Abstract Polyethylenimine (PEI) has been explored as an efficient non-viral system for delivering genes to cells; however, there were no protocols for its use in porcine fetal fibroblasts (PFF). Therefore, we compared different concentrations of FITC-PEI (0.625, 1.25, 2.5, 5, 10, 20, 40, or 80 µg/mL) and incubation times (30 min, 1 h, or 2 h). It was observed that the incubation time did not affect the internalization of the PEI-FITC and that 30 min was sufficient to capture the complex. The concentrations higher than 10 µg/mL could reach many marked PFF (>90%). Then, two PEI concentrations were tested, 10 or 40 µg/mL, combined with an N/P of 2 with the pmhyGENIE-5 for 30 min. The percentage of PFF-GFP positive was similar between the PEI concentrations in the evaluation time points (24 h, 48 h, and 72 h). However, 40 µg/mL caused higher membrane damage rates. Thus, it can be concluded that concentrations between 10 – 80 µg/ml of PEI promote high incorporation rates, even in periods as short as 30 minutes. Furthermore, it can be stated that the transfection condition used in Polyplexes 1 (10 µg/mL of PEI and 37.5 µg/mL of pmhyGENIE-5 for 30 min) efficiently produces genetically edited porcine fetal fibroblasts with low cell damage. (PEI nonviral non viral cells however PFF. . (PFF) Therefore FITCPEI FITC 0.625, 0625 0 625 (0.625 125 25 1.25 5 2.5 20 8 µgmL µg mL (3 h h. h) PEIFITC 3 complex >90%. 90 >90% (>90%) Then tested 4 NP N P pmhyGENIE5 pmhyGENIE pmhyGENIE- PFFGFP GFP 24 (2 7 However rates Thus µgml ml minutes Furthermore (1 375 37 37. (PFF 0.625 062 62 (0.62 12 1.2 2. ( 9 >90 (>90% 0.62 06 6 (0.6 1. >9 (>90 0.6 (0. > (>9 0. (0 (>

Abstract Salmonella enterica serovar Heidelberg (SH), often isolated from broiler chicken samples, damages the entire production chain due to its high resistance in the environment. The search for sustainable disinfectant agents has intensified, focusing on the action of essential oils. This work's objective was to evaluate the in vitro, effect of Thymus vulgaris essential oil (TEO) and thymol in SH isolated from broilers, and the cytotoxicity in animal cells. Four different concentrations of TEO (0.1%, 0.2%, 0.4%, and 0.8%, (v/v)) were used against five SH isolates obtained from broilers and ATCC 8326. Thymol was evaluated at the concentrations of 0.023%, 0.047%, 0.071%, and 0.094% (v/v). In vitro antibacterial assays were performed by quantifying viable planktonic cells in the broth microdilution test. The MTT technique was used to assess the cytotoxicity in IEC-6 intestinal cells and NCTC fibroblasts. IC50 was assessed testing the concentrations of 0.0625%, 0.125%, 0.25%, and 0.5% of TEO and thymol in 24 hours. The bacterial activity was observed from the 0.2% concentration of TEO since no colony-forming units were observed. Thymol in the concentration of 0.094% controlled 83.33% of the bacteria. TEO presented an IC50 of 0.14% and 1.22%, while the IC50 for thymol was 0.068% and 0.001% for the IEC-6 and NCTC cells, respectively. On the other hand, TEO showed low cytotoxicity in fibroblasts and a potential action capacity to eliminate SH strains in vitro. SH, , (SH) samples environment intensified oils works work s (TEO 0.1%, 01 0 1 (0.1% 02 2 04 4 0.4% 08 8 0.8% v/v vv v (v/v) 8326 0023 023 0.023% 0047 047 0.047% 0071 071 0.071% 0094 094 0.094 v/v. . test IEC6 IEC 6 IEC- IC IC5 00625 0625 0.0625% 0125 125 0.125% 025 25 0.25% 05 5 0.5 hours 0.2 colonyforming colony forming 8333 83 33 83.33 bacteria 014 14 0.14 122 22 1.22% 0068 068 0.068 0001 001 0.001 respectively hand (SH 0.1% (0.1 0.4 0.8 (v/v 832 002 0.023 004 0.047 007 07 0.071 009 09 0.09 0062 062 0.0625 012 12 0.125 0.25 0. 833 3 83.3 0.1 1.22 006 06 0.06 000 00 0.00 (0. 0.02 0.04 0.07 0.0 0.062 0.12 83. 1.2 (0 1. (

Analisa a influência dos grupos terapêuticos em Centro de Atenção Psicossocial entre usuários com dependência de cocaína/crack, em Fortaleza - Ceará. Estudo descritivo e retrospectivo, entre 2009 e 2010. Realizou-se uma análise bivariada entre as variáveis dependentes e independentes. Os grupos terapêuticos foram influenciados pela profissão, por abandono de tratamento, drogas ilícitas, número de consultas e prescrições. Observa-se a necessidade de elaborar estratégias para melhorar as ações preventivas comunitárias, visando a aumentar a adesão dos dependentes de cocaína/crack às atividades terapêuticas, conforme a legislação vigente de saúde mental.

It analyzes the influence of the therapeutic groups at Psychosocial Care Center among users with cocaine/crack dependence, Fortaleza - Ceará. Descriptive and retrospective study between 2009 and 2010. A bivariate analysis between the dependent and independent variables was performed. The therapeutic groups were influenced by the occupation, treatment abandonment, illegal drugs, number of visits to the doctors and prescriptions. It is observed the necessity to elaborate strategies in order to improve communitarian preventive actions, aiming to increase the cocaine/crack dependents adherence to the therapeutic activities, in accordance to the current mental health legislation.

Analisa a influência dos grupos terapêuticos em Centro de Atenção Psicossocial entre usuários com dependência de cocaína/crack, em Fortaleza - Ceará. Estudo descritivo e retrospectivo, entre 2009 e 2010. Realizou-se uma análise bivariada entre as variáveis dependentes e independentes. Os grupos terapêuticos foram influenciados pela profissão, por abandono de tratamento, drogas ilícitas, número de consultas e prescrições. Observa-se a necessidade de elaborar estratégias para melhorar as ações preventivas comunitárias, visando a aumentar a adesão dos dependentes de cocaína/crack às atividades terapêuticas, conforme a legislação vigente de saúde mental.

It analyzes the influence of the therapeutic groups at Psychosocial Care Center among users with cocaine/crack dependence, Fortaleza - Ceará. Descriptive and retrospective study between 2009 and 2010. A bivariate analysis between the dependent and independent variables was performed. The therapeutic groups were influenced by the occupation, treatment abandonment, illegal drugs, number of visits to the doctors and prescriptions. It is observed the necessity to elaborate strategies in order to improve communitarian preventive actions, aiming to increase the cocaine/crack dependents adherence to the therapeutic activities, in accordance to the current mental health legislation.

In echocardiographic evaluation of patients with aortic stenosis (AS), prospective studies have demonstrated that left ventricular stoke work loss index (LVSWLI) provide a more clinical efficacy than calculate of aortic valve area (AVA) by continuity equation to estimate severity of stenosis. The aim of this study was assess in our population of patients with AS the correlation between LVSWLI and AVA in regard to severity. Material and methods: Forty nine patients with moderate and severe AS were evaluated by transthoracic echocardiography. Grades of AS were assessed by transaortic flow velocity (Vmax) and mean aortic transvalvular gradient (ΔP). AVA and LVSWLI were calculated and Pearson's and Spearman's correlation coefficients between both methods were assessed. Significance level was set at <0.05. Results: The age of the patients was 66 ± 13 (31-84 years). Thirty four (69%) patients had severe AS and 15 (31%) moderate AS. The Pearson's correlation coefficient between LVSWLI and AVA was 0.79 (p < 0.04) and between LVSWLI and ΔP was 0.90 (p < 0.03). The Spearman's correlation coefficient between LVSWLI and symptomatic status was 0.70 (rho = 0.70, p < 0.003). Conclusions: In patients with moderate and severe AS, the correlation between LVSWLI and ΔP is higher than correlation between LVSWLI and AVA. Moreover LVSWLI has a higher correlation with presence of symptoms than AVA.

En la evaluación ecocardiográfica del paciente con estenosis valvular aórtica (EA), estudios prospectivos han demostrado que el índice de pérdida de trabajo-latido ventricular izquierdo (IPTLVI) tiene mayor eficacia clínica que el cálculo del área valvular aórtica (AVA) por ecuación de la continuidad para la valoración de la severidad. El objetivo del presente estudio fue determinar, en nuestra población de pacientes con EA, la correlación entre el IPTLVI y el AVA obtenida por ecuación de continuidad en la evaluación de la severidad. Material y métodos: Fueron evaluados por ecocardiografía transtorácica 49 pacientes con EAde grados moderado y severo determinados con base en los valores de velocidad máxima de flujo transaórtico (Vmáx) y gradiente transvalvular aórtico medio (ΔP). Se les calcularon tanto el AVA por ecuación de continuidad, como el IPTLVI y fueron determinados los coeficientes de correlación de Pearson y Spearman entre ambos métodos evaluatorios. La significancia estadística fue considerada con p < 0.05. Resultados: La edad de los pacientes incluidos fue de 66 ± 13 (31-84 años). En 34 (69%) pacientes la EAfue severa y en 15 (31%) moderada. El coeficiente de correlación de Pearson entre el IPTLVI y el AVA fue de 0.79 (p < 0.04), mientras que entre el IPTLVI y el ΔP fue de 0.90 (p < 0.03). El coeficiente de correlación de Spearman entre el IPTLVI y estatus sintomático fue de 0.70 (rho = 0.70, p < 0.003). Conclusiones: En pacientes con EA de grados moderado y severo el IPTLVI guarda correlación mayor con el ΔP que con el AVA. Además, este índice tiene mayor correlación con la presencia de síntomas que el AVA.