In last decades, technical-scientific investigations into cultural heritage assets have increasingly become part of analysis methodologies accessible to professionals from different areas for a broad understanding of the complex material and historical nature of objects. In this interdisciplinary word, the painting “Costureiras” by Tarsila do Amaral (1886-1973), from 1950, was studied by ultraviolet-induced visible luminescence (UVL), infrared image (IR) and macro X-ray fluorescence scanning (MA-XRF) techniques. UVL and MA-XRF images enabled the exploration of the conservation status of the artwork, the creative process of the artist, and areas featuring materials distinct from the original painting. By combining the results of IR and MA-XRF images, it was confirmed that changes occurred in the creative process during the composition of the artwork. MA-XRF images further allowed the inference of the primary pigments employed by the artist, revealing that, during canvas priming, Tarsila do Amaral utilized pigments based on lead and zinc. decades technicalscientific technical scientific objects word Costureiras “Costureiras 18861973, 18861973 1886 1973 , (1886-1973) 1950 ultravioletinduced ultraviolet induced UVL, (UVL) (IR Xray X ray MAXRF MA XRF (MA-XRF techniques artwork artist priming zinc 1886197 188 197 (1886-1973 195 (UVL 188619 18 19 (1886-197 18861 1 (1886-19 (1886-1 (1886- (1886 (188 (18 (1 (

A new alkaloid, named brazoide Ε (6), three known steroids (campesterol, β-sitosterol, stigmasterol), two glucosides (3-O-β-D-glucopyranosylsitosterol and 3-O-β-D-glucopyranosyl stigmasterol) and sucrose were isolated from the leaves and stems of Justicia wasshauseniana. Their structures were characterized using high-resolution electrospray ionization mass spectrometry (HRESIMS), nuclear magnetic resonance (1D and 2D NMR), and infrared (IR) spectroscopy. To reinforce the structural proposal, an acetylated derivative (6a) was obtained and its data compared to 6. Moreover, circular dichroism spectroscopy (CD) analysis was performed to determine the absolute stereochemistry, time-dependent density functional theory (TD-DFT) at the wB97X-D/def2-TZVP level, adopting functionals M06-2X and wB97X-D and base aug-cc-pVDZ, was employed to confirm the CD analysis. alkaloid 6, 6 , (6) campesterol, campesterol (campesterol βsitosterol, βsitosterol β sitosterol, sitosterol β-sitosterol stigmasterol, stigmasterol 3OβDglucopyranosylsitosterol OβDglucopyranosylsitosterol 3 O D glucopyranosylsitosterol 3OβDglucopyranosyl OβDglucopyranosyl glucopyranosyl wasshauseniana highresolution high resolution HRESIMS, HRESIMS (HRESIMS) 1D NMR, NMR NMR) IR (IR proposal 6a a (6a Moreover (CD stereochemistry timedependent time dependent TDDFT TD DFT (TD-DFT wB97XD/def2TZVP wB97XDdef2TZVP wBXDdefTZVP wB97X D/def2 TZVP def2 wB X def level M062X MX M06 2X M wB97XD wBXD augccpVDZ, augccpVDZ aug cc pVDZ, pVDZ aug-cc-pVDZ (6 (HRESIMS def2TZVP XD XDdef wBX Ddef2 Ddef D/def M0 ( defTZVP

Chitosan (CS) blends with collagen (CL) and Aloe vera (AV) have gained significant attention in the biomedical field due to their biocompatibility, biodegradability, and potential for wound healing applications. In this study, we investigated the preparation and characterization of CS/CL and CS/AV blends using selective precipitation and co-precipitation techniques. The synthesis processes were analyzed for yield and 4-hydroxyproline content (in CS/CL blends). The materials were characterized using bromatological analyses, viscosity measurements, Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), and scanning electron microscopy (SEM). These eco-friendly methods effectively promoted interactions between the biopolymers, resulting in homogeneous blends with tunable properties. Besides, they are simple, fast, safe, and require minimal infrastructure. This study demonstrated that selective precipitation and co-precipitation are effective methods for producing chitosan/collagen and chitosan/Aloe vera blends. These methods foster interactions and miscibility between the biopolymers, as confirmed by FTIR, XRD, viscosity, and 4-Hy content analyses. They also align with green chemistry principles by avoiding organic solvents, reducing environmental risks, and minimizing waste. The high yields achieved, especially for CS/AV blends (exceeding 96%), make these materials compatible with lyophilization procedures. The absence of segregated phases after appropriate washing underscores their suitability for pharmaceutical formulations and other applications. CS (CS CL (CL AV (AV biocompatibility biodegradability applications CSCL CSAV coprecipitation co techniques 4hydroxyproline hydroxyproline 4 . blends) analyses measurements Fouriertransform Fourier transform FTIR , (FTIR) Xray X ray XRD (XRD) SEM. SEM (SEM) ecofriendly eco friendly biopolymers properties Besides simple fast safe infrastructure chitosancollagen chitosan chitosanAloe 4Hy Hy solvents risks waste achieved exceeding 96%, 96 96% 96%) procedures (FTIR (XRD (SEM 9

RESUMO Objetivou-se com esta pesquisa determinar e avaliar o comportamento da forma e do tamanho do feijão mungo durante a secagem. O feijão mungo tem sido destaque no meio científico e agronômico devido suas propriedades nutricionais, medicinais e produtivas, e o conhecimento de suas propriedades físicas em diferentes teores de água é fundamental para otimização dos processos de plantio, colheita e pós-colheita. Os grãos foram colhidos com o teor de água de aproximadamente 27% (base úmida, bu), e posteriormente submetidos à secagem artificial com ventilação natural, até atingirem o teor de água de aproximadamente 9% (bu). Ao longo do processo de secagem a forma e o tamanho dos grãos foram avaliados mediante determinação dos eixos ortogonais, comprimento, largura e espessura em 30 grãos por repetição. De posse desses dados foram calculados a circularidade, esfericidade, volume, área superficial, área projetada, relação superfície-volume e diâmetro geométrico. A secagem reduz o tamanho dos grãos de feijão mungo, sem alterar de maneira expressiva a sua forma, que pode ser considerada circular e esférica. O comprimento e a largura dos grãos se reduzem em proporção maior que à espessura. A relação superfície/volume aumenta com a secagem, e a área projetada, área superficial e diâmetro geométrico diminuem. Objetivouse Objetivou nutricionais produtivas plantio póscolheita. póscolheita pós colheita. pós-colheita 27 base úmida bu, bu , bu) natural 9 bu. . (bu) ortogonais 3 repetição circularidade esfericidade volume projetada superfícievolume superfície esférica diminuem 2 (bu

ABSTRACT This study investigates the impact of drying on the shape and size of mung beans (Vigna radiata L.), which are well-regarded for their nutritional, medicinal, and agronomic benefits. Understanding their physical properties at varying moisture levels is crucial for improving agricultural practices from planting through post-harvest. Initially harvested at about 27% moisture content (wet basis, wb), the beans were air-dried naturally to approximately 9% moisture content (wb). The analysis involved measuring the orthogonal axes, length, width, and thickness of 30 representative beans. Parameters such as circularity, sphericity, volume, surface area, projected area, surface-to-volume ratio, and geometric diameter were calculated based on these measurements. Findings indicate that drying reduces the size of mung beans but maintains their circular and spherical shape. The length and width diminish more than the thickness. As drying progresses, the surface-to-volume ratio increases, while the projected area, surface area, and geometric diameter decrease. Vigna L., L L. , L.) wellregarded well regarded nutritional medicinal benefits postharvest. postharvest post harvest. harvest post-harvest 27 wet basis wb, wb wb) airdried air dried 9 wb. . (wb) axes 3 circularity sphericity volume area surfacetovolume measurements progresses increases decrease 2 (wb

Asphaltene aggregation mechanism is still a nebulous and controversial issue. Many authors argue that the aggregation of its components is due to π-stacking interactions, nonetheless, alkyl chains, heteroatoms, and even acidic groups are also constituents of asphaltenes and can play an important role in the chemistry of asphaltene. Excimers from pyrene are easily detected by fluorescence and were used in the current study to probe the effect of the side chain on the π-stacking capacity of pyrene. Pyrene was acylated with acyl chains varying from two to twelve carbon atoms, and the effect of the variation of the acyl chain length on excimer formation was followed by fluorescence emission spectroscopy. The results revealed that as the side chain grows from two to twelve carbon atoms the excimer/monomer ratio decreases and the excimer heat of formation drops from ΔH = -6.00 to -1.29 kcal mol-1. Further, the crystal structure of octanoyl pyrene (PC8) indicates that in the crystal structure, the aromatic moiety faces the acyl chain. This result was corroborated by density functional theory (DFT) calculations. Finally, the results were compared with two different asphaltenes and indicate that π-stacking cannot be assumed as the main driving force for asphaltene aggregation. issue πstacking π stacking interactions nonetheless heteroatoms spectroscopy excimermonomer monomer 6.00 600 6 00 -6.0 1.29 129 1 29 -1.2 mol1. mol1 mol 1. mol-1 Further PC8 PC (PC8 DFT (DFT calculations Finally 6.0 60 0 -6. 1.2 12 2 -1. mol- (PC 6. -6 -1 -

RESUMO A demanda mundial por produtos madeireiros e a escassez de espécies de madeira de boa qualidade estão pressionando as florestas nativas. Esta pesquisa teve como objetivo avaliar a eficiência do óleo de candeia (Eremanthus erythropappus) bruto para melhorar a resistência da madeira de Pinus taeda aos fungos apodrecedores Rhodonia (≈Postia) placenta, Gloeophyllum trabeum, Neolentinus lepideus (podridão parda) e Pycnoporus sanguineus (podridão branca) em ensaios de apodrecimento acelerado em laboratório. As amostras tinham dimensões nominais de 1,90 × 1,90 × 1,90 cm. O óleo foi aplicado nas superfícies da madeira com pincel, visando obter retenções nominais de 15; 30; 45 e 60 kg de óleo por m3 de madeira. As amostras após o tratamento atingiram retenções médias de 16,25; 28,75; 41,50; e 53,75 kg m-3, e foram submetidas ao teste solo-bloco (apodrecimento acelerado) durante 12 semanas. O aumento da retenção de óleo provocou redução na perda de massa da madeira de Pinus taeda submetida aos fungos testados. Observou-se que a retenção de 16,25 kg m-3 foi capaz de inibir a degradação do fungo Rhodonia (≈Postia) e prevenir o ataque de outros fungos xilófagos. Os maiores danos foram observados para Rhodonia (≈Postia) e os menores danos para Pycnoporus sanguineus. O óleo conferiu maior resistência à madeira de Pinus taeda aos fungos testados, indicando ser útil para o tratamento de madeiras que ficarão em contato com animais domésticos e seres humanos, uma vez que não é toxico a tais organismos. nativas Eremanthus erythropappus ≈Postia Postia (≈Postia placenta trabeum podridão parda branca laboratório 190 1 90 1,9 cm pincel 15 30 4 6 m 1625 16 25 28,75 2875 28 75 41,50 4150 41 50 5375 53 53,7 m3, 3, 3 solobloco solo bloco semanas testados Observouse Observou se 16,2 m- xilófagos humanos organismos 19 9 1, 162 2 28,7 287 7 41,5 415 5 537 53, 16, 28, 41,

ABSTRACT World demand for wood products and the shortage of good quality lumber species are exerting pressure on native forests. This study aimed to evaluate the efficiency of Eremanthus erythropappus crude oil in improving the resistance of Pinus taeda wood to Rhodonia (≈Postia) placenta, Gloeophyllum trabeum, Neolentinus lepideus (brown rot) and Pycnoporus sanguineus (white rot) in accelerated laboratory decay tests. Test samples measured 1.90 × 1.90 × 1.90 cm. Oil was applied to wood surfaces using a brush to obtain nominal retentions of 15, 30, 45, and 60 kg of oil per m3 of wood. After treatment, the samples reached average retentions of 16.25, 28.75, 41.50, and 53.75 kg m-3, respectively, and were submitted to a soil block decay test (accelerated decay test) for 12 weeks. Increased oil retention caused a decline in the mass loss of Pinus taeda wood exposed to the fungi tested. A retention of 16.25 kg m-3 inhibited Rhodonia placenta degradation and prevented other xylophagous fungi attacks. The most severe decay occurred with Rhodonia placenta and the least with Pycnoporus sanguineus. Oil improved Pinus taeda resistance to the decay fungi, indicating that it is useful for treating wood in contact with domestic animals and humans, since it is not toxic to them. forests ≈Postia Postia (≈Postia trabeum brown rot white tests 190 1 90 1.9 cm 15 30 45 6 m treatment 1625 16 25 2875 28 75 28.75 4150 41 50 41.50 5375 53 53.7 m3, 3, 3 respectively weeks tested 16.2 m- attacks humans them 19 9 1. 4 162 2 287 7 28.7 415 5 41.5 537 53. 16. 28. 41.

Abstract Framework: The Covid-19 pandemic has brought several challenges at an economic, labor, social, and organizational level, and organizations had to adopt strategies to ensure their functioning, as well as to ensure and promote the health and well-being of workers. Research Question/Goals: This study aimed to identify and describe (1) the impact of Covid-19 on the world of work and (2) the strategies that organizations have adopted to minimize this impact. Method: A descriptive qualitative cross-sectional study was carried out, where data were collected from 18 participants (10 Human Resources Managers, five specialists from relevant fields regarding professional experiences with Covid-19, and three representatives of public entities and Portuguese civil society from the fields of work and gender) through semi-structured interviews. The data were analyzed using thematic categorical content analysis with the support of NVivo®. Results: The pandemic impacted (RQ1) on work, economic and labor, work-life balance, and health and well-being. The measures (RQ2) most frequently implemented by the organizations were in terms of health and safety at work, adaptation to teleworking, work-life balance, and communication. Final considerations: The data points to increasingly remote and flexible work transformed by new technologies. In the (re)design of the work, it is essential to consider the characteristics and needs of each worker.

Resumo Introdução/Enquadramento: A pandemia Covid-19 trouxe diversos desafios a nível económico e laboral, social e organizacional, tendo as organizações que adotar estratégias para garantir o seu funcionamento, mas também garantir e promover a saúde e bem-estar dos trabalhadores. Questão de Investigação/Objetivos: Este estudo teve como objetivo identificar e descrever: (1) o impacto da Covid-19 no mundo do trabalho, e (2) as estratégias que as organizações adotaram para minimizar esse impacto. Método: Foi realizado um estudo transversal qualitativo descritivo, onde foram recolhidos dados de 18 participantes (10 Gestores de Recursos Humanos, cinco especialistas de áreas relevantes sobre as vivências profissionais com a Covid-19 e três representantes de entidades públicas e da sociedade civil portuguesa nas áreas do trabalho e do género) através de entrevistas semiestruturadas. Os dados foram analisados à luz da análise de conteúdo categorial temática, com o apoio do NVivo®. Resultados: A pandemia teve impacto (QI1) a nível do trabalho, económico e laboral, da conciliação, e da saúde e bem-estar. As medidas (QI2) mais frequentemente implementadas pelas organizações foram ao nível da higiene e segurança no trabalho, adaptação ao teletrabalho, conciliação e comunicação. Considerações finais: Os dados apontam para um mundo do trabalho cada vez mais remoto e flexível, transformado pelo uso das novas tecnologias. No (re)desenho do trabalho é essencial ter em conta as características e necessidades de cada trabalhador.

Resumo Fundamento: A Cardiomiopatia Chagásica Crônica (CCC) é causada por um processo inflamatório induzido pelo Trypanosoma cruzi, que leva à miocardite com fibrose reativa e reparativa. A CCC progride com alterações de perfusão miocárdica e eventos histopatológicos que afetam a Aptidão Cardiorrespiratória (ACR). Objetivos: Avaliamos os efeitos do Treinamento Físico Aeróbico (TFA) na perfusão miocárdica e nos comprometimentos morfológicos e funcionais relacionados à inflamação e fibrose em hamsters sírios com CCC. Como objetivo secundário, analisamos as áreas de secção transversa do músculo esquelético. Métodos: Hamsters com CCC e seus respectivos controles foram divididos em quatro grupos: CCC sedentário, CCC-TFA, controle sedentário e controle TFA. Sete meses após a infecção, os animais foram submetidos à ecocardiografia, à cintilografia de perfusão miocárdica e ao teste de esforço cardiopulmonar. TFA de intensidade moderada foi realizado durante cinquenta minutos, cinco vezes por semana, por oito semanas. Posteriormente, os animais foram reavaliados. A análise histopatológica foi realizada após os procedimentos acima mencionados. O nível de significância foi estabelecido em 5% em todas as análises (p<0,05). Resultados: Animais com CCC sedentários apresentaram piores Defeitos de Perfusão Miocárdica (DPM) ao longo do tempo, Fração de Ejeção do Ventrículo Esquerdo (FEVE) reduzida, e apresentaram mais inflamação e fibrose quando comparados aos demais grupos (análise ANOVA mista). Por outro lado, o TFA foi capaz de mitigar a progressão do DPM, atenuar a inflamação e a fibrose e melhorar a eficiência da ACR em animais CCC-TFA. Conclusão: Nosso estudo demonstrou que o TFA melhorou a disfunção cardíaca, DPM e reduziu a inflamação e a fibrose em modelos de hamster com CCC. Além disso, os animais CCC-SED apresentaram atrofia do músculo esquelético, enquanto os animais CCC-TFA apresentaram a AST do músculo esquelético preservada. Compreender os efeitos da TFA nas dimensões fisiopatológicas da CCC é crucial para futuras pesquisas e intervenções terapêuticas. Fundamento (CCC cruzi reparativa ACR. . (ACR) Objetivos (TFA secundário Métodos CCCTFA, CCCTFA TFA, infecção ecocardiografia cardiopulmonar minutos semana semanas Posteriormente reavaliados mencionados 5 p<0,05. p005 p p<0,05 0 05 (p<0,05) Resultados (DPM tempo FEVE (FEVE reduzida mista. mista mista) lado CCCTFA. Conclusão cardíaca disso CCCSED SED preservada terapêuticas (ACR p00 p<0,0 (p<0,05 p0 p<0, (p<0,0 p<0 (p<0, p< (p<0 (p< (p

Abstract Background: Chronic Chagas cardiomyopathy (CCC) is caused by an inflammatory process induced by Trypanosoma cruzi, which leads to myocarditis with reactive and reparative fibrosis. CCC progresses with myocardial perfusion abnormalities and histopathological events that affect cardiorespiratory fitness (CRF). Objectives: We evaluated the effects of aerobic physical training (APT) on myocardial perfusion and on morphological and functional impairments related with inflammation and fibrosis in Syrian hamsters with CCC. As a secondary objective, we analyzed the cross-sectional areas of the skeletal muscle. Methods: Hamsters with CCC and their respective controls were divided into four groups: CCC sedentary, CCC-APT, sedentary control and APT control. Seven months after infection, the animals underwent echocardiography, myocardial perfusion scintigraphy and cardiopulmonary exercise testing. Moderate-intensity APT was performed for fifty minutes, five times a week, for eight weeks. Subsequently, the animals were reassessed. Histopathological analysis was conducted after the above-mentioned procedures. The level of significance was set at 5% in all analyses (p<0.05). Results: CCC sedentary animals presented worse myocardial perfusion defects (MPD) over time, reduced left ventricle ejection fraction (LVEF) and showed more inflammation and fibrosis when compared to other groups (mixed ANOVA analysis). Conversely, APT was able to mitigate the progression of MPD, ameliorate inflammation and fibrosis and improve CRF efficiency in CCC-APT animals. Conclusions: Our study demonstrated that APT ameliorated cardiac dysfunction, MPD, and reduced inflammation and fibrosis in CCC hamster models. Additionally, CCC-SED animals presented skeletal muscle atrophy while CCC-APT animals showed preserved skeletal muscle CSA. Understanding APT's effects on CCC's pathophysiological dimensions is crucial for future research and therapeutic interventions. Background (CCC cruzi CRF. . (CRF) Objectives (APT objective crosssectional cross sectional Methods CCCAPT, CCCAPT APT, infection echocardiography testing Moderateintensity Moderate intensity minutes week weeks Subsequently reassessed abovementioned above mentioned procedures 5 p<0.05. p005 p p<0.05 0 05 (p<0.05) Results MPD (MPD time LVEF (LVEF mixed analysis. analysis) Conversely Conclusions dysfunction models Additionally CCCSED SED CSA APTs s CCCs interventions (CRF p00 p<0.0 (p<0.05 p0 p<0. (p<0.0 p<0 (p<0. p< (p<0 (p< (p

RESUMO A entalpia tem sido proposta atualmente como um índice adequado para a avaliação do ambiente interno de galpões de frangos de corte, pois depende basicamente da temperatura e da umidade relativa do ar. Dessa forma, objetivou-se analisar a entalpia como índice de conforto térmico na produção de aves para o município de Londrina, Paraná, e modelar a entalpia aos índices de conforto térmico que proporcionam eficiência na produção. Para o estudo, utilizaram-se dados de 1961 a 2021 do Instituto Nacional de Meteorologia (INMET), de Brasília, Distrito Federal. Os dados meteorológicos desse período foram analisados para conhecer suas variações e as relações entre eles, através de um estudo descritivo e de correlação entre diferentes parâmetros, para comparar as condições de conforto térmico e os limites críticos estabelecidos para as aves. Com base nos resultados desta pesquisa, considerando os índices de entalpia obtidos através de médias históricas, pode-se concluir que a utilização de aquecimento para as aves na primeira e segunda semanas de alojamento, no período de inverno, considerando o local estudado, é imprescindível, devido às baixas temperaturas registadas. Para aves alojadas na terceira, quarta, quinta e sexta semana de idade, considerando os índices de entalpia, pode-se concluir que é necessária a utilização de sistemas de resfriamento para as aves. corte ar forma objetivouse objetivou se Londrina Paraná utilizaramse utilizaram 196 202 INMET, INMET , (INMET) Brasília Federal eles parâmetros pesquisa históricas podese pode alojamento inverno estudado imprescindível registadas terceira quarta idade 19 20 (INMET 1 2

ABSTRACT Enthalpy is currently suggested as a suitable index for assessing the internal environment of poultry production, primarily dependents on air temperature and relative air humidity. This study aimed to analyze enthalpy in bird production in Londrina, located in the northern region of Paraná, Brazil, and to model the comfort enthalpy required for enhanced production efficiency. Data from 1961 to 2021 from the Instituto Nacional de Meteorologia (INMET), Brasília, Federal District, Brazil, were utilized to compare the thermal comfort conditions and critical limits set for the birds. The meteorological data from this period were scrutinized to understand their variations and interrelationship through a descriptive study and correlation analysis of different parameters. The research findings, based on the enthalpy indices derive historical averages, suggest that heating is crucial for birds during the first and second weeks of housing, during the winter, given the location’s low temperatures. For birds housed during the third to sixth weeks of age, the enthalpy indices indicated the necessity of cooling systems. humidity Londrina Paraná Brazil efficiency 196 202 INMET, INMET , (INMET) Brasília District parameters findings averages housing winter locations location s temperatures age systems 19 20 (INMET 1 2

Resumo A leishmaniose visceral (LV) é uma doença de natureza infecciosa, predominante em países de zonas tropicais. A predição de ocorrência de doenças infecciosas através da modelagem epidemiológica tem se revelado uma importante ferramenta no entendimento de sua dinâmica de ocorrência. O objetivo deste estudo foi desenvolver um modelo de previsão da incidência da LV no Maranhão usando o modelo de Média Móvel Integrada Autocorrelacionada Sazonal (SARIMA). Foram coletados os dados mensais de casos de LV através do Sistema de Informação de Agravos de Notificação (SINAN) correspondentes ao período de 2001 a 2018. O método de Box-Jenkins foi aplicado para ajustar um modelo de predição SARIMA para incidência geral e por sexo (masculino e feminino) de LV para o período de janeiro de 2019 a dezembro de 2023. Durante o período de 216 meses dessa série temporal, foram registrados 10.431 casos de LV no Maranhão, com uma média de 579 casos por ano. Em relação à faixa etária, houve maior registro no público pediátrico (0 a 14 anos). Houve predominância do sexo masculino, com 6437 casos (61,71%). Os valores do teste de Box-Pierce para incidência geral, sexo masculino e feminino reforçados pelos resultados do teste Ljung-Box sugerem que as autocorrelações de resíduos apresentam um comportamento de ruído branco. Para incidência mensal geral e por sexo masculino e feminino, os modelos SARIMA (2,0,0) (2,0,0), (0,1,1) (0,1,1) e (0,1,1) (2, 0, 0) foram os que mais se ajustaram aos dados, respectivamente. O modelo SARIMA se mostrou uma ferramenta adequada de previsão e análise da tendência de incidência da LV no Maranhão. A determinação da variação temporal e sua predição são determinantes no norteamento de medidas de intervenção em saúde.

Abstract Visceral leishmaniasis (VL) is an infectious disease predominant in countries located in the tropics. The prediction of occurrence of infectious diseases through epidemiologic modeling has revealed to be an important tool in the understanding of its occurrence dynamic. The objective of this study was to develop a forecasting model for the incidence of VL in Maranhão using the Seasonal Autoregressive Integrated Moving Average model (SARIMA). We collected monthly data regarding VL cases from the National Disease Notification System (SINAN) corresponding to the period between 2001 and 2018. The Box-Jenkins method was applied in order to adjust a SARIMA prediction model for VL general incidence and by sex (male or female) for the period between January 2019 and December 2013. For 216 months of this time series, 10,431 cases of VL were notified in Maranhão, with an average of 579 cases per year. With regard to age range, there was a higher incidence among the pediatric public (0 to 14 years of age). There was a predominance in male cases, 6437 (61.71%). The Box-Pierce test figures for overall, male and female genders supported by the results of the Ljung-Box test suggest that the autocorrelations of residual values act as white noise. Regarding monthly occurrences in general and by gender, the SARIMA models (2,0,0) (2,0,0), (0,1,1) (0,1,1) and (0,1,1) (2, 0, 0) were the ones that mostly adjusted to the data respectively. The model SARIMA has proven to be an adequate tool for predicting and analyzing the trends in VL incidence in Maranhão. The time variation determination and its prediction are decisive in providing guidance in health measure intervention.

Resumo Em condições de estresse salino, o crescimento das plantas é reduzido, em virtude, do desequilíbrio osmótico, nutricional e oxidativo. Contudo, o ácido salicílico atua na mitigação desse estresse abiótico por promover incremento no crescimento, fotossíntese, metabolismo do nitrogênio, síntese de osmorreguladores e enzimas antioxidantes. Nesse contexto, objetivou-se avaliar o efeito de doses de ácido salicílico sobre o crescimento e alterações fisiológicas de mudas de berinjela sob estresse salino. O experimento foi conduzido em casa de vegetação, onde os tratamentos foram distribuídos em blocos ao acaso utilizando uma matriz composta central Box com cinco níveis de condutividade elétrica da água de irrigação (CEa) (0,50; 1,08; 2,50; 3,92 e 4,50 dS m-1), associada a cinco doses de ácido salicílico (AS) (0,00; 0,22; 0,75; 1,28 e 1,50 mM), com quatro repetições e cada parcela composta por três plantas. Aos 40 dias após a semeadura, foram determinados a altura da planta, diâmetro do caule, número de folhas, área foliar, vazamento de eletrólito, teor relativo de água e massa seca total. A CEa e a aplicação de AS influenciaram no crescimento e nas alterações fisiológicas das mudas de berinjela. O aumento da CEa reduziu o crescimento na ausência de AS. O dano de membrana com o uso de AS manteve-se estável até 3,9 dS m-1 de CEa. O conteúdo relativo de água independentemente da CEa aumentou com 1 mM de SA. O uso de AS na concentração de 1 mM mitigou o efeito deletério da salinidade no crescimento das mudas até 2,50 dS m-1 de CEa.

Abstract Under salt stress conditions, plant growth is reduced due to osmotic, nutritional and oxidative imbalance. However, salicylic acid acts in the mitigation of this abiotic stress by promoting an increase in growth, photosynthesis, nitrogen metabolism, synthesis of osmoregulators and antioxidant enzymes. In this context, the objective was to evaluate the effect of salicylic acid doses on the growth and physiological changes of eggplant seedlings under salt stress. The experiment was conducted in a greenhouse, where the treatments were distributed in randomized blocks using a central composite matrix Box with five levels of electrical conductivity of irrigation water (CEw) (0.50; 1.08; 2.50; 3.92 and 4.50 dS m-1), associated with five doses of salicylic acid (SA) (0.00; 0.22; 0.75; 1.28 and 1.50 mM), with four repetitions and each plot composed of three plants. At 40 days after sowing, plant height, stem diameter, number of leaves, leaf area, electrolyte leakage, relative water content, and total dry mass were determined. ECw and SA application influenced the growth and physiological changes of eggplant seedlings. Increasing the ECw reduced growth in the absence of SA. Membrane damage with the use of SA remained stable up to 3.9 dS m-1 of ECw. The relative water content independent of the CEw increased with 1.0 mM of SA. The use of SA at the concentration of 1.0 mM mitigated the deleterious effect of salinity on seedling growth up to 2.50 dS m-1 of ECw.

The compound 2-hydrazinyl-4-(4-methoxyphenyl)thiazole (PD76) is a novel thiazolyl hydrazine derivative with proven antifungal activity against different fungal species, mainly Candida and Cryptococcus. Considering the advantages of oral route for clinical therapy, the aim of this work was to evaluate the potential intestinal permeability of this new antifungal drug. For the quantitation of PD76, a high-performance liquid chromatography method was developed and fully validated. The cytotoxicity of the compound in Caco-2 cells was analyzed and intestinal permeability of PD76 was assessed by means of the comparison of in vitro assay in Caco-2 cells and in silico platforms ADMETlab and admetSAR. Cell viability above 70% was obtained at all PD76 studied concentrations. Using Caco-2 cell model, the compound showed apparent permeability coefficients (Papp) of 5.25 × 10-6 and 23.28 × 10-6 cm s-1 in apical-basolateral and basolateral-apical directions, respectively. Experiments performed using verapamil as P-gp inhibitor demonstrated that PD76 is slightly susceptible to active efflux. Both in silico platforms inferred that PD76 presents permeability in Caco-2 cells, with Log P values of 2.82 (ADMETlab) and 2.10 (admetSAR). The results obtained in permeability studies showed that PD76 presents moderate intestinal permeability and a promising profile for clinical application. 2hydrazinyl44methoxyphenylthiazole hydrazinylmethoxyphenylthiazole 2 hydrazinyl 4 methoxyphenyl thiazole PD (PD76 species Cryptococcus therapy drug highperformance high performance validated Caco2 Caco Caco- PD7 admetSAR 70 concentrations model Papp (Papp 525 5 25 5.2 106 10 6 10- 2328 23 28 23.2 s1 s 1 s- apicalbasolateral apical basolateral basolateralapical directions respectively Pgp gp efflux 282 82 2.8 (ADMETlab 210 2.1 . (admetSAR) application methoxyphenylthiazole (PD7 7 52 5. 232 23. 8 2. 21 (admetSAR (PD

RESUMO Ligas Al-Cu-Li têm apresentado vantagens em relação às ligas Al-Cu-Mg convencionais usadas na indústria aeroespacial devido as suas propriedades, como alta resistência à fadiga e baixa densidade. As propriedades mecânicas destas ligas dependem de sua microestrutura. Neste trabalho, a atividade eletroquímica associada a microestrutura e comportamento à corrosão da liga Al-Cu-Li 2198-T8 foi investigada por testes eletroquímicos e ensaios de imersão em soluções contendo íons cloreto (Cl–). Os resultados obtidos mostraram que o comportamento eletroquímico e a susceptibilidade a corrosão desta liga são fortemente influenciados pela fase T1(Al2CuLi), a qual é a principal responsável pelo endurecimento da mesma. Foi observado ainda que esta fase é eletroquimicamente mais ativa do que a matriz de alumínio e, que quando exposta ao meio corrosivo, resulta em corrosão localizada severa (CLS), devido ao ataque cristalográfico. Nestas regiões foi observada intensa acidificação, principalmente dentro dos pites, resultando na evolução de gás hidrogênio, o que foi confirmado pelos ensaios de visualização em gel. Os resultados obtidos por técnicas eletroquímicas globais e técnica de varredura do eletrodo vibratório (SVET) confirmaram as observações dos ensaios de imersão e de visualização em gel. AlCuLi Al Cu Li AlCuMg Mg densidade trabalho 2198T8 T 2198 T8 2198-T Cl–. Cl Cl– . (Cl–) T1Al2CuLi, T1Al2CuLi TAlCuLi T1 Al2CuLi , CuLi T1(Al2CuLi) mesma corrosivo CLS, CLS (CLS) cristalográfico acidificação pites hidrogênio gel SVET (SVET 2198T 219 (Cl– T1(Al2CuLi (CLS 21 (Cl 2

ABSTRACT Al-Cu-Li alloys present interesting advantages over conventional Al-Cu-Mg alloys used in the aerospace industry, due to their excellent properties, such as high fatigue resistance and low density. The mechanical properties of these alloys depend on their microstructure. In this work, the electrochemical activity associated with the microstructure and corrosion behavior of the AA2198-T8, Al-Cu-Li alloy, was investigated through electrochemical and immersion tests, in chloride ions (Cl–) containing solutions. Results obtained showed that the electrochemical behavior and susceptibility to corrosion of this alloy are strongly influenced by the T1(Al2CuLi) phase density which is mainly responsible for its hardening. It was also observed that this phase is electrochemically more active than the aluminum matrix and when exposed to corrosive environments, it results in severe localized corrosion (SLC), due to crystallographic attack. In these regions, intense acidification was observed, within the pits, resulting in hydrogen gas evolution, which was confirmed by gel visualization tests. The results obtained through global electrochemical techniques and scanning vibrating electrode technique (SVET) confirmed the observations from the immersion and gel visualization tests, where increased values of anodic current densities were related to the SLC regions. AlCuLi Al Cu Li AlCuMg Mg industry work AA2198T8, AA2198T8 AAT AA2198 T8, T8 AA T AA2198-T8 tests Cl– Cl (Cl– solutions T1Al2CuLi TAlCuLi T1 Al2CuLi CuLi T1(Al2CuLi hardening environments SLC, , (SLC) attack regions pits evolution SVET (SVET AA2198T AA219 AA2198-T (Cl (SLC AA21 AA2

ABSTRACT The varied clinical presentations of SARS-CoV-2 infection have raised concerns about long-term consequences, especially “long-COVID” or “post-COVID-19 syndrome.” In this context, the cardiorespiratory optimal point (COP) within the Cardiopulmonary Exercise Test (CPET) emerges as a crucial metric for evaluating functional capacities and detecting cardiovascular and pulmonary anomalies post-COVID-19. This study aimed to assess COP values among post-COVID-19 patients and categorized them based on the initial severity of their disease. In this cross-sectional study conducted in the Northeast Brazil, 80 patients (26 females and 54 males) previously infected with SARS-CoV-2 underwent CPET. We clinically stratified patients into mild, moderate, or severe COVID-19 categories and assessed COP values and other cardiorespiratory metrics. We found differences in the predicted COP between patients with mild and severe COVID-19 (p=0.042). Additionally, patients with moderate and severe COVID-19 record had an average COP value exceeding 22. Other parameters, including respiratory exchange ratio, heart rate, and oxygen uptake efficiency slope, did not differ across the groups. Patients with a history of severe COVID-19 showed altered COP values, suggesting potential discrepancies in cardiovascular and respiratory system integration. The outcomes emphasize the importance of continuous monitoring and assessment of the cardiorespiratory domain for post-COVID-19 patients. Further research is needed to understand the relationship between elevated COP in post-severe COVID-19 and its long-term prognostic implications. SARSCoV2 SARSCoV SARS CoV 2 SARS-CoV- longterm long term consequences longCOVID COVID “long-COVID postCOVID19 postCOVID post 19 “post-COVID-1 syndrome. syndrome context (COP CPET (CPET postCOVID19. 19. post-COVID-1 disease crosssectional cross sectional Brazil 8 26 (2 5 males COVID19 COVID-1 metrics p=0.042. p0042 p p=0.042 . 0 042 (p=0.042) Additionally 22 parameters ratio rate slope groups integration postsevere implications SARS-CoV postCOVID1 1 “post-COVID- post-COVID- ( COVID1 COVID- p004 p=0.04 04 (p=0.042 “post-COVID post-COVID p00 p=0.0 (p=0.04 p0 p=0. (p=0.0 p=0 (p=0. p= (p=0 (p= (p

ABSTRACT Post-acute COVID-19 syndrome, or long COVID, presents with persistent symptoms, including cough, dyspnea, and fatigue, extending beyond one month after SARS-CoV-2 infection. Cardiac complications such as chest pain and arrhythmias have raised concerns, with chronotropic incompetence (CI), an inadequate heart rate increase during exercise, emerging as a significant condition contributing to diminished exercise tolerance and quality of life. This study estimated the prevalence of CI and explored its association with aerobic capacity and physical activity levels in long COVID patients. A cross-sectional study was conducted at a private hospital in Sergipe, Brazil, involving 93 patients over 18 years old with persistent post-COVID-19 symptoms after confirmed SARS-CoV-2 infections. Exclusion criteria included beta-blocker use, inadequate respiratory exchange ratio, and inability to complete cardiopulmonary exercise testing (CPET). Clinical histories, CPET results, and chronotropic index calculation were used to identify CI, with logistic regression analyzing associated factors. Of the participants (mean age 45 years; average duration since COVID-19 diagnosis 120 days), 20.4% were diagnosed with CI. Logistic regression identified a strong association between CI and sedentary behavior (OR 11.80; 95% CI 2.54 to 54.78; p=0.001). Patients with CI showed lower predicted peak heart rates and maximal oxygen uptake. The prevalence of CI among long COVID patients in this study was approximately 20%, associated with decreased aerobic capacity and increased sedentary behavior. These findings highlight the need for timely diagnosis and therapeutic interventions, including cardiopulmonary rehabilitation, to enhance the quality of life in post-COVID patients with CI. The study’s cross-sectional design and its specific context have limited causality inference and generalizability, underscoring the importance of further research in diverse settings. Postacute Post acute COVID19 19 COVID-1 syndrome cough dyspnea fatigue SARSCoV2 SARSCoV SARS CoV 2 SARS-CoV- infection concerns , (CI) crosssectional cross sectional Sergipe Brazil 9 1 postCOVID19 postCOVID post post-COVID-1 infections betablocker beta blocker use ratio CPET. . (CPET) histories results factors mean 4 12 days, days days) 204 20 20.4 OR 11.80 1180 11 80 95 254 54 2.5 54.78 5478 78 p=0.001. p0001 p p=0.001 0 001 p=0.001) uptake 20% interventions rehabilitation studys s generalizability settings COVID1 COVID- SARS-CoV (CI postCOVID1 post-COVID- (CPET 20. 11.8 118 8 25 5 2. 54.7 547 7 p000 p=0.00 00 11. 54. p00 p=0.0 p0 p=0. p=0 p=