Leafcutter ants are one of the most important crop pests in the Neotropics. In the present study, several specimens were collected from four provinces in Argentina in order to determine the species of phorid fies parasitizing them. We report our findings on seven phorid species, Apocephalus neivai Borgmeier, Ap. noetingerorum Disney, Neodohrniphora unichaeta Disney, Myrmosicarius catharinensis Borgmeier, M. cristobalensis Disney, M. crudelis Borgmeier and M. gracilipes Borgmeier, over the leafcutter ants Acromyrmex ambiguus Emery, Ac. crassispinus Forel, Ac. heyeri Forel, Ac. lobicornis Emery and Ac. lundii Guérin. All the known hosts and geographic ranges for these seven phorids in Argentina are provided. The seven phorid species represent new records for all the provinces studied, expanding their geographical ranges; furthermore, we establish a total of eleven new host-parasitoid interactions. Our data expand the knowledge of this little studied system and provide important considerations for the potential use of these parasitoids as biological control candidates of the leafcutter ants.

Las hormigas cortadoras de hojas son una de las plagas más importantes de la región Neotropical. En el presente estudio, fueron recolectados representantes de cuatro provincias de Argentina, con el objetivo de identifcar las especies de fóridos que las parasitan. Como resultado, se registraron siete especies de fóridos, Apocephalus neivai Borgmeier, Ap. noetingerorum Disney, Neodohrniphora unichaeta Disney, Myrmosicarius catharinensis Borgmeier, M. cristobalensis Disney, M. crudelis Borgmeier y M. gracilipes Borgmeier, obtenidos en hormigas cortadoras de hojas de las especies Acromyrmex ambiguus Emery, Ac. crassispinus Forel, Ac. heyeri Forel, Ac. lobicornis Emery y Ac. lundii Guérin. Asimismo, son reportados todos los registros previos de hospedadores y su distribución geográfca en Argentina para las siete especies de fóridos. Se citan nuevos registros en todas las especies estudiadas, ampliando así sus rangos geográfcos y estableciendo once nuevas interacciones hospedador-parasitoide. Nuestros datos amplían el conocimiento de este poco estudiado sistema y proveen importantes consideraciones para el uso potencial de estos parasitoides como controladores biológicos de hormigas cortadoras de hojas.

Pseudacteon flies, parasitoids of worker ants, are being intensively studied as potentially effective agents in the biological control of the invasive pest fire ant genus Solenopsis (Hymenoptera: Formicidae). This is the first attempt to describe the karyotype of P. curvatus Borgmeier, P. nocens Borgmeier and P. tricuspis Borgmeier. The three species possess 2n = 6; chromosomes I and II were metacentric in the three species, but chromosome pair III was subtelocentric in P. curvatus and P. tricuspis, and telocentric in P. nocens. All three species possess a C positive band in chromosome II, lack C positive heterochromatin on chromosome I, and are mostly differentiated with respect to chromosome III. P. curvatus and P. tricuspis possess a C positive band, but at different locations, whereas this band is absent in P. nocens. Heterochromatic bands are neither AT nor GC rich as revealed by fluorescent banding. In situ hybridization with an 18S rDNA probe revealed a signal on chromosome II in a similar location to the C positive band in the three species. The apparent lack of morphologically distinct sex chromosomes is consistent with proposals of environmental sex determination in the genus. Small differences detected in chromosome length and morphology suggests that chromosomes have been highly conserved during the evolutionary radiation of Pseudacteon. Possible mechanisms of karyotype evolution in the three species are suggested.

ABSTRACT. Pseudacteon Coquillett phorid flies oviposit on Solenopsis Westwood ants and pupate within the ant's head. We have evaluated the relationship between pupae's viability, presence of respiratory horns and the operculum color in four species of Pseudacteon reared on Solenopsis invicta Buren and Solenopsis richteri Forel ants. The presence of respiratory horns was significantly associated with pupae's viability for all species considered (p < 0,001) but operculum color was not (p > 0,09), except (p < 0,01) when Pseudacteon cultellatus Borgmeier had developed on S. invicta nanitic and reserve workers. Pupae with light-colored opercula were more frequent in P. cultellatus, whereas brown opercula were more frequent for the other species that attack bigger ants. Mimetism can be invoked to explain the similarity in opercula color with that of the head of the parasitized ant as a way to avoid recognition by members of the colony. We conclude that the presence of respiratory horns is necessary for pupae survival of most of the pupae and we suggest to use the presence of respiratory horns as an indicator of the efficiency of rearing protocols for this group of parasitoids. We also recommend using forager ants because other casts do not seem to be appropriate hosts.

RESUMEN. Fóridos del género Pseudacteon Coquillett oviponen en forrajeras del género Solenopsis Westwood y empupan en sus cabezas. Se evaluó la relación entre la viabilidad de los parasitoides, la presencia de cuernos respiratorios y el color en los opérculos de los puparios de cuatro especies de Pseudacteon criados sobre Solenopsis invicta Buren y Solenopsis richteri Forel. La presencia de cuernos respiratorios estuvo asociada a la viabilidad de las pupas para las especies consideradas (p < 0,01), pero no el color de los opérculos (p > 0,09); excepto cuando Pseudacteon cultellatus Borgmeier se desarrolló sobre hormigas de la reserva y niñeras de S. invicta (p < 0,01). Los opérculos claros predominaron en P. cultellatus; mientras que en las demás especies, que atacan a hormigas más grandes, predominaron los opérculos castaños. Debido a que los opérculos presentaron un color similar al de la hormiga parasitada, esto representaría un mimetismo por parte del parasitoide para evitar ser detectado por el huésped. Dado que los cuernos respiratorios son necesarios para la supervivencia de la mayoría de las pupas, sugerimos que sean usados como indicadores de eficiencia en los protocolos de cría. Recomendamos usar solo forrajeras dado que las otras castas no parecen ser huéspedes apropiados.