, Vyctoria Malayhka de Abreu Góes

; Spilki, Fernando Rosado

; da Veiga, Ana Beatriz Gorini

; Wallau, Gabriel Luz

Memórias do Instituto Oswaldo Cruz

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2023, Volume 118 elocation e220259

Abstract: En | Text: En | PDF: En

BACKGROUND Chikungunya is a mosquito-borne virus that has been causing large outbreaks in the Americas since 2014. In Brazil, Asian-Caribbean (AC) and East-Central-South-African (ECSA) genotypes have been detected and lead to large outbreaks in several Brazilian states. In Rio Grande do Sul (RS), the southernmost state of Brazil, the first cases were reported in 2016. OBJECTIVES AND METHODS We employed genome sequencing and epidemiological investigation to characterise the Chikungunya fever (CHIKF) burden in RS between 2017-2021. FINDINGS We detected an increasing CHIKF burden linked to travel associated introductions and communitary transmission of distinct lineages of the ECSA genotype during this period. MAIN CONCLUSIONS Until 2020, CHIKV introductions were most travel associated and transmission was limited. Then, in 2021, the largest outbreak occurred in the state associated with the introduction of a new ECSA lineage. CHIKV outbreaks are likely to occur in the near future due to abundant competent vectors and a susceptible population, exposing more than 11 million inhabitants to an increasing infection risk. mosquitoborne mosquito borne 2014 Brazil AsianCaribbean Asian Caribbean AC (AC EastCentralSouthAfrican East Central South African (ECSA states RS, , (RS) 2016 (CHIKF 20172021. 20172021 2017 2021. 2021 2017-2021 period 2020 limited Then lineage population 1 risk 201 (RS 2017202 202 2017-202 20 201720 2017-20 2 20172 2017-2 2017-

https://doi.org/10.1590/0074-02760220259

Preoperative risk factors associated with anastomotic leakage after colectomy for colorectal cancer: a systematic review and meta-analysis
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DIAS, VINÍCIUS EVANGELISTA

; CASTRO, PEDRO ALVES SOARES VAZ DE

; PADILHA, HOMERO TERRA

; PILLAR, LARA VICENTE

; GODINHO, LAURA BOTELHO RAMOS

; TINOCO, AUGUSTO CLAUDIO DE ALMEIDA

; AMIL, RODRIGO DA COSTA

; SOARES, ALEIDA NAZARETH

; CRUZ, GERALDO MAGELA GOMES DA

; BEZERRA, JULIANA MARIA TRINDADE

; SILVA, THAIS ALMEIDA MARQUES DA

Revista do Colégio Brasileiro de Cirurgiões

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2022, Volume 49 elocation e20223363

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO Objetivo: fístula anastomótica (FA) após colectomia para câncer colorretal (CCR) é complicação grave. Esta revisão sistemática e meta-análise avaliou os fatores de risco pré-operatórios para FA em pacientes submetidos à colectomia. Métodos: a pesquisa bibliográfica abrangeu 15 anos e 9 meses (1 de janeiro de 2005 - 19 de outubro de 2020), sendo utilizadas as plataformas PubMed, Cochrane Library, Scopus, Biblioteca Virtual em Saúde, Europe PMC e Web of Science. O critério de inclusão foram estudos transversais, coorte e caso-controle em fatores de risco pré-operatórios para FA (desfecho). A escala Newcastle-Ottawa foi usada para avaliação de viés dos estudos. A metanálise envolveu o cálculo dos efeitos de tratamento para cada estudo individualmente incluindo odds ratio (OR), risco relativo (RR) e intervalo de confiança de 95% (IC95%) com construção de modelo de efeitos aleatórios, para avaliar o impacto de cada variável (p<0,05). Resultados: foram selecionados 39 estudos transversais, 21 coortes e quatro casos-controle. A metanálise identificou 14 fatores de risco para FA em pacientes com CCR após colectomia, que são sexo masculino (RR=1,56; IC 95%=1,40-1,75), tabagismo (RR=1,48; IC 95%=1,30-1,69), alcoolismo (RR=1,35; IC 95%=1,21-1,52), diabetes mellitus (RR=1,97; IC 95%=1,44-2,70), doenças pulmonares (RR=2,14; IC 95%=1,21-3,78), doença pulmonar obstrutiva crônica (RR=1,10; IC 95%=1,04-1,16), doença coronariana (RR=1,61; IC 95%=1,07-2,41), doença renal crônica (RR=1,34; IC 95%=1,22-1,47), altas notas na escala ASA (RR=1,70; IC 95%=1,37-2,09), cirurgia abdominal prévia (RR=1,30; IC 95%=1,04-1,64), cirurgia de emergência (RR=1,61; IC 95%=1,26-2,07), quimioterapia neoadjuvante (RR=2,16; IC 95%=1,17-4,02), radioterapia (RR=2,36; IC 95%=1,33-4,19) e quimiorradioterapia (RR=1,58; IC 95%=1,06-2,35). Conclusões: importantes fatores de risco pré-operatórios para FA colorretais em pacientes com CCR foram identificados com base nas melhores pesquisas baseadas em evidências e esse conhecimento deve influenciar decisões relacionadas ao tratamento.

ABSTRACT Introduction: anastomotic leak (AL) after colectomy for colorectal cancer (CRC) is a life-threatening complication. This systematic review and meta-analysis aimed to evaluate the preoperative risk factors for AL in patients submitted to colectomy. Methods: the bibliographic search covered 15 years and 9 months, from 1st January 2005 to 19th October 2020 and was performed using PubMed, Cochrane Library, Scopus, Biblioteca Virtual em Saúde, Europe PMC and Web of Science databases. The inclusion criteria were cross-sectional, cohort and case-control studies on preoperative risk factors for AL (outcome). The Newcastle-Ottawa scale was used for bias assessment within studies. Meta-analysis involved the calculation of treatment effects for each individual study including odds ratio (OR), relative risk (RR) and 95% confidence intervals (95% CI) with construction of a random-effects model to evaluate the impact of each variable on the outcome. Statistical significance was set at p<0.05. Results: cross-sectional studies were represented by 39 articles, cohort studies by 21 articles and case-control by 4 articles. Meta-analysis identified 14 main risk factors for AL in CRC patients after colectomy, namely male sex (RR=1.56; 95% CI=1.40-1.75), smoking (RR=1.48; 95% CI=1.30-1.69), alcohol consumption (RR=1.35; 95% CI=1.21-1.52), diabetes mellitus (RR=1.97; 95% CI=1.44-2.70), lung diseases (RR=2.14; 95% CI=1.21-3.78), chronic obstructive pulmonary disease (RR=1.10; 95% IC=1.04-1.16), coronary artery disease (RR=1.61; 95% CI=1.07-2.41), chronic kidney disease (RR=1.34; 95% CI=1.22-1.47), high ASA grades (RR=1.70; 95% CI=1.37-2.09), previous abdominal surgery (RR=1.30; 95% CI=1.04-1.64), CRC-related emergency surgery (RR=1.61; 95% CI=1.26-2.07), neoadjuvant chemotherapy (RR=2.16; 95% CI=1.17-4.02), radiotherapy (RR=2.36; 95% CI=1.33-4.19) and chemoradiotherapy (RR=1.58; 95% CI=1.06-2.35). Conclusions: important preoperative risk factors for colorectal AL in CRC patients have been identified based on best evidence-based research, and such knowledge should influence decisions regarding treatment.

https://doi.org/10.1590/0100-6991e-20223363-en

Glycerin supplementation strategies for three or seven days affects oxidative stress, follicle dynamics and ovulatory response in Morada Nova sheep
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Andrade, Mariza Araújo Morais de Moura

; Alves, Juliana Paula Martins

; Galvão, Iolly Tábata Oliveira Marques

; Cavalcanti, Camila Muniz

; Silva, Maria Raquel Lopes

; Conde, Alfredo José Herrera

; Bezerra, Alessandra Façanha

; Fernandes, César Carneiro Linhares

; Teixeira, Dárcio Italo Alves

; Rondina, Davide

Animal Reproduction

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2022, Volume 19 N. 2 elocation e20200025

Abstract: En | Text: En | PDF: En

Abstract This study examined the effect of glycerin supply strategies in different short-term protocols on follicular dynamics and ovulatory rate in Morada Nova sheep. Eighteen Morada Nova ewes with body condition > 2.9 had their estrus and follicular waves synchronized using three injections of prostaglandin analogue at seven-day intervals. All animals received the same diet during 21 days, which consisted of a total mixed ration (TMR) based on chopped elephant grass and concentrate twice daily. In thecontrol group (n=9), ewes were fed the TMR diet. In the other four groups, ewes received 150 mL of glycerol daily, supplied as an oral drench or mixed in the TMR during three or seven days prior to the application of thethird PGF2 alfa analogue. These groups were named as follows: Drench3d (n=10), Drench7d (n=8), TMR3d (n=9) and TMR7d (n=9). Follicle dynamics were monitored by ultrasonography, and plasma glucose and glutathione peroxidase levels were measured at the third prostaglandin administration. Six days after the final PGF2 alfa analogue dose, ovulatory rate was measured by laparoscopy. Glucose was higher (P< 0.001) in the glycerin-treated groups than in control group (83.7 ± 1.7 vs. 68.4 ± 4.5 mg. dL-1; P < 0.001). Ewes in the TMR3d, Drench7d and TMR7d groups had a greater (P < 0.001) number of large follicles (≥ 3 < 5 mm), and the presence of follicles larger than 5 mm was observed. In the same groups, at the third PGF2 alfa analogue dose, a greater (P < 0.001) number of growing follicles (> 3 mm) and a larger size of the largest follicle (P < 0.001) were also recorded. Ovulation rate was 30% higher in the groups that received glycerin for seven days (1.6 ± 0.1 53 vs. 1.1 ± 0.1; P < 0.05), and they also exhibited a 38% reduction in glutathione peroxidase. Thus,the use of glycerin in Morada Nova sheep as a source of energy in short-term supplementation for increase ovulation rate is an efficient strategy when provided for seven days, either orally or in the feed.

https://doi.org/10.1590/1984-3143-ar2020-0025

Regional high-grade metamorphic peak imprint in zircons from the mafic-ultramafic Jacurici Complex, São Francisco Craton, Brazil
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Dias, João Rodrigo Vargas Pilla

; Marques, Juliana Charão

; Bertolini, Gabriel

; Frantz, José Carlos

; Friedrich, Betina

; Paim, Jhenifer Caroline da Silva

; Silveira, Carlos José Sobrinho da

; Queiroz, Waldemir José Alves

Brazilian Journal of Geology

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2022, Volume 52 N. 1 elocation e20210007

Abstract: En | Text: En | PDF: En

Abstract The Cr-rich Jacurici Complex is located at the limit between the Serrinha Block and the Salvador-Curaçá Belt, Northeastern São Francisco Craton. In order to better understand the age span of magmatism and tectonism, we have studied a basement high-grade charnockite orthogneiss, a metanorite of the Complex and a crosscuting undeformed quartz-feldspar pegmatite. For the orthogneiss, SHRIMP U-Pb ages of zircon cores and overgrowth rims yielded, 2966 ± 9 Ma for crystallization and 2091 ± 10 Ma for metamorphism, respectively. Zircons from the metanorite show internal textures consistent with high grade metamorphism. The U-Pb LA-ICP-MS age determination provided a 2099 ± 6 Ma metamorphic age for the Jacurici Complex, indicating it was already emplaced during amalgamation around 2.1 Ga. Zircons from the undeformed quartz-feldspar pegmatite provided a LA-ICP-MS U-Pb age of 2083 ± 11Ma, marking the end of the N-S transcurrence. The magmatic Jacurici Complex age remains undefined, but is equal to, or older than, 2.1 Ga. Therefore, possible correlations with other mafic-ultramafic intrusions, such as the Cu-rich Caraíba Complex, should be reassessed. Our results indicate that the N-S transcurrence ended earlier in the Jacurici Complex region than in the northwestern area of the Salvador-Curaçá belt.

https://doi.org/10.1590/2317-4889202120210007

Insegurança alimentar em agricultores: violação do direito humano à alimentação adequada
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Costa, Lucas Alves da

; Marques, Amanda de Andrade

; Sampaio, Juliana Ribeiro Francelino

; Santana, Willma José de

; Rocha, Elida Mara Braga

Cadernos Saúde Coletiva

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mar 2021, Volume 29 N. 1 Pages 122 - 132

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Resumo Introdução No Brasil, vislumbraram-se avanços significativos nas políticas de alimentação e nutrição. Apesar disso, o país apresenta milhões de pessoas em condições de Insegurança Alimentar (IA), com reconhecida dificuldade de acesso à alimentação adequada. Objetivo Esta pesquisa teve como objetivo investigar a associação entre insegurança alimentar com o perfil antropométrico e socioeconômico de agricultores dos municípios da região centro-sul do Ceará. Método Trata-se de uma pesquisa transversal que utilizou um questionário socioeconômico e a Escala Brasileira de Insegurança Alimentar (EBIA) para avaliar a situação de IA e o Índice de Massa Corporal (IMC) para avaliação do estado nutricional. Os testes de Mann-Whitney e qui-quadrado foram utilizados, considerando significativo p < 0,05. Resultados Participaram desta pesquisa 86 agricultores, destes 39,5% em condições de IA e 41% com sobrepeso. Foi identificada associação entre a situação de IA em famílias com o maior número de indivíduos (p < 0,05), a presença de menores de 18 anos (p < 0,05) e menor renda per capita (p = 0,001). Conclusão Podemos concluir que a IA esteve associada fortemente à aglomeração domiciliar e renda entre famílias de agricultores. Assim, as políticas públicas voltadas à saúde dos agricultores devem considerar a vulnerabilidade das famílias de baixa renda e com crianças e jovens na sua composição, possibilitando acesso à alimentação adequada.

Abstract Background In Brazil, it has been envisioned significant advances in food and nutrition policies. Despite this, the country has millions of people under conditions of Food Insecurity (FI), with recognized difficulties of access to adequate food. Objective This research aimed to investigate the association between FI with anthropometric and socioeconomic statusof farmers of the municipalities in the Middle-South region of Ceará. Method It is a cross-sectional study that used a socioeconomic questionnaire and the Brazilian Food Insecurity Scale to assess the situation of FI and the Body Mass Index (BMI) for assessing the nutritional status. The tests of Mann-Whitney and Chi-square were used, considering a significant p-value (p<0.05). Results 86 farmers participated in this research, including 39.5% of those who wereFI and 41% overweight. The association was identified between the situation of FI in families with the greatest number of individuals (p<0.05), the presence of individuals aged under18 years (p<0.05) and lower per capita income (p=0.001). Conclusion It could be conclude that the FI was strongly associated to household crowding and income among farmers’ families Thus, the public policies directed to the farmers’ health should consider the vulnerability of low-income families and families with children as well as young people in their composition, enabling access to adequate food.

https://doi.org/10.1590/1414-462x202129010471 355 downloads

10.

How to drive phloem gene expression? A case study with preferentially expressed citrus gene promoters
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Bezerra, Yane Caroline dos Anjos

; Marques, João Paulo Rodrigues

; Stipp, Liliane Cristina Liborio

; Attílio, Lísia Borges

; Freitas-Astúa, Juliana

; Mourão Filho, Francisco de Assis Alves

Revista Brasileira de Fruticultura

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2021, Volume 43 N. 4 elocation e-005

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Resumo Novas abordagens para o desenvolvimento de organismos geneticamente modificados, resistentes a doenças, incluem alvos específicos de expressão gênica, visando a aumentar as chances de controle do patógeno. A expressão gênica, induzida por promotores de genes de Citrus sinensis, derivados do floema, poderia ser avaliada e comparada com aquela induzida por um promotor constitutivo no mesmo tecido, levando à produção de laranjas-doces transgênicas, potencialmente mais resistentes a doenças causadas por bactérias de floema. Plantas de citrange ‘Carrizo’ [Poncirus trifoliata (L.) Raf. x Citrus sinensis (L.) Osbeck] foram transformadas, via Agrobacterium tumefaciens, com o vetor binário pCAMBIA2301 contendo o gene uidA (ß-glucuronidase), dirigido pelo promotor constitutivo CaMV35S (CaMV35S :: uidA) ou pelos promotores de citros CsPP2.B1 (CsPP2 .B1 :: uidA) ou CsVTE2 (CsVTE2 :: uidA). Brotos regenerados in vitro foram enxertados em limão ‘Cravo’ (C. limonia Osbeck). A transformação genética foi confirmada por análises de Southern blot. A expressão do gene uidA foi avaliada por RT-qPCR, e a histolocalização do gene, dirigida por esses três promotores, foi acessada por seções de ramos tratadas com X-GLUC. A expressão do gene uidA, exibida por promotores específicos de tecido, foi, em geral, menor do que a do promotor constitutivo CaMV35; no entanto, as construções dirigidas por promotores específicos de tecido podem levar à expressão em tecidos restritos. Os promotores CsPP2.B1 e CsVTE2 podem ser considerados adequados para utilização em construções gênicas, visando à resistência a doenças.

Abstract New approaches for developing disease-resistant genetically modified organisms have included specific targets for gene expression to enhance the chances for pathogen control. Gene expression driven by phloem-derived Citrus sinensis gene promoters could be evaluated and compared with the expression induced by a strong constitutive promoter in the same tissue, leading to the production of transgenic sweet oranges potentially more resistant to diseases caused by phloem-limited bacteria. ‘Carrizo’ citrange [ (Poncirus trifoliataL.) Raf. x Citrus sinensis (L.) Osbeck] was transformed, via Agrobacterium tumefaciens, with the binary vector pCAMBIA2301 bearing the uidA gene (ß-glucuronidase) driven by the CaMV35S constitutive promoter (CaMV35S::uidA) or by the CsPP2.B1 (CsPP2.B1::uidA) or by the CsVTE2 (CsVTE2::uidA) citrus promoters. In vitro regenerated shoots were grafted onto ‘Rangpur’ lime (C. limonia Osbeck). The genetic transformation was confirmed by Southern blot analyses. uidA gene expression was evaluated by RT-qPCR, and gene histolocalization controlled by these three promoters was accessed by X-GLUC treated stem sections. uidA gene expression exhibited by tissue-specific promoters was overall lower than from the constitutive promoter CaMV35; however, constructs driven by tissue-specific promoters may lead to expression in restricted tissues. CsPP2.B1 and CsVTE2 promoters can be considered adequate for the utilization in gene constructs aiming disease resistance.

https://doi.org/10.1590/0100-29452021005 143 downloads

11.

Experiences of family members of children diagnosed with autism spectrum disorder
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Magalhães, Juliana Macêdo

; Rodrigues, Thalia Alves

; Neta, Marly Marques Rêgo

; Damasceno, Carolinne Kilcia Carvalho Sena

; Sousa, Kayo Henrique Jardel Feitosa

; Arisawa, Emília Ângela Lo Schiavo

Revista Gaúcha de Enfermagem

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2021, Volume 42 elocation e20200437

Abstract: Pt En Es | Text: Pt En Es | PDF: Pt | PDF: En | PDF: Es

RESUMO Objetivo: Descrever, na percepção das mães, as experiências vivenciadas por famílias no cuidado de crianças com Transtorno do Espectro Autista. Método: Estudo qualitativo, realizado com 20 mães de crianças diagnosticadas com transtorno autístico, acompanhadas por uma instituição em Teresina, Piauí. Entrevistas semiestruturadas foram realizadas entre fevereiro e março de 2019, e submetidas à análise de conteúdo. Resultados: Foram identificadas cinco ideias centrais relacionadas aos estágios vividos pelos familiares após o diagnóstico, desde a negação até a aceitação. Familiares e cuidadores vivenciam sentimentos de tristeza e luto pela descoberta da impossibilidade de cura da síndrome, revelando a necessidade de cuidado para com essa família. A busca por ajuda e as adaptações da rotina são vivências constantes. Conclusão: Cuidar de crianças com transtorno autístico envolve aprendizados que vão dos aspectos estruturais aos emocionais, como lidar com as limitações e impossibilidade de cura, apontando para a necessidade de um cuidado familiar.

ABSTRACT Objective: To describe, in the mothers’ perception, the experiences lived by families in the care of children with autism spectrum disorder. Method: Qualitative study, carried out with 20 mothers of children diagnosed with autistic disorder accompanied by an institution in Teresina-Piauí, Brazil. Semi-structured interviews were conducted between February and March 2019 and subjected to content analysis. Results: Five central ideas related to the stages experienced by family members after the diagnosis were identified, ranging from denial to acceptance. Family members and caregivers experience feelings of sadness and mourning for the discovery of the impossibility of curing the syndrome, revealing the need for care for this family. The search for help and adaptations of the routine are constant experiences. Conclusion: Caring for children who live with autistic disorder involves learning ranging from structural to emotional aspects, such as dealing with limitations and impossibility of cure, pointing out to the need for family care.

RESUMEN Objetivo: Describir, en la percepción de las madres, las experiencias vividas por las familias en el cuidado de niños con trastorno del espectro autista. Método: Estudio cualitativo, realizado con 20 madres de niños diagnosticados de trastorno autista acompañados de una institución en Teresina-Piauí, Brasil. Las entrevistas semiestructuradas se realizaron entre febrero y marzo de 2019 y se sometieron a análisis de contenido. Resultados: Se identificaron cinco ideas centrales relacionadas con las etapas vividas por los familiares luego del diagnóstico, que van desde la negación hasta la aceptación. Los familiares y cuidadores experimentan sentimientos de tristeza y duelo por el descubrimiento de la imposibilidad de curar el síndrome, revelando la necesidad de cuidar a esta familia. La búsqueda de ayuda y adaptaciones de la rutina son experiencias constantes. Conclusión: Cuidar a los niños que viven con trastorno autista implica aprender que van desde los aspectos estructurales hasta los emocionales, cómo afrontar las limitaciones y la imposibilidad de cura, apuntando a la necesidad del cuidado familiar.

https://doi.org/10.1590/1983-1447.2021.20200437

12.

COVID-19: The question of genetic diversity and therapeutic intervention approaches
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Figueiredo, David Livingstone Alves

; Ximenez, João Paulo Bianchi

; Seiva, Fábio Rodrigues Ferreira

; Panis, Carolina

; Bezerra, Rafael dos Santos

; Ferrasa, Adriano

; Cecchini, Alessandra Lourenço

; Medeiros, Alexandra Ivo de

; Almeida, Ana Marisa Fusco

; Ramão, Anelisa

; Boldt, Angelica Beate Winter

; Moya, Carla Fredrichsen

; Chin, Chung Man

; Paula, Daniel de

; Rech, Daniel

; Gradia, Daniela Fiori

; Malheiros, Danielle

; Venturini, Danielle

; Tavares, Eliandro Reis

; Carraro, Emerson

; Ribeiro, Enilze Maria de Souza Fonseca

; Pereira, Evani Marques

; Tuon, Felipe Francisco

; Follador, Franciele Aní Caovilla

; Fernandes, Glaura Scantamburlo Alves

; Volpato, Hélito

; Cólus, Ilce Mara de Syllos

; Oliveira, Jaqueline Carvalho de

; Rodrigues, Jean Henrique da Silva

; Santos, Jean Leandro dos

; Visentainer, Jeane Eliete Laguila

; Brandi, Juliana Cristina

; Serpeloni, Juliana Mara

; Bonini, Juliana Sartori

; Oliveira, Karen Brajão de

; Fiorentin, Karine

; Lucio, Léia Carolina

; Faccin-Galhardi, Ligia Carla

; Ferreto, Lirane Elize Defante

; Lioni, Lucy Megumi Yamauchi

; Consolaro, Marcia Edilaine Lopes

; Vicari, Marcelo Ricardo

; Arbex, Marcos Abdo

; Pileggi, Marcos

; Watanabe, Maria Angelica Ehara

; Costa, Maria Antônia Ramos

; Giannini, Maria José S. Mendes

; Amarante, Marla Karine

; Khalil, Najeh Maissar

; Lima Neto, Quirino Alves de

; Herai, Roberto H.

; Guembarovski, Roberta Losi

; Shinsato, Rogério N.

; Mainardes, Rubiana Mara

; Giuliatti, Silvana

; Yamada-Ogatta, Sueli Fumie

; Gerber, Viviane Knuppel de Quadros

; Pavanelli, Wander Rogério

; Silva, Weber Claudio da

; Petzl-Erler, Maria Luiza

; Valente, Valeria

; Soares, Christiane Pienna

; Cavalli, Luciane Regina

; Silva Jr, Wilson Araujo

Genetics and Molecular Biology

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2021, Volume 44 N. 1 elocation e20200452

Abstract: En | Text: En | PDF: En

Abstract Coronavirus disease 2019 (COVID-19), caused by the Severe Acute Respiratory Syndrome Coronavirus type 2 (SARS-CoV-2), is the largest pandemic in modern history with very high infection rates and considerable mortality. The disease, which emerged in China’s Wuhan province, had its first reported case on December 29, 2019, and spread rapidly worldwide. On March 11, 2020, the World Health Organization (WHO) declared the COVID-19 outbreak a pandemic and global health emergency. Since the outbreak, efforts to develop COVID-19 vaccines, engineer new drugs, and evaluate existing ones for drug repurposing have been intensively undertaken to find ways to control this pandemic. COVID-19 therapeutic strategies aim to impair molecular pathways involved in the virus entrance and replication or interfere in the patients’ overreaction and immunopathology. Moreover, nanotechnology could be an approach to boost the activity of new drugs. Several COVID-19 vaccine candidates have received emergency-use or full authorization in one or more countries, and others are being developed and tested. This review assesses the different strategies currently proposed to control COVID-19 and the issues or limitations imposed on some approaches by the human and viral genetic variability.

https://doi.org/10.1590/1678-4685-gmb-2020-0452

13.

Disseminated tuberculosis associated with reactive arthritis of Poncet in an immunocompetent patient,
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Calado, Juliana de Oliveira Alves

; Miola, Anna Carolina

; Silvares, Maria Regina Cavariani

; Marques, Silvio Alencar

Anais Brasileiros de Dermatologia

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jun 2020, Volume 95 N. 3 Pages 343 - 346

Abstract: En | Text: En | PDF: En

Abstract Cutaneous tuberculosis is a rare extrapulmonary manifestation of tuberculosis which, like disseminated tuberculosis, commonly occurs in immunocompromised patients. Poncet reactive arthritis is a seronegative arthritis affecting patients with extrapulmonary tuberculosis, which is uncommon even in endemic countries. We report a previously healthy 23-year-old male patient with watery diarrhea associated with erythematous ulcers on the lower limbs and oligoarthritis of the hands. Histopathological examination of the skin showed epithelioid granulomatous process with palisade granulomas and central caseous necrosis. AFB screening by Ziehl-Neelsen staining showed intact bacilli, the culture was positive for Mycobacterium tuberculosis, and colonoscopy revealed multiple shallow ulcers. Disseminated tuberculosis associated with reactive Poncet arthritis was diagnosed, with an improvement of the clinical and skin condition after appropriate treatment.

https://doi.org/10.1016/j.abd.2019.08.031 280 downloads

14.

[SciELO Preprints] - Implicações Cardiovasculares em Pacientes Infectados com Covid-19 e a Importância do Isolamento Social para Reduzir a Disseminação da Doença
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Costa, Juliana Alves Silveira, Juliana de Almeida Santos, Sara Cristine Marques dos Nogueira, Patrícia Pereira

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Publication date: 2020-05-28

Abstract: Pt

Os sintomas respiratórios, principalmente o desenvolvimento de quadros de síndrome do desconforto respiratório agudo grave, dominam a discussão e as preocupações iniciais da população e dos profissionais de saúde. Entretanto, o sistema cardiovascular é bastante afetado por essas condições e, muitas vezes, é o responsável por complicações e mortalidade desses pacientes. Com o objetivo de mostrar as implicações cardiovasculares em pacientes infectados pela COVID-19 e a importância do isolamento social como alternativa de frear a disseminação da doença, foi realizada uma revisão da literatura com base em 37 artigos, nos idiomas inglês, português e espanhol, disponíveis na plataforma Scielo e PubMed. Os resultados mostraram que complicações cardíacas associadas à infecção pela COVID-19 são semelhantes às produzidas por: síndrome respiratória aguda grave (SARS), síndrome respiratória do Médio Oriente (MERS) e influenza. Contudo, a COVID-19 apresenta uma contaminação muito maior e mais rápida e, ao contrário da gripe por influenza, ainda não existe vacina disponível ou tratamento. Diante disso, o isolamento social passa a ser uma ferramenta que pode reduzir e achatar a curva de casos incidentes e assim preservar as pessoas que se enquadrem no grupo de risco, diminuindo as chances de quadros graves da doença, possíveis óbitos e o colapso no sistema de saúde do país.

15.

[SciELO Preprints] - Cardiovascular Implications in Patients Infected with Covid-19 and the Importance of Social Isolation to Reduce Dissemination of the Disease
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Costa, Juliana Alves Silveira, Juliana de Almeida Santos, Sara Cristine Marques dos Nogueira, Patrícia Pereira

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Publication date: 2020-05-28

Abstract: En

Respiratory symptoms, especially the development of severe acute respiratory distress syndrome, dominate the discussion and initial concerns of the population and health professionals. However, the cardiovascular system is greatly affected by these conditions and is often responsible for complications and mortality of these patients. In order to show the cardiovascular implications in patients infected with COVID-19 and the importance of social isolation as an alternative to curb the spread of the disease, a literature review was carried out based on 37 articles, in English, Portuguese and Spanish, available on Scielo and PubMed. The findings showed that cardiac complications associated with COVID-19 infection are similar to those produced by: severe acute respiratory syndrome (SARS), Middle East respiratory syndrome (MERS) and influenza. However, COVID-19 has a much greater and faster contamination and, unlike influenza, there is no vaccine or treatment available yet. In view of this, social isolation becomes a tool that can reduce and flatten the curve of cases and thus protect the people at higher risk, decreasing the chances of serious conditions related to the disease, potential deaths and the collapse of the country’s health system.

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