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au:Lima, Wagner C.

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DESENVOLVIMENTO DE MATRIZ DE SÍLICA AMINOFUNCIONALIZADA COMO POTENCIAL ADSORVENTE DE ÍONS Pt4+ EM SISTEMAS DE PRÉ-CONCENTRAÇÃO PARA ANÁLISE POR FAAS Pt4 Pt PRÉCONCENTRAÇÃO PRÉ CONCENTRAÇÃO
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Nascimento, Caio V. L. V. do

; Araújo, Arthur L. S. de

; Lima, Lidiane M. A. de

; Sousa, Filipe C. P. de

; Silva, Wagner E.

; Lavorante, André F.

; Belian, Mônica F.

Química Nova

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2025, Volume 48 N. 1 elocation e-20250008

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

The use of materials such as organofunctionalized silica for application in solid phase extraction (SPE) allows the preconcentration of several ions, including Pt, and subsequent detection. This work aims to synthesize and characterize an organofunctionalized silica (Sil-TMSDT) with the silylant N’(trimethoxysilyl)propylethylenetriamine and its application as a solid phase in the preconcentration Pt4+ ions in aqueous media, using detection by flame atomization atomic absorption spectroscopy (FAAS). The Fourier-transform infrared spectroscopy (FTIR) spectra and scanning electron microscopy (SEM) coupled with energy-dispersive spectroscopy (EDS) confirmed the aminofunctionalization of the silica. For the adsorption process, a preconcentration mini-column with 200 mg of Sil-TMSDT, a solution of 0.485 mg L−1 of K2[PtCl6] at pH 10 and HNO3 0.5 mol L−1 as eluent were used, obtaining an enrichment factor of 15-fold with retention efficiency higher than 99%. The Pt4+ ion determined by FAAS showed a linear response between 0.02 and 0.40 mg L−1; a linear coefficient where R = 0.9981; a 16-fold preconcentration factor; a limit of quantification (LOQ) and limit of detection (LOD) of 0.045 and 0.014 mg L−1, respectively. The proposal system showed analytical applicability to determining Pt4+ ion in an aqueous medium and could be used to detect traces of this potentially toxic metal in biological fluids such as urine and blood. SPE (SPE Pt SilTMSDT Sil TMSDT (Sil-TMSDT Ntrimethoxysilylpropylethylenetriamine N trimethoxysilyl propylethylenetriamine Pt4 media FAAS. . (FAAS) Fouriertransform Fourier transform FTIR (FTIR SEM (SEM energydispersive energy dispersive EDS (EDS process minicolumn mini column 20 SilTMSDT, TMSDT, Sil-TMSDT 0485 0 485 0.48 L1 L 1 L− K2PtCl6 KPtCl K2 PtCl6 K PtCl K2[PtCl6 HNO 05 5 0. 15fold fold 15 99 99% 002 02 0.0 040 40 0.4 0.9981 09981 9981 16fold 16 LOQ (LOQ LOD (LOD 0045 045 0.04 0014 014 0.01 respectively blood (FAAS 2 048 48 K2PtCl K2[PtCl 9 00 04 4 0.998 0998 998 004 001 01 0.99 099 0.9 09

https://doi.org/10.21577/0100-4042.20250008

Obituary: Nelio Roberto dos Reis (1950-2023) Obituary 19502023 1950 2023 (1950-2023 1950202 195 202 (1950-202 195020 19 20 (1950-20 19502 1 2 (1950-2 (1950- (1950 (195 (19 (1 (
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Peracchi, Adriano L.

; Pereira, Alan D.

; Batista, Carolina B.

; Cheida, Carolina C.

; Lima, Isaac P. de

; Sekiama, Margareth L.

; Rocha, Vlamir J.

; Pedro, Wagner A.

Zoologia (Curitiba)

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2024, Volume 41 elocation e24013

Abstract: En | Text: En | PDF: En

https://doi.org/10.1590/s1984-4689.v41.e24013

Antarctic environments as a source of bacterial and fungal therapeutic enzymes
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LIMA, IGOR G.O.

; BISPO, JAMES R.S.

; AGOSTINHO, ADSON Y.H.

; QUEIROZ, ALINE C. DE

; MOREIRA, MAGNA SUZANA A.

; PASSARINI, MICHEL RODRIGO Z.

; OLIVEIRA, VALÉRIA M. DE

; SETTE, LARA D.

; ROSA, LUIZ HENRIQUE

; DUARTE, ALYSSON WAGNER F.

Anais da Academia Brasileira de Ciências

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2022, Volume 94 Suppl. 1 elocation e20210452

Abstract: En | Text: En | PDF: En

Abstract Microbial therapeutic enzymes are the protagonists in the pharmacological treatment of different human diseases. The intrinsic enzymatic characteristics, such as high affinity and specificity to the corresponding substrate, enable effective therapies, with minimal adverse effects and complete remission. However, immunogenicity, short half-life, low enzymatic yield, and low selectivity regarding available enzyme drugs are currently the main obstacles to their development and the broad adherence to therapeutic protocols. By harboring adapted and still unexplored microbial life, environments of extreme conditions, such as Antarctica, become especially important in the prospecting and development of new enzymatic compounds that present higher yields and the possibility of genetic improvement. Antarctic microorganisms have adaptation mechanisms, such as more fluid cell membranes, production of antifreeze proteins and enzymes with more malleable structures, more robust, stable, selective catalytic sites for their respective substrates, and high antioxidant capacity. In this context, this review aims to explore enzymes synthesized by bacteria and fungi from Antarctica as potential drug producers, capable of providing therapeutic efficacy, less adverse effects, and lower production costs with highlight to L-Asparaginase, collagenase, superoxide dismutase and ribonucleases. In addition, this review highlights the unique biotechnological profile of these Antarctic extremophile microorganisms.

https://doi.org/10.1590/0001-3765202220210452

COMVC-19: A Program to protect healthcare workers’ mental health during the COVID-19 Pandemic. What we have learned
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Fukuti, Pedro

; Uchôa, Caroline Louise Mesquita

; Mazzoco, Marina Flaborea

; Cruz, Isabella D'Andrea Garcia da

; Echegaray, Mariana V.F.

; Humes, Eduardo de Castro

; Silveira, Júlia Belizário

; Santi, Talita Di

; Miguel, Euripedes Constantino

; Corchs, Felipe

; Fatori, Daniel

; Campello, Guilherme

; Oliveira, Gabriel M. de

; Argolo, Felipe C.

; Ferreira, Felipe de M.

; Machado, Gustavo

; Argeu, Adriana

; Oliveira, Graça Maria Ramos de

; Serafim, Antônio de Pádua

; Siqueira, Luciana de Lima

; Rossi, Luciane de

; Rios, Izabel Cristina

; Oliveira, Talita Rodrigues de

; Antoniazzi, Leilane C. K.

; Gagliotti, Daniel Augusto Mori

; Abelama Neto, Emílio

; Oliveira Junior, Paulo Novais de

; Correia, Aline Villalobo

; Gonçalves, Luca Schilling

; Tortato, Liana Silva

; Busato, Wagner Machado Moraes

; Guimarães-Fernandes, Flávio

; Alves, Marcos

; Leite Netto, Oswaldo Ferreira

; Schoueri, Patrícia de Campos Lindenberg

; Roque, Márcio de Assis

; Merlin, Silvia Stahl

; Boer, Giovana Cardoso Machado

; Sallet, Paulo Clemente

; Malbergier, André

; Spedo, Mariana Abrahão

; Kamitsuji, Carla Satie

; Faria, Elizabeth de

; Moreira, Moacyr Vergara de Godoy

; Kaufman, Arthur

; Abdo, Carmita

; Scanavino, Marco de Tubino

; Lancman, Selma

; Tavares, Hermano

; Polanczyk, Guilherme

; Brunoni, André R.

; Forlenza, Orestes V.

; Barros-Filho, Tarcísio Eloy Pessoa de

Clinics

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2021, Volume 76 elocation e2631

Abstract: En | Text: En | PDF: En

OBJECTIVE: In 2020, the COVID-19 pandemic brought a work and stress overload to healthcare workers, increasing their vulnerability to mental health impairments. In response, the authors created the COMVC-19 program. The program offered preventive actions and mental health treatment for the 22,000 workers of The Hospital das Clinicas da Faculdade de Medicina da Universidade de São Paulo (HCFMUSP). This paper aims to describe its implementation and share what we have learned from this experience. METHODS: Workers were able to easily access the program through a 24/7 hotline. Additionally, a mobile phone app that screened for signs and symptoms of emotional distress and offered psychoeducation and/or referral to treatment was made available. Data from both these sources as well as any subsequent psychiatric evaluations were collected. RESULTS: The first 20 weeks of our project revealed that most participants were female, and part of the nursing staff working directly with COVID-19 patients. The most frequently reported symptoms were: anxiety, depression and sleep disturbances. The most common diagnoses were Adjustment, Anxiety, and Mood disorders. CONCLUSIONS: Implementing a mental health program in a multimodal intervention was feasible in a major quaternary public hospital. Our data also suggests that preventive actions should primarily be aimed at anxiety and depression symptoms, with a particular focus on the nursing staff.

https://doi.org/10.6061/clinics/2021/e2631

Experimental infection by Anaplasma marginale in buffaloes and cattle: clinical, hematological, molecular and pathological aspects
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Lima, Danillo H.S.

; Vinhote, Wagner M.S.

; Ubiali, Daniel G.

; Soares, Pierre C.

; Cordeiro, Matheus D.

; Silva, Jenevaldo B.

; Fonseca, Adivaldo H.

; Barbosa, José D.

Pesquisa Veterinária Brasileira

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sep 2019, Volume 39 N. 9 Pages 700 - 709

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO: O estudo teve como objetivo avaliar e comparar os aspectos clínicos, laboratoriais e patológicos de búfalos e bovinos infectados experimentalmente com estirpe AmRio 2 de Anaplasma marginale. Para isso, foram utilizados quatro bubalinos Murrah e quatro bovinos mestiços, sendo dois animais de cada espécie, esplenectomizados. Estirpe AmRio 2 de A. marginale foi inoculada em todos os animais. Foram realizados exames clínicos, hematócrito, hemograma, esfregaço sanguíneo com avaliação de riquetsemia, necropsia e histopatologia, além de, Semi-Nested-PCR (snPCR) para o gene alvo msp5 e snPCR para o gene alvo msp1α para identificação de A. marginale nas amostras de sangue dos ruminantes. A partir das amostras positivas na snPCR msp1α, foram selecionadas amostras para análise das sequências de aminoácidos deste gene. Dois bovinos esplenectomizados apresentaram apatia, mucosas pálidas, icterícia, hipertermia e anemia severa. O restante dos animais não apresentou sintomatologia clínica. A riquetsemia em todos os animais foi menor que 1%. A média do hematócrito dos bovinos esplenectomizados esteve abaixo de 20% em dois momentos após infecção. Ao hemograma, as principais alterações observadas foram nos bovinos esplenectomizados e caracterizaram-se por redução de hemácias, hemoglobina, hematócrito e plaquetas (p<0,05). Todos os animais apresentaram elevação de leucócitos por aumento de linfócitos, porém, sem diferença significativa. O período pré-patente médio foi de dois dias em todos os animais. O período de incubação médio nos bovinos que adoeceram foi de 25,5 dias e estes morreram em média 63 dias após inoculação da estirpe. Os achados de necropsia caracterizaram-se por carcaça pálida, ascite, aumento de volume do fígado, vesícula biliar distendida e bile espessa. À histopatologia, verificou-se infiltração de macrófagos e linfócitos em diversos órgãos, dilatação dos sinusoides hepáticos e necrose do intestino grosso. A snPCR para o gene msp5, revelou 100% dos animais positivos em pelo menos um momento de avaliação. E na snPCR para o gene alvo msp1α também verificou-se infecção em todos os animais em pelo menos uma amostra avaliada. Entretanto, o sequenciamento revelou apenas cinco animais, incluindo os bovinos que morreram, com similaridade das sequências de aminoácidos com estirpe AmRio 2 de A. marginale. Conclui-se que os bovinos esplenectomizados morreram em virtude de anaplasmose provocada pela estirpe inoculada e os bubalinos foram mais resistentes em comparação aos bovinos. Finalmente, os búfalos podem ser uma alternativa à criação de bovinos em áreas com alta ocorrência de casos clínicos de anaplasmose.

ABSTRACT: The study aimed to evaluate and compare the clinical, laboratory and pathological aspects of buffalo and bovine experimentally infected with AmRio 2 strain of Anaplasma marginale. Four Murrah buffaloes and four crossbred cattle were used in the experiment, which two animals of each species were splenectomized. Strain AmRio 2 of A. marginale was inoculated in all experimental animals. Clinical exams, Packed Cell Volume (PCV), blood counts, blood smears, rickettsemia, necropsy and histopathology were performed in all cases. Semi-Nested-PCR (snPCR) for the msp5 and snPCR for the msp1α target gene for identification of A. marginale in blood samples from animals was done. From positive samples for msp1α snPCR, samples were analyzed for the amino acid sequences of this gene. Two splenectomized cattle presented apathy, pale mucous membranes, jaundice, hyperthermia, and severe anemia. The remaining experimental animals did not show clinical signs. The rickettsemia in all animals was less than 1%. The mean PCV of the splenectomized cattle was below 20% at two-time points after infection. On the blood count, the main changes were observed in splenectomized calves and were characterized by a decrease in red blood cells, hemoglobin, PCV and platelets (p <0.05). All animals presented leukocyte elevation by increased lymphocytes, however, with no significant difference. The average prepatent period was two days in all the animals. The average incubation period in cattle that became ill was 25.5 days, and death occurred, on average, 63 days after inoculation of the strain. The necropsy findings were characterized by pale carcass, ascites, enlarged liver, distended gallbladder, and thick bile. Histopathological findings included infiltration of macrophages and lymphocytes in various organs, hepatic sinusoidal dilatation, and necrosis of the large intestine. In snPCR for the msp5 gene, 100% of the animals were positive in at least one evaluation. And in the snPCR for the infection of the msp1α target gene was also found in all animals in at least one sample evaluated. However, sequencing revealed only five animals, including the bovine which died, with a similarity of the amino acid sequences with AmRio 2 strain of A. marginale. It is concluded that the splenectomized cattle died due to anaplasmosis caused by the inoculated strain and the buffalo were more resistant compared to cattle. Buffaloes can be an alternative to cattle rearing in areas with a high occurrence of clinical cases of anaplasmosis.

https://doi.org/10.1590/1678-5150-pvb-6273 1883 downloads

Obesity Resistance Promotes Mild Contractile Dysfunction Associated with Intracellular Ca2+ Handling
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Sá, Felipe Gonçalves dos Santos de

; Lima-Leopoldo, Ana Paula

; Jacobsen, Bruno Barcellos

; Ferron, Artur Junio Togneri

; Estevam, Wagner Muller

; Campos, Dijon Henrique Salomé

; Castardeli, Edson

; Cunha, Márcia Regina Holanda da

; Cicogna, Antonio Carlos

; Leopoldo, André Soares

Arquivos Brasileiros de Cardiologia

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dec 2015, Volume 105 N. 6 Pages 588 - 596

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Resumo Fundamento: A obesidade induzida por dieta é frequentemente utilizada para demonstração de disfunção cardíaca. No entanto, alguns ratos, como humanos, são suscetíveis ao desenvolvimento de um fenótipo de obesidade, enquanto outros são resistentes. Objetivo: Avaliar a relação entre resistência à obesidade e função cardíaca e o impacto da resistência à obesidade no trânsito de cálcio. Métodos: Ratos Wistar machos com trinta dias de idade foram distribuídos em dois grupos com 54 animais cada: controle (C; dieta padrão) e obesos (quatro dietas palatáveis hiperlipídicas) por 15 semanas. Após o protocolo experimental, os ratos alimentados por dietas hiperlipídicas foram classificados de acordo com o índice de adiposidade e subdivididos em propensos à obesidade (PO) e resistentes à obesidade (RO). Foram avaliados o perfil nutricional, comorbidades e remodelação cardíaca. A função cardíaca foi avaliada pelo estudo do músculo papilar isolado em condições basais e após manobras inotrópicas. Resultados: As dietas hiperlipídicas promoveram aumento na gordura corporal e no índice de adiposidade em ratos PO comparados com ratos C e RO. Os perfis de glicose, lipídios e pressão arterial permaneceram inalterados em ratos RO. Além disso, os ratos RO apresentaram peso total do coração e dos ventrículos direito e esquerdo mais baixos do que ratos PO, mas semelhantes aos ratos C. Os músculos cardíacos de todos os ratos apresentaram dados semelhantes na condição basal, mas a resposta miocárdica a um estímulo de contração pós-pausa estava comprometida em ratos PO e RO em comparação aos ratos C. Conclusão: A resistência à obesidade promoveu alterações específicas na fase de contração, sem alterar a fase de relaxamento. Esta anormalidade leve pode estar relacionada com o trânsito intracelular de Ca+2.

Abstract Background: Diet-induced obesity is frequently used to demonstrate cardiac dysfunction. However, some rats, like humans, are susceptible to developing an obesity phenotype, whereas others are resistant to that. Objective: To evaluate the association between obesity resistance and cardiac function, and the impact of obesity resistance on calcium handling. Methods: Thirty-day-old male Wistar rats were distributed into two groups, each with 54 animals: control (C; standard diet) and obese (four palatable high-fat diets) for 15 weeks. After the experimental protocol, rats consuming the high-fat diets were classified according to the adiposity index and subdivided into obesity-prone (OP) and obesity-resistant (OR). Nutritional profile, comorbidities, and cardiac remodeling were evaluated. Cardiac function was assessed by papillary muscle evaluation at baseline and after inotropic maneuvers. Results: The high-fat diets promoted increase in body fat and adiposity index in OP rats compared with C and OR rats. Glucose, lipid, and blood pressure profiles remained unchanged in OR rats. In addition, the total heart weight and the weight of the left and right ventricles in OR rats were lower than those in OP rats, but similar to those in C rats. Baseline cardiac muscle data were similar in all rats, but myocardial responsiveness to a post-rest contraction stimulus was compromised in OP and OR rats compared with C rats. Conclusion: Obesity resistance promoted specific changes in the contraction phase without changes in the relaxation phase. This mild abnormality may be related to intracellular Ca2+ handling.

https://doi.org/10.5935/abc.20150134 2025 downloads

Aluminum stress in Crambe abyssinica Hochst
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Colodetti, Tafarel Victor

; Nunes Rodrigues, Wagner

; Deleon Martins, Lima

; Tomaz, Marcelo Antonio

Idesia (Arica)

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may 2015, Volume 33 N. 2 Pages 31 - 39

Abstract: En Es | Text: En Es | PDF: En | PDF: Es

Crambe is a sensitive specie to exchangeable aluminum, it require a well-fertilized and corrected soil. The objective of this study was to understand the impact of aluminum presence, in different concentrations, in the substrate on the germination and development of crambe. The experiment was carried out as a randomized complete block design with a 5x2 factorial arrangement in four replicates. The five concentrations of Al3+ were 0, 15, 40, 80 and 120 mg dm-3 Al3+ and two temperatures (20 and 30 °C), with four replications. The relative growth rate, plant height, leaf number, total leaf area, specific leaf area, dry matter in each plant compartment (root, stem and leaf), leaf area ratio, stem mass ratio and leaf mass ratio were evaluate. Germination was favored by 20 ºC. The limitation on plant growth was greater as the concentration of aluminum increased, presenting losses in growth, as well of production of dry matter and leaf development, also causing changes in the patterns of biomass allocation between the plant compartments.

Crambe es una especie sensible al aluminio intercambiable que requiere suelos bien fertilizados y corregidos. El objetivo de este trabajo fue evaluar distintas concentraciones de aluminio en la germinación y desarrollo de crambe. El experimento para germinación se realizó con un diseño de bloques completos al azar con arreglo factorial de 5x2, con cuatro repeticiones. Las cinco concentraciones de Al3+ fueron 0, 15, 40, 80 y 120 mg dm-3 y las temperaturas de 20 y 30° C. El experimento para desarrollo de la planta se realizó con un diseño de bloques completos al azar con cinco concentraciones de Al3+ y cuatro repeticiones. Los parámetros evaluados fueron porcentaje de germinación, crecimiento de las plántulas y biomasa. Se analizó la tasa relativa de crecimiento, altura de planta, número de hojas, área foliar total, área foliar específica, materia seca en cada compartimento de la planta (raíz, tallo y hojas), razón de área foliar, razón de masa de raíz, tallo y hojas. El porcentaje de germinación aumentó a los 2 °C. La mayor concentración de aluminio afectó el crecimiento, producción de materia seca y desarrollo de hojas. Estos resultados sugieren que el cramble es sensible al aluminio intercambiable.

1005 downloads

In vitro propagation of Cyrtopodium saintlegerianum rchb. f. (orchidaceae), a native orchid of the Brazilian savannah
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Rodrigues, Lennis Afraire

; Paiva Neto, Vespasiano Borges de

; Boaretto, Amanda Galdi

; Oliveira, Janaína Fernanda de

; Torrezan, Mateus de Aguiar

; Lima, Sebastião Ferreira de

; Otoni, Wagner Campos

Crop Breeding and Applied Biotechnology

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mar 2015, Volume 15 N. 1 Pages 10 - 17

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Visando à obtenção de grande quantidade de mudas para programas de reintrodução, bem como viabilizar sua exploração comercial, sementes de Cyrtopodium saintlegerianum foram incubadas em meio Knudson. Após a germinação, os protocormos foram inoculados em meio Knudson suplementado com 6-benzilaminopurina (BA) e ácido α-naftalenoacético (ANA) para indução de brotações, que foram posteriormente individualizadas e inoculadas em meio Knudson acrescido com ácido giberélico (AG3), para promover alongamento. As plantas resultantes do processo in vitro foram avaliadas e transplantadas para bandejas contendo Plantmax(r) e casca de Acurí triturada. A relação auxina citocinina influencia na propagação in vitro de C. saintlegerianum, induzindo um incremento no número de brotações quando o meio de cultura foi acrescido de 2,0 mg L-1 de BA, na ausência ou presença de 0,5 mg L-1 de ANA. A espécie demonstrou-se promissora na multiplicação massal in vitro. O uso do ácido giberélico favoreceu o alongamento das brotações in vitro, mas contribuiu negativamente na aclimatização das plantas, tornando desnecessária a utilização deste fitorregulador na produção de mudas de C. saintlegerianum.

In order to enable production of large quantities of plantlets for reintroduction programs, as well as economic exploration, Cyrtopodium saintlegerianum seeds were sown on Knudson culture medium. After seed germination, the protocorms were inoculated on Knudson culture medium supplemented with 6-benzyladenine (BA) and α-naphthaleneacetic acid (NAA). The obtained shoots were individually inoculated in Knudson supplemented with gibberellic acid (GA3) in order to promote elongation. Seedlings were evaluated and then transplanted into trays containing commercial substrate Plantmax(r)-HT, or crushed Acuri leaf sheath. Auxin/cytokinin ratio influenced in vitro propagation of C. saintlegerianum, resulting in increased shoot number when 2.0 mg L-1 BA was added to the culture medium in the absence or presence of 0.5 mg L-1 NAA. This species proved to be promising for massal in vitro multiplication. Despite having incremented in vitro shoots elongation, the use of GA3 is unnecessary since it contributed negatively in the acclimatization of plants.

https://doi.org/10.1590/1984-70332015v15n1a2 2653 downloads

Celiotomy by plastrotomy in a yellow-footed tortoise (Geochelone denticulata)
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Rodrigues, Marcelo C.

; Lima, Wagner C.

; Quessada, Ana M.

; Silva, Filipi A.N.

; Silva, Laís M.C.

; Souza, André B. de

; Moura, Charlys R.C. de

; Lima, Dayanne A.S.D.

Pesquisa Veterinária Brasileira

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feb 2015, Volume 35 N. 2 Pages 173 - 176

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Neste relato descreve-se um caso bem sucedido de celiotomia por plastrotomia para remoção de corpos estranhos em uma jabuti-tinga (Geochelone denticulata). O animal foi atendido no Hospital Veterinário da Universidade Federal do Piauí apresentando falta de apetite, regurgitação, constipação, letargia, relutância em andar e mucosas oculares hiperêmicas. Foi realizado exame radiográfico, o qual confirmou presença de corpos estranhos no estômago. O animal foi submetido à celiotomia por plastrotomia para remoção dos corpos estranhos. A abertura do plastrão foi realizada através dos escudos abdominais, com o auxílio de uma mini-serra circular de retífica. Foi feita uma incisão na linha média entre as duas veias abdominais para se acessar a cavidade abdominal e, uma vez exposto, o estômago foi incisado para remoção dos corpos estranhos (grampos, palitos de dente, pedras, peças de plástico, fragmentos de louças e vidros). A cirurgia foi bem sucedida e a remoção total dos corpos estranhos foi confirmada por radiografia realizada no pós-operatório imediato. A celiotomia por plastrotomia para remoção de corpos estranhos neste animal demonstrou ser uma técnica muito importante, segura e viável para a sobrevivência de quelônios.

One herein reports a successful case of celiotomy by plastrotomy for removal of foreign bodies in yellow-footed tortoise (Geochelone denticulata). The animal was treated at the Veterinary Hospital of the Federal University of Piaui, with appetite loss, regurgitation, constipation, lethargy, reluctance to walk and slightly reddish ocular mucous membranes. Radiographic examination was performed, confirming the presence of foreign bodies in the stomach. The tortoise underwent celiotomy by plastrotomy for the removal of the foreign bodies. The opening of the plastron was performed through the abdominal shields, with the aid of a circular mini grinding saw. One performed an incision in the midline between the two abdominal veins to access the abdominal cavity. A gastrotomy for removal of the foreign bodies (nails, toothpicks, stones, pieces of plastic, glass and crockery pieces) was performed after the location of the stomach. The surgery was successful and confirmed with radiographic evaluation in the immediate postoperative period. The celiotomy by plastrotomy for removal of foreign bodies in that animal proved to be a viable, very important and safe technique to the survival of chelonians.

https://doi.org/10.1590/S0100-736X2015000200014 3249 downloads

10.

Controle pós-colheita da antracnose do maracujazeiro: amarelo com aplicação de óleo de copaíba
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Araújo Neto, Sebastião Elviro de

; Francisco, Wagner de Moura

; Andrade Neto, Romeu de Carvalho

; Lustosa, Camila

; Ribeiro, Sandra Albuquerque Lima

Revista Brasileira de Fruticultura

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jun 2014, Volume 36 N. 2 Pages 509 - 514

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

O objetivo deste trabalho foi avaliar a aplicação de óleo essencial de copaíba no controle da antracnose, nos frutos do maracujazeiro-amarelo, e comparar sua ação fungicida/fungistática in vitro com o óleo resina de copaíba. No experimento in vivo, os frutos foram inoculados com uma suspensão de esporos da ordem de 10(6) conídios mL-1 e 1% de Tween 80, acondicionados em bandejas de polipropileno e colocados em câmara incubadora com temperatura de 25ºC e 90% de umidade relativa do ar. Passadas 24 horas da inoculação, pulverizou-se óleo essencial nas seguintes concentrações: T1= 0 mL L-1; T2= 0,25 mL L-1; T3= 0,5 mL L-1; T4= 0,75 mL L-1; T5= 1,0 mL L-1, sendo avaliados a perda de massa do fruto, a severidade da antracnose e o número de lesões, ambas aos seis dias. Para o experimento in vitro, utilizou-se do meio de cultura batata-dextrose-ágar (BDA) que, após ser esterilizado em autoclave (120 ºC), recebeu óleo essencial e óleo resina de copaíba (0; 0,5; 1,0; 1,5 e 2,0 mL L-1). Após o resfriamento do meio de cultura, foi repicado para o centro da placa um disco de micélio de 12,5 mm de diâmetro de Colletotrichum gloeosporioides; e as placas, incubadas a 25ºC e 90% de umidade. A aferição do crescimento micelial foi verificada com o auxílio de paquímetro analógico, após sete dias de crescimento micelial. O óleo essencial de copaíba, nas concentrações de 0,25 mL L-1 a 1.0 mL L-1, não foi eficaz no controle pós-colheita do fungo da antracnose in vivo e na perda de massa dos frutos de maracujá. O óleo resina de copaíba inibiu o crescimento de C. gloeosporioides in vitro de forma mais eficiente que o óleo essencial de copaíba.

The objective of this study was to evaluate application of the essential oil of copaiba in controlling anthracnose in fruits of passion fruit and compare their fungicidal/fungistatic action in vitro with copaiba oleoresin. In vivo experiment, fruits were inoculated with a spore suspension of 10(6) conidia mL-1 and 1% Tween 80, packed in polypropylene trays and placed in a dry incubator at temperature of 25 ºC and 90% of relative humidity. After 24 hours of inoculation, essential oil was sprayed at the following concentrations: T1 = 0 mL L-1, T2 = 0.25 mLL-1, T3 = 0.5 ml L-1, T4 = 0.75 mLL-1, T5 = 1.0 mLL-1, and evaluated the mass loss of the fruit, anthracnose severity and number of lesions, both at six days. For the in vitro study, we used culture media potato dextrose agar (PDA) which, after being sterilized by autoclaving (120 ºC), was added copaiba oleoresin and essential oil (0, 0.5, 1.0 , 1.5 and 2.0 mL L-1). After cooling the PDA culture media was peaked to the center of the plate a mycelial disc of 12.5 mm diameter of Colletotrichum gloeosporioides and the plates incubated at 25 ºC and 90% humidity. The measurement of mycelial growth was measured with the aid of analog caliper, after seven days of mycelial growth. The copaiba essential oil, in concentrations of 0.25 mL L-1 up to 1.0 mL L-1, was not effective in controlling post-harvest of the pathogen in vivo and in mass loss of passion fruits. The copaiba oils inhibited the growth of C. gloeosporioides in vitro more efficiently than the copaiba essential oil.

https://doi.org/10.1590/0100-2945-146/13 2907 downloads

11.

Low-intensity resistance exercise does not affect cardiac autonomic modulation in patients with peripheral artery disease
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Andrade Lima, Aluísio H.R.

; Farah, Breno Quintella

; Rodrigues, Lausanne B.C.C.

; Miranda, Alessandra S.

; Rodrigues, Sérgio L.C.

; de A. Correia, Marilia

; Sobral Filho, Dario C.

; Forjaz, Cláudia L.M.

; Prado, Wagner Luiz

; Wolosker, Nelson

; Ritti-Dias, Raphael M.

Clinics

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may 2013, Volume 68 N. 5 Pages 632 - 637

Abstract: En | Text: En | PDF: En

OBJECTIVE: To analyze the effect of a single bout of resistance exercise on cardiac autonomic modulation in patients with peripheral artery disease. METHODS: Fifteen patients with peripheral artery disease (age: 58.3±4.0 years) underwent the following sessions in a random order: resistance exercise (three sets of 10 repetitions of the six resistance exercises with a workload of 5-7 in the OMNI-RES scale) and control (similar to the resistance session; however, the resistance exercises were performed with no load). The frequency domain (low frequency, high frequency and sympathovagal balance) and symbolic analysis (0V, 1V and 2V patterns) of heart rate variability were obtained before and until one hour after the interventions. RESULTS: After the resistance exercise and control sessions, similar increases were observed in the consecutive heartbeat intervals (control: 720.8±28.6 vs. 790.9±34.4 ms; resistance exercise: 712.9±30.1 vs. 756.8±37.9 ms; p<0.05) and in the pattern of the symbolic analysis with no variation (0V) (control: 25.1±3.5 vs. 33.4±4.1%; resistance exercise: 26.1±3.2 vs. 29.7±3.5%; p<0.05) until 50 min after both interventions. The pattern of two variations (2V) decreased similarly (control: 11.2±2.1 vs. 8.3±2.1%; resistance exercise: 9.5±1.7 vs. 7.8±1.7%; p<0.05). In contrast, the pattern of one variation (1V), the low and high frequency bands and sympathovagal balance did not change after the interventions (p>0.05). CONCLUSION: A single bout of resistance exercise did not alter cardiac autonomic modulation in patients with peripheral artery disease.

https://doi.org/10.6061/clinics/2013(05)09 1973 downloads Cited 1 time in SciELO

12.

Influence of pre-germination treatments and temperature on the germination of crambe seeds (Crambe abyssinica Hochst)
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Deleon Martins, Lima

; Pianna Costa, Felipe

; Carlos Lopes, José

; Nunes Rodrigues, Wagner

Idesia (Arica)

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dec 2012, Volume 30 N. 3 Pages 23 - 28

Abstract: En Pt | Text: En Pt | PDF: En | PDF: Pt

The objective of this study was to evaluate the influence of pre-germination treatments and temperature on the germination of crambe seed (Crambe abyssinica Hochst). The completely randomized design was used in a 3 x 3 x 2 factorial (physical treatments x chemical treatments x temperature). The chemical treatments consisted of paper soaked in gibberellic acid (GA3), potassium nitrate (KNO3) and water. The physical treatments consisted of maintaining the seed intact, mechanical scarification and seed coat removal. Temperatures of 25 and 30 °C were used in the completely randomized design. The variables studied were germination speed index, germination percentage, seed length and seedling dry mass. In the studied conditions, the percentage and rate of germination, length and seedling dry weight of seeds of C. abyssinica Hochst were enhanced by removal of the integument, in soaking with GA3 at 25 °C.

Objetivou-se com o presente trabalho, avaliar a influência de tratamentos pré-germinativos e de temperaturas na germinação de sementes de crambe (Crambe abyssinica Hochst), em esquema fatorial 3 x 3 x 2 (tratamentos químicos x tratamentos físicos x temperatura). Sendo os tratamentos químicos: ácido giberélico (GA3), nitrato de potássio (KNO3) e água. E os tratamentos físicos: semente intacta, escarificação mecânica e a retirada do tegumento. Foram utilizadas as temperaturas de 25 e 30 °C em delineamento inteiramente casualizado. Estudou-se as seguintes variáveis: índice de velocidade e porcentagem de germinação, comprimento e massa seca das plántulas. Nas condições de estudo, a porcentagem e o índice de velocidade de germinação, o comprimento e a massa seca de plántulas das sementes de C. abyssinica Hochstforam incrementados com a remoção do tegumento, sob o umedecimento com GA3 na temperatura de 25 °C.

1793 downloads Cited 3 times in SciELO

13.

Misturas em tanque com glyphosate para o controle de trapoeraba, erva-de-touro e capim-carrapicho em soja RR®
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Maciel, Cleber Daniel de Goes

; Poletine, Juliana Parisotto

; Amstalden, Saulo Leme

; Gazziero, Dionísio Luis Piza

; Raimondi, Michel Alex

; Lima, Gesley Ramos Guimarães

; Oliveira Neto, Antônio Mendes de

; Guerra, Naiara

; Justiniano, Wagner

Revista Ceres

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feb 2011, Volume 58 N. 1 Pages 35 - 42

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

O uso de misturas de glyphosate, em tanque, para manejo de espécies de plantas daninhas de difícil controle tem sido prática comum entre os agricultores brasileiros. Desta forma, este trabalho teve como objetivo avaliar a eficácia e seletividade de misturas, em tanque, de herbicidas com glyphosate para o controle de trapoeraba (Commelina benghalensis L.), erva-de-touro (Tridax procumbens L.) e capim-carrapicho (Cenchrus echinatus L.) na cultura da soja RR®. O experimento foi conduzido em Maracaí, São Paulo, no período de novembro de 2006 a março de 2007, utilizando-se o cultivar CD-214RR® e delineamento experimental de blocos ao acaso, com 21 tratamentos e quatro repetições. Os tratamentos foram constituídos da aplicação de: glyphosate (180; 360; 540 e 720 g ha-1); glyphosate em sequencial (180/360; 360/360 e 540/360 g ha-1); glyphosate + chlorimuron-ethyl 360+10; 540+10; 360+5/ 360+5 g ha-1); glyphosate + lactofen (360+120; 540+120; 360+60/ 360+60 g ha-1); glyphosate + cloransulam-methyl (360+30; 540+30; 360+16,9/ 360+12,9 g ha-1); glyphosate + carfentrazone (360+4 g ha-1); glyphosate + imazethapyr (360+50 g ha-1); glyphosate + imazethapyr (177,8+30 g ha-1) e testemunhas capinada e sem capina. Apesar da similaridade de produtividade de grãos entre os tratamentos com glyphosate isolado e sequencial, nas doses 540, 720 e 540/ 360 g ha-1, as misturas em tanque com chlorimuron-ethyl, cloransulam-methyl, lactofen e imazethapyr favoreceram o controle de espécies de plantas daninhas tolerantes ao glyphosate como C. benghalensis e T. procumbens.

Although the use of glyphosate tank mixtures for managing weed species of difficult control is prohibited by the Brazilian legislation, it has been a common practice among farmers. This work aimed to evaluate the efficiency and selectivity of herbicide tank mixtures using glyphosate to control Commelina benghalensis L., Tridax procumbens L. and Cenchrus echinatus L. in soybean crop RR®. A field experiment was conducted in the Municipality of Maracai, São Paulo State, between November, 2006 and March 2007, using the genotype CD-214RR® in a complete randomized block design with 21 treatments and four replications. The treatments consisted of the following applications: glyphosate (180; 360; 540 and 720 g ha-1); glyphosate in sequence (180/360; 360/360 and 540/360 g ha-1); glyphosate + chlorimuron-ethyl 360+10; 540+10 and 360+5/ 360+5 g ha-1); glyphosate + lactofen (360+120; 540+120 and 360+60/ 360+60 g ha-1); glyphosate + cloransulam-methyl (360+30; 540+30 and 360+16,9/ 360+12,9 g ha-1); glyphosate + carfentrazone (360+4 g ha-1); glyphosate + imazethapyr (360+50 g ha-1); glyphosate + imazethapyr (177.8+30 g ha-1) and controls with and without weeding. Despite the similarity in grain yield between treatments with glyphosate alone and sequentially at doses 540, 720 and 540 / 360 g ha-1, the tank mixtures with chlorimuron-ethyl, cloransulam-methyl, lactofen and imazethapyr favored control of weed species tolerant to glyphosate such as C. benghalensis and T. procumbens.

4750 downloads Cited 1 time in SciELO

14.

Nematóides resistentes a alguns anti-helmínticos em rebanhos caprinos no Cariri Paraibano
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Lima, Wagner C.

; Athayde, Ana C.R.

; Medeiros, Geovergue R.

; Lima, Dayanne A.S.D.

; Borburema, Jucileide B.

; Santos, Edson M.

; Vilela, Vinícius L.R.

; Azevedo, Sérgio S.

Pesquisa Veterinária Brasileira

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dec 2010, Volume 30 N. 12 Pages 1003 - 1009

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Objetivou-se avaliar a resistência de nematódeos a alguns anti-helmínticos em rebanhos caprinos no Cariri Paraibano. Foram utilizados 144 animais no período seco e 120 animais no período chuvoso, fêmeas com idade acima de oito meses de vida e lactantes, distribuídos em 4 grupos: Grupo I não tratado, Grupo II tratado com albendazole a 10%, Grupo III tratado com ivermectina a 1% e Grupo IV tratado com fosfato de levamisole a 18,8%, nas doses recomendadas pelos fabricantes. Para avaliar a resistência, aplicou-se o teste de redução na contagem de ovos por grama de fezes (RCOF) e o cultivo de larvas de helmintos. As amostras fecais foram coletadas no dia do tratamento (dia base) e 7, 14 e 21 dias após o tratamento. No grupo tratado com Albendazole, observaram-se eficácias de 61%, 11% e 24% no período seco e de 55%, 14% e 12% no período chuvoso, aos 7, 14, e 21 dias, respectivamente. No grupo tratado com Ivermectina, a eficácia foi de 14%, 70% e 66% para o período seco, e de 76%, 34% e 71% para o período chuvoso, aos 7, 14 e 21 dias, respectivamente. O grupo tratado com fosfato de Levamisole apresentou percentuais de eficácia de 89%, 79% e 73% no período seco e de 76%, 69% e 67% no período chuvoso, aos 7, 14 e 21 dias, respectivamente. Os resultados obtidos indicam que os nematódeos gastrintestinais de alguns rebanhos caprinos no Cariri Paraibano não são sensíveis aos princípios ativos Albendazole, Levamisole e Ivermectina. Durante o período de estudo foi identificada a presença de parasitas dos gêneros Haemonchus, Trichostrongylus, Oesophagostomum e Strongyloides.

The aim of this paper was to evaluate the resistance of nematodes to some anthelmintics in dairy goats in Cariri Paraibano, Brazil. A total of 144 female goats, over 8 months of age, were used in the dry season and 120 ones during the rainy season, divided into four groups: Group I untreated, Group II treated with albendazole 10%, Group III treated with ivermectin 1%, and Group IV treated with levamisole phosphate 18.8%, at doses recommended by the manufacturer. To evaluate the resistance, the reduction in egg count per gram of feces and the cultivation of larval helminths. The fecal samples were collected on the day of treatment and, after 7, 14 and 21 days of treatment. In the group treated with albendazole, 61%, 11% and 24% of efficiency was observed in the dry period, and 55%, 14% and 12% in the rainy season, at 7, 14, and 21 days respectively. In the group treated with ivermectin, efficacy was 14%, 70% and 66% for the dry period, and 76%, 34% and 71% for the rainy season, at 7, 14 and 21 days respectively. The group treated with phosphate Levamisole showed efficacy rates of 89%, 79% and 73% in the dry period, and 76%, 69% and 67% in the rainy season, at 7, 14 and 21 days. The results indicate that the gastrointestinal nematodes of some dairy goats in Cariri Paraibano are not sensitive to the active Albendazole, Levamisole and Ivermectin. During the study, presence of specimens of the genera Haemonchus, Trichostrongylus, Oesophagostomum and Strongyloides was identified.

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15.

Chemical constituents from the roots of Spathelia excelsa and their antiprotozoal activity
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Moreira, Wagner A. dos Santos

; Lima, Maria da Paz

; Ferreira, Antonio Gilberto

; Ferreira, Izabel C. Piloto

; Nakamura, Celso V.

Journal of the Brazilian Chemical Society

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2009, Volume 20 N. 6 Pages 1089 - 1094

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

A investigação fitoquímica das raízes de Spathelia excelsa levou ao isolamento das cromonas 10(2,3-epóxi-3-metilbutanil)spatheliacromeno e 10(2,3-diidroxi-3-metilbutanil) metoxispatheliacromeno (5-metoxispatheliabiscromeno); o limonóide desacetilspathelinae epímero C-21 do protolimonóide 3β-angeloiloxi-7α,24,25-triidroxi-21,23-óxido-14,18-cicloapotirucal-21-hemiacetal; os alcalóides 7,8-dimetoxiflindersina, casimiroinaeN-methyl-4,7,8-trimetoxiquinolino-2(1H)-ona, além da mistura de β-sitosterol e stigmasterol. Nos ensaios biológicos sobre a forma promastigosta de Leishmania braziliensis, desacetilspathelina apresentou atividade moderada e sobre a forma epimastigota de Trypanossoma cruzi, 10(2,3-epóxi-3-metilbutanil)spatheliacromeno apresentou atividade forte (IC50= 11 µg mL-1).

Phytochemical investigation from roots of Spathelia excelsa yielded the chromones 10(2,3-epoxy-3-methylbutanyl) spatheliachromen and 10(2,3-dihydroxy-3-methylbutanyl) methoxyspatheliacromen (5-methoxyspatheliabischromen); limonoid deacetylspathelin and protolimonoid C-21-epimers 3β-angeloyloxy-7α,24,25-trihydroxy-21,23-oxide-14,18-cycloapotirucall-21-hemiacetal; the alkaloids 7,8-dimethoxyflindersin, casimiroin and N-methyl-4,7,8-trimethoxyquinolin-2(1H)-one, besides a mixture of β-sitosterol and stigmasterol. Assays on promastigote forms of Leishmania braziliensis, deacetylspathelin showed moderate activity; and on epimastigote forms of Trypanossoma cruzi, 10(2,3-epoxy-3-methylbutanyl)spatheliachromen exhibited strong activity (IC50 = 11 µg mL-1).

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