Abstract Background Mutations in isocitrate dehydrogenase 1 and 2 (IDH1 and IDH2), are present in most gliomas. IDH1 mutation is an important prognostic marker in glioma. However, its regulatory mechanism in glioma remains incompletely understood. Results miR-182-5p expression was increased within IDH1-mutant glioma specimens according to TCGA, CGGA, and online dataset GSE119740, as well as collected clinical samples. (R)-2-hydroxyglutarate ((R)-2HG) treatment up-regulated the expression of miR-182-5p, enhanced glioma cell proliferation, and suppressed apoptosis; miR-182-5p inhibition partially eliminated the oncogenic effects of R-2HG upon glioma cells. By direct binding to Cyclin Dependent Kinase Inhibitor 2 C (CDKN2C) 3’UTR, miR-182-5p inhibited CDKN2C expression. Regarding cellular functions, CDKN2C knockdown promoted R-2HG-treated glioma cell viability, suppressed apoptosis, and relieved cell cycle arrest. Furthermore, CDKN2C knockdown partially attenuated the effects of miR-182-5p inhibition on cell phenotypes. Moreover, CDKN2C knockdown exerted opposite effects on cell cycle check point and apoptosis markers to those of miR-182-5p inhibition; also, CDKN2C knockdown partially attenuated the functions of miR-182-5p inhibition in cell cycle check point and apoptosis markers. The engineered CS-NPs (antagomir-182-5p) effectively encapsulated and delivered antagomir-182-5p, enhancing anti-tumor efficacy in vivo, indicating the therapeutic potential of CS-NPs(antagomir-182-5p) in targeting the miR-182-5p/CDKN2C axis against R-2HG-driven oncogenesis in mice models. Conclusions These insights highlight the potential of CS-NPs(antagomir-182-5p) to target the miR-182-5p/CDKN2C axis, offering a promising therapeutic avenue against R-2HG's oncogenic influence to glioma.

Abstract Objectives The pathological mechanisms of patients with Renal Cell Carcinoma (RCC) remain defined. This study aimed to evaluate relationships between the landscape of gene mutations and their clinical significance in RCC patients. Methods Tissue and peripheral blood samples of 42 patients with RCC were collected and performed for the Next Generation Sequencing (NGS) with Geneseeq PrimeTM 425-gene panel probes. Their landscapes of gene mutation were analyzed. We also carried out an evaluation of Tumor-Node-Metastasis (TNM) staging, RENAL nephelometry score, surgery, and targeted drug treatment of patients. Then we compared the correlations of landscape in gene mutations and the prognosis. Results The most common gene alternations, including BAP1, PBRM1, SETD2, CSF1R, NPM1, EGFR, POLE, RB1, and VHL genes, were identified in tissue and blood samples of 75% of patients. EGFR, POLE, and RB1 gene mutations frequently occurred in relapsed and metastatic patients. BAP1, CCND2, KRAS, PTPN11, ERBB2/3, JAK2, and POLE were presented in the patients with > 9 RENAL nephelometry score. Univariable analysis indicated that SETD2, BAP1, and PBRM1 genes were key factors for Disease-Free Survival (DFS). Multivariable analysis confirmed that mutated SETD1, NPM1, and CSF1R were critical factors for the Progression Free Survival (PFS) of RCC patients with target therapy. Conclusions Wild-type PBRM1 and mutated BAP1 in patients with RCC were strongly associated with the outcomes of the patient. The PFS of the patients with SETD2, NPM1, and CSF1R mutations were significantly shorter than those patients without variants. (RCC defined 4 NGS (NGS 425gene 425 probes analyzed TumorNodeMetastasis Tumor Node Metastasis TNM (TNM staging score surgery prognosis alternations BAP PBRM SETD2 SETD CSFR CSF R NPM1 NPM EGFR RB 75 CCND2 CCND KRAS PTPN11 PTPN ERBB23 ERBB ERBB2 3 ERBB2/3 JAK2 JAK DiseaseFree Disease DFS. DFS . (DFS) SETD1 (PFS therapy Wildtype Wild type patient variants 7 PTPN1 ERBB2/ (DFS

ABSTRACT The whole mitochondrial genome of Lateolabrax maculatus (Cuvier, 1828) was used to investigate the reasons for the observed patterns of genetic differentiation among 12 populations in northern and southern China. The haplotype diversity and nucleotide diversity of L. maculatus were 0.998 and 0.00169, respectively. Pairwise FST values between populations ranged from 0.001 to 0.429, correlating positively with geographic distance. Genetic structure analysis and haplotype network analysis indicated that these populations were split into two groups, in agreement with geographic segregation and environment. Tajima’s D values, Fu’s Fs tests and Bayesian skyline plot (BSP) indicated that a demographic expansion event may have occurred in the history of L. maculatus. Through selection pressure analysis, we found evidence of significant negative selection at the ATP6, ND3, Cytb, COX3, COX2 and COX1 genes. In our hypotheses, this study implied that demographic events and selection of local environmental conditions, including temperature, are responsible for population divergence. These findings are a step forward toward the understanding of the genetic basis of differentiation and adaptation, as well as conservation of L. maculatus.

Microstructural evolution, interfacial reaction and fracture during vacuum brazing of 50Mo-50Re alloys using amorphous alloy Fe-5.6Si-2.6B(wt%) were investigated. A binary intermetallic compound, σ(Mo2Re3) formed as parallel contiguous layers in the diffusion zone. In addition, Fe from the braze alloy, in combination with dissolved Mo from the substrate, formed two layers of binary compounds µ-Mo6Fe7 and λ-MoFe2, adjacent to diffusion zone. The unreacted Fe-based filler metal solidified as α-Fe solid solution. Fracture analysis showed that the mode of failure was mainly transgranular cleavage with partly intergranular and the fracture located on the interface of 50Mo-50Re alloy and the central area of brazing seam.

Resumo Introdução: Na última década, houve um uso crescente de placas biomateriais na regeneração de perfurações traumáticas da membrana timpânica. As principais vantagens das placas de biomateriais são restaurar provisoriamente a função fisiológica da orelha média, assim melhoram imediatamente os sintomas da orelha e atuam como um suporte para a migração do epitélio. No entanto, não se sabe se há efeitos clínicos adicionais na regeneração do tímpano em relação ao fragmento de material biológico. Objetivo: Avaliar a resposta de cicatrização para diferentes padrões de reparo em perfurações de membrana timpânica traumáticas humanas por meio de observação endoscópica. Método: Foram alocados 114 pacientes com perfurações de membrana timpânica traumáticas sequencialmente para dois grupos: o de cicatrização espontânea (n = 57) e o tratado com esponja de Gelfoam (n = 57). A velocidade de fechamento, o tempo de fechamento e a taxa de otorreia foram comparados entre os grupos aos três meses. Resultados: Foram analisados 107 pacientes nos dois grupos (52 no de cicatrização espontânea e 55 no tratado com esponja de Gelfoam). A velocidade global de fechamento no fim do período de seguimento de três meses foi de 90,4% no grupo de cicatrização espontânea e de 94,5% no grupo tratado com esponja de Gelfoam; a diferença não foi estatisticamente significativa (p > 0,05). No entanto, o tempo total médio de fechamento foi significativamente diferente entre os dois grupos (26,8 ± 9,1 dias no de cicatrização espontânea versus 14,7 ± 9,1 dias no tratado com esponja de Gelfoam, p < 0,01). Além disso, a velocidade de fechamento não foi significativamente diferente entre o grupo de cicatrização espontânea e o grupo tratado com esponja de Gelfoam, independentemente do tamanho da perfuração. O tempo de fechamento no grupo tratado com esponjas de Gelfoam foi significativamente menor do que no grupo de cicatrização espontânea, independentemente do tamanho da perfuração (pequenas perfurações: 7,1 ± 1,6 dias vs. 12,6 ± 3,9, perfurações de tamanho médio: 13,3 ± 2,2 dias vs. 21,8 ± 4,2 dias e grandes perfurações: 21,2 ± 4,7 dias vs. 38,4 ± 5,7 dias; p < 0,01). Conclusão: Na regeneração de PMT traumáticas, a esponja de Gelfoam não só desempenha um papel de estrutura para a migração epitelial, mas também promove edema e hiperplasia de tecido de granulação nas bordas da perfuração e acelera a cicatrização do tímpano.

Abstract Introduction: In the last decade, there has been an increasing use of biomaterial patches in the regeneration of traumatic tympanic membrane perforations. The major advantages of biomaterial patches are to provisionally restore the physiological function of the middle ear, thereby immediately improving ear symptoms, and act as a scaffold for epithelium migration. However, whether there are additional biological effects on eardrum regeneration is unclear for biological material patching in the clinic. Objective: This study evaluated the healing response for different repair patterns in human traumatic tympanic membrane perforations by endoscopic observation. Methods: In total, 114 patients with traumatic tympanic membrane perforations were allocated sequentially to two groups: the spontaneous healing group (n = 57) and Gelfoam patch-treated group (n = 57). The closure rate, closure time, and rate of otorrhea were compared between the groups at 3 months. Results: Ultimately, 107 patients were analyzed in the two groups (52 patients in the spontaneous healing group vs. 55 patients in the Gelfoam patch-treated group). The overall closure rate at the end of the 3 month follow-up period was 90.4% in the spontaneous healing group and 94.5% in the Gelfoam patch-treated group; the difference was not statistically significant (p > 0.05). However, the total average closure time was significantly different between the two groups (26.8 ± 9.1 days in the spontaneous healing group vs. 14.7 ± 9.1 days in the Gelfoam patch-treated group, p < 0.01). In addition, the closure rate was not significantly different between the spontaneous healing group and Gelfoam patch-treated group regardless of the perforation size. The closure time in the Gelfoam patch-treated group was significantly shorter than that in the spontaneous healing group regardless of the perforation size (small perforations: 7.1 ± 1.6 days vs. 12.6 ± 3.9, medium-sized perforations: 13.3 ± 2.2 days vs. 21.8 ± 4.2 days, and large perforations: 21.2 ± 4.7 days vs. 38.4 ± 5.7 days; p < 0.01). Conclusion: In the regeneration of traumatic tympanic membrane perforations, Gelfoam patching not only plays a scaffolding role for epithelial migration, it also promotes edema and hyperplasia of granulation tissue at the edges of the perforation and accelerates eardrum healing.

Recentemente, a criptografia de chave pública sem certificado (CLPKC) tem sido amplamente estudada, uma vez que poderia resolver o problema de gerenciamento de certificados na criptografia de chave pública tradicional (TPKC) e o problema chave de escrow da criptografia de chave pública baseada em identidade (ID-based PKC). Para atender aos requisitos de diferentes aplicações, têm sido propostos muitos sistemas de assinatura cega sem certificado (CLBs), sistemas que utilizam o emparelhamento bilinear para a configuração de CLPKC. No entanto, a operação de emparelhamento bilinear é muito complicada. Portanto, o desempenho desses regimes CLBs não é muito satisfatório. Para resolver o problema, propomos um esquema de CLBS eficiente sem emparelhamento bilinear. Uma análise de desempenho mostra que o esquema proposto poderia reduzir os custos de computação e armazenamento. Uma análise de segurança mostra que o esquema proposto é comprovadamente seguro contra dois tipos de adversários.

Recently, the certificateless public key cryptography (CLPKC) has been studied widely since it could solve both of the certificate management problem in traditional public key cryptography (TPKC) and the key escrow problem in the identity-based public key cryptography (ID-based PKC). To satisfy requirements of different applications, many certificateless blind signature (CLBS) schemes using bilinear pairing for the CLPKC setting have been proposed. However, the bilinear pairing operation is very complicated. Therefore, the performance of those CLBS schemes is not very satisfactory. To solve the problem, we propose an efficient CLBS scheme without bilinear pairing. Performance analysis shows that the proposed scheme could reduce costs of computation and storage. Security analysis shows the proposed scheme is provably secure against both of two types of adversaries.

PROBLEM: Quick detection and response were essential for preventing outbreaks of infectious diseases after the Sichuan earthquake. However, the existing public health communication system in Sichuan province, China, was severely damaged by the earthquake. APPROACH: The Chinese Center for Disease Control and Prevention set up a mobile phone emergency reporting system. In total, 495 light-powered mobile phones were delivered to local health-care agencies in earthquake affected areas. All phones were loaded with software designed for inputting and transmitting cases of infectious disease directly to a national database for further analysis. LOCAL SETTING: The emergency reporting system was set up in 14 counties hit hardest by the earthquake in Sichuan province, China. RELEVANT CHANGES: One week after delivering mobile phones to earthquake-affected areas, the number of health-care agencies at the township level that had filed reports returned to the normal level. The number of cases reported by using mobile phones accounted for as much as 52.9% of the total cases reported weekly from 19 May to 13 July in those areas LESSONS LEARNED: The mobile phone is a useful communication tool for infectious disease surveillance in areas hit by natural disasters. Nevertheless, plans must be made ahead of time and be included in emergency preparedness programmes.

PROBLEMA: La detección y respuesta rápidas fueron esenciales para prevenir los brotes de enfermedades infecciosas después del terremoto de Sichuan. Sin embargo, el sistema de comunicación de salud pública empleado en esa provincia china se vio gravemente afectado por el terremoto. ENFOQUE: El Centro para el Control y la Prevención de Enfermedades de China estableció un sistema de notificación de emergencia a base de teléfonos móviles. Se suministraron en total 495 teléfonos móviles alimentados por energía solar a organismos locales de atención sanitaria de las zonas afectadas por el terremoto. Todos los teléfonos disponían de software diseñado para registrar y notificar los casos de enfermedades infecciosas directamente a una base de datos nacional para su posterior análisis. CONTEXTO LOCAL: El sistema de notificación de emergencia se puso en marcha en las 14 circunscripciones de la provincia de Sichuan más castigadas por el terremoto. CAMBIOS DESTACABLES: Una semana después de suministrar los teléfonos móviles a las zonas afectadas por el seísmo, el número de organismos de atención sanitaria operativos a nivel municipal que registraban y notificaban los casos había aumentado hasta alcanzar los niveles habituales. El número de casos notificados usando los teléfonos móviles representó hasta el 52,9% del total de casos notificados semanalmente entre el 19 de mayo y el 13 de julio en esas zonas. ENSEÑANZAS EXTRAÍDAS: El teléfono móvil es un valioso instrumento de comunicación para la vigilancia de las enfermedades infecciosas en las zonas afectadas por desastres naturales. Sin embargo, es preciso planificar su uso con antelación e incluir esos planes en los programas de preparación para emergencias.

PROBLÉMATIQUE: Une détection et une riposte rapides sont essentielles pour prévenir les flambées épidémiques de maladies infectieuses après le tremblement de terre du Sichuan. Cependant, le système de communication en santé publique dont disposait la province du Sichuan a été gravement endommagé par le séisme. DÉMARCHE: Le Centre chinois pour la lutte contre les maladies et leur prévention a mis en place un système de notification d'urgence par téléphone portable. Au total, 495 téléphones portables à batterie solaire ont été livrés aux agences sanitaires locales de la zone touchée par le séisme. Un logiciel conçu pour la saisie et la transmission directe à une base de données des cas de maladie infectieuse pour analyse plus poussée a été chargé sur chaque téléphone. CONTEXTE LOCAL: Le système de notification d'urgence a été mis en place dans les 14 comtés de la province du Sichuan les plus durement touchés par le séisme. MODIFICATIONS PERTINENTES: Une semaine après la livraison des téléphones portables dans les zones affectées, le nombre d'agences sanitaires au niveau des cantons envoyant des rapports était revenu à la normale. Le nombre de cas notifiés à l'aide des téléphones portables représentait près de 52,9 % du nombre total de cas notifiés par semaine entre le 19 mai et le 13 juillet dans ces zones ENSEIGNEMENTS TIRÉS: Le téléphone portable est un outil de communication utile pour la surveillance des maladies infectieuses dans les zones frappées par des catastrophes naturelles. Néanmoins, des plans doivent être établis à l'avance et intégrés aux programmes de préparation aux situations d'urgence.