This work developed an effective and eco-friendly analytical method to support quality control for Brazilian Bill proposal No. 5332/2009. The bill advocates the creation of the “Brazilian bread” by mandating the incorporation of cassava byproducts into wheat flour, with the aim of reducing Brazil’s external dependency on wheat imports and promoting domestic cassava family farming. For this, a handheld near-infrared (NIR) spectrophotometer was utilized for on-site determination of cassava starch in commercial wheat flour. The quantitative analysis, which utilized NIR spectra pre-processed with offset correction (OFF) combined with linear baseline correction (LBC), alongside the successive projections algorithm for interval selection in partial least squares (iSPA-PLS), exhibited superior predictive capability, achieving a relative error of prediction below 8%. Furthermore, the classification process, which involved NIR spectra pre-processed with LBC and iSPA-PLS for discriminant analysis (iSPA-PLS-DA), yielded an impressive 94% overall accuracy, with only 3 misclassifications in the test set. ecofriendly eco friendly No 53322009 5332 2009 5332/2009 bread flour Brazils Brazil s farming this nearinfrared near infrared (NIR onsite site preprocessed pre processed OFF (OFF LBC, , (LBC) iSPAPLS, iSPAPLS iSPA PLS (iSPA-PLS) capability 8 8% Furthermore process iSPAPLSDA, iSPAPLSDA DA (iSPA-PLS-DA) 94 accuracy set 5332200 533 200 5332/200 (LBC (iSPA-PLS (iSPA-PLS-DA 9 533220 53 20 5332/20 53322 5 2 5332/2 5332/

Resumo As armadilhas extracelulares de neutrófilos (NETs) foram relatadas pela primeira vez como uma estratégia microbicida para neutrófilos ativados. Por meio de uma resposta imunológica contra vários estímulos, os neutrófilos liberam seu DNA ligado a proteínas de grânulos, núcleo e citoplasma (por exemplo, elastase e mieloperoxidase). Até o momento, os NETs têm sido implicadas em danos aos tecidos durante processos inflamatórios intensos, principalmente quando sua liberação depende da geração de radicais de oxigênio. Os flavonóides são agentes antioxidantes e anti-inflamatórios, e destes, a quercetina é comumente encontrada em nossa dieta diária. Portanto, a quercetina pode exercer alguma atividade protetora contra o dano tecidual induzido por NETs. Em nossos ensaios in vitro, a quercetina reduziu a liberação das NETs, mieloperoxidase (MPO) e elastase a partir de neutrófilos estimulados com forbol 12-miristato 13-acetato (PMA). A atividade dessas enzimas também foi diminuída na presença de quercetina. A quercetina também reduziu o efeito citotóxico dos NETs sobre células alveolares (linha celular A549). Além disso, os ensaios in silico indicaram interações favoráveis entre a quercetina e as proteínas da NET (MPO e elastase). No geral, nossos resultados demonstram que a quercetina diminui os efeitos deletérios das NETs, reduzindo sua liberação à partir de neutrófilos ativados e diminuindo a atividade enzimática de MPO e elastase, possivelmente por meio de interação direta.

Abstract Neutrophil extracellular traps (NETs) were first reported as a microbicidal strategy for activated neutrophils. Through an immunologic response against several stimuli, neutrophils release their DNA together with proteins from granules, nucleus, and cytoplasm (e.g., elastase and myeloperoxidase). To date, NETs have been implicated in tissue damage during intense inflammatory processes, mainly when their release is dependent on oxygen radical generation. Flavonoids are antioxidant and anti-inflammatory agents; of these, quercetin is commonly found in our daily diet. Therefore, quercetin could exert some protective activity against tissue damage induced by NETs. In our in vitro assays, quercetin reduced NETs, myeloperoxidase (MPO), and elastase release from neutrophils stimulated with phorbol 12-myristate 13-acetate (PMA). The activity of these enzymes also decreased in the presence of quercetin. Quercetin also reduced the cytotoxic effect of NETs on alveolar cells (A549 cell line). Further, in silico assays indicated favorable interactions between quercetin and NET proteins (MPO and elastase). Overall, our results demonstrate that quercetin decreases deleterious cellular effects of NETs by reducing their release from activated neutrophils, and diminishing the enzymatic activity of MPO and elastase, possibly through direct interaction.

Resumo No atual contexto de patógenos fúngicos resistentes emergentes tais como Candida albicans e Candida parapsilosis, a descoberta de novos agentes antifúngicos é uma questão urgente. Esta pesquisa teve como objetivo avaliar o potencial antifúngico da 2-cloro-N-fenilacetamida contra cepas clínicas de C. albicans e C. parapsilosis resistentes a fluconazol. A atividade antifúngica da substância foi avaliada in vitro através da determinação da concentração inibitória mínima (CIM), concentração fungicida mínima (CFM), ruptura e inibição da formação de biofilme, ensaios de sorbitol e ergosterol, e associação entre esta molécula e antifúngicos comuns, anfotericina B e fluconazol. O produto teste inibiu todas as cepas de C. albicans e C. parapsilosis, com uma CIM variando de 128 a 256 µg.mL-1, e uma CFM de 512-1,024 µg.mL-1. Também inibiu até 92% da formação de biofilme e causou a ruptura de até 87% de biofilme pré-formado. A 2-cloro-N-fenilacetamida não promoveu atividade antifúngica pela ligação ao ergosterol da membrana celular fúngica, tampouco danificou a parede celular. Antagonismo foi observado ao combinar esta substância com anfotericina B e fluconazol. A substância exibiu atividade antifúngica significativa ao inibir tanto as células planctônicas quanto o biofilme das cepas resistentes ao fluconazol. Sua combinação com outros antifúngicos deve ser evitada e seu mecanismo de ação deve ser estabelecido.

Abstract In the current context of emerging drug-resistant fungal pathogens such as Candida albicans and Candida parapsilosis, discovery of new antifungal agents is an urgent matter. This research aimed to evaluate the antifungal potential of 2-chloro-N-phenylacetamide against fluconazole-resistant clinical strains of C. albicans and C. parapsilosis. The antifungal activity of 2-chloro-N-phenylacetamide was evaluated in vitro by the determination of the minimum inhibitory concentration (MIC), minimum fungicidal concentration (MFC), inhibition of biofilm formation and its rupture, sorbitol and ergosterol assays, and association between this molecule and common antifungal drugs, amphotericin B and fluconazole. The test product inhibited all strains of C. albicans and C. parapsilosis, with a MIC ranging from 128 to 256 µg.mL-1, and a MFC of 512-1,024 µg.mL-1. It also inhibited up to 92% of biofilm formation and rupture of up to 87% of preformed biofilm. 2-chloro-N-phenylacetamide did not promote antifungal activity through binding to cellular membrane ergosterol nor it damages the fungal cell wall. Antagonism was observed when combining this substance with amphotericin B and fluconazole. The substance exhibited significant antifungal activity by inhibiting both planktonic cells and biofilm of fluconazole-resistant strains. Its combination with other antifungals should be avoided and its mechanism of action remains to be established.

RESUMO Objetivou-se avaliar o efeito da fertirrigação pulsada e contínua sobre o acúmulo e a exportação de nutrientes pelo feijãocaupi. O delineamento experimental adotado foi em blocos ao acaso com 12 tratamentos distribuídos em esquema fatorial 5 x 2+ 2, com quatro repetições, sendo cinco lâminas de reposição da ETc equivalentes (40, 60, 80, 100 e 120%), combinadas com dois tipos de aplicação de fertirrigação (pulsada e contínua), mais dois tratamentos adicionais os quais foram irrigados com lâmina de 100% da ETc de forma pulsada e contínua e adubado convencionalmente. Os nutrientes analisados foram N, P, K, Ca, Mg, S, Fe, Cu, Mn e Zn. Os maiores acúmulos e exportações de nutrientes foram obtidos com lâminas de fertirrigação pulsada menores que 100% da ETc. O tratamento que recebeu adubação convencional e irrigação pulsada com lâmina de 100% da ETc promoveu maiores acúmulos e exportação de nutrientes em detrimento da irrigação contínua. A ordem decrescente de acúmulo de nutrientes para ambos os tipos de aplicação da fertirrigação (pulsada e contínua) foi: N > K > Ca > P > Mg > S > Fe > Mn > Zn > Cu. A ordem decrescente de exportação de nutrientes pelas vagens e grãos do feijão-caupi, para aplicação da fertirrigação pulsada foi: N > P > Ca > K > Mg > S > Fe > Zn > Mn > Cu. Já a aplicação da fertirrigação contínua foi: N > P > Ca > Mg > S> K> Fe > Zn > Mn > Cu. Objetivouse Objetivou se feijãocaupi 1 2 repetições 40, 40 (40 60 80 10 120%, 120 120% , 120%) contínua, convencionalmente Cu feijãocaupi, feijão caupi, caupi feijão-caupi 4 (4 6 8 (

ABSTRACT The objective was to evaluate the effects of pulse and continuous fertigation on the accumulation and export of nutrients by cowpea. The experimental design adopted was randomized blocks with 12 treatments distributed in a 5 x 2 + 2 factorial scheme, with four replicates, with five ETc replacement depths (40, 60, 80, 100 and 120%), combined with two types of fertigation application (pulse and continuous), plus two additional treatments under pulse (control 1) and continuous (control 2) irrigation with 100% ETc depth and conventional fertilization. The analyzed nutrients were N, P, K, Ca, Mg, S, Fe, Cu, Mn and Zn. Highest accumulations and exports of nutrients were obtained with pulse fertigation depths lower than 100% ETc. Conventional fertilization and pulse irrigation with 100% ETc depth promoted greater accumulation and export of nutrients compared to continuous irrigation. The descending order of nutrient accumulation for both types of fertigation application (pulse and continuous) was: N > K > Ca > P > Mg > S > Fe > Mn > Zn > Cu. The descending order of nutrient export by cowpea pods and grains was N > P > Ca > K > Mg > S > Fe > Zn > Mn > Cu for pulse fertigation and N > P > Ca > Mg > S > K > Fe > Zn > Mn > Cu for continuous fertigation. 1 scheme replicates 40, 40 (40 60 80 10 120%, 120 120% , 120%) continuous, control 4 (4 6 8 (

Resumo A produção de pitaia vem-se expandindo em várias regiões do Brasil apesar da carência de informações técnicas sobre o manejo das plantas. Para obter boa produtividade na cultura, o controle de plantas daninhas é fundamental; porém, o uso de herbicidas pode causar fitotoxicidade e redução do crescimento e da produção das plantas. Assim, o presente trabalho teve como objetivo avaliar atolerância de mudas de pitaia de polpa vermelha (Selenicereus polyrhizus) e polpa branca (S.undatus) a diferentes herbicidas aplicados em pós-emergência. Foram instalados doisexperimentos: um com pitaia-vermelha e outro com pitaia-branca, em 15 de agosto de 2020, no Setor de Fruticultura da Universidade Federal de Lavras. Os experimentos foramdispostos em delineamento em blocos casualizados, com quatro e três repetições, respectivamente, oito tratamentos e três plantas por parcela, em ambos os experimentos. Ostratamentos foram constituídos por testemunha, fomesafem, glufosinato de amônio, glifosato,cletodim, carfentrazona-etílica, imazetapir e clorimurom. Foram avaliados sintomas visuais defitotoxicidade aos 7; 14; 21 e 28 dias após a aplicação dos herbicidas. Aos 30 e 60 dias após a aplicação,avaliaram-se a porcentagem de necrose, comprimento dos cladódios e o número de brotos novos. O herbicida fomesafem causou maiores danos às mudas depitaia-vermelha,e fomesafem e glifosato causaram maiores danos às mudas de pitaia-branca. Paraambas as espécies de pitaias, o herbicida cletodim pode ser uma alternativa paracontrole de plantas daninhas de folha estreita sem provocar danos visuais no crescimento e nodesenvolvimento das plantas de pitaia. vemse vem se cultura fundamental porém Assim Selenicereus polyrhizus S.undatus Sundatus S undatus (S.undatus pósemergência. pósemergência pós emergência. emergência pós-emergência doisexperimentos pitaiavermelha pitaiabranca, pitaiabranca branca, pitaia-branca 1 2020 Lavras casualizados repetições respectivamente parcela testemunha amônio glifosatocletodim glifosato,cletodim carfentrazonaetílica, carfentrazonaetílica carfentrazona etílica, etílica carfentrazona-etílica clorimurom 7 14 2 3 6 aplicação,avaliaramse aplicaçãoavaliaramse aplicação,avaliaram avaliaram necrose novos depitaiavermelha,e depitaiavermelhae depitaia vermelha,e pitaiabranca. branca. pitaias 202 avaliaramse aplicaçãoavaliaram depitaiavermelha vermelhae 20

Abstract Pitaya production has been expanding in several regions of Brazil despite the lack of technical information on plant management. To obtain good crop productivity, weed control is essential; however, the use of herbicides can cause phytotoxicity and reduce plant growth and production. Thus, the present work aimed to evaluate the tolerance of red (Selenicereus polyrhizus) and white (S. undatus) pitaya seedlings to different herbicides applied after emergence. Two experiments were installed, one with red pitaya and the another with white pitaya, on August 15, 2020 in the Fruit Production Sector of the Federal University of Lavras. Experiments were arranged in a randomized block design, with four and three replicates, respectively, eight treatments and three plants per plot in both experiments. Treatments consisted of control, fomesafen, ammonium glufosinate, glyphosate, clethodim, carfentrazone-ethyl, imazethapyr and chlorimuron. Visual phytotoxicity symptoms were evaluated at 7, 14, 21 and 28 days after herbicide application. At 30 and 60 days after application, percentage of necrosis, cladode length and number of new shoots were evaluated. Herbicide fomesafen caused greater damage to red pitaya seedlings and fomesafen and glyphosate caused greater damage to white pitaya seedlings. For both pitaya species, herbicide clethodim can be an alternative for controlling narrow-leaf weeds without causing visual damage to the growth and development of pitaya plants. management productivity essential however Thus Selenicereus polyrhizus S. S (S undatus emergence installed 15 202 Lavras design replicates respectively glufosinate carfentrazoneethyl, carfentrazoneethyl carfentrazone ethyl, ethyl carfentrazone-ethyl chlorimuron 7 14 2 application 3 6 necrosis species narrowleaf narrow leaf 1 20

ABSTRACT Women living with human immunodeficiency virus are at an increased risk of developing cancers related to human papillomavirus (HPV). Thus, it is important to combine clinical assessments, serological screening, and HPV data for planning prevention policies. This study aimed to identify HPV and its specific types in the cervical, anal, and oral mucosa of HIV-seropositive women, associating it with viral load and lymphocyte count. Sociodemographic characteristics, health data (CD4+ and CD8+ T cell counts and viral load), and biological samples (cervical, anal, and oral) were collected from 86 HIV-positive women undergoing antiretroviral therapy. Data were classified according to the presence or absence of HPV-DNA, HPV-DNA presence at one or more anatomic sites, and level of oncogenic risk, considering low- and high-risk oncogenic HPV-DNA groups. The presence of HPV in the cervicovaginal site was 65.9%, 63.8% in anal canal, and 4.2% in oral mucosa. A viral load ≥75 HIV copies/mL was associated with the presence of HPV-DNA. There was an association between viral load and the low-risk HPV or high-risk HPV groups. We found a high prevalence of HPV infection in HIV-seropositive women, particularly in the cervical and anal mucosa, with viral load ≥75 HIV copies/mL being associated with HPV-DNA presence. HPV. . (HPV) Thus assessments screening policies HIVseropositive seropositive count characteristics CD4+ CD4 CD (CD4 CD8 load, , load) (cervical 8 HIVpositive positive therapy HPVDNA, HPVDNA DNA, DNA sites low highrisk groups 659 65 9 65.9% 638 63 63.8 canal 42 4 2 4.2 75 ≥7 copiesmL copies mL HPVDNA. DNA. lowrisk (HPV (CD 6 65.9 63. 4. 7 ≥ 65.

A novel, efficient, and cost-effective coating method was developed for fabricating highly conductive cotton yarn loaded with reduced graphene oxide (rGO). This method is straightforward, rapid, scalable, and amenable to solution processing. The key innovation involves pretreating the cotton yarn with chicken egg albumin (CEA) before applying the rGO coating through a simple dip-and-dry process. This method significantly reduces the electrical resistance of the cotton yarn, achieving an optimal specific resistance of 80 Ω.g/cm2 with just five coating cycles. Additionally, the coated samples exhibit exceptional reliability, enduring 104 bending tests and 5 washing tests without compromising conductivity. Notably, the study reveals consistent proportional variations in response to changes in relative humidity, regardless of the initial reference resistance value or yarn characteristics. These stable and conductive rGO-coated cotton yarns (rGOCY) might be seamlessly integrated into textiles and garments, enabling the development of flexible and wearable electronics, as well as sensors. novel efficient costeffective cost effective rGO. . (rGO) straightforward rapid scalable processing CEA (CEA dipanddry dip dry process 8 Ωgcm2 Ωgcm Ω g cm2 cm Ω.g/cm cycles Additionally reliability 10 conductivity Notably humidity characteristics rGOcoated rGOCY (rGOCY garments electronics sensors (rGO 1

Resumo O presente estudo buscou avaliar o potencial antimicrobiano e antiaderente do óleo essencial de Eucalipto radiata frente às cepas de veiculação alimentar de Escherichia coli. O estudo foi realizado usando a Concentração Inibitória Mínima (CIM) e Concentração Bactericida Mínima (CBM). Além disso, a técnica de difusão em disco foi usada para avaliar a associação do óleo essencial de Eucalipto radiata com antimicrobianos sintéticos. A Concentração Inibitória Mínima de Aderência (CIMA) também foi realizada. Os resultados revelaram que E. radiata apresentou atividade antimicrobiana contra as cepas de E. coli testadas, com valores de CIM variando entre 500 μg/mL a 1000 μg/mL e valores de CBM variando entre 500 μg/mL a 1,024 μg/mL. Quanto às associações, observou-se que o óleo de E. radiata exibiu efeito sinérgico para alguns antibióticos, sobretudo a Ceftriaxona, apresentando maior interferência do óleo essencial. Além disso, foi eficaz na inibição a aderência das cepas bacterianas de E. coli, mostrando um efeito antibiofilme mais significativo que o agente antibacteriano digluconato de clorexidina a 0.12%. Em resumo, o óleo essencial de E. radiata apresentou potencial antimicrobiano contra cepas de E. coli de origem alimentar, podendo assim, mediante estudos aprofundados, ser utilizado isoladamente ou em associação com antimicrobianos sintéticos para combater infecções causadas por esse patógeno. (CIM CBM. . (CBM) disso CIMA (CIMA realizada E testadas 50 μgmL μg mL 100 1024 1 024 1,02 associações observouse observou se antibióticos Ceftriaxona 012 0 12 0.12% resumo assim aprofundados patógeno (CBM 5 10 102 02 1,0 01 0.12 1, 0.1 0.

Abstract The present study sought to evaluate the antimicrobial and anti-adherent potential of Eucalyptus radiata essential oil against food-borne strains of Escherichia coli. The study was performed using the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC). In addition, the disk diffusion technique was used to evaluate the association of Eucalyptus radiata essential oil with synthetic antimicrobials. The Minimum Inhibitory Adherence Concentration (MIC) was also performed. The results revealed that E. radiata showed antimicrobial activity against the E. coli strains tested, with MIC values ranging from 500 μg/mL to 1000 μg/mL and MBC values ranging from 500 μg/mL to 1,024 μg/mL. As for the associations, it was observed that E. radiata oil exhibited a synergistic effect for some antibiotics, especially Ceftriaxone, with greater interference from the essential oil. Furthermore, it was effective in inhibiting the adherence of bacterial strains of E. coli, showing a more significant antibiofilm effect than the antibacterial agent 0.12% chlorhexidine digluconate. In summary, the essential oil of E. radiata showed antimicrobial potential against strains of E. coli of food origin, and can therefore, through in-depth studies, be used alone or in association with synthetic antimicrobials to combat infections caused by this pathogen. antiadherent anti adherent foodborne borne (MIC MBC. . (MBC) addition E tested 50 μgmL μg mL 100 1024 1 024 1,02 associations antibiotics Ceftriaxone Furthermore 012 0 12 0.12 digluconate summary origin therefore indepth depth studies pathogen (MBC 5 10 102 02 1,0 01 0.1 1, 0.

ABSTRACT Rocky Mountain Spotted Fever is a rickettsial disease caused by the bacteria Rickettsia rickettsii. In Brazil, the disease is known as Brazilian spotted fever (BSF), being the most significant tick-borne disease in the country. Among the affected patients, only 5% of cases occur in children aged one to nine years. Typical symptoms of the disease are fever, rash, headache and digestive symptoms. Neurological manifestations such as seizures, aphasia and hemiparesis have been described in few patients. This study aimed to describe the case of an infant diagnosed with BSF who presented severe signs of neurological manifestation. rickettsii Brazil BSF, , (BSF) tickborne tick borne country patients 5 years rash seizures manifestation (BSF

ABSTRACT Yellow fever vaccine (YFV) is a live attenuated vaccine that can cause a mild infection in immunocompetent patients. However, it may not be self-limiting in patients with inborn errors of immunity (IEI) and may be the first and most severe presentation in these patients. A 10-month-old female infant sought emergency care presenting fever for three days and diffuse exanthema. She was a previous healthy child of consanguineous parents. The child had received YFV 28 days before the onset of symptoms. Upon hospital admission, petechial rash on the limbs and hepatosplenomegaly were noted on physical exam. Laboratory tests showed thrombocytopenia, increased serum aminotransferases and elevated gamma-glutamyl transferase (GGT) and alkaline phosphatase levels. During hospitalization she developed hypoactivity, drowsiness, and hypotonia. The possibility of viscerotropic and neurotropic vaccine associated disease was suspected and a possible primary immunodeficiency disease considered. The patient was tested for antibodies against the yellow fever virus (MAC ELISA) on serum and cerebrospinal fluid (CSF) samples, showing positive IgM results. Immunophenotyping showed low levels of lymphocytes and absence of T-cell receptor excision circles (TREC), leading to diagnose of severe combined immunodeficiency disease (SCID). Despite treatment, after 35 days of hospitalization, she evolved to cardiorespiratory arrest and death. Serious adverse events after administration of the YFV are rare and associated with neurological or visceral involvement in most cases. The unfavorable outcome highlights the importance of neonatal screening for SCID and the clinical suspicion of primary immunodeficiencies in infants who have serious adverse events to live virus vaccines. (YFV However selflimiting self limiting IEI (IEI 10monthold monthold 10 month old exanthema parents 2 symptoms admission exam thrombocytopenia gammaglutamyl gamma glutamyl GGT (GGT hypoactivity drowsiness hypotonia considered MAC ELISA CSF (CSF samples results Tcell T cell TREC, TREC , (TREC) SCID. . (SCID) treatment 3 death cases vaccines 1 (TREC (SCID

This work aims to determine the best substrate preparation and conversion coatings that may enhance the anti-corrosive performance of alumina film/stainless steel substrate systems in Ringer’s solution. Boehmite, silica, silica/boehmite, and boehmite/silica conversion coatings were deposited on sanded or sandblasted 304L stainless steel samples, and the best results were further covered with alumina film for a long-time exposure evaluation. The best anti-corrosive performances for each substrate preparation were observed for the sandblasted sample covered with silica/boehmite conversion coating (JSB500) and the sanded sample covered with silica (LS500), which showed the highest global resistance (Rg) values (828 Ω.cm2 and 1837 Ω.cm2, respectively) and the smallest values of global capacitance, Cg (2.19 X 10-6 F.cm-2 and 3.37 X 10-9 F.cm-2, respectively). After covering with the alumina film and evaluating for 216 h in the corrosive medium, it was noted an increase in the Rg values for both systems (JSBA500 and LSBA500), likely due to the presence of a corrosion products layer. The presence of iron oxides in these conversion coatings led to a more stable alumina film. Comparing the selected coating systems, the LSBA500 exhibited an improved performance, reaching an increase of 85% in Rg and a decrease of 94% in Cg. anticorrosive anti filmstainless Ringers Ringer s solution Boehmite silicaboehmite boehmite boehmitesilica L samples longtime long time evaluation JSB500 JSB (JSB500 LS500, LS500 LS , (LS500) (Rg 828 (82 Ωcm2 Ωcm Ω cm2 cm Ω.cm 183 respectively capacitance 2.19 219 2 19 (2.1 106 10 6 10- F.cm2 Fcm2 Fcm F.cm F F.cm- 337 3 37 3.3 109 9 F.cm2, 2, respectively. . 21 medium JSBA500 JSBA (JSBA50 LSBA500, LSBA LSBA500) layer LSBA50 85 94 JSB50 (JSB50 LS50 (LS500 82 (8 18 2.1 1 (2. 33 3. JSBA50 (JSBA5 LSBA5 8 JSB5 (JSB5 LS5 (LS50 ( 2. (2 JSBA5 (JSBA (JSB (LS5 (LS

Resumo A Serra do Ibitipoca ocorre no sudeste do estado de Minas Gerais, sudeste do Brasil, e inclui um mosaico de diferentes tipos de vegetação, como parte do domínio da Mata Atlântica. Tal heterogeneidade é resultado da ocorrência de diversos ecótonos na região, considerados áreas de amortecimento essenciais para manutenção da biodiversidade e estrutura de ecossistemas adjacentes. Dada a importância destes ambientes para conservação da biodiversidade, inventários florísticos são importantes para catalogar a riqueza de plantas em áreas naturais, onde espécies e paisagens têm sido destruídas, especialmente nas últimas décadas. Para contribuir com o aumento do conhecimento sobre a Flora vascular na Serra do Ibitipoca, um inventário florístico foi conduzido em áreas privadas adjacentes ao Parque Estadual do Ibitipoca. As descobertas mais relevantes do presente estudo incluem: caracterização dos diferentes tipos de vegetação, 17 novos registros para a Flora de Minas Gerais, coleta de 288 espécies nunca registradas para o parque (80% de dissimilaridade – especialmente devido à ocorrência e tamanho de diferentes fitofisionomias entre as áreas) e presença de 31 espécies ameaçadas. Além disso, discussões sobre esforços para conservação e políticas públicas são apresentadas. Gerais Brasil vegetação Atlântica região naturais destruídas décadas incluem 1 28 80% 80 (80 3 ameaçadas disso apresentadas 2 8 (8 (

Abstract The Ibitipoca Mountains occur in southeastern Minas Gerais state, Southeast Brazil, and includes a mosaic of different vegetation types, as part of the Atlantic Forest domain. Such heterogeneity results in the occurrence of several ecotones in the region, considered essential buffer zones for maintaining biodiversity and structure among adjacent ecosystems. Given the importance of these environments for biodiversity conservation, floristic surveys are important to catalogue plant richness in natural areas, where species and landscapes have been destroyed, especially over the last decades. To contribute to increase the knowledge on the vascular Flora in the Ibitipoca Mountains, a floristic inventory was undertaken in private properties located in the boundaries of “Parque Estadual do Ibitipoca” (Ibitipoca State Park). Relevant findings of the present study include: characterization of the different vegetation types, 17 new records for the Flora of Minas Gerais, collection of 288 species never recorded in the state park (80% dissimilarity – especially due to the occurrence and size of different phytophysiognomies between these areas) and presence of 31 threatened species. In addition, discussions about conservation efforts and public policies are presented. Brazil types domain region ecosystems areas destroyed decades Parque Park. Park . Park) include 1 28 80% 80 (80 3 addition presented 2 8 (8 (

Abstract Objective This study aimed at investigating a set of peripheral cytokines in elderly female patients with MDD, comparing them to controls, and assessing the potential influence of clinical comorbidities on inflammatory markers. Methods Twenty-five elderly female patients diagnosed with MDD and 19 age-matched female controls were enrolled on this study. Plasma levels of interleukin (IL)-4, IL-6, IL-10, interferon (IFN)-γ and tumor necrosis factor (TNF)-α were evaluated with commercially-available assays. Results Elderly female patients with MDD exhibited higher plasma IL-6 and IL-4 levels when compared to controls. In a logistic regression model taking cytokine levels, comorbidities, and age into account, only type 2 diabetes mellitus (DM2) remained associated with MDD. Conclusion Diabetes influences the association between MDD and higher levels of cytokines in elderly female patients. Future studies should take this evidence into account in order to mitigate confounding factors. markers Twentyfive Twenty five 1 agematched matched IL4, IL4 IL 4, 4 (IL)-4 IL6, IL6 6, 6 IL10, IL10 10, 10 IL-10 IFNγ IFN γ TNFα TNF α commerciallyavailable commercially available assays IL- DM2 DM (DM2 factors (IL)- IL1 IL-1 (DM (IL) (IL

ABSTRACT Streptococcus suis has been widely reported as a pathogen in animals, especially pigs. In terms of human health implications, it has been characterized as a zoonosis associated with the consumption of pork products and occupational exposure, particularly in Southeast Asian countries. Here, we present a rare case of human S. suis infection in Brazil, diagnosed in an older adult swine farmer, a small rural producer residing in the semi-arid region of Bahia, Brazil. animals pigs implications exposure countries Here S Brazil farmer semiarid semi arid Bahia

ABSTRACT This study aims to investigate the possible association between neutrophil/lymphocyte and platelet/lymphocyte ratio in women with HIV, undergoing antiretroviral treatment, with HPV coinfection. This is a cross-sectional study with HIV positive women; their biological samples were collected for laboratory tests (complete blood count) and oncotic cytology for detection of HPV DNA, by PCR-Nested (PGMY and GP primers). Viral load and CD4 and CD8 T-cells counts were obtained from medical records. The data were analyzed, comparing the two groups: those with coinfection and those without it. From 82 HIV seropositive women, 50% exhibited HPV coinfection and 12.2% of coinfected patients had cervical cell alterations. Quantification of viral load, CD4 and CD8 T-cells count, CD4 / CD8 ratio and neutrophil/lymphocyte (NLR) and platelet/lymphocyte (PLR) ratio presented significant differences between groups (p<0.05). The predicting power of NLR and PLR in differentiating HIV/HPV coinfection which demonstrated differences between groups (AUC of 0.882 and 0.776 for NLR and PLR, respectively). There is a relation between the neutrophil/lymphocyte and platelet/lymphocyte ratio with HIV/HPV coinfection in women undergoing antiretroviral treatment, suggesting a state of greater and persistent systemic inflammation, reflecting as a biomarker for screening and monitoring these patients. neutrophillymphocyte neutrophil lymphocyte plateletlymphocyte platelet treatment crosssectional cross sectional complete count DNA PCRNested PCR Nested PGMY primers. primers . primers) CD Tcells T cells records analyzed it 8 50 122 12 2 12.2 alterations (NLR (PLR p<0.05. p005 p p<0.05 0 05 (p<0.05) HIVHPV AUC 0882 882 0.88 0776 776 0.77 respectively. respectively respectively) inflammation 5 1 12. p00 p<0.0 (p<0.05 088 88 0.8 077 77 0.7 p0 p<0. (p<0.0 08 0. 07 7 p<0 (p<0. p< (p<0 (p< (p