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Resumen Introducción: El trasplante renal es el tratamiento de elección de la enfermedad renal en etapa terminal (ERT). Desde marzo de 2020, la actividad de trasplantes en México se ha visto afectada debido a la pandemia de COVID-19. Objetivo: Determinar el impacto en la mortalidad de pacientes en lista de espera (LE) para trasplante renal de donante cadavérico en un hospital de referencia en Yucatán, por suspensión de actividades debido a la pandemia. Material y métodos: Pacientes > 18 años en LE para trasplante renal en este hospital. En caso de muerte de un paciente, se investigó la causa, especialmente si estaba asociada a COVID-19. Un valor de p de dos colas ≤ 0.05 se consideró significativo en todos los análisis. Resultados: La razón de probabilidad de muerte por COVID-19 en un paciente con ERT en la LE en 2020 fue OR = 5.04 (IC 95%: 1.65-7.14, p = 0.023). La razón de probabilidad de morir con ERT en la LE con retraso en las consultas de seguimiento fue de OR = 6.59 (IC 95%: 2.7-16.28, p = 0.008). Conclusión: La probabilidad de muerte de un paciente con ERT en la LE con retraso en las consultas de seguimiento y retención del trasplante es estadísticamente más alta que la probabilidad de muerte por COVID-19.

Abstract Introduction: Kidney transplantation is the treatment of choice for end-stage renal disease (ESRD). Since March 2020, transplant activity in Mexico has been affected due to the COVID-19 pandemic. Objective: The aim of the study was to determine the impact on mortality of patients on the waiting list (WL) for cadaveric donor kidney transplantation in a referral hospital in Yucatán, due to suspension of activities due to the pandemic. Material and methods: Patients over 18 years of age on the waiting list for kidney transplantation at this hospital. In the event of a patient's death, the cause was investigated, especially if it was associated with COVID-19. A two-tailed p ≤ 0.05 was considered significant in all analyzes. Results: The odds ratio (OR) of death from COVID-19 in a patient with ESRD in the WL in 2020 was OR = 5.04 (95% CI: 1.65-7.14, p = 0.023). The OR of dying with ESRD in the WL with a delay in the follow-up visits was OR = 6.59 (95% CI: 2.7-16.28, p = 0.008). Conclusion: The probability of death of a patient with ESRD with delayed follow-up visits and transplant retention is statistically higher than the probability of death from COVID-19.

https://doi.org/10.24875/ciru.21000718

Agreement between measured weight, height and BMI and Web-based self-reported data in participants of the 1993 Pelotas Birth Cohort, Brazil: a cross-sectional validation study weight Webbased Web based selfreported self reported 199 Cohort Brazil crosssectional cross sectional 19 1
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Botelho, Vivian Hernandez

; Barros, Aluísio J. D.

; Oliveira, Renata Gonçalves de

; Martins, Rafaela Costa

; Gonçalves, Helen

; Menezes, Ana M. B.

; Blumenberg, Cauane

Epidemiologia e Serviços de Saúde

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2023, Volume 32 N. 2 elocation e2022590

Abstract: Es En Pt | Text: Es En Pt | PDF: Es | PDF: En | PDF: Pt

RESUMEN Objetivo: evaluar la concordancia entre la altura, el peso y el índice de masa corporal (IMC) medidos durante el acompañamiento de 22 años de la cohorte de nacimientos de Pelotas de 1993 y autoinformado durante el seguimiento en línea de la coortesnaweb. Métodos: estudio metodológico de validación. Se utilizó el coeficiente de correlación de Lin para medidas continuas, y de Kappa ponderado para el estado nutricional y la correlación de Spearman para la correlación entre medidas. Resultados: se incluyeron 783 participantes, con alta correlación y concordancia entre las medidas de talla (r = 0,966; ρ = 0,966), peso (r = 0,934; ρ = 0,928) e IMC (r = 0,903; ρ = 0,910) medidos y autoinformados vía web. No hubo correlación entre las diferencias de medidas y el intervalo de tiempo entre las mediciones. Conclusión: el uso de internet para recopilar variables antropométricas autoinformadas es válido en comparación con el método tradicional. Objetivo altura (IMC 2 199 coortesnaweb Métodos validación continuas Resultados 78 participantes r 0,966 0966 0 966 0,966, , 0,966) 0,934 0934 934 0,928 0928 928 0,903 0903 903 0,910 0910 910 web mediciones Conclusión tradicional 19 7 0,96 096 96 0,93 093 93 0,92 092 92 0,90 090 90 0,91 091 91 1 0,9 09 9 0,

ABSTRACT Objective: to evaluate the agreement between measured height, weight, and body mass index (BMI) during the 22-year follow-up of the 1993 Pelotas Birth Cohort, state of Rio Grande do Sul, Brazil, and self-reported data during the online follow-up of the coortesnaweb. Methods: this was a cross-sectional validation study; agreement was assessed by means of Lin’s concordance correlation coefficient for continuous measures and weighted Kappa for nutritional status; Spearman’s rank correlation coefficient was used to estimate the correlation between measurements. Results: a total of 783 participants were included; it could be seen high correlation and high agreement between the measured height (r = 0.966; ρ = 0.966), weight (r = 0.934; ρ = 0.928), and BMI (r = 0.903; ρ = 0.910) and Web-based self-reported data; there was no correlation between mean difference and the time interval between measurements. Conclusion: using the Internet to collect self-reported anthropometric measurements is as valid as the traditional method. Objective (BMI 22year year 22 followup follow up 199 Cohort Sul Brazil selfreported self reported coortesnaweb Methods crosssectional cross sectional study Lins Lin s status Spearmans Spearman Results 78 included r 0.966 0966 0 966 0.966, , 0.966) 0.934 0934 934 0.928, 0928 0.928 928 0.928) 0.903 0903 903 0.910 0910 910 Webbased Web based Conclusion method 2 19 7 0.96 096 96 0.93 093 93 092 0.92 92 0.90 090 90 0.91 091 91 1 0.9 09 9 0.

Resumo Objetivo: avaliar a concordância entre altura, peso e índice de massa corporal (IMC) aferidos durante o acompanhamento dos 22 anos da coorte de nascimentos de Pelotas, Rio Grande do Sul, Brasil, de 1993, e dados autorrelatados durante o acompanhamento online da coortesnaweb. Métodos: estudo transversal de validação; a concordância foi avaliada pelos coeficientes de correlação de Lin para medidas contínuas e de Kappa ponderado para o estado nutricional; utilizou-se a correlação de Spearman para estimar a correlação entre as medidas. Resultados: 783 participantes foram incluídos; observou-se alta correlação e alta concordância entre as medidas de altura (r = 0,966; ρ = 0,966), peso (r = 0,934; ρ = 0,928) e IMC (r = 0,903; ρ = 0,910) aferidas e as autorrelatadas via internet; não houve correlação entre as diferenças médias e o intervalo de tempo entre as medidas. Conclusão: utilizar a internet para coletar medidas antropométricas autorrelatadas é um método válido, comparado ao método tradicional. Objetivo (IMC 2 Pelotas Sul Brasil 1993 coortesnaweb Métodos validação nutricional utilizouse utilizou se Resultados 78 incluídos observouse observou r 0,966 0966 0 966 0,966, , 0,966) 0,934 0934 934 0,928 0928 928 0,903 0903 903 0,910 0910 910 Conclusão válido tradicional 199 7 0,96 096 96 0,93 093 93 0,92 092 92 0,90 090 90 0,91 091 91 19 0,9 09 9 1 0,

https://doi.org/10.1590/s2237-96222023000200013

Agreement between measured weight, height and BMI and Web-based self-reported data in participants of the 1993 Pelotas Birth Cohort, Brazil: a cross-sectional validation study
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Botelho, Vivian Hernandez

; Barros, Aluísio J. D.

; Oliveira, Renata Gonçalves de

; Martins, Rafaela Costa

; Gonçalves, Helen

; Menezes, Ana M. B.

; Blumenberg, Cauane

Epidemiologia e Serviços de Saúde

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2023, Volume 32 N. 2 elocation e2022590

Abstract: Pt Es En | Text: Pt Es En | PDF: Pt | PDF: Es | PDF: En

https://doi.org/10.1590/s2237-96222023000200013

Evaluation of MALDI-TOF MS System for the Identification and Differentiation of Burkholderia cepacia Complex Species
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Volpato, Fabiana Caroline Zempulski

; Hernandez, Mayana Kieling

; Lima-Morales, Daiana de

; Wink, Priscila Lamb

; Martins, Daniela de Souza

; Oliveira, Katia Ruschel Pilger de

; Barth, Afonso Luís

Brazilian Archives of Biology and Technology

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2022, Volume 65 elocation e22210572

Abstract: En | Text: En | PDF: En

Abstract To evaluate the performance of MALDI-TOF MS system as a tool for identification and differentiation of Burkholderia cenocepacia from other species of Burkholderia cepacia complex (Bcc). Fifty-three suggestive colonies were submitted to the MALDI-TOF Microflex LT 4.0® (Bruker Daltonik, Bremen, Germany) system for identification. We compared two protocols of protein extraction: (A) Direct Method and (B) Tube Extraction. In parallel, all isolates were subjected to molecular diagnosis (primers for recA gene) to identify species belonging to Bcc and to differentiate B. cenocepacia in genomovar IIIA or IIIB. MALDI-TOF was able to identify all isolates to the genus level and 94.3% (50/53) to species using both methods. The MALDI-TOF system was able to identify 38 out of the 40 isolates identified as B. cenocepacia by molecular techniques. In conclusion, MALDI-TOF Microflex LT 4.0® is a feasible technique and presents a low cost of reagents; it can be used for the reliable differentiation of B. cenocepacia from other species of Bcc.

https://doi.org/10.1590/1678-4324-2022210572

Efficacy of Virtual Reality in Neurorehabilitation of Spinal Cord Injury Patients: A Systematic Review
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Orsatti-Sánchez, B. A.

; Diaz-Hernandez, O.

Revista mexicana de ingeniería biomédica

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aug 2021, Volume 42 N. 2 elocation 1141

Abstract: En Es | Text: En Es | PDF: En | PDF: Es

ABSTRACT This systematic review (SR) analyzed the effectiveness of interventions using virtual reality (VR) technology as a neurorehabilitation therapy in people with spinal cord injury (SCI). The SR was developed under the guidelines of the PRISMA statement and the recommendations of the Cochrane Collaboration, along with the PEDro and National Institute of Health scales to assess the risk of bias and methodological quality. The Cochrane, IEEE, BVS/LILACS, MEDLINE/PubMed, and Web of Science databases were browsed to identify studies that, between 2010 and 2020, evaluated the efficacy of these therapies. Out of 353 retrieved studies, 11 were finally selected after the application of the defined inclusion and exclusion criteria. These articles presented good methodological quality as they were mostly controlled clinical trials that analyzed mixed therapies with conventional therapies. Interventions based on non-immersive or immersive VR technology that achieved functional motor, balance, and psycho-emotional health improvement with positive effects on motivation, self-confidence, commitment, and active participation were identified in a total sample of 155 SCI patients. It was concluded that such VR technology is an effective tool of neurorehabilitation complementary to conventional therapies, which promotes functional improvement in SCI patients both in the clinic and at home.

RESUMEN Esta revisión sistemática (RS) analizó la eficacia de las intervenciones que utilizan la tecnología de realidad virtual (RV) como terapia de neurorrehabilitación en personas con lesión de médula espinal (LME). La RS fue desarrollada bajo los lineamientos de la declaración PRISMA y las recomendaciones de la Colaboración Cochrane, junto con las escalas de PEDro y del National Institute of Health para evaluar el riego de sesgo y la calidad metodológica. Se revisaron las bases de Cochrane, IEEE, BVS/LILACS, MEDLINE/PubMed y Web of Science para identificar estudios que, entre 2010 y 2020, evaluaron la eficacia de dichas terapias. De 353 estudios recuperados, 11 fueron finalmente seleccionados tras la aplicación de los criterios de inclusión y exclusión definidos. Dichos artículos presentaron una buena calidad metodológica, al ser mayormente ensayos clínicos controlados que analizaron terapias mixtas con terapias convencionales. Se identificaron intervenciones basadas en tecnología de RV no inmersiva o inmersiva que lograron una mejora funcional motora, de equilibrio y de salud psico-emocional con efectos positivos de motivación, seguridad, compromiso y activa participación en una muestra total de 155 pacientes con LME. Se concluyó que dicha tecnología de RV es una herramienta eficaz de neurorrehabilitación complementaria a las terapias convencionales, al promover una mejora funcional en pacientes con LME tanto en la clínica como en casa.

https://doi.org/10.17488/rmib.42.2.8 218 downloads

EFFECT OF THE POSTHARVEST PROCESSING METHOD ON THE BIOCHEMICAL COMPOSITION AND SENSORY ANALYSIS OF ARABICA COFFEE
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Rodriguez, Yeison F. B.

; Guzman, Nelson G.

; Hernandez, Joel G.

Engenharia Agrícola

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apr 2020, Volume 40 N. 2 Pages 177 - 183

Abstract: En | Text: En | PDF: En

ABSTRACT The objective of this work was to study the influence of two postharvest processing methods on the biochemical composition and sensory analysis of a Colombian specialty coffee. For this purpose, coffee beans (Coffea arabica) of the Castillo variety were collected and processed using the wet and semi-dry methods, and the drying process was conducted in a sun drier prototype with a plastic parabolic cover, with a maximum drying air temperature of 50 °C; green coffee beans were managed according to the official Specialty Coffee Association cupping protocol and roasted to a medium and dark degree. Physicochemical determinations (pH, color, titratable acidity, caffeine content, and chlorogenic acid quantification) in green coffee and roasted coffee beans were performed; the sensory analysis was performed by a panel of trained tasters. The wet and semi-dry processing methods resulted in differences in color parameters, pH values, and caffeine content. However, the titratable acidity, chlorogenic acid, and sensory evaluation did not have any difference; therefore, the type of coffee processing method used is not relevant to the final chemical properties of the roasted coffee (i.e., the Castillo variety) evaluated in this study; the coffee cup score is also not affected, and the post-fermentation washing process can be dispensed with.

https://doi.org/10.1590/1809-4430-eng.agric.v40n2p177-183/2020 1886 downloads

SDSS IV - Manga properties of AGN Host Galaxies
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Sánchez, S. F.

; Avila-Reese, V.

; Hernandez-Toledo, H.

; Cortes-Suárez, E.

; Rodríguez-Puebla, A.

; Ibarra-Medel, H.

; Cano-Díaz, M.

; Barrera-Ballesteros, J. K.

; Negrete, C. A.

; Calette, A. R.

; Lorenzo-Cáceres, A. de

; Ortega-Minakata, R. A.

; Aquino, E.

; Valenzuela, O.

; Clemente, J. C.

; Storchi-Bergmann, T.

; Riffel, R.

; Schimoia, J.

; Riffel, R. A.

; Rembold, S. B.

; Brownstein, J. R.

; Pan, K.

; Yates, R.

; Mallmann, N.

; Bitsakis, T.

Revista mexicana de astronomía y astrofísica

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2018, Volume 54 N. 1

Abstract: En Es | Text: En Es | PDF: En | PDF: Es

Abstract We present the characterization of the main properties of a sample of 98 AGN host galaxies, both type-II and type-I, in comparison with those of ≈2700 non-active galaxies observed by the MaNGA survey. We found that AGN hosts are morphologically early-type or early-spirals. AGN hosts are, on average, more massive, more compact, more centrally peaked and more pressure-supported systems. They are located in the intermediate/transition region between star-forming and non-starforming galaxies (i.e., the so-called green valley). We consider that they are in the process of halting/quenching the star formation. The analysis of the radial distributions of different properties shows that the quenching happens from inside-out involving both a decrease of the efficiency of the star formation and a deficit of molecular gas. The data-products of the current analysis are distributed as a Value Added Catalog within the SDSS-DR1411.

Resumen Presentamos la caracterización de las propiedades de 98 galaxias que albergan AGN, tanto de Tipo-I como de Tipo-II, en comparación con ≈2700 galaxias no activas extraídas del mapeado MaNGA. Las galaxias con AGN son de tipo temprano y espirales tempranas. Las galaxias con AGN son, en promedio, más masivas, compactas y concentradas en las partes centrales que las no activas, además de estar más soportadas por presión. Las galaxias con AGN se encuentran en la zona de transición entra las galaxias con formación estelar y las que no forman estrellas (valle verde), estando en el proceso de inhibir/detener su formación estelar. Las distribuciones radiales de diferentes propiedades muestran que esta inhibición ocurre desde dentro hacia fuera, debido a un decremento tanto de la eficiencia de la formación estelar como del gas molecular. Los productos de este análisis están disponibles como un Catálogo de Valor Añadido incluido en el SDSS-DR1411.

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Producción de ácido jasmónico por fermentación líquida con cepas de Botryodiplodia theobromae nativas del sureste mexicano
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Laredo-Alcalá, Elan I.

; Martínez-Hernandez, José L.

; Guillen-Cisneros, Lourdes

; Hernández-Castillo, Francisco D.

Agrociencia

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dec 2017, Volume 51 N. 8 Pages 885 - 893

Abstract: En Es | Text: En Es | PDF: En | PDF: Es

Abstract Jasmonic acid (JA) is an endogenous plant growth-regulating hormone found in plant species. It is involved in functions such as senescence and resistance. Plants produce these after damage due to pathogenic microorganisms or insects. An alternative for its production is to use microorganisms, the most used is Botryodiplodia theobromae. The objective of our study was to evaluate the JA production via liquid fermentation with B. theobromae strains, isolated from tropical areas of southeastern Mexico. The experimental design was completely random; the evaluated variable was the JA production capacity for each strain. The data were analyzed with the ANDEVA, and the means compared by the Tukey test (p≤0.05), for which we used the SAS statistical software. Likewise, we developed a kinetic production study with the most JA producing strain through pH, substrate consumption, biomass, and JA production. Twenty B. theobromae strains were isolated from cocoa (Theobroma cacao L.), passion fruit (Passiflora edulis L.), mango (Mangifera indica L.), coconut (Cocos nucifera L.) and papaya (Carica papaya L.) in experimental fields at Veracruz and Tabasco, Mexico. Five of these strains produced JA. Metabolites started to be produced from day ten of the bioreaction. Thus, we show that the phytopathogenic fungus of tropic B. theobromae can produce JA in a liquid fermentation system.

Resumen El ácido jasmónico (AJ) es una hormona endógena reguladora del crecimiento de plantas en las especies vegetales. Interviene en senescencia y resistencia y lo produce la planta después del daño ocasionado por microorganismos o insectos patógenos. Una alternativa para su producción es usar microorganismos y el más usado es Botryodiplodia theobromae. El objetivo de este estudio fue evaluar la producción de AJ mediante fermentación líquida con cepas de B. theobromae, aisladas de zonas tropicales del sureste de México. El diseño experimental fue completamente al azar y evaluamos la capacidad de producción de AJ de cada cepa.Los datos se analizaron con ANDEVA y las medias se compararon con la prueba de Tukey (p≤0.05) para lo cual se usó el programa SAS. Un estudio cinético de producción se desarrolló con la cepa más productora de AJ mediante evaluación de pH, consumo de sustrato, biomasa y producción de AJ. Veinte cepas de B. theobromae se aislaron desde cacao, maracuyá, mango, coco y papaya en campos experimentales de los estados de Veracruz y Tabasco, México. Cinco de esas cepas produjeron AJ y el metabolito inicio su producción desde el día 10 de bioreacción. Así, se mostró que el hongo fitopatógeno de zonas tropicales B. theobromae puede producir AJ en un sistema de fermentación líquida.

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Water productivity using SAFER - Simple Algorithm for Evapotranspiration Retrieving in watershed
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Coaguila, Daniel N.

; Hernandez, Fernando B. T.

; Teixeira, Antônio H. de C.

; Franco, Renato A. M.

; Leivas, Janice F.

Revista Brasileira de Engenharia Agrícola e Ambiental

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aug 2017, Volume 21 N. 8 Pages 524 - 529

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO A Bacia Hidrográfica do Córrego Cabeceira Comprida localizada no município de Santa Fé do Sul-SP, tem grande importância ambiental, sendo imprescindível para o abastecimento de água à população e para gerar excedentes para a diluição do esgoto gerado. Assim, este trabalho avaliou o comportamento anual dos componentes da produtividade da água a partir de imagens do Landsat-8 do ano 2015, utilizando-se o ‘Simple Algorithm for Evapotranspiration Retrieving’ (SAFER), sendo calculadas a evapotranspiração atual (ETa), a biomassa (BIO) e a produtividade da água (PA). As médias anuais da ETa, BIO e PA foram 1,03 mm dia-1, 36,04 kg ha-1 dia-1 e 3,19 kg m-3, respectivamente. As ETa médias anuais por uso e ocupação do solo foram 1,40, 1,23, 1,05, 0,97 e 1,08 mm dia-1 para mata remanescente (MR), espécies invasoras (EI), pastagem (Pa), cultura anual (CA) e cultura perene (CP), respectivamente, enquanto que as BIO apresentaram 57,64, 46,10, 36,78, 32,69, 40,03 kg ha-1 dia-1 para MR, EI, Pa, CA e CP, respectivamente, resultando em uma PA de 3,94, 3,59, 3,25, 3,09, 3,35 kg m-3 para MR, EI, Pa, CA e CP, respectivamente. As ETa, BIO e PA se ajustam à sazonalidade climática da região destacando-se a MR e as EI com os maiores valores.

ABSTRACT The Cabeceira Comprida stream’s watershed, located in Santa Fé do Sul, São Paulo state, has great environmental importance. It is essential for supplying water to the population and generating surpluses for sewage dilution. This study aimed to evaluate the annual performance of the components of water productivity from Landsat-8 images of 2015, using the Simple Algorithm for Evapotranspiration Retrieving (SAFER), calculating the actual evapotranspiration (ETa), biomass (BIO) and water productivity (WP). The annual averages of ETa, BIO and WP were 1.03 mm day-1, 36.04 kg ha-1 day-1 and 3.19 kg m-3, respectively. The average annual values of ETa for land use and occupation were 1.40, 1.23, 1.05, 0.97 and 1.08 mm day-1 for the remaining forest (RF), invasive species (IS), pasture (Pa), annual crop (AC) and perennial crop (PC), respectively, with BIO of 57.64, 46.10, 36.78, 32.69, 40.03 kg ha-1 day-1 for RF, IS, Pa, AC and PC, respectively, resulting in WP of 3.94, 3.59, 3.25, 3.09, 3.35 kg m-3 for RF, IS, Pa, AC and PC, respectively. The ETa, BIO and WP adjust to the seasonality of the region, and RF and IS stood out with the highest values.

https://doi.org/10.1590/1807-1929/agriambi.v21n8p524-529 2055 downloads

10.

A STUDY OF THE IMPACT OF LAND USE AND OCCUPATION ON BASIN WATER QUALITY THROUGH MULTIVARIATE STATISTICS
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Santos, Gilmar O.

; Hernandez, Fernando B. T.

; Ferraudo, Antonio S.

; Vanzela, Luiz S.

; Santos, Duílio J. O.

Engenharia Agrícola

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jun 2017, Volume 37 N. 3 Pages 453 - 462

Abstract: En | Text: En | PDF: En

ABSTRACT Without effective actions to improve environmental management, water resources tend to become scarce. Thus, this study aimed to understand the processes influencing the physical, chemical, and biological properties of the water due to land uses and occupation in the Ipê stream basin, in Ilha Solteira - SP (Brazil), regarding the dependence of the analyzed variables. Monitoring of chemical, physical, and biological water quality was performed from 2006 to 2011, and the records of land use and occupation in 2011. A factorial multivariate analysis allowed us to understand that the processes in densely populated areas increased degradation of water by the input of organic loads, yet those of farming areas enhanced the degradation of the chemical and physical quality of spring waters. Therefore, installing irrigation systems near populated areas, for crops consumed in natura, may compromise this agricultural activity, as well as the absence of water filtration systems downstream agricultural areas. Furthermore, we concluded that multivariate statistics is a powerful tool to detect these influencing processes in water quality. In this study, this type of analysis played an important role isolating the processes acting on water quality.

https://doi.org/10.1590/1809-4430-eng.agric.v37n3p453-462/2017 1127 downloads

11.

Cutaneous lymphoma of the tarsus in a cat - case study
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Sierra M, Oscar

; Viéra, Rafaela

; Ferreira, Marília

; Pascoli, Ana

; Reis, Nazilton

; Jark, Paulo

; Vargas-Hernandez, Giovanni

; Tinucci-Costa, Mirela

; De Nardi, Andrigo

; Calazans, Sabryna

Revista MVZ Córdoba

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apr 2017, Volume 22 N. 1 Pages 5747 - 5754

Abstract: Es En | Text: Es En | PDF: Es | PDF: En

RESUMEN El linfoma representa la enfermedad linfoproliferativa más frecuente en gatos. Sin embargo, el linfoma cutáneo es una rara forma de linfoma extranodal. Recientemente, el linfoma cutáneo se reportó localizado en el tarso. Tanto clínica como histopatológicamente, esta forma difiere de la forma cutánea típica, y se denominó linfoma tarsal felino. Este estudio describe el caso de un gato, doméstico de pelo largo, 13 años de edad con una masa subcutánea, de 30 días de evolución en la región tarsal del miembro pélvico derecho. La histopatologia reveló neoplasia de células redondas, sugestiva de linfoma de células grandes. La inmunohistoquímica confirmó el diagnóstico de linfoma imunoblástico de células B. El tratamiento realizado fue lomustina y prednisolona con tiempo de sobrevida de 2,1 meses. La agresividad de este linfoma, refuerza la necesidad de estudios para entender su curso y mejorar protocolos terapéuticos que incrementen tanto la sobrevida como la calidad de vida para estos pacientes.

ABSTRACT Lymphoma is the most common lymphoproliferative disorder in cats. Cutaneous lymphoma, however, is a rare form of extranodal lymphoma. Recently, several cutaneous lymphomas at the tarsal region have been reported in cats. As it differs clinically and histopathologically from the common cutaneous lymphoma, it was denominated cutaneous tarsal lymphoma. The present study describes the case of a 13-year-old male domestic longhair cat that presented with a subcutaneous mass, of 30-days evolution, at the tarsal region of the right pelvic limb. Histopathology analysis showed malignant neoplasia of round cells, morphologically suggestive of large cell lymphoma. Immunohistochemistry confirmed the diagnosis of B-immunoblastic lymphoma. Patient was treated with lomustine, and prednisolone with an overall survival time of 2.1 months. The aggressiveness of this feline lymphoma reinforces the need for further studies to understand better the disease progression and to establish improved therapeutic protocols that can increase survival time and improve quality of life of these patients.

https://doi.org/10.21897/rmvz.934 3556 downloads

12.

Caracterización isoenzimática de cultivares de nopal (Opuntia spp.)
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Flores-Hernandez, Arnoldo

; Peña-Valdivia, Cecilia B.

; Hernández-Montiel, Luis

; Ramírez-Serrano, Rogelio

; Trejo-Calzada, Ricardo

; Meza-Herrera, Cesar Alberto

; Preciado-Rangel, Pablo

; Murillo-Amador, Bernardo

Ecosistemas y recursos agropecuarios

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apr 2016, Volume 3 N. 7 Pages 75 - 89

Abstract: En Es | Text: En Es | PDF: En | PDF: Es

Taxonomic classification problems are frequent in the case of nopal (Opuntia spp.), as well as for many other genera; this complicates the accurate identification of cultivars. This study was done with the objective of detecting polymorphism in nine nopal cultivars of the 0. amyclaea Ten. (0. albicarpa), 0. megacantha, 0. crassa and 0. ficus-indica (L.) Mill, species located in the germplasm reserve of the Unidad Regional Universitaria de Zonas Aridas of the Universidad Autonoma Chapingo. The soluble protein of the roots was separated through electrophoresis to evaluate the presence of nine enzyme systems. Among these, phosphoglucomutase (PGM), 6- phosphogluconate dehydrogenase (6-PGD), glutamate oxaloacetate transaminase (GOT) and malate dehydrogenase (MDH) showed a sufficient reaction for the identification of bands of enzymatic activity. The malic enzymes (ME) and aconitase (ACO) did not present polymorphism; they are therefore not recommended for the classification of nopal cultivars. In contrast, PGM, 6-PGD, GOT and MDH presented differences or undetermined polymorphism in their band pattern; they are therefore considered relevant for the taxonomic identification and evaluation of the genetic variability of nopal.

En nopal (Opuntia spp.) como en muchos géneros, los problemas de clasificación taxonómica son frecuentes, esto complica la identificación correcta de cultivares. Este estudio se realizó con la finalidad de detectar polimorfismo en nueve cultivares de nopal de las especies O. amyclaea Ten. (O. albicarpa), O. megacantha, O. crassa y O. ficus-indica (L.) Mili., ubicados en la reserva de germoplasma de la Unidad Regional Universitaria de Zonas Áridas de la Universidad Autónoma Chapingo. La proteína soluble de las raíces se separó por electroforesis para evaluar la presencia de nueve sistemas enzimáticos. De estos, fosfoglucomutasa (PGM), 6-fosfogluconato deshidrogenasa (6-PGD), glutamato oxaloacetato transaminasa (GOT) y malato deshidrogenasa (MDH) expresaron reacción suficiente para una identificación de bandas de actividad enzimática. Las enzimas málicas (ME) y aconitasa (ACO) no presentaron polimorfismo, por tanto no se recomiendan para la clasificación de cultivares de nopal. En contraste, PGM, 6-PGD, GOT y MDH presentaron diferencias o polimorfismo indeterminado en su patrón de bandeo, por lo que se consideran relevantes para la identificación taxonómica y evaluación de la variabilidad genética de nopal.

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13.

Role of adenylyl cyclase in reduced β-adrenoceptor-mediated vasorelaxation during maturation
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López-Canales, O.A.

; Castillo-Hernandez, M.C.

; Vargas-Robles, H.

; Rios, A.

; López-Canales, J.S.

; Escalante, B.

Brazilian Journal of Medical and Biological Research

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2016, Volume 49 N. 7 elocation e5285

Abstract: En | Text: En | PDF: En

Beta-adrenergic receptor (βAR)-dependent blood vessel relaxation is impaired in older animals and G protein activation has been suggested as the causative mechanism. Here, we investigated the role of βAR subtypes (β1AR, β2AR, and β3AR) and cAMP in maturation-dependent vasorelaxation impairment. Aortic rings from 15 Sprague-Dawley male rats (3 or 9 weeks old) were harvested and left intact or denuded of the endothelium. Vascular relaxation in aortic rings from younger and older groups was compared in the presence of βAR subtype agonists and antagonists along with cAMP and cGMP antagonists. Isolated aortic rings were used to evaluate relaxation responses, protein expression was evaluated by western blot or real time PCR, and metabolites were measured by ELISA. Expression of βAR subtypes and adenylyl cyclase was assessed, and cAMP activity was measured in vascular tissue from both groups. Isoproterenol- and BRL744-dependent relaxation in aortic rings with and without endothelium from 9-week-old rats was impaired compared with younger rats. The β1AR antagonist CGP20712A (10-7 M) did not affect isoproterenol or BRL744-dependent relaxation in arteries from either group. The β2AR antagonist ICI-118,551 (10-7 M) inhibited isoproterenol-dependent aortic relaxation in both groups. The β3AR antagonist SR59230A (10-7 M) inhibited isoproterenol- and BRL744-dependent aortic ring relaxation in younger but not in older rats. All βAR subtypes were expressed in both groups, although β3AR expression was lower in the older group. Adenylyl cyclase (SQ 22536) or protein kinase A (H89) inhibitors prevented isoproterenol-induced relaxation in younger but not in older rats. Production of cAMP was reduced in the older group. Adenylyl cyclase III and RyR3 protein expression was higher in the younger group. In conclusion, altered expression of β3AR and adenylyl cyclase III may be responsible for reduced cAMP production in the older group.

https://doi.org/10.1590/1414-431x20165285 1338 downloads

14.

Efecto de la adición in situ del Pd en SBA-15 para la desoxigenación de ácido esteárico
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Pacheco-Sosa, J. G.

; Chavarria-Hernandez, J. C.

; Escobar-Morales, B.

; Ordoñez-López, L. C.

; Pérez-Vidal, H.

; Torres-Torres, J. G.

; Ferráez-Hernández, J. S.

Superficies y vacío

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2015, Volume 28 N. 3 Pages 69 - 73

Abstract: En Es | Text: En Es | PDF: En | PDF: Es

Abstract Several mesoporous SBA-15 type supports were synthesized; some of them were modified with an auxiliary organic molecule (SBA-15/TMB). The catalysts were obtained by in-situ addition of Pd (0.5- 3.0 %wt) during supports synthesis. The materials were characterized by Nitrogen physisorption, XRD, SAXS, TPR and SEM. The catalytic activity was tested in the deoxygenation of stearic acid (SA) at 300°C and 20 bar, feeding 0.25 ml/min of SA (4 %wt) in dodecane, and 50 ml/min of H2 (10%volume) in N2. The synthesized solids showed characteristic properties of mesoporous materials, and the organic molecule had an effect of expansion and disordering of the structure of the material, generating increments of the textural properties, while Pd addition did not cause significant changes in the structural properties of these materials. The catalysts showed high initial conversions of SA (40-75%), decreasing to 20-40% after 6 h reaction. Higher content of Pd produced higher conversion of SA and lower catalyst deactivation. Catalysts with TMB were less active. Selectivity to non-oxygenated products was 100% with n-heptadecane as the main product.

Resumen Se sintetizaron diferentes soportes mesoporosos tipo SBA-15, algunos fueron modificados con una molécula orgánica auxiliar (SBA-15/TMB). Los catalizadores se obtuvieron por adición in-situ de Pd (0.5-3.0% peso) durante la síntesis de los soportes. Los materiales se caracterizaron por Fisisorción de N2, DRX, SAXS, TPR y MEB. La actividad catalítica se evaluó en la desoxigenación de ácido esteárico (AE) a 300°C y 20 bar, alimentando 0.25 ml/min de AE (4%peso) en dodecano y 50 ml/min de H2 (10%volumen) en N2. Los sólidos presentaron propiedades características de materiales mesoporosos, y la molécula orgánica tuvo un efecto de ampliación y desordenamiento de la estructura del material, generando incrementos en las propiedades texturales, mientras que la adición de Pd no provocó cambios considerables en la estructura de estos materiales. Los catalizadores mostraron altas conversiones iniciales de AE (40-75%), disminuyendo la conversión a 20-40% después de 6 h de reacción. A mayor contenido de Pd se obtuvo mayor conversión de AE y menor grado de desactivación de los catalizadores. Los catalizadores con TMB fueron menos activos. La selectividad hacia productos no oxigenados fue del 100%, con n-heptadecano como producto principal.

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15.

Babesia bovis: expression of adhesion molecules in bovine umbilical endothelial cells stimulated with plasma from infected cattle
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Vargas, Marlene I.

; Patarroyo, Joaquín H.

; Hernandez, Mayra

; Peconick, Ana P.

; Patarroyo, Adriana M.

; Tafur, Gabriel A.

; Araújo, Leandro S.

; Valente, Fabrício

Pesquisa Veterinária Brasileira

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oct 2014, Volume 34 N. 10 Pages 937 - 941

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Dez bezerros machos, da raça Jersey, com 1 ano de idade com baços "in situ", sorológica e parasitologicamente livres de Babesia, foram mantidos em baias individuais no isolamento a prova de artrópodes do Depto de Veterinária desde o nascimento e ao longo de toda a experimentação. Os animais foram divididos aleatoriamente em dois grupos. Cinco animais (grupo A) foram inoculados por via intravenosa com 6,6 x10(7) hemácias parasitados com amostra patogênica de Babesia bovis (BboUFV - 1 7ª passagem) , para a determinação subseqüente "ex vivo" da expressão de moléculas de adesão . Cinco animais não inoculados (Grupo B ) foram utilizados como controlo negativo . A expressão de moléculas de adesão ICAM - 1, VCAM , PECAM - 1, E - selectina e trombospondina ( TSP ) foi medida em células endoteliais da veia umbilical de bovinos (BUVECs). As células endoteliais estimuladas com um pool de plasma proveniente de animais infectados com BboUFV - 1 7ª passagem tinham uma imunocoloração muito mais intensa de ICAM - 1 , VCAM , PECAM - 1 de E - selectina e de TSP , em comparação com as células que não receberam o estímulo . Os resultados sugerem que as citocinas pró-inflamatórias liberados na fase aguda da babesiose pode estar envolvida na expressão de moléculas de adesão , implicando , assim, elas na fisiopatologia da babesiose causada por B. bovis.

Ten male, 12-month-old Jersey with intact spleens, serologically and parasitologically free from Babesia were housed individually in an arthropod-free isolation system from birth and throughout entire experiment. The animals were randomly divided into two groups. Five animals (group A) were intravenously inoculated with 6.6 X10(7) red blood cells parasitized with pathogenic sample of Babesia bovis (passage 7 BboUFV-1), for the subsequent "ex vivo" determination of the expression of adhesion molecules. Five non-inoculated animals (group B) were used as the negative control. The expression of the adhesion molecules ICAM-1, VCAM, PECAM-1 E-selectin and thrombospondin (TSP) was measured in bovine umbilical vein endothelial cells (BUVECs). The endothelial cells stimulated with a pool of plasma from animals infected with the BboUFV-1 7th passage sample had a much more intense immunostaining of ICAM-1, VCAM, PECAM-1 E-selectin and TSP, compared to the cells which did not received the stimulus. The results suggest that proinflammatory cytokines released in the acute phase of babesiosis may be involved in the expression of adhesion molecules thereby implicating them in the pathophysiology of babesiosis caused by B. bovis.

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