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au:Farias, Antonio Roberto Gomes de

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Comparative genomics and phylogenomics of the Ralstonia solanacearum Moko ecotype and its symptomatological variants
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Pais, Ana Karolina Leite

; Santos, Leandro Victor Silva dos

; Albuquerque, Greecy Mirian Rodrigues

; Farias, Antonio Roberto Gomes de

; Silva Junior, Wilson José

; Balbino, Valdir de Queiroz

; Silva, Adriano Márcio Freire

; Gama, Marco Aurelio Siqueira da

; Souza, Elineide Barbosa de

Genetics and Molecular Biology

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2022, Volume 45 N. 4 elocation e20220038

Abstract: En | Text: En | PDF: En

Abstract Banana tree bacterial wilt is caused by the Ralstonia solanacearum Moko ecotype. These strains vary in their symptom progression in banana, and are classified as typical Moko variants (phylotype IIA and IIB strains from across Central and South America), Bugtok variant (Philippines), and Sergipe facies (the states of Sergipe and Alagoas, Brazil). This study used comparative genomic and phylogenomic approaches to identify a correlation between the symptom progression of the Moko ecotypes based on the analysis of 23 available genomes. Average nucleotide identity and in silico DNA-DNA hybridization revealed a high correlation (>96% and >78%, respectively) between the genomes of Moko variants. Pan-genome analysis identified 21.3% of inheritable regions between representatives of the typical Moko and Sergipe facies variants, which could be traced to an abundance of exclusive homolog clusters. Moko ecotype genomes shared 1,951 orthologous genes, but representatives with typical symptoms did not display unique orthologues. Moreover, Bugtok disease and Sergipe facies genomes did not share any unique genes, suggesting convergent evolution to a shared symptom progression. Overall, genomic and phylogenomic analyses were insufficient to differentiate the Moko variants based on symptom progression.

https://doi.org/10.1590/1678-4685-gmb-2022-0038

Genomic sequencing of different sequevars of Ralstonia solanacearum belonging to the Moko ecotype
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Pais, Ana Karolina Leite

; Silva, Jessica Rodrigues da

; Santos, Leandro Victor Silva dos

; Albuquerque, Greecy Mirian Rodrigues

; Farias, Antonio Roberto Gomes de

; Silva Junior, Wilson José

; Balbino, Valdir de Queiroz

; Silva, Adriano Márcio Freire

; Gama, Marco Aurélio Siqueira da

; Souza, Elineide Barbosa de

Genetics and Molecular Biology

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2021, Volume 44 N. 1 elocation e20200172

Abstract: En | Text: En | PDF: En

Abstract Banana vascular wilt or Moko is a disease caused by Ralstonia solanacearum. This study aimed to sequence, assemble, annotate, and compare the genomes of R. solanacearum Moko ecotypes of different sequevar strains from Brazil. Average nucleotide identity analyses demonstrated a high correlation (> 96%) between the genome sequences of strains CCRMRs277 (sequevar IIA-24), CCRMRs287 (IIB-4), CCRMRs304 (IIA-24), and CCRMRsB7 (IIB-25), which were grouped into phylotypes IIA and IIB. The number of coding sequences present in chromosomes and megaplasmids varied from 3,070 to 3,521 and 1,669 to 1,750, respectively. Pangenome analysis identified 3,378 clusters in the chromosomes, of which 2,604 were shared by all four analyzed genomes and 2,580 were single copies. In megaplasmids, 1,834 clusters were identified, of which 1,005 were shared by all four genomes and 992 were identified as single copies. Strains CCRMRsB7 and CCRMRs287 differed from the others by having unique clusters in both their chromosomes and megaplasmids, and CCRMRsB7 possessed the largest genome among all Moko ecotype strains sequenced to date. Therefore, the genomic information obtained in this study provides a theoretical basis for the identification, characterization, and phylogenetic analysis of R. solanacearum Moko ecotypes.

https://doi.org/10.1590/1678-4685-gmb-2020-0172 901 downloads

Microstructural and mechanical characterisation of the Simon Bolivar's iron bridge structure, 19th century, Arequipa, Peru
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Mamani-Calcina, Elmer Antonio

; Apaza-Huallpa, Edgar

; Gonzalez-Diaz, Daysi

; Vargas-Cardenas, Hector

; Guerra-Santander, Erika

; Andrade-Centeno, Dany Michell

; Nacsa, Bruno

; Alves, Jonathan Paulino Ferreira

; Landgraf, Fernando José Gomes

; Azevedo, Cesar Roberto de Farias

REM - International Engineering Journal

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dec 2020, Volume 73 N. 4 Pages 523 - 530

Abstract: En | Text: En | PDF: En

Abstract The microstructure of the main structural elements (Phoenix columns, transversal beams and tie-rods) of the Simón Bolivar iron bridge, located in Arequipa (Peru), was investigated. The bridge was supposedly designed by Gustave Eiffel and opened to the public in 1882. The characterisation revealed that the columns, beams and tie-rods are made of puddled iron (ferritic matrix of equiaxial grains and numerous slag inclusions). The tensile properties were estimated by hardness and nanohardness measurements, and the tensile strength varied between 285 and 390 MPa and the Young's modulus from 187 to 198 GPa. The components of puddled iron with the same provenance, Phoenix Iron Company, showed typical variations in the chemical composition and microstructure of the slag inclusions. The microanalysis results of the slag inclusions indicated that the wüstite phase presented qualitatively the same composition with pronounced variations in the ratio between V2O5 and TiO2 contents, confirming that a single parameter cannot be used to determine the provenance of iron artefacts. The intense presence of slag inclusions in the puddled iron components (~ 10% in volume fraction) heterogeneously distributed in the iron illustrates the technological limits of the iron refining technique of the time.

https://doi.org/10.1590/0370-44672020730039 801 downloads

O panorama da vigilância de eventos adversos pós-vacinação ao fim da década de 2010: importância, ferramentas e desafios
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Oliveira, Patrícia Mouta Nunes de

; Lignani, Letícia Kegele

; Conceição, Deborah Araújo da

; Farias, Paula Molinari Cardoso de Mello

; Takey, Paulo Roberto Gomes

; Maia, Maria de Lourdes de Sousa

; Camacho, Luiz Antonio Bastos

Cadernos de Saúde Pública

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2020, Volume 36 Suppl. 2 elocation e00182019

Abstract: Pt En Es | Text: Pt En Es | PDF: Pt | PDF: En | PDF: Es

A vacinação é uma das ações mais efetivas para proteger o indivíduo e a população contra doenças imunopreveníveis. Vacinas são produtos seguros e eficazes, porém, como qualquer outro medicamento, podem causar eventos adversos, que ganham maior visibilidade na medida em que as doenças são controladas, eliminadas ou erradicadas. Este trabalho analisou as ações de vigilância de eventos adversos pós-vacinação (EAPV) com base em dados da literatura científica e sites de programas de imunizações, agências reguladoras e de saúde, além da expertise dos autores nas áreas de imunizações e farmacovigilância. Com o aumento do número de vacinas no calendário básico e a ampliação do acesso da população, tornou-se fundamental o estabelecimento de um sistema eficiente de vigilância de EAPV no Brasil. Entretanto, a subnotificação de casos no Brasil e em outros países dificulta a detecção de EAPV, principalmente os raros. Informações sempre atualizadas sobre o benefício/risco das vacinas permitem que programas de imunizações deem respostas rápidas e claras aos rumores de EAPV. Isso garante a confiabilidade no sistema, ainda mais diante do crescente movimento antivacinista e a influência cada vez maior das mídias sociais na opinião pública.

Immunization is one of the most effective measures to protect individuals and the population against vaccine-preventable diseases. Vaccines are safe and effective products, but like any other drug they can cause adverse events, which tend to become more visible as the diseases are controlled, eliminated, or eradicated. This study analyzed activities in the surveillance of adverse events following immunization (AEFI) based on data from the scientific literature, websites of immunization programs and health andregulatory agencies, and the authors’ expertise in the areas of immunizations and pharmacovigilance. With the increase in the number of vaccines in the basic immunization schedule and expansion of the population’s access, it has become essential to establish an efficient surveillance system for AEFI in Brazil. However, underreporting of cases in Brazil and in other countries hinders the detection of AEFI, especially rare events. Constantly updated information on vaccines’ risks and benefits allows immunization programs to provide rapid and clear responses to rumors of AEFI. This ensures the system’s reliability, especially in the face of the growing antivaccine movement and the increasing influence of social media in public opinion.

La vacunación es una de las acciones más efectivas para proteger al individuo y a la población contra enfermedades inmunoprevenibles. Las vacunas son productos seguros y eficaces, sin embargo, como cualquier otro medicamento, pueden causar eventos adversos, que tienen mayor visibilidad según se controlen, eliminen o se erradiquen las enfermedades. Este trabajo analizó las acciones de vigilancia de eventos adversos posvacunación (EAPV), basándose en datos de la literatura científica y sitios web de programas de inmunizaciones, agencias reguladoras y de salud, además de la expertise de los autores en las áreas de inmunizaciones y farmacovigilancia. Con el aumento del número de vacunas en el calendario básico y la ampliación del acceso de la población, se hizo fundamental el estabelecimiento de un sistema eficiente de vigilancia de EAPV en Brasil. Sin embargo, la subnotificación de casos en Brasil y en otros países dificulta la detección de EAPV, principalmente, los raros. Informaciones siempre actualizadas sobre el beneficio/riesgo de las vacunas permiten que programas de inmunizaciones proporcionen respuestas rápidas y claras a los rumores sobre EAPV. Esto garantiza la confianza en el sistema, incluso más aún ante el creciente movimiento antivacunas y la influencia cada vez mayor de los redes sociales en la opinión pública.

https://doi.org/10.1590/0102-311x00182019 1945 downloads

O panorama da vigilância de eventos adversos pós-vacinação ao fim da década de 2010: importância, ferramentas e desafios
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Oliveira, Patrícia Mouta Nunes de

; Lignani, Letícia Kegele

; Conceição, Deborah Araújo da

; Farias, Paula Molinari Cardoso de Mello

; Takey, Paulo Roberto Gomes

; Maia, Maria de Lourdes de Sousa

; Camacho, Luiz Antonio Bastos

Cadernos de Saúde Pública

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2020, Volume 36 Suppl. 2 elocation e00182019

Abstract: Pt En Es | Text: Pt En Es | PDF: Pt | PDF: En | PDF: Es

https://doi.org/10.1590/0102-311x00182019

UPDATE OF THE BRAZILIAN SOCIETY OF HEPATOLOGY RECOMMENDATIONS FOR DIAGNOSIS AND MANAGEMENT OF AUTOIMMUNE DISEASES OF THE LIVER
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COUTO, Cláudia Alves

; TERRABUIO, Debora Raquel Benedita

; CANÇADO, Eduardo Luiz Rachid

; PORTA, Gilda

; LEVY, Cynthia

; SILVA, Antônio Eduardo Benedito

; BITTENCOURT, Paulo Lisboa

; CARVALHO FILHO, Roberto José de

; CHAVES, Dalton Marques

; MIURA, Irene Kazue

; CODES, Liana

; FARIA, Luciana Costa

; EVANGELISTA, Andreia Silva

; FARIAS, Alberto Queiroz

; GONÇALVES, Luciana Lofêgo

; HARRIZ, Michelle

; LOPES, Edmundo Pessoa de Almeida

; LUZ, Gustavo Oliveira

; OLIVEIRA, Patrícia Marinho Costa

; OLIVEIRA, Elze Maria Gomes

; SCHIAVON, Janaina Luz Narciso

; SEVÁ-PEREIRA, Tiago

Arquivos de Gastroenterologia

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jun 2019, Volume 56 N. 2 Pages 232 - 241

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO Desde a publicação em 2015 das recomendações da Sociedade Brasileira de Hepatologia sobre a prevenção e tratamento de doenças hepáticas autoimunes, novos dados baseados em evidências científicas foram publicados na literatura, mudando o diagnóstico e tratamento da hepatite autoimune (HAI), colangite biliar primária (CBP), colangite esclerosante primária (CEP), síndromes de sobreposição de HAI, CBP e CEP e o manejo de complicações específicas além de outros tópicos relativos à HAI e doenças hepáticas colestáticas. Este manuscrito atualiza as recomendações anteriores de acordo com as melhores evidências disponíveis na literatura até o momento. O mesmo painel de experts que participou da primeira diretriz revisou todas as recomendações de acordo com os dados publicados na literatura e elaborou um manuscrito submetido subsequentemente à apreciação e revisão de todos os membros da Sociedade Brasileira de Hepatologia via homepage da sociedade. As recomendações finais atualizadas foram condensadas no presente documento.

ABSTRACT New data concerning the management of autoimmune liver diseases have emerged since the last single-topic meeting sponsored by the Brazilian Society of Hepatology to draw recommendations about the diagnosis and treatment of autoimmune hepatitis (AIH), primary biliary cholangitis (PBC), primary sclerosing cholangitis (PSC), overlap syndromes of AIH, PBC and PSC and specific complications and topics concerning AIH and cholestatic liver diseases. This manuscript updates those previous recommendations according to the best evidence available in the literature up to now. The same panel of experts that took part in the first consensus document reviewed all recommendations, which were subsequently scrutinized by all members of the Brazilian Society of Hepatology using a web-based approach. The new recommendations are presented herein.

https://doi.org/10.1590/s0004-2803.201900000-43 3841 downloads

Investigating the Provenance of Iron Artifacts of the Royal Iron Factory of São João de Ipanema by Hierarchical Cluster Analysis of EDS Microanalyses of Slag Inclusions
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Mamani-Calcina, Elmer Antonio

; Landgraf, Fernando José Gomes

; Azevedo, Cesar Roberto de Farias

Materials Research

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feb 2017, Volume 20 N. 1 Pages 119 - 129

Abstract: En | Text: En | PDF: En

Microstructural characterization techniques, including EDX (Energy Dispersive X-ray Analysis) microanalyses, were used to investigate the slag inclusions in the microstructure of ferrous artifacts of the Royal Iron Factory of São João de Ipanema (first steel plant of Brazil, XIX century), the D. Pedro II Bridge (located in Bahia, assembled in XIX century and produced in Scotland) and the archaeological sites of São Miguel de Missões (Rio Grande do Sul, Brazil, production site of iron artifacts, the XVIII century) and Afonso Sardinha (São Paulo, Brazil production site of iron artifacts, XVI century). The microanalyses results of the main microconstituents of the microstructure of the slag inclusions were investigated by hierarchical cluster analysis and the dendrogram with the microanalyses results of the wüstite phase (using as critical variables the contents of MnO, MgO, Al2O3, V2O5 and TiO2) allowed the identification of four clusters, which successfully represented the samples of the four investigated sites (Ipanema, Sardinha, Missões and Bahia). Finally, the comparatively low volumetric fraction of slag inclusions in the samples of Ipanema (~1%) suggested the existence of technological expertise at the ironmaking processing in the Royal Iron Factory of São João de Ipanema.

https://doi.org/10.1590/1980-5373-mr-2016-0444 1340 downloads

Brazilian society of hepatology recommendations for the diagnosis and management of autoimmune diseases of the liver
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Bittencourt, Paulo Lisboa

; Cançado, Eduardo Luiz Rachid

; Couto, Cláudia Alves

; Levy, Cynthia

; Porta, Gilda

; Silva, Antônio Eduardo Benedito

; Terrabuio, Debora Raquel Benedita

; Carvalho Filho, Roberto José de

; Chaves, Dalton Marques

; Miura, Irene Kazue

; Codes, Liana

; Faria, Luciana Costa

; Evangelista, Andreia Silva

; Farias, Alberto Queiroz

; Gonçalves, Luciana Lofêgo

; Harriz, Michele

; Lopes Neto, Edmundo Pessoa A

; Luz, Gustavo Oliveira

; Oliveira, Patrícia

; Oliveira, Elze Maria Gomes de

; Schiavon, Janaina Luz Narciso

; Seva-Pereira, Tiago

; Parise, Edison Roberto

Arquivos de Gastroenterologia

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dec 2015, Volume 52 Suppl. 1 Pages 15 - 46

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO Para definir as recomendações baseadas em evidências científicas sobre o diagnóstico e tratamento das doenças autoimnus do fígado, a Sociedade Brasileira de Hepatologia organizou em Outubro de 2014, encontro monotemático em São Paulo. Um Comitê organizador de sete investigadores foi selecionado pela Diretoria da Sociedade para organizar a agenda científica, assim como para selecionar vinte debatedores para fazer uma revisão sistemática e apresentar tópicos relacionados à hepatite autoimune, colangite esclerosante primária, cirrose biliar primária e suas síndromes de superposição (overlap). O texto inicial do submetidoo a apreciação e aprovação da Sociedade Brasileira de Hepatologia através de consulta a todos associados através da home page da Sociedade, O trabalho apresentado representa a versão final do trabalho original, devidamente revisado e organizado em tópicos, segundo as recomendações da Sociedade Brasileira de Hepatologia.

ABSTRACT In order to draw evidence-based recommendations concerning the management of autoimmune diseases of the liver, the Brazilian Society of Hepatology has sponsored a single-topic meeting in October 18th, 2014 at São Paulo. An organizing committee comprised of seven investigators was previously elected by the Governing Board to organize the scientific agenda as well as to select twenty panelists to make a systematic review of the literature and to present topics related to the diagnosis and treatment of autoimmune hepatitis, primary sclerosing cholangitis, primary biliary cirrhosis and their overlap syndromes. After the meeting, all panelists gathered together for the discussion of the topics and the elaboration of those recommendations. The text was subsequently submitted for suggestions and approval of all members of the Brazilian Society of Hepatology through its homepage. The present paper is the final version of the reviewed manuscript organized in topics, followed by the recommendations of the Brazilian Society of Hepatology.

https://doi.org/10.1590/s0004-28032015000500002 8104 downloads

Intensive care management of patients with liver disease: proceedings of a single-topic conference sponsored by the Brazilian Society of Hepatology
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Bittencourt, Paulo Lisboa

; Terra, Carlos

; Parise, Edison Roberto

; Farias, Alberto Queiroz

; Arroyo, Vincent

; Fernandez, Javier

; Pereira, Gustavo

; Maubouisson, Luiz Marcelo

; Andrade, Guilherme Marques

; Costa, Fernando Gomes de Barros

; Codes, Liana

; Andrade, Antônio Ricardo

; Matos, Angelo

; Torres, André

; Couto, Fernanda

; Zyngier, Ivan

Arquivos de Gastroenterologia

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dec 2015, Volume 52 Suppl. 1 Pages 55 - 72

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO A sobrevida de pacientes cirróticos críticos aumentou significantemente nos últimos anos devido a inúmeros avanços obtidos no manejo do paciente com cirrose descompensada e com insuficiência hepática aguda grave, particularmente após a incorporação na prática clínica de uma série de estratégias baseadas em evidencias com impacto reconhecido na redução de mortalidade. Com o intuito de discutir as principais evidencias disponíveis na literatura médica sobre o assunto, a Sociedade Brasileira de Hepatologia, em conjunto com a Associação de Medicina Intensiva Brasileira e a Associação Brasileira de Transplantes de Órgãos promoveu uma reunião monotemática sobre o manejo do paciente hepatopata crítico, que ocorreu em 21 de maio de 2014 na cidade do Rio de Janeiro. O relatório da reunião foi resumido no presente manuscrito com o objetivo de nortear a prática clínica de intensivistas, gastroenterologistas e hepatologistas no manejo do paciente hepatopata em ambiente de terapia intensiva.

ABSTRACT Survival rates of critically ill patients with liver disease has sharply increased in recent years due to several improvements in the management of decompensated cirrhosis and acute liver failure. This is ascribed to the incorporation of evidence-based strategies from clinical trials aiming to reduce mortality. In order to discuss the cutting-edge evidence regarding critical care of patients with liver disease, a joint single topic conference was recently sponsored by the Brazilian Society of Hepatology in cooperation with the Brazilian Society of Intensive Care Medicine and the Brazilian Association for Organ Transplantation. This paper summarizes the proceedings of the aforementioned meeting and it is intended to guide intensive care physicians, gastroenterologists and hepatologists in the care management of patients with liver disease.

https://doi.org/10.1590/s0004-28032015000500004 34375 downloads

10.

Recomendações brasileiras de ventilação mecânica 2013. Parte 2
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Barbas, Carmen Sílvia Valente

; Ísola, Alexandre Marini

; Farias, Augusto Manoel de Carvalho

; Cavalcanti, Alexandre Biasi

; Gama, Ana Maria Casati

; Duarte, Antonio Carlos Magalhães

; Vianna, Arthur

; Serpa Neto, Ary

; Bravim, Bruno de Arruda

; Pinheiro, Bruno do Valle

; Mazza, Bruno Franco

; Carvalho, Carlos Roberto Ribeiro de

; Toufen Júnior, Carlos

; David, Cid Marcos Nascimento

; Taniguchi, Corine

; Mazza, Débora Dutra da Silveira

; Dragosavac, Desanka

; Toledo, Diogo Oliveira

; Costa, Eduardo Leite

; Caser, Eliana Bernadete

; Silva, Eliezer

; Amorim, Fabio Ferreira

; Saddy, Felipe

; Galas, Filomena Regina Barbosa Gomes

; Silva, Gisele Sampaio

; Matos, Gustavo Faissol Janot de

; Emmerich, João Claudio

; Valiatti, Jorge Luis dos Santos

; Teles, José Mario Meira

; Victorino, Josué Almeida

; Ferreira, Juliana Carvalho

; Prodomo, Luciana Passuello do Vale

; Hajjar, Ludhmila Abrahão

; Martins, Luiz Claudio

; Malbouisson, Luis Marcelo Sá

; Vargas, Mara Ambrosina de Oliveira

; Reis, Marco Antonio Soares

; Amato, Marcelo Brito Passos

; Holanda, Marcelo Alcântara

; Park, Marcelo

; Jacomelli, Marcia

; Tavares, Marcos

; Damasceno, Marta Cristina Paulette

; Assunção, Murillo Santucci César

; Damasceno, Moyzes Pinto Coelho Duarte

; Youssef, Nazah Cherif Mohamed

; Teixeira, Paulo José Zimmermann

; Caruso, Pedro

; Duarte, Péricles Almeida Delfino

; Messeder, Octavio

; Eid, Raquel Caserta

; Rodrigues, Ricardo Goulart

; Jesus, Rodrigo Francisco de

; Kairalla, Ronaldo Adib

; Justino, Sandra

; Nemer, Sergio Nogueira

; Romero, Simone Barbosa

; Amado, Verônica Moreira

Revista Brasileira de Terapia Intensiva

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sep 2014, Volume 26 N. 3 Pages 215 - 239

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

O suporte ventilatório artificial invasivo e não invasivo ao paciente grave tem evoluído e inúmeras evidências têm surgido, podendo ter impacto na melhora da sobrevida e da qualidade do atendimento oferecido nas unidades de terapia intensiva no Brasil. Isto posto, a Associação de Medicina Intensiva Brasileira (AMIB) e a Sociedade Brasileira de Pneumologia e Tisiologia (SBPT) - representadas por seu Comitê de Ventilação Mecânica e sua Comissão de Terapia Intensiva, respectivamente, decidiram revisar a literatura e preparar recomendações sobre ventilação mecânica, objetivando oferecer aos associados um documento orientador das melhores práticas da ventilação mecânica na beira do leito, com base nas evidências existentes, sobre os 29 subtemas selecionados como mais relevantes no assunto. O projeto envolveu etapas que visaram distribuir os subtemas relevantes ao assunto entre experts indicados por ambas as sociedades, que tivessem publicações recentes no assunto e/ou atividades relevantes em ensino e pesquisa no Brasil, na área de ventilação mecânica. Esses profissionais, divididos por subtemas em duplas, responsabilizaram-se por fazer uma extensa revisão da literatura mundial. Reuniram-se todos no Fórum de Ventilação Mecânica, na sede da AMIB, na cidade de São Paulo (SP), em 3 e 4 de agosto de 2013, para finalização conjunta do texto de cada subtema e apresentação, apreciação, discussão e aprovação em plenária pelos 58 participantes, permitindo a elaboração de um documento final.

https://doi.org/10.5935/0103-507X.20140034 89689 downloads

11.

Detection and cellular localization of Xanthomonas campestris pv. viticola in seeds of commercial 'Red Globe' grapes
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Tostes, Guilherme de Oliveira

; Araujo, João Sebastião de Paula

; Farias, Antônio Roberto Gomes

; Frade, Daniela Ângela Rossinol

; Olivares, Fábio Lopes

Tropical Plant Pathology

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apr 2014, Volume 39 N. 2 Pages 134 - 140

Abstract: En | Text: En | PDF: En

Commercial grapevine fruit (Vitis vinifera) of the Red Globe variety were collected in vineyards from Vale do São Francisco lower basin, an area of occurrence of grapevine bacterial canker. Seeds were extracted, classified as symptomatic or asymptomatic and processed in order to be observed under light (LM) and scanning electron microscopy (SEM) along with silver-enhanced immunogold labeling, to allow bacterial detection using a policlonal antibody against Xanthomonas campestris pv. viticola (Xcvi), etiological agent of the disease. The seed samples showed bacterial aggregates associated to the tegument surface and to the first parenchymal layer beneath the seed tegument. Bacterial identity was confirmed by immunogold labeling. This appears to be the first report of Xcvi associated to asymptomatic seeds and berries, suggesting a systemic mechanism to spread and colonize different tissues and sites, driving attention to seeds, presenting them as an important niche for survival and dissemination of this pathogen. These results point towards the need of including seed-bearing fruit in studies regarding Xcvi epidemiology.

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12.

Recomendações brasileiras de ventilação mecânica 2013. Parte I
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Barbas, Carmen Sílvia Valente

; Ísola, Alexandre Marini

; Farias, Augusto Manoel de Carvalho

; Cavalcanti, Alexandre Biasi

; Gama, Ana Maria Casati

; Duarte, Antonio Carlos Magalhães

; Vianna, Arthur

; Serpa Neto, Ary

; Bravim, Bruno de Arruda

; Pinheiro, Bruno do Valle

; Mazza, Bruno Franco

; Carvalho, Carlos Roberto Ribeiro de

; Toufen Júnior, Carlos

; David, Cid Marcos Nascimento

; Taniguchi, Corine

; Mazza, Débora Dutra da Silveira

; Dragosavac, Desanka

; Toledo, Diogo Oliveira

; Costa, Eduardo Leite

; Caser, Eliana Bernardete

; Silva, Eliezer

; Amorim, Fabio Ferreira

; Saddy, Felipe

; Galas, Filomena Regina Barbosa Gomes

; Silva, Gisele Sampaio

; Matos, Gustavo Faissol Janot de

; Emmerich, João Claudio

; Valiatti, Jorge Luis dos Santos

; Teles, José Mario Meira

; Victorino, Josué Almeida

; Ferreira, Juliana Carvalho

; Prodomo, Luciana Passuello do Vale

; Hajjar, Ludhmila Abrahão

; Martins, Luiz Cláudio

; Malbouisson, Luiz Marcelo Sá

; Vargas, Mara Ambrosina de Oliveira

; Reis, Marco Antonio Soares

; Amato, Marcelo Brito Passos

; Holanda, Marcelo Alcântara

; Park, Marcelo

; Jacomelli, Marcia

; Tavares, Marcos

; Damasceno, Marta Cristina Paulette

; Assunção, Murillo Santucci César

; Damasceno, Moyzes Pinto Coelho Duarte

; Youssef, Nazah Cherif Mohamad

; Teixeira, Paulo José Zimmermann

; Caruso, Pedro

; Duarte, Péricles Almeida Delfino

; Messeder, Octavio

; Eid, Raquel Caserta

; Rodrigues, Ricardo Goulart

; Jesus, Rodrigo Francisco de

; Kairalla, Ronaldo Adib

; Justino, Sandra

; Nemer, Sérgio Nogueira

; Romero, Simone Barbosa

; Amado, Verônica Moreira

Revista Brasileira de Terapia Intensiva

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apr 2014, Volume 26 N. 2 Pages 89 - 121

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

O suporte ventilatório artificial invasivo e não invasivo ao paciente crítico tem evoluído e inúmeras evidências têm surgido, podendo ter impacto na melhora da sobrevida e da qualidade do atendimento oferecido nas unidades de terapia intensiva no Brasil. Isto posto, a Associação de Medicina Intensiva Brasileira (AMIB) e a Sociedade Brasileira de Pneumologia e Tisiologia (SBPT) - representadas pelo seus Comitê de Ventilação Mecânica e Comissão de Terapia Intensiva, respectivamente, decidiram revisar a literatura e preparar recomendações sobre ventilação mecânica objetivando oferecer aos associados um documento orientador das melhores práticas da ventilação mecânica na beira do leito, baseado nas evidencias existentes, sobre os 29 subtemas selecionados como mais relevantes no assunto. O projeto envolveu etapas visando distribuir os subtemas relevantes ao assunto entre experts indicados por ambas as sociedades que tivessem publicações recentes no assunto e/ou atividades relevantes em ensino e pesquisa no Brasil na área de ventilação mecânica. Esses profissionais, divididos por subtemas em duplas, responsabilizaram-se por fazer revisão extensa da literatura mundial sobre cada subtema. Reuniram-se todos no Forum de Ventilação Mecânica na sede da AMIB em São Paulo, em 03 e 04 de agosto de 2013 para finalização conjunta do texto de cada subtema e apresentação, apreciação, discussão e aprovação em plenária pelos 58 participantes, permitindo a elaboração de um documento final.

Perspectives on invasive and noninvasive ventilatory support for critically ill patients are evolving, as much evidence indicates that ventilation may have positive effects on patient survival and the quality of the care provided in intensive care units in Brazil. For those reasons, the Brazilian Association of Intensive Care Medicine (Associação de Medicina Intensiva Brasileira - AMIB) and the Brazilian Thoracic Society (Sociedade Brasileira de Pneumologia e Tisiologia - SBPT), represented by the Mechanical Ventilation Committee and the Commission of Intensive Therapy, respectively, decided to review the literature and draft recommendations for mechanical ventilation with the goal of creating a document for bedside guidance as to the best practices on mechanical ventilation available to their members. The document was based on the available evidence regarding 29 subtopics selected as the most relevant for the subject of interest. The project was developed in several stages, during which the selected topics were distributed among experts recommended by both societies with recent publications on the subject of interest and/or significant teaching and research activity in the field of mechanical ventilation in Brazil. The experts were divided into pairs that were charged with performing a thorough review of the international literature on each topic. All the experts met at the Forum on Mechanical Ventilation, which was held at the headquarters of AMIB in São Paulo on August 3 and 4, 2013, to collaboratively draft the final text corresponding to each sub-topic, which was presented to, appraised, discussed and approved in a plenary session that included all 58 participants and aimed to create the final document.

https://doi.org/10.5935/0103-507X.20140017 321115 downloads

13.

Reactogenicity of yellow fever vaccines in a randomized, placebo-controlled trial
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Camacho, Luiz Antonio Bastos

; Aguiar, Savitri Gomes de

; Freire, Marcos da Silva

; Leal, Maria da Luz Fernandes

; Nascimento, Jussara Pereira do

; Iguchi, Takumi

; Lozana, José Azevedo

; Farias, Roberto Henrique Guedes

Revista de Saúde Pública

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jun 2005, Volume 39 N. 3 Pages 413 - 420

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

OBJETIVO: Comparar a reatogenicidade de três vacinas contra a febre amarela (FA) das sub-cepas WHO-17D e 17DD (diferentes lotes-semente), e placebo. MÉTODOS: Foram recrutados 1.087 adultos elegíveis para vacinação contra FA no Rio de Janeiro, RJ, Brasil. Vacinas produzidas por Bio-Manguinhos, Fiocruz (Rio de Janeiro, RJ) foram administradas ("dia zero") seguindo procedimentos adaptados para alocação randômica em blocos e "cega" para o tipo de vacina. Eventos adversos pós-vacinação foram registrados em questionários e diários preenchidos pelos participantes. Enzimas hepáticas foram medidas nos dias 0, 4-20 e 30 do estudo. A viremia foi medida nos dias 4-20. A resposta imune foi verificada em testes sorológicos nos dias 0 e 30. RESULTADOS: Os participantes eram predominantemente homens jovens. A taxa de soroconversão foi superior a 98% no grupo soronegativo antes da vacinação. Comparado ao placebo, a diferença de risco de eventos adversos locais variou de 0,9% a 2,5%, e de 3,5% a 7,4% para eventos adversos sistêmicos nos grupos vacinados. A diferença de risco desses eventos com assistência médica e/ou falta ao trabalho variou de 2,0% a 4,5%. Viremia foi detectada em 3% a 6% dos vacinados até 10 dias após a vacinação. As variações nos níveis de enzimas hepáticas pós-vacinação foram semelhantes nos grupos vacinados e placebo. CONCLUSÕES: Foi demonstrada pela primeira vez a semelhança do perfil de reatogenicidade das vacinas contra FA das cepas 17D e 17DD, comparados entre si e com placebo. As variações das enzimas hepáticas constituem evidência contra o potencial de viscerotropismo do vírus vacinal.

OBJECTIVE: To compare the reactogenicity of three yellow fever (YF) vaccines from WHO-17D and Brazilian 17DD substrains (different seed-lots) and placebo. METHODS: The study involved 1,087 adults eligible for YF vaccine in Rio de Janeiro, Brazil. Vaccines produced by Bio-Manguinhos, Fiocruz (Rio de Janeiro, Brazil) were administered ("day 0") following standardized procedures adapted to allow blinding and blocked randomization of participants to coded vaccine types. Adverse events after immunization were ascertained in an interview and in diary forms filled in by each participant. Liver enzymes were measured on days 0, 4-20 and 30 of the study. Viremia levels were measured on days 4 to 20 of follow-up. The immune response was verified through serologic tests. RESULTS: Participants were mostly young males. The seroconversion rate was above 98% among those seronegative before immunization. Compared to placebo, the excess risk of any local adverse events ranged from 0.9% to 2.5%, whereas for any systemic adverse events it ranged from 3.5% to 7.4% across vaccine groups. The excess risk of events leading to search for medical care or to interruption of work activities ranged from 2% to 4.5%. Viremia was detected in 3%-6% of vaccinees up to 10 days after vaccination. Variations in liver enzyme levels after vaccination were similar in placebo and vaccine recipients. CONCLUSIONS: The frequency of adverse events post-immunization against YF, accounting for the background occurrence of nonspecific signs and symptoms, was shown for the first time to be similar for vaccines from 17D and 17DD substrains. The data also provided evidence against viscerotropism of vaccine virus.

3015 downloads

14.

Reactogenicity of yellow fever vaccines in a randomized, placebo-controlled trial
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Camacho, Luiz Antonio Bastos

; Aguiar, Savitri Gomes de

; Freire, Marcos da Silva

; Leal, Maria da Luz Fernandes

; Nascimento, Jussara Pereira do

; Iguchi, Takumi

; Lozana, José Azevedo

; Farias, Roberto Henrique Guedes

Revista de Saúde Pública

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jun 2005, Volume 39 N. 3 Pages 413 - 420

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

1848 downloads

15.

Immunogenicity of WHO-17D and Brazilian 17DD yellow fever vaccines: a randomized trial
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Camacho, Luiz Antonio Bastos

; Freire, Marcos da Silva

; Leal, Maria da Luz Fernandes

; Aguiar, Savitri Gomes de

; Nascimento, Jussara Pereira do

; Iguchi, Takumi

; Lozana, José de Azevedo

; Farias, Roberto Henrique Guedes

Revista de Saúde Pública

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oct 2004, Volume 38 N. 5 Pages 671 - 678

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

OBJETIVO: Comparar a imunogenicidade de três vacinas contra febre amarela ) das subcepas WHO-17D e 17DD brasileira (diferentes lotes-semente). MÉTODOS: Trata-se de ensaio de equivalência envolvendo 1.087 adultos no Rio de Janeiro, RJ. As vacinas foram produzidas em Bio-Manguinhos, Fiocruz (Rio de Janeiro, Brasil) e foram administradas seguindo procedimentos adaptados para randomização em blocos, com tipos de vacinas codificados ("duplo-cego"). Anticorpos neutralizantes contra febre amarela foram dosados antes e depois da vacinação. Definiu-se equivalência como diferença nas taxas de soroconversão não superior a cinco pontos percentuais, e razão de títulos médios geométricos superior (TMG) a 0,67. RESULTADOS: As taxas de soroconversão foram iguais ou maiores do que 98% nos participantes previamente soronegativos. Na coorte completa (incluindo os previamente soropositivos) a soroconversão foi igual ou superior a 90%. As diferenças na soroconversão variaram de -0,05% a -3,02% entre os grupos de comparação. A intensidade da resposta imune também foi semelhante nos grupos: 14,5 UI/mL a 18,6 UI/mL. As razões de TMG variaram de 0,78 a 0,93. Considerando o grupo placebo, as vacinas explicaram 93% da soroconversão. Viremia foi detectada entre os dias três e sete em 2,7% dos participantes vacinados. CONCLUSÕES: A equivalência na imunogenicidade das vacinas contra a febre amarela das subcepas 17D e 17DD foi demonstrada pela primeira vez em ensaio clínico randomizado, duplo-cego, controlado com placebo. O estudo completou o processo de validação clínica do novo lote-semente de vacina, além de ampliar as bases para utilização da vacina brasileira em outros países e de trazer alternativas de subcepas para o produtor da vacina no Brasil.

OBJECTIVE: To compare the immunogenicity of three yellow fever vaccines from WHO-17D and Brazilian 17DD substrains (different seed-lots). METHODS: An equivalence trial was carried out involving 1,087 adults in Rio de Janeiro. Vaccines produced by Bio-Manguinhos, Fiocruz (Rio de Janeiro, Brazil) were administered following standardized procedures adapted to allow blocked randomized allocation of participants to coded vaccine types (double-blind). Neutralizing yellow fever antibody titters were compared in pre- and post-immunization serum samples. Equivalence was defined as a difference of no more than five percentage points in seroconversion rates, and ratio between Geometric Mean Titters (GMT) higher than 0.67. RESULTS: Seroconversion rates were 98% or higher among subjects previously seronegative, and 90% or more of the total cohort of vaccinees, including those previously seropositive. Differences in seroconversion ranged from -0.05% to -3.02%. The intensity of the immune response was also very similar across vaccines: 14.5 to 18.6 IU/mL. GMT ratios ranged from 0.78 to 0.93. Taking the placebo group into account, the vaccines explained 93% of seroconversion. Viremia was detected in 2.7% of vaccinated subjects from Day 3 to Day 7. CONCLUSIONS: The equivalent immunogenicity of yellow fever vaccines from the 17D and 17DD substrains was demonstrated for the first time in placebo-controlled double-blind randomized trial. The study completed the clinical validation process of a new vaccine seed-lot, provided evidence for use of alternative attenuated virus substrains in vaccine production for a major manufacturer, and for the utilization of the 17DD vaccine in other countries.

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