ABSTRACT Tomato (Solanum lycopersicum L.), a globally significant species, faces substantial economic losses due to pest insects. However, exploiting dwarf germplasm for pest resistance, particularly the UFU MC TOM1 lineage, remains underexplored. The objectives of this study were to select backcross populations from dwarf tomato plants through direct and indirect selection and to elucidate whether there are consequences in resistance level as backcross generations progress aiming at a genetic background of interest. Backcross populations 1, 2, and 3 of dwarf tomato plants were obtained. In the conducted experiment, featuring 20 treatments and four repetitions in a randomized block design, we assessed foliar injuries and herbivory through image analysis. Additionally, we quantified the content of acylsugars, conducted chromatographic analysis, and analyzed the metabolomic profile in the leaflets. Promisingly, the UFU 13_1 dwarf plant population showed potential for enhancing genetic improvement programs targeting pest insect resistance. Moreover, the progression of backcross generations yielded varied resistance levels, likely influenced by inheritance patterns and acylsugar content. The UFU MC TOM1 line, distinguished by its high acylsugar content, exhibited robust resistance against both the tomato leafminer (Tuta absoluta Meyrick 1917) and the leafminer (Liriomyza huidobrensis Blanchard). Furthermore, our analysis revealed the expression of metabolites in the UFU MC TOM1 lineage, potentially linked to various biotic and abiotic stress responses. These findings offer promising prospects for leveraging the dwarf tomato variety, UFU MC TOM1, in genetic improvement programs aimed at combating pest insects in tomatoes. Solanum L., L L. , L.) species However TOM lineage underexplored interest 1 2 obtained experiment design Additionally acylsugars leaflets Promisingly 131 13 13_ Moreover levels line Tuta 1917 Liriomyza Blanchard. Blanchard . Blanchard) Furthermore responses variety tomatoes 191 19

RESUMO A obtenção de linhas de introgressão de tomateiro anão do tipo Saladete pode proporcionar vantagens em programas de melhoramento. Além do aumento de produtividade, a planta anã pode apresentar metabólitos para o estresse biótico. No entanto, não existe linhas de introgressão de tomateiro anão do tipo Saladete. O objetivo do trabalho foi avaliar o potencial agronômico, qualidade de fruto e metabólitos secundários para resistência a pragas visando a obtenção de linhas de introgressão de tomateiro anão do tipo Saladete. Foram avaliados 23 tratamentos, sendo o genitor doador UFU MC TOM 1, genitor recorrente UFU TOM 5, cultivar comercial Pizzadoro (Testemunha), cinco populações provenientes do primeiro e 15 populações do segundo retrocruzanento. Foram avaliadas características agronômicas e nutraceuticas dos frutos, e acilaçúcares nos folíolos. A dissimilaridade genética foi calculada por meio da distância generalizada de Mahalanobis (D2) e as estimativas de ganhos genéticos a partir dos índices de soma de ranks e da distância genótipo-ideótipo. Foi possível afirmar a importância de se obter o segundo retrocruzamento. As populações UFU_13_1, UFU_17_1, UFU_10_1, UFUi_11_3, UFUi_10_3 e UFU_11_2 possuem potencial para obtenção de linhas de introgressão por apresentarem boas características agronômicas, qualidade de frutos e teores de acilaçúcar similares a UFU MC TOM 1. O germoplasma de tomateiro anão possui variabilidade genética e background do tipo Saladete com potencial para explorar linhas de introgressão. A cultivar UFU MC TOM 1 é promissora e pode superar o acesso silvestre Solanum pennellii para programas de melhoramento visando resistência a pragas, produtividade e frutos biofortificados para carotenoides. biótico entanto agronômico 2 tratamentos 5 Testemunha, Testemunha , (Testemunha) retrocruzanento folíolos D2 D (D2 genótipoideótipo. genótipoideótipo genótipo ideótipo. ideótipo genótipo-ideótipo retrocruzamento UFU131 13 UFU_13_1 UFU171 17 UFU_17_1 UFU101 10 UFU_10_1 UFUi113 UFUi 11 3 UFUi_11_3 UFUi103 UFUi_10_ UFU112 UFU_11_ carotenoides (Testemunha (D UFU13 UFU_13_ UFU17 UFU_17_ UFU10 UFU_10_ UFUi11 UFUi_11_ UFUi10 UFUi_10 UFU11 UFU_11 UFU1 UFU_13 UFU_17 UFU_10 UFUi1 UFUi_11 UFUi_1 UFU_1 UFUi_ UFU_

ABSTRACT Obtaining introgression lines of saladete-type dwarf tomato plants can provide several advantages in breeding programs. In addition to increasing productivity, the dwarf plant can produce metabolites that are important to resistance to biotic stress. However, there are no saladette-type dwarf tomato introgression lines. The objective of this study was to evaluate the agronomic potential, fruit quality, and secondary metabolites associated with pest resistance for the development saladette-type dwarf tomato introgression lines. The experiment was conducted with 23 treatments, including the UFU MC TOM 1 donor parent, the UFU TOM 5 recurrent parent, the Pizzadoro commercial cultivar (control), 5 populations from the first backcross, and 15 populations from the second backcross. Agronomic and nutraceutical characteristics of fruits and the acylsugar content in the leaflets were evaluated. The genetic dissimilarity was calculated using the generalized Mahalanobis distance (D2). Genetic gain through selection was estimated using the rank sum index and the genotype-ideotype distance index. The selection indices showed the importance of obtaining the second backcross. The populations UFU_13_1, UFU_17_1, UFU_10_1, UFUi_11_3, UFUi_10_3, and UFU_11_2 have the potential to obtain introgression lines as they present good agronomic and fruit quality characteristics and acylsugar content similar to UFU MC TOM 1. The dwarf tomato germplasm obtained has significant genetic variability and a saladette-type genetic background with the potential to develop introgression lines. The cultivar UFU MC TOM 1 is promising and can overcome the wild access Solanum pennellii for breeding programs aimed at pest resistance, increasing productivity, and biofortification of fruits to enhance carotenoids. saladetetype saladete type productivity stress However saladettetype saladette 2 treatments parent control, control , (control) backcross evaluated D2. D2 D . (D2) genotypeideotype genotype ideotype UFU131 13 UFU_13_1 UFU171 17 UFU_17_1 UFU101 10 UFU_10_1 UFUi113 UFUi 11 3 UFUi_11_3 UFUi103 UFUi_10_3 UFU112 UFU_11_ carotenoids (control (D2 UFU13 UFU_13_ UFU17 UFU_17_ UFU10 UFU_10_ UFUi11 UFUi_11_ UFUi10 UFUi_10_ UFU11 UFU_11 (D UFU1 UFU_13 UFU_17 UFU_10 UFUi1 UFUi_11 UFUi_10 UFU_1 UFUi_1 UFU_ UFUi_