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1.
Note on the phylogenetic position of Eptesicus species in the Vespertilionidae family (Chiroptera) using Brazilian bats and RAG2 sequences
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2.
Stochastic Sensing of Organotin Compounds with Alpha-Hemolysin Nanopore AlphaHemolysin Alpha Hemolysin
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Silva, Artur A. R.
; Silva Júnior, Janilson J.
; Machado, Dijanah C.
; Pontes, Frederico J. S.
; Pol-Fachin, Laércio
; Aguiar, Juliana P.
; Rodriguesa, Claudio G.
.
Organotin compounds (OTCs) are pollutants that affect the reproduction of some marine organisms and can cause autoimmune diseases in humans. Most of the techniques used for detection and monitoring of OTCs in the environment have limitations due to several stages of sample pre-processing. Here, we demonstrated the use of the α-hemolysin (αHL) nanopore as an alternative platform for the detection of the cyhexatin and diphenyltin dichloride (DPhT) in aqueous systems. The detection process is based on the analysis of the residence times of each OTCs within the unitary nanopore, as well as, on the amplitudes of the blockages in the ionic current flowing through it. Cyhexatin and DPhT induced two and three patterns in the amplitude of the blockages in the ionic current, respectively. Residence time value of the cyhexatin inside the nanopore was higher than the value presented by the DPhT. Molecular docking was used to evaluate the interactions between OTCs and nanopore constriction, mainly identifying cation-π and hydrogen interactions. Classical atomistic molecular dynamic simulations confirm moderate aggregative behavior of these OTCs in solution in cationic form, especially for cyhexatin species. The nanopore detects OTCs at nanomolar level and it is possible to use this nanopore to monitor OTCs in aqueous systems. (OTCs humans preprocessing. preprocessing pre processing. processing pre-processing Here αhemolysin α hemolysin αHL (αHL (DPhT systems respectively constriction cationπ cation π form species
3.
1H NMR Chemical Profile and Antioxidant Activity of Eugenia punicifolia Extracts Over Seasons: A Metabolomic Pilot Study H Seasons
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Neves, Kidney O. G.
; Santos, Maria F. C.
; Mar, Josiana M.
; Pontes, Flávia L. D.
; Tormena, Claudio F.
; Chaves, Francisco C. M.
; Campos, Francinete R.
; Sanches, Edgar Aparecido
; Bezerra, Jaqueline A.
; Machado, Marcos B.
; Santos, Alan D. C.
.
Eugenia punicifolia (Kunth) DC. is a medicinal plant used to treat diseases related to oxidative processes. In this work, 1H nuclear magnetic resonance (NMR) spectroscopy and multivariate analysis have been employed to track the chemical changes and antioxidant activity of dimethyl sulfoxide (DMSO) extracts from E. punicifolia leaves over seasons. Principal component analysis (PCA) applied to 1H NMR allowed discriminating DMSO extracts from leaves collected in the dry and rainy seasons and pointed out sucrose, catechin, and epicatechin as responsible for separating dry season samples and quercetin, acid gallic, glucose, and fatty acids contributed for rainy samples grouping. Notably, antioxidant assays revealed that dry season extracts exhibited a higher radical scavenging capacity. When those compounds were submitted to partial least squares-discriminant analysis (PLS-DA) only sucrose and fatty acids presented variable importance projection (VIP) score > 1, both metabolites are related somehow to the defense mechanisms of the plant. This pilot study may suggest new experimental approaches for more effectively monitoring the spectrum-effect relationship of E. punicifolia leaf extracts. Kunth (Kunth DC processes work H (NMR (DMSO E PCA (PCA catechin quercetin gallic glucose grouping Notably capacity squaresdiscriminant squares discriminant PLSDA PLS DA (PLS-DA VIP (VIP 1 spectrumeffect spectrum effect
4.
Oral metformin for type-2 diabetes mellitus treatment in a black-tufted marmoset (Callithrix penicillata) type2 type 2 type- blacktufted black tufted Callithrix penicillata
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Duz, Janyni
; Surita, Lívia E.
; Machado, Letícia
; Costa, Priscila M.D.
; Machado, Bruna S.
; Valle, Stella F.
; Alievi, Marcelo M.
; Pöppl, Álan G.
.
RESUMO: Diabetes mellitus tipo 2 (DM2) caracteriza-se por uma combinação de defeitos na secreção de insulina e resistência periférica ao hormônio. Diversas espécies de primatas não humanos (PNH) desenvolvem DM2, sobretudo animais cativos com atividade física reduzida e alimentados incorretamente. Este trabalho descreve o tratamento de DM2 em sagui-de-tufo-preto (Callithrix penicillata), através da reformulação da dieta e administração oral de metformina. Uma fêmea adulta foi diagnosticada com DM2 após apresentar hiperglicemia e frutosaminemia elevadas associadas à glicosúria e à obesidade. Iniciou-se o uso do cloridrato de metformina (125mg/animal, VO, SID) associado ao controle de consumo alimentar com ajustes da dieta. Após 26 dias pode-se observar redução significativa de peso, adequação da glicemia e frutosaminemia, constatando resultado satisfatório da terapêutica adotada. A metformina é considerada um medicamento seguro para o tratamento de DM2, devido ao baixo risco de hipoglicemia. A base da nova dieta era batata-doce, abóbora e frutas variadas oferecidas duas vezes ao dia. Além disso, camundongos recém-nascidos descongelados, clara de ovo e pequenas porções de ração primata peletizada. No presente relato, a metformina associada a uma dieta com baixo índice glicêmico, foi eficaz para tratamento de DM2 podendo apresentar potencial terapêutico de DM2 em outros PNH. RESUMO DM (DM2 caracterizase caracteriza se hormônio PNH (PNH incorretamente saguidetufopreto sagui tufo preto Callithrix penicillata, penicillata , penicillata) obesidade Iniciouse Iniciou 125mg/animal, 125mganimal mganimal 125mg animal mg (125mg/animal VO SID podese pode peso adotada hipoglicemia batatadoce, batatadoce batata doce, doce batata-doce dia disso recémnascidos recém nascidos descongelados peletizada relato glicêmico (DM 125mg/animal
ABSTRACT: Type-2 diabetes mellitus (T2DM) is characterized by defects in insulin secretion and combined peripheral resistance to the hormone. Several non-human primates (NHP) species develop T2DM, mainly captive animals with reduced physical activity and incorrect feeding. This case report describes the T2DM treatment of a black-eared marmoset (Callithrix penicillata) by diet reformulation and metformin oral administration. An adult female was diagnosed with T2DM after hyperglycemia and high serum fructosamine associated with glycosuria and obesity. Metformin hydrochloride (125mg/animal, orally, q24h) associated with feeding intervention was started. After 26 days, a significant reduction in weight, glycemia, and serum fructosamine could be observed, showing satisfactory results for the adopted therapy. Metformin is considered a safe drug for T2DM treatment due to its low hypoglycemia risk. The new diet consisted of sweet potato, squash, and varied fruits offered twice daily. In addition, thawed-mice newborns, egg whites, and small portions of pelleted primate food. In the present report, metformin use, associated with a low glycemic index diet, was effective in treating this particular marmoset and may present a potential for T2DM treatment in other NHPs. ABSTRACT Type2 Type 2 Type- TDM T DM (T2DM hormone nonhuman non human NHP (NHP blackeared black eared Callithrix penicillata administration obesity 125mg/animal, 125mganimal mganimal 125mg animal mg (125mg/animal orally q24h qh q h started days weight glycemia observed therapy risk potato squash daily addition thawedmice thawed mice newborns whites food use NHPs 125mg/animal
5.
The high biodiversity of benthic organisms in a coastal ecosystem revealed by an integrative approach
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Amaral, A. Cecília Z.
; Cunha, Beatriz P.
; Checon, Helio H.
; Godoy, Angélica S. de
; Silva, Camila F. da
; Corte, Guilherme N.
; Nogueira, João M. de M.
; Fukuda, Marcelo V.
; Steiner, Tatiana M.
; Kawauchi, Gisele Y.
; Turra, Alexander
; Denadai, Márcia R.
; Ferreira, Luciane
; Mendonça, Joel B. de
; Tavares, Marcos
; Leite, Fosca P. P.
; Costa, Mariana F. B.
; Siqueira, Silvana G. L.
; Vieira, Leandro M.
; Dias, Gustavo M.
; Teixeira, Joyce A.
; Rocha, Rosana M.
; Gusmão, Luciana C.
; Borges, Michela
; Alitto, Renata
; Machado, Fabrizio M.
; Passos, Flávio D.
; Cunha, Carlo M.
; Simone, Luiz R.L.
; Araujo, Ana Paula G.
; Carbayo, Fernando
; Bahia, Juliana
; Bulnes, Verónica N.
; Castello-Branco, Cristiana
; Hajdu, Eduardo
; Vilas-Boas, Ana Carolina
; Garraffoni, André R. S.
; Schockaert, Ernest
; Fonseca, Gustavo
; Domenico, Maikon Di
; Curini-Galletti, Marco
; Sørensen, Martin V.
; Hochberg, Rick
; Oliveira, Ana Julia F. C. de
; Zampieri, Bruna Del B.
; Chinelatto, Roberta M.
; Migotto, Alvaro E.
.
Resumo O aumento da modificação dos habitats e da perda de espécies demanda esforços consistentes para descrever e compreender os padrões de biodiversidade. O programa BIOTA/FAPESP foi criado nesse contexto e é uma iniciativa de sucesso para promover estudos em biodiversidade e conservação no Brasil. O BIOTA/Araçá é um projeto interdisciplinar que promoveu uma avaliação detalhada da biodiversidade da Baía do Araçá, um ecossistema costeiro localizado ao Norte do estado de São Paulo, Sudeste do Brasil. A baía engloba múltiplos habitats, tais como praias, manguezais, costões rochosos, e uma planície de maré, e também fornece importantes serviços ecossistêmicos. Infelizmente, a baía está sujeita à conflitos sócio-ambientais complexos que contrastam demandas econômicas, sociais e ambientais (i.e. a expansão das atividades do porto vizinho vs. a pesca artesanal de pequena escala e a proteção da biodiversidade). O presente estudo apresenta um levantamento das espécies bentônicas que ocorrem nos diferentes habitats da Baía do Araçá, incluindo dados obtidos durante o projeto BIOTA/Araçá e de investigações realizadas anteriormente na área. As espécies bentônicas desempenham um papel importante no ambiente marinho, e estudar a diversidade desses organismos que vivem associados ao fundo é indispensável para compreender o funcionamento do meio ambiente. A macrofauna, meiofauna, e microorganismos associados aos fundos consolidado e inconsolidado foram listados, e informações adicionais foram fornecidas para cada espécie, tais como a distribuição geográfica e nos habitats. O checklist inclui 826 espécies, quase 70% registradas durante o projeto BIOTA/Araçá. Os taxa mais especiosos foram os anelídeos (225 spp.), moluscos (194 spp.), e crustáceos (177 spp.). Entre as espécies bentônicas listadas, sete são endêmicas da Baía do Araçá, 14 são consideradas ameaçadas de extinção, e sete são exploradas economicamente. A baía é a localidade tipo de vários taxa, e 11 novas espécies bentônicas foram descritas com base em espécimes amostrados durante o projeto. Este projeto mostra a importância da Baía do Araçá como um ambiente de riqueza biológica única e demonstra a necessidade de esforços para a sua conservação considerando as atuais ameaças. BIOTAFAPESP BIOTA FAPESP Brasil BIOTAAraçá Paulo praias manguezais rochosos maré ecossistêmicos Infelizmente sócioambientais sócio econômicas i.e. ie i (i.e vs . biodiversidade) área marinho macrofauna meiofauna listados espécie 82 70 225 (22 spp., spp spp. , spp.) 194 (19 177 (17 spp.. listadas 1 extinção economicamente ameaças i.e 8 7 22 (2 19 (1 17 2 (
Abstract Increasing habitat modification and species loss demand consistent efforts to describe and understand biodiversity patterns. The BIOTA/FAPESP Program was created in this context and it has been a successful initiative to promote studies on biodiversity and conservation in Brazil. The BIOTA/Araçá is an interdisciplinary project that provided a detailed evaluation of the biodiversity of Araçá Bay, a coastal seascape located on the North coast of the state of São Paulo, Southeast Brazil. The bay encompasses multiple habitats, such as beaches, mangroves, rocky shores, and a tidal flat, and provides important ecosystem services. Unfortunately, the bay is the subject of complex social-environmental conflicts that oppose economic, social, and environmental demands (i.e., the expansion of neighboring harbor activities vs. small-scale artisanal fisheries and protection of biodiversity). The present study presents a survey of the benthic species occurring in the different habitats of Araçá Bay, including data obtained during the BIOTA/Araçá project and previous assessments of the area. The benthic species play an important role in marine environments and studying the diversity of these organisms that live associated with the bottom is indispensable for comprehending the environment’s functioning. The macrofauna, meiofauna, and microorganisms associated with soft and hard bottom were listed, and additional information, such as the habitat and geographical distribution, were provided for each species. The checklist includes 826 species, almost 70% recorded during the BIOTA/Araçá project. The most speciose taxa were the annelids (225 spp.), mollusks (194 spp.), and crustaceans (177 spp.). Seven benthic species are endemic to Araçá Bay, 14 are considered threatened, and seven are economically exploited. Furthermore, the bay is the type locality of many taxa, and 11 new benthic species were described based on specimens sampled during the project. This project shows the importance of Araçá Bay as a unique biologically rich environment and highlights the need for conservation efforts in light of the current threats. patterns BIOTAFAPESP BIOTA FAPESP Brazil BIOTAAraçá Paulo beaches mangroves shores flat services Unfortunately socialenvironmental social economic i.e., ie i e (i.e. vs smallscale small scale biodiversity. . biodiversity) area s functioning macrofauna meiofauna listed information distribution 82 70 225 (22 spp., spp spp. , spp.) 194 (19 177 (17 spp.. 1 threatened exploited Furthermore threats i.e. (i.e 8 7 22 (2 19 (1 17 i.e 2 (
6.
Bone-marrow mononuclear cells and acellular human amniotic membrane improve global cardiac function without inhibition of the NLRP3 Inflammasome in a rat model of heart failure Bonemarrow Bone marrow NLRP
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TAKEJIMA, ALINE L.
; MACHADO-JÚNIOR, PAULO ANDRÉ B.
; BLUME, GUSTAVO G.
; SIMEONI, ROSSANA BAGGIO
; FRANCISCO, JULIO CESAR
; TONIAL, MURILO S.
; MARQUEZE, LUIS FELIPE B.
; NORONHA, LUCIA
; OLANDOSKI, MARCIA
; ABDELWAHID, ELTYEB
; CARVALHO, KATHERINE A.T. DE
; PINHO, RICARDO A.
; GUARITA-SOUZA, LUIZ CÉSAR
.
Abstract Recent studies have suggested that therapies with stem cells and amniotic membrane can modulate the inflammation following an ischemic injury in the heart. This study evaluated the effects of bone-marrow mononuclear cells (BMMC) and acellular human amniotic membrane (AHAM) on cardiac function and NLRP3 complex in a rat model of heart failure.On the 30th day,the echocardiographic showed improvements on ejection fraction and decreased pathological ventricular remodeling on BMMC and AHAM groups.Oxidative stress analysis was similar between the three groups,and the NLRP3 inflammasome activity were not decreased with the therapeutic use of both BMMC and AHAM,in comparison to the control group. bonemarrow bone marrow (BMMC (AHAM NLRP failureOn failure On th daythe day groupsOxidative groups Oxidative groupsand AHAMin group
7.
Exploring Urease Inhibition by Coumarin Derivatives through in silico and in vitro Methods
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Fabris, Marciéli
; Camargo, Priscila G.
; Silva, Mariana L.
; Silva, Talis U.
; Machado, Sérgio P.
; Rodrigues, Carlos R.
; Lima, Camilo H. S.
; Albuquerque, Magaly G.
; Bispo, Marcelle L. F.
.
In this report, we designed and synthesized ten N-(R-phenyl)-3-carboxamide-coumarin derivatives (2a-2j), exploring the coumarin nucleus, and an R-phenyl group as a structural scaffold and a peptide bond as a linker between them. The structure-activity relationships were investigated with different R-substituents (H, Br, NO2, Cl) in ortho, meta, or para positions from the phenyl group. Coumarins were obtained in good yields (72-95%), and in vitro screening against Canavalia ensiformis urease showed potential inhibitory percentages ranging from 42 to 65%. Half-maximal inhibitory concentration (IC50) values were determined for the best compounds 2b (R = 2-Br) and 2d (R = 4-Br). After conducting molecular docking and molecular dynamics on urease from C. ensiformis and Helicobacter pylori, potential binding modes for the most effective compounds 2b and 2d showed that these derivatives are able to interact with the crucial residue Cys592, thereby blocking the access of the urea substrate to the active site. According to density functional theory (DFT) calculations, 4-Br substitution on H. pylori urease was necessary for interacting with catalytic amino acids on the active site. report NRphenyl3carboxamidecoumarin NRphenylcarboxamidecoumarin N R 3 carboxamide 2a2j, 2a2j aj 2a 2j , j (2a-2j) nucleus Rphenyl them structureactivity structure activity Rsubstituents substituents H, H (H Br NO2 NO Cl ortho meta 7295%, 7295 72 95% 95 (72-95%) 4 65 65% Halfmaximal Half maximal IC50 IC (IC50 b 2Br 2 2-Br d 4Br. 4Br . 4-Br) C Cys592 Cys site DFT (DFT calculations NRphenyl carboxamidecoumarin (2a-2j 7295% 729 7 9 (72-95% 6 IC5 (IC5 Cys59 (72-95 (IC Cys5 (72-9 (72- (72 (7 (
8.
Short-term high-fat diet alters behavior, peripheral metabolism, and brain mitochondrial function in Swiss mice Shortterm Short term highfat high fat behavior metabolism
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MACHADO, ADRIANO EMANUEL
; REZER, PETERSON
; MANCINI, GIANNI
; LATINI, ALEXANDRA
; MOREIRA, EDUARDO LUIZ G.
.
Anais da Academia Brasileira de Ciências
- Journal Metrics
Abstract Understanding the temporal dynamics of high-fat diets (HFD) effects on behavior and metabolism is crucial for comprehending their negative impact on organisms. This study investigated the short-term effects (15, 25, and 35 days) of HFD in Swiss mice. Our findings revealed distinct behavioral and metabolic changes throughout the treatment. After 15 days of HFD, mice exhibited impaired exploratory habituation and significant increases in visceral adipose mass, fasting glucose levels, and glucose intolerance. Extending the diet to 25 days intensified the metabolic effects, resulting in compromised acquisition of recognition memory, increased body mass gain, and elevated plasma total cholesterol and triglyceride levels. After 35 days of HFD, these effects were further intensified and accompanied by anxiogenic-like responses in the open field test. Additionally, we observed a positive correlation between metabolic changes and behavioral impairments alongside prefrontal cortex mitochondrial dysfunction. In conclusion, our study reveals the temporal dynamics of behavioral and metabolic changes induced by short-term HFD in Swiss mice, highlighting the relationship between metabolic dysfunction and behavioral impairments. These findings pave the way for future research to unravel the underlying mechanisms and develop strategies to counteract the detrimental effects of HFD on behavior and metabolism. highfat high fat (HFD organisms shortterm short term 15, (15 3 treatment 1 levels intolerance 2 memory gain anxiogeniclike anxiogenic like test Additionally conclusion (1 (
9.
Physicochemical Characterization, Proximate Composition and Fatty Acid Profile of Fruits from Brazilian Northeast Agrobiodiversity Characterization
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Soares, Rafaela de Lima Gomes
; Assis, Renata Carmo de
; Mendes, Ana Erbênia Pereira
; Siqueira, Adriana Camurça Pontes
; Costa, Eveline de Alencar
; Sousa, Paulo Henrique Machado de
; Lucena, Eliseu Marlônio Pereira de
; Nascimento, Luis Gustavo Lima
; Rodrigues, Tigressa Helena Soares
; Diniz, Derlange Belizário
; Figueiredo, Raimundo Wilane de
; Maia, Carla Soraya Costa
.
Abstract The objective was to evaluate the physicochemical characteristics, nutritional composition and fatty acid profile of eleven exotic fruits in Brazil Northeast. The fruits, except pequi, presented acid pH, high moisture, low protein, low lipid and low energetic contents. Pequi is highlighted by its high protein content (2.79 g.100 g-1), lipid (13.6 g.100 g-1), carbohydrates (28.71 g.100 g-1), titratable acidity (2.75 g citric acid.100 g-1) and pH (2.54-5.19). Unpeeled and peeled jenipapo presented higher ash composition (1.26-1.38g.100 g-1), soluble solids (20.29-21.17 ºBrix) and carbohydrates (22.55-23.66 g.100 g-1) compared to others fruits. Fourteen fatty acids were quantified and classified as saturated fatty acids, monounsaturated fatty acids and polyunsaturated fatty acids. The concentrations of total fatty acids ranged from 1.92 to 1293.21 mg.100 g-1, being palmitic acid and oleic acid more prevalent. The fruits composition data indicated potential for improvement of diets, food industry and gastronomic market. characteristics Northeast pequi moisture contents 2.79 279 2 79 (2.7 g100 100 g.10 g1, g1 1 , 13.6 136 13 6 (13. 28.71 2871 28 71 (28.7 2.75 275 75 acid100 acid.10 g-1 2.545.19. 254519 2.54 5.19 . 54 5 19 (2.54-5.19) 1.261.38g.100 126138g100 1.26 1.38g.100 26 38g (1.26-1.38g.10 20.2921.17 20292117 20.29 21.17 20 29 21 17 (20.29-21.1 ºBrix 22.5523.66 22552366 22.55 23.66 22 55 23 66 (22.55-23.6 192 92 1.9 129321 1293 1293.2 mg100 mg mg.10 1, prevalent diets market 2.7 27 7 (2. g10 10 g.1 13. (13 28.7 287 (28. acid10 acid.1 g- 545 2.545.19 25451 254 2.5 519 5.1 (2.54-5.19 261 1.261.38g.10 126138g10 126 1.2 138g100 1.38g.10 (1.26-1.38g.1 2921 20.2921.1 2029211 2029 20.2 2117 21.1 (20.29-21. 5523 22.5523.6 2255236 2255 22.5 2366 23.6 (22.55-23. 9 1. 12932 129 1293. mg10 mg.1 2. (2 g. (1 28. (28 acid1 acid. 2.545.1 2545 25 51 5. (2.54-5.1 1.261.38g.1 126138g1 12 138g10 1.38g.1 (1.26-1.38g. 292 20.2921. 202921 202 20. 211 21. (20.29-21 552 22.5523. 225523 225 22. 236 23. (22.55-23 mg1 mg. ( 2.545. (2.54-5. 1.261.38g. 126138g 138g1 1.38g. (1.26-1.38g 20.2921 20292 (20.29-2 22.5523 22552 (22.55-2 2.545 (2.54-5 1.261.38g 138g 1.38g 20.292 (20.29- 22.552 (22.55- (2.54- (20.29 (22.55 (2.54 (20.2 (22.5 (2.5 (20. (22. (20 (22
10.
Osmotic regulators in cowpea beans plants under water deficiency
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Martins, J. T. S.
; Costa, T. C.
; Machado, L. C.
; Ferreira, R. L. C.
; Nascimento, V. R.
; Braga, D. G.
; Brito, A. E. A.
; Nogueira, G. A. S.
; Souza, L. C.
; Medeiros, J. C. A.
; Silva, T. M.
; Jesus, K. M.
; Freitas, J. M. N.
; Okumura, R. S.
; Oliveira Neto, C. F.
.
Resumo O feijão-caupi é uma planta leguminosa pertencente à família das Fabaceae cultivada nas regiões Norte e Nordeste do Brasil, com potencial produtivo. Entre os fatores abióticos, a deficiência hídrica é uma das principais limitações ambientais que influenciam a produção agrícola no mundo. O objetivo do trabalho foi estudar o conteúdo relativo de água e os osmorreguladores de plantas de feijão-caupi submetidas ao estresse hídrico. O experimento foi conduzido em casa de vegetação da Universidade Federal Rural da Amazônia (UFRA, Belém, PA), foram utilizadas plantas de feijão-caupi BR-17 Gurguéia Vigna unguiculata (L.) Walp. O delineamento experimental foi inteiramente casualizado (DIC) em esquema fatorial 2 × 2, duas condições hídricas (controle e déficit hídrico) e dois tempos de estresse (quatro e seis dias de suspensão hídrica), com 7 repetições, totalizando 28 unidades experimentais. O estresse hídrico afetou as plantas, provocando redução no conteúdo relativos de água (69,98%), amido (12,84% nas folhas e 23,48% nas raízes) e carboidratos (84,34%), e aumento na glicina-betaína, sacarose (114,11% nas folhas e 18,71% nas raízes) e prolina (358,86%) no tempo 2. O conteúdo relativo de água foi afetado negativamente pelas condições hídricas, com diminuição em relação à interação da parte aérea e do sistema radicular. Diante disso, notou-se maiores respostas metabólicas nas plantas que foram submetidas ao tratamento de estresse no tempo 2 (6 dias). feijãocaupi feijão caupi Brasil produtivo abióticos mundo UFRA, UFRA (UFRA Belém PA, PA , PA) BR17 BR 17 BR-1 L. L (L. Walp DIC (DIC controle quatro hídrica, hídrica) repetições experimentais 69,98%, 6998 69,98% 69 98 (69,98%) 12,84% 1284 12 84 (12,84 2348 23 48 23,48 raízes 84,34%, 8434 84,34% 34 (84,34%) glicinabetaína, glicinabetaína glicina betaína, betaína glicina-betaína 114,11% 11411 114 11 (114,11 1871 18 71 18,71 358,86% 35886 358 86 (358,86% radicular disso notouse notou se 6 ( dias. . dias) BR1 1 BR- (L 699 69,98 9 (69,98% 12,84 128 8 (12,8 234 4 23,4 843 84,34 3 (84,34% 114,11 1141 (114,1 187 18,7 358,86 3588 35 (358,86 69,9 (69,98 12,8 (12, 23, 84,3 (84,34 114,1 (114, 18, 358,8 (358,8 69, (69,9 12, (12 84, (84,3 114, (114 358, (358, (69, (1 (84, (11 (358 (69 (84 (35 (8 (3
Abstract Cowpea is a leguminous plant belonging to the fabaceae family cultivated in the North and Northeast regions of Brazil, with productive potential. Among the abiotic factors, water deficiency is one of the main environmental limitations that influence agricultural production in the world. The objective of this work was to study the relative water content and osmoregulators of cowpea plants subjected to water stress. The experiment was carried out in a greenhouse at the Universidade Federal Rural da Amazônia (UFRA, Belém, PA), cowpea plants BR-17 Gurguéia Vigna unguiculata (L.) Walp were used. The experimental design was completely randomized (DIC) in a 2 × 2 factorial scheme, two water conditions (control and water deficit) and two times of stress (four and six days of water suspension), with 7 replications, totaling 28 experimental units. The water deficit affected plants, causing a reduction in relative water content (69.98%), starch (12.84% in leaves and 23.48% in roots) and carbohydrates (84.34%), and an increase in glycine-betaine, sucrose (114.11% in leaves and 18.71% in roots) and proline (358.86%) at time 2. The relative water content was negatively affected by water conditions, with a decrease in relation to the interaction of the aerial part and the root system. Therefore, greater metabolic responses were noted in plants that were subjected to stress treatment at time 2 (6 days). Brazil potential factors world UFRA, UFRA (UFRA Belém PA, PA , PA) BR17 BR 17 BR-1 L. L (L. used DIC (DIC scheme control four suspension, suspension suspension) replications units 69.98%, 6998 69.98% 69 98 (69.98%) 12.84% 1284 12 84 (12.84 2348 23 48 23.48 roots 84.34%, 8434 84.34% 34 (84.34%) glycinebetaine, glycinebetaine glycine betaine, betaine glycine-betaine 114.11% 11411 114 11 (114.11 1871 18 71 18.71 358.86% 35886 358 86 (358.86% system Therefore 6 ( days. . days) BR1 1 BR- (L 699 69.98 9 (69.98% 12.84 128 8 (12.8 234 4 23.4 843 84.34 3 (84.34% 114.11 1141 (114.1 187 18.7 358.86 3588 35 (358.86 69.9 (69.98 12.8 (12. 23. 84.3 (84.34 114.1 (114. 18. 358.8 (358.8 69. (69.9 12. (12 84. (84.3 114. (114 358. (358. (69. (1 (84. (11 (358 (69 (84 (35 (8 (3
11.
EDUCAÇÃO QUÍMICA PARA UM FUTURO SUSTENTÁVEL: DESVENDANDO A CINÉTICA QUÍMICA DA PIRÓLISE DO RESÍDUO DA CULTURA DE PIMENTA-DO-REINO (Piper Nigrum L.) PARA A POTENCIAL PRODUÇÃO DE BIO-ÓLEO SUSTENTÁVEL PIMENTADOREINO PIMENTA REINO Piper L. L BIOÓLEO BIO ÓLEO
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Welsing, Gyovana L.
; Damm, Bruna M.
; Lelis, Maria F. F.
; Machado, Marta A.
; Moura, Paulo R. G.
.
The Sustainable Development Goals (SDGs) provide a comprehensive framework to guide actions towards a more sustainable future, emphasizing the need for sustainable production and consumption patterns (SDG 12), urgent measures to combat climate change and its impacts (SDG 13), and ensuring inclusive, equitable, and quality education (SDG 4). In this context, environmental education (EE) becomes crucial to empower present and future generations to address complex environmental challenges. Promoting EE can be enhanced through Chemistry education, encouraging the use of biomass as a primary energy source, a significant ally in addressing the SDGs. This study integrates experimental and educational chemical knowledge on the pyrolysis reaction of black pepper waste, aiming at higher education in Chemistry in an Environmental Chemistry class at Federal University of Espírito Santo. It aims to provide an in-depth understanding of chemical kinetics, connected to sustainable development through the theoretical-methodological strategy of problematized experimental activity (PEA). The developed PEA comprised five theoretical-experimental classes, including thermogravimetric analysis (TGA) simulation and pyrolysis furnace testing. The results indicate significant learning in Chemical Kinetics and themes related to the pyrolysis process associated with sustainable development, highlighting the contribution of the utilized PEA. SDGs (SDGs SDG 12, 12 , 12) 13, 13 13) inclusive equitable 4. 4 . 4) context (EE challenges source waste Santo indepth depth kinetics theoreticalmethodological theoretical methodological (PEA) theoreticalexperimental classes TGA (TGA testing 1 (PEA
12.
Adequação do Consumo de Ácidos Graxos entre Pacientes em Prevenção Cardiovascular Secundária
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Marcadenti, Aline
; Machado, Rachel H. Vieira
; Santos, Renato Hideo Nakagawa
; Kasai, Caio Cesar dos Santos
; Kovacs, Cristiane
; Bello, Annie
; de Matos, Cristina H.
; Bertacco, Renata Torres Abib
; Souza, Gabriela C.
; Schirmann, Gabriela da S.
; Nagano, Francisca Eugenia Zaina
; Poloni, Soraia
; Kik, Raquel Milani El
; Feres, Naoel Hassan
; Rodrigues, Isa G.
; Sousa, Antônio Carlos Sobral
; Pinheiro, Josilene M. F.
; Vasconcelos, Sandra Mary Lima
; Carlos, Daniele Maria de Oliveira
; Souza, Viviane Sahade
; Gomes, Adriana Barros
; Figueiredo Neto, José Albuquerque de
; Moriguchi, Emilio Hideyuki
; Izar, Maria Cristina
; Pinto, Sônia Lopes
; Bressan, Josefina
; de Souza, Simone Raimondi
; Kumbier, Magali C.
; de Araújo, Celme Barroncas Passos
; Torreglosa, Camila R.
; Weber, Bernardete
; Bersch-Ferreira, Ângela Cristine
.
Resumo Fundamento: A adesão à uma alimentação adequada em macronutrientes é fundamental para a prevenção secundária de doenças cardiovasculares. Objetivo: Avaliar a prevalência de adesão às recomendações de consumo de ácidos graxos para prevenção e tratamento de doenças cardiovasculares, e estimar se a presença de determinados fatores de risco cardiovascular estaria associada à adesão. Métodos: Estudo transversal com os dados de linha de base de 2358 participantes do estudo "Brazilian Cardioprotective Nutritional Program Trial". Dados de consumo alimentar, e fatores de risco cardiovascular foram avaliados. Foi considerada, de acordo com a Sociedade Brasileira de Cardiologia, uma ingestão adequada de ácidos graxos poli-insaturados (AGPI) ≥10% do consumo total de energia diária, para ácidos graxos monoinsaturados (AGM), 20% e para ácidos graxos saturados (AGS), <7%. Na análise estatística foi considerando nível de significância de 5%. Resultados: Nenhum participante aderiu a todas as recomendações de forma simultânea e mais da metade (1482 [62,9%]) não aderiu a nenhuma recomendação. A adesão exclusivamente à recomendação de AGS foi a mais prevalente, sendo cumprida por 659 (28%) dos participantes, seguida da adesão exclusivamente à recomendação de AGP (178 [7,6%]) e de AGM (5 [0,2%]). Não houve associação entre o número de comorbidades e a adesão às recomendações nutricionais (p =0,269). Os participantes da região Nordeste do país apresentaram maior proporção de adesão às recomendações para consumo de AGS (38,42%), e menor para ingestão de AGPI (3,52%) (p <0,001) em comparação às demais. Conclusões: Na amostra avaliada, evidenciou-se baixa adesão às recomendações nutricionais para consumo de ácidos graxos. Fundamento cardiovasculares Objetivo Métodos 235 Brazilian Trial. Trial . Trial" alimentar avaliados considerada Cardiologia poliinsaturados poli insaturados (AGPI 10 ≥10 diária AGM, , (AGM) 20 AGS, (AGS) 7 <7% 5 5% Resultados 1482 (148 62,9% 629 62 9 [62,9%] prevalente 65 28% 28 (28% 178 (17 7,6% 76 6 [7,6%] ( 0,2%. 02 0,2% 0 2 [0,2%]) p =0,269. 0269 =0,269 269 =0,269) 38,42%, 3842 38,42% 38 42 (38,42%) 3,52% 352 3 52 (3,52% <0,001 0001 001 demais Conclusões avaliada evidenciouse evidenciou 23 1 ≥1 (AGM (AGS <7 148 (14 62,9 [62,9% (28 17 (1 7,6 [7,6% 0,2 [0,2%] 026 =0,26 26 384 38,42 4 (38,42% 3,52 35 (3,52 <0,00 000 00 ≥ < 14 62, [62,9 (2 7, [7,6 0, [0,2% =0,2 38,4 (38,42 3,5 (3,5 <0,0 [62, [7, [0,2 =0, 38, (38,4 3, (3, <0, [62 [7 [0, =0 (38, (3 <0 [6 [ [0 = (38
Background: Adhering to a diet adequate in macronutrients is crucial for the secondary prevention of cardiovascular diseases. Objective: To assess the prevalence of adherence to recommendations for the consumption of dietary fatty acids for the prevention and treatment of cardiovascular diseases and to estimate whether the presence of certain cardiovascular risk factors would be associated with adherence. Methods: Cross-sectional study using baseline data from 2,358 participants included in the "Brazilian Cardioprotective Nutritional Program Trial". Dietary intake and cardiovascular risk factors were assessed. Adequate intake of polyunsaturated fatty acids (PUFA) was considered as ≥10% of total daily energy intake; for monounsaturated fatty acids (MUFA), 20%; and for saturated fatty acids (SFA), <7% according to the Brazilian Society of Cardiology. A significance level of 5% was considered in the statistical analysis. Results: No participant adhered to all recommendations simultaneously, and more than half (1,482 [62.9%]) did not adhere to any recommendation. Adherence exclusively to the SFA recommendation was the most prevalent, fulfilled by 659 (28%) participants, followed by adherence exclusively to the PUFA (178 [7.6%]) and MUFA (5 [0.2%]) recommendations. There was no association between the number of comorbidities and adherence to nutritional recommendations (p = 0.269). Participants from the Brazilian Northeast region showed a higher proportion of adherence to SFA consumption recommendations (38.42%) and lower adherence to PUFA intake (3.52%) (p <0.001) compared to other regions. Conclusions: Among the evaluated sample, there was low adherence to nutritional recommendations for dietary fatty acid consumption. Background Objective Methods Crosssectional Cross sectional 2358 2 358 2,35 Trial. Trial . Trial" assessed (PUFA 10 ≥10 MUFA, , (MUFA) 20% 20 SFA, (SFA) 7 <7 Cardiology 5 analysis Results simultaneously 1,482 1482 1 482 (1,48 62.9% 629 62 9 [62.9%] prevalent 65 28% 28 (28% 178 (17 7.6% 76 6 [7.6%] ( 0.2% 02 0 [0.2%] p 0.269. 0269 0.269 269 0.269) 38.42% 3842 38 42 (38.42% 3.52% 352 3 52 (3.52% <0.001 0001 001 regions Conclusions sample 235 35 2,3 ≥1 (MUFA (SFA < 1,48 148 48 (1,4 62.9 [62.9% (28 17 (1 7.6 [7.6% 0.2 [0.2% 026 0.26 26 38.42 384 4 (38.42 3.52 (3.52 <0.00 000 00 23 2, ≥ 1,4 14 (1, 62. [62.9 (2 7. [7.6 0. [0.2 38.4 (38.4 3.5 (3.5 <0.0 1, [62. [7. [0. 38. (38. 3. (3. <0. [62 [7 [0 (38 (3 <0 [6 [
13.
The effects of isocaloric diets derived from different lipid sources on zebrafish
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Peppi, P. F.
; Faria, C. A.
; Machado, J. P. C.
; Virote, B. C. R.
; Carneiro, W. F.
; Solis-Murgas, L. D.
; Portz, L.
; Santos, C. R.
; Campos, L. R. S.
; Lira, G. A.
; Lima, E. M. M.
; Bicudo, A. J. A.
; Barreto-Vianna, A. R. C.
.
Resumo Caracterizar os efeitos da ingestão de gordura saturada na saúde metabólica e suas mudanças continua sendo um desafio importante. Dietas lipídicas, provenientes de diferentes fontes, variam amplamente em seus efeitos fisiológicos na saúde; portanto, é importante considerar a fonte lipídica específica consumida. O objetivo do estudo foi avaliar o efeito da imposição de dietas isocalóricas com diferentes fontes lipídicas (óleo de peixe/gordura suína) em zebrafish e descrever como parâmetros metabólicos, morfológicos e comportamentais podem se manifestar nesses peixes. Quarenta peixes fêmeas adultas foram utilizados para o experimento. Divididos em grupo Controle (C), alimentado com ração com ácido graxo insaturados e grupo ácido graxo saturado (Sat). Eles receberam comida três vezes ao dia, durante o período de 11 semanas. Os resultados mostraram que os animais no grupo Sat tiveram aumento de peso corporal, com diferença em relação ao grupo C, a partir da terceira semana da dieta até o final do experimento. No final da última semana, o grupo Sat teve um peso corporal 32% maior (P=0,0182) do que o peso do grupo controle. O consumo de uma dieta rica em ácidos graxos saturados não gerou sinais relacionados ao estresse e ansiedade em zebrafish. Houve um aumento na glicemia em T60 e T120, com diferença estatisticamente significativa entre os dois momentos. Animais no grupo Sat mostraram um aumento (P=0,0086) na esteatose hepática em comparação com animais no grupo controle. Os resultados obtidos sobre a relação entre a dieta e as alterações metabólicas são fundamentais para garantir a compreensão e o tratamento adequado desses problemas. portanto consumida óleo peixegordura peixe suína metabólicos experimento C , (C) Sat. . (Sat) dia 1 semanas 32 P=0,0182 P00182 P 0 0182 (P=0,0182 controle T T6 T120 momentos P=0,0086 P00086 0086 (P=0,0086 problemas (C (Sat 3 P=0,018 P0018 018 (P=0,018 T12 P=0,008 P0008 008 (P=0,008 P=0,01 P001 01 (P=0,01 T1 P=0,00 P000 00 (P=0,00 P=0,0 P00 (P=0,0 P=0, P0 (P=0, P=0 (P=0 P= (P= (P
Abstract Characterizing the effects of saturated fat intake on metabolic health and its changes remains a major challenge. Lipid diets, from different sources, vary widely in their physiological effects on health; therefore, it is important to consider the specific lipid source consumed. The objective of the study was to evaluate the effect of the imposition of isocaloric diets with different lipid sources in zebrafish (fish oil/pork lard). Depicting how metabolic, morphological and behavioral parameters might express themselves in these fishes. Forty adult female fishes were used for the experiment. The animals were divided into a control group (C), fed with unsaturated fatty acid diet, and a saturated fatty acid group (Sat). They received food three times a day, during the 11-week period. The results showed that animals in the Sat group had increased body weight, with a difference relative to the C group, from the third week of diet until the end of the experiment. At the end of the last week, the Sat group had a body weight 32% higher (P=0.0182) than the body weight of the control group. The consumption of a diet rich in saturated fatty acids did not generate signs related to stress and anxiety in zebrafish. There was an increase in glycemia at T60 and T120, with a statistically significant difference between the two moments. Animals in the Sat group showed an increase (P=0.0086) in hepatic steatosis compared to animals in the control group. The results obtained on the relationship between diet and metabolic changes are fundamental to ensure the understanding and appropriate treatment of these problems. challenge therefore consumed fish oilpork oil pork lard. lard . lard) experiment C, , (C) Sat. (Sat) day 11week 11 period 32 P=0.0182 P00182 P 0 0182 (P=0.0182 T T6 T120 moments P=0.0086 P00086 0086 (P=0.0086 problems (C (Sat 1 3 P=0.018 P0018 018 (P=0.018 T12 P=0.008 P0008 008 (P=0.008 P=0.01 P001 01 (P=0.01 T1 P=0.00 P000 00 (P=0.00 P=0.0 P00 (P=0.0 P=0. P0 (P=0. P=0 (P=0 P= (P= (P
14.
Catálogo Taxonômico da Fauna do Brasil: Setting the baseline knowledge on the animal diversity in Brazil Brasil
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Boeger, Walter A.
; Valim, Michel P.
; Zaher, Hussam
; Rafael, José A.
; Forzza, Rafaela C.
; Percequillo, Alexandre R.
; Serejo, Cristiana S.
; Garraffoni, André R.S.
; Santos, Adalberto J.
; Slipinski, Adam
; Linzmeier, Adelita M.
; Calor, Adolfo R.
; Garda, Adrian A.
; Kury, Adriano B.
; Fernandes, Agatha C.S.
; Agudo-Padrón, Aisur I.
; Akama, Alberto
; Silva Neto, Alberto M. da
; Burbano, Alejandro L.
; Menezes, Aleksandra
; Pereira-Colavite, Alessandre
; Anichtchenko, Alexander
; Lees, Alexander C.
; Bezerra, Alexandra M.R.
; Domahovski, Alexandre C.
; Pimenta, Alexandre D.
; Aleixo, Alexandre L.P.
; Marceniuk, Alexandre P.
; Paula, Alexandre S. de
; Somavilla, Alexandre
; Specht, Alexandre
; Camargo, Alexssandro
; Newton, Alfred F.
; Silva, Aline A.S. da
; Santos, Aline B. dos
; Tassi, Aline D.
; Aragão, Allan C.
; Santos, Allan P.M.
; Migotto, Alvaro E.
; Mendes, Amanda C.
; Cunha, Amanda
; Chagas Júnior, Amazonas
; Sousa, Ana A.T. de
; Pavan, Ana C.
; Almeida, Ana C.S.
; Peronti, Ana L.B.G.
; Henriques-Oliveira, Ana L.
; Prudente, Ana L.
; Tourinho, Ana L.
; Pes, Ana M.O.
; Carmignotto, Ana P.
; Wengrat, Ana P.G. da Silva
; Dornellas, Ana P.S.
; Molin, Anamaria Dal
; Puker, Anderson
; Morandini, André C.
; Ferreira, André da S.
; Martins, André L.
; Esteves, André M.
; Fernandes, André S.
; Roza, André S.
; Köhler, Andreas
; Paladini, Andressa
; Andrade, Andrey J. de
; Pinto, Ângelo P.
; Salles, Anna C. de A.
; Gondim, Anne I.
; Amaral, Antonia C.Z.
; Rondón, Antonio A.A.
; Brescovit, Antonio
; Lofego, Antônio C.
; Marques, Antonio C.
; Macedo, Antonio
; Andriolo, Artur
; Henriques, Augusto L.
; Ferreira Júnior, Augusto L.
; Lima, Aurino F. de
; Barros, Ávyla R. de A.
; Brito, Ayrton do R.
; Romera, Bárbara L.V.
; Vasconcelos, Beatriz M.C. de
; Frable, Benjamin W.
; Santos, Bernardo F.
; Ferraz, Bernardo R.
; Rosa, Brunno B.
; Sampaio, Brunno H.L.
; Bellini, Bruno C.
; Clarkson, Bruno
; Oliveira, Bruno G. de
; Corrêa, Caio C.D.
; Martins, Caleb C.
; Castro-Guedes, Camila F. de
; Souto, Camilla
; Bicho, Carla de L.
; Cunha, Carlo M.
; Barboza, Carlos A. de M.
; Lucena, Carlos A.S. de
; Barreto, Carlos
; Santana, Carlos D.C.M. de
; Agne, Carlos E.Q.
; Mielke, Carlos G.C.
; Caetano, Carlos H.S.
; Flechtmann, Carlos H.W.
; Lamas, Carlos J.E.
; Rocha, Carlos
; Mascarenhas, Carolina S.
; Margaría, Cecilia B.
; Waichert, Cecilia
; Digiani, Celina
; Haddad, Célio F.B.
; Azevedo, Celso O.
; Benetti, Cesar J.
; Santos, Charles M.D. dos
; Bartlett, Charles R.
; Bonvicino, Cibele
; Ribeiro-Costa, Cibele S.
; Santos, Cinthya S.G.
; Justino, Cíntia E.L.
; Canedo, Clarissa
; Bonecker, Claudia C.
; Santos, Cláudia P.
; Carvalho, Claudio J.B. de
; Gonçalves, Clayton C.
; Galvão, Cleber
; Costa, Cleide
; Oliveira, Cléo D.C. de
; Schwertner, Cristiano F.
; Andrade, Cristiano L.
; Pereira, Cristiano M.
; Sampaio, Cristiano
; Dias, Cristina de O.
; Lucena, Daercio A. de A.
; Manfio, Daiara
; Amorim, Dalton de S.
; Queiroz, Dalva L. de
; Queiroz, Dalva L. de
; Colpani, Daniara
; Abbate, Daniel
; Aquino, Daniel A.
; Burckhardt, Daniel
; Cavallari, Daniel C.
; Prado, Daniel de C. Schelesky
; Praciano, Daniel L.
; Basílio, Daniel S.
; Bená, Daniela de C.
; Toledo, Daniela G.P. de
; Takiya, Daniela M.
; Fernandes, Daniell R.R.
; Ament, Danilo C.
; Cordeiro, Danilo P.
; Silva, Darliane E.
; Pollock, Darren A.
; Muniz, David B.
; Gibson, David I.
; Nogueira, David S.
; Marques, Dayse W.A.
; Lucatelli, Débora
; Garcia, Deivys M.A.
; Baêta, Délio
; Ferreira, Denise N.M.
; Rueda-Ramírez, Diana
; Fachin, Diego A.
; Souza, Diego de S.
; Rodrigues, Diego F.
; Pádua, Diego G. de
; Barbosa, Diego N.
; Dolibaina, Diego R.
; Amaral, Diogo C.
; Chandler, Donald S.
; Maccagnan, Douglas H.B.
; Caron, Edilson
; Carvalho, Edrielly
; Adriano, Edson A.
; Abreu Júnior, Edson F. de
; Pereira, Edson H.L.
; Viegas, Eduarda F.G.
; Carneiro, Eduardo
; Colley, Eduardo
; Eizirik, Eduardo
; Santos, Eduardo F. dos
; Shimbori, Eduardo M.
; Suárez-Morales, Eduardo
; Arruda, Eliane P. de
; Chiquito, Elisandra A.
; Lima, Élison F.B.
; Castro, Elizeu B. de
; Orlandin, Elton
; Nascimento, Elynton A. do
; Razzolini, Emanuel
; Gama, Emanuel R.R.
; Araujo, Enilma M. de
; Nishiyama, Eric Y.
; Spiessberger, Erich L.
; Santos, Érika C.L. dos
; Contreras, Eugenia F.
; Galati, Eunice A.B.
; Oliveira Junior, Evaldo C. de
; Gallardo, Fabiana
; Hernandes, Fabio A.
; Lansac-Tôha, Fábio A.
; Pitombo, Fabio B.
; Dario, Fabio Di
; Santos, Fábio L. dos
; Mauro, Fabio
; Nascimento, Fabio O. do
; Olmos, Fabio
; Amaral, Fabio R.
; Schunck, Fabio
; Godoi, Fábio S. P. de
; Machado, Fabrizio M.
; Barbo, Fausto E.
; Agrain, Federico A.
; Ribeiro, Felipe B.
; Moreira, Felipe F.F.
; Barbosa, Felipe F.
; Silva, Fenanda S.
; Cavalcanti, Fernanda F.
; Straube, Fernando C.
; Carbayo, Fernando
; Carvalho Filho, Fernando
; Zanella, Fernando C.V.
; Jacinavicius, Fernando de C.
; Farache, Fernando H.A.
; Leivas, Fernando
; Dias, Fernando M.S.
; Mantellato, Fernando
; Vaz-de-Mello, Fernando Z.
; Gudin, Filipe M.
; Albuquerque, Flávio
; Molina, Flavio B.
; Passos, Flávio D.
; Shockley, Floyd W.
; Pinheiro, Francielly F.
; Mello, Francisco de A.G. de
; Nascimento, Francisco E. de L.
; Franco, Francisco L.
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; Duarte, José M. Barbanti
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; Mermudes, José R.M.
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; Santos, Josenilson R. dos
; Câmara, Josenir T.
; Teixeira, Joyce A.
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; Duarte, Marcelo
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; Nogueira, Marcelo
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; Melo, Marcelo R.S. de
; Carvalho, Marcelo R. de
; Tavares, Marcelo T.
; Kitahara, Marcelo V.
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; Botelho, Marcia J.C.
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; Borges-Martins, Márcio
; Felix, Márcio
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; Bologna, Marco A.
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; Santos, Marcus T.T.
; Domingues, Marcus V.
; Sallum, Maria A.M.
; Digiani, María C.
; Santarém, Maria C.A.
; Nascimento, Maria C. do
; Becerril, María de los A.M.
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; Passos, Maria I. da S. dos
; Felippe-Bauer, Maria L.
; Cherman, Mariana A.
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; Bartz, Marie L.C.
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; Cupello, Mario
; Martins, Marlúcia B.
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; Rocha, Matheus dos S.
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; Mincarone, Michael M.
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; Monné, Miguel A.
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; Fernandez, Monica A.
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; Benaim, Natalia P.
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ABSTRACT The limited temporal completeness and taxonomic accuracy of species lists, made available in a traditional manner in scientific publications, has always represented a problem. These lists are invariably limited to a few taxonomic groups and do not represent up-to-date knowledge of all species and classifications. In this context, the Brazilian megadiverse fauna is no exception, and the Catálogo Taxonômico da Fauna do Brasil (CTFB) (http://fauna.jbrj.gov.br/), made public in 2015, represents a database on biodiversity anchored on a list of valid and expertly recognized scientific names of animals in Brazil. The CTFB is updated in near real time by a team of more than 800 specialists. By January 1, 2024, the CTFB compiled 133,691 nominal species, with 125,138 that were considered valid. Most of the valid species were arthropods (82.3%, with more than 102,000 species) and chordates (7.69%, with over 11,000 species). These taxa were followed by a cluster composed of Mollusca (3,567 species), Platyhelminthes (2,292 species), Annelida (1,833 species), and Nematoda (1,447 species). All remaining groups had less than 1,000 species reported in Brazil, with Cnidaria (831 species), Porifera (628 species), Rotifera (606 species), and Bryozoa (520 species) representing those with more than 500 species. Analysis of the CTFB database can facilitate and direct efforts towards the discovery of new species in Brazil, but it is also fundamental in providing the best available list of valid nominal species to users, including those in science, health, conservation efforts, and any initiative involving animals. The importance of the CTFB is evidenced by the elevated number of citations in the scientific literature in diverse areas of biology, law, anthropology, education, forensic science, and veterinary science, among others. publications problem uptodate up date classifications context exception (CTFB http//fauna.jbrj.gov.br/, httpfaunajbrjgovbr http //fauna.jbrj.gov.br/ , jbrj gov br (http://fauna.jbrj.gov.br/) 2015 Brazil 80 specialists 1 2024 133691 133 691 133,69 125138 125 138 125,13 82.3%, 823 82 3 (82.3% 102000 102 000 102,00 7.69%, 769 7 69 (7.69% 11000 11 11,00 . 3,567 3567 567 (3,56 2,292 2292 2 292 (2,29 1,833 1833 833 (1,83 1,447 1447 447 (1,44 1000 1,00 831 (83 628 (62 606 (60 520 (52 50 users science health biology law anthropology education others http//fauna.jbrj.gov.br/ faunajbrjgovbr //fauna.jbrj.gov.br (http://fauna.jbrj.gov.br/ 201 8 202 13369 13 133,6 12513 12 125,1 82.3% (82.3 10200 10 00 102,0 7.69% 76 6 (7.69 1100 11,0 3,56 356 56 (3,5 2,29 229 29 (2,2 1,83 183 83 (1,8 1,44 144 44 (1,4 100 1,0 (8 62 (6 60 52 (5 5 http//fauna.jbrj.gov.br (http://fauna.jbrj.gov.br 20 1336 133, 1251 125, 82.3 (82. 1020 0 102, 7.69 (7.6 110 11, 3,5 35 (3, 2,2 22 (2, 1,8 18 (1, 1,4 14 4 ( 82. (82 7.6 (7. 3, (3 2, (2 (1 7. (7
15.
Trypanosoma cruzi RNA-binding protein DRBD3: perinuclear foci formation during benznidazole exposure RNAbinding RNA binding DRBD3 DRBD
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CHAME, DANIELA F.
; LAET-SOUZA, DANIELA DE
; VIEIRA, HELAINE G. S.
; TAHARA, ERICH B.
; MACEDO, ANDREA MARA
; MACHADO, CARLOS RENATO
; FRANCO, GLÓRIA REGINA
.
Abstract Benznidazole (BZ) is the trypanocidal compound of choice for Chagas disease, a neglected tropical disease in the Americas. However, this drug often fails to cure the infection. The regulation of gene expression in Trypanosoma cruzi, the causative agent of Chagas disease, is based on post-transcriptional mechanisms. When environmental changes cause translational arrest, RNA-binding proteins, and their target mRNAs assemble into cytoplasmic bodies, known as RNA granules, which act as RNA sorting centers. We have characterized the T. cruzi RNA-binding protein DRBD3, which has two RRMs domains, and a C-terminal low-complexity sequence rich in proline and glutamines. Using a tagged form of TcDRBD3 (rTcDRBD3), we showed that this protein resides in the cytoplasm, but localizes into perinuclear cytoplasmic foci after BZ exposure. RNA staining after BZ also showed that this molecule accumulates into perinuclear cytoplasmic foci. Moreover, BZ and puromycin treatment enhanced the colocalization of rTcDRBD3 and RNA, suggesting that TcDRBD3 granules repertoire harbors RNAs released from polysomes. Under starvation, rTcDRBD3 granules localized throughout the cytoplasm and also increased in number in the presence of puromycin. Our results suggest that TcDRBD3 accumulates into perinuclear granules that harbor RNA and also that its localization varies according to the type of stress. (BZ Americas However infection posttranscriptional post transcriptional mechanisms arrest RNAbinding binding proteins bodies centers T DRBD3 DRBD domains Cterminal C terminal lowcomplexity low complexity glutamines TcDRBD rTcDRBD3, rTcDRBD , (rTcDRBD3) exposure Moreover polysomes starvation stress (rTcDRBD3 (rTcDRBD
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