Abstract Objective: To examine the mental health status and related factors in children and adolescents, and to assess age groups and sexes differences in factors influencing mental health. Methods: This cross-sectional study was performed on Chinese children aged 6-18 years from November 2021 to January 2022. Mental health difficulties were accessed by the Strengths and Difficulties Questionnaire. Multivariate logistic regression was used to analyze factors associated with mental health status. Multiple linear regression was used to evaluate factors associated with the scores of the Strengths and Difficulties Questionnaire. Results: The prevalence of mental health difficulties was 12.98% (n =1348). Age (OR, 0.909, [95%CI, 0.830-0.996]), sex (OR, 1.424, [95%CI, 1.033-1.963]) and screen time on weekdays (“≥2” h/d vs “< 1” h/d: OR, 2.001, [95%CI, 1.300-3.080]) were related factors for mental health difficulties. For children (year ≤ 12), the strongest related factor for mental health difficulties was screen time on weekdays (“≥ 2” h/d vs “< 1” h/d: OR, 1.821 [95%CI, 1.203-2.755]). The risk of mental health difficulties in females with ≥ 2 h/d screen time on weekends was 3.420 times higher than those with < 1 h/d (OR, 3.420, [95%CI, 1.923-6.081]). Conclusion: The prevalence of mental health difficulties among children and adolescents was relatively high. The lower age, female sex and excessive screen time were associated with a higher risk of mental health difficulties. The factors influencing mental health varied by different age groups and sexes. Thus, specific measures for different age groups and sexes should be adopted to mitigate the impact. Objective Methods crosssectional cross sectional 618 6 18 6-1 202 2022 Questionnaire Results 1298 12 98 12.98 n =1348. 1348 =1348 . =1348) OR (OR 0909 0 909 0.909 95%CI, 95CI CI 95 [95%CI 0.8300.996, 08300996 0.830 0.996 , 830 996 0.830-0.996]) 1424 424 1.424 1.0331.963 10331963 1.033 1.963 033 963 1.033-1.963] ≥2 (“≥2 hd h d “ 2001 001 2.001 1.3003.080 13003080 1.300 3.080 300 3 080 1.300-3.080] year 12, 12) (“ 1821 821 1.82 1.2032.755. 12032755 1.203 2.755 203 755 1.203-2.755]) 3420 420 3.42 1.9236.081. 19236081 1.923 6.081 923 081 1.923-6.081]) Conclusion high Thus impact 61 6- 20 129 9 12.9 134 =134 090 90 0.90 95%CI 8300 0.8300.996 0830099 0830 0.83 0996 0.99 83 99 0.830-0.996] 142 42 1.42 0331 1.0331.96 1033196 1033 1.03 1963 1.96 03 96 1.033-1.963 200 00 2.00 3003 1.3003.08 1300308 1300 1.30 3080 3.08 30 08 1.300-3.080 ( 182 82 1.8 2032 1.2032.755 1203275 1203 1.20 2755 2.75 75 1.203-2.755] 342 3.4 9236 1.9236.081 1923608 1923 1.92 6081 6.08 92 1.923-6.081] 12. 13 =13 09 0.9 0.8300.99 083009 083 0.8 099 8 0.830-0.996 14 4 1.4 1.0331.9 103319 103 1.0 196 1.9 1.033-1.96 2.0 1.3003.0 130030 130 1.3 308 3.0 1.300-3.08 1. 1.2032.75 120327 120 1.2 275 2.7 7 1.203-2.755 34 3. 1.9236.08 192360 192 608 6.0 1.923-6.081 =1 0. 0.8300.9 08300 0.830-0.99 1.0331. 10331 10 19 1.033-1.9 2. 1.3003. 13003 1.300-3.0 1.2032.7 12032 27 1.203-2.75 1.9236.0 19236 60 6. 1.923-6.08 = 0.8300. 0.830-0.9 1.0331 1.033-1. 1.3003 1.300-3. 1.2032. 1.203-2.7 1.9236. 1.923-6.0 0.8300 0.830-0. 1.033-1 1.300-3 1.2032 1.203-2. 1.9236 1.923-6. 0.830-0 1.033- 1.300- 1.203-2 1.923-6 0.830- 1.203- 1.923-

In this study, the typical azo dye methyl orange (MO) was degraded by pyrite (FeS2) activated by hydrogen peroxide (H2O2). When [MO] = 0.1 mM, [FeSJ = 2.0 g/L and [H2O2] = 22 mM, 96.4% MO was removed in 120 min and the TOC removal rate was higher than 50%. HO* was the primary radical responsible for MO degradation, in addition, the acid condition promoted the degradation of MO in the FeS2/H2O2 system. MO in tap water and river water was not effectively degraded, whereas acidification could weaken the inhibitory effect on the FeS2/ H2O2 system to enable the degradation of MO in tap and river water. The OD600 indicated that the solution was environmentally friendly after the reaction, and three degradation pathways of MO were discussed. In summary, Fe(ll) could be dissolved from FeS2, which activated H2O2 to generate Fe(lll) and HO*. FeS2 could reduce Fe(lll) into Fe(ll), thus realizing the Fe(lll)/(ll) cycle and efficiently activating H2O2 to degrade MO.

ABSTRACT Blue eggshells have become economically valuable due to their higher popularity among consumers when compared with eggs of other colors, particularly in East Asia. However, eggshell colors vary widely and gradually become lighter with the increase of age. Therefore, to determine the association between DNA methylation and gene expression on the molecular mechanism of eggshell color variation in Xuefeng Black-bone chickens, we collected the shell glands from chickens that produced dark blue eggshells and light blue eggshells, respectively, and performed whole genome bisulfite sequencing (WGBS) and RNA sequencing (RNA-Seq) on them. The results showed that in the context of CG, DNA methylation levels were the highest compared to CHG and CHH contexts, and were negatively correlated with gene expression levels in three different gene regions in all samples, while there was no significant correlation in the contexts of CHG and CHH. Furthermore, when disregarding the location of coding genes, a total of 55 genes will show differences not only in expression levels but also in DNA methylation levels, and among the majority of differential expression genes (n=50), the DNA methylation levels in the gene body region were the highest. GO analysis and KEGG pathway analysis were performed in these genes and the results showed that the genes NR1H4 and HEPHL1, ABCA4 and ABCA12, and the pathways, cell adhesion molecular, ABC transporters, ECM-receptor interaction, and neuroactive ligand-receptor interaction, may affect the color variation of the blue eggshell of Xuefeng Black-bone chickens. Asia However age Therefore Blackbone Black bone respectively WGBS (WGBS RNASeq Seq (RNA-Seq them CG samples Furthermore 5 n=50, n50 n n=50 , 50 (n=50) NRH NR H NR1H HEPHL1 HEPHL ABCA ABCA12 pathways transporters ECMreceptor ECM receptor interaction ligandreceptor ligand n5 n=5 (n=50 ABCA1 n= (n=5 (n= (n

This study aimed to analyze the safety and applicability of a 90-min duration of infusion (SDI) of obinutuzumab in patients with B-cell non-Hodgkin's lymphoma (NHL) in a tertiary hospital in China. This exploratory clinical trial was performed at Jiangsu Province Hospital. All patients were treated with the standard infusion regimen for the first infusion. If no grade ≥3 infusion-related reactions (IRRs) occurred, the subsequent infusions were given as SDI. The primary endpoint was the incidence of IRR during the standard infusion (3-4 h) and 90-min SDI regimens. This study enrolled 208 patients and all completed cycle 1. Forty-one patients (19.71%) had IRRs: five (2.40%) with grade 1, twenty-eight (13.46%) with grade 2, and eight (3.85%) with grade 3. The 41 patients had 71 IRRs, mainly fever (40.85%), chest pain/tightness (12.68%), and dyspnea (9.86%). The occurrence of IRRs in the first infusion was significantly lower in patients who received oral acetaminophen prophylaxis than those who did not (10.72% vs 30.21%, P<0.001). For the subsequent cycles with 90-min SDI, only two (0.25%) IRRs occurred among 814 infusions (one grade 1 hand numbness and one grade 2 chill/fever). The 90-min obinutuzumab SDI might be safe and feasible in patients with B-cell NHL in China. 90min min 90 (SDI Bcell B cell nonHodgkins non Hodgkin s (NHL China Hospital 3 ≥ infusionrelated related (IRRs 34 4 (3- h regimens 20 Fortyone Forty 19.71% 1971 19 (19.71% 2.40% 240 40 (2.40% twentyeight twenty 13.46% 1346 13 46 (13.46% 3.85% 385 85 (3.85% 7 40.85%, 4085 40.85% , (40.85%) paintightness pain tightness 12.68%, 1268 12.68% 12 68 (12.68%) 9.86%. 986 9.86% . 9 86 (9.86%) 10.72% 1072 10 72 (10.72 3021 30 21 30.21% P<0.001. P0001 P P<0.001 0 001 P<0.001) 0.25% 025 25 (0.25% 81 chill/fever. chillfever chill/fever chill chill/fever) (3 19.71 197 (19.71 2.40 24 (2.40 13.46 134 (13.46 3.85 38 8 (3.85 408 40.85 (40.85% 126 12.68 6 (12.68% 98 9.86 (9.86% 10.72 107 (10.7 302 30.21 P000 P<0.00 00 0.25 02 (0.25 ( 19.7 (19.7 2.4 (2.4 13.4 (13.4 3.8 (3.8 40.8 (40.85 12.6 (12.68 9.8 (9.86 10.7 (10. 30.2 P00 P<0.0 0.2 (0.2 19. (19. 2. (2. 13. (13. (3. 40. (40.8 12. (12.6 9. (9.8 10. (10 30. P0 P<0. 0. (0. (19 (2 (13 (40. (12. (9. (1 P<0 (0 (40 (12 (9 P< (4

The aim of this study was to evaluate the effects of seven different types of additives on the production performance and economic benefits of piglets at the nursery stage. A total of 2806 piglets, at ~21 days of age, were selected and divided into a high-bodyweight (H) group (> 6.5 kg) and a low-bodyweight (L) group (<6.0 kg) based on the weaned bodyweight (BW). Each group was further subdivided into seven additive groups and a control group. The control group was provided with basal diet, while the additive groups received the corresponding functional additives for either 15 or 30 d. Data collection continued until the end of the nursery stage (on the 45th day of the trial, at 70 d of age). Any single additive did not substantially improve the gained BW in nursery piglets. Overall, sex did not result in any BW differences at any time points. Supplementing feed with additives for 30 d led to better results in terms of production performance and economics for both H and L groups compared to 15 d, particularly with the fermented traditional Chinese medicine (additive VI). In comparison to the control group, the beneficial differences in the H group was more than double that observed in the L group. In summary, supplementing with functional additives for 30 d has potential to improve the production performance and economic benefits of nursery piglets, especially high-bodyweight piglets.

A series of 4H-pyran derivatives were synthesized through a three-component condensation reaction in a pot using aromatic aldehyde, cyclohexanedione, and malononitrile in the dodecyl benzenesulfonic acid/H2O microemulsion system. This protocol is attractive because dodecyl benzenesulfonic acid microemulsion is superior for organic reaction due to its dual catalysis. On the one hand, dodecyl benzenesulfonic acid acts as a Brønsted acid to catalyze the reaction; on the other hand, it can form a microemulsion as surfactant to enlarge the boundary area and entrap water produced during esterification reaction. In addition, the reaction process is simple, with high liaison formation effciency, good yields, and environmentally harmless reaction conditions. The dodecyl benzenesulfonic acid/H2O microemulsion system was equally effective when used in consecutive reactions. 4Hpyran Hpyran 4H pyran H threecomponent three component aldehyde cyclohexanedione acidH2O acidHO H2O O catalysis hand addition simple effciency yields conditions reactions acidH HO

A charge-assisted hydrogen-bonded organic framework (HOF) FJU-201 is constructed by N,N’-bis(5-isophthalic acid)naphthalimide divalent anion (H2L2-), p-xylene and dimethylammonium. FJU-201 can only crystallize in solutions containing p-xylene, and cannot crystallize in both o-xylene and m-xylene. FJU-201 can also crystallize in xylene mixtures with the content of p-xylene is not less than 50%, which can be used for separating and purifying p-xylene, and it has good recyclability. chargeassisted charge assisted hydrogenbonded hydrogen bonded HOF (HOF FJU201 FJU 201 FJU-20 N,Nbis5isophthalic NNbis5isophthalic NNbisisophthalic N,N bis 5 isophthalic N acidnaphthalimide acid naphthalimide H2L2, H2L2 HL , H L (H2L2-) pxylene p dimethylammonium pxylene, xylene, oxylene o mxylene. mxylene m xylene. m-xylene 50 50% recyclability FJU20 20 FJU-2 Nbis5isophthalic Nbis NNbis NN H2L (H2L2- FJU2 2 FJU- Nbisisophthalic (H2L2 (H2L

Abstract The osseous vascular endothelium encompasses a vast intricate framework that regulates bone remodeling. Osteoporosis, an age-associated systemic bone disease, is characterized by the degeneration of the vascular architecture. Nevertheless, the precise mechanisms underpinning the metamorphosis of endothelial cells (ECs) with advancing age remain predominantly enigmatic. In this study, we conducted a systematic analysis of differentially expressed genes (DEGs) and the associated pathways in juvenile and mature femoral ECs, utilizing data sourced from the Gene Expression Omnibus (GEO) repositories (GSE148804) and employing bioinformatics tools. Through this approach, we successfully discerned six pivotal genes, namely Adamts1, Adamts2, Adamts4, Adamts14, Col5a1, and Col5a2. Subsequently, we constructed a miRNA-mRNA network based on miRNAs displaying differential expression between CD31hiEMCNhi and CD31lowEMCNlow ECs, utilizing online repositories for prediction. The expression of miR-466i-3p and miR-466i-5p in bone marrow ECs exhibited an inverse correlation with age. Our in vivo experiments additionally unveiled miR-466i-5p as a pivotal regulator in osseous ECs and a promising therapeutic target for age-related osteoporosis. remodeling Osteoporosis ageassociated disease architecture Nevertheless (ECs enigmatic study DEGs (DEGs GEO (GEO GSE148804 GSE (GSE148804 tools approach Adamts1 Adamts Adamts2 Adamts4 Adamts14 Col5a1 Cola Col Col5a2 Subsequently miRNAmRNA miRNA mRNA CDhiEMCNhi CD hiEMCNhi CDlowEMCNlow lowEMCNlow prediction miR466i3p miRip miR 466i 3p i p miR466i5p 5p agerelated related osteoporosis GSE14880 (GSE14880 Col5a GSE1488 (GSE1488 GSE148 (GSE148 GSE14 (GSE14 GSE1 (GSE1 (GSE

Abstract Background: This randomized and controlled prospective study tested the hypothesis that closed-loop Target-Controlled Infusion (TCI) of propofol would be associated with better system performance when compared with open-loop controlled delivery of propofol. Methods: Patients scheduled for elective breast surgery were randomly assigned to two groups: a closed-loop group, in which propofol infusion was performed by a closed-loop TCI system that used the Bispectral Index (BIS) as a feedback parameter to titrate the rate of propofol infusion, and an open-loop group, in which propofol infusion was performed manually and guided by the bispectral index. Results: A total of 156 patients were recruited for this study (closed-loop group n = 79; open-loop group n = 77). The Global Score (GS) of the closed-loop group was lower than that of the open-loop group (34.3 and 42.2) (p = 0.044). The proportions of time with a BIS value between 40 and 60 were almost identical in the closed-loop group and the open-loop group (68.7 ± 10.6% and 66.7 ± 13.3%) (p = 0.318). The individuals in the closed-loop group consumed more propofol compared with those in the open-loop group (7.20 ± 1.65 mg.kg−1.h−1 vs. 6.03 ± 1.31 mg.kg−1.h−1, p < 0.001). No intraoperative recall, somatic events or adverse events occurred. No significant difference in heart rate was observed between the two groups (p = 0.169). Conclusion: The closed-loop protocol was associated with lower BIS variability and lower out-of-range BIS values, at the cost of a greater consumption of propofol when compared to the open loop group. Register number:ChiCTR-INR-17010399. Background closedloop closed TargetControlled Target Controlled (TCI openloop Methods (BIS index Results 15 79 77. 77 . 77) GS (GS 34.3 343 34 3 (34. 42.2 422 42 2 0.044. 0044 0.044 0 044 0.044) 4 6 68.7 687 68 7 (68. 106 10 10.6 667 66 66. 13.3% 133 13 0.318. 0318 0.318 318 0.318) 7.20 720 20 (7.2 165 1 65 1.6 mgkg1h1 mgkgh mg kg h mg.kg−1.h− vs 603 03 6.0 131 31 1.3 0.001. 0001 0.001 001 0.001) recall occurred 0.169. 0169 0.169 169 0.169) Conclusion outofrange out range values numberChiCTRINR17010399. numberChiCTRINR17010399 numberChiCTRINR number ChiCTR INR 17010399. 17010399 number:ChiCTR-INR-17010399 34. (34 42. 004 0.04 04 68. (68 10. 13.3 031 0.31 7.2 72 (7. 16 1. mgkg mgkg1h mg.kg−1.h 6. 000 0.00 00 016 0.16 numberChiCTRINR1701039 1701039 number:ChiCTR-INR-1701039 (3 0.0 (6 13. 0.3 7. (7 01 0.1 numberChiCTRINR170103 170103 number:ChiCTR-INR-170103 ( 0. numberChiCTRINR17010 17010 number:ChiCTR-INR-17010 numberChiCTRINR1701 1701 number:ChiCTR-INR-1701 numberChiCTRINR170 170 number:ChiCTR-INR-170 numberChiCTRINR17 17 number:ChiCTR-INR-17 numberChiCTRINR1 number:ChiCTR-INR-1 number:ChiCTR-INR- number:ChiCTR-INR

ABSTRACT Introduction: The aim of this study was to identify perioperative risk factors of laryngeal symptoms and to develop an implementable risk prediction model for Chinese hospitalized patients undergoing coronary artery bypass grafting (CABG). Methods: A total of 1476 Chinese CABG patients admitted to Wuhan Asian Heart Hospital from January 2020 to June 2022 were included and then divided into a modeling cohort and a verification cohort. Univariate analysis was used to identify laryngeal symptoms risk factors, and multivariate logistic regression was applied to construct a prediction model for laryngeal symptoms after CABG. Discrimination and calibration of this model were validated based on the area under the receiver operating characteristic (ROC) curve and the Hosmer-Lemeshow (H-L) test, respectively. Results: The incidence of laryngeal symptoms in patients who underwent CABG was 6.48%. Four independent risk factors were included in the model, and the established aryngeal complications risk calculation formula was Logit (P) = −4.525 + 0.824 × female + 2.09 × body mass index < 18.5 Kg/m2 + 0.793 × transesophageal echocardiogram + 1.218 × intensive care unit intubation time. For laryngeal symptoms, the area under the ROC curve was 0.769 in the derivation cohort (95% confidence interval [CI]: 0.698-0.840) and 0.811 in the validation cohort (95% CI: 0.742-0.879). According to the H-L test, the P-values in the modeling group and the verification group were 0.659 and 0.838, respectively. Conclusion: The prediction model developed in this study can be used to identify high-risk patients for laryngealsymptoms undergoing CABG, and help clinicians implement the follow-up treatment. Introduction . (CABG) Methods 147 202 (ROC HosmerLemeshow Hosmer Lemeshow HL H L (H-L test respectively Results 648 6 48 6.48% P (P 4525 4 525 −4.52 0824 0 824 0.82 209 2 09 2.0 185 18 5 18. Kgm2 Kgm Kg m2 m Kg/m 0793 793 0.79 1218 1 218 1.21 time 0769 769 0.76 95% 95 (95 CI [CI] 0.6980.840 06980840 0.698 0.840 698 840 0.698-0.840 0811 811 0.81 0.7420.879. 07420879 0.742 0.879 742 879 0.742-0.879) Pvalues values 0659 659 0.65 0838 838 0.838 Conclusion highrisk high followup follow up treatment (CABG 14 20 64 6.48 452 52 −4.5 082 82 0.8 2. 079 79 0.7 121 21 1.2 076 76 9 (9 [CI 6980 0.6980.84 0698084 0698 0.69 0840 0.84 69 84 0.698-0.84 081 81 7420 0.7420.879 0742087 0742 0.74 0879 0.87 74 87 0.742-0.879 065 65 0.6 083 83 0.83 6.4 45 −4. 08 8 0. 07 7 12 1. ( 0.6980.8 069808 069 084 0.698-0.8 0.7420.87 074208 074 087 0.742-0.87 06 6. −4 0.6980. 06980 0.698-0. 0.7420.8 07420 0.742-0.8 − 0.6980 0.698-0 0.7420. 0.742-0. 0.698- 0.7420 0.742-0 0.742-

Resumo: O atual ecossistema de mídia em rede é claramente caracterizado pela convergência. Recentemente, a convergência de mídia tem progredido rapidamente, tanto nos círculos teóricos quanto nas aplicações práticas, e o desenvolvimento da convergência tornou-se inevitavelmente uma tendência importante no desenvolvimento da mídia. A convergência de mídias transformou, de forma única, a ecologia das notícias, da comunicação e da opinião pública, na qual uma revisão da produção de notícias se destaca enquanto sua expressão principal. Como resultado da convergência da mídia, a produção de notícias mudou de um modo linear tradicional para um modo não linear. Devido ao uso de vídeo, áudio e formas H5 de notícias, na nova mídia, além da mídia tradicional, baseada principalmente em conteúdo de texto e imagem, as reportagens de notícias são agora mais diversificadas, visualmente atraentes e inovadoras. O processo de produção de notícias mudou como resultado da convergência da mídia, tornando-se mais eficaz, adaptável e diversificado. Ao estudar o impacto da integração da mídia sobre a produção de notícias, este artigo teve, por objetivo, entender como a mídia deve ser ajustada na era da integração, de que maneira a produção de notícias deve se desenvolver e se superar, e as leis gerais da produção de notícias no processo de desenvolvimento da integração. Ao mesmo tempo, nesse contexto, a pesquisa em questão investiga o impacto da convergência da mídia na produção de notícias, delineia a maneira como a mídia pode se adaptar a cenários dinâmicos, propõe novos métodos de produção de notícias para promover o progresso da era da convergência e visa estabelecer os princípios básicos da produção de notícias no desenvolvimento integrado. Sob a tendência de digitalização, rede e informatização, o modo de produção de notícias passou por mudanças. Com o surgimento da nova mídia e a transformação gradual da mídia tradicional, o ambiente e o modo de produção de notícias também passaram por mudanças profundas. Este artigo revelou os mecanismos subjacentes e as tendências futuras dessa mudança, que podem melhorar a eficiência e a qualidade da produção de notícias, e aumentar sua competitividade em um ambiente de mídia diversificado. Ele tem um valor de referência importante para organizações de notícias, profissionais de mídia e formuladores de políticas relevantes, podendo fornecer uma base científica para a tomada de decisões. Resumo Recentemente rapidamente práticas tornouse tornou transformou única pública principal vídeo H imagem diversificadas inovadoras tornandose tornando eficaz diversificado teve objetivo superar tempo contexto dinâmicos integrado digitalização informatização profundas mudança relevantes decisões

Abstract: The present-day networked media ecosystem is distinctly characterized by convergence. Recently, media convergence has experienced rapidly progress both in theoretical circles and in practical applications, and convergence development has inevitably become a leading trend in media development. Media convergence has uniquely transformed the ecology of news, communication and public opinion, in which an overhaul of news production stands out as its main expression. As a result of media convergence, news production has shifted from a traditional linear to a nonlinear mode. Due to the use of video, audio and H5 forms of news in new media, in addition to traditional media primarily based on text and image content, news reporting is now more diverse, visually appealing and innovative. The process of producing news has changed as a result of media convergence, becoming more effective, adaptable and diverse. By studying the impact of media integration on news production, this article aimed to understand how media should be adjusted in the era of integration, how news production should develop and break through, and the general laws of news production in the process of integration development. At the same time, in this context, the research under consideration investigates the impact of media convergence on news production, outlines the way that media can adapt to dynamic landscapes, proposes new methods of news production to promote the progress of the convergence era, and aims to establish the basic principles of news production in integrated development. Under the trend of digitization, networking and informatization, the mode of news production has undergone changes. With the rise of new media and the gradual transformation of traditional media, the news production environment and mode have also undergone profound changes. This article revealed the underlying mechanisms and future trends of this change, which can improve the efficiency and quality of news production and enhance its competitiveness in a diversified media environment. It has important reference value for news organizations, media practitioners and relevant policy makers, and can provide scientific basis for their decision-making. Abstract presentday present day Recently applications opinion expression video H content diverse innovative effective through time context landscapes digitization informatization changes change organizations makers decisionmaking. decisionmaking decision making. making decision-making

25-hydroxycholesterol (25-HC) plays a role in the regulation of cell survival and immunity. However, the effect of 25-HC on myocardial ischemia/reperfusion (MI/R) injury remains unknown. Our present study aimed to investigate whether 25-HC aggravated MI/R injury through NLRP3 inflammasome-mediated pyroptosis. The overlapping differentially expressed genes (DEGs) in MI/R were identified from the GSE775, GSE45818, GSE58486, and GSE46395 datasets in Gene Expression Omnibus (GEO) database. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses were conducted using the database of Annotation, Visualization and Integration Discovery (DAVID). The protein-protein interaction (PPI) network of the overlapping DEGs was established using the Search Tool for the Retrieval of Interacting Genes (STRING) database. These bioinformatics analyses indicated that cholesterol 25-hydroxylase (CH25H) was one of the crucial genes in MI/R injury. The oxygen-glucose deprivation/reoxygenation (OGD/R) cell model was established to simulate MI/R injury. Western blot and RT-qPCR analysis demonstrated that CH25H was significantly upregulated in OGD/R-stimulated H9C2 cardiomyocytes. Moreover, knockdown of CH25H inhibited the OGD/R-induced pyroptosis and nod-like receptor protein 3 (NLRP3) inflammasome activation, as demonstrated by cell counting kit-8 (CCK8), lactate dehydrogenase (LDH), RT-qPCR, and western blotting assays. Conversely, 25-HC, which is synthesized by CH25H, promoted activation of NLRP3 inflammasome in OGD/R-stimulated H9C2 cardiomyocytes. In addition, the NLRP3 inhibitor BAY11-7082 attenuated 25-HC-induced H9C2 cell injury and pyroptosis under OGD/R condition. In conclusion, 25-HC could aggravate OGD/R-induced pyroptosis through promoting activation of NLRP3 inflammasome in H9C2 cells. 25hydroxycholesterol hydroxycholesterol 25 25HC HC (25-HC immunity However ischemiareperfusion ischemia reperfusion MIR MI R (MI/R unknown NLRP inflammasomemediated mediated (DEGs GSE775 GSE GSE45818 GSE58486 GSE4639 GEO (GEO GO (GO KEGG (KEGG Annotation DAVID. DAVID . (DAVID) proteinprotein PPI (PPI STRING (STRING 25hydroxylase hydroxylase CHH CH H (CH25H oxygenglucose oxygen glucose deprivationreoxygenation deprivation reoxygenation OGDR OGD (OGD/R RTqPCR RT qPCR OGD/Rstimulated OGDRstimulated stimulated C H9C cardiomyocytes Moreover OGD/Rinduced OGDRinduced induced nodlike nod like (NLRP3 kit8 kit 8 kit- CCK8, CCK8 CCK , (CCK8) LDH, LDH (LDH) RTqPCR, qPCR, assays Conversely 25HC, HC, addition BAY117082 BAY BAY11 7082 BAY11-708 25HCinduced HCinduced condition conclusion cells 2 GSE77 GSE4581 GSE5848 GSE463 (DAVID Rstimulated Rinduced (NLRP (CCK8 (LDH BAY11708 BAY1 708 BAY11-70 GSE7 GSE458 GSE584 GSE46 (CCK BAY1170 70 BAY11-7 GSE45 GSE58 GSE4 BAY117 7 BAY11- GSE5

Tuberculosis (TB), caused by Mycobacterium tuberculosis, remains the leading cause of mortality by a single infectious agent in the world. M. tuberculosis infection could also result in clinical chronic infection, known as latent TB infection (LTBI). Compared to the current limited treatment, several subunit vaccines showed immunotherapeutic effects and were included in clinical trials. In this study, a subunit vaccine of Ag85B with a novel mucosal adjuvant c-di-AMP (Ag85B:c-di-AMP) was delivered intranasally to a persistent M. tuberculosis H37Ra infection mouse model, which also presented the asymptomatic characteristics of LTBI. Compared with Ag85B immunization, Ag85B:c-di-AMP vaccination induced stronger humoral immune responses, significantly higher CD4+ T cells recruitment, enhanced Th1/Th2/Th17 profile response in the lung, decreased pathological lesions of the lung, and reduced M. tuberculosis load in mice. Taken together, Ag85B:c-di-AMP mucosal route immunization provided an immunotherapeutic effect on persistent M. tuberculosis H37Ra infection, and c-di-AMP, as a promising potential mucosal adjuvant, could be further used in therapeutic or prophylactic vaccine strategies for persistent M. tuberculosis infection as well as LTBI. TB, , (TB) world M LTBI . (LTBI) treatment trials study AgB Ag B cdiAMP c di AMP Ag85BcdiAMP AgBcdiAMP (Ag85B:c-di-AMP HRa H Ra model responses CD4 CD recruitment Th1Th2Th17 ThThTh Th1 Th2 Th17 Th Th1/Th2/Th1 lung mice together cdiAMP, AMP, (TB (LTBI BcdiAMP Th1Th2Th1 Th1/Th2/Th Th1Th2Th

Abstract Background: NSCLC is one of the most common causes of death. The hypoxia microenvironment contributes to cancer progression. The purpose was to explore the effects and mechanism of melittin on NSCLC cells in the hypoxic microenvironment. Methods: NSCLC cell lines (A549 and H1299) were cultured in normoxia or hypoxia conditions with or without melittin treatment. The viability of the cells was detected via MTT assay and the proliferation ability was evaluated by EdU assay. QRT-PCR was performed to evaluate GLUT1, LDHA, HK2, VEGF and LATS2 mRNA levels. Glucose transport was assessed by the 2-NBDG uptake assay. The angiogenesis was determined by the tubule formation assay. The protein expressions of GLUT1, LDHA, HK2, VEGF, LATS2, YAP, p-YAP and HIF-1α were detected via western blotting assay. The tumor formation assay was conducted to examine the roles of melittin and LATS2 in vivo. Results: Melittin inhibited hypoxia-induced cell viability, proliferation, glycolysis and angiogenesis as well as suppressed YAP binding to HIF-1α in NSCLC. Melittin inactivated the YAP/HIF-1α pathway via up-regulation of LATS2, ultimately inhibiting cancer progression of NSCLC. Moreover, melittin suppressed tumor growth via up-regulation of LATS2 in vivo. Conclusion: Melittin inactivated the YAP/HIF-1α pathway via up-regulation of LATS2 to contribute to the development of NSCLC. Therefore, melittin is expected to become a potential prognostic drug for the therapy of NSCLC. Background death Methods A549 A (A54 H1299 H treatment QRTPCR QRT PCR GLUT1 GLUT LDHA HK2 HK LATS levels 2NBDG NBDG 2 pYAP p HIF1α HIFα HIF 1α α vivo Results hypoxiainduced induced YAP/HIF1α YAPHIF1α YAPHIFα YAP/HIF upregulation up regulation Moreover Conclusion Therefore A54 (A5 H129 YAPHIF A5 (A H12 H1

Abstract Objective: This study mainly explores (2R,6R; 2S,6S)-HNK and its compounds whether there are antidepressant effects. Methods: Four HNK compounds were obtained from 2-(Chlorophenyl) Cyclopentylmethanone. Forced swimming test, locomotor sensitization test, and conditioned location preference test were used to screen the antidepressant activity of the synthesized target compounds. Results: In the case of 10 mg HNK treatment, compared with saline, the immobile time of mice in the HNK group, I5 group and I6 group at 1 h and 7 days had statistical significance. In the case of 10 mg HNK treatment, compared with saline, the immobile time of compound C and D groups in the glass cylinder area was significantly different. In the locomotor sensitization test, the movement distance of compound C and D groups on day 15 and day 7 mice increased significantly compared with the first day. In the conditioned place preference experiment, compound C and compound D induced conditioned place preference in mice compared with the Veh group. Conclusion: The results of the forced swimming test, locomotor sensitization test, and conditioned location preference test showed that compounds C and D may have certain anti-depressant activity. However, HNK exerts a rapid and significant antidepressant effect within 1 week, but the duration is short. Objective 2R,6R 2R6R RR 2R 6R R (2R,6R 2S,6SHNK 2S6SHNK SSHNK 2S,6S 2S 6S S effects Methods 2Chlorophenyl Chlorophenyl 2 2-(Chlorophenyl Cyclopentylmethanone Results treatment saline I significance different experiment Conclusion anti depressant However week short 6SHNK SHNK 2S6S SS