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1.
Cytotoxic effects of crotoxin from Crotalus durissus terrificus snake in canine mammary tumor cell lines
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Pedro, Giovana
; Brasileiro, Felipe César da Silva
; Macedo, Jamile Mariano
; Soares, Andreimar Martins
; Mafra, Gabriel Caporale
; Alves, Carlos Eduardo Fonseca
; Laufer-Amorim, Renée
.
Journal of Venomous Animals and Toxins including Tropical Diseases
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Abstract Background: Mammary gland tumors are the most prevalent neoplasm in intact female dogs, and they are good natural models to study comparative oncology. Most canine mammary malignancies, as in women, are commonly refractory to conventional therapies and demand continuous new therapeutic approaches. Crotalus durissus terrificus, also called rattlesnake, has more than 60 different proteins in its venom with multiple pharmaceutical uses, such as antitumor, antiviral, and antimicrobial action. Crotoxin, a potent β-neurotoxin formed by the junction of two subunits, a basic subunit (CB-PLA2) and an acidic subunit (crotapotin), has already been reported to have anticancer properties in different types of cancers. Methods: In this work, we describe the cytotoxic potential of crotoxin and its subunits compared to doxorubicin (drug of choice) in two canine mammary carcinoma cell lines. Results: Crotoxin, CB-PLA2, crotalic venom, and doxorubicin decreased cell viability and the ability to migrate in a dose-dependent manner, and crotapotin did not present an antitumoral effect. For all compounds, the predominant cell death mechanism was apoptosis. In addition, crotoxin did not show toxicity in normal canine mammary gland cells. Conclusion: Therefore, this work showed that crotoxin and CB-PLA2 had cytotoxic activity, migration inhibition, and pro-apoptotic potential in canine mammary gland carcinoma cell lines, making their possible use in cancer research. Background dogs oncology malignancies women approaches terrificus rattlesnake 6 uses antitumor antiviral action Crotoxin βneurotoxin β neurotoxin CBPLA2 CBPLA CB PLA2 PLA (CB-PLA2 crotapotin, , (crotapotin) cancers Methods drug choice lines Results CBPLA2, PLA2, dosedependent dose dependent manner effect compounds apoptosis addition cells Conclusion Therefore CB-PLA activity inhibition proapoptotic pro apoptotic research (CB-PLA (crotapotin
2.
Forty years of a Postgraduate Program in Cardiology at a Brazilian public university: indicators of its graduates university
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Braga Filho, Carlos Eduardo
; Silva, Talita Dias da
; Silva, Claudia Joanete da
; Santos, Cleidivan Alves dos
; Rodrigues, Frank Menezes
; Barbosa, Adriano Henrique Pereira
; Caixeta, Adriano
; Izar, Maria Cristina de Oliveira
; Luna-Filho, Bráulio
; Fonseca, Francisco Antonio Helfenstein
; Tallo, Fernando Sabia
; Roever, Leonardo
; Caricati-Neto, Afonso
; Menezes-Rodrigues, Francisco Sandro
.
ABSTRACT Purpose: To evaluate the profile of graduates of the Postgraduate Program (PGP) in Cardiology of a public federal university, according to sociodemographic factors and professional trajectory. Methods: The variables were collected from databases from the observed institution and digital platforms. The analysis of differences between the various levels of degrees was carried out in three cohorts: the entire historical series (graduates from 1978–2021), the first 20 years (1978–1997) and the second 20 years (1998–2018). Results: The results demonstrated that most students from the PGP completed a PhD and are men over 30 years old, they came from public universities and the Southeast region. In the first 20 years, significant differences were observed in the distribution of masters and doctors working professionally at the institution analyzed, as well as in the age of the students. In the 20 years of the second half, there were differences between masters and PhD working professionally in the institution itself, as they came from private universities, they are women and PhD. Conclusions: The changes in the profile of masters and PhD that graduated from this PGP in cardiology reflect transformations that occurred in the job market and academy over the decades. Purpose (PGP university trajectory Methods platforms cohorts 1978–2021, 19782021 1978–2021 , 1978 2021 1978–2021) 2 1978–1997 19781997 1997 (1978–1997 1998–2018. 19982018 1998–2018 . 1998 2018 (1998–2018) Results 3 old region analyzed half itself Conclusions decades 1978202 1978–202 197 202 1978–199 1978199 199 (1978–199 1998201 1998–201 201 (1998–2018 197820 1978–20 19 1978–19 197819 (1978–19 199820 1998–20 (1998–201 19782 1978–2 1 1978–1 19781 (1978–1 19982 1998–2 (1998–20 1978– (1978– 1998– (1998–2 (1978 (1998– (197 (1998 (19 (199 (1 (
3.
Effects of ozone therapy applied topically, by bagging, or both on the healing of clean wounds induced in rat’s skin topically bagging rats rat s
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Frezza, Bárbara Di Martino
; Rahal, Sheila Canevese
; Santos, Ivan Felismino Charas dos
; Silva, Bruna Martins da
; Silva Junior, José Ivaldo de Siqueira
; Ferreira, Gustavo Manea
; Vettorato, Michel de Campos
; Fogaça, Jéssica Leite
; Tsunemi, Miriam Harumi
; Fonseca-Alves, Carlos Eduardo
; Pellizzon, Claudia Helena
; Rosa, Vinicius dos Santos
.
ABSTRACT Purpose: This study aimed to evaluate the effects of ozone therapy applied topically and/or by bagging on the healing of clean wounds induced in rat’s skin. Methods: One hundred and twenty male rats of about 16 weeks old was divided into five groups: G1) saline solution (0.9%); G2) sunflower oil; G3) ozonated sunflower oil; G4) ozone bagging; G5) association of ozonated sunflower oil and ozone bagging. The wounds were evaluated through macroscopic, morphometric, histopathologic, and tensile strength analyses. Results: Analysis among groups showed a lower percentage of wound contraction in G1 compared to G4 only in M7D. The tensile strength of the wounds showed differences among groups in the seventh (M7D) and the 14th (M14D) postoperative day, and among time points in G1 (M14D > M7D). The elongation of the wounds showed differences in G3 (M7D > M14D). Histological evaluation of the wounds showed significant change in bleeding, mixed to mononuclear infiltrate, congestion, and tissue disorganization for tissue organization between groups and time points. Conclusions: Ozone therapy applied topically and/or by bagging was not deleterious to the healing of clean wounds induced in rat’s skin, but ozone bagging showed the best contribution to the healing process. Purpose andor or rat s skin Methods 1 G 0.9% 09 0 9 (0.9%) G2 G5 macroscopic morphometric histopathologic analyses Results M7D MD M D th M14D day . M7D) M14D. M14D) bleeding infiltrate congestion Conclusions process 0.9 (0.9% 0. (0.9 (0. (0 (
4.
BRAZILIAN SOCIETY OF HEPATOLOGY UPDATED RECOMMENDATIONS FOR SYSTEMIC TREATMENT OF HEPATOCELLULAR CARCINOMA
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CHAGAS, Aline Lopes
; LEAL, Cassia Regina Guedes
; MELLO, Vivianne Barreto de
; BARROS, Fábio Marinho Do Rego
; BITTENCOURT, Paulo Lisboa
; MATTOS, Angelo A
; AROUCHA, Dayse
; FONSECA, Leonardo G da
; SILVA, Joyce Roma Lucas de
; DOTTORI, Mariana Fonseca
; TEIXEIRA, Rosangela
; MENDES, Liliana Sampaio Costa
; REZENDE, Rosamar Eulira Fontes
; FILGUEIRA, Norma Arteiro
; COUTINHO, Anelisa K
; ARAÚJO NETO, João Marcello de
; COELHO, Henrique Sergio Moraes
; PESSOA, Mario Guimarães
; CHEINQUER, Hugo
; PARISE, Edison Roberto
; FRANÇA, Alex
; ÁLVARES-DA-SILVA, Mário Reis
; CARRILHO, Flair José
; CORAL, Gabriela P
; PINTO, Paulo de Tarso Aparecida
; PEREIRA, Leila M M Beltrão
; PARANÁ, Raymundo
; ALVES, Rogério Camargo Pinheiro
; BRANDÃO-MELLO, Carlos Eduardo
.
RESUMO O carcinoma hepatocelular (CHC) é uma das principais causas de mortalidade relacionada a câncer no Brasil e no mundo. A Sociedade Brasileira de Hepatologia (SBH) publicou em 2020 a atualização das recomendações da SBH para o diagnóstico e tratamento do CHC. Desde então, novas evidências científicas sobre o tratamento sistêmico do CHC foram relatadas na literatura médica, incluindo novos medicamentos aprovados que não estavam disponíveis na época do último consenso, levando a diretoria da SBH a promover uma reunião monotemática on-line para discutir e rever as recomendações sobre o tratamento sistêmico do CHC. Um grupo de experts foi convidado para realizar uma revisão sistemática da literatura e apresentar uma atualização, baseada em evidências científicas, sobre cada tópico relacionado ao tratamento sistêmico e a apresentar os dados e recomendações resumidas durante a reunião. Todos os painelistas se reuniram para discutir os tópicos e elaborar as recomendações atualizadas. O presente documento é a versão final do manuscrito revisado, contendo as recomendações da SBH, e seu objetivo é auxiliar os profissionais de saúde, formuladores de políticas e planejadores no Brasil e na América Latina na tomada de decisões sobre o tratamento sistêmico de pacientes com CHC. (CHC mundo (SBH 202 então médica consenso online on line atualizadas revisado saúde 20 2
ABSTRACT Hepatocellular carcinoma (HCC) is the third most common cause of cancer-related mortality worldwide. The Brazilian Society of Hepatology (SBH) published in 2020 the updated recommendations for the diagnosis and treatment of HCC. Since then, new data have emerged in the literature, including new drugs approved for the systemic treatment of HCC that were not available at the time. The SBH board conducted an online single-topic meeting to discuss and review the recommendations on the systemic treatment of HCC. The invited experts were asked to conduct a systematic review of the literature on each topic related to systemic treatment and to present the summary data and recommendations during the meeting. All panelists gathered together for discussion of the topics and elaboration of the updated recommendations. The present document is the final version of the reviewed manuscript containing the recommendations of SBH and its aim is to assist healthcare professionals, policy-makers, and planners in Brazil and Latin America with systemic treatment decision-making of patients with HCC. (HCC cancerrelated cancer worldwide (SBH 202 then time singletopic single professionals policymakers, policymakers policy makers, makers policy-makers decisionmaking decision making 20 2
5.
Posicionamento Brasileiro sobre Síndrome da Quilomicronemia Familiar – 2023 202 20 2
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Izar, Maria Cristina de Oliveira
; Santos Filho, Raul Dias dos
; Assad, Marcelo Heitor Vieira
; Chagas, Antonio Carlos Palandri
; Toledo Júnior, Alceu de Oliveira
; Nogueira, Ana Cláudia Cavalcante
; Souto, Ana Cristina Carneiro Fernandes
; Lottenberg, Ana Maria
; Chacra, Ana Paula Marte
; Ferreira, Carlos Eduardo dos Santos
; Lourenço, Charles Marques
; Valerio, Cynthia Melissa
; Cintra, Dennys Esper
; Fonseca, Francisco Antonio Helfenstein
; Campana, Gustavo Aguiar
; Bianco, Henrique Tria
; Lima, Josivan Gomes de
; Castelo, Maria Helane Costa Gurgel
; Scartezini, Marileia
; Moretti, Miguel Antonio
; Barreto, Natasha Slhessarenko Fraife
; Maia, Rayana Elias
; Montenegro Junior, Renan Magalhães
; Alves, Renato Jorge
; Figueiredo, Roberta Marcondes Machado
; Fock, Rodrigo Ambrosio
; Martinez, Tânia Leme da Rocha
; Giraldez, Viviane Zorzanelli Rocha
.
6.
Inflamação Coronária Avaliada pela Atenuação de Gordura Pericoronária na Tomografia Computadorizada e Elevação de Citocinas em Usuários Jovens de Esteroides Anabólicos Androgênicos
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Souza, Francis Ribeiro de
; Rochitte, Carlos E.
; Silva, Douglas Carli
; Sampaio, Barbara
; Passarelli, Marisa
; Santos, Marcelo R. dos
; Fonseca, Guilherme W.
; Battaglia Filho, Antonio Carlos
; Correa, Kelly
; do Val, Renata Margarida
; Yonamine, Maurício
; Pereira, Rosa Maria R.
; Negrão, Carlos Eduardo
; Kalil-Filho, Roberto
; Alves, Maria Janieire de Nazaré Nunes
.
Resumo Fundamento O uso abusivo de esteroides anabólicos androgênicos (EAA) tem sido associado à doença arterial coronariana (DAC). A atenuação de gordura pericoronária (AGp) é um marcador de inflamação coronária, a qual exerce um papel chave no processo aterosclerótico. Objetivo Avaliar AGp e perfil inflamatório em usuários de EAA. Método Vinte indivíduos que realizavam treinamento de força, usuários de EAA (UEAA), 20 não usuários de EAA (NUEAA), e 10 indivíduos sedentários controle (SC) foram avaliados. Inflamação coronária foi avaliada por atenuação de gordura pericoronária média (AGPm) artéria coronária direita (ACD), artéria descendente anterior esquerda (ADA) e artéria circunflexa (ACX). Interleucina (IL)-1 (IL-1), IL-6, IL-10, e TNF-alfa foram avaliados por densidade ótica (DO) em um espectrofotômetro com um filtro de 450 nm. Um p<0,05 indicou significância estatística. Resultados Os UEAA apresentaram maior AGPm na ACD [-65,87 (70,51-60,70) vs. -78,07 (83,66-72,87) vs.-78,46 (85,41-71,99] unidades Hounsfield (HU), respectivamente, p<0,001) e AGPm na ADA [-71,47 (76,40-66,610 vs. -79,32 (84,37-74,59) vs. -82,52 (88,44-75,81) HU, respectivamente, p=0,006) em comparação aos NUEAA e CS. A AGPm na ACX não foi diferente entre os grupos UEAA, NUEAA e CS [-72,41 (77,17-70,37) vs. -80,13 (86,22-72,23) vs. -78,29 (80,63-72,29) HU, respectivamente, p=0,163). Em comparação aos NUEAA e aos CS, o grupo UEAA apresentaram maiores níveis de IL-1 [0,975 (0,847-1,250) vs. 0,437 (0,311-0,565) vs. 0,530 (0,402-0,780) DO, respectivamente, p=0,002), IL-6 [1,195 (0,947-1,405) vs. 0,427 (0,377-0,577) vs. 0,605 (0,332-0,950) DO, p=0,005) e IL-10 [1,145 (0,920-1,292) vs. 0,477 (0,382-0,591) vs. 0,340 (0,316-0,560) DO, p<0,001]. TNF-α não foi diferente entre os grupos UEAA, NUEAA e CS [0,520 (0,250-0,610) vs. 0,377 (0.261-0,548) vs. 0,350 (0,182-430)]. Conclusão Em comparação aos NUEAA e controles, os UEAA apresentam maior AGPm e maior perfil de citocinas inflamatórias sistêmicas, sugerindo que os EAA podem induzir aterosclerose por inflamação coronária e sistêmica. (EAA DAC. DAC . (DAC) (AGp aterosclerótico força , (UEAA) 2 NUEAA, (NUEAA) 1 SC (SC (AGPm ACD, (ACD) (ADA ACX. (ACX) IL1 IL (IL)- IL1, (IL-1) IL6, IL6 6, 6 IL10, IL10 10, TNFalfa TNF alfa DO (DO 45 nm p005 p 0 05 p<0,0 estatística 65,87 6587 65 87 [-65,8 70,5160,70 70516070 70,51 60,70 70 51 60 (70,51-60,70 vs 78,07 7807 78 07 -78,0 83,6672,87 83667287 83,66 72,87 83 66 72 (83,66-72,87 vs.78,46 vs7846 78,46 46 vs.-78,4 85,4171,99 85417199 85,41 71,99 85 41 71 99 (85,41-71,99 HU (HU) respectivamente p<0,001 p0001 001 71,47 7147 47 [-71,4 76,4066,610 764066610 76,40 66,610 76 40 610 (76,40-66,61 79,32 7932 79 32 -79,3 84,3774,59 84377459 84,37 74,59 84 37 74 59 (84,37-74,59 82,52 8252 82 52 -82,5 88,4475,81 88447581 88,44 75,81 88 44 75 81 (88,44-75,81 p=0,006 p0006 006 72,41 7241 [-72,4 77,1770,37 77177037 77,17 70,37 77 17 (77,17-70,37 80,13 8013 80 13 -80,1 86,2272,23 86227223 86,22 72,23 86 22 23 (86,22-72,23 78,29 7829 29 -78,2 80,6372,29 80637229 80,63 72,29 63 (80,63-72,29 p=0,163. p0163 p=0,163 163 p=0,163) IL- 0,975 0975 975 [0,97 0,8471,250 08471250 0,847 1,250 847 250 (0,847-1,250 0437 437 0,43 0,3110,565 03110565 0,311 0,565 311 565 (0,311-0,565 0530 530 0,53 0,4020,780 04020780 0,402 0,780 402 780 (0,402-0,780 p=0,002, p0002 p=0,002 002 p=0,002) 1,195 1195 195 [1,19 0,9471,405 09471405 0,947 1,405 947 405 (0,947-1,405 0427 427 0,42 0,3770,577 03770577 0,577 377 577 (0,377-0,577 0605 605 0,60 0,3320,950 03320950 0,332 0,950 332 950 (0,332-0,950 p=0,005 p0005 005 1,145 1145 145 [1,14 0,9201,292 09201292 0,920 1,292 920 292 (0,920-1,292 0477 477 0,47 0,3820,591 03820591 0,382 0,591 382 591 (0,382-0,591 0340 340 0,34 0,3160,560 03160560 0,316 0,560 316 560 (0,316-0,560 p<0,001. p<0,001] TNFα α 0,520 0520 520 [0,52 0,2500,610 02500610 0,250 0,610 (0,250-0,610 0377 0,37 0.2610,548 02610548 0.261 0,548 261 548 (0.261-0,548 0350 350 0,35 0,182430. 0182430 0,182 430 182 (0,182-430)] controles sistêmicas sistêmica (DAC (UEAA (NUEAA (ACD (ACX (IL) (IL-1 4 p00 p<0, 65,8 658 8 [-65, 5160 70,5160,7 7051607 7051 70,5 6070 60,7 7 5 (70,51-60,7 78,0 -78, 6672 83,6672,8 8366728 8366 83,6 7287 72,8 (83,66-72,8 vs.78,4 vs784 7846 78,4 vs.-78, 4171 85,4171,9 8541719 8541 85,4 7199 71,9 9 (85,41-71,9 (HU p<0,00 p000 00 71,4 714 [-71, 4066 76,4066,61 76406661 7640 76,4 66610 66,61 61 (76,40-66,6 79,3 793 3 -79, 3774 84,3774,5 8437745 8437 84,3 7459 74,5 (84,37-74,5 82,5 825 -82, 4475 88,4475,8 8844758 8844 88,4 7581 75,8 (88,44-75,8 p=0,00 72,4 724 [-72, 1770 77,1770,3 7717703 7717 77,1 7037 70,3 (77,17-70,3 80,1 801 -80, 2272 86,2272,2 8622722 8622 86,2 7223 72,2 (86,22-72,2 78,2 782 6372 80,6372,2 8063722 8063 80,6 7229 (80,63-72,2 p016 p=0,16 16 0,97 097 97 [0,9 8471 0,8471,25 0847125 0847 0,84 1250 1,25 25 (0,847-1,25 043 43 0,4 3110 0,3110,56 0311056 0311 0,31 0565 0,56 31 56 (0,311-0,56 053 53 0,5 4020 0,4020,78 0402078 0402 0,40 0780 0,78 (0,402-0,78 1,19 119 19 [1,1 9471 0,9471,40 0947140 0947 0,94 1405 1,40 94 (0,947-1,40 042 42 3770 0,3770,57 0377057 0577 0,57 57 (0,377-0,57 060 0,6 3320 0,3320,95 0332095 0332 0,33 0950 0,95 33 95 (0,332-0,95 1,14 114 14 9201 0,9201,29 0920129 0920 0,92 1292 1,29 92 (0,920-1,29 047 3820 0,3820,59 0382059 0382 0,38 0591 0,59 38 (0,382-0,59 034 34 0,3 3160 0,3160,56 0316056 0316 0560 (0,316-0,56 0,52 052 [0,5 2500 0,2500,61 0250061 0250 0,25 0610 0,61 (0,250-0,61 037 2610 0.2610,54 0261054 0261 0.26 0548 0,54 26 54 (0.261-0,54 035 35 182430 0,182430 018243 0182 0,18 18 (0,182-430) (IL (IL- p0 p<0 65, [-65 516 70,5160, 705160 705 70, 607 60, (70,51-60, 78, -78 667 83,6672, 836672 836 83, 728 72, (83,66-72, vs.78, vs78 784 vs.-78 417 85,4171, 854171 854 85, 719 71, (85,41-71, [-71 406 76,4066,6 7640666 764 76, 6661 66,6 (76,40-66, 79, -79 84,3774, 843774 843 84, 745 74, (84,37-74, 82, -82 447 88,4475, 884475 884 88, 758 75, (88,44-75, p=0,0 [-72 177 77,1770, 771770 771 77, 703 (77,17-70, 80, -80 227 86,2272, 862272 862 86, 722 (86,22-72, 637 80,6372, 806372 806 (80,63-72, p01 p=0,1 0,9 09 [0, 0,8471,2 084712 084 0,8 125 1,2 (0,847-1,2 04 0, 0,3110,5 031105 031 056 (0,311-0,5 0,4020,7 040207 040 078 0,7 (0,402-0,7 1,1 11 [1, 0,9471,4 094714 094 140 1,4 (0,947-1,4 0,3770,5 037705 057 (0,377-0,5 06 0,3320,9 033209 033 095 (0,332-0,9 0,9201,2 092012 092 129 (0,920-1,2 0,3820,5 038205 038 059 (0,382-0,5 03 0,3160,5 031605 (0,316-0,5 0,2500,6 025006 025 0,2 061 (0,250-0,6 0.2610,5 026105 026 0.2 054 (0.261-0,5 18243 0,18243 01824 018 0,1 (0,182-430 p< [-6 70,5160 70516 (70,51-60 -7 83,6672 83667 (83,66-72 vs.78 vs7 vs.-7 85,4171 85417 (85,41-71 [-7 76,4066, 764066 666 66, (76,40-66 84,3774 84377 (84,37-74 -8 88,4475 88447 (88,44-75 p=0, 77,1770 77177 (77,17-70 86,2272 86227 (86,22-72 80,6372 80637 (80,63-72 [0 0,8471, 08471 08 12 1, (0,847-1, 0,3110, 03110 (0,311-0, 0,4020, 04020 (0,402-0, [1 0,9471, 09471 (0,947-1, 0,3770, 03770 (0,377-0, 0,3320, 03320 (0,332-0, 0,9201, 09201 (0,920-1, 0,3820, 03820 (0,382-0, 0,3160, 03160 (0,316-0, 0,2500, 02500 02 (0,250-0, 0.2610, 02610 0. (0.261-0, 1824 0,1824 01 (0,182-43 [- 70,516 (70,51-6 - 83,667 (83,66-7 vs.7 vs.- 85,417 (85,41-7 76,4066 76406 (76,40-6 84,377 (84,37-7 88,447 (88,44-7 p=0 77,177 (77,17-7 86,227 (86,22-7 80,637 (80,63-7 [ 0,8471 (0,847-1 0,3110 (0,311-0 0,4020 (0,402-0 0,9471 (0,947-1 0,3770 (0,377-0 0,3320 (0,332-0 0,9201 (0,920-1 0,3820 (0,382-0 0,3160 (0,316-0 0,2500 (0,250-0 0.2610 (0.261-0 (0,182-4 (70,51- (83,66- (85,41- 76,406 (76,40- (84,37- (88,44- p= (77,17- (86,22- (80,63- (0,847- (0,311- (0,402- (0,947- (0,377- (0,332- (0,920- (0,382- (0,316- (0,250- (0.261- (0,182- (70,51 (83,66 (85,41 (76,40 (84,37 (88,44 (77,17 (86,22 (80,63 (0,847 (0,311 (0,402 (0,947 (0,377 (0,332 (0,920 (0,382 (0,316 (0,250 (0.261 (0,182 (70,5 (83,6 (85,4 (76,4 (84,3 (88,4 (77,1 (86,2 (80,6 (0,84 (0,31 (0,40 (0,94 (0,37 (0,33 (0,92 (0,38 (0,25 (0.26 (0,18 (70, (83, (85, (76, (84, (88, (77, (86, (80, (0,8 (0,3 (0,4 (0,9 (0,2 (0.2 (0,1 (70 (83 (85 (76 (84 (88 (77 (86 (80 (0, (0. (7 (8 (0 (
Abstract Background Anabolic androgenic steroid (AAS) abuse has been associated with coronary artery disease (CAD). Pericoronary fat attenuation (pFA) is a marker of coronary inflammation, which is key in the atherosclerotic process. Objective To evaluate pFA and inflammatory profile in AAS users. Methods Twenty strength-trained AAS users (AASU), 20 AAS nonusers (AASNU), and 10 sedentary controls (SC) were evaluated. Coronary inflammation was evaluated by mean pericoronary fat attenuation (mPFA) in the right coronary artery (RCA), left anterior descending coronary artery (LAD), and left circumflex (LCx). Interleukin (IL)-1 (IL-1), IL-6, IL-10, and TNF-alpha were evaluated by optical density (OD) in a spectrophotometer with a 450 nm filter. P<0.05 indicated statistical significance. Results AASU had higher mPFA in the RCA (-65.87 [70.51-60.70] vs. -78.07 [83.66-72.87] vs.-78.46 [85.41-71.99] Hounsfield Units (HU), respectively, p<0.001) and mPFA in the LAD (-71.47 [76.40-66.61] vs. -79.32 [84.37-74.59] vs. -82.52 [88.44-75.81] HU, respectively, p=0.006) compared with AASNU and SC. mPFA in the LCx was not different between AASU, AASNU, and SC (-72.41 [77.17-70.37] vs. -80.13 [86.22-72.23] vs. -78.29 [80.63-72.29] HU, respectively, p=0.163). AASU compared with AASNU and SC, had higher IL-1, (0.975 [0.847-1.250] vs. 0.437 [0.311-0.565] vs. 0.530 [0.402-0.780] OD, respectively, p=0.002), IL-6 (1.195 [0.947-1.405] vs. 0.427 [0.377-0.577] vs. 0.605 [0.332-0.950] OD, p=0.005) and IL-10 (1.145 [0.920-1.292] vs. 0.477 [0.382-0.591] vs. 0.340 [0.316-0.560] OD, p<0.001). TNF-α was not different between the AASU, AASNU, and SC groups (0.520 [0.250-0.610] vs. 0.377 [0.261-0.548] vs. 0.350 [0.182-430]), respectively. Conclusion Compared with ASSNU and controls, AASU have higher mPFA and higher systemic inflammatory cytokines profile suggesting that AAS may induce coronary atherosclerosis through coronary and systemic inflammation. (AAS CAD. CAD . (CAD) (pFA process strengthtrained strength trained , (AASU) 2 (AASNU) 1 (SC (mPFA RCA, (RCA) LAD, (LAD) LCx. (LCx) IL1 IL (IL)- IL1, (IL-1) IL6, IL6 6, 6 IL10, IL10 10, TNFalpha TNF alpha OD (OD 45 filter P005 P 0 05 P<0.0 significance 65.87 6587 65 87 (-65.8 70.5160.70 70516070 70.51 60.70 70 51 60 [70.51-60.70 vs 78.07 7807 78 07 -78.0 83.6672.87 83667287 83.66 72.87 83 66 72 [83.66-72.87 vs.78.46 vs7846 78.46 46 vs.-78.4 85.4171.99 85417199 85.41 71.99 85 41 71 99 [85.41-71.99 HU (HU) respectively p<0.001 p0001 p 001 71.47 7147 47 (-71.4 76.4066.61 76406661 76.40 66.61 76 40 61 [76.40-66.61 79.32 7932 79 32 -79.3 84.3774.59 84377459 84.37 74.59 84 37 74 59 [84.37-74.59 82.52 8252 82 52 -82.5 88.4475.81 88447581 88.44 75.81 88 44 75 81 [88.44-75.81 p=0.006 p0006 006 72.41 7241 (-72.4 77.1770.37 77177037 77.17 70.37 77 17 [77.17-70.37 80.13 8013 80 13 -80.1 86.2272.23 86227223 86.22 72.23 86 22 23 [86.22-72.23 78.29 7829 29 -78.2 80.6372.29 80637229 80.63 72.29 63 [80.63-72.29 p=0.163. p0163 p=0.163 163 p=0.163) 1, IL-1 0.975 0975 975 (0.97 0.8471.250 08471250 0.847 1.250 847 250 [0.847-1.250 0437 437 0.43 0.3110.565 03110565 0.311 0.565 311 565 [0.311-0.565 0530 530 0.53 0.4020.780 04020780 0.402 0.780 402 780 [0.402-0.780 p=0.002, p0002 p=0.002 002 p=0.002) IL- 1.195 1195 195 (1.19 0.9471.405 09471405 0.947 1.405 947 405 [0.947-1.405 0427 427 0.42 0.3770.577 03770577 0.577 377 577 [0.377-0.577 0605 605 0.60 0.3320.950 03320950 0.332 0.950 332 950 [0.332-0.950 p=0.005 p0005 005 1.145 1145 145 (1.14 0.9201.292 09201292 0.920 1.292 920 292 [0.920-1.292 0477 477 0.47 0.3820.591 03820591 0.382 0.591 382 591 [0.382-0.591 0340 340 0.34 0.3160.560 03160560 0.316 0.560 316 560 [0.316-0.560 p<0.001. TNFα α 0.520 0520 520 (0.52 0.2500.610 02500610 0.250 0.610 610 [0.250-0.610 0377 0.37 0.2610.548 02610548 0.261 0.548 261 548 [0.261-0.548 0350 350 0.35 0.182430, 0182430 0.182 430 182 [0.182-430]) (CAD (AASU (AASNU (RCA (LAD (LCx (IL) (IL-1 4 P00 P<0. 65.8 658 8 (-65. 5160 70.5160.7 7051607 7051 70.5 6070 60.7 7 5 [70.51-60.7 78.0 -78. 6672 83.6672.8 8366728 8366 83.6 7287 72.8 [83.66-72.8 vs.78.4 vs784 7846 78.4 vs.-78. 4171 85.4171.9 8541719 8541 85.4 7199 71.9 9 [85.41-71.9 (HU p<0.00 p000 00 71.4 714 (-71. 4066 76.4066.6 7640666 7640 76.4 6661 66.6 [76.40-66.6 79.3 793 3 -79. 3774 84.3774.5 8437745 8437 84.3 7459 74.5 [84.37-74.5 82.5 825 -82. 4475 88.4475.8 8844758 8844 88.4 7581 75.8 [88.44-75.8 p=0.00 72.4 724 (-72. 1770 77.1770.3 7717703 7717 77.1 7037 70.3 [77.17-70.3 80.1 801 -80. 2272 86.2272.2 8622722 8622 86.2 7223 72.2 [86.22-72.2 78.2 782 6372 80.6372.2 8063722 8063 80.6 7229 [80.63-72.2 p016 p=0.16 16 0.97 097 97 (0.9 8471 0.8471.25 0847125 0847 0.84 1250 1.25 25 [0.847-1.25 043 43 0.4 3110 0.3110.56 0311056 0311 0.31 0565 0.56 31 56 [0.311-0.56 053 53 0.5 4020 0.4020.78 0402078 0402 0.40 0780 0.78 [0.402-0.78 1.19 119 19 (1.1 9471 0.9471.40 0947140 0947 0.94 1405 1.40 94 [0.947-1.40 042 42 3770 0.3770.57 0377057 0577 0.57 57 [0.377-0.57 060 0.6 3320 0.3320.95 0332095 0332 0.33 0950 0.95 33 95 [0.332-0.95 1.14 114 14 9201 0.9201.29 0920129 0920 0.92 1292 1.29 92 [0.920-1.29 047 3820 0.3820.59 0382059 0382 0.38 0591 0.59 38 [0.382-0.59 034 34 0.3 3160 0.3160.56 0316056 0316 0560 [0.316-0.56 0.52 052 (0.5 2500 0.2500.61 0250061 0250 0.25 0610 0.61 [0.250-0.61 037 2610 0.2610.54 0261054 0261 0.26 0548 0.54 26 54 [0.261-0.54 035 35 182430 0.182430 018243 0182 0.18 18 [0.182-430] (IL (IL- P0 P<0 65. (-65 516 70.5160. 705160 705 70. 607 60. [70.51-60. 78. -78 667 83.6672. 836672 836 83. 728 72. [83.66-72. vs.78. vs78 784 vs.-78 417 85.4171. 854171 854 85. 719 71. [85.41-71. p<0.0 p00 (-71 406 76.4066. 764066 764 76. 666 66. [76.40-66. 79. -79 84.3774. 843774 843 84. 745 74. [84.37-74. 82. -82 447 88.4475. 884475 884 88. 758 75. [88.44-75. p=0.0 (-72 177 77.1770. 771770 771 77. 703 [77.17-70. 80. -80 227 86.2272. 862272 862 86. 722 [86.22-72. 637 80.6372. 806372 806 [80.63-72. p01 p=0.1 0.9 09 (0. 0.8471.2 084712 084 0.8 125 1.2 [0.847-1.2 04 0. 0.3110.5 031105 031 056 [0.311-0.5 0.4020.7 040207 040 078 0.7 [0.402-0.7 1.1 11 (1. 0.9471.4 094714 094 140 1.4 [0.947-1.4 0.3770.5 037705 057 [0.377-0.5 06 0.3320.9 033209 033 095 [0.332-0.9 0.9201.2 092012 092 129 [0.920-1.2 0.3820.5 038205 038 059 [0.382-0.5 03 0.3160.5 031605 [0.316-0.5 0.2500.6 025006 025 0.2 061 [0.250-0.6 0.2610.5 026105 026 054 [0.261-0.5 18243 0.18243 01824 018 0.1 [0.182-430 P< (-6 70.5160 70516 [70.51-60 -7 83.6672 83667 [83.66-72 vs.78 vs7 vs.-7 85.4171 85417 [85.41-71 p<0. p0 (-7 76.4066 76406 [76.40-66 84.3774 84377 [84.37-74 -8 88.4475 88447 [88.44-75 p=0. 77.1770 77177 [77.17-70 86.2272 86227 [86.22-72 80.6372 80637 [80.63-72 (0 0.8471. 08471 08 12 1. [0.847-1. 0.3110. 03110 [0.311-0. 0.4020. 04020 [0.402-0. (1 0.9471. 09471 [0.947-1. 0.3770. 03770 [0.377-0. 0.3320. 03320 [0.332-0. 0.9201. 09201 [0.920-1. 0.3820. 03820 [0.382-0. 0.3160. 03160 [0.316-0. 0.2500. 02500 02 [0.250-0. 0.2610. 02610 [0.261-0. 1824 0.1824 01 [0.182-43 (- 70.516 [70.51-6 - 83.667 [83.66-7 vs.7 vs.- 85.417 [85.41-7 p<0 76.406 [76.40-6 84.377 [84.37-7 88.447 [88.44-7 p=0 77.177 [77.17-7 86.227 [86.22-7 80.637 [80.63-7 ( 0.8471 [0.847-1 0.3110 [0.311-0 0.4020 [0.402-0 0.9471 [0.947-1 0.3770 [0.377-0 0.3320 [0.332-0 0.9201 [0.920-1 0.3820 [0.382-0 0.3160 [0.316-0 0.2500 [0.250-0 0.2610 [0.261-0 [0.182-4 [70.51- [83.66- [85.41- p< [76.40- [84.37- [88.44- p= [77.17- [86.22- [80.63- [0.847- [0.311- [0.402- [0.947- [0.377- [0.332- [0.920- [0.382- [0.316- [0.250- [0.261- [0.182- [70.51 [83.66 [85.41 [76.40 [84.37 [88.44 [77.17 [86.22 [80.63 [0.847 [0.311 [0.402 [0.947 [0.377 [0.332 [0.920 [0.382 [0.316 [0.250 [0.261 [0.182 [70.5 [83.6 [85.4 [76.4 [84.3 [88.4 [77.1 [86.2 [80.6 [0.84 [0.31 [0.40 [0.94 [0.37 [0.33 [0.92 [0.38 [0.25 [0.26 [0.18 [70. [83. [85. [76. [84. [88. [77. [86. [80. [0.8 [0.3 [0.4 [0.9 [0.2 [0.1 [70 [83 [85 [76 [84 [88 [77 [86 [80 [0. [7 [8 [0 [
7.
IMPACTO-MR: um estudo brasileiro de plataforma nacional para avaliar infecções e multirresistência em unidades de terapia intensiva IMPACTOMR IMPACTO MR IMPACTO-MR
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Tomazini, Bruno M
; Nassar Jr, Antonio Paulo
; Lisboa, Thiago Costa
; Azevedo, Luciano César Pontes de
; Veiga, Viviane Cordeiro
; Catarino, Daniela Ghidetti Mangas
; Fogazzi, Debora Vacaro
; Arns, Beatriz
; Piastrelli, Filipe Teixeira
; Dietrich, Camila
; Negrelli, Karina Leal
; Jesuíno, Isabella de Andrade
; Reis, Luiz Fernando Lima
; Mattos, Renata Rodrigues de
; Pinheiro, Carla Cristina Gomes
; Luz, Mariane Nascimento
; Spadoni, Clayse Carla da Silva
; Moro, Elisângela Emilene
; Bueno, Flávia Regina
; Sampaio, Camila Santana Justo Cintra
; Silva, Débora Patrício
; Baldassare, Franca Pellison
; Silva, Ana Cecilia Alcantara
; Veiga, Thabata
; Barbante, Leticia
; Lambauer, Marianne
; Campos, Viviane Bezerra
; Santos, Elton
; Santos, Renato Hideo Nakawaga
; Laranjeiras, Ligia Nasi
; Valeis, Nanci
; Santucci, Eliana
; Miranda, Tamiris Abait
; Patrocínio, Ana Cristina Lagoeiro do
; Carvalho, Andréa de
; Sousa, Eduvirgens Maria Couto de
; Sousa, Ancelmo Honorato Ferraz de
; Malheiro, Daniel Tavares
; Bezerra, Isabella Lott
; Rodrigues, Mirian Batista
; Malicia, Julliana Chicuta
; Silva, Sabrina Souza da
; Gimenes, Bruna dos Passos
; Sesin, Guilhermo Prates
; Zavascki, Alexandre Prehn
; Sganzerla, Daniel
; Medeiros, Gregory Saraiva
; Santos, Rosa da Rosa Minho dos
; Silva, Fernanda Kelly Romeiro
; Cheno, Maysa Yukari
; Abrahão, Carolinne Ferreira
; Oliveira Junior, Haliton Alves de
; Rocha, Leonardo Lima
; Nunes Neto, Pedro Aniceto
; Pereira, Valéria Chagas
; Paciência, Luis Eduardo Miranda
; Bueno, Elaine Silva
; Caser, Eliana Bernadete
; Ribeiro, Larissa Zuqui
; Fernandes, Caio Cesar Ferreira
; Garcia, Juliana Mazzei
; Silva, Vanildes de Fátima Fernandes
; Santos, Alisson Junior dos
; Machado, Flávia Ribeiro
; Souza, Maria Aparecida de
; Ferronato, Bianca Ramos
; Urbano, Hugo Corrêa de Andrade
; Moreira, Danielle Conceição Aparecida
; Souza-Dantas, Vicente Cés de
; Duarte, Diego Meireles
; Coelho, Juliana
; Figueiredo, Rodrigo Cruvinel
; Foreque, Fernanda
; Romano, Thiago Gomes
; Cubos, Daniel
; Spirale, Vladimir Miguel
; Nogueira, Roberta Schiavon
; Maia, Israel Silva
; Zandonai, Cassio Luis
; Lovato, Wilson José
; Cerantola, Rodrigo Barbosa
; Toledo, Tatiana Gozzi Pancev
; Tomba, Pablo Oscar
; Almeida, Joyce Ramos de
; Sanches, Luciana Coelho
; Pierini, Leticia
; Cunha, Mariana
; Sousa, Michelle Tereza
; Azevedo, Bruna
; Dal-Pizzol, Felipe
; Damasio, Danusa de Castro
; Bainy, Marina Peres
; Beduhn, Dagoberta Alves Vieira
; Jatobá, Joana D’Arc Vila Nova
; Moura, Maria Tereza Farias de
; Rego, Leila Rezegue de Moraes
; Silva, Adria Vanessa da
; Oliveira, Luana Pontes
; Sodré Filho, Eliene Sá
; Santos, Silvana Soares dos
; Neves, Itallo de Lima
; Leão, Vanessa Cristina de Aquino
; Paes, João Lucidio Lobato
; Silva, Marielle Cristina Mendes
; Oliveira, Cláudio Dornas de
; Santiago, Raquel Caldeira Brant
; Paranhos, Jorge Luiz da Rocha
; Wiermann, Iany Grinezia da Silva
; Pedroso, Durval Ferreira Fonseca
; Sawada, Priscilla Yoshiko
; Prestes, Rejane Martins
; Nascimento, Glícia Cardoso
; Grion, Cintia Magalhães Carvalho
; Carrilho, Claudia Maria Dantas de Maio
; Dantas, Roberta Lacerda Almeida de Miranda
; Silva, Eliane Pereira
; Silva, Antônio Carlos da
; Oliveira, Sheila Mara Bezerra de
; Golin, Nicole Alberti
; Tregnago, Rogerio
; Lima, Valéria Paes
; Silva, Kamilla Grasielle Nunes da
; Boschi, Emerson
; Buffon, Viviane
; Machado, André Sant’Ana
; Capeletti, Leticia
; Foernges, Rafael Botelho
; Carvalho, Andréia Schubert de
; Oliveira Junior, Lúcio Couto de
; Oliveira, Daniela Cunha de
; Silva, Everton Macêdo
; Ribeiro, Julival
; Pereira, Francielle Constantino
; Salgado, Fernanda Borges
; Deutschendorf, Caroline
; Silva, Cristofer Farias da
; Gobatto, Andre Luiz Nunes
; Oliveira, Carolaine Bomfim de
; Dracoulakis, Marianna Deway Andrade
; Alvaia, Natália Oliveira Santos
; Souza, Roberta Machado de
; Araújo, Larissa Liz Cardoso de
; Melo, Rodrigo Morel Vieira de
; Passos, Luiz Carlos Santana
; Vidal, Claudia Fernanda de Lacerda
; Rodrigues, Fernanda Lopes de Albuquerque
; Kurtz, Pedro
; Shinotsuka, Cássia Righy
; Tavares, Maria Brandão
; Santana, Igor das Virgens
; Gavinho, Luciana Macedo da Silva
; Nascimento, Alaís Brito
; Pereira, Adriano J
; Cavalcanti, Alexandre Biasi
.
RESUMO Objetivo: Descrever o IMPACTO-MR, um estudo brasileiro de plataforma nacional em unidades de terapia intensiva focado no impacto das infecções por bactérias multirresistentes relacionadas à assistência à saúde. Métodos: Descrevemos a plataforma IMPACTO-MR, seu desenvolvimento, critérios para seleção das unidades de terapia intensiva, caracterização da coleta de dados, objetivos e projetos de pesquisa futuros a serem realizados na plataforma. Resultados: Os dados principais foram coletados por meio do Epimed Monitor System® e consistiram em dados demográficos, dados de comorbidades, estado funcional, escores clínicos, diagnóstico de internação e diagnósticos secundários, dados laboratoriais, clínicos e microbiológicos e suporte de órgãos durante a internação na unidade de terapia intensiva, entre outros. De outubro de 2019 a dezembro de 2020, 33.983 pacientes de 51 unidades de terapia intensiva foram incluídos no banco de dados principal. Conclusão: A plataforma IMPACTO-MR é um banco de dados clínico brasileiro de unidades de terapia intensiva focado na pesquisa do impacto das infecções por bactérias multirresistentes relacionadas à assistência à saúde. Essa plataforma fornece dados para o desenvolvimento e pesquisa de unidades de terapia intensiva individuais e ensaios clínicos observacionais e prospectivos multicêntricos. Objetivo IMPACTOMR, IMPACTOMR IMPACTO MR, MR saúde Métodos Resultados System demográficos comorbidades funcional secundários laboratoriais outros 201 2020 33983 33 983 33.98 5 principal Conclusão multicêntricos 20 202 3398 3 98 33.9 2 339 9 33.
ABSTRACT Objective: To describe the IMPACTO-MR, a Brazilian nationwide intensive care unit platform study focused on the impact of health care-associated infections due to multidrug-resistant bacteria. Methods: We described the IMPACTO-MR platform, its development, criteria for intensive care unit selection, characterization of core data collection, objectives, and future research projects to be held within the platform. Results: The core data were collected using the Epimed Monitor System® and consisted of demographic data, comorbidity data, functional status, clinical scores, admission diagnosis and secondary diagnoses, laboratory, clinical, and microbiological data, and organ support during intensive care unit stay, among others. From October 2019 to December 2020, 33,983 patients from 51 intensive care units were included in the core database. Conclusion: The IMPACTO-MR platform is a nationwide Brazilian intensive care unit clinical database focused on researching the impact of health care-associated infections due to multidrug-resistant bacteria. This platform provides data for individual intensive care unit development and research and multicenter observational and prospective trials. Objective IMPACTOMR, IMPACTOMR IMPACTO MR, MR careassociated associated multidrugresistant multidrug resistant bacteria Methods selection collection objectives Results System status scores diagnoses laboratory stay others 201 2020 33983 33 983 33,98 5 Conclusion trials 20 202 3398 3 98 33,9 2 339 9 33,
8.
Epidemiological characterization of notified human brucellosis cases in Southern Brazil
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Bernardi, Fabricio
; Possa, Marina Gabriela
; Rossi, Camila Elizandra
; Benevenuto, Luíz Guilherme Dercore
; Nascif Junior, Iucif Abrão
; Jesus, Jacqueline de
; Oliveira, Barbara Cardoso de
; Zanelatto, Carla
; Sena, Joice Gama
; Fonseca-Alves, Carlos Eduardo
; Elias, Fabiana
.
Revista do Instituto de Medicina Tropical de São Paulo
- Journal Metrics
ABSTRACT Brucellosis is one of the most important and widespread bacterial zoonotic diseases worldwide, and it is transmitted to humans from various sources, including direct contact with infected animals and the ingestion of contaminated products, including unpasteurized milk. There are only a few epidemiological studies on said disease in humans in Western Santa Catarina, a region instantiated by agriculture. Thus, the objective of this study was to characterize the epidemiological aspects of human brucellosis reported in Western Santa Catarina from 2013 to 2018. The data were provided by the Epidemiological Surveillance Board (Diretoria de Vigilancia Epidemiologica). The frequency of the disease in humans and the epidemiological profile of confirmed human cases were evaluated. Cases that were screened positive and those that were confirmed and submitted to the therapeutic protocol were analyzed. During the study period, 3,671 people were tested, of which 12.34% were screened positive (453/ 3,671) and 3.40% were confirmed (125/3,671). The year with the highest number of people testing positive was 2015 (123 cases), and 2018 was the year with the highest number of confirmed cases (39 cases). Confirmed cases predominated in males (48.8%), self-declared white (22.4%), aged 20-59 years old (60%), with incomplete primary education (22.4%), of rural origin (59.2%), with occupational contact with cattle (64.8%), engaged in professions directly linked to agricultural and livestock activities (55.5%), and who reported consumption of unpasteurized dairy products (59.2%). No seasonal variation was observed in case numbers. The results demonstrated that brucellosis is an endemic disease in Western Santa Catarina.
9.
Práticas de ressuscitação volêmica em unidades de terapia intensiva brasileiras: uma análise secundária do estudo Fluid-TRIPS
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Freitas, Flavio Geraldo Rezende de
; Hammond, Naomi
; Li, Yang
; Azevedo, Luciano Cesar Pontes de
; Cavalcanti, Alexandre Biasi
; Taniguchi, Leandro
; Gobatto, André
; Japiassú, André Miguel
; Bafi, Antonio Tonete
; Mazza, Bruno Franco
; Noritomi, Danilo Teixeira
; Dal-Pizzol, Felipe
; Bozza, Fernando
; Salluh, Jorge Ibrahin Figueira
; Westphal, Glauco Adrieno
; Soares, Márcio
; Assunção, Murillo Santucci César de
; Lisboa, Thiago
; Lobo, Suzana Margarete Ajeje
; Barbosa, Achilles Rohlfs
; Ventura, Adriana Fonseca
; Souza, Ailson Faria de
; Silva, Alexandre Francisco
; Toledo, Alexandre
; Reis, Aline
; Cembranel, Allan
; Rea Neto, Alvaro
; Gut, Ana Lúcia
; Justo, Ana Patricia Pierre
; Santos, Ana Paula
; Albuquerque, André Campos D. de
; Scazufka, André
; Rodrigues, Antonio Babo
; Fernandino, Bruno Bonaccorsi
; Silva, Bruno Goncalves
; Vidal, Bruno Sarno
; Pinheiro, Bruno Valle
; Pinto, Bruno Vilela Costa
; Feijo, Carlos Augusto Ramos
; Abreu Filho, Carlos de
; Bosso, Carlos Eduardo da Costa Nunes
; Moreira, Carlos Eduardo Nassif
; Ramos, Carlos Henrique Ferreira
; Tavares, Carmen
; Arantes, Cidamaiá
; Grion, Cintia
; Mendes, Ciro Leite
; Kmohan, Claudio
; Piras, Claudio
; Castro, Cristine Pilati Pileggi
; Lins, Cyntia
; Beraldo, Daniel
; Fontes, Daniel
; Boni, Daniela
; Castiglioni, Débora
; Paisani, Denise de Moraes
; Pedroso, Durval Ferreira Fonseca
; Mattos, Ederson Roberto
; Brito Sobrinho, Edgar de
; Troncoso, Edgar M. V.
; Rodrigues Filho, Edison Moraes
; Nogueira, Eduardo Enrico Ferrari
; Ferreira, Eduardo Leme
; Pacheco, Eduardo Souza
; Jodar, Euzebio
; Ferreira, Evandro L. A.
; Araujo, Fabiana Fernandes de
; Trevisol, Fabiana Schuelter
; Amorim, Fábio Ferreira
; Giannini, Fabio Poianas
; Santos, Fabrício Primitivo Matos
; Buarque, Fátima
; Lima, Felipe Gallego
; Costa, Fernando Antonio Alvares da
; Sad, Fernando Cesar dos Anjos
; Aranha, Fernando G.
; Ganem, Fernando
; Callil, Flavio
; Costa Filho, Francisco Flávio
; Dall´Arto, Frederico Toledo Campo
; Moreno, Geovani
; Friedman, Gilberto
; Moralez, Giulliana Martines
; Silva, Guilherme Abdalla da
; Costa, Guilherme
; Cavalcanti, Guilherme Silva
; Cavalcanti, Guilherme Silva
; Betônico, Gustavo Navarro
; Betônico, Gustavo Navarro
; Reis, Hélder
; Araujo, Helia Beatriz N.
; Hortiz Júnior, Helio Anjos
; Guimaraes, Helio Penna
; Urbano, Hugo
; Maia, Israel
; Santiago Filho, Ivan Lopes
; Farhat Júnior, Jamil
; Alvarez, Janu Rangel
; Passos, Joel Tavares
; Paranhos, Jorge Eduardo da Rocha
; Marques, José Aurelio
; Moreira Filho, José Gonçalves
; Andrade, Jose Neto
; Sobrinho, José Onofre de C
; Bezerra, Jose Terceiro de Paiva
; Alves, Juliana Apolônio
; Ferreira, Juliana
; Gomes, Jussara
; Sato, Karina Midori
; Gerent, Karine
; Teixeira, Kathia Margarida Costa
; Conde, Katia Aparecida Pessoa
; Martins, Laércia Ferreira
; Figueirêdo, Lanese
; Rezegue, Leila
; Tcherniacovsk, Leonardo
; Ferraz, Leone Oliveira
; Cavalcante, Liane
; Rabelo, Ligia
; Miilher, Lilian
; Garcia, Lisiane
; Tannous, Luana
; Hajjar, Ludhmila Abrahão
; Paciência, Luís Eduardo Miranda
; Cruz Neto, Luiz Monteiro da
; Bley, Macia Valeria
; Sousa, Marcelo Ferreira
; Puga, Marcelo Lourencini
; Romano, Marcelo Luz Pereira
; Nobrega, Marciano
; Arbex, Marcio
; Rodrigues, Márcio Leite
; Guerreiro, Márcio Osório
; Rocha, Marcone
; Alves, Maria Angela Pangoni
; Alves, Maria Angela Pangoni
; Rosa, Maria Doroti
; Dias, Mariza D’Agostino
; Martins, Miquéias
; Oliveira, Mirella de
; Moretti, Miriane Melo Silveira
; Matsui, Mirna
; Messender, Octavio
; Santarém, Orlando Luís de Andrade
; Silveira, Patricio Júnior Henrique da
; Vassallo, Paula Frizera
; Antoniazzi, Paulo
; Gottardo, Paulo César
; Correia, Paulo
; Ferreira, Paulo
; Torres, Paulo
; Silva, Pedro Gabrile M. de Barros e
; Foernges, Rafael
; Gomes, Rafael
; Moraes, Rafael
; Nonato filho, Raimundo
; Borba, Renato Luis
; Gomes, Renato V
; Cordioli, Ricardo
; Lima, Ricardo
; López, Ricardo Pérez
; Gargioni, Ricardo Rath de Oliveira
; Rosenblat, Richard
; Souza, Roberta Machado de
; Almeida, Roberto
; Narciso, Roberto Camargo
; Marco, Roberto
; waltrick, Roberto
; Biondi, Rodrigo
; Figueiredo, Rodrigo
; Dutra, Rodrigo Santana
; Batista, Roseane
; Felipe, Rouge
; Franco, Rubens Sergio da Silva
; Houly, Sandra
; Faria, Sara Socorro
; Pinto, Sergio Felix
; Luzzi, Sergio
; Sant’ana, Sergio
; Fernandes, Sergio Sonego
; Yamada, Sérgio
; Zajac, Sérgio
; Vaz, Sidiner Mesquita
; Bezerra, Silvia Aparecida Bezerra
; Farhat, Tatiana Bueno Tardivo
; Santos, Thiago Martins
; Smith, Tiago
; Silva, Ulysses V. A.
; Damasceno, Valnei Bento
; Nobre, Vandack
; Dantas, Vicente Cés de Souza
; Irineu, Vivian Menezes
; Bogado, Viviane
; Nedel, Wagner
; Campos Filho, Walther
; Dantas, Weidson
; Viana, William
; Oliveira Filho, Wilson de
; Delgadinho, Wilson Martins
; Finfer, Simon
; Machado, Flavia Ribeiro
.
RESUMO Objetivo: Descrever as práticas de ressuscitação volêmica em unidades de terapia intensiva brasileiras e compará-las com as de outros países participantes do estudo Fluid-TRIPS. Métodos: Este foi um estudo observacional transversal, prospectivo e internacional, de uma amostra de conveniência de unidades de terapia intensiva de 27 países (inclusive o Brasil), com utilização da base de dados Fluid-TRIPS compilada em 2014. Descrevemos os padrões de ressuscitação volêmica utilizados no Brasil em comparação com os de outros países e identificamos os fatores associados com a escolha dos fluidos. Resultados: No dia do estudo, foram incluídos 3.214 pacientes do Brasil e 3.493 pacientes de outros países, dos quais, respectivamente, 16,1% e 26,8% (p < 0,001) receberam fluidos. A principal indicação para ressuscitação volêmica foi comprometimento da perfusão e/ou baixo débito cardíaco (Brasil 71,7% versus outros países 56,4%; p < 0,001). No Brasil, a percentagem de pacientes que receberam soluções cristaloides foi mais elevada (97,7% versus 76,8%; p < 0,001), e solução de cloreto de sódio a 0,9% foi o cristaloide mais comumente utilizado (62,5% versus 27,1%; p < 0,001). A análise multivariada sugeriu que os níveis de albumina se associaram com o uso tanto de cristaloides quanto de coloides, enquanto o tipo de prescritor dos fluidos se associou apenas com o uso de cristaloides. Conclusão: Nossos resultados sugerem que cristaloides são usados mais frequentemente do que coloides para ressuscitação no Brasil, e essa discrepância, em termos de frequências, é mais elevada do que em outros países. A solução de cloreto de sódio 0,9% foi o cristaloide mais frequentemente prescrito. Os níveis de albumina sérica e o tipo de prescritor de fluidos foram os fatores associados com a escolha de cristaloides ou coloides para a prescrição de fluidos.
Abstract Objective: To describe fluid resuscitation practices in Brazilian intensive care units and to compare them with those of other countries participating in the Fluid-TRIPS. Methods: This was a prospective, international, cross-sectional, observational study in a convenience sample of intensive care units in 27 countries (including Brazil) using the Fluid-TRIPS database compiled in 2014. We described the patterns of fluid resuscitation use in Brazil compared with those in other countries and identified the factors associated with fluid choice. Results: On the study day, 3,214 patients in Brazil and 3,493 patients in other countries were included, of whom 16.1% and 26.8% (p < 0.001) received fluids, respectively. The main indication for fluid resuscitation was impaired perfusion and/or low cardiac output (Brazil: 71.7% versus other countries: 56.4%, p < 0.001). In Brazil, the percentage of patients receiving crystalloid solutions was higher (97.7% versus 76.8%, p < 0.001), and 0.9% sodium chloride was the most commonly used crystalloid (62.5% versus 27.1%, p < 0.001). The multivariable analysis suggested that the albumin levels were associated with the use of both crystalloids and colloids, whereas the type of fluid prescriber was associated with crystalloid use only. Conclusion: Our results suggest that crystalloids are more frequently used than colloids for fluid resuscitation in Brazil, and this discrepancy in frequencies is higher than that in other countries. Sodium chloride (0.9%) was the crystalloid most commonly prescribed. Serum albumin levels and the type of fluid prescriber were the factors associated with the choice of crystalloids or colloids for fluid resuscitation.
https://doi.org/10.5935/0103-507x.20210028
273 downloads
10.
Elucidating tumor immunosurveillance and immunoediting: a comprehensive review
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Silva, Estela Vieira de Souza
; Nascente, Eduardo de Paula
; Miguel, Marina Pacheco
; Alves, Carlos Eduardo Fonseca
; Moura, Veridiana Maria Brianezi Dignani de
.
Resumo A ação do sistema imunológico contra as enfermidades neoplásicas tem se tornado uma das principais fontes de pesquisa na atualidade. As vias biológicas desse sistema são conhecidas por contribuir na limitação da progressão e na eliminação do tumor, e são delineadas por conceitos e mecanismos de imunovigilância e imunoedição. A imunovigilância é considerada o processo pelo qual o sistema imunológico reconhece e inibe o processo neoplásico. O conceito de imunoedição tem origem no sentido de que o sistema imune é capaz de moldar o perfil antigênico do tumor devido à pressão seletiva, baseada nas etapas de eliminação, equilíbrio e evasão tumoral. A resposta imunológica ocorre contra antígenos tumorais e modificações do microambiente tumoral, envolvendo diferentes componentes do sistema imune inato, como células T, células natural Killer, linfócitos B e macrófagos. Nesse sentido, conhecer esses conceitos e compreender seus respectivos mecanismos torna-se essencial na investigação de novas estratégias de prevenção e combate ao câncer. Dessa forma, esta revisão apresenta aspectos históricos e definições de imunovigilância e imunoedição tumoral, com ênfase em sua importância e aplicabilidade, assim como aos diferentes métodos utilizados em imunoterapia.
Abstract The action of the immune system against neoplastic diseases has become one of the main sources of research. The biological pathways of this system are known to contribute in limiting the progression and elimination of the tumor, and are delineated by concepts and mechanisms of immunosurveillance and immunoediting. Immunosurveillance is considered the process by which the immune system recognizes and inhibits the neoplastic process. The concept of immunoediting arises in the sense that immune system is able to shape the antigenic profile of the tumor due to selective pressure, based on the stages of tumor elimination, balance and evasion. The immune response occurs against tumor antigens and changes in the tumor microenvironment, involving different components of the innate immune system, such as T cells, natural Killer cells, B lymphocytes and macrophages. In this sense, knowing these concepts and understanding their respective mechanisms becomes essential in the investigation of new strategies for cancer prevention and cure. Thus, this review presents historical aspects and definitions of immunosurveillance and tumor immunoediting, with emphasis on its importance and applicability, such as on the different methods used in immunotherapy.
https://doi.org/10.1590/1809-6891v22e-68544
454 downloads
11.
Experimental transmission of Leishmania (Leishmania) amazonensis to immunosuppressed mice through the bite of Lutzomyia longipalpis (Diptera: Psychodidae) results in cutaneous leishmaniasis
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Silva, Rosa Cristina Ribeiro da
; Cruz, Léo Nava Piorsky Dominici
; Coutinho, João Manoel da Silva
; Fonseca-Alves, Carlos Eduardo
; Rebêlo, José Manuel Macário
; Pereira, Silma Regina Ferreira
.
Revista do Instituto de Medicina Tropical de São Paulo
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ABSTRACT Lutzomyia longipalpis is the natural vector of Leishmania (Leishmania) infantum, but it is also permissive for several Leishmania species that are related to cutaneous leishmaniasis (CL). Maranhao State (Northeast of Brazil) is endemic for CL and has the highest number of cases of diffuse cutaneous leishmaniasis (DCL) in the country. It is a rare disease associated with a defective immune response mainly caused by L. (L.) amazonensis. Additionally, the number of immunosuppressed patients infected with the etiologic agents of CL has increased, including regions in which the main vectors of CL are rare. Therefore, we investigated whether Lu. longipalpis is able to transmit L. (L.) amazonensis to uninfected and immunosuppressed mice, resulting in CL. For that, 291 sand flies took an initial blood meal in mice infected with L. (L.) amazonensis. Of these, 17 underwent a second feeding on uninfected and immunosuppressed mice (of which 58.8% were also positive for Leishmania according to data on the dissection of the intestine). After 27 days of infection, these mice exhibited leishmaniotic lesions. The occurrence of parasites on the animal’s skin was confirmed by limiting dilution and immunohistopathological analyses. Parasite DNA was also detected in paw lesions and inguinal lymph nodes. DNA sequencing confirmed the Leishmania species in insects and mice. The results confirmed the ability of Lu. longipalpis to become infected and experimentally transmit L. (L.) amazonensis to immunosuppressed rodents, resulting in leishmaniotic lesions. Our data open perspectives for the potential role of Lu. longipalpis in the epidemiology of urban cutaneous leishmaniasis, especially in immunosuppressed patients.
12.
Effect of low-level laser therapy on the membrane induced by the Masquelet technique at an orthotopic site in rabbits
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Coris, Jeniffer Gabriela Figueroa
; Rahal, Sheila Canevese
; Fonseca-Alves, Carlos Eduardo
; Mamprim, Maria Jaqueline
; Inamassu, Letícia Rocha
; Sampaio, Alexandre Botelho de Abreu
; Kano, Washington Takashi
; Mesquita, Luciane dos Reis
; Silva Junior, José Ivaldo de Siqueira
.
ABSTRACT Purpose: To evaluate the low-level laser therapy (LLLT) on the membrane induced by the Masquelet technique in rabbits. Methods: Twelve Norfolk rabbits at approximately 3 months of age were used. A 1-cm segmental defect was induced in both radii, which were filled with polymethylmethacrylate cylinder. LLLT was used postoperatively in the bone defect of one of the forelimbs every 48 hours for 15 days. Six rabbits were euthanatized on third and sixth postoperative weeks. Results: In both forelimbs, radiographs showed new bone growth from radius cut ends on the third postoperative week and more advanced stage on the sixth postoperative week. Ultrasound showed induced membrane one week after the surgery. Histologically, there were no significant differences in the semi-quantitative score of inflammation intensity, total number of blood vessels, bone metaplasia, and collagen. The average thicknesses were 2,050.17 and 1,451.96 μm for control membranes and 2,724.26 and 2,081.03 μm for irradiated membranes, respectively, on third and sixth postoperative weeks. Vascular endothelial growth factor A (VEGF-A) and platelet derived growth factor (PDGF) expression were present in the induced membranes of control and irradiated forelimbs, but there was no significant difference. Conclusions: Based on assessment methods, it was not possible to demonstrate the effect of LLLT on the induced membrane.
https://doi.org/10.1590/acb361003
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13.
An overview of the Sixth International Conference on the Comparative Biology of Monocotyledons - Monocots VI - Natal, Brazil, 2018
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Versieux, Leonardo M.
; Calvente, Alice
; Carvalho, Fernanda Antunes
; Fonseca, Carlos Roberto
; Lichston, Juliana Espada
; Voigt, Eduardo
; Fontes, Anderson Lopes
; Roque, Alan
; Williams, Joseph Hill
; Oliveira, Reyjane Patrícia de
; Viana, Pedro Lage
; Mercier, Helenice
; Palma-Silva, Clarisse
; Wendt, Tânia
; Temponi, Lívia Godinho
; Andrade, Ivanilza Moreira de
; Oriani, Aline
; Leandro, Thales D.
; Pessoa, Edlley Max
; Martinelli, Adriana Pinheiro
; André, Thiago
; Benko-Iseppon, Ana Maria
; Berg, Cássio van den
; Luz, Cynthia Fernandes Pinto da
; Prosdocimi, Francisco
; Jardim, Jomar G.
; Alves, Marccus
; Wanderley, Maria das Graças Lapa
; Forzza, Rafaela Campostrini
; Scatena, Vera Lucia
; Clark, Lynn G.
.
Resumo Neste artigo nós apresentamos uma visão geral dos resumos, programação científica e social, viagens de campo e informações acerca do número de inscritos, países participantes, patrocínios e temas tratados durante a Sexta Conferência Internacional sobre Biologia Comparada de Monocotiledôneas, realizada em Natal, Brasil, de 7 a 12 de outubro de 2018. Alguns comentários recebidos pelos organizadores e sugestões para os organizadores do próximo Monocots, na Costa Rica, também são fornecidos. Este artigo complementa os dados publicados no livro de resumos, disponível no Repositório Digital da UFRN.
Abstract In this paper we present an overview of the abstracts, scientific and social programs, field trips, and achievements in terms of participant number, represented countries, sponsorships, and themes treated during the Sixth International Conference on the Comparative Biology of Monocotyledons that took place in Natal, Brazil, October 7th -12th 2018. Some comments received by the organizers and a few suggestions for organizers of the next meeting, in Costa Rica, are also provided. The conference’s complete abstract book was published and provide additional information.
https://doi.org/10.1590/2175-7860202172035
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14.
Diretriz Brasileira de Cardio-oncologia – 2020
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Hajjar, Ludhmila Abrahão
; Costa, Isabela Bispo Santos da Silva da
; Lopes, Marcelo Antônio Cartaxo Queiroga
; Hoff, Paulo Marcelo Gehm
; Diz, Maria Del Pilar Estevez
; Fonseca, Silvia Moulin Ribeiro
; Bittar, Cristina Salvadori
; Rehder, Marília Harumi Higuchi dos Santos
; Rizk, Stephanie Itala
; Almeida, Dirceu Rodrigues
; Fernandes, Gustavo dos Santos
; Beck-da-Silva, Luís
; Campos, Carlos Augusto Homem de Magalhães
; Montera, Marcelo Westerlund
; Alves, Sílvia Marinho Martins
; Fukushima, Júlia Tizue
; Santos, Maria Verônica Câmara dos
; Negrão, Carlos Eduardo
; Silva, Thiago Liguori Feliciano da
; Ferreira, Silvia Moreira Ayub
; Malachias, Marcus Vinicius Bolivar
; Moreira, Maria da Consolação Vieira
; Valente Neto, Manuel Maria Ramos
; Fonseca, Veronica Cristina Quiroga
; Soeiro, Maria Carolina Feres de Almeida
; Alves, Juliana Barbosa Sobral
; Silva, Carolina Maria Pinto Domingues Carvalho
; Sbano, João
; Pavanello, Ricardo
; Pinto, Ibraim Masciarelli F.
; Simão, Antônio Felipe
; Dracoulakis, Marianna Deway Andrade
; Hoff, Ana Oliveira
; Assunção, Bruna Morhy Borges Leal
; Novis, Yana
; Testa, Laura
; Alencar Filho, Aristóteles Comte de
; Cruz, Cecília Beatriz Bittencourt Viana
; Pereira, Juliana
; Garcia, Diego Ribeiro
; Nomura, Cesar Higa
; Rochitte, Carlos Eduardo
; Macedo, Ariane Vieira Scarlatelli
; Marcatti, Patricia Tavares Felipe
; Mathias Junior, Wilson
; Wiermann, Evanius Garcia
; Val, Renata do
; Freitas, Helano
; Coutinho, Anelisa
; Mathias, Clarissa Maria de Cerqueira
; Vieira, Fernando Meton de Alencar Camara
; Sasse, André Deeke
; Rocha, Vanderson
; Ramires, José Antônio Franchini
; Kalil Filho, Roberto
.
https://doi.org/10.36660/abc.20201006
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15.
Epidemiological characterization of reported cases of brucellosis in cattle in the western region of the state of Santa Catarina, Brazil
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Bernardi, Fabricio
; Possa, Marina Gabriela
; Possa, Mariana
; Nascif Junior, Iucif Abrão
; Rossi, Camila Elizandra
; Alves, Carlos Eduardo Fonseca
; Elias, Fabiana
.
RESUMO: A brucelose é uma das zoonoses bacterianas mais importantes e difundidas no mundo. São poucos os estudos epidemiológicos sobre a doença em bovinos na região produtiva do Oeste de Santa Catarina. Desta forma, o objetivo deste estudo foi caracterizar os aspectos epidemiológicos da brucelose bovina notificados nesta região entre os anos de 2013 a 2018. Os dados sobre os bovinos foram fornecidos pela Companhia Integrada de Desenvolvimento Agrícola de Santa Catarina (CIDASC). Foram avaliadas as prevalências da doença nos bovinos e rebanhos, por meio de análise de tendência temporal e distribuição espacial. Os bovinos, cujo exame confirmatório mostrou positividade para brucelose, foram analisados quanto à localização (município) e o ano de confirmação do diagnóstico. No período de estudo (2013-2018) foram realizados 686.086 testes para brucelose, encontrando-se prevalência de 1,11% (7.586/686.086) de animais positivos, enquanto a prevalência de rebanhos positivos, dentre os 80.171 testados, foi de 0,95% (764/80171). As razões de chances de ocorrência de brucelose nos bovinos aumentaram significativamente nos anos de 2015 (OR= 1,55) e 2016 (OR= 1,97) e diminuíram em 2014 (OR= 0,47) e 2017 (OR= 0,97). A doença está presente nos bovinos em 61,07% (80/131) dos municípios em que foram realizados os testes. Os resultados demonstram que a brucelose é endêmica na região.
ABSTRACT: Brucellosis is one of the most important and widespread bacterial zoonoses in the world. There are few epidemiological studies on brucellosis in cattle in the productive region of western Santa Catarina. Thus, the objective of this study was to characterize the epidemiological aspects of bovine brucellosis reported in this region from 2013 to 2018. Cattle data were provided by the Integrated Company for Agricultural Development of Santa Catarina (Companhia Integrada de Desenvolvimento Agrícola de Santa Catarina - CIDASC). The prevalence of the disease was evaluated in cattle and herds by means of temporal trend analysis and spatial distribution. Bovines with tests showing positive for brucellosis were analyzed for location (municipality) and year of confirmation of diagnosis. In the study period (2013-2018), 686,086 tests were performed for brucellosis, with a prevalence rate of 1.11% (7,586/686,086). Among the 80,171 herds tested, 0.95% were found to be positive (764/80171). The odds ratio of brucellosis occurrence in cattle increased significantly in 2015 (OR = 1.55) and 2016 (OR = 1.97) and decreased in 2014 (OR = 0.47) and 2017 (OR = 0.97). The disease is present in cattle in 61.07% (80/131) of the municipalities where the tests were performed. Results showed that brucellosis is endemic in the region.
https://doi.org/10.1590/0103-8478cr20190678
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