Abstract Understanding forest structure and diversity allows the development and analysis of strategies for the sustainable management of natural resources. The objective of the research was to contribute to the knowledge of tree structure, as well as its regeneration capacity in an altitudinal gradient in a Natural Protected Area in Madera, Chihuahua. The study included three altitudinal intervals: lower (2 307 to 2 466 m), middle (2 466 to 2 625 m) and upper (2 625 - 2 785 m), in which 30 circular sites of 500 m2 were distributed where the diameter and height of the upper trees were measured, as well as the density of regeneration. The vertical structure was obtained with the Pretzsch index (A), in addition, the importance value index (IVI), diversity with the Shannon-Wiener index (H') and richness with Margalef (D mg ) were evaluated, as well as the renewal capacity and damage present. The values of A show the greatest structural homogeneity in interval 2; the Pinaceae family registered the highest ecological weight in the IVI; the values of H' and Dmg were higher in stratum II, however, all are considered of low diversity and richness; the species Abies concolor and Pseudotsuga menziesii, showed the highest proportion of regeneration in the community, in which suppression is the frequent damage, however, 95% of the individuals were not affected. This information is useful for the conservation of these ecologically important communities in temperate forests.

Resumen Comprender la estructura y diversidad forestal permite elaborar y analizar estrategias para el manejo sustentable de los recursos naturales. El objetivo de la investigación fue contribuir al conocimiento de la estructura arbórea, así como su capacidad de regeneración en un gradiente altitudinal en un Área Natural Protegida, en Madera, Chihuahua. El estudio comprendió tres intervalos altitudinales: inferior (2 307 a 2 466 m), medio (2 466 a 2 625 m) y superior (2 625 - 2 785 m), en los que se distribuyeron 30 sitios circulares de 500 m2 donde se midió el diámetro y altura del arbolado superior, así como la densidad de la regeneración. La estructura vertical se obtuvo con el índice de Pretzsch (A), además, se evaluó el índice de valor de importancia (IVI), la diversidad con el índice de Shannon-Wiener (H’) y riqueza con Margalef (D mg ), asimismo, se estimó la capacidad de renuevo y los daños presentes. Los valores de A muestran la mayor homogeneidad estructural en el intervalo 2; la familia Pinaceae registró el peso ecológico superior en el IVI; los valores de H’ y D mg fueron superiores en el estrato II, sin embargo, todos se consideran de baja diversidad y riqueza; las especies Abies concolor y Pseudotsuga menziesii, mostraron la mayor proporción de regeneración en la comunidad, en la que la supresión es el daño frecuente, no obstante, el 95% de los individuos no presentaron afectaciones. Esta información es de utilidad para la conservación de estas comunidades de importancia ecológica en bosques templados.

Resumo Este estudo exploratório apresenta os principais resultados da análise do rastreio (tracking) do uso de aplicações móveis por 342 jovens adultos/as (18-30 anos) em Portugal, considerando as relações de poder com base no género que podem ser refletidas nas suas utilizações. Os resultados indicam que as apps de Redes Sociais e Mensageiros são predominantes, sendo que as categorias de auto-rastreio (Saúde e Bem-estar, Fitness e Self-tracking) são as de menor uso (tendo maior peso em classes socioeconómicas favorecidas). Ainda que existam mais mulheres do que homens a utilizar essas apps, mantendo-se as estruturas genderizadas, estas plataformas digitais também poderão ser concebidas como espaços para o crescimento do auto-rastreio e do autocuidado masculino.

Abstract This exploratory study presents the main results from the analysis of the tracking of mobile application use by 342 young adults (18-30 years old) in Portugal considering the gendered power relations that may be reflected in those uses. The results indicate that Social Media and Messaging apps are predominant, while the tracking of the self categories (Health and Well-being, Fitness and Self-tracking) are the least used (with greater weight in privileged socioeconomic classes). Although there are more women than men using these apps, thus preserving gendered structures, these digital platforms may also be conceived as venues for the growth of male self-tracking and self-care.

Resumen Este estudio exploratorio presenta los principales resultados del análisis del rastreo del uso de aplicaciones móviles por 342 adultos jóvenes (18-30 años) en Portugal, considerando las relaciones de poder de género que pueden ser reflejadas en estos usos. Los resultados indican que las apps de Redes Sociales y Mensajes predominan, mientras que las categorías de auto-rastreo (Salud y Bienestar, Fitness y Self-tracking) son las menos utilizadas (teniendo mayor peso en las clases socioeconómicas favorecidas). Aunque el número de mujeres usuarias de estas apps es mayor que el de hombres, manteniéndose las estructuras de género, estas plataformas digitales también pueden concebirse como espacios para el crecimiento del auto-rastreo y autocuidado masculino.

ABSTRACT Slope exposure is one of the environmental factors that influences vegetation characteristics. Although there are studies on this aspect in various types of vegetation, up to now submontane shrublands have not been extensively studied. In this work, the effect of slope exposure on the structure and composition of plant species in a state park in northeastern Mexico where the dominant vegetation is submontane scrub was evaluated. Ten 10 x 10 m exposure sites were sampled: northeast, southwest and northwest. Total height (h), basal diameter (d 0.10) and crown diameter (k) were measured for each individual. Our hypotheses were: 1) the northwestern exposure community will have greater basal area, canopy cover, volume, absolute abundance, and specific richness due to higher humidity, 2) each slope will present dissimilarity in species composition. As results, nine families, 17 genera and 17 species of vascular plants were recorded. Fabaceae was the best represented family with six species. The species with the highest importance value index were the endemic shrub Neopringlea integrifolia in the northeast exposure (21.52 %) and the exotic tree Leucaena leucocephala in the southwest (16.17 %) and northwest (27.16 %) exposures. It is concluded that the plant community of the northwest exposure has a greater basal area, crown cover, volume, absolute abundance and specific richness. However, there was no real difference in the species composition of the three exposures.

RESUMEN La exposición de ladera es uno de los factores ambientales que influye sobre características de la vegetación. Aunque existen trabajos sobre este aspecto en diversos tipos de vegetación, hasta ahora los matorrales submontanos no han sido muy estudiados. En este trabajo se evaluó el efecto de la exposición de las laderas en la estructura y composición de especies de plantas en un parque estatal del noreste de México donde la vegetación dominante es matorral submontano. Se muestrearon 10 sitios de 10 x 10 m en exposición: noreste, suroeste y noroeste. A cada individuo se le midió la altura total (h), diámetro basal (d 0,10) y diámetro de copa (k). Nuestras hipótesis fueron: 1) la comunidad de la exposición noroeste tendrá mayor área basal, cobertura de dosel, volumen, abundancia absoluta y riqueza específica debido a la mayor humedad, 2) cada ladera presentará disimilitud en la composición de especies. Como resultados se registraron nueve familias, 17 géneros y 17 especies de plantas vasculares. Fabaceae fue la familia mejor representada con seis especies. Las especies con mayor índice de valor de importancia fueron la arbustiva endémica Neopringlea integrifolia en la exposición noreste (21,52 %) y la exótica arbórea Leucaena leucocephala en las exposiciones suroeste (16,17 %) y noroeste (27,16 %). Se concluye que la comunidad vegetal de la exposición noroeste tiene mayor área basal, cobertura de copa, volumen, abundancia absoluta y riqueza específica. Sin embargo, no hubo diferencia real en la composición de especies de las tres exposiciones.

Abstract Social network analysis is a method that allows the identification and examination of structures of various types of data, objects, or user groups, as well as the interactions created by a community and the relationships that exist between them. To characterize the virtual community of a higher education institution, the Fruchterman-Reingold, Yifan Hu, and Noverlap algorithms were applied using the Gephi tool for the analysis and modeling of 30 Facebook fan pages dedicated to the promotion of culture and the dissemination of science. The results show a relationship with the direction and number of edges for each of the 30 nodes for each algorithm applied. The focus of the analysis provided information about the dynamics of the virtual university community, allowing a visual understanding of how members connect and communicate within the Facebook social network.

Resumen El análisis de red social es un método que permite identificar y examinar estructuras de diversos tipos de datos, objetos o grupos de usuarios, así como las interacciones creadas por una comunidad y las relaciones que existen entre ellas. Con el objetivo de caracterizar la comunidad virtual de una institución de educación superior, se aplicaron los algoritmos Fruchterman-Reingold, Yifan Hu y Noverlap, por medio de la herramienta Gephi, para el análisis y modelado de 30 fan pages de Facebook dedicadas a la promoción de la cultura y divulgación de la ciencia. Los resultados presentan una relación con la dirección y cantidad de aristas por cada uno de los 30 nodos para cada algoritmo aplicado. El enfoque del análisis proporcionó información acerca de la dinámica de la comunidad virtual universitaria, permitiendo una comprensión visual de cómo se conectan y comunican los miembros dentro de la red social Facebook.

Abstract NDVI elucidates the ecophysiological mechanisms faced by vegetation. With the flexibility and versatility of unmanned aerial vehicles (UAVs), temporal, spatial and spectral resolutions have been useful in supporting decision-making. Here, we modeled diurnal change in the NDVI derived from UAV imagery for individual trees in a natural forest stand. Eight flights were conducted during daylight hours over one day to assess the dynamics of the NDVI of the genera Pinus, Juniperus, and Quercus. The results showed unstable NDVI values over time, with a parabolic quadratic trend in the model. The NDVI reached its maximum around 13:00 h and the values differed significantly according to genus: the highest value was found in Pinus with significant differences presented with Juniperus and Quercus, which showed similar values between them (p=0.533). As a validation strategy, we test the model generated using 124 trees independent of those that were sampled, which strengthened our results in terms of reliability. The similarities of statistical parameters confer a high variability of application of the results in models of simulation of similar forests ecosystems. We recommend to study more spectral indices of vegetation, including calibration of the sensor, particularly in longer-term seasonal studies. We conclude that the NDVI measured using UAV should consider image acquisition time to calibrate the records and thus improve the interpretation of results.

Resumen El NDVI dilucida los mecanismos ecofisiológicos a los que se enfrenta la vegetación. Gracias a la flexibilidad y versatilidad de los vehículos aéreos no tripulados (VANT), las resoluciones temporales, espaciales y espectrales han sido útiles para apoyar la toma de decisiones. En este trabajo se modelizó el cambio diurno en el NDVI derivado de imágenes de VANT para árboles individuales en un rodal forestal natural incoetáneo. Se realizaron ocho vuelos durante las horas diurnas a lo largo de un día para evaluar la dinámica del NDVI de los géneros Pinus, Juniperus y Quercus. Los resultados mostraron valores de NDVI inestables en el tiempo, con una tendencia cuadrática parabólica en el modelo. El NDVI alcanzó su máximo alrededor de las 13:00 h y los valores difirieron significativamente según el género: el valor más alto se encontró en Pinus con diferencias significativas presentadas con Juniperus y Quercus, que mostraron valores similares entre ellos (p=0.533). Como estrategia de validación, probamos el modelo generado utilizando 124 árboles independientes de los muestreados, lo que reforzó nuestros resultados en términos de confiabilidad. Las similitudes de los parámetros estadísticos confieren una alta variabilidad de aplicación de los resultados en modelos de simulación de ecosistemas forestales similares. Recomendamos estudiar más índices espectrales de vegetación, incluyendo la calibración del sensor, particularmente en estudios estacionales de más largo plazo. Concluimos que el NDVI medido mediante VANT debe considerar el tiempo de adquisición de la imagen para calibrar los registros y así mejorar la interpretación de los resultados.

Abstract The ecotone is of great importance given the environmental services they provide both to the environment and to human wellbeing. The objective was to know the structure and floristic composition of a contact zone between two types of bushes and also to know the potential it has as a carbon sink. Importance value indices, vertical species distribution, Margalef, Shannon, and true Shannon diversity, as well as biomass, total area, and carbon concentration were determined. Eleven species of vascular plants were recorded, the best represented family was Fabaceae and Cordia boisieri was the species with the highest importance value index. The diversity of the plant community is low according to the Shannon index. The total canopy area was 4 276.03 m2 ha-1, while the vertical index of species defined three height strata. The stored carbon is 2.82 Mg ha-1, while the total aerial biomass was 5 667.15 kg ha-1.

Resumen El ecotono es de gran importancia dado los servicios ambientales que prestan tanto al ambiente como al ser humano. El objetivo fue conocer la estructura y la composición florística en una zona de contacto entre dos tipos de matorrales y conocer además el potencial que tiene como sumidero de carbono. Se determinaron los índices de valor de importancia, distribución vertical de especies, Margalef, Shannon y la diversidad verdadera de Shannon. Además de la biomasa, área total y la concentración de carbono. Se registraron 11 especies de plantas vasculares, la familia mejor representada fue Fabaceae. La especie Cordia boisieri tuvo el mayor índice de valor de importancia. La diversidad de la comunidad vegetal fue baja. El área total de copa fue de 4 276.03 m2 ha-1, con tres estratos de altura. El carbono almacenado fue de 2.82 Mg ha-1, mientras que la biomasa aérea total fue de 567.15 kg ha-1.

Resumen Conocer el contenido de biomasa aérea de árboles individuales es importante en los esfuerzos para determinar la contribución de los bosques en el secuestro de carbono. Los datos LiDAR (Light Detection and Ranging) son una alternativa a las imágenes satelitales para estimar características del arbolado. Dada la importancia de los bosques de la vertiente oriental de la Cuenca del ex Lago de Texcoco para la captura de carbono, el objetivo del presente estudio fue evaluar modelos de regresión para la estimación de biomasa aérea en árboles individuales de Pinus hartwegii Lindl. a partir de datos LiDAR. En campo se midió el diámetro normal (Dn), la altura total (AT), altura de fuste limpio (AF) y el diámetro de copa (DC) de 60 árboles. Sobre un conjunto de datos LiDAR aerotransportados se identificaron esos árboles y se midieron las mismas variables (ATL, AFL y DCL, donde la letra L hace referencia a LiDAR), excepto el diámetro normal, ya que no es posible su medición directa con el LiDAR. Para conocer la biomasa aérea de los árboles se usó el Dn y una ecuación alométrica generada previamente por otros autores. Mediante la técnica de regresión se ajustaron siete modelos lineales y cinco no lineales eligiendo aquel con menor raíz del cuadrado medio del error, mayor R2adj y menor valor del criterio de información de Akaike, además de cumplir con los supuestos de regresión. El modelo seleccionado es de tipo exponencial, con las variables ATL y DCL para estimar la biomasa: RMSE = 406.70, R2adj = 0.8107 y AIC = 723.88.

Summary Knowing the aboveground biomass content of individual trees is important in efforts to determine forests contribution to carbon sequestration. LiDAR (Light Detection and Ranging) data are an alternative to satellite images to estimate tree attributes. Considering the importance of forestland on the eastern slopes of the former Texcoco Lake basin for carbon capture, this study aimed to assess regression models for the estimation of above-ground biomass in individual Pinus hartwegii Lindl. trees from LiDAR data. In the field the breast-height diameter (Dn), total height (AT), stem height (AF) and crown diameter (DC) of 60 trees were measured. Those trees were identified on an air-borne LiDAR dataset and the same variables were measured (namely ATL, AFL and DCL, where L stands for LiDAR), except for Dn since it is not possible to measure it directly from LiDAR. Above-ground tree biomass was determined based on Dn and an allometric equation previously developed by other authors. Using the regression technique seven linear and five nonlinear models were adjusted, choosing the one with the lowest mean square root of the error (RMSE), the largest R2adj and the lowest value of the Akaike information criterion, in addition to the compliance of regression assumptions. The selected model is exponential with ATL and DCL variables to estimate biomass: RMSE = 406.70, R2adj = 0.8107 and AIC = 723.88.

RESUMO: O objetivo desse estudo foi avaliar a resposta sorológica induzida após vacinação com quatro formulações comerciais contendo o BVDV-1, BVDV-2 e BoHV-1 em novilhas jovens com títulos de anticorpos maternos circulantes, assim como analisar uma possível resposta anamnéstica em vacinações, após a completa metabolização dos anticorpos maternais aos seis meses de idade. Foram selecionadas 87 novilhas Holandesas, distribuídas aleatoriamente em quatro grupos experimentais de acordo com as vacinas: vacina A (n = 22), vacina B (n = 22), vacina C (n = 24) e vacina D (n = 19), aplicadas aos dias 60, 90 e 180 de vida. Testes de vírus-neutralização (VN) foram realizados no momento basal (D60) e 30 dias após a aplicação da 2ª dose (D120) e 3ª dose (D210). Observou-se efeito do grupo (P ≤ 0,001), dia da vacinação (P ≤ 0,001), e interação grupo versus dia (P ≤ 0,001) para os títulos de anticorpos produzidos contra o BVDV-1, BVDV-2 e BoHV-1, por meio do comando PROC MIXED (Statistical Analysis System, versão 9,4). Os títulos de anticorpos contra o BVDV-1, BVDV-2 e BoHV-1 eram semelhantes no momento basal aos 60 dias de vida. Os títulos médios de anticorpos foram constantes e persistentes contra o BVDV-1 nas novilhas imunizadas com as vacinas A e C, porém apenas a vacina A teve este mesmo perfil contra o BVDV-2. Em relação ao BoHV-1, os grupos B, C e D apresentaram queda nos títulos de anticorpos do D60 ao D210, enquanto as novilhas do grupo A apresentaram queda do D60 ao D120, com aumento intenso dos títulos no D210. A frequência de animais com títulos acima dos protetores contra o BVDV-1, BVDV-2 e BoHV-1 foram muito baixos ou nulos após as vacinações nos animais imunizados com as formulações B, C e D. Sugere-se que a imunidade passiva interferiu negativamente na indução de anticorpos pelas vacinas, observando-se indicadores de resposta anamnéstica apenas para as novilhas vacinadas com a formulação A. RESUMO BVDV1, BVDV1 BVDV 1, 1 BVDV2 2 BVDV- BoHV1 BoHV BoHV- circulantes idade 8 Holandesas n 22, 22 , 22) 24 19, 19 19) 9 18 vida vírusneutralização vírus neutralização VN (VN (D60 3 ª D120 (D120 D210 . (D210) Observouse Observou se P 0,001, 0001 0,001 0 001 BoHV1, Statistical System 9,4. 94 9,4 4 9,4) 6 BVDV2. 2. D6 Sugerese Sugere observandose observando (D6 D12 (D12 D21 (D210 000 0,00 00 9, (D D1 (D1 D2 (D21 0,0 (D2 0,

ABSTRACT: This study evaluated the vaccine-induced serological response after administering four commercial formulations containing Bovine Viral Diarrhea Virus (BVDV) type-1, BVDV-2, and Bovine Herpesvirus type 1 (BoHV-1) to young heifers with circulating maternal antibody titers. The study also determined the anamnestic response to vaccinations after the complete metabolization of maternal antibodies when the calves reached six months. Eighty-seven Holstein heifers were selected and randomly distributed into four experimental groups based on the vaccine administered. The four vaccine-based experimental groups were as follows: vaccine A (n = 22), vaccine B (n = 22), vaccine C (n = 24), and vaccine D (n = 19), given on the 60th, 90th, and 180th day of life, respectively. Virus-neutralization (VN) tests were performed at baseline (day 60) and 30 days after administering the second (day 120) and third doses (day 210). We analyzed the effect of vaccine group (P ≤ 0.001), day of vaccination (P ≤ 0.001), and group versus vaccine day interaction (P ≤ 0.001) for antibody titers produced against BVDV-1, BVDV-2, and BoHV-1 using the PROC MIXED method (Statistical Analysis System -SAS 9.4). Antibody titers against BVDV-1, BVDV-2, and BoHV-1 were similar at baseline and on day 60 of life. The mean antibody titers were constant and persisted against BVDV-1 in heifers immunized with vaccines A and C. Heifers immunized with vaccine A alone had a similar effect against BVDV-2. Regarding BoHV-1, the antibody titers decreased between days 60 and 210 in groups B, C, and D. The antibody titer for heifers in group A also decreased between days 60 and day 120, and an intense increase in titers was observed on day 210. After being immunized with formulations B, C, and D, the frequency of animals with titers above protective levels for BVDV-1, BVDV-2, and BoHV-1 was very low or null. Indicators of anamnestic response were observed in heifers vaccinated with formulation A only. Therefore, it can be concluded that passive immunity negatively interferes with antibody production after vaccination. ABSTRACT vaccineinduced induced BVDV (BVDV type1, type1 1, type-1 BVDV2, BVDV2 2, 2 BVDV-2 BoHV1 BoHV (BoHV-1 months Eightyseven Eighty seven administered vaccinebased follows n 22, 22 , 22) 24, 24 24) 19, 19 19) 60th th 90th life respectively Virusneutralization neutralization VN (VN 3 120 . 210) P 0.001, 0001 0.001 0 001 BVDV1, BVDV1 BoHV- Statistical SAS 9.4. 94 9.4 9 4 9.4) 6 BVDV- BVDV2. 2. BoHV1, 21 null only Therefore type- (BoHV- 12 000 0.00 00 9. (BoHV 0.0 0.

RESUMO: Enterites infecciosas são altamente prevalentes entre cães em todo o mundo e, em alguns casos, podem ser fatais. Este estudo descreve os achados clínicos e laboratoriais de infecções simples e mistas associadas a enterites graves em 76 cães do sul do Brasil. Segmentos intestinais e/ou fezes foram submetidos à análise patológica e detecção molecular de vírus de genoma DNA, bactérias e protozoários. Lesões intestinais severas foram observadas na maioria dos casos. Infecções únicas foram detectadas em 52,6% dos casos, e infecções duplas (36,8%) e triplas (10,5%) também foram identificadas. O Protoparvovírus carnívoro 2 (CPV-2) foi o agente mais frequente nas infecções simples (36,8% do total). Coinfecção por CPV-2 e Giardia sp. foi a mais comum em infecções duplas (19,7%), seguido por CPV-2 e Cryptosporidium spp. (10,5%). A tripla infecção mais frequente foi CPV-2, Giardia spp. e Cryptosporidium spp. (6,6%). Nossos resultados demostraram que infecções simples e mistas estão associadas a enterites graves em cães no sul do Brasil, envolvendo principalmente CPV-2 e Giardia sp. RESUMO casos fatais 7 Brasil eou ou DNA protozoários 526 52 6 52,6 36,8% 368 36 8 10,5% 105 10 5 (10,5% identificadas CPV2 CPV (CPV-2 (36,8 total. total . total) CPV- sp 19,7%, 197 19,7% , 19 (19,7%) spp 10,5%. CPV2, 2, 6,6%. 66 6,6% (6,6%) 52, 36,8 3 10,5 1 (10,5 (CPV- (36, 19,7 (19,7% 6,6 (6,6% 36, 10, (10, (CPV (36 19, (19,7 6, (6,6 (10 (3 (19, (6, (1 ( (19 (6

ABSTRACT: Infectious enteritis is highly prevalent among dogs worldwide and, in some cases, it can be fatal. This study describes the clinical and laboratorial findings of single and mixed infections associated with severe enteritis in 76 dogs from Southern Brazil. Intestinal segments and/or fecal samples were subjected to histopathology and molecular detection of DNA viruses, bacteria and protozoa. Severe intestinal lesions were observed in most cases. Single infections were detected in 52.6% of cases, double (36.8%) and triple (10.5%) infections were also identified. Carnivore protoparvovirus 2 (CPV-2) was the most frequent agent in single infections (36.8%). Coinfection by CPV-2 and Giardia spp. was the most common in dual infections (19.7%), followed by CPV-2 and Cryptosporidium spp. (10.5%). The most frequent triple infection was CPV-2, Giardia sp. and Cryptosporidium spp. (6.6%). Our results shown that single and mixed infections are associated with severe enteritis in dogs in southern Brazil, mainly involving CPV-2 and Giardia sp. ABSTRACT cases fatal 7 Brazil andor or viruses protozoa 526 52 6 52.6 36.8% 368 36 8 (36.8% 10.5% 105 10 5 (10.5% identified CPV2 CPV (CPV-2 36.8%. . CPV- spp 19.7%, 197 19.7% , 19 (19.7%) 10.5%. CPV2, 2, sp 6.6%. 66 6.6% (6.6%) 52. 36.8 3 (36.8 10.5 1 (10.5 (CPV- 19.7 (19.7% 6.6 (6.6% 36. (36. 10. (10. (CPV 19. (19.7 6. (6.6 (36 (10 (19. (6. (3 (1 (19 (6 (

Abstract The blood bacterial microbiota of the Texas tortoise (Gopherus berlandieri) in Tamaulipas, Mexico, was characterized by next-generation sequencing. In 2019, blood was collected from 6 free-living tortoises. DNA was extracted, the V3-V4 region of the 16S rRNA gene was amplified, and Illumina sequencing was performed. The results showed 9 phyla, 20 classes, 42 orders, 81 families, 176 genera and 299 bacterial species. Firmicutes was the most abundant phylum in the blood of G. berlandieri; this taxon has been recorded as predominant in the intestine, excrement, nasal exudates and saliva of other species of the genus Gopherus. The dominant bacterial genera were Caldalkalibacillus, Anaerobacillus, Nesterenkonia and Bacillus. These taxa have been recorded in alkaline and halophilic soils, such as those found in G. berlandieri burrows. All of these bacterial taxa can enter the bloodstream of G. berlandieri via intestinal, oral and nasal translocation. Likewise, 3 bacterial taxa (Coxiella sp., Ehrlichia sp. and Anaplasma phagocytophilum) that are transmitted by arthropod vectors, as well as the potentially pathogenic Salmonella enterica were recorded. This information is the first bacteriological reference for the blood of G. berlandieri, and is expected to be useful for health and conservation programs.

Resumen La microbiota bacteriana sanguínea de la tortuga texana (Gopherus berlandieri) en Tamaulipas, México, fue caracterizada mediante secuenciación de siguiente generación. En 2019 se colectó sangre de 6 tortugas silvestres. Se extrajo el DNA, se amplificó la región V3-V4 del gen 16S rRNA y se realizó secuenciación con Illumina. Los resultados mostraron 9 phyla, 20 clases, 42 órdenes, 81 familias, 176 géneros y 299 especies bacterianas. Firmicutes fue el phylum más abundante en la sangre de G. berlandieri; este taxón ha sido registrado como predominante en intestino, excremento, exudados nasales y saliva de otras especies de Gopherus. Los géneros bacterianos dominantes fueron Caldalkalibacillus, Anaerobacillus, Nesterenkonia y Bacillus. Estos taxa se encuentran en suelos alcalinos y halófilos como los que se presentan en las madrigueras de G. berlandieri. Todos estos taxa bacterianos podrían ingresar al torrente sanguíneo vía translocación intestinal, oral y nasal. En la sangre de esta tortuga se registraron 3 taxa (Coxiella sp., Ehrlichia sp. y Anaplasma phagocytophilum) que se trasmiten por artrópodos y el patógeno potencial Salmonella enterica. Esta información es una primera referencia bacteriológica de la sangre de G. berlandieri y se espera que sea de utilidad en programas de salud y conservación.

Resumo Este estudo avaliou a capacidade antibacteriana intratubular de diferentes irrigações ativadas após o preparo químico-mecânico. Setenta e dois canais palatinos de molares superiores foram contaminados com Enterococcus faecalis por 4 semanas, e então a coleta bacteriana inicial do canal principal foi realizada. Os canais foram preparados com WaveOne Gold large (45/.05) e distribuídos em 6 grupos de acordo com a ativação da irrigação final: ativação ultrassônica (UA), XP-Endo Finisher (25/.00), XP Clean (25/.02), EasyClean (25/.04) em movimento reciprocante e em movimento rotatório contínuo (ECRot) e irrigação convencional. Após a irrigação final, outra coleta bacteriana do canal principal foi realizada, e a raiz foi seccionada transversalmente em três terços e corada para análise por microscopia laser confocal. Bactérias intra tubulares foram coletadas através de pó de dentina e cultivadas para análise de viabilidade bacteriana. As comparações entre grupos e intragrupos foram realizadas usando análise de variância e análise de variância de medidas repetidas, respectivamente, ambas com 5% de significância. ECRot apresentou maior capacidade antibacteriana que UA (p<0,05), e ambos foram superiores aos demais grupos (p<0,05) em ambas as metodologias. Pode-se concluir que a ativação da irrigação final melhora a desinfecção do sistema de canais radiculares, e os ativadores têm eficácias diferentes. químicomecânico. químicomecânico químico mecânico. mecânico químico-mecânico semanas realizada 45/.05 4505 45 05 (45/.05 UA, , (UA) XPEndo Endo 25/.00, 2500 25/.00 25 00 (25/.00) 25/.02, 2502 25/.02 02 (25/.02) 25/.04 2504 04 (25/.04 (ECRot convencional confocal repetidas respectivamente 5 significância p<0,05, p005 p p<0,05 0 (p<0,05 metodologias Podese Pode se radiculares 45/.0 450 (45/.0 (UA 250 25/.0 2 (25/.00 (25/.02 (25/.0 p00 p<0,0 (p<0,0 45/. (45/. 25/. (25/. p0 p<0, (p<0, 45/ (45/ 25/ (25/ p<0 (p<0 (45 (25 p< (p< (4 (2 (p (

Abstract This study assessed the intratubular antibacterial ability of different activated irrigations after chemical mechanical preparation. Seventy-two palatal root canals of upper molars were infected with Enterococcus faecalis for 4 weeks, and then initial bacterial collection from the main root canal was performed. The root canals were prepared by using a WaveOne Gold large (45/.05) and distributed into 6 groups according to the activation of the final irrigation: ultrasonic activation (UA), XP-Endo Finisher (25/.00), XP Clean (25/.02), EasyClean (25/.04) in reciprocating motion and continuous rotary motion (ECRot), and conventional irrigation. After final irrigation, another bacterial collection from the main root canal was performed, and the root was sectioned transversely in three-thirds and stained for analysis by confocal laser microscopy. Intratubular bacteria were collected through dentin powder and plated for bacterial viability analysis. Intergroup and intragroup comparisons were performed by using analysis of variance and repeated measures analysis of variance, respectively, both at 5% significance. ECRot had higher antibacterial ability than UA (p<0.05), and both were superior to the other groups (p<0.05) in both methodologies. It can be concluded that activation of final irrigation enhances the disinfection of the root canal system, and activators have different efficacies preparation Seventytwo Seventy two weeks 45/.05 4505 45 05 (45/.05 UA, , (UA) XPEndo Endo 25/.00, 2500 25/.00 25 00 (25/.00) 25/.02, 2502 25/.02 02 (25/.02) 25/.04 2504 04 (25/.04 ECRot, (ECRot) threethirds three thirds microscopy respectively 5 significance p<0.05, p005 p p<0.05 0 (p<0.05 methodologies system 45/.0 450 (45/.0 (UA 250 25/.0 2 (25/.00 (25/.02 (25/.0 (ECRot p00 p<0.0 (p<0.0 45/. (45/. 25/. (25/. p0 p<0. (p<0. 45/ (45/ 25/ (25/ p<0 (p<0 (45 (25 p< (p< (4 (2 (p (

RESUMO As condições ambientais do México permitem a presença de várias espécies de bambu, onde, devido ao seu rápido crescimento e características, os usos comerciais se diversificaram. As Guaduas mexicanas são exemplares com características estruturais exemplares, porém não há informações cartográficas no México que permitam localizar e dimensionar as áreas onde estão ou podem estar localizadas espécies de bambu, o que restringe a tomada de decisão para plantações florestais comerciais. Portanto, pretende-se determinar a probabilidade de ocorrência das 7 espécies deste gênero de bambu, com base na abordagem de máxima entropia e três arranjos de 21 variáveis ambientais, para as quais foram utilizados 478 registros de presença. Todos os modelos gerados definiram um bom ajuste com os dados de treinamento, com um valor de AUC maior que 0,90. Verificou-se que a distribuição de nicho das espécies mexicanas de Guadua é influenciada principalmente pela altitude (ELEV), por isso são encontradas em áreas próximas às regiões costeiras. Da mesma forma, a precipitação anual (BIO12) destacou-se na definição da distribuição deste gênero, assim como a evapotranspiração (ETP) e a temperatura média anual (BIO1). Isso foi independente dos três arranjos de variáveis ambientais que foram testados. bambu diversificaram Portanto pretendese pretende 2 47 treinamento 090 0 90 0,90 Verificouse Verificou ELEV, ELEV , (ELEV) costeiras forma BIO12 BIO (BIO12 destacouse destacou ETP (ETP BIO1. BIO1 . (BIO1) testados 4 09 9 0,9 (ELEV (BIO1 0, (BIO

ABSTRACT The environmental conditions of Mexico allow the presence of several species of bamboo, where commercial uses have diversified due to their rapid growth and characteristics. The Mexican Guaduas are specimens with exemplary structural characteristics, however, there is no cartographic information in Mexico that allows locating and sizing the areas where bamboo species are located or can be located, which restricts decision making for commercial forest plantations. Therefore, it is intended to determine the probability of occurrence of the 7 species of this genus of bamboo, based on the maximum entropy approach and three arrangements of 21 environmental variables, for which 478 presence records were used. All generated models defined a good fit with the training data, with an AUC value greater than 0.90. It was found that the niche distribution of Guadua mexicana species is mainly influenced by altitude (ELEV), so they are found in areas close to coastal regions. Likewise, in defining the distribution of this genus, annual precipitation (BIO12), evapotranspiration (ETP) and average annual temperature (BIO1) stood out. This was independent of the three arrangements of environmental variables that were tested. characteristics however plantations Therefore 2 47 used data 090 0 90 0.90 ELEV, ELEV , (ELEV) regions Likewise BIO12, BIO12 BIO (BIO12) ETP (ETP BIO1 (BIO1 out tested 4 09 9 0.9 (ELEV (BIO12 (BIO 0.

ABSTRACT Background: university social responsibility constitutes an academic management approach that promotes sustainable social development. Objective: to analyze knowledge management in the Department of Basic Biomedical Sciences, of the "Salvador Allende" Faculty of Medical Sciences, from 2018 to 2022, based on university social responsibility. Methods: a descriptive investigation was developed, with a qualitative approach, complementing the methodologies of the case study and the constructivist grounded theory. Theoretical methods were used: analysis-synthesis, induction-deduction, ascent from the abstract to the concrete and historical logic; and empirical ones: documentary review, content analysis and focal group. Results: the analysis carried out showed that knowledge management in the Department of Basic Biomedical Sciences, of the aforementioned faculty, has a strategic approach and has had a positive educational impact as a result of organizational learning, and it´s linked to academic management. Conclusions: the departmental knowledge management determined an update of the organizational culture, causing a favorable social impact, mediated by an educational intervention tributary of a relevant professional training.

RESUMEN Fundamento: la responsabilidad social universitaria constituye un enfoque de gestión académica promotor del desarrollo social sostenible. Objetivo: analizar la gestión del conocimiento en el departamento de Ciencias Básicas Biomédicas, de la Facultad de Ciencias Médicas “Salvador Allende”, durante los años 2018 al 2022, sobre la base de la responsabilidad social universitaria. Métodos: se desarrolló una investigación descriptiva, con enfoque cualitativo, complementando las metodologías del estudio de caso y de la teoría fundamentada constructivista. Se utilizaron métodos teóricos: análisis-síntesis, inducción-deducción, ascenso de lo abstracto a lo concreto e histórico lógico; y empíricos: revisión documental, análisis de contenido y grupo focal. Resultados: el análisis realizado evidenció que la gestión del conocimiento en el departamento de Ciencias Básicas Biomédicas, de la referida facultad, ostenta un enfoque estratégico y ha tenido un impacto educativo positivo como resultado del aprendizaje organizacional, y está vinculado con la gestión académica. Conclusiones: la gestión del conocimiento departamental determinó una actualización de la cultura organizacional, causante de un impacto social favorable, mediado por una intervención educativa tributaria de una formación profesional pertinente.

Resumo O objetivo deste trabalho foi avaliar parâmetros físicos e químicos de frutos de novos genótipos de morangueiro em condições de campo. O estudo foi realizado durante as safras de 2018 e 2019. Foram avaliados 12 genótipos do programa de melhoramento genético da Embrapa e duas cultivares comerciais (Camino Real e Merced). Os parâmetros avaliados foram: número médio de frutos, massa média de frutos, sólidos solúveis, acidez titulável e relação sólidos solúveis/acidez titulável. Entre os genótipos de morango estudados, as seleções 8-1, 35-22 ('BRS DC22'), 31-13, 7-5 e 32-2 produzem mais frutos por planta. Entre os novos genótipos, 'BRS DC25' (Fénix) e 31-13 apresentam a melhor massa média de frutos nos dois períodos avaliados. O maior teor de açúcar é observado nos genótipos 35-22 ('BRS DC22') e 65-2, enquanto 8-1 e 'BRS DC25' (Fênix) apresentam a maior acidez titulável. Os genótipos 7-5, 35-22 ('BRS DC22'), 34-2 e 35-6 apresentaram a melhor relação sólidos solúveis/acidez. campo 201 2019 1 Camino Merced. Merced . Merced) foram solúveis solúveisacidez estudados 81, 81 8 1, 3522 35 22 35-2 BRS DC22, DC22 DC , 3113, 3113 31 13, 13 75 7 5 7- 322 32 2 32- planta DC25 Fénix (Fénix 31-1 DC22' 652, 652 65 2, 65-2 8- Fênix (Fênix 75, 5, 342 34 34- 356 6 35- 20 352 3 DC2 311 31- 65-

Abstract The objective of this work was to evaluate physical and chemical fruit parameters of new strawberry genotypes underfield conditions. The study was carried out during the 2018 and 2019 seasons. Twelve genotypes from the breeding program of Embrapa and two commercial cultivars (Camino Real and Merced) were evaluated. The assessed parameters were: average fruit number, average fruit weight, soluble solids, titrable acidity, and the soluble solids/titrable acidity ratio. Among the studied strawberry genotypes, the 8-1, 35-22 ('BRS DC22'), 31-13, 7-5, and 32-2 selections produce more fruits per plant. Among the new genotypes, 'BRS DC25' (Fênix) and 31-13 present the best average fruit weight in both evaluated seasons. The highest sugar content is observed in genotypes 35-22 ('BRS DC22') and 65-2, whereas 8-1 and 'BRS DC25' (Fênix) show the highest acidity. The 7-5, 35-22 ('BRS DC22'), 34-2, and 35-6 genotypes present the best soluble solid/acidity ratio. conditions 201 seasons Camino Merced number solids solidstitrable ratio 81, 81 8 1, 1 3522 35 22 35-2 BRS DC22, DC22 DC , 3113, 3113 31 13, 13 75, 75 7 5, 5 7-5 322 32 2 32- plant DC25 Fênix (Fênix 31-1 DC22' 652, 652 65 2, 65-2 8- 342, 342 34 34-2 356 6 35- solidacidity solid 20 352 3 DC2 311 7- 31- 65- 34-

Resumo O objetivo deste trabalho foi estimar a produtividade relativa que maximiza a rentabilidade da lavoura de soja rotacionada com arroz irrigado por inundação. Para tanto, 13 áreas (de 2,6 a 5,2 ha) de alta produção em sistemas soja-arroz, em terras baixas, na região Sul do Brasil foram selecionadas antes da semeadura. O cálculo dos custos de produção incluiu sementes, tratamento de sementes, fertilizantes, calcário, defensivos agrícolas, irrigação, terra, operações terceirizadas, mão de obra e consumo de combustível. A produtividade observada foi transformada em produtividade relativa, ao se multiplicar o quociente da produtividade observada pelo potencial produtivo estimado pelo modelo CSM-CROPGRO-Soybean. A produtividade hídrica foi calculada como a razão entre a produtividade observada e a água disponível durante o ciclo da cultura. O potencial de produtividade variou de 6,1 a 7,4 Mg ha−1, enquanto a produtividade relativa variou de 45,3 a 101,2%. Além disso, os custos variaram de US$564,86 a US$1.122,86 por hectare, e a rentabilidade de US$767,18 ha−1 a US$3.149,75 por hectare. A maior rentabilidade da lavoura de soja em rotação com arroz irrigado por inundação ocorre com produtividade relativa entre 67 e 84%. tanto 1 26 2 6 2, 52 5 5, ha sojaarroz, sojaarroz arroz, soja-arroz baixas semeadura sementes fertilizantes calcário agrícolas irrigação terra terceirizadas combustível CSMCROPGROSoybean. CSMCROPGROSoybean CSM CROPGRO Soybean. Soybean CSM-CROPGRO-Soybean cultura 61 6, 74 7 4 7, ha1 453 45 3 45, 1012 101 101,2% disso US56486 US 564 86 US$564,8 US112286 122 US$1.122,8 hectare US76718 767 18 US$767,1 ha− US314975 149 75 US$3.149,7 84 84% 10 101,2 US5648 56 8 US$564, US11228 12 US$1.122, US7671 76 US$767, US31497 14 US$3.149, 101, US564 US$564 US1122 US$1.122 US767 US$767 US3149 US$3.149 US56 US$56 US112 US$1.12 US76 US$76 US314 US$3.14 US5 US$5 US11 US$1.1 US7 US$7 US31 US$3.1 US$ US1 US$1. US3 US$3. US$1 US$3

Abstract The objective of this work was to estimate the relative yield that maximizes the profitability of the soybean crop in rotation with flood-irrigated rice. For this, 13 high-yield areas (from 2.6 to 5.2 ha) in lowland soybean-rice systems in Southern Brazil were selected before sowing. The calculation of production costs included seeds, seed treatment, fertilizers, lime, pesticides, irrigation, land, operational outsourcing, labor, and fuel consumption. The observed yield was transformed into relative yield by multiplying the quotient of the observed yield by the yield potential estimated by the CSM-CROPGRO-Soybean model. Water productivity was calculated as the ratio between the observed yield and available water during the crop cycle. Yield potential ranged from 6.1 to 7.4 Mg ha−1, whereas relative yield ranged from 45.3 to 101.2%. In addition, costs ranged from US$564.86 to US$1,122.86 per hectare, and profitability from US$767.18 to US$3,149.75 per hectare. The highest profitability of the soybean crop in rotation with flood-irrigated rice occurs with a relative yield between 67 and 84%. floodirrigated flood irrigated 1 highyield high 26 2 6 2. 52 5 5. ha soybeanrice sowing seeds treatment fertilizers lime pesticides irrigation land outsourcing labor consumption CSMCROPGROSoybean CSM CROPGRO Soybean model cycle 61 6. 74 7 4 7. ha1 ha−1 453 45 3 45. 1012 101 101.2% addition US56486 US 564 86 US$564.8 US112286 122 US$1,122.8 hectare US76718 767 18 US$767.1 US314975 149 75 US$3,149.7 84 84% ha− 10 101.2 US5648 56 8 US$564. US11228 12 US$1,122. US7671 76 US$767. US31497 14 US$3,149. 101. US564 US$564 US1122 US$1,122 US767 US$767 US3149 US$3,149 US56 US$56 US112 US$1,12 US76 US$76 US314 US$3,14 US5 US$5 US11 US$1,1 US7 US$7 US31 US$3,1 US$ US1 US$1, US3 US$3, US$1 US$3