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Sistema de transferencia de datos biomédicos con protocolos de comunicación de bajo consumo
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Becerra Tapia, Víctor

; Téllez Victoria, Victoria

; Ramos Medina, José Mariano

; Peñaloza Mendoza, Guillermo Rey

; Castro Zenil, Mario Salvador

Revista de ciencias tecnológicas

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dec 2023, Volume 6 N. 4 elocation e284

Abstract: En Es | Text: En Es | PDF: En | PDF: Es

Abstract. - In the field of medical care, hospitals face numerous challenges in effectively managing biomedical data. This can lead to a decrease in the efficiency of care, as not all institutions have efficient methods for managing such data. In addition to personal data, physiological constants, such as heart rate and oxygen levels, need to be constantly monitored in order to detect any changes. However, obtaining this data from different instruments and ensuring its constant recording can be problematic. To address these challenges, a system based on the Internet of Things (IoT) has been developed. This system utilizes sensors connected to ESP32 cards, which are in constant communication, to obtain physiological constants and other relevant data. A prototype has been designed, which includes sensors placed on the wrist to measure three physiological constants. The MAX30102 pulse sensor is used to measure blood oxygenation and heart rate. This sensor can be placed on the fingers, lobe, or wrist to obtain accurate readings. Additionally, the MLX90614 sensor is used for temperature acquisition. All the data collected by these sensors is managed by an ESP32 card, which acquires the information and sends it for further use. They employ communication protocols that enable the simultaneous reading of multiple sensors for the parallel monitoring of more than one patient, a capability not addressed in current prehospital care systems. To ensure constant monitoring of physiological constants, a master-slave configuration is utilized. Each slave module collects information from individual patients and sends it to a master card. The data is encrypted during transmission. These devices can be used in various healthcare settings, including prehospital care, and can be carried by the patients themselves. The collected data is then transmitted to a central system using the MQTT protocol. A master ESP32, connected to a Raspberry Pi 4, acts as the main console, where the data is centralized. Once the data is in the MQTT broker, it can be accessed and analyzed from various devices for traceability purposes. Real-time data recording is achieved by utilizing Google services, specifically Firebase, which stores the data in a database.

Resumen. - En la atención médica dentro de un hospital son múltiples los eventos que se presentan a diario, en donde el manejo de datos biomédicos es compartido en todas las áreas, lo que se torna complicado y no en todas las instituciones se tienen métodos adecuados para la gestión de estos datos, lo que provoca una disminución en la eficiencia de la atención. Además de los datos personales, las constantes fisiológicas son datos que deben llevar un monitoreo constante de lo contrario es un problema al tener signos cambiantes, estos datos se obtienen de diferentes instrumentos y se lleva el registro de una sola lectura sin ser constante. Debido a esto se desarrolla un sistema basado en el internet de las cosas, en el cual la obtención de las constantes fisiológicas y otros datos se realiza a través de sensores conectados a tarjetas ESP32 que están en constante comunicación. Para la adquisición de datos, se diseña un prototipo el cual se coloca en la muñeca con los sensores incluidos para la toma de 3 constantes fisiológicas. Se emplea el sensor de pulso MAX30102 que permite obtener la oxigenación de la sangre y la frecuencia cardiaca, para obtener la lectura del pulso y/o ritmo cardiaco se coloca el dispositivo en los dedos, lóbulo o muñeca. Además, se cuenta con el sensor MLX90614 para la adquisición de la temperatura. El manejo de datos se realiza por medio de una tarjeta ESP32 que adquiere la información de los sensores y la envía para su utilización. Se utilizan protocolos de comunicación que permite la lectura de múltiples sensores para la lectura paralela de más de un paciente, esto no se ha tratado en los sistemas actuales de atención prehospitalaria. El constante monitoreo independiente de las constantes fisiológicas se comunica a una central por medio de una configuración esclavos maestro, en donde cada esclavo obtiene la información de cada módulo en el paciente y es enviada a una tarjeta maestra, todos los datos son encriptados en cada envió, estos dispositivos pueden ser utilizados en atención prehospitalaria y dentro de los hospitales portados por el propio paciente. La concentración de los datos es recabada en una ESP32 maestra la cual se conecta de forma serial a la consola principal que es una Raspberry Pi 4, a través del protocolo MQTT, una vez que se tienen los datos en MQTT broker se pueden visualizar en diferentes dispositivos para la trazabilidad de estos datos. Los datos son registrados en una base de datos en tiempo real usando los servicios de Google en Firebase.

https://doi.org/10.37636/recit.v6n4e284

Análisis epidemiológico del brote de sarampión 2020 en la zona norte de la Ciudad de México
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Gómez-Santana, Mariano

; Huerta-Manzano, Mario A.

; Hernández-Rodríguez, Paola

; Vega-Mendoza, Santa

; Camarillo-Nava, Víctor M.

Revista mexicana de medicina familiar

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dec 2022, Volume 9 N. 4 Pages 101 - 108

Abstract: Es En | Text: Es En | PDF: Es | PDF: En

RESUMEN Introducción: El sarampión era una enfermedad común en la infancia, que con elevadas coberturas vacunales, se han producido brotes en distintas regiones por movilidad geográfica. Objetivo: Determinar la prevalencia epidemiológica y clínica del brote de sarampión en el periodo de enero a junio del 2020 en la Ciudad de México/Delegación Norte del IMSS. Material y métodos: Estudio retrospectivo, descriptivo de 124 datos de casos notificados en OOAD Norte D.F. IMSS, enero-junio 2020, bajo consentimiento informado, obteniendo datos sociodemográficos, semana notificación, manifestaciones clínicas, antecedente inmunización, casos sospechososo confirmados mediante prueba de laboratorio, utilizando estadística descriptiva con un, intervalo de confianza de 95%. Resultados: La prevalencia de sarampión fue del 0.00006063%, la mediana de edad fue de 7 años (intervalo intercuartílico: 26 años. Grupo etario más afectado de 1-4 años (27.4%); hombres en 52.4% y 62.9% residentes de la Alcaldía Gustavo A. Madero. La semana con más casos fue la 13 (22 al 28 de marzo) con 19.4%. Antecedente de inmunización un 36.3%. El diagnóstico en el 19.4% de los casos fue confirmatorio por laboratorio, y el 80.6% se mantuvo como sospechoso por presentar manifestaciones clínicas. Conclusiones: La prevalencia del brote de sarampión en la Delegación Norte del IMSS, entre enero y junio de 2020 fue de 0.00006063% (6.063 por 100,000 habitantes) siendo diagnosticados principalmente por sus características clínicas.

ABSTRACT Introduction: Measles was a common disease that, with high vaccination coverage, has caused outbreaks in different regions due to geographic mobility. Objective: To determine the epidemiological and clinical prevalence of the measles outbreak from January to June 2020 in Mexico City/IMSS North Delegation. Materials and methods: Retrospective, descriptive study in 124 reported cases of measles in the OOAD Norte D.F. IMSS; January-June 2020, under informed consent, obtaining sociodemographic data, week of notification, clinical manifestations, immunization history, suspected case or confirmed by confirmatory serum test and pharyngeal exudate. Using descriptive statistics with a confidence interval of 95%. Results: Prevalence of measles was 0.00006063%, the median age was 7 years (IQR = 26 years). The most affected age group was 1-4 years (27.4%). 52.4% of the sample were men. 62.9 % were residents of the Gustavo A. Madero Mayor’s Delegation The week with the most cases was 13 (March 22 to 28) with 19.4% History of immunization 36.3% and 63.7% without immunization Diagnosis in 19.4% of the cases were confirmed by laboratory and 80.6% remained suspicious for presenting clinical manifestations of measles. Conclusions: The epidemiological and clinical prevalence of the measles outbreak from January to June 2020 in Mexico City/Delegación Norte IMSS was determined, being 0.00006063 (a rate of 6,063 cases per 100 thousand inhabitants), being mainly diagnosed by clinical characteristics.

https://doi.org/10.24875/rmf.22000034

Taller de concordancia en la evaluación de imágenes capilaroscópicas
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Izaguirre, Pía

; Romanini, Félix Enrique

; Mamani, Marta

; Montoya, Sandra Fabiana

; Toibar, Javier

; Catalán Pellet, Santiago

; Sirera, Ana Irene

; Dueñas, Lelly

; Cucchiaro, Natalia

; Cousseau, Mario

; Orellana González, Carlos Alberto

; Yucra, Demelza

; Tralice, Elda Rossella

; Raschia, Alan Juan

; Molina, María Josefina

; Lencia, María Verónica

; Espasa, Gabriela Vanesa

; Linarez Martínez, Miguel Alex

; Castro Coello, Vanesa Viviana

; García, Aldo

; Velloso, María Sofía

; González, Paula Alejandra

; German, Noelia Fernanda

; Conti, Silvana

; Savy, Florencia

; Morbiducci, Julieta Silvana

; Rivero, Mariano

; Saavedra, Leandro José

; Sabelli, Mirtha Rosa

; Rebak, Jonathan Eliseo

; Rojas Tessel, Romina

; Sorrentino, Laura

; Gervasoni, Viviana Lorena

; Marcantoni, María Beatriz

; Gervilla de Cafrune, Ana Lía

; Got, Julio

; Sánchez, Gabriela

; Castorino, Gerardo

; García, Lucila

; Sansinanea, Pierina

; Tamborenea, María Natalia

Revista argentina de reumatología

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oct 2022, Volume 33 N. 4 Pages 223 - 227

Abstract: Es En | Text: Es En | PDF: Es | PDF: En

Resumen Introducción: la capilaroscopia es un método no invasivo que permite observar la microvasculatura en el área periungueal. Los resultados informados pueden ser altamente variables entre distintos observadores. A lo largo del tiempo surgieron métodos cuantitativos y semicuantitativos para mejorar la reproducibilidad. Objetivos: conocer el nivel de acuerdo intra e interobservador al informar los diferentes patrones capilaroscópicos en individuos con diferente nivel de entrenamiento. Materiales y métodos: estudio de corte transversal. Participaron médicos reumatólogos especialistas y en formación que habían realizado previamente un curso virtual de capacitación en capilaroscopia. Recibieron 40 imágenes capilaroscópicas proyectadas en una presentación de PowerPoint y debían responder a través de un cuestionario digital. Se evaluó la concordancia de respuestas intra e interobservador. Resultados: se encontró un alto nivel de concordancia global con un kappa 0,66 IC 95% (0,63-0,70) p<0,0000. También en otros grupos como reumatólogos en formación: kappa 0,65 IC 95% (0,60-0,71) p=0,0000, y médicos reumatólogos: kappa 0,67 IC 95% (0,62-0,72) p=0,0000. Conclusiones: el nivel de concordancia encontrado fue globalmente alto, independientemente del nivel de entrenamiento de los profesionales, y de ser o no reumatólogo. La concordancia fue superior cuando se comparó a quienes tenían más de 4 años de experiencia en la realización de videocapilaroscopia.

Abstract Introduction: videoapillaroscopy is a non-invasive method that allows the observation of the microvasculature in the periungual area. Reported results can be highly variable between different observers. Over time, quantitative and semi-quantitative methods emerged to improve reproducibility. Objetives: to know the level of intra and interobserver agreement when reporting the different capillaroscopic patterns in individuals with different levels of training. Materials and methods: cross section study. Specialist rheumatologists and those in training who had previously completed a virtual capillaroscopy training course participated. They received 40 capillaroscopic images projected in a PowerPoint presentation and had to issue their response through a digital questionnaire. Concordance of intra and interobserver responses was evaluated. Results: a high level of global agreement was found with a kappa 0.66 CI 95% (0.63-0.70) p<0.0000, also in other groups such as rheumatologists in training: kappa 0.65 CI 95% (0.60-0.71) p=0.0000, physicians rheumatologists: kappa 0.67 95% CI (0.62-0.72) p=0.0000. Conclusions: the level of agreement found was globally high, regardless of the level of training of the professionals, and whether or not they were a rheumatologist. Concordance was higher when compared to those who had more than 4 years of experience performing videocapillaroscopy.

https://doi.org/10.47196/rar.v33i4.709

Bioestructura de la Piel de la Aleta Caudal del Salmón del Atlántico, en Estado Smolt y sus Interacciones con el Estado Chalimus del Caligus Rogercresseyi
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Pellón, Mario

; Smok, Carolina

; Vásquez, Bélgica

; del Sol, Mariano

; Rojas, Mariana

International Journal of Morphology

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2022, Volume 40 N. 2 Pages 489 - 494

Abstract: En Es | Text: En Es | PDF: En | PDF: Es

SUMMARY: Caligus rogercresseyi is a copepod that represents one of the main challenges of the salmon farming industry in Chile, since it profusely affects the skin. It should be noted that fish in freshwater and estuaries are not affected, unlike salmon, which from post-smolt is highly parasitized when transferred to the sea. Multiple studies have been carried out on the life cycle of the parasite and chemical, physical and mechanical treatments have been developed to eliminate them. However, to date, the treatments have not been effective, which produces a permanent problem for the welfare of the animal. The purpose of this study was to recognize the biostructure of Atlantic salmon caudal fin skin at sites of interaction with chalimus. For this, 15 post-smolt infected with Caligus and 5 post-smolt controls, without Caligus, were used. The salmon were provided by Fundación Chile and the experience was carried out in its own experimental center. Once the euthanasia was carried out, by means of an overdose of the anesthetic benzocaine, samples of the caudal fins were obtained, which were fixed in 10 % formalin, included in paraplast to make 5 µm-thick sections and stained with Masson's Trichrome and PAS. The results indicated that the skin of the caudal fin of the affected post-smolt presented a greater height of the epidermis, few mucus-secreting cells and a solution of continuity in the epidermis. In addition, the basement membrane is discontinued and an increase in melanomacrophages occurs in the dermis.

RESUMEN: Caligus rogercresseyi es un copépodo que representa uno de los principales desafíos de la industria del cultivo de salmónidos en Chile, ya que afecta profusamente a la piel. Es preciso destacar que los peces en agua dulce y estuario no son afectados, a diferencia del salmón, que desde el post-smolt resulta muy parasitado cuando es trasladado al mar. Se han realizado múltiples estudios sobre el ciclo de vida del parásito y desarrollado tratamientos químicos, físicos y mecánicos para eliminarlos. Sin embargo, a la fecha, los tratamientos no han sido eficaces, lo que produce un problema permanente para el bienestar del animal. El propósito de este estudio fue el de reconocer la bioestructura de la piel de la aleta caudal del salmón del atlántico en los sitios de la interacción con chalimus. Para esto, se utilizaron 15 post-smolt infectados con Caligus y 5 post-smolt controles, sin Caligus. Los salmones fueron aportados por Fundación Chile y la experiencia se realizó en su propio centro experimental. Una vez realizada la eutanasia, mediante sobredosis del anestésico benzocaína, se obtubieron muestras de las aletas caudales, las cuales fueron fijadas en formalina al 10%, incluidas en paraplast para realizar cortes de 5 µm de espesor y teñidas con Tricrómico de Masson y PAS. Los resultados indicaron que la piel de la aleta caudal de los post-smolt afectados presentan mayor altura de la epidermis, escasa células secretoras de mucus y solución de continuidad en la epidermis. Además, la membrana basal se descontinúa y ocurre un aumento de melanomacrófagos en la dermis.

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Tiempos de traslado, características y evolución de los pacientes con infarto agudo de miocardio que requirieron derivación a Centros con Hemodinamia a más de 400 km de San Carlos de Bariloche.
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BOCIAN, JORGE LUIS

; CALANDRELLI, MATÍAS

; TREVISAN, MARIANO

; SAAVEDRA, MARÍA EMILIA

; CAMINOS, MARIO

; BONIFACIO, JUAN PABLO

Revista argentina de cardiología

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jun 2021, Volume 89 N. 3 Pages 237 - 242

Abstract: En Es | Text: En Es | PDF: En | PDF: Es

ABSTRACT Introduction: This study analyzes transfer times and complications to Hemodynamics centers (PCI-C) of patients from the prospective REGIBAR registry, including all patients suffering a first acute myocardial infarction (AMI) in San Carlos de Bariloche between June 2014 and June 2015. Methods: Among 114 hospitalized AMI cases, 25 patients were transferred to PCI-C more than 400 km from San Carlos de Bariloche. Results: Median transfer management time (TMT) for emergency referral, both by ambulance [mobile intensive care unit (MICU)] and by plane, was 480 minutes. Median travel time (TT) by MICU was 407 minutes and by air ambulance, 259 minutes. The sum of management and transfer times, that is, the total time (TOT), was 915 min by emergency MICU and 780 min by plane. The latter saved only 15% of the total travel time. Conclusions: Transfer times were longer than expected, mainly at the expense of TMT. There was a tendency to transfer those who had post-AMI angina, a higher number of ST-segment elevation leads, heart failure, but also younger patients. There were no complications during transfers.

RESUMEN Introducción: Este estudio analiza los tiempos y las complicaciones de los traslados hacia centros con Hemodinamia (C-PCI) de los pacientes del registro prospectivo REGIBAR, que incluyó a todos los pacientes que sufrieron un primer infarto agudo de miocardio (IAM) en San Carlos de Bariloche entre junio de 2014 y junio de 2015. Material y métodos: Se registraron 114 casos de IAM internados. De ellos, 25 pacientes fueron trasladados a C-PCI a más de 400 km. Resultados: La mediana de tiempo de gestión del traslado (TGT) para derivación de emergencia, tanto en ambulancia (unidad de terapia intensiva móvil, UTIM) como en avión, fue de 480 minutos. La mediana de tiempo de traslado (T) con UTIM fue de 407 minutos y con avión sanitario, de 259 minutos. La sumatoria de los tiempos de gestión y de traslado, esto es, el tiempo total (TOT), fue de 915 min con UTIM de emergencia y de 780 min en avión. Este último implicó un ahorro de solo 15% del tiempo total de traslado. Conclusiones: Los tiempos de traslado fueron más prolongados de lo esperado, principalmente a expensas del TGT. Se tendió a trasladar a quienes tuvieron angina pos-IAM, mayor cantidad de derivaciones con supradesnivel del segmento ST, insuficiencia cardíaca, pero también a los más jóvenes. No hubo complicaciones durante los traslados.

https://doi.org/10.7775/rac.es.v89.i3.20250 49 downloads

Avaliação de políticas de combate à pobreza no Brasil: aplicação do método de Controle Sintético Generalizado para o Fecop no Ceará
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Silva, Vitor Hugo Miro C.

; Mariano, Francisca Zilânia

; Campêlo, Guaracyane Lima

; França, Natália Cecilia de

; França, João Mário Santos de

; Firmiano, Marília Rodrigues

Nova Economia

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jan 2021, Volume 31 N. 1 Pages 273 - 302

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Resumo O objetivo deste artigo é estimar o impacto da criação do Fundo Estadual de Combate à Pobreza (FECOP), em 2003, sobre indicadores de pobreza no Ceará. A aplicação empírica diferencia-se pelo uso do método de Controle Sintético Generalizado, recentemente desenvolvido por Xu (2017). Com dados em nível estadual para o período de 1981 a 2014, os resultados indicam um impacto estatisticamente significativo do FECOP sobre a redução da pobreza e extrema pobreza no Ceará a partir de 2008. Adicionalmente, realizou-se uma análise comparativa dos estados utilizados na simulação do controle sintético com o Ceará, no que diz respeito ao número e valor total de benefícios do Programa Bolsa Família. O intuito é apresentar evidências de que os efeitos encontrados não estão sendo afetados pelos repasses do programa federal.

Abstract The objective of this study is to estimate the impact of the creation of the State Fund to Combat Poverty (FECOP) in 2003 on poverty indicators in Ceará. The empirical application is distinguished by the use of the Generalized Synthetic Control method recently developed by Xu (2017). With state-level data for the period 1981 to 2014, the results indicate a statistically significant impact of FECOP on poverty and extreme poverty reduction in Ceará starting in 2008. Additionally, a comparative analysis of states composing the synthetic control for Ceará was carried out, regarding the number and total value of benefits of the Program Bolsa Família. The purpose is to present evidence that the effects found are not being affected by federal program transfers.

https://doi.org/10.1590/0103-6351/5449 250 downloads

Cross-linking mass spectrometry reveals structural insights of the glutamine synthetase from Leishmania braziliensis
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de Lima, Jhenifer Yonara

; Santos, Marlon Dias Mariano

; Murakami, Mario Tyago

; Carvalho, Paulo Costa

; de Souza, Tatiana de Arruda Campos Brasil

Memórias do Instituto Oswaldo Cruz

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2021, Volume 116 elocation e210209

Abstract: En | Text: En | PDF: En

BACKGROUND Leishmaniasis is a neglected tropical disease caused by the parasite Leishmania braziliensis, commonly found in Brazil and associated with cutaneous and visceral forms of this disease. Like other organisms, L. braziliensis has an enzyme called glutamine synthetase (LbGS) that acts on the synthesis of glutamine from glutamate. This enzyme plays an essential role in the metabolism of these parasites and can be a potential therapeutic target for treating this disease. OBJECTIVES Investigate LbGS structure and generate structural models of the protein. METHODS We use the method of crosslinking mass spectrometry (XLMS) and generate structural models in silico using I-TASSER. FINDINGS 42 XLs peptides were identified, of which 37 are explained in a monomeric model with the other five indicating LbGS dimerization and pentamers interaction region. The comparison of 3D models generated in the presence and absence of XLMS restrictions probed the benefits of modeling with XLMS highlighting the inappropriate folding due to the absence of spatial restrictions. MAIN CONCLUSIONS In conclusion, we disclose the conservation of the active site and interface regions, but also unique features of LbGS showing the potential of XLMS to probe structural information and explore new drugs.

https://doi.org/10.1590/0074-02760210209

Actitudes de los Profesores de Ciencias Naturales y Ciencias Sociales hacia la Enseñanza de Competencias de Consulta en Línea y sus Factores de Fondo en el Uso del Internet
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Malebrán, Mariano Eliseo Rodríguez

; Quintanilla-Gatica, Mario Roberto

; Manzanilla, Miguel Angel

Ciência & Educação (Bauru)

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2021, Volume 27 elocation e21008

Abstract: En Es | Text: En Es | PDF: En | PDF: Es

Abstract: The attitude of the teaching staff is one of the factors affecting the integration of new technologies into the classroom. With that in mind, we ask ourselves: how do the attitudes of teachers of natural and social sciences towards teaching online consultation skills and their underlying factors influence the use of the Internet? 33 teachers from 18 public schools in a city of Chile, associated with the joint project between Chile and Finland, AKA EDU03, participated, applying a Likert questionnaire. After a multiple correspondence analysis, age was shown to be a factor associated with teachers' attitudes to teaching online consultation skills, which is an interesting finding for further research, particularly today in attitudes related to the COVID 19 pandemic, which demands greater effort from teachers in using technologies in remote teaching, assessment and learning formats.

Resumen: La actitud del profesorado es uno de los factores que inciden en la integración de las nuevas tecnologías en el aula. Desde allí nos preguntamos ¿Cómo influyen las actitudes del profesorado de ciencias naturales y ciencias sociales hacia la enseñanza de competencias de consulta en línea y sus factores de fondo en el uso del Internet? Participaron 33 profesores de 18 colegios públicos de una ciudad de Chile, asociados al proyecto conjunto entre Chile y Finlandia, AKA EDU03, aplicando un cuestionario Likert. Luego de un análisis de correspondencias múltiple, la edad se mostró como factor asociado a las actitudes de los profesores para la enseñanza de competencias de consulta en línea, lo que constituye un hallazgo interesante para profundizar en investigaciones futuras, particularmente hoy en actitudes relacionadas con la pandemia COVID 19 que demanda un mayor esfuerzo del profesorado por el uso de tecnologías en un formato remoto de enseñanza, evaluación y aprendizaje.

https://doi.org/10.1590/1516-731320210008 641 downloads

Deformación de la Aleta Caudal en Alevín de Salmón del Atlántico (Salmo salar), en Etapa de Saco Vitelino
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Rojas, Mariana

; Pellón, Mario

; Cerda, Claudio

; Ulloa, Roberto

; Suarez, Rudy

; Godoy, Marcos

; del Sol, Mariano

International Journal of Morphology

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dec 2020, Volume 38 N. 6 Pages 1631 - 1638

Abstract: En Es | Text: En Es | PDF: En | PDF: Es

SUMMARY: This study analyzes the deformity of Atlantic salmon fry (Salmo salar) caudal fin, which gives it a "pointed tail" appearance. Although at hatching specimens were normal, the deformities were detected later. The objective of this work is to describe the anatomical and histological changes of the deformed caudal fin of the Atlantic salmon fry (Salmo salar). In this analysis we used 60 specimens for laboratory analyses, to rule out the presence of viral or bacterial pathogens. Another 60 fry, developmental stage between 600 and 700 Accumulated Thermal Units (UTAs) were anesthetized with 5 % Benzocaine, fixed in 10 % formalin, weighed and measured. Of these, a group of 30 fry (15 normal and 15 deformed) underwent the Hanken and Wassersug technique to evaluate lepidotrychia or dermal rays. The remaining 30 fry (15 normal and 15 deformed) were processed using H & E / Alcián blue histochemical techniques to evaluate general histological characteristics. Additionally, immunohistochemical techniques were used to determine the location and presence of Sonic hedgehog (Shh) signal centers for lepidotrychia development. The values obtained for the quantitative variables body weight, length and width of the tail fin were described using the arithmetic mean and standard deviation. The deformity was observed from 600 UTA. Weight of deformed fry was less than normal fish, length of the fin was similar in normal and deformed fish. In contrast, width of the deformed fin was significantly reduced. The sharp fin phenotype presented a change in the conformity of the edges, lepidotrychia fractures. The epidermis presented active Shh signaling centers, but the interray blastema showed vasodilation, congestion and hemorrhages. The presentation of this case was related to sudden increases in perieclosional temperature.

RESUMEN: En este estudio se analiza una deformación que afectó la aleta caudal de los alevines de salmón del Atlántico (Salmo salar) y que les proporcionó un aspecto de “cola aguzada”. Al momento de la eclosión se observaron completamente normales pero la deformación se detectó con posterioridad. El objetivo de este trabajo es describir los cambios anatómicos e histológicos de la aleta caudal deformada del alevín de salmón del Atlántico (Salmo salar). Para esto se utilizaron 60 alevines para realizar análisis de laboratorio y descartar la presencia de patógenos virales o bacterianos. Otros 60 alevines con un estado de desarrollo entre 600 y 700 Unidades térmicas acumuladas (UTAs) fueron anestesiados con Benzocaína 5 %, fijados en formalina al 10 % pesados y medidos. De estos un grupo de 30 alevines (15 normales y 15 deformes) fueron sometidos a la técnica de Hanken y Wassersug para evaluar Lepidotriquias. Los otros 30 alevines (15 normales y 15 deformes), fueron procesados mediante las técnicas histoquímicas: H&E/azul de Alcián para evaluar las características histológicas generales. Adicionalmente se utilizó técnicas inmunohistoquímicas para reconocer la ubicación y la presencia de los centros de señales Sonic hedgehog (Shh) para la formación de Lepidotriquias. A los valores obtenidos para las variables cuantitativas peso y longitud de cuerpo, largo y ancho de aleta caudal, se les realizó estadística descriptiva y fueron sometidos a prueba de normalidad de Shapiro-Wilk. Las diferencias observadas entre peces normales y deformes, fueron analizadas mediante prueba t de Student o U de Mann Whitney, utilizando el paquete estadístico IBM SPSS 20.0. La deformación se observó desde las 600 UTA. El peso de los alevines deformes fue similar al de los peces normales (p>0,05), lo mismo sucedió con la longitud de la aleta (p>0,05). Por el contrario, el ancho de la aleta de los deformes fue muy reducida (p<0,05). El fenotipo de aleta aguzada presentó un cambio en la integridad de los bordes, fracturas de Lepidotriquias. La epidermis de la aleta caudal de los alevines deformados presentó sus centros de señalización Shh activos, pero el blastema interrayos presentó vasodilatación, congestión y hemorragias. La presentación de este caso se relacionó con incrementos bruscos de temperatura peri eclosional.

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10.

Abordaje laparoscópico simultáneo del cáncer de colon con metástasis hepáticas
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Maurette, Rafael J

; García Ejarque, Marcos D

; Ruíz, Hernán

; Bregante, Mariano L

; Bogetti, Diego J

; Cillo, Mariano

; Estefanía, Diego

; Bugallo, Fernando G

; Patrón Uriburu, Juan C

; Tyrrell, Carlos R

; Salomón, Mario C

; Pirchi, Daniel E

Revista argentina de cirugía

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dec 2019, Volume 111 N. 4 Pages 245 - 267

Abstract: En Es | Text: En Es | PDF: En | PDF: Es

Background: In patients with colorectal cancer with synchronous liver metastases (CLM), complete resection of the primary tumor with the metastases is the only option for curative treatment. Several case series have been reported but no randomized controlled trials have been published. Objective: The aim was to evaluate if the simultaneous laparoscopic resection of the primary colon tumor and liver metastases is feasible and safe. Material and methods: A retrospective study was conducted with patients with suspected CLM scheduled for simultaneous laparoscopic resection of the primary tumor of the colon and liver metastases. The preoperative and operative variables, short- and long-term outcomes and pathological variables were analyzed. Results: A total of 89 laparoscopic liver resections (LLR) were performed during the study period. In 28 patients, LLR was simultaneous with other procedures, 21 of which corresponded to laparoscopic colon resection. Mean surgical time for both procedures was 407 minutes. Mean hospital length of stay was 8 days. None of the patients died and overall morbidity rate was 71% with only one major complication. Overall survival and relapse-free survival at three years was 55.2% and 16.3%, respectively. Conclusion: This is the first publication analyzing this approach in our country. In well selected cases, the simultaneous laparoscopic approach is feasible, with low morbidity and mortality and acceptable oncological results.

Antecedentes: la hemicolectomía derecha laparoscópica con abordaje suprapúbico (HDLS) y empleo de tecnología. En los pacientes con cáncer de colon con metástasis hepáticas sincrónicas (CCMHS), la resección completa del tumor primario con las metástasis es la única opción de tratamiento con intención curativa. Se conocen varios informes de series de casos en el mundo; sin embargo, no existe ningún estudio aleatorizado controlado. Objetivo: el objetivo es evaluar la factibilidad y seguridad del abordaje laparoscópico simultáneo de la resección del tumor primario de colon y de la metástasis hepática. Material y métodos: es un estudio retrospectivo; a tal fin se recolectaron todos los pacientes con sospecha de CCMHS abordados por laparoscopia con intención de resección simultánea del tumor primario de colon con las metástasis hepáticas. Se analizaron variables preoperatorias, operatorias, resultados a corto y largo plazo, y anatomopatológicas. Resultados: en el período de estudio se realizaron 89 resecciones hepáticas laparoscópicas (RHL). En 28 pacientes se realizó â€’en forma simultánea con la RHLâ€’ otro procedimiento, en 21 de los cuales fue una colectomía laparoscópica. El tiempo quirúrgico promedio total de los dos procedimientos llegó a 407 minutos. El promedio de días de estadía hospitalaria fue de 8 días. No hubo mortalidad en la serie, y la morbilidad global fue del 71%, pero un solo caso con morbilidad mayor. La sobrevida global y la sobrevida libre de recurrencia a los 3 años fue de 55,2% y 16,3%, respectivamente. Conclusión: Esta es la primera publicación acerca del tema en nuestro país. Podemos decir que, en casos bien seleccionados, el abordaje laparoscópico simultáneo es factible de realizar, con aceptable morbimortalidad y sin comprometer los resultados oncológicos.

387 downloads

11.

A spectral multidomain penalty method solver for the numerical simulation of granular avalanches
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Trujillo-Vela, Mario Germán

; Escobar-Vargas, Jorge Alberto

; Ramos-Cañón, Alfonso Mariano

Earth Sciences Research Journal

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dec 2019, Volume 23 N. 4 Pages 317 - 329

Abstract: Es En | Text: Es En | PDF: Es | PDF: En

RESUMEN Este trabajo presenta la simulación numérica de una avalancha granular a escala experimental basada en elementos de alto orden, con el fin de evaluar el potencial de estas técnicas espectrales para resolver las leyes de conservación en geofísica. La discretización espacial de estas ecuaciones se desarrolló a través del métodos penalizado de multidominios espectrales (SMPM). Los términos temporales se discretizaron utilizando un método Runge-Kutta de preservación de estabilidad fuerte. La estabilidad del esquema numérico se garantiza con el uso de un filtro espectral y un coeficiente de presión lateral de tierras constante o regularizado. El caso de prueba es una avalancha granular que se genera en un canal rectangular de pequeña escala con gradiente topográfico. Se realizó una prueba de independencia de malla para aclarar el orden del error en la conservación de la masa producida por los tratamientos previamente mencionados. Las predicciones numéricas se comparan con las mediciones experimentales realizadas por Denlinger & Iverson (2001). Además, se analizaron las condiciones de contorno, y los parámetros como el coeficiente de presión lateral de tierras y el factor de corrección de momentum para observar su incidencia al resolver las ecuaciones del flujo granular. Este trabajo identifica las bondades y debilidades del SMPM para resolver este conjunto de ecuaciones y es posible concluir que el SMPM proporciona una solución adecuada de las ecuaciones propuestas por Iverson & Denlinger (2001). Además, las predicciones son comparables a los datos experimentales y resultados numéricos dados por otros esquemas.

ABSTRACT This work presents a high-order element-based numerical simulation of an experimental granular avalanche, in order to assess the potential of these spectral techniques to handle geophysical conservation laws. The spatial discretization of these equations was developed via the spectral multidomain penalty method (SMPM). The temporal terms were discretized using a strong-stability preserving Runge-Kutta method. Stability of the numerical scheme is ensured with the use of a spectral filter and a constant or regularized lateral earth pressure coefficient. The test case is a granular avalanche that is generated in a small-scale rectangular flume with a topographical gradient. A grid independence test was performed to clarify the order of the error in the mass conservation produced by the treatments here implemented. The numerical predictions of the granular avalanches are compared with experimental measurements performed by Denlinger and Iverson (2001). Furthermore, the boundary conditions and parameters such as lateral earth pressure coefficients and the momentum correction factor were analyzed in order to observe the incidence of these features when solving the granular flow equations. This work identifies the benefits and weaknesses of the SMPM to solve this set of equations, and thus, it is possible to conclude that the SMPM provides an appropriate solution to the granular flow equations proposed by Iverson and Denlinger (2001) and comparable predictions for the experimental data.

https://doi.org/10.15446/esrj.v23n4.77683 202 downloads

12.

National survey of blindness and visual impairment in Guatemala, 2015
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Chávez, Gloria Marina Serrano

; Barrios, Ana Rafaela Salazar de

; Pojoy, Oscar Leonel Figueroa

; Reyes, Aida del Rosario Monzón Herrera de

; Melgar, Mariano Yee

; Melgar, Juan Francisco Yee

; Régil, Mario de León

; Hernandez, Carlos Alberto Mendoza

; Chanquin, Victor Alfonso Miranda

; Diaz, Evelyn

; Lansingh, Van C.

; Limburg, Hans

; Silva, Juan Carlos

; Furtado, João M.

Arquivos Brasileiros de Oftalmologia

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mar 2019, Volume 82 N. 2 Pages 91 - 97

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO | Objetivo: Estimar a prevalência de cegueira e deficiência visual em idosos que vivem na Guatemala. Métodos: Indivíduos com idade ³50 anos foram selecionados por amostragem aleatória por conglomerados, e os participantes do estudo foram avaliados pelo método de Avaliação Rápida da Cegueira Evitável. A acuidade visual foi medida e o cristalino foi examinado. Se a acuidade visual apresentada fosse <20/60, então também foi testada com um buraco estenopeico e a fundoscopia realizada. A cegueira e a deficiência visual foram classificadas como deficiência visual moderada com acuidade visual <20/60-20/200; deficiência visual grave com acuidade visual <20/200-20/400; ou cegueira com acuidade visual <20/400. A principal causa de cegueira ou deficiência visual em cada olho foi determinada, e naqueles com catarata, as barreiras ao tratamento foram avaliadas. Resultados: O estudo incluiu 3.850 pessoas com ³50 anos de idade; 3.760 (97,7%) foram examinadas. A prevalência de cegueira ajustada à idade e ao sexo foi de 2,9% (intervalo de confiança de 95%, 2,0-3,8%), 5,2% (4,0-6,4%) deficiência visual grave e 27,6% (23,3-32,0%) deficiência visual moderada. A catarata foi a principal de cegueira (77,6%), seguida de outras doenças do segmento posterior (6,0%). Catarata causada por 79,4% de deficiência visual grave, enquanto erros refrativos não corrigidos causaram 67,9% de deficiência visual moderada. Após a cirurgia de catarata, 75% dos participantes tiveram uma acuidade de 20/200, ou melhor, e 19,0% a deficiência visual não foi melhor do que 20/200 com a correção. O custo foi a principal barreira à cirurgia de catarata (56.7%). Conclusões: A prevalência de cegueira em idosos é maior na Guatemala do que na maioria dos outros países da América Central. A maioria dos casos de cegueira e deficiência visual era evitável ou tratável. O aumento da disponibilidade de tratamento de catarata a preços acessíveis e de alta qualidade teria um impacto substancial na prevenção da cegueira.

ABSTRACT | Purpose: To estimate the prevalence of blindness and visual impairment in older adults living in Guatemala. Methods: Participants ³50 years of age were selected using random cluster sampling and evaluated using the Rapid Assessment of Avoidable Blindness method. Visual acuity was measured, and the lens was examined. If presenting visual acuity was <20/60, it was also tested with a pinhole and fundoscopy was performed. Blindness and visual impairment were classified as moderate visual impairment (presenting visual acuity <20/60 to 20/200), severe visual impairment (presenting visual acuity <20/200 to 20/400), or blindness (presenting visual acuity <20/400). The primary cause of blindness or visual impairment in each eye was determined, and if the cause was cataracts, the barriers to treatment were assessed. Results: The study included 3,850 people ³50 years of age, of whom 3,760 (97.7%) were examined. The age- and sex-adjusted prevalence of blindness was 2.9% (95% confidence interval, 2.0%-3.8%), while 5.2% (4.0%-6.4%) presented with severe visual impairment, and 27.6% (23.3%-32.0%) presented with moderate visual impairment. Cataracts were the leading cause of blindness (77.6%), followed by other posterior segment diseases (6.0%). Cataracts caused 79.4% of cases of severe visual impairment, while uncorrected refractive errors caused 67.9% of cases of moderate visual impairment. Following cataract surgery, 75% of participants had a presenting visual acuity of 20/200 or better, and in 19.0% of participants, visual acuity was not better than 20/200 with correction. Cost was the main barrier to cataract surgery (56.7%). Conclusions: The prevalence of blindness in older adults is higher in Guatemala than in most Central American countries. Most cases of blindness and visual impairment were either preventable or treatable. Increased availability of affordable, high-quality cataract treatment would have a substantial impact on blindness prevention.

https://doi.org/10.5935/0004-2749.20190029 1355 downloads

13.

Study of tissue inflammatory response in different mice strains infected by dematiaceous fungi Fonsecaea pedrosoi
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Ogawa, Marilia Marufuji

; Mariano, Mario

; Silva, Maria Regina Regis

; Enokihara, Milvia Maria Simões e Silva

; Michalany, Nilceo Schwery

; Nishikaku, Angela Satie

; Silvestre, Agenor Messias

; Tomimori, Jane

Anais Brasileiros de Dermatologia

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feb 2019, Volume 94 N. 1 Pages 29 - 36

Abstract: En | Text: En | PDF: En

Abstract: Background: Diseases caused by melanized fungi include mycetoma, chromoblastomycosis and phaeohyphomycosis. This broad clinical spectrum depends on the dynamic interactions between etiologic agent and host. The immune status of the host influences on the development of the disease, as, an exemple. phaeohyphomicosis is more frequently observed in immunocompromised patients. Objectives: Examine the histological inflammatory response induced by Fonsecaea pedrosoi in several different strains of mice (BALB/c, C57BL/6, Nude and SCID, and reconstituted Nude). Methods: Fonsecaea pedrosoi was cultivated on agar gel and a fragment of this gel was implanted subcutaneously in the abdominal region of female adult mice. After infection has been obtained, tissue fragment was studied histopathologically. Results: There were significant changes across the strains, with the nodular lesion more persistent in Nude and SCID mice, whereas in immunocompetent mice the lesion progressed to ulceration and healing. The histopathological analysis showed a significant acute inflammatory reaction which consisted mainly of neutrophils in the initial phase that was subsequently followed by a tuberculoid type granuloma in immunocompetent mice. Study limitations: There is no a suitable animal model for chromoblastomycosis. Conclusions: The neutrophilic infiltration had an important role in the containment of infection to prevent fungal spreading, including in immunodeficient mice. The fungal elimination was dependent on T lymphocytes. The re-exposure of C57BL/6 mice to Fonsecaea pedrosoi caused a delay in resolving the infection, and appearance of muriform cells, which may indicate that re-exposure to fungi, might lead to chronicity of infection.

https://doi.org/10.1590/abd1806-4841.20197326 937 downloads

14.

Sampling strategy of an epidemiological survey using a satellite image program
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Ferreira, Ticiane de Góes Mário

; Rocha, José Mariano da

; David, Silvia Cardoso de

; Boligon, Jociana

; Casarin, Maísa

; Grellmann, Alessandra Pascotini

; Marin, Janice

; Ardenghi, Thiago Machado

; Zanatta, Fabricio Batistin

; Moreira, Carlos Heitor Cunha

Revista de Saúde Pública

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2019, Volume 53 elocation 47

Abstract: En | Text: En | PDF: En

ABSTRACT OBJECTIVE To describe the sampling strategy of an epidemiological survey with the aid of satellite images, including details of the multistage probability sampling process. METHODS A probability sample of individuals living in the rural area of Rosário do Sul, state of Rio Grande do Sul, Brazil, aged 15 years old or more, was evaluated. Participants answered questionnaires (medical history, sociodemographic characteristics, habits, alcohol use, quality of life, stress, rumination, and self-perceived periodontal diseases), and were subjected to clinical oral examinations as well as anthropometric measurements (blood pressure, height, weight, abdominal and waist circumferences). Oral evaluation comprehended a complete periodontal exam at six sites per tooth, including the following assessments: furcation involvement; dental abrasion; tooth decay, including the indexing of missing and filled surfaces; O’Brien index; gingival abrasion; oral cavity and lip lesions; complete periapical radiographic exam, and use of prostheses. Besides this oral clinical approach, subgingival plaque, crevicular gingival fluid, saliva, and blood samples were collected. Examiners were trained and calibrated during previous evaluations. A pilot study allowed the logistic of the performed exams to be adjusted as needed. RESULTS Among 1,087 eligible individuals, 688 were examined (63.3%). Age, sex, and skin color data were compared to data from the last demographic census (2010) of the Brazilian Institute of Geography and Statistics, which served to validate the sampling strategy. CONCLUSIONS The careful methods used in this study, in which satellite images were used in the delimitation of epidemiological areas, ensure the quality of the estimates obtained and allow for these estimates to be used in oral health surveillance and health policies improvements.

https://doi.org/10.11606/s1518-8787.2019053000834 702 downloads

15.

Sampling strategy of an epidemiological survey using a satellite image program
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Ferreira, Ticiane de Góes Mário

; Rocha, José Mariano da

; David, Silvia Cardoso de

; Boligon, Jociana

; Casarin, Maísa

; Grellmann, Alessandra Pascotini

; Marin, Janice

; Ardenghi, Thiago Machado

; Zanatta, Fabricio Batistin

; Moreira, Carlos Heitor Cunha

Revista de Saúde Pública

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2019, Volume 53 elocation 47

Abstract: En | Text: En | PDF: En

https://doi.org/10.11606/s1518-8787.2019053000834

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