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ABSTRACT Vesicular stomatitis virus is the causative agent of the vesicular stomatitis disease, which mainly affects cattle, swine, and horses causing significant economic losses. Many other animal species, including humans, can be also affected by the disease. Up to now, the epidemiology of vesicular stomatitis disease is not well understood. Previous epidemiological studies in Central America described the existence of an association between the presence of vesicular stomatitis New Jersey virus antibodies and environmental risk factors such as mean annual rainfall, temperature, and elevation. Additionally, molecular epidemiology studies revealed that the phylogenetic relationship between vesicular stomatitis New Jersey virus isolates is more related to ecological factors than temporal factors. We have performed a genetic analysis of vesicular stomatitis virus samples isolated from animals in different geographical regions in Venezuela. We sequenced the hypervariable region of the phosphoprotein gene of 51 samples collected over eight years. The virus was found in a wide range of environmental conditions, at high and low altitudes and with variable levels of precipitation and temperatures. In contrast with previous reports, our phylogenetic analysis showed that viruses from different ecological regions did not present significant genetic differences. cattle swine losses species humans now understood rainfall temperature elevation Additionally Venezuela 5 years conditions temperatures reports differences

https://doi.org/10.1590/1808-1657000132023

Asociación Mexicana de Cirugía General, A.C. Programa de Recuperación Quirúrgica Mejorada. Cirugía endocrina tiroidea
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Pérez-Soto, Rafael Humberto

; Clemente-Gutiérrez, Uriel

; Alvarado-Bachmann, Raúl

; Basurto-Kuba, Erich Otto Paul

; Domínguez-Fonseca, Claudia Beatriz

; Barajas-Fregoso, Elpidio Manuel

; España-Gómez, María Nayví

; Hurtado-López, Luis Mauricio

; Medina-Escobedo, Sandra Gabriela

; Montalvo-Hernández, Jorge

; Pantoja-Millán, Juan Pablo

; Piscil-Salazar, Marco Antonio

; Ponce de León-Ballesteros, Guillermo

; Romero-Talamás, Héctor

; Sierra-Salazar, Mauricio

; Arch-Ferrer, Jorge Emilio

; Chávez-Tostado, Karla Verónica

; Rivera-Robledo, Carlos Gustavo

; Garay-Lechuga, Daniel

; Stoopen-Margáin, Enrique

; Fajardo-Cevallo, Rafael Enrique

; Ordóñez-Ortega, Samuel

; Velázquez-Fernández, David

Cirujano general

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sep 2023, Volume 45 N. 3 Pages 138 - 151

Abstract: En Es | Text: En Es | PDF: En | PDF: Es

Abstract: The main objective of the fast postoperative recovery strategies in surgery is to provide efficient treatment based on the best available scientific evidence to shorten the postoperative recovery of patients, decrease the incidence of complications associated with hospitalization and surgical intervention; and therefore, reduce hospital costs. In our country, more than 50% of thyroidectomies are performed by low-volume surgeons, fact that has already been shown to be a risk factor associated with increased rate of postsurgical complications such as transient or permanent hypoparathyroidism or vocal cord paralysis. Understanding that achieving the availability of high-volume surgical human resources throughout the national territory is a non-viable objective in the short term, despite the efforts to train high specialists in endocrine surgery, one of the initiatives proposed by the Mexican Association of General Surgery, A.C., is to provide a series of recommendations to guide national surgeons for achieving better surgical results, with the unique objective of increasing the quality of care of the Mexican population. Until now, in Mexico there was no established recovery protocols for patients undergoing thyroid surgery. The purpose of this work was to create a series of recommendations for the preoperative, intraoperative, and postoperative periods, to improve the outcomes of patients undergoing thyroidectomy. All these recommendations are based on the best scientific evidence available and are aimed to general surgeons with both high and low thyroidectomy volume.

Resumen: Los programas de recuperación mejorada en cirugía tienen como principal objetivo brindar un tratamiento eficiente basado en la mejor evidencia científica con la finalidad de acortar el tiempo de recuperación postoperatoria de los pacientes, disminuir la incidencia de complicaciones inherentes a la hospitalización y tratamiento quirúrgico; y por consecuencia reducir los costos hospitalarios. En nuestro país, más de 50% de las tiroidectomías son realizadas por cirujanos de bajo volumen, lo que ya se ha demostrado que incrementa la posibilidad de complicaciones postquirúrgicas, tales como hipoparatiroidismo y parálisis cordal transitoria o permanente. En el entendimiento de que alcanzar la disponibilidad de recursos humanos quirúrgicos de alto volumen en todo el territorio nacional es un objetivo no viable a corto plazo, a pesar de los esfuerzos de formar altos especialistas en cirugía endocrina, una de las iniciativas de la Asociación Mexicana de Cirugía General, A.C., es la de emitir una serie de recomendaciones que apoyen a los cirujanos nacionales a tener mejores resultados quirúrgicos, con el objetivo único de incrementar la calidad de atención de la población mexicana. Hasta la fecha actual, en México no existía, un protocolo establecido para los pacientes que son intervenidos de cirugía tiroidea. El presente trabajo tuvo como finalidad realizar un consenso de expertos para emitir recomendaciones en el periodo preoperatorio, intraoperatorio y postoperatorio para mejorar los desenlaces de los pacientes intervenidos de tiroidectomía. Todas estas recomendaciones basadas en la mejor evidencia científica disponible y orientadas a cirujanos tanto de alto como de bajo volumen quirúrgico en cuanto a tiroidectomía se refiere.

https://doi.org/10.35366/112923

A retrospective multicenter study of oral and maxillofacial lesions in older people
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CUNHA, John Lennon Silva

; CAVALCANTE, Israel Leal

; RODRIGUES, Ana Beatriz Rocha

; CATÃO, Niely Enetice de Sousa

; CRUZ, Vitória Maria Sousa

; TURATTI, Eveline

; CAVALCANTE, Roberta Barroso

; FONSECA, Felipe Paiva

; GORDÓN-NÚÑEZ, Manuel Antonio

; ALVES, Pollianna Muniz

; NONAKA, Cassiano Francisco Weege

; ANDRADE, Bruno Augusto Benevenuto de

Brazilian Oral Research

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2023, Volume 37 elocation e098

Abstract: En | Text: En | PDF: En

Abstract Few studies on the distribution of oral diseases in older people are available in the literature. This study aimed to investigate the prevalence and demographic characteristics of oral and maxillofacial lesions in geriatric patients (age ≥ 60 years). A retrospective descriptive cross-sectional study was performed. Biopsy records were obtained from archives of three Brazilian oral pathology centers over a 20-year period. Data on sex, age, anatomical site, skin color, and histopathological diagnosis were collected and analyzed. Pearson’s chi-square test was used to evaluate differences in the frequency of the different oral and maxillofacial lesion groups. A total of 7,476 biopsy records of older patients were analyzed. Most cases were diagnosed in patients aged 60 to 69 years (n = 4,487; 60.0%). Females were more affected (n = 4,403; 58.9%) with a female-to-male ratio of 1:0.7 (p < 0.001). The tongue (n = 1,196; 16.4%), lower lip (n = 1,005; 13.8%), and buccal mucosa (n = 997; 13.7%) were the most common anatomical sites. Reactive and inflammatory lesions (n = 3,840; 51.3%) were the most prevalent non-neoplastic pathologies (p < 0.001), followed by cysts (n = 475; 6.4%). Malignant neoplasms were more frequent (n = 1,353; 18.1%) than benign neoplasms (n = 512; 6.8%). Fibrous/fibroepithelial hyperplasia (n = 2,042; 53.2%) (p < 0.001) and squamous cell carcinoma (n = 1,191; 88.03%) (p < 0.001) were the most common oral lesions in older adults. Biopsy data allow the accurate characterization of the prevalence of oral and maxillofacial lesions, supporting the development of public health policies that can enable the prevention, early diagnosis, and appropriate treatment of these lesions. Also, they bring valuable information that helps dentists and geriatricians diagnose these diseases. literature age 6 years. . years) crosssectional cross sectional performed 20year year 20 period sex site color analyzed Pearsons Pearson s chisquare chi square groups 7476 7 476 7,47 n 4,487 4487 4 487 60.0%. 600 60.0% 0 60.0%) 4,403 4403 403 58.9% 589 58 9 femaletomale female male 10.7 107 1 0.7 1:0. p 0.001. 0001 0.001 001 1,196 1196 196 16.4%, 164 16.4% , 16 16.4%) 1,005 1005 005 13.8%, 138 13.8% 13 8 13.8%) 997 13.7% 137 sites 3,840 3840 3 840 51.3% 513 51 nonneoplastic non neoplastic 0.001, 475 6.4%. 64 6.4% 6.4%) 1,353 1353 353 18.1% 181 18 512 6.8%. 68 6.8% 6.8%) Fibrousfibroepithelial Fibrous fibroepithelial 2,042 2042 2 042 53.2% 532 53 1,191 1191 191 88.03% 8803 88 03 adults prevention Also 747 47 7,4 4,48 448 48 60.0 4,40 440 40 58.9 5 10 10. 07 0. 1:0 000 0.00 00 1,19 119 19 16.4 1,00 100 13.8 99 13.7 3,84 384 84 51.3 6.4 1,35 135 35 18.1 6.8 2,04 204 04 53.2 88.03 880 74 7, 4,4 44 60. 58. 1: 0.0 1,1 11 16. 1,0 13. 3,8 38 51. 6. 1,3 18. 2,0 53. 88.0 4, 1, 3, 2, 88.

https://doi.org/10.1590/1807-3107bor-2023.vol37.0098

Detección de Perkinsus marinus en una población silvestre del mejillón “choro” Modiolus capax del suroeste del Golfo de California
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Góngora-Gómez, Andrés Martín

; López-Meyer, Melina

; Villanueva-Fonseca, Lizeth Carolina

; Navarro-Chávez, María Fernanda

; Acosta-Campos, María José

; Hernández-Sepúlveda, Juan Antonio

; García-Ulloa, Manuel

Hidrobiológica

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dec 2021, Volume 31 N. 3 Pages 253 - 261

Abstract: En Es | Text: En Es | PDF: En | PDF: Es

ABSTRACT Background: After its transfaunation from the Atlantic Ocean has been reported, the protozoan Perkinsus marinus has expanded its list of hosts in several species of mollusks in the Pacific Ocean, both in cultivation and in wild populations. Goals: The presence of this parasite was evaluated in the horsemussel Modiolus capax, in the southwestern Gulf of California. Methods: 60 mussels were collected per annual season for one year (summer, autumn, winter 2019-spring 2020, N = 240), in the mining port of Santa Rosalía (SR), Baja California Sur, Mexico. Thioglycollate staining was used to detect presumptive hypnospores and the polymerase chain reaction (PCR) technique for its confirmation. The prevalence, parasite load and intensity of infection were obtained in each sampling. Results: Presumptive hypnospores of the parasite were detected with thioglycollate staining in almost 20% of the samples analyzed, of which only 5% were positive for confirmation with PCR. Neither the prevalence (6.66-33.33%) nor the parasite load (˂ 2.75 hypnospores/g) showed a tendency to increase with time; the intensity of infection ranged from negative to light. Salinity showed correlation with parasite load (r = 0.99, p = 0.002). Conclusions: The results suggest that M. capax in SR presented low susceptibility to the parasite, so its health is not compromised when hosting P. marinus. Although it is the first report of the protozoan in this mussel, continuous monitoring is recommended to know not only the health status of the bivalve in the region, but also of the other species of mollusks that inhabit there.

RESUMEN Antecedentes: Después de haberse reportado su transfaunación del océano Atlántico, el protozoario Perkinsus marinus ha ampliado su listado de hospederos en varias especies de moluscos del océano Pacífico, tanto en cultivo como de poblaciones silvestres. Objetivos: Se evaluó la presencia de este parásito en el mejillón “choro”, Modiolus capax, en el suroeste del Golfo de California. Métodos: Se colectaron 60 mejillones por estación anual durante un año (verano, otoño, invierno 2019-primavera 2020, n = 240), en el puerto minero de Santa Rosalía (SR), Baja California Sur, México. Se utilizó la tinción con tioglicolato para detectar presuntas hipnosporas y la técnica de reacción en cadena de la polimerasa (PCR) para su confirmación. Se obtuvieron la prevalencia, carga parasitaria e intensidad de infección en cada muestreo. Resultado: Se detectaron presuntas hipnosporas del parásito con la tinción de tioglicolato, en casi 20% de las muestras analizadas, de las que sólo el 5% fueron positivas para su confirmación con PCR. Ni la prevalencia (6.66-33.33%) ni la carga parasitaria (˂ 2.75 hipnosporas/g) mostraron una tendencia a aumentar con el tiempo; la intensidad de infección fue de negativa a ligera. La salinidad mostró correlación con la carga parasitaria (r = 0.99, p = 0.002). Conclusiones: Los resultados sugieren que M. capax en SR presentó baja susceptibilidad al parásito, por lo que su salud no se encuentra comprometida al hospedar a P. marinus. Aunque es el primer reporte del protozoario en este mejillón, es recomendable el monitoreo continuo para conocer, no solo el estado sanitario del bivalvo en la región, sino también, de las otras especies de moluscos que ahí habitan.

https://doi.org/10.24275/uam/izt/dcbs/hidro/2021v31n3/gongora

Building a model to analyse and compare social policies on ageing
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Bárrios, Maria João

; Fernandes, Ana Alexandre

; Fonseca, António Manuel

Forum Sociológico

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dec 2020, N. 37 Pages 5 - 18

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Resumo O envelhecimento populacional representa desafios para os indivíduos e para as organizações, no que respeita a questões económicas, sociais e de saúde. As políticas públicas procuram criar os recursos adequados e adaptar os dispositivos existentes, de forma que facilite e possibilite às pessoas um processo de envelhecimento com bem-estar. O paradigma de envelhecimento ativo, tal como é proposto pela OMS, tem grandes potencialidades na orientação das políticas dirigidas ao envelhecimento, em particular das intervenções locais. Nesta pesquisa propusemo-nos converter o conceito de envelhecimento ativo num instrumento de ordem prática, construído para ser aplicado por formuladores de políticas e investigadores, para avaliar e comparar diferentes políticas sociais relacionadas com o envelhecimento. Procedemos a uma validação de conteúdo e de construção do instrumento através da técnica Delphi. Através do instrumento criado, será possível analisar as políticas de acordo com a perspectiva de envelhecimento ativo, comparar programas e comunidades ou organizações, partilhar as boas práticas e identificar as ações políticas menos apropriadas, considerando-as prioridades de intervenção, adequando-as e melhorando-as.

Abstract Demographic ageing results in issues both for individuals and for organizations, with regards to social, economic and health issues. The challenge for public policies is to create the adequate resources and to adapt devices, in order to facilitate and allow an ageing process with well-being. The WHO’s proposal of Active Ageing has a great potential in guiding ageing policies, in particular local interventions. In this research we aimed to convert the active ageing concept into a practical instrument, built for use by policy-makers, researchers and stakeholders to evaluate and compare different social policies on ageing. We have proceeded to the validation of both the content and the design of the instrument, using the Delphi technique. Through the instrument created, it will be possible to analyse ageing policies from the active ageing point of view, to compare programmes and communities/organizations, to share the best policy practices and identify the least appropriate policy actions as the intervention priorities, adjusting and improving them.

https://doi.org/10.4000/sociologico.9347 27 downloads

Diretriz Brasileira de Cardio-oncologia – 2020
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Hajjar, Ludhmila Abrahão

; Costa, Isabela Bispo Santos da Silva da

; Lopes, Marcelo Antônio Cartaxo Queiroga

; Hoff, Paulo Marcelo Gehm

; Diz, Maria Del Pilar Estevez

; Fonseca, Silvia Moulin Ribeiro

; Bittar, Cristina Salvadori

; Rehder, Marília Harumi Higuchi dos Santos

; Rizk, Stephanie Itala

; Almeida, Dirceu Rodrigues

; Fernandes, Gustavo dos Santos

; Beck-da-Silva, Luís

; Campos, Carlos Augusto Homem de Magalhães

; Montera, Marcelo Westerlund

; Alves, Sílvia Marinho Martins

; Fukushima, Júlia Tizue

; Santos, Maria Verônica Câmara dos

; Negrão, Carlos Eduardo

; Silva, Thiago Liguori Feliciano da

; Ferreira, Silvia Moreira Ayub

; Malachias, Marcus Vinicius Bolivar

; Moreira, Maria da Consolação Vieira

; Valente Neto, Manuel Maria Ramos

; Fonseca, Veronica Cristina Quiroga

; Soeiro, Maria Carolina Feres de Almeida

; Alves, Juliana Barbosa Sobral

; Silva, Carolina Maria Pinto Domingues Carvalho

; Sbano, João

; Pavanello, Ricardo

; Pinto, Ibraim Masciarelli F.

; Simão, Antônio Felipe

; Dracoulakis, Marianna Deway Andrade

; Hoff, Ana Oliveira

; Assunção, Bruna Morhy Borges Leal

; Novis, Yana

; Testa, Laura

; Alencar Filho, Aristóteles Comte de

; Cruz, Cecília Beatriz Bittencourt Viana

; Pereira, Juliana

; Garcia, Diego Ribeiro

; Nomura, Cesar Higa

; Rochitte, Carlos Eduardo

; Macedo, Ariane Vieira Scarlatelli

; Marcatti, Patricia Tavares Felipe

; Mathias Junior, Wilson

; Wiermann, Evanius Garcia

; Val, Renata do

; Freitas, Helano

; Coutinho, Anelisa

; Mathias, Clarissa Maria de Cerqueira

; Vieira, Fernando Meton de Alencar Camara

; Sasse, André Deeke

; Rocha, Vanderson

; Ramires, José Antônio Franchini

; Kalil Filho, Roberto

Arquivos Brasileiros de Cardiologia

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nov 2020, Volume 115 N. 5 Pages 1006 - 1043

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

https://doi.org/10.36660/abc.20201006 4180 downloads

Atualização das Diretrizes Brasileiras de Valvopatias – 2020
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Tarasoutchi, Flavio

; Montera, Marcelo Westerlund

; Ramos, Auristela Isabel de Oliveira

; Sampaio, Roney Orismar

; Rosa, Vitor Emer Egypto

; Accorsi, Tarso Augusto Duenhas

; Santis, Antonio de

; Fernandes, João Ricardo Cordeiro

; Pires, Lucas José Tachotti

; Spina, Guilherme S.

; Vieira, Marcelo Luiz Campos

; Lavitola, Paulo de Lara

; Ávila, Walkiria Samuel

; Paixão, Milena Ribeiro

; Bignoto, Tiago

; Togna, Dorival Júlio Della

; Mesquita, Evandro Tinoco

; Esteves, William Antônio de Magalhães

; Atik, Fernando

; Colafranceschi, Alexandre Siciliano

; Moises, Valdir Ambrósio

; Kiyose, Alberto Takeshi

; Pomerantzeff, Pablo M. A.

; Lemos, Pedro A.

; Brito Junior, Fabio Sandoli de

; Weksler, Clara

; Brandão, Carlos Manuel de Almeida

; Poffo, Robinson

; Simões, Ricardo

; Rassi, Salvador

; Leães, Paulo Ernesto

; Mourilhe-Rocha, Ricardo

; Pena, José Luiz Barros

; Jatene, Fabio Biscegli

; Barbosa, Márcia de Melo

; Abizaid, Alexandre

; Ribeiro, Henrique Barbosa

; Bacal, Fernando

; Rochitte, Carlos Eduardo

; Fonseca, José Honório de Almeida Palma da

; Ghorayeb, Samira Kaissar Nasr

; Lopes, Marcelo Antonio Cartaxo Queiroga

; Spina, Salvador Vicente

; Pignatelli, Ricardo H.

; Saraiva, José Francisco Kerr

Arquivos Brasileiros de Cardiologia

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oct 2020, Volume 115 N. 4 Pages 720 - 775

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

https://doi.org/10.36660/abc.20201047 17824 downloads

Perkinsus marinus in the pleasure oyster Crassostrea corteziensis cultivated on the southeast coast of the Gulf of California, Mexico
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Villanueva-Fonseca, Lizeth Carolina

; García-Ulloa, Manuel

; López-Meyer, Melina

; Villanueva-Fonseca, Brenda Paulina

; Hernández-Sepúlveda, Juan Antonio

; Muñoz-Sevilla, Norma Patricia

; Góngora-Gómez, Andrés Martín

Latin american journal of aquatic research

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sep 2020, Volume 48 N. 4 Pages 529 - 537

Abstract: En | Text: En | PDF: En

ABSTRACT. The protozoan Perkinsus marinus has been associated with high mortality episodes of the eastern oyster Crassostrea virginica in the USA. The presence of P. marinus on the pleasure oyster Crassostrea corteziensis cultivated in two estuaries on the southeast coast of the Gulf of California was evaluated. Oysters were collected monthly (September 2016 to September 2017) and analyzed using Ray's fluid thioglycollate medium (RFTM) and polymerase chain reaction (PCR). Water parameters and oyster biometrics were also recorded. Pathogen prevalence increased over time from 0 to 100% in oysters from La Pitahaya, and from 0 to 83.33% in those from Bacorehuis. At both oyster farms, infection intensity was light (<1×104 parasites g-1 wet tissue), pathogen prevalence and infection intensity were correlated with oyster size and weight, and there was a strong correlation between P. marinus prevalence and intensity (La Pitahaya r = 0.91; Bacorehuis r = 0.82). The oysters that resulted positive for P. marinus by RFTM also were assayed using PCR. P. marinus presence was confirmed in 98.27% (114/116) of the oysters from La Pitahaya, and 95.83% (46/48) of those from Bacorehuis. The detection of P. marinus confirms that this pathogen is well established in the area with high expression during the warmer season. Despite the light infection intensity of this parasite at both sites, health surveillance of this bivalve in the region is highly advisable.

https://doi.org/10.3856/vol48-issue4-fulltext-2463 315 downloads

Desarrollo y validación de un método de cromatografía gaseosa para la determinación de mentol, salicilato de metilo, timol y ácido benzoico en solución antiséptica
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Fonseca Gola, Antonio

; Cordoví Velázquez, Juan Manuel

; Benítez Guerra, Niurka

; Fernández Cervera, Mirna

Revista Colombiana de Ciencias Químico - Farmacéuticas

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aug 2020, Volume 49 N. 2 Pages 306 - 328

Abstract: Es En | Text: Es En | PDF: Es | PDF: En

RESUMEN Se desarrolló y validó un método por cromatografía gaseosa para la cuantificación simultánea de mentol (MT), salicilato de metilo (SM), timol (TM) y ácido benzoico (AB) en una solución antiséptica bucal. Se utilizó una columna DB-WAX y dietilenglicol como estándar interno. Se determinó la linealidad en un intervalo de concentraciones de 64,00 a 96,00 µg/mL (MT), 80,00 a 120,00 µg/mL (SM), 96,00 a 144,00 µg/mL (TM) y 48,00 a 72,00 µg/mL (AB), obteniendo coeficientes de correlación superiores a 0,999, y coeficientes de variación de los factores de respuestas de 1,18, 1,95, 3,52 y 1,48%, respectivamente. Se establecieron límites de detección de 0,51; 1,14; 3,34 y 1,402 ng/mL para el MT, SM, TM y AB, respectivamente, mientras los límites de cuantificación fueron de 1,45, 3, 43, 9, 73 y 4, 36 ng/mL en cada caso. Los porcentajes de recuperación fueron de 100,03, 99, 31, 99, 92 y 100,12; con coeficientes de variación de 0,42, 0,79, 0,66 y 0,76% para cada caso. El método fue lineal, exacto, preciso y selectivo para la determinación de los analitos en el control de calidad.

SUMMARY A method was developed and validated by gas chromatography for the simultaneous quantification of menthol (MT), methyl salicylate (SM), thymol (TM) and benzoic acid (AB) in an oral antiseptic solution. A DB-WAX column and diethylene glycol was used as internal standard. Linearity was determined in a concentration range of 64.00 to 96.00 µg/mL (MT), 80.00 to 120.00 µg/mL (SM), 96.00 to 144.00 µg/mL (TM) and 48.00 to 72.00 µg/mL (AB) achieving correlation coefficients greater than 0.999, and coefficients of variation of the response factors of 1.18, 1.95, 3.52 and 1.48%, respectively. Detection limits were established: 0.51, 1.14, 3.34 and 1.402 ng/mL for MT, SM, TM, and AB, respectively, while the quantification limits were 1.45, 3.43, 9.73 and 4.36 ng/mL in each case. Recovery percentages were 100.03, 99.31, 99.92 and 100.12; with coefficients of variation of 0.42, 0.79, 0.66 and 0.76% for each case. The method was linear, accurate, precise, and selective for the determination of analytes in quality control.

https://doi.org/10.15446/rcciquifa.v49n2.89484 785 downloads

10.

TRACE METALS IN TWO WILD POPULATIONS OF THE SQUALID CALLISTA CLAM ( Megapitaria squalida) IN THE SOUTHEASTERN GULF OF CALIFORNIA, MEXICO
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Sepúlveda, Carlos Humberto

; Góngora-Gómez, Andrés Martín

; Pérez-Álvarez, Sandra

; Rodríguez-González, Hervey

; Muñoz-Sevilla, Norma Patricia

; Villanueva-Fonseca, Brenda Paulina

; Hernández-Sepúlveda, Juan Antonio

; García-Ulloa Gómez, Manuel

Revista internacional de contaminación ambiental

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2020, Volume 36 N. 3 Pages 667 - 676

Abstract: En Es | Text: En Es | PDF: En | PDF: Es

ABSTRACT The squalid callista clam (Megapitaria squalida) is a popular raw seafood item for human consumption; however, as a filter feeder, this clam accumulates heavy metals from natural and anthropogenic sources. The concentrations of copper (Cu), cadmium (Cd), chromium (Cr), nickel (Ni), lead (Pb), zinc (Zn), arsenic (As), and mercury (Hg) in sediments and the soft tissues of M. squalida from two sites on the southeastern coast of the Gulf of California, Mexico, were evaluated from April 2016 to April 2017 on a monthly basis. The metal concentrations in sediments from both sites did not exceed the Mexican and international regulations. Concentrations of Cd and Pb in the clams from Altata bay (2.49 and 5.68 µg/g dw, respectively) and Agiabampo bay (2.38 and 5.54 µg/g dw, respectively) exceeded the permissible limits recommended by Mexican sanitary regulations, thus representing a threat to human health. The higher values of Cd, As, and Hg obtained for the biota sediment accumulation factor in both sampling areas indicate that squalid callista is a strong accumulator of these metals. The metal burdens in sediments and M. squalida soft tissues are influenced by chemicals from agriculture and aquaculture, as well as urban sewage disposal near both sites. This study brings useful information on metal bioaccumulation in one of the most important commercial clam species on the Pacific coast.

RESUMEN La almeja callista escuálida (Megapitaria squalida) es un marisco popular que se consume crudo, pero por ser filtrador, puede acumular metales pesados de fuentes naturales y antrópicas. En el presente trabajo se evaluaron, de abril de 2016 a abril de 2017, las concentraciones mensuales de cobre (Cu), cadmio (Cd), cromo (Cr), níquel (Ni), plomo (Pb), zinc (Zn), arsénico (As) y mercurio (Hg) en sedimentos y tejidos blandos de M. squalida de dos sitios de la costa sureste del Golfo de California, México. Los niveles de metales en los sedimentos de ambos sitios no excedieron las regulaciones mexicanas e internacionales. Las concentraciones de Cd y Pb en las almejas de la bahía de Altata (2.49 y 5.68 µg/g ps, respectivamente) y la bahía de Agiabampo (2.38 y 5.54 µg/g ps, respectivamente) sí excedieron los límites permisibles recomendados por las regulaciones sanitarias mexicanas, lo que representa una amenaza para la salud humana. El factor de acumulación de sedimentos en la biota para las dos áreas muestreadas indica que la almeja callista escuálida es un acumulador fuerte de Cd, As y Hg. Las concentraciones metálicas en los sedimentos y los tejidos blandos de M. squalida están influenciadas por los productos químicos utilizados en la agricultura y la acuicultura, así como por el suministro de aguas residuales urbanas cercanas a ambos sitios. Este estudio brinda información útil sobre la bioacumulación de metales en una de las especies de almejas comerciales más importantes de la costa del Pacífico.

https://doi.org/10.20937/rica.53565 204 downloads

11.

Prevalencia de Perkinsus sp. (Apicomplexa) en la almeja chocolata Megapitaria squalida de la costa central de Sinaloa, México
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Góngora-Gómez, Andrés Martín

; Sandoval-Rivera, Pedro Antonio

; Villanueva-Fonseca, Lizeth Carolina

; López-Meyer, Melina

; Sainz-Hernández, Juan Carlos

; Espinosa-Alonso, Laura Gabriela

; Escobedo-Urías, Diana Cecilia

; Hernández-Sepúlveda, Juan Antonio

; Rodríguez-González, Hervey

; García-Ulloa, Manuel

Hidrobiológica

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dec 2019, Volume 29 N. 3 Pages 129 - 136

Abstract: En Es | Text: En Es | PDF: En | PDF: Es

ABSTRACT Background: Different species of Perkinsus spp. have been associated to mortality of commercial mollusks. Goals: The prevalence of Perkinsus sp. in the callista clam Megapitaria squalida was evaluated monthly (June 2015 to November 2016) to determine the relationship between the infection and size of clam. Methods: 5,000 clam seeds (5.73 mm height of the shell and 0.03 g of initial weight) were placed in a fishing refuge of Altata, Sinaloa. A total of 60 specimens were collected each month to evaluate their growth and parasite prevalence. The water physicochemical parameters (temperature, salinity, pH, dissolved oxygen, depth, and transparency) and the length, height, and width of the shell and final weight of M. squalida were obtained. Results: Ray’s thioglycollate fluid medium test (MFTR) detected spherical cells (10X) of dark color indicating the presence of presumed hypnospores of Perkinsus sp. during the first 17 months of sampling. The mean infection intensity was grade 2 (mild), while the maximum prevalence was 43.33% in the months of November (2015) and March (2016), with an average of 21.05%. None of the Perkinsus sp sample (n = 112) positively detected by MFTR confirmed the presence of the protozoan. There was no correlation (p >0.05) between the prevalence of the pathogen with all the parameters studied. Conclusions: The results suggest that M. squalida is not susceptible to Perkinsus sp. The present represents the first report of Perkinsus sp. in the callista clam M. squalida.

RESUMEN Antecedentes: Diferentes especies del protozoario Perkinsus spp. han sido asociados con mortalidades de moluscos comerciales. Objetivos: Se evaluó la prevalencia de Perkinsus sp. en la almeja Megapitaria squalida (junio 2015 a noviembre de 2016) para determinar la relación de la infección con la talla. Métodos: Se sembraron 5,000 semillas (5.73 mm altura de la concha y 0.03 g de peso inicial) en un refugio pesquero de Altata, Sinaloa. Se recolectaron 60 ejemplares cada mes para evaluar su crecimiento y prevalencia del parásito. Se obtuvieron los parámetros fisicoquímicos del agua (temperatura, salinidad, pH, oxígeno disuelto, profundidad y transparencia) y la longitud, largo y ancho de la concha y peso final de M. squalida. Resultados: La prueba de medio fluido de tioglicolato de Ray (MFTR) detectó células esféricas (10X) de color obscuro, indicando la presencia de presuntas hipnosporas de Perkinsus sp. durante los primeros 17 meses de muestreo. La intensidad de la infección promedio fue de grado 2 (leve), mientras que la prevalencia máxima fue de 43.33% en los meses de noviembre (2015) y marzo (2016), con un promedio de 21.05%. Ninguna de las muestras positivas (n = 112) de Perkinsus sp. con MFTR confirmó la presencia del protozoario. No se obtuvo correlación (p >0.05) entre la prevalencia del patógeno con todos los parámetros estudiados. Conclusiones: Los resultados sugieren que M. squalida es poco susceptible a la infección de Perkinsus sp. El presente trabajo representa el primer reporte de Perkinsus sp. en la almeja chocolata M. squalida.

https://doi.org/10.24275/uam/izt/dcbs/hidro/2020v29n3/gongora 105 downloads

12.

External Iliac Artery Laceration Caused by Hip Prosthesis Migration
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Moura, Diogo Lino

; Moreira, Mário

; Antunes, Luís

; Agostinho, Alfredo Gil

; Fonseca, Manuel

; Albuquerque, António

Revista Brasileira de Ortopedia

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oct 2019, Volume 54 N. 5 Pages 597 - 600

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Resumo A artroplastia da anca é uma intervenção frequente e segura na cirurgia ortopédica. No entanto, a proximidade dessa articulação com vasos de grande calibre faz com que a ocorrência de lesão vascular maior seja uma complicação rara, mas grave e possivelmente letal, dessa técnica cirúrgica. As lesões vasculares agudas no contexto de uma artroplastia da anca têm etiologia e apresentação clínica variáveis e são mais frequentes em cirurgias de revisão e situações de migração medial intrapélvica e de infecção crônica de próteses da anca. No presente artigo, os autores apresentam um caso com complicação vascular maior aguda e tardia em contexto de revisão de prótese da anca. Trata-se de um paciente que desenvolveu uma laceração aguda da artéria ilíaca externa em contexto de migração acetabular intrapélvica crônica progressiva da prótese da anca associada a infecção crônica.

Abstract Hip arthroplasty is a common and safe intervention in orthopedic surgery. However, the proximity of this joint to large vessels makes the occurrence of vascular injury a rare but serious and possibly lethal complication of this surgical technique. Acute vascular injuries in the context of a hip arthroplasty have variable etiologies and clinical presentations, and are more common in revision surgeries and in situations of medial intrapelvic migration and of chronic infection of the hip prosthesis. In the present article, the authors present a case of acute and late major vascular complication in the context of hip arthroplasty revision. The patient developed an acute laceration of the external iliac artery caused by chronic and progressive medial intrapelvic acetabular migration of the hip prosthesis associated with chronic infection.

https://doi.org/10.1016/j.rbo.2017.09.020 454 downloads

13.

Updated Cardiovascular Prevention Guideline of the Brazilian Society of Cardiology - 2019
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Précoma, Dalton Bertolim

; Oliveira, Gláucia Maria Moraes de

; Simão, Antonio Felipe

; Dutra, Oscar Pereira

; Coelho, Otávio Rizzi

; Izar, Maria Cristina de Oliveira

; Póvoa, Rui Manuel dos Santos

; Giuliano, Isabela de Carlos Back

; Alencar Filho, Aristóteles Comte de

; Machado, Carlos Alberto

; Scherr, Carlos

; Fonseca, Francisco Antonio Helfenstein

; Santos Filho, Raul Dias dos

; Carvalho, Tales de

; Avezum Jr., Álvaro

; Esporcatte, Roberto

; Nascimento, Bruno Ramos

; Brasil, David de Pádua

; Soares, Gabriel Porto

; Villela, Paolo Blanco

; Ferreira, Roberto Muniz

; Martins, Wolney de Andrade

; Sposito, Andrei C.

; Halpern, Bruno

; Saraiva, José Francisco Kerr

; Carvalho, Luiz Sergio Fernandes

; Tambascia, Marcos Antônio

; Coelho-Filho, Otávio Rizzi

; Bertolami, Adriana

; Correa Filho, Harry

; Xavier, Hermes Toros

; Faria-Neto, José Rocha

; Bertolami, Marcelo Chiara

; Giraldez, Viviane Zorzanelli Rocha

; Brandão, Andrea Araújo

; Feitosa, Audes Diógenes de Magalhães

; Amodeo, Celso

; Souza, Dilma do Socorro Moraes de

; Barbosa, Eduardo Costa Duarte

; Malachias, Marcus Vinícius Bolívar

; Souza, Weimar Kunz Sebba Barroso de

; Costa, Fernando Augusto Alves da

; Rivera, Ivan Romero

; Pellanda, Lucia Campos

; Silva, Maria Alayde Mendonça da

; Achutti, Aloyzio Cechella

; Langowiski, André Ribeiro

; Lantieri, Carla Janice Baister

; Scholz, Jaqueline Ribeiro

; Ismael, Silvia Maria Cury

; Ayoub, José Carlos Aidar

; Scala, Luiz César Nazário

; Neves, Mario Fritsch

; Jardim, Paulo Cesar Brandão Veiga

; Fuchs, Sandra Cristina Pereira Costa

; Jardim, Thiago de Souza Veiga

; Moriguchi, Emilio Hideyuki

; Schneider, Jamil Cherem

; Assad, Marcelo Heitor Vieira

; Kaiser, Sergio Emanuel

; Lottenberg, Ana Maria

; Magnoni, Carlos Daniel

; Miname, Marcio Hiroshi

; Lara, Roberta Soares

; Herdy, Artur Haddad

; Araújo, Cláudio Gil Soares de

; Milani, Mauricio

; Silva, Miguel Morita Fernandes da

; Stein, Ricardo

; Lucchese, Fernando Antonio

; Nobre, Fernando

; Griz, Hermilo Borba

; Magalhães, Lucélia Batista Neves Cunha

; Borba, Mario Henrique Elesbão de

; Pontes, Mauro Ricardo Nunes

; Mourilhe-Rocha, Ricardo

Arquivos Brasileiros de Cardiologia

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oct 2019, Volume 113 N. 4 Pages 787 - 891

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

https://doi.org/10.5935/abc.20190204 40841 downloads

14.

GLA Gene Mutation in Hypertrophic Cardiomyopathy with a New Variant Description: Is it Fabry's Disease?
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Chaves-Markman, Ândrea Virgínia

; Markman, Manuel

; Calado, Eveline Barros

; Pires, Ricardo Flores

; Santos-Veloso, Marcelo Antônio Oliveira

; Pereira, Catarina Maria Fonseca

; Lordsleem, Andréa Bezerra de Melo da Silveira

; Lima, Sandro Gonçalves de

; Markman Filho, Brivaldo

; Oliveira, Dinaldo Cavalcanti de

Arquivos Brasileiros de Cardiologia

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jul 2019, Volume 113 N. 1 Pages 77 - 84

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Resumo Fundamento: A doença de Fabry (DF) é uma doença de armazenamento lisossômico ligada ao cromossomo X, devido a mutações no gene da alfa galactosidase A (GLA), levando a deficiência enzimática de alfa-galactosidase (α-Gal A) e acúmulo de globotriaosilceramida (Gb3) e globotriaosilsulfingosina (liso-Gb3), causando disfunção de múltiplos órgãos. Objetivo: realizar a triagem do gene GLA em um grupo de pacientes com diagnóstico ecocardiográfico de cardiomiopatia hipertrófica (CMH). Métodos: estudo transversal realizado com pacientes com CMH em um hospital universitário. Pacientes com doença arterial coronariana e valvopatias foram excluídos. Foi realizada análise de mutação do gene GLA. Em indivíduos do sexo masculino, a análise foi realizada após evidência de baixa atividade de α-Gal A. Resultados: Foram incluídos 60 pacientes com diagnostico ecocardiográfico de CMH. A idade variou de 12 a 85 anos e 60% eram mulheres. O percentual médio de fibrose miocárdica na RM foi 10,7 ± 13,1% e a espessura ventricular média foi 18,7 ± 6,7 mm. Quatro pacientes tinham as seguintes mutações do GLA: c.967C>A (p.Pro323Thr), ainda não descrita na literatura; c.937G>T (p.Asp313Tyr); e c.352C>T (p.Arg118Cys). Todos os pacientes apresentavam níveis normais de liso-Gb3 e fibrose miocárdica não isquêmica na ressonância magnética; um paciente apresentou proteinúria; um paciente apresentou taquicardia ventricular. Conclusão: Neste estudo, a frequência de mutação no gene GLA em pacientes com CMH foi 6,7%. Uma nova mutação no exon 6 do gene GLA, c.967C>A (p.Pro323Thr), foi identificada. Pacientes com CMH podem ter mutações do GLA e a DF deve ser excluída. Os níveis plasmáticos de (liso-Gb3) não parecem ser suficientes para fazer um diagnóstico e biópsia de órgãos deve ser considerada.

Abstract Background: Fabry disease (FD) is an X-linked lysosomal storage disorder caused by mutations in the alpha galactosidase A gene (GLA) that lead to the enzymatic deficiency of alpha galactosidase (α-Gal A), resulting in the accumulation of globotriaosylceramide (Gb3) and globotriaosylsphingosine (lyso-Gb3), causing multiple organ dysfunctions. Objective: To perform GLA gene screening in a group of patients with echocardiographic diagnosis of hypertrophic cardiomyopathy (HCM). Methods: a cross-sectional study was conducted with HCM patients from a university hospital. Patients with coronary artery disease and valvulopathies were excluded. Mutation analysis of the GLA gene was performed. In male subjects, the analysis was performed after evidence of low α-Gal A activity. Results: 60 patients with echocardiographic diagnosis of HCM were included. Age ranged from 12 to 85 years and 60% were women. Mean myocardial fibrosis percentage on MRI was 10.7 ± 13.1% and mean ventricular thickness was18.7 ± 6.7 mm. Four patients had the following GLA gene mutations: c.967C>A (p.Pro323Thr), not yet described in the literature; c.937G>T (p.Asp313Tyr); and c.352C>T (p.Arg118Cys). All patients had normal levels of lyso-Gb3 and non-ischemic myocardial fibrosis on magnetic resonance imaging; one patient had proteinuria and one patient had ventricular tachycardia. Conclusion: in this study, the frequency of mutation in the GLA gene in patients with HCM was 6.7%. A novel mutation in exon 6 of the GLA gene, c.967C>A (p.Pro323Thr), was identified. Patients with HCM may have GLA mutations and FD should be ruled out. Plasma (lyso-Gb3) levels do not seem to be sufficient to attain a diagnosis and organ biopsy should be considered.

https://doi.org/10.5935/abc.20190112 1265 downloads

15.

The Expression of and Preoperative Correlation between Heat-Shock Protein 70, EuroSCORE, and Lactate in Patients undergoing CABG with Cardiopulmonary Bypass
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Cantero, Marcos Antonio

; Almeida, Rui Manuel Siqueira

; Morato, Priscila Neder

; Santos-Junior, Valfredo de Almeida

; Moura, Carolina Soares

; Amaya-Farfan, Jaime

; Fonseca, João Luis

; Lollo, Pablo Christiano Barboza

Brazilian Journal of Cardiovascular Surgery

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apr 2019, Volume 34 N. 2 Pages 156 - 164

Abstract: En | Text: En | PDF: En

Abstract Objetive: Coronary artery bypass grafting (CABG) with cardiopulmonary bypass (CPB) improved symptoms and increased survival and quality of life in patients with coronary artery disease. However, it should be the main cause of a complex organic systemic inflammatory response that greatly contributes to several postoperative adverse effects. Methods: We aimed to evaluate heat-shock protein 70 (HSP 70) expression as a morbimortality predictor in patients with preserved ventricular function undergoing coronary artery bypass grafting (CABG) with cardiopulmonary bypass (CPB) and to determine their association with the lactate as a marker of tissue hypoperfusion and the EuroSCORE risk score. This is a prospective, observational study including 46 patients and occurring between May and July 2016. Patients without ventricular dysfunction undergoing myocardial revascularization with extracorporeal circulation were included. They were divided into (1) complicated and (2) uncomplicated postoperative evolution groups. EuroSCORE, lactate levels, and HSP 70 expression and their correlations were determined. Results: Statistical analysis showed that the group with complicated evolution had higher EuroSCORE values than the other group. HSP 70 protein levels were significantly increased in the group with uncomplicated evolution and showed similar results. According to our results, HSP family proteins may be independent predictors of uncomplicated evolution in patients without ventricular dysfunction undergoing CABG with CPB. Conclusion: HSP 70 should be a good discriminator and protection marker for complications in cardiac surgery.

https://doi.org/10.21470/1678-9741-2018-0231 640 downloads

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