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Cowpea growth and production under water stress and soil mulching
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Souza, Tarso M. A. de

; Souto, Lauter S.

; Dutra Filho, João de A.

; Santos, Carlos S. de A.

; Silva, Luderlândio de A.

; Peixoto, Tayd D. C.

; Sá, Francisco V. da S.

Revista Brasileira de Engenharia Agrícola e Ambiental

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mar 2024, Volume 28 N. 3 elocation e271643

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO O feijão-caupi tem grande importância socioeconômica para o semiárido brasileiro, porém sofre perdas significativas de produção pelo estresse por seca, necessitando de estratégias de manejo para superar esse cenário. Nessa perspectiva, o presente estudo teve como objetivo avaliar o crescimento e a produção de genótipos de feijão-caupi sob estresse hídrico em diferentes sistemas de cultivo. O delineamento estatístico adotado foi o de blocos casualizados, em arranjo fatorial 2 × 4 × 2, correspondendo a dois genótipos de feijão-caupi (BRS Pujante e Costela de Vaca), quatro níveis de evapotranspiração da cultura (40, 60, 80 e 100%) e dois sistemas de cultivo (com e sem cobertura morta do solo). O genótipo de feijão-caupi BRS Pujante apresentou maior área foliar e peso de 100 grãos do que o genótipo Costela de Vaca em todos os níveis de evapotranspiração da cultura. Os parâmetros de produção do feijão-caupi apresentaram os melhores resultados a 80 e 100% da evapotranspiração da cultura. Independentemente do genótipo, a cobertura morta do solo proporcionou maiores valores de peso de 100 grãos de feijão-caupi. A cobertura do solo combinada com 80% da evapotranspiração da cultura é uma estratégia adequada para a produção de feijão-caupi. feijãocaupi feijão caupi brasileiro seca cenário perspectiva casualizados Vaca, , Vaca) 40, 40 (40 60 8 solo. . solo) 10 feijãocaupi. caupi. (4 6 1 (

ABSTRACT Cowpea is a crop of high socioeconomic importance for the semi-arid region of Brazil, although facing significant yield losses due to drought, requiring management strategies to overcome this scenario. From this perspective, this study aimed to evaluate the growth and yield of cowpea genotypes under drought in different cultivation systems. The statistical design was randomized blocks arranged in a 2 × 4 × 2 factorial scheme corresponding to two cowpea genotypes (BRS Pujante and Costela de Vaca), four levels of crop evapotranspiration (40, 60, 80, and 100%), and two cultivation systems (with and without soil mulching). The BRS Pujante showed a higher leaf area and 100-grain weight than Costela de Vaca under all levels of crop evapotranspiration. The cowpea yield parameters showed the best results at 80 and 100% crop evapotranspiration. Regardless of the genotype, soil mulching provided higher cowpea 100-grain weight values. Soil mulching combined with 80% crop evapotranspiration is an appropriate strategy for cowpea production. semiarid semi arid Brazil scenario perspective Vaca, , Vaca) 40, 40 (40 60 100%, 100 100%) mulching. . mulching) 100grain grain 8 genotype values production (4 6 10 ( 1

https://doi.org/10.1590/1807-1929/agriambi.v28n3e271643

Epidemiology of hydrocephalus in Brazil
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Dutra, Maurício

; da Silva, Stephanya Covas

; Beggiora, Pâmella da S.

; Santos, Marcelo V.

; Machado, Hélio R.

; Lopes, Luiza da S.

Jornal de Pediatria

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jun 2023, Volume 99 N. 3 Pages 228 - 234

Abstract: En | Text: En | PDF: En

Abstract Objective Describe the epidemiological profile and social-economic burden that hydrocephalus patients represent to the national public health system, using data available at the online database of the Brazilian Health Ministry (DataSUS). Methods This is a populational study based on descriptive statistics of all clinical and surgical appointments included in the DataSUS database. Data included herein were collected between 2015 and 2021 and subdivided into three main groups, related to hydrocephalus incidence and mortality, hospitalizations, and financial costs. Results In the study period, 3993 new cases of congenital hydrocephalus were diagnosed, with 6051 deaths overall. The mortality rate in the country was 1.5/100000 live births and the prevalence was 0.374/100000 inhabitants. The number of hospitalizations resulting from treatment procedures and complications of hydrocephalus was 137,880 and there was a reduction of up to 27.2% during the SARS-CoV-2 pandemics concerning previous years. Total costs for hydrocephalus management in the country amounted to 140,610,585.51 dollars. Conclusions Hydrocephalus has a significant impact on public health budgets and pediatric mortality rates; however, it is probably underestimated, due to the paucity of demographic data and epidemiological studies in Latin America and, specifically, in Brazil. The dataSUS also has several limitations in accessing certain data related to hydrocephalus, making it difficult to have a more assertive understanding of the disease in Brazil. The results of this study provide important guidance for future research projects in clinical and experimental hydrocephalus and also the creation of public policies for better governance and care of hydrocephalus patients. socialeconomic social economic system DataSUS. . (DataSUS) 201 202 groups period 399 diagnosed 605 overall 15100000 1 5 100000 1.5/10000 0374100000 0 374 0.374/10000 inhabitants 137880 137 880 137,88 272 27 2 27.2 SARSCoV2 SARSCoV SARS CoV SARS-CoV- years 14061058551 140 610 585 51 140,610,585.5 dollars rates however underestimated specifically Brazil (DataSUS 20 39 60 1510000 10000 1.5/1000 037410000 37 0.374/1000 13788 13 88 137,8 27. SARS-CoV 1406105855 14 61 58 140,610,585. 3 6 151000 1000 1.5/100 03741000 0.374/100 1378 8 137, 140610585 140,610,585 15100 100 1.5/10 0374100 0.374/10 14061058 140,610,58 1510 10 1.5/1 037410 0.374/1 1406105 140,610,5 151 1.5/ 03741 0.374/ 140610 140,610, 15 1.5 0374 0.374 14061 140,610 1. 037 0.37 1406 140,61 03 0.3 140,6 0. 140,

https://doi.org/10.1016/j.jped.2022.10.008

Systematic review of reverse vaccinology and immunoinformatics data for non-viral sexually transmitted infections nonviral non viral
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GOMES, LUCAS GABRIEL R.

; DUTRA, JOYCE C.F.

; PROFETA, RODRIGO

; DIAS, MARIANA V.

; GARCÍA, GLEN J.Y.

; RODRIGUES, DIEGO LUCAS N.

; GOÉS NETO, ARISTÓTELES

; ABURJAILE, FLÁVIA F.

; TIWARI, SANDEEP

; SOARES, SIOMAR C.

; AZEVEDO, VASCO

; JAISWAL, ARUN K.

Anais da Academia Brasileira de Ciências

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2023, Volume 95 Suppl. 2 elocation e20230617

Abstract: En | Text: En | PDF: En

Abstract Sexually Transmitted Infections (STIs) are a public health burden rising in developed and developing nations. The World Health Organization estimates nearly 374 million new cases of curable STIs yearly. Global efforts to control their spread have been insufficient in fulfilling their objective. As there is no vaccine for many of these infections, these efforts are focused on education and condom distribution. The development of vaccines for STIs is vital for successfully halting their spread. The field of immunoinformatics is a powerful new tool for vaccine development, allowing for the identification of vaccine candidates within a bacterium’s genome and allowing for the design of new genome-based vaccine peptides. The goal of this review was to evaluate the usage of immunoinformatics in research focused on non-viral STIs, identifying fields where research efforts are concentrated. Here we describe gaps in applying these techniques, as in the case of Treponema pallidum and Trichomonas vaginalis. (STIs nations 37 yearly objective infections distribution bacteriums bacterium s genomebased based peptides nonviral non viral concentrated techniques vaginalis 3

https://doi.org/10.1590/0001-3765202320230617

Evaluation of antimicrobial sensitivity to tetracycline exposed to non-thermal plasma nonthermal non thermal
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Cubas, Anelise L. V.

; Dutra, Ana Regina de A.

; Alves, Thiago C.

; Bianchet, Ritanara T.

; Rambo, Adriano A.

; Debacher, Nito A.

Química Nova

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2023, Volume 46 N. 3 Pages 236 - 240

Abstract: En | Text: En | PDF: En

Tetracyclines comprise a large group of broad-spectrum antibiotics used in human and veterinary medicine. Its mechanism of action, via oral ingestion, is to inhibit bacterial protein synthesis, but a large amount (70%) is eliminated without being metabolized, which presents a risk for the arising of antibiotic-resistant species. In this study, the degradation of Tetracycline TC by non-thermal plasma NTP and the antimicrobial sensitivity of the treated solution are investigated. The degradation was followed by chromatographic analysis while the efficiency of NTP in inhibiting the action of TC on bacteria was evaluated by antimicrobial sensitivity test by disk diffusion method. The results showed a high rate of TC degradation, over 90%, in 10 min of NTP treatment for a 60 mL (107 mg L-1) sample and very slow increase up to 94% in 30 min. The antimicrobial sensitivity test of TC after degradation by NTP showed no antibiotic activity. Therefore, our findings are encouraging, since the removal efficiency is below the minimum threshold require, to prevent with high probability, outgrowth of antibiotic-resistant bacteria. In addition, the results confirm that the NTP treated solution is safe to the environment. broadspectrum broad spectrum medicine ingestion synthesis 70% 70 (70% metabolized antibioticresistant resistant species study nonthermal non thermal investigated method 90 90% 1 6 107 (10 L1 L L-1 94 3 activity Therefore encouraging require probability addition environment 7 (70 9 (1 L- (7 (

https://doi.org/10.21577/0100-4042.20230006

Pre-emergent indaziflam can enhance forest seed germination in direct seeding? Preemergent Pre emergent seeding
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Dutra, F. B.

; Almeida, L. S.

; Pinto, G. C. V.

; Furlaneto, L. F.

; Souza, T. K. S.

; Viveiros, E.

; Piotrowski, I.

; Piña-Rodrigues, F. C. M.

; Silva, J. M. S.

Brazilian Journal of Biology

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2023, Volume 83 elocation e268716

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Resumo Herbicidas pré-emergentes podem contribuir para o controle da competição de plantas daninhas em restauração por semeadura direta, porém é necessário entender seus efeitos em sementes de florestais nativas. O objetivo do estudo foi avaliar o potencial impacto do herbicida indaziflam na germinação de 17 espécies nativas de florestas tropicais. Para isso, uma dosagem ligeiramente acima da recomendada (180 mL do produto em 200 L de água) foi comparada com a testemunha sem herbicida. O grau de sensibilidade de cada espécie, foi calculado pela razão entre a porcentagem de germinação com herbicida (GH) e a testemunha sem herbicida (GC) e classificando-as como: extremamente sensível (ES= (GH/GC) <0,25), sensível (S=0,25< (GH/GC) <0,50), baixa sensibilidade (LS=0,50< (GH/GC) <0,75), indiferente - I=0,75< (GH/GC) <1,0) e potencializado (P= (GH/GC) >1). O herbicida promoveu redução significativa na média de germinação em mais de 35% (n=6) das espécies avaliadas e 59% (n=10) foram sensíveis ou extremamente sensíveis ao indaziflam e apenas três espécies não germinaram. Por outro lado, 29,4% (n=5) apresentaram baixa sensibilidade ou indiferença ao herbicida, enquanto a germinação das sementes foi levemente aumentada pelo indaziflam para 11,7% (n=2). O indaziflam pré-emergente pode ser recomendado na restauração de semeadura direta pois apenas 17,6% (n=3). O indaziflam pré-emergente pode ser recomendado na restauração de semeadura direta pois apenas 17,6% (n=3) das espécies foram inibidas pelo pré-emergente. No entanto, o efeito do indaziflam variou em função da espécie e requer mais estudos para apoiar o uso em larga escala na semeadura direta. préemergentes pré emergentes 1 tropicais isso 180 (18 20 água GH (GH GC (GC classificandoas classificando as como ES= ES (ES GH/GC GHGC (GH/GC <0,25, 025 <0,25 , 0 25 <0,25) S=0,25< S025 S (S=0,25 <0,50, 050 <0,50 50 <0,50) LS=0,50< LS050 LS (LS=0,50 <0,75, 075 <0,75 75 <0,75) I075 I I=0,75 <1,0 10 P= P (P >1. >1 . >1) 35 n=6 n6 n 6 (n=6 59 n=10 n10 (n=10 germinaram lado 294 29 4 29,4 n=5 n5 5 (n=5 117 11 7 11,7 n=2. n2 n=2 2 (n=2) préemergente emergente 176 17,6 n=3. n3 n=3 3 (n=3 préemergente. emergente. entanto 18 (1 02 <0,2 S=0,25 S02 (S=0,2 05 <0,5 LS=0,50 LS05 (LS=0,5 07 <0,7 I07 I=0,7 <1, > n= (n= n=1 n1 (n=1 29, 11, (n=2 17, ( <0, S=0,2 S0 (S=0, LS=0,5 LS0 (LS=0, I0 I=0, <1 (n <0 S=0, (S=0 LS=0, (LS=0 I=0 < S=0 (S= LS=0 (LS= I= S= (S LS= (LS

Abstract Pre-emergent herbicides can contribute to the control of weed competition in direct seeding restoration, however it is necessary to evaluate their effects on seeds of native tropical forest species. The aim of the study was to assess the potential impact of the herbicide indaziflam on the germination of 17 forest species. For this, a dosage of 180 mL of the product in 200L of water was compared to the control without herbicide. The degree of sensitivity of each species was calculated by a ratio between the percentage of germination with herbicide (GH) and the control without herbicide (GC) classifying them as: extremely sensitive (ES= (GH/GC) <0.25), sensitive (S=0.25< (GH/GC) <0.50), low sensitivity (LS=0.50< (GH/GC) <0.75), indifferent (I=0.75< (GH/GC) <1.0) and potentiated (P= (GH/GC) >1). The herbicide promoted a significant reduction in mean germination in 35% (n=6) of the species and 59% (n = 10) were sensitive or extremely sensitive to indaziflam, and only three did not germinate. On the other hand, 29.4% (n=5) showed low sensitivity or indifference to the herbicide, while seed germination was slightly increased by indaziflam to 11.7% (n=2). Pre-emergent indaziflam can be recommended in direct seeding restoration, as only 17.6% (n=3) of the species were inhibited by pre-emergent. However, the effect of indaziflam varies by species and requires further studies to support large-scale use in direct seeding. Preemergent Pre emergent restoration 1 this 18 L GH (GH GC (GC ES= ES (ES GH/GC GHGC (GH/GC <0.25, 025 <0.25 , 0 25 <0.25) S=0.25< S025 S (S=0.25 <0.50, 050 <0.50 50 <0.50) LS=0.50< LS050 LS (LS=0.50 <0.75, 075 <0.75 75 <0.75) I=0.75< I075 I (I=0.75 <1.0 10 P= P (P >1. >1 . >1) 35 n=6 n6 n 6 (n=6 59 germinate hand 294 29 4 29.4 n=5 n5 5 (n=5 117 11 7 11.7 n=2. n2 n=2 2 (n=2) 176 17.6 n=3 n3 3 (n=3 preemergent. preemergent pre emergent. pre-emergent However largescale large scale 02 <0.2 S=0.25 S02 (S=0.2 05 <0.5 LS=0.50 LS05 (LS=0.5 07 <0.7 I=0.75 I07 (I=0.7 <1. > n= (n= 29. 11. (n=2 17. <0. S=0.2 S0 (S=0. LS=0.5 LS0 (LS=0. I=0.7 I0 (I=0. <1 <0 S=0. (S=0 LS=0. (LS=0 I=0. (I=0 < S=0 (S= LS=0 (LS= I=0 (I= S= (S LS= (LS I= (I

https://doi.org/10.1590/1519-6984.268716

[SciELO Preprints] - Guideline of the Brazilian Society of Cardiology on Diagnosis and Treatment of Patients with Chagas Disease Cardiomyopathy
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Marin-Neto, José Antonio Rassi Jr., Anis Moraes Oliveira, Gláucia M. Lemos Correia, Luís Claudio Novaes Ramos Jr., Alberto Hasslocher-Moreno, Alejandro Marcel Luquetti Ostermayer, Alejandro Sousa, Andréa Silvestre de Amato Vincenzo de Paola, Angelo Sobral de Sousa, Antonio Carlos Pinho Ribeiro, Antonio Luiz Correia Filho, Dalmo Moraes de Souza, Dilma do Socorro Cunha-Neto, Edecio J. A. Ramires, Felix Bacal, Fernando Pereira Nunes, Maria do Carmo Martinelli Filho, Martino Ibrahim Scanavacca, Maurício Magalhães Saraiva, Roberto Alves de Oliveira Júnior, Wilson M. Lorga-Filho, Adalberto de Jesus Benevides de Almeida Guimarães, Adriana Lopes Latado Braga, Adriana Sarmento de Oliveira, Adriana V. L. Sarabanda, Alvaro Yecê das Neves Pinto, Ana Assis Lopes do Carmo, André Schmidt, André Costa, Andréa Rodrigues da Ianni, Barbara Maria Markman Filho, Brivaldo Eduardo Rochitte, Carlos Thé Macedo, Carolina Mady, Charles Chevillard, Christophe Bittencourt das Virgens, Cláudio Marcelo Nery de Castro, Cleudson De Paoli de Carvalho Britto, Constança Felícia Pisani, Cristiano do Carmo Rassi, Daniela C. Sobral Filho, Dario Rodrigues Almeida, Dirceu A. Bocchi, Edimar T. Mesquita, Evandro de Souza Nogueira Sardinha Mendes, Fernanda Pereira, Francisca Tatiana Sperandio da Silva, Gilberto Marcelo de Lima Peixoto, Giselle Glotz de Lima, Gustavo H. Veloso, Henrique Turin Moreira, Henrique Bellotti Lopes, Hugo Masciarelli Francisco Pinto, Ibraim Pinto Dias, João Carlos Bemfica, João Marcos Silva-Nunes, João Paulo Soares Barreto-Filho, José Augusto Kerr Saraiva, José Francisco Lannes-Vieira, Joseli Menezes Oliveira, Joselina Luzia V. Armaganijan, Luciana Martins, Luiz Cláudio C. Sangenis, Luiz Henrique Barbosa, Marco Paulo Almeida-Santos, Marcos Antônio Simões, Marcos Vinicius Shikanai-Yasuda, Maria Aparecida Vieira Moreira, Maria da Consolação Higuchi, Maria de Lourdes Costa Monteiro, Maria Rita de Cássia Felix Mediano, Mauro Felippe Maia Lima, Mayara T. Oliveira, Maykon Moreira Dias Romano , Minna Nitz, Nadjar de Tarso Jorge Medeiros, Paulo Vieira Alves, Renato Alkmim Teixeira, Ricardo Coury Pedrosa, Roberto Aras, Roque Morais Torres, Rosália dos Santos Povoa, Rui Manoel Rassi, Sérgio Gabriel Salles Xavier, Sérgio Marinho Martins Alves , Silvia B. N. Tavares, Suelene Lima Palmeira, Swamy da Silva Junior, Telêmaco Luiz da Rocha Rodrigues, Thiago Madrini Junior, Vagner Maia da Costa , Veruska Dutra, Walderez

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Publication date: 2022-10-11

Abstract: En Pt

This guideline aimed to update the concepts and formulate the standards of conduct and scientific evidence that support them, regarding the diagnosis and treatment of the Cardiomyopathy of Chagas disease, with special emphasis on the rationality base that supported it.nbsp; Chagas disease in the 21st century maintains an epidemiological pattern of endemicity in 21 Latin American countries. Researchers and managers from endemic and non-endemic countries point to the need to adopt comprehensive public health policies to effectively control the interhuman transmission of T. cruzi infection, and to obtain an optimized level of care for already infected individuals, focusing on diagnostic and therapeutic opportunistic opportunities. nbsp; Pathogenic and pathophysiological mechanisms of the Cardiomyopathy of Chagas disease were revisited after in-depth updating and the notion that necrosis and fibrosis are stimulated by tissue parasitic persistence and adverse immune reaction, as fundamental mechanisms, assisted by autonomic and microvascular disorders, was well established. Some of them have recently formed potential targets of therapies.nbsp; The natural history of the acute and chronic phases was reviewed, with enhancement for oral transmission, indeterminate form and chronic syndromes. Recent meta-analyses of observational studies have estimated the risk of evolution from acute and indeterminate forms and mortality after chronic cardiomyopathy. Therapeutic approaches applicable to individuals with Indeterminate form of Chagas disease were specifically addressed. All methods to detect structural and/or functional alterations with various cardiac imaging techniques were also reviewed, with recommendations for use in various clinical scenarios. Mortality risk stratification based on the Rassi score, with recent studies of its application, was complemented by methods that detect myocardial fibrosis.nbsp; The current methodology for etiological diagnosis and the consequent implications of trypanonomic treatment deserved a comprehensive and in-depth approach. Also the treatment of patients at risk or with heart failure, arrhythmias and thromboembolic events, based on pharmacological and complementary resources, received special attention. Additional chapters supported the conducts applicable to several special contexts, including t. cruzi/HIV co-infection, risk during surgeries, in pregnant women, in the reactivation of infection after heart transplantation, and others.nbsp; nbsp;nbsp; Finally, two chapters of great social significance, addressing the structuring of specialized services to care for individuals with the Cardiomyopathy of Chagas disease, and reviewing the concepts of severe heart disease and its medical-labor implications completed this guideline.

Esta diretriz teve como objetivo principal atualizar os conceitos e formular as normas de conduta e evidências científicas que as suportam, quanto ao diagnóstico e tratamento da CDC, com especial ênfase na base de racionalidade que a embasou. A DC no século XXI mantém padrão epidemiológico de endemicidade em 21 países da América Latina. Investigadores e gestores de países endêmicos e não endêmicos indigitam a necessidade de se adotarem políticas abrangentes, de saúde pública, para controle eficaz da transmissão inter-humanos da infecção pelo T. cruzi, e obter-se nível otimizado de atendimento aos indivíduos já infectados, com foco em oportunização diagnóstica e terapêutica. Mecanismos patogênicos e fisiopatológicos da CDC foram revisitados após atualização aprofundada e ficou bem consolidada a noção de que necrose e fibrose sejam estimuladas pela persistência parasitária tissular e reação imune adversa, como mecanismos fundamentais, coadjuvados por distúrbios autonômicos e microvasculares. Alguns deles recentemente constituíram alvos potenciais de terapêuticas. A história natural das fases aguda e crônica foi revista, com realce para a transmissão oral, a forma indeterminada e as síndromes crônicas. Metanálises recentes de estudos observacionais estimaram o risco de evolução a partir das formas aguda e indeterminada e de mortalidade após instalação da cardiomiopatia crônica. Condutas terapêuticas aplicáveis aos indivíduos com a FIDC foram abordadas especificamente. Todos os métodos para detectar alterações estruturais e/ou funcionais com variadas técnicas de imageamento cardíaco também foram revisados, com recomendações de uso nos vários cenários clínicos. Estratificação de risco de mortalidade fundamentada no escore de Rassi, com estudos recentes de sua aplicação, foi complementada por métodos que detectam fibrose miocárdica. A metodologia atual para diagnóstico etiológico e as consequentes implicações do tratamento tripanossomicida mereceram enfoque abrangente e aprofundado. Também o tratamento de pacientes em risco ou com insuficiência cardíaca, arritmias e eventos tromboembólicos, baseado em recursos farmacológicos e complementares, recebeu especial atenção. Capítulos suplementares subsidiaram as condutas aplicáveis a diversos contextos especiais, entre eles o da co-infecção por T. cruzi/HIV, risco durante cirurgias, em grávidas, na reativação da infecção após transplante cardíacos, e outros.nbsp;nbsp;nbsp; Por fim, dois capítulos de grande significado social, abordando a estruturação de serviços especializados para atendimento aos indivíduos com a CDC, e revisando os conceitos de cardiopatia grave e suas implicações médico-trabalhistas completaram esta diretriz.nbsp;

https://doi.org/10.1590/SciELOPreprints.4820

Multivariate classification of cotton cultivars tolerant to salt stress
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Marcelino, Aline D. A. de L.

; Fernandes, Pedro D.

; Ramos, Jean P. C.

; Dutra, Wellison F.

; Cavalcanti, José J. V.

; Santos, Roseane C. dos

Revista Brasileira de Engenharia Agrícola e Ambiental

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apr 2022, Volume 26 N. 4 Pages 266 - 273

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO Dois métodos multivariados foram adotados para classificar genótipos de algodoeiro tolerantes ao sal com base no crescimento e nas características fisiológicas. Os genótipos foram cultivados em casa de vegetação e submetidos a 45 dias de irrigação com água salina, a partir da fase V4. A irrigação foi feita com água salina com CEa de 6,0 dS m-1. O delineamento experimental foi em blocos casualizados com fatorial, sendo nove cultivares, dois tratamentos (controle: 0,6 e 6,0 dS m-1) e quatro repetições. As plantas foram avaliadas quanto a variáveis de crescimento e fisiológicas. Os dados foram analisados estatisticamente por meio de analises univariada e multivariada. Nesse último, os modelos não hierárquicos (componentes principais, CP) e hierárquico (UPGMA) foram usados para classificação das cultivares. Diferenças significativas foram encontradas entre as cultivares com base nas análises univariadas. As variáveis que diferiram estatisticamente foram usadas para as análises multivariadas. Quatro grupos foram identificados com a mesma composição nos métodos PC e UPGMA. Entre eles, um conteve as cultivares BRS Seridó, BRS 286, FMT 705 e BRS Rubi, que se mostraram tolerantes ao estresse salino imposto as plantas. Os dados de fotossíntese, transpiração e condutância estomática foram os mais contributivos para classificação das cultivares, pelo método das componentes principais.

ABSTRACT Two multivariate methods were adopted to classify salt-tolerant cotton genotypes based on their growth and physiological traits. The genotypes were cultivated in a greenhouse and subjected to 45 days of irrigation with saline water from the V4 phase onwards. Irrigation was performed with saline water with electrical conductivity (ECw) of 6.0 dS m-1. A factorial-randomized block design was adopted with nine cultivars, two treatments of ECw (0.6 as the control, and 6.0 dS m-1), and four replicates. Plants were evaluated for growth, gas exchange, and photosynthesis. The data were statistically analyzed using univariate and multivariate methods. For the latter, non-hierarchical (principal component, PC) and hierarchical (UPGMA) models were used for the classification of cultivars. Significant differences were found between cultivars based on univariate analyses, and the traits that differed statistically were used for multivariate analyses. Four groups were identified with the same composition in both the PC and UPGMA methods. Among them, one contained the cultivars BRS Seridó, BRS 286, FMT 705, and BRS Rubi, which were tolerant to salt stress imposed on the plants. Photosynthesis, transpiration, and stomatal conductance data were the main contributors to the classification of cultivars using the principal component method.

https://doi.org/10.1590/1807-1929/agriambi.v26n4p266-273

Thanatosis behavior during oviposition in Tropidurus itambere Rodrigues, 1987
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Lipinski, V.

; Souza, B. A.

; Corrêa, F.

; Dutra, E. S.

Brazilian Journal of Biology

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2022, Volume 82 elocation e233930

Abstract: En | Text: En | PDF: En

https://doi.org/10.1590/1519-6984.233930 127 downloads

EXEMPLIFICANDO O USO DO MACRO MAPEAMENTO ELEMENTAR POR XRF (MA-XRF) NA INVESTIGAÇÃO FORENSE DE OBRAS DE ARTE
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Pimenta, André R.

; Felix, Valter S.

; Silva, Daniele M.

; Pereira, Marcelo O.

; Oliveira, Ana L.

; Ferreira, Douglas S.

; Carvalho, Cristiano S.

; Silva, Fabricio L.

; Gonçalves, Elicardo A.S.

; Dutra, Rafael S.

; Pereira, Leandro O.

; Pereira, Ronaldo V.

; Freitas, Renato P.

Química Nova

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2022, Volume 45 N. 3 Pages 297 - 303

Abstract: Pt | Text: Pt | PDF: Pt

EXEMPLIFYING THE USE OF MACRO ELEMENTAL MAPPING BY XRF (MA-XRF) SCANNING IN FORENSIC INVESTIGATION OF ARTWORKS. This work exemplifies the potential of employing macro elemental mapping by XRF (MA XRF) scanning in the forensic investigation of artworks. The results of the investigation of two artworks of different styles and periods are presented and discussed. The images provided by MA-XRF scanning have information about the elemental distribution, which is related to pigments used in the artwork. These images bring different information such as: if there are underlying paintings; if a given pigment was used for the purpose of restoration or in the creation. Therefore, through the images, it is possible to obtain information, which refers to the process of creating the artwork and the history of conservation and restoration. Through the results, it was possible to verify that the two artworks are probably false because they present anachronistic pigments with the supposed period of creation. The analyzes were performed using a portable MA-XRF scanning system, and in addition to presenting the utility of the instrument in these investigations, the work also presents possibilities to explore the MA-XRF data through multivariate statistical analysis and image correlation.

https://doi.org/10.21577/0100-4042.20170826

10.

Práticas de ressuscitação volêmica em unidades de terapia intensiva brasileiras: uma análise secundária do estudo Fluid-TRIPS
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Freitas, Flavio Geraldo Rezende de

; Hammond, Naomi

; Li, Yang

; Azevedo, Luciano Cesar Pontes de

; Cavalcanti, Alexandre Biasi

; Taniguchi, Leandro

; Gobatto, André

; Japiassú, André Miguel

; Bafi, Antonio Tonete

; Mazza, Bruno Franco

; Noritomi, Danilo Teixeira

; Dal-Pizzol, Felipe

; Bozza, Fernando

; Salluh, Jorge Ibrahin Figueira

; Westphal, Glauco Adrieno

; Soares, Márcio

; Assunção, Murillo Santucci César de

; Lisboa, Thiago

; Lobo, Suzana Margarete Ajeje

; Barbosa, Achilles Rohlfs

; Ventura, Adriana Fonseca

; Souza, Ailson Faria de

; Silva, Alexandre Francisco

; Toledo, Alexandre

; Reis, Aline

; Cembranel, Allan

; Rea Neto, Alvaro

; Gut, Ana Lúcia

; Justo, Ana Patricia Pierre

; Santos, Ana Paula

; Albuquerque, André Campos D. de

; Scazufka, André

; Rodrigues, Antonio Babo

; Fernandino, Bruno Bonaccorsi

; Silva, Bruno Goncalves

; Vidal, Bruno Sarno

; Pinheiro, Bruno Valle

; Pinto, Bruno Vilela Costa

; Feijo, Carlos Augusto Ramos

; Abreu Filho, Carlos de

; Bosso, Carlos Eduardo da Costa Nunes

; Moreira, Carlos Eduardo Nassif

; Ramos, Carlos Henrique Ferreira

; Tavares, Carmen

; Arantes, Cidamaiá

; Grion, Cintia

; Mendes, Ciro Leite

; Kmohan, Claudio

; Piras, Claudio

; Castro, Cristine Pilati Pileggi

; Lins, Cyntia

; Beraldo, Daniel

; Fontes, Daniel

; Boni, Daniela

; Castiglioni, Débora

; Paisani, Denise de Moraes

; Pedroso, Durval Ferreira Fonseca

; Mattos, Ederson Roberto

; Brito Sobrinho, Edgar de

; Troncoso, Edgar M. V.

; Rodrigues Filho, Edison Moraes

; Nogueira, Eduardo Enrico Ferrari

; Ferreira, Eduardo Leme

; Pacheco, Eduardo Souza

; Jodar, Euzebio

; Ferreira, Evandro L. A.

; Araujo, Fabiana Fernandes de

; Trevisol, Fabiana Schuelter

; Amorim, Fábio Ferreira

; Giannini, Fabio Poianas

; Santos, Fabrício Primitivo Matos

; Buarque, Fátima

; Lima, Felipe Gallego

; Costa, Fernando Antonio Alvares da

; Sad, Fernando Cesar dos Anjos

; Aranha, Fernando G.

; Ganem, Fernando

; Callil, Flavio

; Costa Filho, Francisco Flávio

; Dall´Arto, Frederico Toledo Campo

; Moreno, Geovani

; Friedman, Gilberto

; Moralez, Giulliana Martines

; Silva, Guilherme Abdalla da

; Costa, Guilherme

; Cavalcanti, Guilherme Silva

; Betônico, Gustavo Navarro

; Reis, Hélder

; Araujo, Helia Beatriz N.

; Hortiz Júnior, Helio Anjos

; Guimaraes, Helio Penna

; Urbano, Hugo

; Maia, Israel

; Santiago Filho, Ivan Lopes

; Farhat Júnior, Jamil

; Alvarez, Janu Rangel

; Passos, Joel Tavares

; Paranhos, Jorge Eduardo da Rocha

; Marques, José Aurelio

; Moreira Filho, José Gonçalves

; Andrade, Jose Neto

; Sobrinho, José Onofre de C

; Bezerra, Jose Terceiro de Paiva

; Alves, Juliana Apolônio

; Ferreira, Juliana

; Gomes, Jussara

; Sato, Karina Midori

; Gerent, Karine

; Teixeira, Kathia Margarida Costa

; Conde, Katia Aparecida Pessoa

; Martins, Laércia Ferreira

; Figueirêdo, Lanese

; Rezegue, Leila

; Tcherniacovsk, Leonardo

; Ferraz, Leone Oliveira

; Cavalcante, Liane

; Rabelo, Ligia

; Miilher, Lilian

; Garcia, Lisiane

; Tannous, Luana

; Hajjar, Ludhmila Abrahão

; Paciência, Luís Eduardo Miranda

; Cruz Neto, Luiz Monteiro da

; Bley, Macia Valeria

; Sousa, Marcelo Ferreira

; Puga, Marcelo Lourencini

; Romano, Marcelo Luz Pereira

; Nobrega, Marciano

; Arbex, Marcio

; Rodrigues, Márcio Leite

; Guerreiro, Márcio Osório

; Rocha, Marcone

; Alves, Maria Angela Pangoni

; Rosa, Maria Doroti

; Dias, Mariza D’Agostino

; Martins, Miquéias

; Oliveira, Mirella de

; Moretti, Miriane Melo Silveira

; Matsui, Mirna

; Messender, Octavio

; Santarém, Orlando Luís de Andrade

; Silveira, Patricio Júnior Henrique da

; Vassallo, Paula Frizera

; Antoniazzi, Paulo

; Gottardo, Paulo César

; Correia, Paulo

; Ferreira, Paulo

; Torres, Paulo

; Silva, Pedro Gabrile M. de Barros e

; Foernges, Rafael

; Gomes, Rafael

; Moraes, Rafael

; Nonato filho, Raimundo

; Borba, Renato Luis

; Gomes, Renato V

; Cordioli, Ricardo

; Lima, Ricardo

; López, Ricardo Pérez

; Gargioni, Ricardo Rath de Oliveira

; Rosenblat, Richard

; Souza, Roberta Machado de

; Almeida, Roberto

; Narciso, Roberto Camargo

; Marco, Roberto

; waltrick, Roberto

; Biondi, Rodrigo

; Figueiredo, Rodrigo

; Dutra, Rodrigo Santana

; Batista, Roseane

; Felipe, Rouge

; Franco, Rubens Sergio da Silva

; Houly, Sandra

; Faria, Sara Socorro

; Pinto, Sergio Felix

; Luzzi, Sergio

; Sant’ana, Sergio

; Fernandes, Sergio Sonego

; Yamada, Sérgio

; Zajac, Sérgio

; Vaz, Sidiner Mesquita

; Bezerra, Silvia Aparecida Bezerra

; Farhat, Tatiana Bueno Tardivo

; Santos, Thiago Martins

; Smith, Tiago

; Silva, Ulysses V. A.

; Damasceno, Valnei Bento

; Nobre, Vandack

; Dantas, Vicente Cés de Souza

; Irineu, Vivian Menezes

; Bogado, Viviane

; Nedel, Wagner

; Campos Filho, Walther

; Dantas, Weidson

; Viana, William

; Oliveira Filho, Wilson de

; Delgadinho, Wilson Martins

; Finfer, Simon

; Machado, Flavia Ribeiro

Revista Brasileira de Terapia Intensiva

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apr 2021, Volume 33 N. 2 Pages 206 - 218

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO Objetivo: Descrever as práticas de ressuscitação volêmica em unidades de terapia intensiva brasileiras e compará-las com as de outros países participantes do estudo Fluid-TRIPS. Métodos: Este foi um estudo observacional transversal, prospectivo e internacional, de uma amostra de conveniência de unidades de terapia intensiva de 27 países (inclusive o Brasil), com utilização da base de dados Fluid-TRIPS compilada em 2014. Descrevemos os padrões de ressuscitação volêmica utilizados no Brasil em comparação com os de outros países e identificamos os fatores associados com a escolha dos fluidos. Resultados: No dia do estudo, foram incluídos 3.214 pacientes do Brasil e 3.493 pacientes de outros países, dos quais, respectivamente, 16,1% e 26,8% (p < 0,001) receberam fluidos. A principal indicação para ressuscitação volêmica foi comprometimento da perfusão e/ou baixo débito cardíaco (Brasil 71,7% versus outros países 56,4%; p < 0,001). No Brasil, a percentagem de pacientes que receberam soluções cristaloides foi mais elevada (97,7% versus 76,8%; p < 0,001), e solução de cloreto de sódio a 0,9% foi o cristaloide mais comumente utilizado (62,5% versus 27,1%; p < 0,001). A análise multivariada sugeriu que os níveis de albumina se associaram com o uso tanto de cristaloides quanto de coloides, enquanto o tipo de prescritor dos fluidos se associou apenas com o uso de cristaloides. Conclusão: Nossos resultados sugerem que cristaloides são usados mais frequentemente do que coloides para ressuscitação no Brasil, e essa discrepância, em termos de frequências, é mais elevada do que em outros países. A solução de cloreto de sódio 0,9% foi o cristaloide mais frequentemente prescrito. Os níveis de albumina sérica e o tipo de prescritor de fluidos foram os fatores associados com a escolha de cristaloides ou coloides para a prescrição de fluidos.

Abstract Objective: To describe fluid resuscitation practices in Brazilian intensive care units and to compare them with those of other countries participating in the Fluid-TRIPS. Methods: This was a prospective, international, cross-sectional, observational study in a convenience sample of intensive care units in 27 countries (including Brazil) using the Fluid-TRIPS database compiled in 2014. We described the patterns of fluid resuscitation use in Brazil compared with those in other countries and identified the factors associated with fluid choice. Results: On the study day, 3,214 patients in Brazil and 3,493 patients in other countries were included, of whom 16.1% and 26.8% (p < 0.001) received fluids, respectively. The main indication for fluid resuscitation was impaired perfusion and/or low cardiac output (Brazil: 71.7% versus other countries: 56.4%, p < 0.001). In Brazil, the percentage of patients receiving crystalloid solutions was higher (97.7% versus 76.8%, p < 0.001), and 0.9% sodium chloride was the most commonly used crystalloid (62.5% versus 27.1%, p < 0.001). The multivariable analysis suggested that the albumin levels were associated with the use of both crystalloids and colloids, whereas the type of fluid prescriber was associated with crystalloid use only. Conclusion: Our results suggest that crystalloids are more frequently used than colloids for fluid resuscitation in Brazil, and this discrepancy in frequencies is higher than that in other countries. Sodium chloride (0.9%) was the crystalloid most commonly prescribed. Serum albumin levels and the type of fluid prescriber were the factors associated with the choice of crystalloids or colloids for fluid resuscitation.

https://doi.org/10.5935/0103-507x.20210028 273 downloads

11.

Investigação experimental da lei de Stokes em discos: uma abordagem de baixo custo por vídeo-análise de oscilações amortecidas
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Pereira, L. O.

; Ferreira, D. S. R.

; Freitas, R. P.

; Pimenta, A. R.

; Felix, V. S.

; Gonçalves, E. A. S.

; Dutra, R. S.

Revista Brasileira de Ensino de Física

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2021, Volume 43 elocation e20200498

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Neste trabalho apresentamos uma atividade de baixo custo que visa a investigação da força de arrasto, que atua na geometria de um disco, através das oscilações amortecidas de um sistema massa-mola. Utilizando discos de papelão de diferentes raios e controlando a massa do sistema oscilatório, mantendo-a fixa, estudamos a influência da variação exclusiva da magnitude da força de arrasto na dinâmica desse sistema, por meio da variação do raio do disco, mantendo todos os outros parâmetros acessíveis do sistema fixos. A técnica de vídeo-análise é utilizada para rastrear o movimento do sistema, levando o estudante a investigar e a extrair conclusões acerca da dependência funcional da força de atrito viscosa com o raio do disco, bem como a obter a constante elástica da mola e uma estimativa para a espessura dos discos.

In this work we present a low-cost activity that aims to investigate the drag force, which acts on the geometry of a disc, through the damped oscillations of a mass-spring system. Using cardboard disks of different radii and controlling the mass of the oscillatory system, keeping it fixed, we studied the influence of the exclusive variation of the magnitude of the drag force on the dynamics of this system, by varying the radius of the disk, keeping all the others fixed system accessible parameters. The video analysis technique is used to track the movement of the system, leading the student to investigate and draw conclusions about the functional dependence of the viscous frictional force with the radius of the disk, as well as to obtain the spring elastic constant and an estimate for the thickness of the disks.

https://doi.org/10.1590/1806-9126-rbef-2020-0498 921 downloads

12.

Diversity of the diterpenes in the leaves of Xylopia laevigata (Annonaceae) and their cytotoxicities Annonaceae (Annonaceae
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Costa, Emmanoel V.

; Sampaio, Marília F. C.

; Menezes, Leociley R. A.

; Dutra, Lívia M.

; Costa, Cinara O. S.

; Pinheiro, Maria Lúcia B.

; Silva, Felipe M. A. da

; Soares, Milena B. P.

; Bezerra, Daniel P.

; Barison, Andersson

; Koolen, Hector H. F.

Química Nova

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apr 2020, Volume 43 N. 4 Pages 419 - 425

Abstract: En | Text: En | PDF: En

The phytochemical investigation of the hexane extract which was obtained from the leaves of Xylopia laevigata (Annonaceae) produced six terpenes. Among these, five diterpenes named abieta-7,13-dien-3-one, ent-7β-acetoxy-16β-hydroxy-kaurane, 4-epi-cupressic acid, powerol, and labdorffianic acid B, and one oxygenated sesquiterpene, spathulenol. This is the first time these isolated diterpenes from X. laevigata have been described. The different structures belong to the abietane, kaurane, and labdane diterpenoids, and are indicative of the chemical diversity found in X. laevigata. Moreover, the diterpene ent-7β-acetoxy-16β-hydroxy-kaurane is reported herein for the first time as a natural product, and 4-epi-cupressic acid, powerol, and labdorffianic acid B are reported for the first time in the Annonaceae family. The structures of the isolated compounds were established by extensive analyses using 1D and 2D NMR spectroscopy in combination with MS. The cytotoxic activities of compounds abieta-7,13-dien-3-one, spathulenol, 4-epi-cupressic acid, and powerol were evaluated against tumor and non-tumor cell lines, in which spathulenol was found to be the most active, mainly against K562 with an IC50 value of 17.20 μmol L-1. (Annonaceae terpenes abieta7,13dien3one, abieta713dien3one abietadienone abieta 7,13 dien 3 one, 7 13 abieta-7,13-dien-3-one ent7βacetoxy16βhydroxykaurane, ent7βacetoxy16βhydroxykaurane entβacetoxyβhydroxykaurane ent 7β acetoxy 16β hydroxy kaurane β 4epicupressic epicupressic 4 epi cupressic sesquiterpene X described abietane diterpenoids Moreover product family D MS nontumor non lines active K K56 IC IC5 1720 17 20 17.2 L1. L1 L 1. 1 L-1 abieta7 13dien3one abieta7,13dien3one 713 7,1 βacetoxy βhydroxykaurane K5 172 2 17. L- dienone 71 7,

https://doi.org/10.21577/0100-4042.20170504 335 downloads

13.

Rapid assessment of the ichthyofauna of the southern Guiana Shield tributaries of the Amazonas River in Pará, Brazil
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DUTRA, Guilherme M.

; FREITAS, Tiago M. S.

; PRUDENTE, Bruno S.

; SALVADOR, Gilberto N.

; LEÃO, Manuela D. V.

; PEIXOTO, Luiz A. W.

; MENDONÇA, Marina B.

; NETTO-FERREIRA, André L.

; SILVA, Fabio R.

; MONTAG, Luciano F. A.

; WOSIACKI, Wolmar B.

Acta Amazonica

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mar 2020, Volume 50 N. 1 Pages 24 - 36

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO O Sistema de Drenagens do Norte do Pará abrange os afluentes da margem esquerda do Rio Amazonas, na região sul do Escudo das Guianas no estado do Pará, Brasil. Cinco das áreas estaduais protegidas da região são consideradas estratégicas para a conservação da biodiversidade. No presente estudo, nós acessamos a ictiofauna das cinco áreas de proteção estadual do Sistema de Drenagens do Norte do Pará. Sete expedições foram realizadas entre os meses de janeiro de 2008 e janeiro de 2009, amostrando trechos das bacias dos rios Cuminá, Cuminapanema, Curuá, Jari, Mapuera, Nhamundá e Paru. As expedições resultaram em 286 espécies pertencentes a 38 famílias e oito ordens, incluindo sete novos registros de peixes para o Brasil, seis deles também representando novos registros para a bacia Amazônica. Nossos resultados fornecem uma base sólida para futuros programas de pesquisa e conservação nas áreas de proteção da região.

ABSTRACT The Northern Pará Drainage System encompasses the left-bank tributaries of the Amazonas River in the southern Guiana Shield region of Pará state, Brazil. Five of the region’s state protected areas are considered strategic for the conservation of its biodiversity. In the present study, we assessed the ichthyofauna of the five state protected areas of the Northern Pará Drainage System. Seven expeditions were conducted between January 2008 and January 2009, which surveyed stretches of the Cuminá, Cuminapanema, Curuá, Jari, Mapuera, Nhamundá, and Paru rivers. These surveys yielded 286 species belonging to 38 families and eight orders, including seven new records of fish species for Brazil, six of which are also new records for the Amazon basin. Our results provide a valuable database for future research and conservation programs in the protected areas of the region.

https://doi.org/10.1590/1809-4392201901402 1698 downloads

14.

Peritonite piogranulomatosa por Nocardia concava em cão - relato de caso
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Trevisan, Y.P.A.

; Maruyama, F.H.

; Sonego, D.A.

; Souza, R.L.

; Almeida, A.B.P.F.

; Dutra, V.

; Nakazato, L.

; Colodel, E.M.

; Néspoli, P.B.

; Campos, C.G.

; Mendonça, A.J.

; Sousa, V.R.F.

Arquivo Brasileiro de Medicina Veterinária e Zootecnia

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oct 2019, Volume 71 N. 5 Pages 1518 - 1524

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO Nocardiose é causada por bactérias do gênero Nocardia do subgrupo Actinomycetos, que são Gram-positivas aeróbicas, filamentosas e podem apresentar ramificações. O diagnóstico baseia-se na presença de lesão inflamatória, com o microrganismo morfologicamente compatível, associada ao isolamento e à identificação microbiológica e molecular. Este trabalho tem por objetivo relatar um caso de nocardiose em canino, que desenvolveu inflamação piogranulomatosa peritoneal seis meses após ovariossalpingo-histerectomia. O animal apresentava hipertermia, distensão abdominal, taquipneia, polidipsia, hiporexia, mucosas hipocoradas e fezes pastosas. Os achados laboratoriais evidenciaram anemia leve e leucocitose por neutrofilia com desvio à esquerda e hipoalbuminemia. Uma massa na região mesogástrica e efusão peritoneal foram evidenciadas por meio da ultrassonografia abdominal. O líquido foi classificado como exsudato piogranulomatoso, e o animal submetido à laparotomia exploratória para lavagem abdominal e remoção da massa. Após procedimentos terapêuticos, ocorreu piora clínica e óbito. Peritonite piogranulomatosa foi a principal alteração anatomopatológica a qual foi associada à Nocardia spp. Molecularmente, a espécie isolada se aproxima da N. concava, por meio da análise filogenética. Essa espécie já foi descrita como causa de infecção em humanos na Ásia, no entanto não há registros na literatura na espécie canina, sendo este o primeiro relato.

ABSTRACT Nocardiosis is caused by an aerobic, gram-positive, ramificated and filamentous bacteria of the Nocardia genus, subgroup Actinomycetos. The diagnosis is based on the presence of the inflammatory lesions with the morphologically compatible microorganism associated with microbiological and molecular isolation and identification. The objective of this work is to report a case of nocardiosis in a canine that developed peritoneal pyogranulomatous inflammation six months after ovariosalpingohisterectomy. The animal had hyperthermia, abdominal distention, tachypnea, polydipsia, hyporexia, hypocorous mucosae and pasty feces. The laboratory findings revealed mild anemia and leukocytosis due to neutrophilia with left deviation and hypoalbuminemia. A mass in the mesogastric region and peritoneal effusion were evidenced by abdominal ultrasonography. The fluid was classified as pyogranulomatous exudate and the animal underwent exploratory laparotomy for abdominal lavage and mass removal. Despite the therapeutic procedures and clinical alterations the dog died. Piogranulomatous peritonitis was the main anatomopathological alteration which was associated with Nocardia spp. Molecularly, the isolated species approaches the N. concava species through phylogenetic analysis. This specie was described as a cause of infection in humans in Asia; however, there are no records in literature on the canine species, being this the first report.

https://doi.org/10.1590/1678-4162-10467 1072 downloads

15.

Equine pythiosis in the eastern wetlands of Uruguay
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Romero, Agustín

; García, Juan

; Balestié, Sofía

; Malfatto, Felipe

; Vicentino, Alejandra

; Sallis, Elisa Simone V.

; Schild, Ana Lucia

; Dutra, Fernando

Pesquisa Veterinária Brasileira

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jul 2019, Volume 39 N. 7 Pages 469 - 475

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO: Pitiose é uma doença granulomatosa e ulcerativa da pele dos equinos causada pelo oomyceto Pythium insidiosum (Pythiaceae). O objetivo deste trabalho foi descrever sete casos de pitiose equina que ocorreram de 2012 a 2017 na região leste do Uruguai. Seis dos sete casos ocorreram no ecossistema de áreas pantanosas da bacia da bacia da Lagoa Mirim Merin e o restante nas planícies fluviais pantanosas do rio Tacuarembó. As lesões se caracterizaram por tumores ulcerados de crescimento rápido com abundante tecido de granulação, secreção serossanguinolenta e presença de tratos fistulosos contendo material coraloide ou kunkers. Os equinos apresentavam prurido intenso, claudicação e perda da condição corporal e seis morreram ou foram eutanasiados in extremis. As principais lesões histológicas consistiam de um processo inflamatório piogranulomatoso com numerosos focos de necrose eosinofílicos (kunkers), colagenólise e reação de Splendori-Hoepli. Em todos os casos a impregnação pela prata (Grocott) revelou a presença de hifas intralesionais compatíveis com P. insidiosum, o que foi confirmado pela imuno-histoquímica em três casos. Um equino em fase terminal da doença foi tratado com triamcinolona acetonida (50mg, IM, a cada 15 dias), recuperando-se completamente após um ano. Conclui-se que a pitiose é uma enfermidade presente em áreas úmidas na região leste do Uruguai e o tratamento com triamcinolona pode ser uma alternativa promissora.

ABSTRACT: Equine pythiosis is an ulcerative and granulomatous disease of the skin, caused by the oomycete Pythium insidiosum (Pythiaceae). The objective of this study was to describe seven cases of equine pythiosis that occurred from 2012 to 2017 in the eastern region of Uruguay. Six of the seven cases occurred in the eastern wetland ecosystems of the Merin basin, and the remaining case occurred in the wetland fluvial plains of the Tacuarembó River. Lesions consisted of a large, rapidly growing ulcerated tumor with abundant granulation tissue, serosanguineous secretion, and fistulous tracts containing large concretions or kunkers. The animals presented intense pruritus, claudication and loss of body condition, with death or euthanasia in extremis in six cases. The main histological lesions consisted of an eosinophilic and pyogranulomatous inflammatory process, with numerous foci of eosinophilic necrosis (kunkers), collagenolysis, and a Splendore-Hoeppli reaction. In all cases, silver coloration (Grocott) showed intralesional hyphae compatible with P. insidiosum, which was confirmed by immunohistochemistry in three cases. A horse in the terminal phase of the disease was treated with triamcinolone acetonide (50mg IM every 15 days), and fully recovered after 1 year. It is concluded that equine pythiosis is prevalent in the wetland ecosystems of eastern Uruguay and that treatment with triamcinolone is auspicious.

https://doi.org/10.1590/1678-5150-pvb-6256 1029 downloads

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