The rapid advance of nanotechnology has led to the proliferation of a wide range of water-soluble nanomaterials and therefore, a profound impact on the environment, underscoring the need to devise effective methods for isolating and confining these nanomaterials. This study presents the development of a highly effective system capable of removing and storing gold nanoparticles in solution using modified resins with different linking groups, such as tris(2-aminoethyl) amine, 3-mercaptopropionic acid, 11-mercaptoundecanoic acid, and L-cysteine. The catalytic properties of the resins functionalized with the thiol group containing gold nanoparticles were evaluated, using the reduction of the nitro compounds (3,5-dinitrosalicylic acid, 4-nitroguaiacol and 4-nitroaniline) as model reaction. Solid-phase organic synthesis was employed to prepare the modified resins and their interaction with gold nanoparticles was characterized using infrared spectroscopy, thermogravimetric analysis, field emission scanning electron microscopy-energy dispersive spectroscopy, and X-ray photoelectron spectroscopy. The removal capacity of the resins was evaluated using ultraviolet-visible optical emission spectroscopy and inductively coupled plasma techniques. Ultraviolet-visible technique was suitable to assess the catalytic properties of the functionalized resins containing gold nanoparticles. It was determined that the functionalized resins showed remarkable capacity for complex gold nanoparticles, removing them from the solution. The most significant removal was with 11-mercaptoundecanoic acid. watersoluble water soluble therefore environment groups tris2aminoethyl trisaminoethyl tris 2 aminoethyl tris(2-aminoethyl amine 3mercaptopropionic mercaptopropionic 3 acid 11mercaptoundecanoic mercaptoundecanoic 11 Lcysteine. Lcysteine L cysteine. cysteine L-cysteine 3,5dinitrosalicylic 35dinitrosalicylic dinitrosalicylic 3,5 5 4nitroguaiacol nitroguaiacol 4 4nitroaniline nitroaniline 4-nitroaniline reaction Solidphase Solid phase analysis microscopyenergy microscopy energy Xray X ray ultravioletvisible ultraviolet visible techniques Ultravioletvisible Ultraviolet 1 5dinitrosalicylic 35 3,

Lisdexamfetamine is a prodrug that is converted to d-amphetamine in the body, mainly used to treat attention deficit hyperactivity disorder in children and adults. Its mechanism involves the enhancement of neurotransmitters such as norepinephrine and dopamine, which are critical for attention and behavioral regulation. With the upcoming patent expiration of Vyvanse®, bioanalytical methods for the quantification of lisdexamfetamine in plasma are needed for use in pharmacokinetic studies. In this work, we developed and validated a sensitive bioanalytical method using liquid chromatography-tandem mass spectrometry (LC-MS/MS) for lisdexamfetamine quantification in human plasma. The method developed was performed for 4 min per sample and using the concentration range of 0.3-100 ng mL-1. Intra-batch, inter-batch and instrument reproducibility, the precision was lower than 10%. The results demonstrated that the method is rapid, sensitive, robust, and suitable for pharmacokinetic studies. damphetamine d amphetamine body adults dopamine regulation Vyvanse Vyvanse® studies work chromatographytandem chromatography tandem LCMS/MS LCMSMS LC MS/MS MS (LC-MS/MS 0.3100 03100 0.3 100 0 3 0.3-10 mL1. mL1 mL 1. 1 mL-1 Intrabatch, Intrabatch Intra batch, batch Intra-batch interbatch inter reproducibility 10 10% rapid robust LCMS MSMS 3100 0.310 0310 03 0. 0.3-1 mL- 310 0.31 031 0.3- 31

Abstract Objective: To describe the changes in alcohol and drug use by Brazilian adolescents during the COVID-19 pandemic (April-August 2021), and to analyze the relationship between alcohol use changes and psychiatric symptomatology. Methods: A secondary analysis with a longitudinal approach was performed with data from a cluster-randomized controlled trial, conductedin73publicmiddleschoolsinthreeBrazilian cities, to evaluate the effectiveness of a drug use prevention program. The sample included 535 students (61% girls; Mage = 15.2 years). Data were collected pre-intervention (February-March 2019), after 9 months (November-December 2019), and after 26 months (April-August 2021), when the students were in their first year of high school. The authors analyzed drug use prevalence (alcohol, binge drinking, tobacco, inhalants, marijuana, cocaine, and crack) in a lifetime, past year, and past month periods, and the association between alcohol use change subsamples with psychiatric symptoms. Logistic regressions were adjusted by sex, age, socioeconomic status, city, and group (control and intervention). Results: The present findings suggest that the COVID-19 pandemic led to a decrease in past-year substance use and in past-month substance use frequency, despite the gradually increased (but decelerating) prevalence of lifetime use. However, some adolescents initiated, maintained, or increased the frequency of their alcohol use. Mainly, they were more likely to present behavioral problems, as well as symptoms of inattentive hyperactivity, and peer and emotional problems. Conclusions: Despite the extensive decline in substance use during the pandemic period, these results suggest an association between previous mental health conditions and behavioral risk factors, leading to increased alcohol consumption and behavioral disorders manifestations. Objective COVID19 COVID 19 COVID-1 AprilAugust April August 2021, 2021 , 2021) symptomatology Methods clusterrandomized cluster randomized trial conductedinpublicmiddleschoolsinthreeBrazilian conductedin publicmiddleschoolsinthreeBrazilian cities program 53 61% 61 (61 girls 152 15 2 15. years. years . years) preintervention pre intervention FebruaryMarch February March 2019, 2019 2019) NovemberDecember November December school alcohol, (alcohol drinking tobacco inhalants marijuana cocaine crack periods sex age status city control intervention. intervention) Results pastyear pastmonth but decelerating However initiated maintained Mainly problems hyperactivity Conclusions period factors manifestations COVID1 1 COVID- 202 5 6 (6 201 20 (

ABSTRACT We report an 82-year-old male from Iquitos, Amazon region of Peru, with a three-month history of mild-to-moderate painful skin lesions on the right lower limb. The clinical examination revealed four tumors of the right shin, exhibiting exophytic growth, verrucose appearance, and ulceration. Two subcutaneous nodules were noted. Enlarged inguinal lymphadenopathy was observed. The histopathological examination revealed a diagnosis of melanoma. This case emphasizes the need for comprehensive and multidisciplinary surveillance in regions with limited melanoma data leading to a subdiagnosis of this malignancy. Also, it highlights the importance of primary prevention such as sun protection in tropical areas to prevent cutaneous melanoma, advocating for increasing awareness and preventive measures.

RESUMEN Reportamos el caso de un hombre de 82 años residente de Iquitos, región amazónica del Perú, con una historia de tres meses de lesiones cutáneas dolorosas de leves a moderadas en la extremidad inferior derecha. El examen clínico reveló cuatro tumores en la tibia derecha, que exhibían crecimiento exofítico, apariencia verrugosa y ulceración. Se observaron dos nódulos subcutáneos. Se observó linfadenopatía inguinal agrandada. El examen histopatológico reveló un diagnóstico de melanoma. Este caso enfatiza la necesidad de una vigilancia integral y multidisciplinaria en regiones con datos limitados sobre melanoma que conducen a un subdiagnóstico de esta malignidad. Además, destaca la importancia de la prevención primaria como la protección solar en zonas tropicales para prevenir el melanoma cutáneo, abogando por aumentar la concienciación y las medidas preventivas.

Abstract Background: Pentalinon andrieuxii, is a medicinal plant used in Mayan folk medicine against leishmaniosis. It is an important source of terpenoids whose biosynthesis has been reported to be influenced by the ontogeny of the plant. Hypotheses: Flowering positively influences the production of urechitol A in Pentalinon andrieuxii. Studied species: Pentalinon andrieuxii (Müll. Arg.) B.F. Hansen & Wunderlin (Apocynaceae) Study site and dates: Mérida, Yucatán, Mexico, 2019 and 2020. Methods: Flowering was induced by exposing plants to temperatures above 32 °C. Plant tissue samples were collected before flowering induction and at full bloom and extracted with methanol. Analyses of the dichloromethane-soluble fraction of the crude extract by Gas Chromatography-Mass Spectrometry allowed the detection and quantification of urechitol A. Results: Flowering stage of P. andrieuxii has a positive influence on the production of urechitol A in the root of the plant, with the contents of the tri-nor-sesquiterpene increasing from a minimum of 43 to a maximum of 91 times during the flowering stage of plants, both transformed and wild type. Conclusions: P. andrieuxii perceives high temperatures as an important environmental cue to flower; flowering positively influences the production of urechitol A in the roots of the plant; production of the tri-nor-sesquiterpene is controlled by the plant.

Resumen Antecedentes: Pentalinon andrieuxii, es una planta medicinal usada en la medicina tradicional Maya contra la leishmaniosis. Es una fuente importante de terpenoides cuya biosíntesis se ha reportado que es influenciada por la ontogenia de la planta. Hipótesis: La floración influye positivamente en la producción de urechitol A en Pentalinon andrieuxii. Especie estudiada: Pentalinon andrieuxii (Müll. Arg.) B.F. Hansen & Wunderlin (Apocynaceae) Lugar de estudio y fechas: Mérida, Yucatán, México, 2019 y 2020. Métodos: La floración se indujo exponiendo las plantas a temperaturas superiores a 32 °C. Las muestras de tejido vegetal se colectaron antes de la inducción de la floración y en plena floración, y se extrajeron con metanol. El análisis por Cromatografía de Gases-Espectrometría de Masas de la fracción soluble en diclorometano del extracto crudo permitió la detección y cuantificación de urechitol A. Resultados: La floración de P. andrieuxii tiene una influencia positiva en la producción de urechitol A en la raíz de la planta, observándose aumentos en el contenido del tri-nor-sesquiterpeno desde un mínimo de 43 hasta un máximo de 91 veces durante la floración de las plantas, tanto en plantas transformadas como silvestres. Conclusiones: P. andrieuxii percibe a las altas temperaturas como una señal ambiental importante para florecer; la floración influencia positivamente la producción de urechitol A en la raíz; la producción del tri-nor-sesquiterpeno es controlada por la planta.

Abstract Objective: The objective of this study was to determine the prevalence of depression in ischemic post-stroke patients in a routine vascular clinic evaluation in Mexico. Methods: Consecutive patients with ischemic stroke between January 2017 and July 2018 were enrolled. National Institute of Health Stroke Scale and Hamilton scales were used to assess neurological deficit and depression, respectively. The mRs was used to evaluate the degree of dependence in daily activities and the Barthel Index was used to measure functional independence. Quality of life was assessed using the SF-36 questionnaire. Follow-up appointment was performed between 3 months and 2 years post-stroke. Results: Of the 162 patients who met study inclusion criteria, 93 were contacted at 2 years of follow-up. Fifty-six were men (60.2%) and 37 were women (29.8%), with an average age of 63.5. About 36.6% of participants met the criteria for depressive disorder. Conclusions: There is a high prevalence of depression among patients who have suffered ischemic stroke as assessed in a routine vascular clinic evaluation.

Resumen Objetivo: Determinar la prevalencia de depresión después de un ictus isquémico en la evaluación de rutina de una clínica vascular en México. Métodos: Se incluyeron pacientes consecutivos con ictus isquémico de enero 2017 a julio 2018. Se realizaron las escalas clínicas del NIH para ictus y la escala de Hamilton para evaluar su estado funcional y la depresión, respectivamente. La escala modificada de Rankin se utilizó para evaluar el grado de dependencia en las actividades de la vida diaria y el índice de Barthel para medir el grado de la independencia funcional. La calidad de vida se evaluó con el cuestionario SF-36. Se dio seguimiento entre los 3 meses y 2 años posteriores al ictus. Resultados: De los 162 pacientes que cumplieron los criterios de inclusión, se logró contactar a 93 pacientes en el seguimiento a 2 años. 56 hombres (60.2%) y 37 mujeres (29.8%), con un promedio de edad de 63.5 años. El 36.6% de los participantes cumplieron los criterios de depresión. Conclusión: Hay una alta prevalencia de depresión entre los pacientes que han sufrido un ictus isquémico al ser evaluados de rutina en una clínica neurovascular.

Abstract Objective: The objective of the study is to evaluate the chronic inflammatory demyelinating polyneuropathy (CIDP) clinical spectrum in a cohort of Mexican patients who benefit from social security. Methods: A retrospective study of CIDP patients from 2010 to 2021. We documented neurological deficits, electrophysiological and laboratory parameters, diagnostic characteristics, disease activity, and clinical outcomes. Results: 36 cases met the criteria of the EAN/PNS 2021, the mean age was 58.25 ± 10.10 years, 50% were women, and 69.4% used intravenous immunoglobulin (IVIG). Concerning functional status, 63.9% had an independent walk and 86.1% had mild weakness in manual skills. The analysis of functional status showed better results in the IVIG treatment group. Conclusions: Similar to previous literature reports, 50% of our patients had stable active disease. Considering the heterogeneity of the disease, more research about treatment initiation and long-term results is warranted.

Resumen Objetivo: Evaluar el espectro clínico de la polineuropatía desmielinizante inflamatoria crónica (PDIC) en una cohorte de pacientes mexicanos beneficiarios de la seguridad social. Métodos: Estudio retrospectivo de pacientes con PDIC durante el período 2010-2021. Documentamos déficits neurológicos, parámetros electrofisiológicos y de laboratorio, características de diagnóstico, actividad de la enfermedad y resultados clínicos. Resultados: 36 casos cumplieron con los criterios de la EAN/PNS 2021, la edad promedio fue de 58,25 ± 10,10 años, el 50% eran mujeres y el 69,4% utilizaban inmunoglobulina intravenosa. En cuanto al estado funcional, el 63,9% presentaba marcha independiente y el 86,1% presentaba debilidad leve en las habilidades manuales. El análisis del estado funcional mostró mejores resultados en el grupo de tratamiento con inmunoglobulinas intravenosa. Conclusiones: Al igual que en informes de la literatura, el 50% de nuestros pacientes tenían enfermedad activa estable. Teniendo en cuenta la heterogeneidad de la enfermedad, se justifica realizar más investigaciones sobre el tratamiento y los resultados a largo plazo.

Abstract PalmerNET is a Research Network (RED2022-134285-T), financed by the Agencia Española de Investigación, to fight against Amaranthus palmeri (A. palmeri) S. Wats., an invasive weed species established in Lleida, Huesca and Extremadura, with the ability to evolve herbicide resistance. Under the leadership of researcher Joel Torra, the Agrotechnology Research CERCA Centre (Agrotecnio) and the Universitat de Lleida (UdL) have started PalmerNET, where three other universities participate, namely the Universidad Pública de Navarra, the Universidad de Vigo and the Universitat Politècnica de València, and three other research institutes namely the Centro de Investigación y Tecnología Agraria de Aragón (CITA), the Instituto Nacional de Investigaciones Agrarias of Madrid (INIA-CSIC) and the Centro de Investigaciones Científicas y Tecnológicas de Extremadura (CICYTEX). A total of 25 people, including researchers, professors and PhD students, integrate PalmerNET. The net will promote the collaboration and research between these research groups to understand and contribute to containing the spread of this invasive weed, and disseminate the available knowledge regarding this worrying crop protection problem. During the period of the network PalmerNET (from 2023 to 2025), several activities will be undertaken, like technical seminars with the sector, periodic meetings of the net members, and the creation of a web page. This network will foster cooperation between the participating members to reach a global view of the problem and join research and action efforts.

Resumen PalmerNET es una Red de Investigación (RED2022-134285-T), financiada por la Agencia Española de Investigación, para combatir Amaranthus palmeri (A. palmeri) S. Wats., especie invasora establecida en Lleida, Huesca y Extremadura, con capacidad de generar resistencias a herbicidas. Bajo la dirección del investigador Joel Torra, la Fundación Centro de Investigación en Agrotecnología (Agrotecnio) y la Universitat de Lleida (UdL) han puesto en marcha PalmerNET, donde participan, además, otras tres universidades: la Universidad Pública de Navarra, la Universidad de Vigo y la Universitat Politècnica de València, y otros tres centros de investigación: el Centro de Investigación y Tecnología Agraria de Aragón (CITA), el Instituto Nacional de Investigaciones Agrarias de Madrid (INIA-CSIC) y el Centro de Investigaciones Científicas y Tecnológicas de Extremadura (CICYTEX). Un total de 25 personas, entre investigadores, profesores y estudiantes predoctorales, integran PalmerNET. La red promoverá la colaboración e investigación entre los grupos participantes para comprender y poder contener la expansión de la mala hierba y divulgar el concimiento disponible sobre este grave problema fitosanitario. Durante la vigencia de la red PalmerNET (de 2023 a 2025), se realizarán Jornadas Técnicas de divulgación, se actualizará la información mediante reuniones de los miembros y la creación de una página web. La red potenciará la cooperación entre los investigadores participantes para alcanzar una visión global del problema, uniendo esfuerzos de investigación y de actuación.

Resumo Relatos da medicina popular costumam atuar como referencial para o desenvolvimento de novos fármacos a partir de moléculas naturais com ações terapêuticas para doenças de alta gravidade e prevalência como o câncer. Bromelia antiacantha Bertol. é uma espécie da família Bromeliaceae, considerada uma planta alimentícia não convencional (PANC), encontrada nas regiões sul e centro-oeste do Brasil. Apesar do alto teor nutritivo e potencial farmacológico de seus frutos, poucos estudos científicos relatam suas ações biológicas. Desta forma, este estudo avalia o perfil fitoquímico de extratos aquoso e etanólico obtidos de frutos de B. antiacantha, bem como a sua possível ação antioxidante, antitumoral e citotóxica. O extrato aquoso apresentou compostos fenólicos e flavonoides, enquanto os extratos etanólicos apontam a presença de flavonóides e cumarina em sua composição, independente da região de coleta. O extrato etanólico demonstrou efeito antioxidante mais promissor do que o extrato aquoso e também induziu uma inibição significativa na viabilidade de células humanas de câncer cervical da linhagem SiHa. Além disso, o tratamento com ambos extratos não alterou a viabilidade de células não tumorais da linhagem de queratinócitos humanos imortalizados (HaCaT). Estes dados trazem novas informações sobre extratos obtidos de uma espécie vegetal nativa, comestível e já utilizada tradicionalmente, mas abrindo novas perspectivas quanto a possíveis aplicações terapêuticas.

Abstract Reports from popular medicine usually act as a basis for the development of new drugs from natural compounds with therapeutic actions for serious diseases and prevalence such as cancer. Bromelia antiacantha Bertol. is a species of the Bromeliaceae family, considered an unconventional food plant, found in the south and midwest regions of Brazil. Despite the high nutritional content and pharmacological potential of its fruits, few scientific studies report its biological actions. Thus, this study evaluates the phytochemical profile of aqueous and ethanol extracts obtained from B. antiacantha fruits, as well as their possible antioxidant, antitumor, and cytotoxic activities. The aqueous extract exhibited phenolic compounds and flavonoids, while ethanol extracts indicated the presence of flavonoids and coumarin in their composition, regardless of the region of collection. The ethanolic extract demonstrated a more promising antioxidant effect than the aqueous extract and also induced a significant inhibition in the viability of human cervical cancer cells of the SiHa strain. In addition, treatment with both extracts did not alter the viability of non-tumor cells of the immortalized human keratinocyte lineage (HaCaT). These results bring new data about extracts obtained from a native plant, edible and traditionally used in popular medicine, opening new perspectives for its possible therapeutic application.

Abstract Introduction : After the implementation of mitigation strategies during the COVID-19 pandemic, the incidence of respiratory viruses, including human coronaviruses (HCoV), experienced a significant decrease. The aim of this study is to characterize the epidemiology and clinical aspects of HCoV infections in ambulatory adults during COVID-19 pandemic times. Methods : descriptive, prospective, longitudinal study performed in a private hospital in La Plata, Buenos Aires, Argentina between November 2020 and October 2022; 458 outpatient adults with upper respiratory tract infections (URTI) were studied undergoing clinical and microbiological follow-up. Results : 44 (9.6%) subjects were positive by multiplex PCR for HCoV. 14 of them for 229E (31.8%), 13 for OC43 (29.5%), 11 for HKU-1 (25.1%) and 6 for NL63 (13.6%). A repeated PCR was positive for the same HCoV in 19 (57%) of 33 patients on day 3-5. No hospitalizations or deaths were reported. Discussion : Endemic HCoV caused a significant pro portion of URTI among outpatient adults during COVID- 19-related restrictions times. An alternating pattern of circulation between alfa-HCoV and beta-HCoV was observed.

Resumo Espécies do gênero Anastrepha causam prejuízos em diversas frutas e hortaliças nas regiões Neártica e Neotropical. Dentre essas espécies, muitas estão presentes no Brasil. Neste estudo, pesquisamos as espécies de Anastrepha existentes no Bioma Chaco brasileiro. Avaliamos possíveis influências do clima e da vegetação na ocorrência de Anastrepha spp. Para isso foram utilizadas armadilhas e proteína de milho hidrolisada como isca. As coletas foram realizadas em três composições florísticas distintas no bioma Chaco, desde áreas permanentemente secas até áreas inundadas. Identificamos Anastrepha fraterculus (Wiedemann, 1830), A. sororcula Zucchi 1979, A. undosa Stone 1942 e A. daciformis Bezzi 1909. O ambiente seco e inundado não influenciou a ocorrência de espécies de Anastrepha. A presença de múltiplos hospedeiros aumenta o número de espécies. A riqueza e abundância de espécies de Anastrepha são baixas no Chaco brasileiro quando comparadas a outros ambientes nativos. Neotropical Brasil estudo spp isca inundadas Wiedemann, Wiedemann (Wiedemann 1830, 1830 , 1830) 1979 194 1909 nativos 183 197 19 190 18 1

Abstract Species of the genus Anastrepha cause injuries to several fruits and vegetables in the Nearctic and Neotropical regions. Among these species, many are present in Brazil. In this study, we surveyed the existing Anastrepha species in the Brazilian Chaco Biome. We evaluated possible influences of climate and vegetation on the occurrence of Anastrepha spp. For this, we used traps and hydrolyzed protein as bait. Collections were carried out in three distinct floristic compositions in the Chaco biome, from permanently dry areas to flooded sites. We identified Anastrepha fraterculus (Wiedemann, 1830), A. sororcula Zucchi 1979, A. undosa Stone 1942, and A. daciformis Bezzi 1909. The dry and flooded environment did not influence the occurrence of Anastrepha species. The presence of multiple hosts increases the number of species. The richness and abundance of Anastrepha species are lower in the Brazilian Chaco than in other native environments. regions Brazil study Biome spp bait biome sites Wiedemann, Wiedemann (Wiedemann 1830, 1830 , 1830) A 1979 1942 1909 environments 183 197 194 190 18 19 1

Resumo O objetivo principal deste estudo foi avaliar as relações biométricas entre as espécies Bagre bagre, Lutjanus synagris e Nebris microps e seus otólitos. A relação entre o tamanho do otólito (comprimento e peso) e o tamanho do peixe (comprimento padrão e peso total) foi determinada através do modelo de regressão linear (y = a + bx). Para a descrição morfológica, os otólitos de três espécimes foram selecionados por classe de comprimento padrão (10mm). Os caracteres morfológicos analisados foram escolhidos de acordo com a literatura tradicional. Foram analisados trezentos e oito exemplares de B. bagre, 200 de L. synagris e 237 de N. microps. Durante todo o período de coleta, a fonte de captura dos indivíduos foi o município de Raposa. As correlações lineares para peixes e comprimento de otólitos para B. bagre foram 0,9129 e 0,9652, respectivamente. Para L. synagris, os coeficientes foram 0,8634 e 0,8672, enquanto para N. microps, 0,9597 e 0,8636, respectivamente. A classificação morfológica de L. synagris e N. microps é do tipo Saggita, e da espécie B. bagre é do tipo Lapillus. A partir dos resultados aqui apresentados é possível observar que os dados morfométricos e morfológicos dos otólitos podem servir de parâmetro para estimar a relação entre o peixe e o otólito em termos de sua biomassa e do comprimento de um indivíduo e de uma população. total y bx. bx . bx) 10mm. 10mm mm (10mm) tradicional B 20 L 23 N coleta Raposa 09129 0 9129 0,912 09652 9652 0,9652 respectivamente 08634 8634 0,863 08672 8672 0,8672 09597 9597 0,959 08636 8636 0,8636 Saggita Lapillus população (10mm 2 0912 912 0,91 0965 965 0,965 0863 863 0,86 0867 867 0,867 0959 959 0,95 091 91 0,9 096 96 0,96 086 86 0,8 095 95 09 9 0, 08 8

Abstract The main objective of this study was to evaluate the biometric relationships between the species Bagre bagre, Lutjanus synagris and Nebris microps and their otoliths. The relationship between the size of the otolith (length and weight) and the size of the fish (standard length and total weight) was determined using the linear regression model (y = a + bx). For the morphological description, the otoliths of three specimens were selected by standard length class (10mm). The morphological characters analyzed were chosen according to traditional literature. Three hundred eight specimens of B. bagre, 200 of L. synagris and 237 of N. microps were analyzed. Throughout the collection period, the source of the capture of individuals was the municipality of Raposa. The linear correlations for fish and otolith length for B. bagre were 0.9129 and 0.9652, respectively. For L. synagris, the coefficients were 0.8634 and 0.8672, while for N. microps, 0.9597 and 0.8636, respectively. The morphological classification of L. synagris and N. microps is of the Saggita type, and the B. bagre species is of the Lapillus type. From the data presented here, it is possible to observe that otolith morphometric and morphological data can serve as a parameter to estimate the relationship between the fish and the otolith in terms of its biomass and the length of an individual and a population. weight y bx. bx . bx) description 10mm. 10mm mm (10mm) literature B 20 L 23 N period Raposa 09129 0 9129 0.912 09652 9652 0.9652 respectively 08634 8634 0.863 08672 8672 0.8672 09597 9597 0.959 08636 8636 0.8636 type here population (10mm 2 0912 912 0.91 0965 965 0.965 0863 863 0.86 0867 867 0.867 0959 959 0.95 091 91 0.9 096 96 0.96 086 86 0.8 095 95 09 9 0. 08 8

Abstract Background: Arboviral diseases are a group of infectious diseases caused by viruses transmitted by arthropods, mainly mosquitoes. These diseases, such as those caused by the dengue (DENV), Zika (ZIKV), chikungunya (CHIKV), and yellow fever (YFV) viruses, have a significant impact worldwide. In this context, entomological surveillance plays a crucial role in the control and prevention of arboviruses by providing essential information on the presence, distribution, and activity of vector mosquitoes. Based on entomological surveillance, transovarian transmission provides information regarding the maintenance and dissemination of arboviruses. The objective of this study was to detect these arboviruses in Goiânia, Goiás, and analyze the occurrence of transovarian transmission. Methods: Aedes aegypti eggs were collected from different regions of Goiânia and cultivated under controlled laboratory conditions until the emergence of adult mosquitoes. Adult females were grouped into pools containing their heads and thoraxes. These pools were subsequently evaluated using reverse-transcription quantitative polymerase chain reaction (RT-qPCR) assay. Results: A total of 157 pools (N=1570) were analyzed, with two pools testing positive for CHIKV and one pool testing positive for ZIKV, indicating that the offspring resulting from transovarian transmission are potentially infectious. Conclusions: In summary, the demonstration of the vertical transmission mechanisms of CHIKV and ZIKV in A. aegypti serves as an alert to health authorities, as these diseases are still underreported, and their primary urban vector has likely acquired this capacity, contributing to the dissemination of these infections. Background arthropods mosquitoes DENV, DENV , (DENV) (ZIKV) CHIKV, (CHIKV) YFV (YFV worldwide context presence distribution Goiás Methods thoraxes reversetranscription reverse transcription RTqPCR RT qPCR (RT-qPCR assay Results 15 N=1570 N1570 N 1570 (N=1570 analyzed Conclusions summary authorities underreported capacity infections (DENV (ZIKV (CHIKV 1 N=157 N157 (N=157 N=15 N15 (N=15 N=1 N1 (N=1 N= (N= (N

SUMMARY Beckwith-Wiedemann syndrome (BWS) is a common genetic congenital disease characterized by somatic overgrowth and its broad clinical spectrum includes pre- and post-natal macrosomia, macroglossia, visceromegaly, increased risk of neonatal hypoglycemia, and development of embryonic tumors. BWS occurs due to genetic/epigenetic changes involving growth-regulating genes, located on region 11p15, with an important genotype-phenotype correlation. Congenital adrenal hyperplasia (CAH) comprises a spectrum of autosomal recessive diseases presenting a variety of clinical manifestations due to a deficiency in one of the enzymes involved in cortisol secretion. Early diagnosis based on newborn screening prevents the adrenal crisis and early infant death. However, high 17-hydroxyprogesterone (17-OHP) levels can occur in newborns or premature infants without CAH, in situations of stress due to maternal or neonatal factors. Here, we report new cases of false-positive diagnosis of 21-hydroxylase deficiency during newborn screening – two girls and one boy with BWS. Methylation-specific multiplex ligation-dependent probe amplification revealed a gain of methylation in the H19 differentially methylated region. Notably, all three cases showed a complete normalization of biochemical changes, highlighting the transient nature of these hormonal findings that imitate the classical form of CAH. This report sheds light on a new cause of false-positive 21-hydroxylase deficiency diagnosis during newborn screening: Beckwith-Wiedemann syndrome. BeckwithWiedemann Beckwith Wiedemann (BWS pre postnatal post natal macrosomia macroglossia visceromegaly hypoglycemia tumors geneticepigenetic epigenetic growthregulating growth regulating genes 11p15 p genotypephenotype genotype phenotype correlation CAH (CAH secretion death However 17hydroxyprogesterone hydroxyprogesterone 17 17OHP OHP (17-OHP factors Here falsepositive false positive 21hydroxylase hydroxylase 21 Methylationspecific Methylation specific ligationdependent ligation dependent H H1 Notably 11p1 1 2 11p

Nanoparticles of zein (NPZ) stabilized with surfactant polyvinyl alcohol (PVA), and nanoparticles of zein/PVA loaded with eugenol (NPZ-Eug) and the essential oil extracted from the clove (Syzygium aromaticum) (NPZ-OC) were produced by nanoprecipitation method. The extraction method of essential oil of the clove was carried out by the hydrodistillation technique, obtaining a content of 81.5% (m/m) of eugenol. The nanoparticle preparation method was optimized using a one-factor-at-a-time design of experiments, where different levels of variables (zein concentration, PVA concentration, and eugenol concentration) were explored. The best condition of synthesis was obtained with 0.3% (m/v) zein, 0.6% (m/v) PVA and 1.5 mg L-1 eugenol or essential oil of the clove, reaching NPs with acceptable propriety for stable nanoparticulate systems: size particles (NPZ = 113, NPZ-Eug = 229 and NPZ-OC = 279 nm), polydispersity index (PDI < 0.26), and zeta potential (≈ -30 mV). Loaded NPs showed encapsulation efficiency and drug-loading of 52 and 20% (NPZ-Eug), and 62, 27% (NPZ-OC), respectively. The quantification method of eugenol was validated by analytical parameters. Finally, all NPs produced exhibited good colloidal stability, confirming the effectiveness of PVA as a stabilizing agent, which had not been previously reported for these systems. The results obtained provide promising prospects for further investigations into its potential application as a bioinsecticide agent. NPZ PVA, , (PVA) zeinPVA NPZEug Eug (NPZ-Eug Syzygium aromaticum NPZOC OC (NPZ-OC technique 815 81 5 81.5 m/m mm m (m/m onefactoratatime one factor at time experiments concentration explored 03 0 3 0.3 m/v mv v (m/v 06 6 0.6 15 1 1. L1 L L- systems 113 22 27 nm, nm nm) PDI 0.26, 026 0.26 26 0.26) ≈ ( 30 -3 mV. mV . mV) drugloading drug loading 20 NPZEug, 62 NPZOC, respectively parameters Finally stability agent (PVA 8 81. 0. 11 2 02 0.2 -