This research aims to develop a precise and efficient dispersive solid-phase microextraction (DSPE) technique using third-generation polyamidoamine (PAMAM) dendrimer-grafted nanoparticles to quantify benzoylurea pesticides in green tea, black tea, and oolong tea infusions. The extraction efficiency is optimized through single-factor adjustments of key parameters, including adsorbent quantity (5-20 mg), adsorption duration (5-20 min), desorbent identity (methanol, acetonitrile), volume (100-600 μL), sodium chloride concentration (0-10%, m/v), and sample solution pH (3-8). The optimized method demonstrates linearity in the range of 2-500 μg L-1, with limits of detection (LODs) ranging from 0.6-1.5 μg L-1 and limits of quantification (LOQs) from 2.0 5.0 μg L-1. Recovery rates of 81.2-96.0% are achieved, with intra-day relative standard deviations (RSDs) of 1.4-2.8% and inter-day relative standard deviations of 2.0-3.9%, indicating high accuracy and repeatability. The successful application of detecting residues in commercial tea samples underscores the strong potential of the technique to determine benzoylurea analyte levels. solidphase solid phase DSPE (DSPE thirdgeneration third generation PAMAM (PAMAM dendrimergrafted dendrimer grafted infusions singlefactor single factor parameters 520 5 20 (5-2 mg, mg , mg) min, min min) methanol, methanol (methanol acetonitrile, acetonitrile acetonitrile) 100600 100 600 (100-60 μL, μL μL) 010%, 010 0 10%, 10 (0-10% m/v, mv m/v m v m/v) 38. 38 3 8 . (3-8) 2500 2 500 2-50 L1, L1 L 1, 1 LODs (LODs 0.61.5 0615 0.6 1.5 6 0.6-1. L- LOQs (LOQs 2. 50 5. L1. 1. 81.296.0% 812960 81.2 96.0% 81 96 81.2-96.0 achieved intraday intra day RSDs (RSDs 1.42.8% 1428 1.4 2.8% 4 1.4-2.8 interday inter 2.03.9%, 2039 3.9%, 9 2.0-3.9% repeatability levels 52 (5- 10060 60 (100-6 010% 01 10% (0-10 (3-8 250 2-5 61 0.61. 061 06 0. 15 0.6-1 296 81.296.0 81296 812 81. 960 96.0 81.2-96. 42 1.42.8 142 14 28 2.8 1.4-2. 03 2.03.9% 203 39 3.9% 2.0-3.9 (5 1006 (100- (0-1 (3- 25 2- 0.61 0.6- 29 81.296. 8129 96. 81.2-96 1.42. 1.4-2 2.03.9 3.9 2.0-3. ( (100 (0- (3 81.296 81.2-9 1.42 1.4- 2.03. 3. 2.0-3 (10 (0 81.29 81.2- 2.03 2.0- (1

Abstract Objective There is an amelioration in mortality rates of septic shock patients with malignancies over time, but it remains uncertain in children. Therefore, the authors endeavored to compare the clinical characteristics, treatment needs, and outcomes of septic shock children with or without malignancies. Methods The authors retrospectively analyzed the data of children admitted to the PICU due to septic shock from January 2015 to December 2022 in a tertiary pediatric hospital. The main outcome was in-hospital mortality. Results A total of 508 patients were enrolled. The proportion of Gram-negative bacteria and fungal infections in children with malignancies was significantly higher than those without malignancies. Septic shock children with malignancies had a longer length of stay (LOS) in the hospital (21 vs. 11 days, p<0.001). However, there were no statistically significant differences in the LOS of PICU (5 vs. 5 days, p = 0.591), in-hospital mortality (43.0 % vs. 49.4 %, p = 0.276), and 28-day mortality (49.2 % vs. 44.7 %, p = 0.452). The 28-day survival analysis (p = 0.314) also showed no significant differences. Conclusion Although there are significant differences in the bacterial spectrum of infections, the septic shock children with or without malignancies showed a similar mortality rate. The septic shock children with malignancies had longer LOS of the hospital. time Therefore characteristics needs 201 202 inhospital 50 enrolled Gramnegative Gram negative (LOS 21 (2 vs 1 days p<0.001. p0001 p<0.001 . 0 001 p<0.001) However ( 0.591, 0591 0.591 , 591 0.591) 43.0 430 43 (43. 494 49 4 49. 0.276, 0276 0.276 276 0.276) 28day day 28 49.2 492 2 (49. 447 44 7 44. 0.452. 0452 0.452 452 0.452) 0.314 0314 314 rate 20 p000 p<0.00 00 059 0.59 59 43. (43 027 0.27 27 (49 045 0.45 45 0.31 031 31 p00 p<0.0 05 0.5 (4 02 0.2 04 0.4 0.3 03 3 p0 p<0. 0. p<0 p<

Resumo O objetivo principal deste estudo é investigar metodologias eficazes e desenvolver técnicas inovadoras para instruir aspirantes a vocalistas pop, focando especificamente no cultivo de atributos vocais para nutrir e ampliar o potencial de jovens intérpretes. No decorrer desta pesquisa foram empregues métodos analíticos, abordagens tipológicas e generalização. A pesquisa produziu insights cruciais sobre os aspectos fundamentais e fatores influentes que contribuem para a adaptação bem-sucedida de jovens intérpretes às demandas do estilo de canto pop. Como resultado, constatou-se a importância das aulas sistemáticas e da prática vocal para o alcance de um alto nível de habilidade vocal. Além disso, o culminar desta investigação científica levou ao desenvolvimento de uma abordagem pedagógica abrangente para o ensino da voz pop. Esta técnica levou em consideração os principais aspectos e fatores que influenciam o sucesso da adaptação de jovens intérpretes ao estilo pop de canto. O método desenvolvido de ensino de vocais pop pode ser usado como base para o desenvolvimento sistemático e passo a passo das habilidades vocais de jovens intérpretes. Os resultados do trabalho obtidos podem ser utilizados para melhorar abordagens de ensino de vocais pop e desenvolver talentos musicais em jovens intérpretes. Esta pesquisa pode servir de base para futuras pesquisas e desenvolvimento no campo do canto pop e da pedagogia musical.

Abstract The primary objective of this study is to investigate effective methodologies and devise innovative techniques for instructing aspiring pop vocalists, specifically focusing on the cultivation of vocal attributes to nurture and amplify the potential of young performers. In the course of this research, analytical methods, typological approaches, and generalization were employed. The research has yielded crucial insights into the fundamental aspects and influential factors that contribute to the successful adaptation of young performers to the demands of the pop singing style. As a result, it was established the importance of systematic classes and vocal practice for achieving a high level of vocal skill. Furthermore, the culmination of this scientific inquiry has led to the development of a comprehensive pedagogical approach for teaching pop vocals. This technique took into account the key aspects and factors influencing the successful adaptation of young performers to the pop style of singing. The developed method of teaching pop vocals can be used as a basis for the systematic and step-by-step development of vocal abilities of young performers. The obtained work results can be used to improve approaches to teaching pop vocals and developing musical talents in young performers. This research can serve as a basis for further research and development in the field of pop singing and music pedagogy.

Resumo: Kong-Zi Jia Yu é um livro que registrou muitas conversas e pensamentos de Confúcio e seus discípulos, tornando-se uma fonte indispensável para explorar a cultura e as ideias tradicionais chinesas. Em particular, as ideias de governança apresentadas no livro ainda são altamente relevantes, hoje. Os pensamentos orientados para as pessoas eram o núcleo da sabedoria da governança, a arte da realeza paradigmática e os cortesãos virtuosos eram uma parte sensata da governança, e as ideias da penologia asseguravam ainda mais suas operações robustas. Na nova era, novas ideias como “colocar as pessoas como prioridade máxima”, os pensamentos de Xi Jinping sobre a construção de equipes de quadros e o governo da China pelo estado de direito foram propostos sucessivamente como conversões criativas e desenvolvimento inovador da cultura chinesa, que foi levado adiante, ao longo de milhares de anos. Herdar e promover esses “genes” culturais, apurar a sua essência ideológica, são de grande valor referencial para o cultivo do panorama político atual, enriquecendo a conotação da construção da equipe de quadros, na nova era, aderindo ao Estado de Direito e dando ideias para a formulação das atuais políticas de governança rural. Resumo KongZi Kong Zi discípulos tornandose tornando se chinesas particular relevantes hoje robustas era colocar máxima, máxima , máxima” chinesa adiante anos genes “genes culturais ideológica atual rural

Abstract: Kong-Zi Jia Yu is a book that recorded many Confucius and his disciples’ conversations and thoughts, making it an indispensable source to explore traditional Chinese culture and ideas. In particular, the governance ideas presented in the book are still highly relevant today. People-oriented thoughts were the core of governance wisdom, paradigmatic kingcraft and virtuous courtiers were a sensible part of governance, and the penological ideas further ensured the robust operations of it. In the new era, new ideas like “putting the people as the top priority”, Xi Jinping’s thoughts on building cadre teams and governing China by the rule of law have successively proposed as creative converts and innovative development of Chinese culture that has been carried forward over thousands of years. Inheriting and promoting these cultural “genes”, refining their ideological essence, are of great reference value for cultivating the current political landscape, enriching the connotation of the construction of the cadre team in the new era, adhering to the rule of law and providing ideas for the formulation of current rural governance policies. Abstract KongZi Kong Zi disciples particular today Peopleoriented People oriented wisdom era putting priority, priority , priority” Jinpings Jinping s years genes, genes “genes” essence landscape policies “genes

The objective of this study was to investigate the effect of seasonal thermal stress on serum hormones, oxidative status, and immune response of periparturient dairy cows. A total of 32 healthy Holstein dairy cows in the perinatal period with the same date of delivery were selected (eight cows per season) and housed in a free-stall barn. Different seasons corresponded to different temperature-humidity indices (THI). Regression analysis revealed a strong positive correlation between THI and rectal temperature and respiratory rate. On the day of calving, the level of progesterone and oestradiol increased and prolactin decreased substantially at a high THI. For oxidative stress biomarkers, the contents of malondialdehyde, superoxide dismutase, and catalase were substantially higher at a low THI. Glutathione peroxidase was increased at a high THI. For immune responses, the data indicated that low and high THI conditions led to an increase in interleukin-2 and interleukin-10. Low THI cows exhibited a substantially higher level of tumour necrosis factor-a before calving. Oxidative stress, inflammatory response, and endocrine imbalance therefore occur in lactating dairy cows in hot summers and cold-wet winters in comparison with comfortable seasons.

Abstract The incidence of non-alcoholic fatty liver (NAFLD) remains high, and many NAFLD patients suffer from severe ischemia-reperfusion injury (IRI). Currently, no practical approach can be used to treat IRI. Puerarin plays a vital role in treating multiple diseases, such as NAFLD, stroke, diabetes, and high blood pressure. However, its role in the IRI of the fatty liver is still unclear. We aimed to explore whether puerarin could protect the fatty liver from IRI. C57BL/6J mice were fed with a high‐fat diet (HFD) followed by ischemia reperfusion injury. We showed that hepatic IRI was more severe in the fatty liver compared with the normal liver, and puerarin could significantly protect the fatty liver against IRI and alleviate oxidative stress. The PI3K-AKT signaling pathway was activated during IRI, while liver steatosis decreased the level of activation. Puerarin significantly protected the fatty liver from IRI by reactivating the PI3K-AKT signaling pathway. However, LY294002, a PI3K-AKT inhibitor, attenuated the protective effect of puerarin. In conclusion, puerarin could significantly protect the fatty liver against IRI by activating the PI3K-AKT signaling pathway. nonalcoholic non alcoholic (NAFLD ischemiareperfusion . (IRI) Currently diseases stroke diabetes pressure However unclear C57BL6J CBLJ C57BL 6J C BL J highfat fat HFD (HFD stress PI3KAKT PIKAKT PI3K AKT PI K activation LY294002 LY inhibitor conclusion (IRI CBL KAKT PIK LY29400 LY2940 LY294 LY29 LY2

SUMMARY Thyroid storm is a rare but well-known life-threatening complication that occurs due to acute exacerbation of thyrotoxicosis with the increased levels of circulating thyroid hormones. Reports of metabolic encephalopathy associated with thyroid storm are scarce. We describe the case of a 23-year-old male patient with no previous history of abnormal thyroid function who had consumed excessive amounts of alcohol before disease onset. The patient was found unconscious and febrile on a roadside by a passerby and was admitted to our hospital's emergency department. His primary clinical presentation included hyperthermia (40.8 °C), nodal tachycardia (180 beats/min), seizures, coma, and hypoglycemia (2.18 mmol/L). The hypoglycemia was quickly corrected after admission, but his level of consciousness showed no improvement. With aggressive screening, the patient was found to have severe thyroid dysfunction (T3 = 6.67 nmol/L, T4 = 252.00 nmol/L, free T3 = 29.20 pmol/L, free T4 = 65.30 pmol/L, and TSH = 0.001 μIU/mL). After medical treatment, plasmapheresis, hemofiltration, and hemoperfusion, the patient showed substantial improvement in thyroid hormone levels and stabilization of vital signs, but the impaired consciousness and seizures persisted. Multiple computed tomography scans revealed brain abnormalities. Magnetic resonance imaging performed after tracheal extubation revealed bilateral frontal lobe lesions. We reported a case of metabolic encephalopathy in a patient with life-threatening thyroid storm and bilateral frontal lobe lesions. Hypoglycemia may have been involved in the development of encephalopathy in our patient. Health care providers should consider thyroid storm in the differential diagnosis of hyperthermia, seizures, and coma. Early plasmapheresis, hemofiltration, and hemoperfusion can lower T4 levels and improve prognosis in patients with thyroid storm and encephalopathy. wellknown well known lifethreatening life threatening hormones scarce 23yearold yearold 23 year old onset hospitals hospital s department 40.8 408 40 8 (40. °C, C °C , °C) 180 (18 beats/min, beatsmin beats/min beats min beats/min) coma 2.18 218 2 18 (2.1 mmol/L. mmolL mmol/L . mmol L mmol/L) admission screening T (T 667 6 67 6.6 nmolL nmol nmol/L 25200 252 00 252.0 2920 29 20 29.2 pmolL pmol pmol/L 6530 65 30 65.3 0001 0 001 0.00 μIU/mL. μIUmL μIU/mL μIU mL μIU/mL) treatment plasmapheresis hemofiltration signs persisted abnormalities lesions 40. 4 (40 (1 2.1 21 1 (2. 66 6. 2520 25 252. 292 29. 653 3 65. 000 0.0 (4 ( 2. (2 0.

Abstract Objective This study aimed to investigate the clinical significance of serum microRNA-146a and pro-inflammatory factors in children with Mycoplasma pneumoniae pneumonia after azithromycin treatment. microRNA-146a is known to regulate inflammatory responses, and excessive inflammation is a primary characteristic of MPP. Methods Children with MPP received conventional symptomatic therapy along with intravenous administration of azithromycin for one week. Serum levels of microRNA-146a and pro-inflammatory factors were measured using RT-qPCR and ELISA kits, respectively. The correlation between microRNA-146a and pro-inflammatory factors was analyzed by the Pearson method. Pulmonary function indexes were assessed using a pulmonary function analyzer, and their correlation with microRNA-146a and pro-inflammatory factors after treatment was evaluated. Children with MPP were divided into effective and ineffective treatment groups, and the clinical significance of microRNA-146a and pro-inflammatory factors was evaluated using receiver operating characteristic curves and logistic multivariate regression analysis. Results Serum microRNA-146a was downregulated in children with MPP but upregulated after azithromycin treatment, contrasting with the trend observed for pro-inflammatory factors. MicroRNA-146a showed a negative correlation with pro-inflammatory cytokines. Pulmonary function parameters were initially reduced in children with MPP, but increased after treatment, showing positive/inverse associations with microRNA-146a and pro-inflammatory factors. Higher microRNA-146a and lower pro-inflammatory factors predicted better efficacy of azithromycin treatment. MicroRNA-146a, tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), interleukin-8 (IL-8), and forced expiratory volume in the first second/forced vital capacity (FEV1/FVC) were identified as independent factors influencing treatment efficacy. Conclusion Azithromycin treatment in children with MPP upregulates microRNA-146a, downregulates pro-inflammatory factors, and effectively improves pulmonary function. microRNA146a microRNAa microRNA 146a proinflammatory pro responses week RTqPCR RT qPCR kits respectively method analyzer groups analysis MicroRNA146a MicroRNAa MicroRNA cytokines positiveinverse positive inverse MicroRNA146a, 146a, factoralpha factor alpha TNFα, TNFα TNF α , (TNF-α) interleukin6 interleukin 6 interleukin- IL6, IL6 IL (IL-6) interleukin8 8 IL8, IL8 (IL-8) secondforced second FEV1/FVC FEV1FVC FEVFVC FEV1 FVC FEV (FEV1/FVC microRNA146a, (TNF-α (IL-6 (IL-8 (IL- (IL

Abstract The osseous vascular endothelium encompasses a vast intricate framework that regulates bone remodeling. Osteoporosis, an age-associated systemic bone disease, is characterized by the degeneration of the vascular architecture. Nevertheless, the precise mechanisms underpinning the metamorphosis of endothelial cells (ECs) with advancing age remain predominantly enigmatic. In this study, we conducted a systematic analysis of differentially expressed genes (DEGs) and the associated pathways in juvenile and mature femoral ECs, utilizing data sourced from the Gene Expression Omnibus (GEO) repositories (GSE148804) and employing bioinformatics tools. Through this approach, we successfully discerned six pivotal genes, namely Adamts1, Adamts2, Adamts4, Adamts14, Col5a1, and Col5a2. Subsequently, we constructed a miRNA-mRNA network based on miRNAs displaying differential expression between CD31hiEMCNhi and CD31lowEMCNlow ECs, utilizing online repositories for prediction. The expression of miR-466i-3p and miR-466i-5p in bone marrow ECs exhibited an inverse correlation with age. Our in vivo experiments additionally unveiled miR-466i-5p as a pivotal regulator in osseous ECs and a promising therapeutic target for age-related osteoporosis. remodeling Osteoporosis ageassociated disease architecture Nevertheless (ECs enigmatic study DEGs (DEGs GEO (GEO GSE148804 GSE (GSE148804 tools approach Adamts1 Adamts Adamts2 Adamts4 Adamts14 Col5a1 Cola Col Col5a2 Subsequently miRNAmRNA miRNA mRNA CDhiEMCNhi CD hiEMCNhi CDlowEMCNlow lowEMCNlow prediction miR466i3p miRip miR 466i 3p i p miR466i5p 5p agerelated related osteoporosis GSE14880 (GSE14880 Col5a GSE1488 (GSE1488 GSE148 (GSE148 GSE14 (GSE14 GSE1 (GSE1 (GSE

ABSTRACT Objectives: To study the complications and effectiveness of the treatment of chronic arrhythmias with cardiac Ganglion Plexus (GP) ablation, and to explore the value of the treatment of chronic arrhythmias with GP ablation. Methods: This study was a one-arm interventional study of patients from the first hospital of Xinjiang Medical University and the People’s Hospital of Xuancheng City admitted (09/2018-08/2021) because of bradyarrhythmia. The left atrium was modeled using the Carto3 mapping system. The ablation endpoint was the absence of a vagal response under anatomically localized and high-frequency stimulation guidance. Postoperative routine follow-up was conducted. Holter data at 3-, 6-, and 12-months were recorded. Results: Fifty patients (25 male, mean age 33.16 ± 7.89 years) were induced vagal response by either LSGP, LIGP, RAGP, or RIGP. The heart rate was stable at 76 bpm, SNRT 1.092s. DC, DR, HR, SDNN, RMSSD values were lower than that before ablation. AC, SSR, TH values were higher than those before ablation, mean heart rate and the slowest heart rate were significantly increased. There were significant differences in follow-up data between the preoperative and postoperative periods (all p < 0.05). All the patients were successfully ablated, and their blood pressure decreased significantly. No complications such as vascular damage, vascular embolism and pericardial effusion occurred. Conclusions: Left Atrial GP ablation has good long-term clinical results and can be used as a treatment option for patients with bradyarrhythmia. Objectives (GP Methods onearm one arm Peoples People s 09/201808/2021 092018082021 09/2018 08/2021 09 2018 08 2021 (09/2018-08/2021 bradyarrhythmia Carto system highfrequency high frequency guidance followup follow up conducted 3, 3 , 3- 6, 6 6- 12months months 12 recorded Results 25 (2 male 3316 33 16 33.1 789 7 89 7.8 years LSGP LIGP RAGP RIGP bpm 1092s 1 092s 1.092s DC DR HR SDNN AC SSR increased all 0.05. 005 0.05 . 0 05 0.05) ablated damage occurred Conclusions longterm long term 201808 09/201808/202 09201808202 092018 09/201 082021 08/202 201 202 (09/2018-08/202 2 ( 331 33. 78 8 7. 00 0.0 20180 09/201808/20 0920180820 09201 09/20 08202 08/20 20 (09/2018-08/20 0. 09/201808/2 092018082 0920 09/2 0820 08/2 (09/2018-08/2 09/201808/ 09201808 092 09/ 082 08/ (09/2018-08/ 09/201808 0920180 (09/2018-08 09/20180 (09/2018-0 (09/2018- (09/2018 (09/201 (09/20 (09/2 (09/ (09 (0

ABSTRACT Objective: This study aimed to identify differentially expressed microRNAs (miRNAs) in exosomes derived from the blood plasma of Rheumatoid Arthritis (RA) patients and explore their clinical significance and biological roles. Methods: Illumina high-throughput sequencing was employed to measure miRNA expression levels in plasma exosomes, followed by validation using qRT-PCR. The correlation between exosomal miRNAs and disease activity was systematically analyzed. Additionally, the pathogenic effects of RA exosomes were investigated through bioinformatics analysis and in vitro experiments. Results: Significantly reduced levels of exosomal miR-144-3p and miR-30b-5p were observed in RA patients, which were negatively correlated with DAS28 scores and anti-CCP antibody levels. ROC curve analysis showed that miR-144-3p and miR-30b-5p in plasma exosomes could effectively distinguish RA patients from healthy controls, with AUC values of 0.725 and 0.773, respectively. Combining bioinformatics analysis and in vitro experiments, it was demonstrated that plasma exosomes contribute to ongoing autoantibody production in RA by promoting B-cell differentiation and antibody production. Conclusion: The present study indicates that plasma exosomes from RA patients may be potentially pathogenic. Exosomal miR-144-3p and miR-30b-5p exhibit significant decreases in RA patients and are associated with disease activity, suggesting their potential as valuable biomarkers for RA. Objective (miRNAs (RA roles Methods highthroughput high throughput qRTPCR. qRTPCR qRT PCR. PCR qRT-PCR analyzed Additionally experiments Results miR1443p miRp miR 144 3p p miR30b5p miRbp 30b 5p b DAS DAS2 antiCCP anti CCP controls 0725 0 725 0.72 0773 773 0.773 respectively Bcell B cell Conclusion 14 072 72 0.7 077 77 0.77 1 07 7 0.

Abstract Objective: To explore the relationship between Anion Gap (AG), Albumin Corrected AG (ACAG), and in-hospital mortality of Acute Myocardial Infarction (AMI) patients and develop a prediction model for predicting the mortality in AMI patients. Methods: This was a retrospective cohort study based on the Medical Information Mart for Intensive Care (MIMIC)-III, MIMIC-IV, and eICU Collaborative Study Database (eICU). A total of 9767 AMI patients who were admitted to the intensive care unit were included. The authors employed univariate and multivariable cox proportional hazards analyses to investigate the association between AG, ACAG, and in-hospital mortality; p < 0.05 was considered statistically significant. A nomogram incorporating ACAG and clinical indicators was developed and validated for predicting mortality among AMI patients. Results: Both ACAG and AG exhibited a significant association with an elevated risk of in-hospital mortality in AMI patients. The C-index of ACAG (C-index = 0.606) was significantly higher than AG (C-index = 0.589). A nomo-gram (ACAG combined model) was developed to predict the in-hospital mortality for AMI patients. The nomo-gram demonstrated a good predictive performance by Area Under the Curve (AUC) of 0.763 in the training set, 0.744 and 0.681 in the external validation cohort. The C-index of the nomogram was 0.759 in the training set, 0.756 and 0.762 in the validation cohorts. Additionally, the C-index of the nomogram was obviously higher than the ACAG and age shock index in three databases. Conclusion: ACAG was related to in-hospital mortality among AMI patients. The authors developed a nomogram incorporating ACAG and clinical indicators, demonstrating good performance for predicting in-hospital mortality of AMI patients. Objective , (AG) (ACAG) inhospital hospital (AMI Methods MIMICIII, MIMICIII MIMIC III, III (MIMIC)-III MIMICIV, MIMICIV IV, IV MIMIC-IV eICU. . (eICU) 976 included 005 0 05 0.0 Results Cindex C 0.606 0606 606 0.589. 0589 0.589 589 0.589) nomo gram AUC (AUC 0763 763 0.76 set 0744 744 0.74 0681 681 0.68 0759 759 0.75 0756 756 0762 762 cohorts Additionally databases Conclusion (AG (eICU 97 00 0. 0.60 060 60 058 0.58 58 076 76 0.7 074 74 068 68 0.6 075 75 9 06 6 0.5 5 07 7

ABSTRACT Objective: The purpose of the present study was to examine the association of oxidative stress markers with sarcopenia in the general United States population under the age of 60. Methods: We used the National Health and Nutrition Examination Survey data from 2011‒2014 and performed Restricted Cubic Spline (RCS) plots, weighted multivariable logistic regression analysis to calculate ratio ratios and 95% Confidence Intervals, and subgroup analysis based on age, sex, hypertension, diabetes mellitus, and body mass index stratification to determine the association of markers of oxidative stress with the prevalence of sarcopenia. Results: The present analysis included a total of 8,782 participants. Firstly, the RCS plots showed a roughly L-shaped curve association of total bilirubin and serum iron with a prevalence of sarcopenia. Secondly, albumin was negatively and linearly associated with the risk of sarcopenia. Finally, with the increase in gamma-glutamyl transferase, the prevalence of sarcopenia showed a trend of first rising and then declining as a result of the iron increase. Conclusions: We demonstrated a nonlinear association between markers of oxidative stress and sarcopenia. The need to focus more on levels of oxidative stress in the body could provide better prevention strategies for sarcopenia. Objective 60 Methods 20112014 2011 2014 2011‒201 (RCS 95 Intervals sex hypertension mellitus Results 8782 8 782 8,78 participants Firstly Lshaped L shaped Secondly Finally gammaglutamyl gamma glutamyl transferase Conclusions 6 2011201 201 2011‒20 9 878 78 8,7 201120 20 2011‒2 87 7 8, 20112 2 2011‒

Abstract Objective To investigate the reasons for a second surgery after Percutaneous Endoscopic Surgery (PES) for lumbar spinal stenosis and to provide references for the choice of indications and appropriate surgical approach. Method A total of 426 patients received PES for lumbar spinal stenosis. The postoperative, intraoperative, and postoperative data of the subjects were analyzed. The reasons for intraoperative difficulties, poor outcomes after surgery, and a second surgery were analyzed. Result The surgical approach was changed in four out of 426 patients (0.94 %) during surgery, and 6 patients (1.4 %) received a second surgery; 3 out of 4 patients were intraoperatively shifted to PIED using the Delta endoscope, and 1 shifted to ordinary PIED. The reasons for the intraoperative change of surgical approach included severe hyperplasia and obscure anatomic structure in 3 patients and a dural sac tear with neural outflow in 1 patient. The reasons for a second surgery in 19 patients were as follows: nerve entrapment by bone fragments in 1 patient; nerve injury in 3 patients; lumbar instability in 3 patients; untreated or residual Lumbar Intervertebral Disc Herniation (LIDH) in 4 patients; recurrent LIDH in 1 patient; and inadequate decompression in 7 patients. Conclusion Severe hyperplasia, obscure anatomic structure, lumbar instability, and nerve injury are the common reasons for a second surgery after PES for lumbar spinal stenosis. Appropriate indications and surgical approaches can be chosen based on the patient's situations and technical conditions. (PES 42 analyzed difficulties 0.94 094 0 94 (0.9 % 1.4 14 (1. endoscope patient follows (LIDH s conditions 0.9 09 9 (0. 1. (1 0. (0 (