Abstract Primary ciliary dyskinesia (PCD) is a rare genetic disease characterized by an abnormality in the function or structure of the cilia, coursing with pulmonary, otological and nasosinusal manifestations. Hypoacusis affects 60% of patients with PCD and results mainly from otitis media with effusion (OME) and otological complications such as simple or cholesteatomatous chronic otitis media (COM). Clinical case: Female patient, 52 years old, with PCD, followed in the Otorhinolaryngology department for bilateral COM and chronic rhinosinusitis. Her surgical history includes nasosinusal endoscopic surgery and repeated myringotomies with placement of transtympanic ventilation tubes (TVTT), bilaterally. Despite the placement of TVTT, the simple tone audiogram revealed bilateral severe chronic mixed deafness, with mean bone thresholds of 36.25 dB on the right and 30 dB on the left. Ear tomography excluded erosion or discontinuity of ossicular chains, presence of cholesteatoma and pathology of the inner ear. The patient underwent surgery to place a bone conduction implant in her right ear. After 4 weeks, the processor was activated. The free field audiogram revealed a mean threshold of 26.25dB. Conclusion: In PCD patients, insertion of TVTT is still a controversial treatment for OME. Persistent postoperative otorrhea is a frequent side effect and may complicate the use and tolerance of hearing aids. Bone conduction implants should be considered in auditory rehabilitation, with excellent results.

Resumo A discinésia ciliar primária (DCP) é uma doença genética rara caracterizada por uma anormalidade da função ou da estrutura dos cílios, cursando com manifestações pulmonares, otológicas e nasossinusais. A hipoacusia afeta 60% dos pacientes com DCP e resulta sobretudo de otite média com efusão (OME) e de complicações otológicas, como otite média crónica (OMC) simples ou colesteatomatosa. Caso clínico: Doente do sexo feminino, 52 anos, portadora de DCP, seguida na consulta de Otorrinolaringologia por OMC bilateral e rinossinusite crónica. Tem como antecedentes cirúrgicos, cirurgia endoscópica nasossinusal e repetidas miringotomias com colocação de tubos de ventilação transtimpânicos (TVTT), bilateralmente. Apesar da colocação de TVTT, o audiograma tonal simples revelava uma surdez mista severa crónica bilateralmente, com limiares ósseos médios de 36,25 dB à direita e de 30 dB à esquerda. A tomografia computorizada dos ouvidos excluiu erosão ou descontinuidade das cadeias ossiculares, presença de colesteatoma e patologia do ouvido interno. A doente foi submetida a cirurgia para colocação de implante de condução óssea no ouvido direito. Após 4 semanas da cirurgia, foi feita a ativação do processador. O audiograma tonal simples, em campo livre, revelou um limiar aéreo médio de 26,25dB. Conclusão: Nos doentes com DCP, a inserção de TVTT é ainda um tratamento controverso da OME. A otorreia pós-operatória persistente é um efeito colateral frequente, podendo complicar o uso e tolerância dos aparelhos auditivos. Por este motivo deverão ser considerados os implantes de condução óssea na reabilitação auditiva, apresentando excelentes resultados.

Abstract Despite presenting several advantages in relation to the mortar coating performed by hand labor traditionally carried out in Brazil, there is little knowledge available about mortar pumping systems. Thus, selecting a mortar pump system is not a trivial procedure, and there are no significative studies published in the specialized literature to fully understand the decision criteria applying to this equipment selection. Therefore, the present study aimed to identify the relevant criteria and quantify their importance in the selection of this equipment, by developing a framework combining both mortar pump specialists expertise and fuzzy analytic hierarchy process method to aid this decision making process. Results showed that acquisition cost and quality are the most relevant criteria, representing togheter around 2/3 of the decision weight, while technical and operational aspects account for aproximatelly 1/10 of relevance in the equipment selection. Brazil systems Thus procedure Therefore 23 2 3 2/ weight 110 1 10 1/1 11 1/

ABSTRACT Objective: Previous studies indicated that face masks reduce the probability of infection by SARS-CoV-2 but did not examine the relationship between SARS-CoV-2 viral load and mask usage. This study analyzed this relationship. Methods: This cross-sectional study evaluated patients admitted to a public Emergency Care Unit in Belo Horizonte, MG, Brazil, between October 2020 and March 2021. Convenience samples were recruited during the study period. Adult patients with COVID-19 symptoms were invited to participate after being examined by a physician according to the Emergency Care Unit routine. This study included 441 adults with mild symptoms diagnosed with COVID-19 via RT-PCR, who were tested until the 14th day after symptom onset. Participants were interviewed about mask usage, oropharyngeal swabs were collected, and the SARS-CoV-2 viral load was measured using RT-PCR. The difference between the SARS-CoV-2 viral load in the groups of individuals who regularly used and did not use masks was subjected to a hypothesis test. Results: Of the patients who had swabs collected 1-5 days after symptom onset, 64.9% of those not using masks regularly and 45.5% of those using masks regularly presented a high SARS-CoV-2 viral load. This difference was statistically significant (p=0.0073). Considering only the patients who had swabs collected 4-8 days after symptom onset, 44.1% of those not using masks regularly and 30.8% of those using masks regularly presented a high SARS-CoV-2 viral load; this difference was statistically significant (p=0.0364). Conclusion: When people who regularly wear a face mask contract COVID-19, they have a significantly lower probability of having a high viral load of SARS-CoV-2 compared to those who do not regularly wear masks. Objective SARSCoV2 SARSCoV SARS CoV 2 SARS-CoV- usage Methods crosssectional cross sectional Horizonte MG Brazil 202 2021 period COVID19 COVID 19 COVID-1 routine 44 RTPCR, RTPCR RT PCR, PCR RT-PCR th onset RTPCR. PCR. test Results 15 1 5 1- 649 64 9 64.9 455 45 45.5 p=0.0073. p00073 p p=0.0073 . 0 0073 (p=0.0073) 48 4 8 4- 44.1 308 30 30.8 p=0.0364. p00364 p=0.0364 0364 (p=0.0364) Conclusion COVID19, 19, SARS-CoV 20 COVID1 COVID- 6 64. 45. p0007 p=0.007 007 (p=0.0073 44. 3 30. p0036 p=0.036 036 (p=0.0364 p000 p=0.00 00 (p=0.007 p003 p=0.03 03 (p=0.036 p00 p=0.0 (p=0.00 (p=0.03 p0 p=0. (p=0.0 p=0 (p=0. p= (p=0 (p= (p

BACKGROUND Anopheles darlingi is the most efficient vector of malaria parasites in the Neotropics. Nevertheless, the specificities of its larval habitats are still poorly known. OBJECTIVES Characterize permanent larval habitats, and population dynamics of An. darlingi and other potential vectors in relation to climate, physicochemical variables, insect fauna and malaria cases. METHODS A 14-month longitudinal study was conducted in Porto Velho, Rondônia, western Brazilian Amazon. Monthly, 21 permanent water bodies were sampled. Immature anophelines and associated fauna were collected, physicochemical characteristics, and climate variables were recorded and analyzed. FINDINGS Five types of habitats were identified: lagoon, stream, stream combined with lagoon, stream combined with dam, and fishpond. A total of 60,927 anophelines were collected. The most abundant species in all habitats were Anopheles braziliensis and An. darlingi. The highest density was found in the lagoon, while streams had the highest species richness. Abundance was higher during the transition period wet-dry season. There was a lag of respectively four and five months between the peak of rainfall and the Madeira River level and the highest abundance of An. darlingi larvae, which were positively correlated with habitats partially shaded, pH close to neutrality, increase dissolved oxygen and sulphates. MAIN CONCLUSIONS The present study provides data on key factors defining permanent larval habitats for the surveillance of An. darlingi and other potential vectors as well as a log-linear Negative Binomial model based on immature mosquito abundance and climate variables to predict the increase in the number of malaria cases. Neotropics Nevertheless known An cases 14month month 14 Velho Rondônia Amazon Monthly 2 sampled collected characteristics analyzed identified lagoon dam fishpond 60927 60 927 60,92 richness wetdry wet dry season larvae shaded neutrality sulphates loglinear log linear 1 6092 6 92 60,9 609 9 60,

RESUMO O objetivo deste trabalho foi avaliar a caracterização físico-química e adsortiva do rejeito de Fe coletado no distrito de Brumadinho; e verificar seu efeito na germinação de Raphanus sativus. O material foi coletado na camada superficial (0–20 cm) e desintegrado para determinação de pH, potencial redox (Eh), condutividade elétrica (EC), matéria orgânica (MO), capacidade de troca catiônica (CEC), área superficial específica (SSA) e de grupos funcionais. Estudos de adsorção foram conduzidos utilizando azul de metileno (MB). Os resultados dos estudos de adsorção foram analisados utilizando modelos cinéticos (Elovich, pseudoprimeira ordem – PFO e pseudossegunda ordem – PSO) e modelos isotérmicos (Freundlich, Langmuir e Sips). O rejeito apresenta pH ácido (5,60), ΔpH negativo (-0,30) e baixa CEC (1,85 cmolc g-1). Foi verificada alta eficiência de adsorção de MB (96%). O modelo de Elovich (0,9248 < R2 < 0,9858) representou melhor a cinética química e o modelo de Freundlich descreveu melhor o processo de adsorção de MB no rejeito (R2 = 0,9609). A capacidade máxima de adsorção (qm) foi igual a 15,08 mg g-1. A presença de rejeito de Fe influenciou positivamente a germinação de sementes de R. sativus (73,8%), mas o crescimento do caule e da raiz foi inferior quando comparado às mudas cultivadas em substrato composto. Conclui-se que o material tem capacidade de adsorção catiônica favorável, o que pode beneficiar a fertilização do solo. No entanto, o desenvolvimento de R. sativus foi menor no substrato de rejeito de Fe, provavelmente devido à baixa CTC, MO e disponibilidade de nutrientes. físicoquímica físico Brumadinho 0–20 020 0 20 (0–2 cm Eh, Eh , (Eh) EC, EC (EC) MO, (MO) CEC, (CEC) SSA (SSA funcionais MB. . (MB) Elovich, (Elovich PSO Freundlich, (Freundlich Sips. Sips Sips) 5,60, 560 5,60 5 60 (5,60) 0,30 030 30 (-0,30 1,85 185 1 85 (1,8 g1. g1 g g-1) 96%. 96 96% (96%) 0,9248 09248 9248 (0,924 R 0,9858 09858 9858 (R 0,9609. 09609 0,9609 9609 0,9609) qm (qm 1508 15 08 15,0 1. g-1 73,8%, 738 73,8% 73 8 (73,8%) composto Concluise Conclui se favorável solo entanto CTC nutrientes 0–2 02 2 (0– (Eh (EC (MO (CEC (MB 56 5,6 6 (5,60 0,3 03 3 (-0,3 1,8 18 (1, 9 (96% 0,924 0924 924 (0,92 0,985 0985 985 0960 0,960 960 150 15, g- 73,8 7 (73,8% 0– (0 5, (5,6 0, (-0, 1, (1 (96 0,92 092 92 (0,9 0,98 098 98 096 0,96 73, (73,8 ( (5, (-0 (9 0,9 09 (0, (73, (5 (- (73 (7

ABSTRACT The objective of this work was to evaluate the physicochemical and adsorptive characterization of Fe tailing collected in the district of Brumadinho; and verify its effect on Raphanus sativus germination. The material was collected on the surface layer (0-20 cm) and disintegrated for pH, redox potential – Eh, electrical conductivity – EC, OM, cation exchange capacity – CEC, specific surface area – SSA and functional groups characterization. Adsorption studies were conducted using methylene blue (MB). The results of the adsorption studies were analyzed using kinetic models (Elovich, pseudo-first order – PFO and pseudo-second order – PSO) and isotherm models (Freundlich, Langmuir and Sips). The tailing has an acidic pH (5.60), negative ΔpH (-0.30) and low CEC (1.85 cmolc g-1). A high MB adsorption efficiency (96%) was verified. The Elovich model (0.9248<R2<0.9858) best represented the chemical kinetics, and the Freundlich model best describes the MB adsorption process in the tailing (R2 = 0.9609). The maximum adsorption capacity (qm) was equal to 15.08 mg g-1. The presence of Fe tailing positively influenced the germination of R. sativus seeds (73.8%), but stem and root growth were inferior when compared to seedlings cultivated in compost substrate. It is concluded that the material has favorable cationic adsorption capacity, which can benefit soil fertilization. However, R. sativus development was minor in Fe tailing substrate, probably due to low CEC, OM and nutrient availability. Brumadinho 020 0 20 (0-2 cm Eh EC MB. . (MB) Elovich, (Elovich pseudofirst pseudo first pseudosecond second PSO Freundlich, (Freundlich Sips. Sips Sips) 5.60, 560 5.60 , 5 60 (5.60) 0.30 030 30 (-0.30 1.85 185 1 85 (1.8 g1. g1 g g-1) 96% 96 (96% verified 0.9248<R2<0.9858 09248R209858 R 9248 R2 9858 (0.9248<R2<0.9858 kinetics (R 0.9609. 09609 0.9609 9609 0.9609) qm (qm 1508 15 08 15.0 1. g-1 73.8%, 738 73.8% 73 8 (73.8%) substrate fertilization However availability 02 2 (0- (MB 56 5.6 6 (5.60 0.3 03 3 (-0.3 1.8 18 (1. 9 (96 0.9248<R2<0.985 09248R20985 924 985 (0.9248<R2<0.985 0960 0.960 960 150 15. g- 73.8 7 (73.8% (0 5. (5.6 0. (-0. (1 (9 0.9248<R2<0.98 09248R2098 92 98 (0.9248<R2<0.98 096 0.96 73. (73.8 ( (5. (-0 0.9248<R2<0.9 09248R209 (0.9248<R2<0.9 09 0.9 (73. (5 (- 0.9248<R2<0. 09248R20 (0.9248<R2<0. (73 0.9248<R2<0 09248R2 (0.9248<R2<0 (7 0.9248<R2< 09248R (0.9248<R2< 0.9248<R2 (0.9248<R2 0.9248<R (0.9248<R

Abstract Vaccines are considered one of the most important public health advancements of the modern age and the most cost-effective preventive method of curbing the spread of several diseases with high morbidity and lethality. However, vaccination coverage rates around the world have been threatened by the vaccine hesitancy phenomenon, considered by the World Health Organization (WHO) as one of the ten greatest menaces to public health in the world. Vaccine opposition is a mentality driven by various interests, as old as the vaccine itself, predominantly using the media and social networks, which have an enormous influence on people. They often spread false information about vaccines, frequently with no adequate scientific backing, negatively impacting vaccination rates. To understand the possible correlation between the information spread on social media and childhood vaccination rates, we performed a narrative review on the subject, with a qualitative approach, in original or review articles, indexed in national and international databases and/or journals, in Portuguese and English, published between 2011 and 2021, available in full and free of charge. One hundred and seventy-two articles were extracted from the literature, 49 of which were submitted to analysis. The use of content analysis in a narrative literature review made it possible to interpret the main publications that in the last decade point to the impact of social media as one of the main factors linked to vaccine hesitancy in children. The articles show that communication is presented as a determinant for adherence or vaccine hesitancy, and social media are a useful tool for disseminating information that can influence and mobilize the population, promoting its acceptance or having a negative impact on childhood vaccine coverage rates. Social Media has become fertile ground for the creation and spread of fake news, hence the importance of the health professional's engagement in strengthening the population's confidence in relation to immunization, minimizing vaccine hesitancy.

Abstract In the community, malnutrition is manifested by the loss of muscle mass, being associated with a decrease in muscle strength, an increased risk of frailty, functional impairment and impaired healing of chronic wounds, as well as a higher incidence of infections. It is estimated that malnutrition affects one in three elderly people at home and one in three patients in elderly care institutions. Malnutrition is reversible as long as the patient has access to timely nutritional screening that identifies their nutritional risk and as long as an individualized nutritional intervention is instituted. Disease associated malnutrition continues to be perceived as a secondary problem in primary health care, so the implementation of nutritional risk screening, as a routine parameter, is the first step in improving access to nutritional care in the community. In this article, the Malnutrition Universal Screening Tool is proposed for routine nutritional screening in primary care.

Resumo Na comunidade, a malnutrição manifesta-se pela perda de massa muscular, estando associada à diminuição da força muscular, ao aumento do risco de fragilidade, ao comprometimento funcional e da cicatrização de feridas crónicas, assim como a maior incidência de infeções. Estima-se que a malnutrição afete um em cada três idosos no seu domicílio e um em cada três utentes em instituições de cuidados de idosos. A malnutrição é reversível desde que o utente tenha acesso a um atempado rastreio nutricional que identifique o seu risco nutricional e instituída uma intervenção nutricional individualizada. A malnutrição associada à doença continua a ser percecionada como um problema secundário nos cuidados de saúde primários, pelo que a implementação do rastreio do risco nutricional, como um parâmetro de rotina, é o primeiro passo na melhoria do acesso aos cuidados nutricionais na comunidade. Neste artigo, é proposta a ferramenta Malnutrition Universal Screening Tool para rastreio nutricional de rotina nos cuidados de saúde primários.

Resumo As coleções e pesquisas feitas nas primeiras décadas do século XX, no Rio de Janeiro, foram fundamentais para o estudo da sistemática e da história natural dos mosquitos no Brasil. Um personagem de destaque nesse cenário foi Antonio Gonçalves Peryassú. Analisamos o histórico de uma coleção por ele organizada no Museu Nacional do Rio de Janeiro, entre 1918 e 1922. XX Janeiro Brasil Peryassú 191 1922 19 192 1

Abstract Collections formed and studies conducted in the early decades of the twentieth century in Rio de Janeiro, Brazil, were crucial for the study of the systematization and natural history of mosquitoes in Brazil. One key figure in this context was Antonio Gonçalves Peryassú. The history of a collection he organized at Museu Nacional [National Museum] in Rio de Janeiro between 1918 and 1922 is analyzed. Brazil Peryassú National Museum 191 192 analyzed 19 1

ABSTRACT This study proposes a strategy for large-scale testing among a large number of people for the early diagnosis of COVID-19 to elucidate the epidemiological situation. Pool testing involves the analysis of pooled samples. This study aimed to discuss a reverse transcription technique followed by quantitative real-time polymerase chain reaction using pool testing to detect SARS-CoV-2 in nasopharyngeal swab samples. The study proposes an innovative diagnostic strategy that contributes to resource optimization, cost reduction, and improved agility of feedback from results. largescale scale COVID19 COVID 19 COVID-1 situation samples realtime real time SARSCoV2 SARSCoV SARS CoV 2 SARS-CoV- optimization reduction results COVID1 1 COVID- SARS-CoV

ABSTRACT Public Science and Technology Institutions (STIs) need to assume and consolidate a proactive role in innovation management to transform knowledge advances into appropriate results for the industrial base. It is required a strategic managerial action aimed at sustaining the sector’s ecosystem and guided by the principle of entrepreneurship. Considering the context of STIs in an integrated approach to the technological innovation process, two questions were raised: How to guarantee an architecture and organizational governance that favor entrepreneurial behavior by linking it to the technological innovation strategy in the ecosystem, and how to expand and promote interactions among ecosystem agents, ensuring an environment leading to innovation and the dissemination of the knowledge generated. The proposed managerial model can be applied in any national or international technology-based innovation ecosystem keeping the characteristics of the business environment. The authors used extensive literature research and direct observation to develop ideas and link them to this management practice. The result was a strategic innovation management model for technology-based STIs. It has management instruments that enhance the proactive performance of ecosystem agents and helps them transfer and absorb the developed technologies. (STIs base sectors sector s entrepreneurship process raised generated technologybased technology based practice technologies

ABSTRACT Insects are fundamental to biodiversity conservation in almost all ecosystems, and their population decline, and extinction directly result from environmental impacts. These facts are aggravated by the lack of knowledge of insect biodiversity, the so-called biodiversity deficits, especially the Linnean and Wallacean shortfalls. In freshwater ecosystems, biodiversity loss is higher among aquatic insects, and caddisflies comprise one of the most vulnerable orders. In this way, research focusing on describing new caddisfly species and understanding their distribution ranges will increase knowledge of caddisfly biodiversity. In the past 14 years, the team from the Laboratório de Entomologia Aquática “Prof. Dr. Claudio Gilberto Froehlich” (LEAq, Universidade Federal da Bahia, Brazil) and collaborators have been addressing these issues, resulting in 55 described species of caddisflies. Taxa in other insect orders have also received attention and an additional 16 species have been described (eight mayflies, four stoneflies, four neuropteran spongillaflies, and antlions). Here, eight caddisfly species are described and illustrated (Atopsyche froehlichi sp. nov., Austrotinodes zeferina sp. nov., Cernotina kariri sp. nov., Neoathripsodes froehlichi sp. nov., Notalina claudiofroehlichi sp. nov., Oecetis marcus sp. nov., Phylloicus froehlichi sp. nov., and Polycentropus claudioi sp. nov.), five of them in honor of LEAq’s patron. In addition, new distributional data are presented for 10 known species. A checklist of the caddisfly fauna of Bahia state is also presented, with 138 species, around 75% and 30% of them recorded and described by the LEAq team, respectively. Currently, for caddisflies, Bahia is the fifth most species-rich state in Brazil, and the first in the Brazilian Northeast region. ecosystems decline impacts socalled so called deficits shortfalls insects way 1 years Prof. Prof “Prof Dr Froehlich LEAq, (LEAq Brazil issues 5 mayflies stoneflies spongillaflies antlions. antlions . antlions) Here Atopsyche sp nov nov. , nov.) LEAqs s patron addition 13 75 30 respectively Currently speciesrich rich region 7 3

Resumo A Sociedade Brasileira de Angiologia e Cirurgia Vascular, por meio do projeto Diretrizes, apresenta as novas Diretrizes de Aorta Abdominal, referentes aos cuidados de pacientes com aneurisma de aorta abdominal. Para sua elaboração, foram priorizadas diretrizes descritivas, utilizando as bases EMBASE, LILACS e PubMed. As referências incluem ensaios clínicos randomizados, revisões sistemáticas, metanálises e estudos de coorte. A qualidade das evidências foi examinada por uma dupla de coordenadores, com auxílio da ferramenta RoB 2 da Colaboração Cochrane e dos formulários da Newcastle Ottawa Scale. Aneurismas justarrenais, infectados e novas técnicas terapêuticas, principalmente no âmbito endovascular, estão entre os temas estudados. A versão atual das Diretrizes apresenta importantes recomendações para os principais itens que envolvem o diagnóstico, tratamento e acompanhamento de pacientes com aneurisma de aorta abdominal, oferecendo um guia objetivo para prática médica, construído a partir de evidências científicas e amplamente acessível em todo o território nacional. Vascular Abdominal abdominal elaboração descritivas EMBASE PubMed randomizados sistemáticas coorte coordenadores Scale justarrenais terapêuticas endovascular estudados diagnóstico médica nacional

Abstract The Brazilian Society of Angiology and Vascular Surgery, through the Guidelines Project, presents new Abdominal Aortic Aneurysm Guidelines, on the subject of care for abdominal aortic aneurysm patients. Its development prioritized descriptive guidelines, using the EMBASE, LILACS, and PubMed databases. References include randomized controlled trials, systematic reviews, meta-analyses, and cohort studies. Quality of evidence was evaluated by a pair of coordinators, aided by the RoB 2 Cochrane tool and the Newcastle Ottawa Scale forms. The subjects include juxtarenal aneurysms, infected aneurysms, and new therapeutic techniques, especially endovascular procedures. The current version of the guidelines include important recommendations for the primary topics involving diagnosis, treatment, and follow-up for abdominal aortic aneurysm patients, providing an objective guide for medical practice, based on scientific evidence and widely available throughout Brazil. Surgery Project patients EMBASE LILACS databases trials reviews metaanalyses, metaanalyses meta analyses, analyses meta-analyses studies coordinators forms aneurysms techniques procedures diagnosis treatment followup follow up practice Brazil

Abstract Objective: To evaluate physical and mental health indicators in adolescents with preexisting chronic immunocompromised conditions during coronavirus disease 2019 (COVID-19) quarantine. Methods: A cross-sectional study included 355 adolescents with chronic conditions and 111 healthy adolescents. An online self-rated survey was used to investigate socio-demographic features, healthcare routine, and the quarantine impact on physical and mental health. The validated self-reported version of the Strengths and Difficulties Questionnaire (SDQ) was also applied. Results: The median of age [14 (10–18) vs. 15 (10–18) years, p = 0.733] and frequencies of female (61% vs. 60%, p = 0.970) were similar between adolescents with preexisting chronic conditions and healthy adolescents during quarantine of COVID-19 pandemic. The frequencies of abnormal total difficulties score of SDQ were similar in patients and controls (30% vs. 31%, p = 0.775). Logistic regression analysis showed that being female (OR = 1.965; 95% CI = 1.091–3.541, p = 0.024), fear of underlying disease activity/complication (OR = 1.009; 95%CI = 1.001–1.018, p = 0.030) were associated with severe psychosocial dysfunction in adolescents with chronic conditions, whereas school homework (OR = 0.449; 95% CI = 0.206–0.981, p = 0.045) and physical activity (OR = 0.990; 95% CI = 0.981–0.999, p = 0.030) were protective factors. Further analysis of patients with chronic immunocompromised conditions and previous diagnosis of mental disorders (9%) compared with patients without diagnosis showed higher median of total difficulties score (p = 0.001), emotional (p = 0.005), conduct (p = 0.007), peer problems (p = 0.001) and hyperactivity (p = 0.034) in the former group. Conclusion: Adolescents with preexisting chronic immunocompromised conditions during COVID-19 quarantine were not at higher risk of adverse health indicators. Being female, fear of underlying disease activity/complication, and household members working outside of the home were relevant issues for adolescents with preexisting chronic conditions. This study reinforces the need to establish mental health strategies for teens with chronic conditions, particularly during the pandemic.

Objective: To show the feasibility of using combined nasopharyngeal swab auto-collection and pool testing to detect SARS-CoV-2 in epidemiological surveys. Methods: The study involved a sample of 154 students from the Universidade Federal de Minas Gerais, who performed the self-collection of the nasopharyngeal swab in individual booths without supervision. Molecular testing was performed using the pool testing technique. Results: Obtaining samples lasted about 5 minutes each. Analysis 6 was performed to detect endogenous RNA in 40 samples, and the results indicated that no failures resulted from self-collection. None of the pools detected the presence of viral RNA. The cost of performing the molecular test (RT-PCR) by pool testing with samples obtained by self-collection was about 10 times lower than with the usual methods. Conclusion: The investigated strategies showed to be economically feasible and valid for the research of SARS-CoV-2 in epidemiological surveys.

Objetivo: Demostrar la viabilidad de utilizar el uso combinado de la autocollección de swabs nasofaríngeos y pool testing para la detección del SARS-CoV2 en encuestas epidemiológicas. Métodos: El estudio involucró a una muestra de 154 estudiantes de la Universidade Federal de Minas Gerais, quienes realizaron la autocolección del hisopo nasofaríngeo en cabinas individuales sin supervision. La prueba molecular se realizó utilizando la técnica de prueba de grupo. Resultados: La obtención de muestras duró unos 5 minutos por persona. Se realizó un análisis para detectar RNA endógeno en 40 muestras y los resultados indicaron que no hubo fallas derivadas de la autocolección. Ninguno de los grupos detectó la presencia de RNA viral. El costo de realizar una prueba molecular (RT-PCR) por pool con muestras obtenidas por auto-recolección fue aproximadamente 10 veces menor que con los métodos habituales. Conclusión: Las estrategias investigadas demonstraram ser económicamente viables y válidas para la investigación del SARS-CoV-2 en encuestas epidemiológicas.

Objetivo: Demonstrar a viabilidade da utilização combinada da autocoleta de swab nasofaríngeo e pool testing para detecção do SARS-CoV-2 em inquéritos epidemiológicos. Métodos: O estudo envolveu amostra de 154 estudantes da Universidade Federal de Minas Gerais, que realizaram a autocoleta do swab nasofaríngeo em cabines individuais e sem supervisão. O teste molecular foi realizado utilizando-se a técnica de pool testing. Resultados: A obtenção de amostras durou cerca de 5 minutos por pessoa. Realizou-se análise para detecção de RNA endógeno em 40 amostras e os resultados indicaram que não houve falhas decorrentes da autocoleta. Nenhum dos pools detectou presença de RNA viral. O custo da realização do teste molecular (RT-PCR) por pool testing com amostras obtidas por autocoleta foi cerca de dez vezes menor do que nos métodos habituais. Conclusão: As estratégias investigadas mostraram-se economicamente viáveis e válidas para a pesquisa de SARS-CoV-2 em inquéritos epidemiológicos.

ABSTRACT Background: Haiti is one of the Caribbean countries where malaria persists. More than 99% of malaria cases are caused by Plasmodium falciparum, the main vector being the mosquito Anopheles albimanus. In this paper, we describe the epidemiological profile of malaria in Haiti between 2009 and 2018. Methods We analyzed information on cases reported by the Ministry of Health of Haiti and the World Health Organization (WHO). Results: Between 2009 and 2018, 232,479 malaria cases were reported by the Ministry of Public Health and Population (MSPP); an increase in the incidence of malaria in the country in 2010, followed by a decrease in 2011, was primarily observed. Due to recent efforts to reduce malaria by 2020, its incidence declined from 60,130 cases in 2010 to 8,978 cases in 2018. Controversially, in terms of the number of reported cases, the MSPP and WHO report conflicting data. However, the results from both datasets present the same trend in Haiti from 2009 to 2018. The results also illustrate the endemicity of the disease throughout Haiti, both in rural and urban areas, especially along the coast. Conclusions: This study emphasizes the need to promote official data collection and analyses, as well as the application of epidemiological surveillance of malaria at the municipal level, for a better understanding of the real impact of malaria on the Haitian population and to create more appropriate interventions.