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RESUMO O objetivo do estudo foi avaliar o desempenho agronômico e selecionar genótipos de sorgo biomassa para diferentes regiões do Brasil a partir da análise de adaptabilidade e estabilidade pelo método GGE Biplot. Foram avaliados 25 genótipos, que constituíram os ensaios de valor de cultivo e uso (VCU’s) de sorgo biomassa do Programa de Melhoramento da Embrapa Milho e Sorgo, conduzido em oito locais do Brasil (Sobral-CE, Jaguariúna-SP, Nova Porteirinha-MG, Planaltina-GO, Sete Lagoas-MG, Narandiba-SP, Vilhena-RO e Terra Rica-PR), na safra de 2021/2022. O delineamento experimental foi em blocos casualizados, com três repetições. Foram realizadas as análises de variância conjuntas para as características altura de plantas, florescimento e produtividades de matéria verde e seca. Constada interação (GxA), procedeu-se a análise de adaptabilidade e estabilidade pelo método de GGE Biplot, para todas as características. Com os valores de médias ajustadas, foram obtidos os agrupamentos de médias pelo teste de Scott-Knott (p < 0.05). Os genótipos de sorgo biomassa apresentam maior ciclo, bem como altura e produtividade de biomassa superior a genótipos de sorgo forrageiro. Os híbridos experimentais 202129B014 e 202129B016, bem como o híbrido comercial BRS 716 podem ser recomendados para todos os ambientes, pois apresentam alta adaptabilidade e estabilidade para todos os ambientes para a produção de matéria verde e seca. Biplot 2 VCUs VCU s (VCU’s Sorgo SobralCE, SobralCE Sobral CE, CE (Sobral-CE JaguariúnaSP, JaguariúnaSP Jaguariúna SP, SP Jaguariúna-SP PorteirinhaMG, PorteirinhaMG Porteirinha MG, MG Porteirinha-MG PlanaltinaGO, PlanaltinaGO Planaltina GO, GO Planaltina-GO LagoasMG, LagoasMG Lagoas Lagoas-MG NarandibaSP, NarandibaSP Narandiba Narandiba-SP VilhenaRO Vilhena RO RicaPR, RicaPR Rica PR , Rica-PR) 20212022 2021 2022 2021/2022 casualizados repetições plantas seca GxA, GxA (GxA) procedeuse procedeu se ajustadas ScottKnott Scott Knott p 0.05. 005 0.05 . 0 05 0.05) ciclo forrageiro B 202129B01 202129B016 71 Rica-PR 2021202 202 2021/202 (GxA 00 0.0 202129B0 7 202120 20 2021/20 0. 202129B 20212 2021/2 2021/

ABSTRACT The objective of this study was to evaluate the agronomic performance and select biomass sorghum genotypes for growing in different regions of Brazil based on adaptability and stability analysis using the GGE biplot method. The 25 genotypes evaluated were from trials of value for cultivation and use (VCU) of biomass sorghum of the Brazilian Agricultural Research Corporation (Embrapa Maize and Sorghum) Breeding Program, conducted in eight locations across Brazil (Sobral, CE; Jaguariúna and Narandiba, SP; Nova Porteirinha and Sete Lagoas, MG; Planaltina, DF; Vilhena, RO; and Terra Rica, PR) during the 2021-2022 crop season. A randomized block experimental design with three replications was used. The following traits of were subjected to joint analysis of variance: plant height, flowering, and fresh and dry matter yields. The confirmation of genotype-by-environment interaction (G×E) was followed by adaptability and stability analysis using the GGE biplot method for all traits. The adjusted means were used to obtain the mean clustering using the Scott-Knott test (p < 0.05). Biomass sorghum genotypes showed a longer growth cycle, taller plants, and higher biomass yield than forage sorghum genotypes. The experimental sorghum hybrids 202129B014 and 202129B016 and the commercial hybrid BRS 716 can be recommended for fresh and dry matter production in all tested environments due to their high adaptability and stability. 2 VCU (VCU Embrapa Sorghum Program Sobral, Sobral (Sobral CE Narandiba SP Lagoas MG Planaltina DF Vilhena RO Rica PR 20212022 2021 2022 2021-202 season variance height flowering yields genotypebyenvironment genotype environment G×E GE G E (G×E ScottKnott Scott Knott p 0.05. 005 0.05 . 0 05 0.05) cycle plants B 202129B01 71 2021202 202 2021-20 00 0.0 202129B0 7 202120 20 2021-2 0. 202129B 20212 2021-

https://doi.org/10.1590/1983-21252025v3812509rc

Radiosensibilidad de tres variedades de tomate (Solanum lycopersicum L.) irradiadas con rayos gamma de Cobalto-60
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Jaén Villarreal, Jorge Enrique

; González Cepero, María Caridad

; Camargo Buitrago, Ismael

; Saéz Cigarruista, Ana Elida

; Guillama Alonso, Rodolfo

; Guerra Murillo, José Ángel

Agronomía Mesoamericana

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dec 2024, Volume 35 N. 1 elocation 54329

Abstract: Es En | Text: Es En | PDF: Es | PDF: En

Resumen Introducción. El tomate (Solanum lycopersicum L.) es considerado uno de los cultivos más importantes en el mundo. La mejora genética del tomate ha empleado diferentes métodos para generar variabilidad, la inducción de mutaciones es una de las técnicas empleadas en la obtención de variedades mejoradas. El primer paso, en el uso de esta herramienta, es el estudio de radiosensibilidad para determinar la dosis de irradiación a emplear. Objetivo. Determinar la radiosensibilidad y la dosis de rayos gamma de cobalto-60 a emplear en tres variedades de tomate. Materiales y métodos. Esta investigación se desarrolló en enero del año 2020, en el Instituto Nacional de Ciencias Agrícolas de Cuba. Se irradiaron con rayos gamma de Cobalto-60 semillas de tres variedades de tomate, con dosis entre 100 Gy y 900 Gy, con intervalos de dosis de 100 Gy; también se empleó un control sin irradiar. Se evaluaron el porcentaje de germinación, la altura y la sobrevivencia de las plántulas. Resultados. Se observó que a mayores dosis de irradiación se redujo la germinación, la sobrevivencia y la altura de las plántulas. Las variedades IDIAP T-7 e IDIAP T-9 redujeron su germinación a la dosis de 400 Gy y el cultivar DINA RPs a los 300 Gy. Los genotipos presentaron valores de sobrevivencia por debajo del 50 % a la dosis de 800 Gy. La reducción de altura inició a la dosis de 200 Gy para la variedad IDIAP T-7, 300 Gy para DINA RPs y 400 Gy para IDIAP T-9. Conclusión. Las dosis de DL-50 identificadas fueron 692, 588 y 630 Gy y las dosis deGR-50 fueron 407, 467 y 380 Gy para las variedades IDIAP T-7, IDIAP T-9 Y DINA RPs, respectivamente. Se seleccionó la dosis GR-50 de cada una de las variedades para el programa de mejora por mutaciones.

Abstract Introduction. Tomato (Solanum lycopersicum L.) is considered one of the most important crops in the world. Genetic improvement of tomatoes has employed different methods to generate variability, and mutation the induction of mutations is one of the techniques used to obtaining improved varieties. The first step in using this tool is studying radiosensitivity to determine the irradiation dose to be used. Objective. To determine the radiosensitivity and the dose of cobalt-60 gamma rays to be used in three tomato varieties. Materials and methods. This research was conducted in January 2020 at the Instituto Nacional de Ciencias Agrícolas of Cuba. Seeds of three tomato varieties were irradiated with cobalt-60 gamma rays, with doses ranging from 100 Gy to 900 Gy, at intervals of 100 Gy; an unirradiated control was also used. The percentage of germination, plant height, and seedling survival were evaluated. Results. It was observed that higher irradiation dose reduced germination, survival, and plant height. The IDIAP T-7 and IDIAP T-9 varieties reduced germination at a dose of 400 Gy, while the DINA RPs cultivar reduced germination at 300 Gy. Genotypes showed survival values below 50 % at a dose of 800 Gy. Height reduction began at doses of 200 Gy for IDIAP T-7, 300 Gy for DINA RPs, and 400 Gy for IDIAP T-9. Conclusion. The DL-50 doses were identified as 692, 588, and 630 Gy, and the GR-50 doses were 407, 467, and 380 Gy for the IDIAP T-7, IDIAP T-9, and DINA RPs varieties, respectively. The GR-50 dose of each variety was selected for the mutation breeding program.

https://doi.org/10.15517/am.2024.54329

First heart transplant at the General Hospital of Mexico "Dr. Eduardo Liceaga"
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Castaño-Guerra, Rodolfo de J.

; Borges-López, Jesús S.

; Ramírez-Castañeda, Serafín

; Ayala-Hernández, Eduardo

; Barón-Caballero, José L.

; Guerrero-Avendaño, Guadalupe M. L.

Revista médica del Hospital General de México

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sep 2023, Volume 86 N. 3 Pages 115 - 120

Abstract: En | Text: En | PDF: En

Abstract Nowadays, cardiac transplant is considered the most efficient treatment to better the prognosis in patients with last stage cardiac insufficiency that is impossible to obtain through regular medical and/or surgical treatments. We report the case of the first cardiac transplant performed at the General Hospital of Mexico "Dr. Eduardo Liceaga," we describe the patient's and donor's characteristics, pre- and post-surgical management, immunosuppressive therapy, cardiac rehabilitation, and patient follow-up. As well as the multidisciplinary approach necessary to achieve a successful result.

https://doi.org/10.24875/hgmx.22000069

Coartación aórtica: una causa infradiagnosticada de hipertensión arterial secundaria
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Borges-López, Jesús S.

; Castaño-Guerra, Rodolfo de J.

; Ayala-Hernández, Eduardo

; Rosas-Munive, Emma

; Barón-Caballero, José L.

; Borges-López, Luis M.

Revista mexicana de medicina familiar

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dec 2022, Volume 9 N. 4 Pages 116 - 121

Abstract: Es En | Text: Es En | PDF: Es | PDF: En

RESUMEN La coartación aórtica representa el 5-8% de todas las cardiopatías congénitas y se define como un estrechamiento de la luz aórtica a nivel del ligamento arterioso o debajo de este, clasificándose en preductal o posductal, y existen varias teorías que tratan de explicar su etiología, que aún sigue siendo poco clara. Puede diagnosticarse desde la infancia o en la edad adulta, debe sospecharse en todo paciente antes de los 30 años de edad que presente hipertensión arterial sistémica para poder brindar un tratamiento quirúrgico oportuno.

ABSTRACT Aortic coarctation represents 5-8% of all congenital heart diseases and is defined as a narrowing of the aortic lumen at or below the ligamentum arteriosum, classified as preductal or postductal, and there are several theories that try to explain its etiology, which is still unclear. It can be diagnosed from childhood or in adulthood, and every patient before 30 years old, should be suspected of having systemic arterial hypertension, in order to provide timely surgical treatment.

https://doi.org/10.24875/rmf.22000011

Guía de práctica clínica mexicana para el diagnóstico y tratamiento de las dislipidemias y enfermedad cardiovascular aterosclerótica
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Pavía-López, Abel A.

; Alcocer-Gamba, Marco A.

; Ruiz-Gastelum, Edith D.

; Mayorga-Butrón, José L.

; Mehta, Roopa

; Díaz-Aragón, Filiberto A.

; Aldrete-Velasco, Jorge A.

; López-Juárez, Nitzia

; Cruz-Bautista, Ivette

; Chávez-Mendoza, Adolfo

; Secchi-Nicolás, Nikos C.

; Guerrero-Martínez, Francisco J.

; Cossio-Aranda, Jorge E.

; Mendoza-Zubieta, Victoria

; Fanghänel-Salmon, Guillermo

; Valdivia-Proa, Martha

; Olmos-Domínguez, Luis

; Aguilar-Salinas, Carlos A.

; Dávila-Maldonado, Luis

; Vázquez-Rangel, Armando

; Pavia-Aubry, Vanina

; Nava-Hernández, María de los A.

; Hinojosa-Becerril, Carlos A.

; Anda-Garay, Juan C.

; Ríos-Ibarra, Manuel O. de los

; Berni-Betancourt, Ana C.

; López-Cuellar, Julio

; Araiza-Garaygordobil, Diego

; Rivera-Reyes, Romina

; Borrayo-Sánchez, Gabriela

; Tapia-Hernández, Mónica

; Cano-Nigenda, Claudia V.

; Guerra-López, Arturo

; Elías-López, Josué

; Figueroa-Morales, Marco A.

; Montaño-Velázquez, Bertha B.

; Velasco-Hidalgo, Liliana

; Rodríguez-Lozano, Ana L.

; Pimentel-Hernández, Claudia

; Baquero-Hoyos, María M.

; Romero-Moreno, Felipe

; Rodríguez-Vega, Mario

Archivos de cardiología de México

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2022, Volume 92 Suppl. 1 Pages 1 - 62

Abstract: Es En | Text: Es En | PDF: Es | PDF: En

resumen está disponible en el texto completo

Abstract Background: Cardiovascular diseases are the leading cause of mortality worldwide and Mexico is no exception. The epidemiological data obtained in 1990 showed that cardiovascular diseases represented 19.8% of all causes of death in our country. This figure increased significantly to 25.5% for 2015. Some national surveys suggest that more than 60% of the adult population has at least one risk factor for cardiovascular disease (obesity or overweight, hypertension, smoking, diabetes, dyslipidemias). On the other hand, data from the Pan American Health Organization have linked the process of atherosclerosis as the first cause of premature death, significantly reducing life expectancy, which has enormous social repercussions. Objective: This document constitutes the Clinical Practice Guide (CPG) prepared at the initiative of the Mexican Society of Cardiology in collaboration with the Mexican Society of Nutrition and Endocrinology, AC, National Association of Cardiologists of Mexico, AC, Mexican Association for the Prevention of Atherosclerosis and its Complications, AC, National Normative Committee of General Medicine, AC, National College of Geriatric Medicine, AC, College of Internal Medicine of Mexico, AC, Mexican Society of Angiology and Vascular and Endovenous Surgery, AC, Mexican Institute of Research Nephrological, AC and the Mexican Academy of Neurology, A.C.; with the methodological support of the Ibero-American Agency for the Development and Evaluation of Health Technologies, in order to establish recommendations based on the best available evidence and agreed upon by an interdisciplinary group of experts. The objective of this document is to provide evidence-based recommendations to help decision makers in the diagnosis and treatment of dyslipidemias in our country. Material and methods: This document complies with international quality standards, such as those described by the Institute of Medicine of the USA, the Institute of Clinical Excellence of Great Britain, the Scottish Intercollegiate Guideline Network and the Guidelines International Network. A multidisciplinary group of clinical experts and methodologists with experience in systematic reviews of the literature and the development of clinical practice guidelines was formed. A scope document was agreed upon, relevant clinical questions were established, the best available evidence critically evaluated in systematic literature reviews was exhaustively identified, and clinical recommendations were developed. The modified Delphi Panel methodology was used to achieve an adequate level of consensus in each of the recommendations contained in this CPG. Results: 23 clinical questions were agreed upon which gave rise to their respective clinical recommendations. Conclusions: We consider that this document contributes to better clinical decision-making and becomes a point of reference for clinicians and patients in the management of dyslipidemias and this contributes to reducing the morbidity and mortality derived from atherosclerotic cardiovascular events in our country.

https://doi.org/10.24875/acm.m22000081 1458 downloads

Consenso de leucemia mieloide aguda en México
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Arana-Luna, Luara L.

; Alvarado-Ibarra, Martha

; Silva-Michel, Luis G.

; Morales-Maravilla, Adrián

; González-Rubio, María del C.

; Chávez-Aguilar, Lénica A.

; Tena-Iturralde, María Fernanda

; Mojica-Balceras, Liliana

; Zapata-Canto, Nidia

; Galindo-Delgado, Patricia

; Miranda-Madrazo, María Raquel

; Morales-Hernández, Alba E.

; Silva-Vera, Karina

; Grimaldo-Gómez, Flavio A.

; Hernández-Caballero, Álvaro

; Bates-Martin, Ramón A.

; Álvarez-Vera, José L.

; Tepepa-Flores, Fredy

; Teomitzi-Sánchez, Óscar

; Fermín-Caminero, Denisse J.

; Peña-Celaya, José A. de la

; Salazar-Ramírez, Óscar

; Flores-Villegas, Luz V.

; Guerra-Alarcón, Lidia V.

; Leyto-Cruz, Faustino

; Inclán-Alarcón, Sergio I.

; Milán-Salvatierra, Andrea I.

; Ventura-Enríquez, Yanet

; Pérez-Lozano, Uendy

; Báez-Islas, Pamela E.

; Tapia-Enríquez, Ana L.

; Palma-Moreno, Orlando G.

; Aguilar-Luévano, Jocelyn

; Espinosa-Partida, Arturo

; Pérez-Jacobo, Luis F.

; Rojas-Castillejos, Flavio

; Ruiz-Contreras, Josué I.

; Loera-Fragoso, Sergio J.

; Medina-Coral, Jesús E.

; Acosta-Maldonado, Brenda L.

; Soriano-Mercedes, Emely J.

; Saucedo-Montes, Erick E.

; Valero-Saldana, Luis M.

; González-Prieto, Susana G.

; Nava-Villegas, Lorena

; Hernández-Colin, Ana K.

; Hernández-Alcántara, Areli E.

; Zárate-Rodríguez, Pedro A.

; Ignacio-Ibarra, Gregorio

; Meillón-García, Luis A.

; Espinosa-Bautista, Karla A.

; Ledesma de la Cruz, Cindy

; Barbosa-Loría, Diego M.

; García-Castillo, Carolina

; Balderas-Delgado, Carolina

; Cabrera-García, Álvaro

; Pérez-Zúñiga, Juan M.

; Hernández-Ruiz, Eleazar

; Villela-Peña, Atenas

; Gómez Cortés, Sue Cynthia

; Romero-Rodelo, Hilda

; Garzón-Velásquez, Katheryn B.

; Serrano-Hernández, Cristina

; Martínez-Ríos, Annel

; Pedraza-Solís, María Luisa

; Martínez-Coronel, Jorge A.

; Narváez-Davalos, Iris M.

; García-Camacho, Alinka S.

; Merino-Pasaye, Laura E.

; Aguilar-Andrade, Carolina

; Aguirre-Domínguez, Juan A.

; Guzmán-Mera, Pedro G.

; Delgado-de la Rosa, Elizabeth

; Flores López, Perla E.

; González-Aguirre, Lilia L.

; Ramírez-Alfaro, Edgar M.

; Vera-Calderón, Heidi

; Meza-Dávalos, María Lizeth

; Murillo-Cruz, Juan

; Pichardo-Cepín, Yayra M.

; Ramírez-Romero, Eva F.

Gaceta médica de México

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2022, Volume 158 N. spe3 Pages M1 - M51

Abstract: En Es | Text: En Es | PDF: En | PDF: Es

Abstract Acute myeloid leukemia (AML) comprises a heterogeneous group of hematopoietic cell neoplasms of myeloid lineage that arise from the clonal expansion of their precursors in the bone marrow, interfering with cell differentiation, leading to a syndrome of bone marrow failure. AML is a consequence of genetic and epigenetic changes (point mutations, gene rearrangements, deletions, amplifications, and arrangements in epigenetic changes that influence gene expression) in hematopoietic precursor cells, which create a clone of abnormal cells that are capable of proliferating but cannot differentiate into mature hematopoietic cells or undergo programmed cell death. The diagnosis requires more than 20% myeloid blasts in the bone marrow and certain cytogenic abnormalities. Treatment will depend on age, comorbidities, and cytogenetic risk among the most frequent.

resumen está disponible en el texto completo

https://doi.org/10.24875/gmm.m21000597 895 downloads

Forced Degradation Study and Development of a Stability-Indicating Method for Amoxicillin Capsules According to ANVISA Guidelines
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Gallo, Margareth B. C

; Nascimento, Diogo D. do

; Nunes, Nelson M

; Sousa, Flávia F. M. de

; Boniatti, Janine

; Aguiar, José L. N. de

; Medeiros, Juliana J. S

; Esteves, Alessandra L

; Guerra, Graça M. S

; Ferreira, Luiz E. M

; Regis, Lucas G. I

; Seiceira, Rafael C

; Moncorvo, Fabiana M. S. U

; Chaves, Marcelo H. C

Journal of the Brazilian Chemical Society

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dec 2021, Volume 32 N. 12 Pages 2195 - 2205

Abstract: En | Text: En | PDF: En

This study aimed to develop a reliable stability-indicating method (SIM) for amoxicillin 500 mg capsules (DP-drug product). A literature review addressing amoxicillin (AMX; DS-drug substance) forced degradation studies and the existing SIMs was conducted to verify the most significant outcomes. Subsequently, the forced degradation of DP and DS was carried out following the Brazilian Health Surveillance Agency (ANVISA) guidelines, including thermal degradation (dry and wet heat), acidic and alkaline hydrolyses, hydrogen peroxide oxidation, reaction with copper, and photodegradation. Both DS and DP were more susceptible to 0.015 M NaOH, resulting in approximately 50% degradation. AMX DS and DP were not significantly photodegraded, but some degradation products (PDegs) showed susceptibility to light exposure. Thermodegraded samples showing ≥ 10% degradation exhibited modified profiles in thermogravimetric (TG) and differential scanning calorimetric (DSC) analyses. The X-ray powder diffraction patterns (XRPD) of DS samples exposed to dry and wet heat displayed complete amorphization of AMX, attesting to the occurrence of physical degradation concomitantly with chemical degradation, which can alter the drug’s bioavailability. In contrast, the thermodegraded DP samples exhibited intact AMX crystals interspersed with the amorphous form, perhaps partly protected by the excipient. The validated SIM was able to detect and quantify about 80 PDegs.

https://doi.org/10.21577/0103-5053.20210111

Tratamiento de lesiones de rodilla mediante prototipo robot controlado por aplicaciones móviles
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Rea Yánez, Mercy Dalila

; Guerra Salazar, José E.

; Barrazueta R, Sandra G.

; Morales Gordon, Jose L.

Revista Científica y Tecnológica UPSE (RCTU)

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dec 2021, Volume 8 N. 2 Pages 12 - 26

Abstract: Es En | Text: Es En | PDF: Es | PDF: En

Resumen Los prototipos electrónicos desarrollados en los últimos tiempos se están utilizando en el campo de la medicina y la rehabilitación física, bajo esta premisa se desarrolló un tratamiento para lesiones de rodilla mediante un prototipo robot controlado por aplicaciones móviles, el diseño y construcción se realizó en tres etapas: diseño mecánico, electrónico y software. La estructura mecánica se realizó a través de un sistema de transmisión por medio de un tornillo de potencia, garantizando la transformación del movimiento giratorio generado por un motor a un movimiento lineal asegurando un desplazamiento de 120° en flexión a 0° en extensión necesaria para la rehabilitación. En la segunda etapa se alimentó desde una fuente de 12V, controlado mediante un Arduino MEGA 2560 el cual recibió los datos enviados por Bluetooth, desde un encoder rotatorio y dos finales de carrera. La tercera etapa constó de dos modos de operación, una manual que permite variar la velocidad y el segundo que ejecuta rutinas preestablecidas controladas de forma inalámbrica a distancias de hasta 7.5m por medio de una aplicación móvil que dispone de una base de datos local que almacena los datos del paciente. Se comprobó que el prototipo en operación con y sin paciente presenta un ángulo error de 2°, que según expertos no incide en los resultados de la rehabilitación de los pacientes, lo que le hace un equipo fiable en procesos de terapia.

Abstract The electronic prototypes developed in recent times are being used in the field of medicine and physical rehabilitation under this premise, a treatment for knee injuries was developed by means of a robot prototype controlled by mobile applications, the design and construction was carried out in three stages: mechanical, electronic and software design. The mechanical structure was made through a transmission system by means of a power screw, ensuring the transformation of the rotary motion generated by a motor to a linear motion ensuring a displacement of 120° in flexion to 0° in extension necessary for rehabilitation. The second stage was powered from a 12V source, controlled by an Arduino MEGA 2560 which received the data sent by Bluetooth, from a rotary encoder and two limit switches. The third stage consisted of two modes of operation, a manual one that allows to vary the speed and the second one that executes pre-set routines controlled wirelessly at distances of up to 7.5m by means of a mobile application that has a local database that stores the patient's data. It was verified that the prototype in operation with and without patient presents an error angle of 2°, which according to experts does not affect the rehabilitation results of the patients, making it a reliable equipment in therapy processes.

https://doi.org/10.26423/rctu.v8i2.576

Efectos de la inyección neonatal de estreptozotocina sobre indicadores bioquímicos y de estrés oxidativo en ratas
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Bequer, Leticia

; Quintero, Maryoil

; Gómez, Tahiry

; Guerra, Isbey

; Molina, José L.

; Freire, Cindy

; Clapés, Sonia

Revista Cubana de Investigaciones Biomédicas

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sep 2021, Volume 40 N. 3 elocation e804

Abstract: En Es | Text: En Es | PDF: En | PDF: Es

ABSTRACT Introduction: Streptozotocin is currently one of the most used diabetogenic agents to generate biological models of diabetes due to its chemical properties, so it is necessary to study the consequences of STZ for the organism of the laboratory animal. Objective: To evaluate in a period of 90 days the effects of neonatal injection of streptozotocin in Wistar rats on biochemical indicators and oxidative stress in liver and kidney. Methods: Diabetes was induced neonatally by 100 mg of streptozotocin in Wistar rats. Blood glucose, insulin and oxidative stress indicators in liver and kidney were determined in 5 animals per group at days 5, 10, 20, 30, 60, 90 of birth. Results: Blood glucose and insulin showed significant differences in the STZ-group respect to the control group in all interventions. The maximum value of hyperglycemia was observed on day-5. The concentration of nitrates and nitrites in liver was higher than in kidney. In liver tissue of the STZ-group, this indicator was significantly higher on days 10-20 compared to the control. In all interventions, reduced glutathione consumption was demonstrated in the STZ-group compared to control in both organs. In the liver of STZ rats no lipid or protein damage was demonstrated. However, in the kidney, significant damage in both biomolecules was detected in the STZ-group on day-5. Conclusions: Neonatal streptozotocin cytotoxicity as well as induced glucose and insulin concentrations had a negative impact on oxidative stress indicators studied in liver and kidney tissue.

RESUMEN Introducción: Debido a sus propiedades químicas, la estreptozotocina es uno de los agentes diabetogénicos más utilizados para generar modelos biológicos de diabetes, por lo que es necesario estudiar cuáles son sus efectos en el organismo del animal de laboratorio. Objetivo: Evaluar, en un periodo de 90 días, los efectos de la inyección neonatal de estreptozotocina en ratas Wistar sobre indicadores bioquímicos y de estrés oxidativo en hígado y riñón. Métodos: La diabetes fue inducida neonatalmente por 100 mg de estreptozotocina en ratas Wistar. Se realizaron determinaciones de glucemia, insulina e indicadores de estrés oxidativos en hígado y riñón en cinco animales por grupo a los días 5, 10, 20, 30, 60, 90 de nacidos. Resultados: En todas las intervenciones, la glucemia e insulina mostraron diferencias significativas en el grupo-STZ respecto al control. El valor máximo de hiperglucemia se observó al quinto día. La concentración de nitratos y nitritos en hígado fue mayor que en riñón. En comparación con el grupo control, en el tejido hepático del grupo-STZ la concentración de nitratos y nitritos resultó significativamente superior los días 10-20. En todas las intervenciones se detectó consumo de glutatión reducido en ambos órganos. En el hígado de las ratas STZ no se demostró daño a lípidos ni proteínas; sin embargo, en riñón se detectó daño significativo en ambas biomoléculas al quinto día. Conclusiones: Tanto la citotoxicidad de la estreptozotocina neonatal como las concentraciones de glucosa e insulina inducidas repercutieron negativamente sobre los indicadores de estrés oxidativo estudiados en tejido hepático y renal.

10.

Implementación de una WSN para georreferenciación y generación de alertas de personas en zonas sin cobertura celular
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Robalino Espinoza, Viviana

; Tamayo Freire, Alexis

; Morales, José L.

; Guerra Salazar, José

; Zúñiga, Wilson A.

Revista Científica y Tecnológica UPSE (RCTU)

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jun 2021, Volume 8 N. 1 Pages 32 - 41

Abstract: Es En | Text: Es En | PDF: Es | PDF: En

Resumen Se realizó el diseño y construcción de un prototipo de comunicación inalámbrica para la georreferenciación y generación de alertas, compuesto por: Nodo Sensor, Nodo Router y Nodo Coordinador. La red fue estructurada con la topología tipo malla, que permite obtener la información de georreferenciación, velocidad, alturas obtenidas del GPS y las alertas generadas por el usuario. Se desarrolló una aplicación en el paquete Visual Studio Community que permite al administrador ingresar la información de los portadores a una base de datos al igual que el recorrido de manera automática, mostrándolo en un mapa georreferenciado, la interfaz gráfica permite la búsqueda de la información almacenada y su exportación. Se demostró que el prototipo implementado puede cubrir 500m2 en terreno montañoso y 4500m2 en terreno plano con una potencia de recepción entre -72.3 dBm y -75.9 dBm mostrando un margen de recepción entre 28.7 dB a 25.1 dB. La red tiene una capacidad de 128 dispositivos cubriendo el 213% de la capacidad actual requerida. El dispositivo GPS presenta un error de ubicación de ± 2.5 metros, de velocidad de ± 0.1 m/s y de altura de ± 20 metros demostrando que el prototipo no incorpora error a los sensores. La autonomía energética es de 16 horas cubriendo el 400% del tiempo normal del recorrido de un visitante. Se concluye que el prototipo implementado puede convertirse en una herramienta de ayuda para facilitar la ubicación de personas que requieran auxilio dentro de la Reserva cumpliendo así los objetivos nacionales sobre seguridad.

Abstract The design and construction of a wireless communication prototype for georeferencing and alert generation, composed of: Sensor Node, Router Node and Coordinator Node. The network was structured with a mesh type topology, which allows obtaining georeferencing information, speed, height obtained from the GPS and the alerts generated by the user. An application was developed in the Visual Studio Community package that allows the administrator to enter the information of the carriers to a database as well as the route automatically, showing it on a georeferenced map, the graphical interface allows the search of the stored information and its export. It was demonstrated that the implemented prototype can cover 500m2 in mountainous terrain and 4500m2 in flat terrain with a reception power between -72.3 dBm and -75.9 dBm showing a reception margin between 28.7 dB to 25.1 dB. The network has a capacity of 128 devices covering 213% of the current required capacity. The GPS device has a location error of ± 2.5 meters, speed error of ± 0.1 m/s and height error of ± 20 meters demonstrating that the prototype does not incorporate sensor error. The energy autonomy is 16 hours, covering 400% of the normal travel time of a visitor. It is concluded that the implemented prototype can become a tool to help locate people in need of assistance within the reserve, thus meeting national security objectives.

https://doi.org/10.26423/rctu.v8i1.556

11.

Reactividad de sueros de ratones inmunizados con vesículas de membrana externa derivadas de Salmonella enterica
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Guerra-Peña, Yuneisy

; Remedios-Jiménez, Laura

; Izquierdo-Fiallo, Katherin

; Gonzales-Aznar, Elizabeth

; Fajardo-Sánchez, Abel

; Fernández-Castillo, Sonsire

; García-Rivera, Dagmar

; Pérez-Quiñoy, José L.

Vaccimonitor

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apr 2021, Volume 30 N. 1 Pages 33 - 38

Abstract: En Es | Text: En Es | PDF: En | PDF: Es

ABSTRACT Typhoid fever caused by Salmonella Paratyphi A (paratyphoid fever) is indistinguishable from that caused by Salmonella Typhi and the degree of incidence has increased in recent years, especially in Southeast Asia. On the other hand, diarrhea and other enteric complications caused by Salmonella Enteritidis and Salmonella Typhimurium continue to be a serious health problem, especially in underdeveloped countries. Vaccines continue to be the most effective way to prevent these diseases. There are vaccines based on Salmonella Typhi capsular polysaccharide, which protects against typhoid fever; however, there are no effective vaccines licensed for use in humans to prevent disease caused by nontyphoidal Salmonella serotypes. Developing a formulation capable of protecting against these diseases remains a challenge for the scientific community. In this work, the reactivity of the sera of mice immunized subcutaneously with formulations based on Outer Membrane Vesicles (OMV) derived from Salmonella Paratyphi A, Salmonella Enteritidis and Salmonella Typhimurium, was evaluated by Western blot, against the respective cell lysates to identify the formulation that induces the best cross immune response. The results obtained indicated a high reactivity of all the sera to the lysates; without an apparent difference between them. Undoubtedly, immunogenicity tests followed by cross-challenge tests should be performed to identify a vaccine candidate. These results suggest that the OMV used in this study are composed of possibly conserved antigens in the three Salmonella serotypes and that they can induce a broad-spectrum immune response and cross protection.

RESUMEN La fiebre tifoidea causada por Salmonella Paratyphi A (fiebre paratifoidea) es indistinguible de la producida por Salmonella Typhi y el grado de incidencia ha aumentado en los últimos años, especialmente en el sudeste asiático. Por otro lado, la diarrea y otras complicaciones entéricas causadas por Salmonella Enteritidis y Salmonella Typhimurium continúan siendo un problema de salud grave, especialmente en países subdesarrollados. Las vacunas continúan siendo la forma más efectiva de prevenir estas enfermedades. Existen vacunas basadas en el polisacárido capsular de Salmonella Typhi que protegen contra la fiebre tifoidea; sin embargo, no hay vacunas efectivas licenciadas para uso en humanos que prevengan las enfermedades producidas por los serotipos de Salmonella no tifoideas. El desarrollo de una formulación con capacidad para proteger contra estas enfermedades sigue siendo un desafío para la comunidad científica. En este trabajo se evaluó, mediante Western blot, la reactividad de los sueros de ratones inmunizados por vía subcutánea con formulaciones basadas en vesículas de membrana externa derivadas de Salmonella Paratyphi A, Salmonella Enteritidis y Salmonella Typhimurium, contra los respectivos lisados celulares, para identificar la formulación que induce la mejor respuesta inmunológica cruzada. Los resultados obtenidos indicaron una alta reactividad de todos los sueros a los lisados, sin una diferencia aparente entre ellos. Sin lugar a dudas, se deberán realizar pruebas de inmunogenicidad seguidas de pruebas de retos cruzados para identificar un candidato vacunal. Estos resultados sugieren que las vesículas de membrana externa empleadas en este estudio están compuestas por antígenos posiblemente conservados en los tres serotipos de Salmonella y que pueden inducir una respuesta inmune de amplio espectro y protección cruzada.

12.

Consenso mexicano de linfoma de Hodgkin
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Álvarez-Vera, José L.

; Aguilar-Luevano, Jocelyn

; Alcívar-Cedeño, Luisa M.

; Arana-Luna, Luara L.

; Arteaga-Ortiz, Luis

; Báez-Islas, Pamela E.

; Carolina-Reynoso, Ana

; Cesarman-Maus, Gabriela

; Peña-Celaya, José A. De la

; Espitia-Ríos, María E.

; Fermín-Caminero, Denisse J.

; Flores-Patricio, Willy

; García-Camacho, Alinka S.

; Guzmán-Mora, Pedro G.

; Hernández-Colín, Ana K.

; Hernández-Ruiz, Eleazar

; Herrera-Olivares, Wilfrido

; Jacobo-Medrano, Elvia

; Loera-Fragoso, Sergio J.

; Macías-Flores, Juan P.

; Martínez-Ramírez, Mario A.

; Medina-Guzmán, Liliana

; Milán-Salvatierra, Andrea I.

; Montoya-Jiménez, Leire

; Morales-Adrián, Javier de J.

; Mujica-Martínez, Aldo

; Nava-Villegas, Lorena

; Orellana-Garibay, Juan J.

; Palma-Moreno, Orlando G.

; Pérez-Zúñiga, Juan M.

; Pérez-Gómez, Karen D.

; Pichardo-Cepín, Yayra M.

; Rojas-Castillejos, Flavio

; Romero-Martínez, Eduardo

; Romero-Rodelo, Hilda

; Segura-García, Adela

; Silva-Vera, Karina

; Tapía-Enríquez, Ana L.

; Teomitzi-Sánchez, Óscar

; Tepepa-Flores, Fredy

; Vilchis-González, Shendel P.

; Villela-Peña, Atenas

; Guerra-Alarcón, Lidia V.

; Reséndiz-Olea, Rodrigo

; Banda-García, Luisa

; Paredes-Lozano, Eugenia P.

; Alvarado-Ibarra, Martha

Gaceta médica de México

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2021, Volume 157 Suppl. 2 Pages S1 - S18

Abstract: En Es | Text: En Es | PDF: En | PDF: Es

Abstract Hodgkin’s lymphoma is due to the clonal transformation of cells originating from B lymphocytes, generating the pathognomonic binucleate Reed-Sternberg cells. Hodgkin’s lymphoma is a B cell disease with a bimodal distribution, with higher incidence in adolescence and the third decade of life, showing a second peak in people over 55 years of age. Classic Hodgkin lymphoma cells routinely undergo gene expression reprogramming, as they lose the expression of most of the typical B-cell genes and acquire the expression of multiple genes that are typical of other types of cells in the immune system. The treatment algorithm will depend on whether it is classic or predominantly lymphocytic HL, if it is early stage with unfavorable prognostic markers or not, the initial management regimen, and whether there is bulky disease, among the most relevant variables.

Resumen El linfoma de Hodgkin (LH) se debe a la transformación clonal de células originadas en los linfocitos B, lo que genera las células binucleadas patognomónicas de Reed-Sternberg. El LH es una enfermedad de células B con una distribución bimodal, con mayor incidencia en la adolescencia y la tercera década de la vida y un segundo pico en personas mayores de 55 años. Las células del LH clásico habitualmente sufren una reprogramación de la expresión génica, ya que pierden la expresión de la mayoría de los genes típicos de las células B y han adquirido la expresión de múltiples genes que son típicos de otros tipos de células del sistema inmunitario. El algoritmo de tratamiento dependerá si se trata de LH clásico o de predominio linfocítico, si es un estadio temprano con marcadores de pronóstico desfavorables o no, el esquema inicial de manejo y si existe enfermedad voluminosa, entre las variables más relevantes.

https://doi.org/10.24875/gmm.m21000500 304 downloads

13.

EFFECTS OF SOIL WATER AVAILABILITY AND ORGANIC MATTER CONTENT ON FRUIT YIELD AND SEED OIL CONTENT OF CASTOR BEAN1
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Lacerda, Rogério D. de

; Almeida, Larissa C.

; Guerra, Hugo O. C.

; Silva, José E. B. da

Engenharia Agrícola

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dec 2020, Volume 40 N. 6 Pages 703 - 710

Abstract: En | Text: En | PDF: En

ABSTRACT The objective of this study was to assess the sensitivity of the castor bean cultivar “BRS 188 Paraguaçu” to available soil water for plants and soil organic matter content based on their effects on fruit yield and seed oil content. A 2 × 4 factorial experiment was conducted from October 2008 to March 2009 under field conditions using randomized complete block design; two soil organic matter contents (5.0 and 25.0 g·kg−1) and four available soil water levels for plants at the irrigation time (70, 80, 90, and 100% of the total available soil water) with three replicates were tested. On each experimental unit measuring 100 m2, 50 plants were cultivated with a spacing of 2 m × 1 m (24 useful and 26 border plants). Analysis of variance (F test) was performed; qualitative factors (soil organic matter) were compared by Tukey's test and quantitative factors (available soil water) by regression analysis. Increased soil organic matter and available soil water for plants substantially increased productivity; however, seed oil content, mean 100-seed mass, mean 100-fruit mass, and seed:fruit ratio were affected by available soil water content alone. Therefore, the tested cultivar is sensitive to reduced available soil water content, which severely decreases its productivity.

https://doi.org/10.1590/1809-4430-eng.agric.v40n6p703-710/2020 468 downloads

14.

Recomendaciones de expertos para el tratamiento biológico en pacientes con psoriasis
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Podoswa-Ozerkovsky, Nancy

; Amaya-Guerra, Mario

; Barba-Gómez, José F.

; Estrada-Aguilar, Lorena

; Gómez-Flores, Minerva

; Lopeztello-Santillán, Adriana L.

; Maldonado-García, César A.

; Rivera-Gómez, Mónica I.

; Villanueva-Quintero, Delfina G.

; León-Dorantes, Gladys

Gaceta médica de México

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oct 2020, Volume 156 N. 5 Pages 454 - 462

Abstract: En Es | Text: En Es | PDF: En | PDF: Es

Abstract In recent years, the introduction of a series of biological drugs for the treatment of psoriasis has considerably increased the therapeutic armamentarium of doctors, and thus a strongly positive impact on the control of this condition has been achieved. With the purpose to provide the best recommendations for the use of these biological agents in patients with psoriasis, the Mexican group of psoriasis experts, PSOMEX, has developed recommendations in order to improve the understanding and therapeutic positioning of this type of medications.

Resumen En los últimos años, la introducción de diversos medicamentos biológicos para el tratamiento de la psoriasis ha aumentado considerablemente el arsenal terapéutico del médico, con lo cual se ha logrado un fuerte impacto positivo en el control de la enfermedad. Con el fin de proveer de las mejores recomendaciones para el uso de estos biológicos en los pacientes afectados de psoriasis, el grupo mexicano de expertos en psoriasis PSOMEX ha formulado recomendaciones para mejorar la comprensión y el posicionamiento terapéutico de este tipo de medicamentos.

https://doi.org/10.24875/gmm.20000241 1233 downloads

15.

Diagnóstico y tratamiento del cáncer de mama HER2+: Guía de Práctica Clínica de la Sociedad Peruana de Cancerología
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Morante, Zaida D.

; Rebaza, Lia P.

; Castañeda, Carlos A.

; Luyo, Gustavo D.

; Neciosup, Silvia P.

; Vera, Luis A.

; Aliaga, Rolig A.

; Aliaga, Karina M.

; Cotrina, José M.

; Guerra, Rolando H.

; Fuentes, Hugo A.

; Malca, Jenny

; Ballón, Karinthia

; Fuentes Rivera, Paola

; Negreiros, Tatiana

; Mendoza, Zulma K.

; Hijar, Gisely

; Aramburu, Adolfo

; Gómez, Henry L.

Anales de la Facultad de Medicina

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oct 2020, Volume 81 N. 4 Pages 458 - 465

Abstract: En Es | Text: En Es | PDF: En | PDF: Es

ABSTRACT Introduction. In Peru, breast cancer represents the most common type of cancer in women and the sixth most lethal type of cancer in the general population. Overexpression of the epidermal growth factor receptor (HER2 +) occurs in 20% to 30% of breast cancers, and is associated with more aggressive tumors, with greater recurrence and greater mortality. Objective. Prepare a set of evidence-based recommendations for the diagnosis and treatment of HER2 + breast cancer, in order to help reduce mortality, disease progression and improve quality of life. Methods. A panel of clinical specialists and methodologists was formed, who identified relevant clinical questions about the diagnosis and treatment of HER2 + breast cancer. A systematic search for CPGs was carried out in Medline (PubMed), and in developing and compiling agencies. For the formulation of recommendations, the panel of specialists discussed the evidence and elements of the context of implementation of the recommendation, following the methodology proposed by the Ministry of Health of Peru. Results. Nine clinical questions were prioritized. A total of 25 clinical recommendations were made. Conclusions. An evidence-based CPG was developed through a systematic, rigorous and transparent process developed by a multidisciplinary team.

RESUMEN Introducción. En Perú, el cáncer de mama representa el tipo de cáncer más frecuente en mujeres y el sexto tipo de cáncer más letal en la población general. La sobreexpresión del receptor del factor de crecimiento epidérmico (HER2+) ocurre en 20% a 30% de los cánceres de mama, y se asocia con tumores más agresivos, con mayor recurrencia y mayor mortalidad. Objetivo. Elaborar un conjunto de recomendaciones basadas en evidencias para el diagnóstico y tratamiento del cáncer de mama HER2+, con la finalidad de contribuir a reducir la mortalidad, progresión de la enfermedad y mejorar la calidad de vida. Métodos. Se conformó un panel de especialistas clínicos y metodólogos, quienes identificaron preguntas clínicas relevantes sobre el diagnóstico y tratamiento del cáncer de mama HER2+. Se desarrolló una búsqueda sistemática de GPC en Medline (PubMed), y en organismos elaboradores y recopiladores. Para la formulación de recomendaciones, el panel de especialistas discutió la evidencia y elementos del contexto de implementación de la recomendación, siguiendo la metodología propuesta por el Ministerio de Salud del Perú. Resultados. Se priorizó nueve preguntas clínicas. Se formuló un total de 25 recomendaciones clínicas. Conclusiones. Se elaboró una GPC basada en evidencias, a través de un proceso sistemático, riguroso y transparente desarrollado por un equipo multidisciplinario.

https://doi.org/10.15381/anales.v81i4.18839

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