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Abstract The structural condition of the cable-stayed towers anchorage on power transmission lines requires constant monitoring. Maintenance routines must be able to identify faulty anchor rods and substitute them to avoid tower collapses and power delivery interruptions. Modern statistical diagnostic systems based on machine learning requires the generation of several distinct rod sample signals to be trained, which is a time-consuming process in typical electromagnetic solvers. Aiming to generate samples in a feasible time span, this paper presents a modeling strategy based on the cascade analysis of the transmission line composed by the anchor and the reference rods, interconnected by a dedicated high-frequency connector. Each distinct transmission line part is modeled by a quadrupole as a partial ABCD matrix. The complete response for each experimental setup is obtained from the cascaded-multiplying of the partial matrices. The proposed modeling proved to be accurate and provides a faster way to obtain the S-parameters from distinct faulty rods if compared to the traditional methodologies using electromagnetic tools.

https://doi.org/10.1590/2179-10742022v21i1253757

Temporal monitoring of contamination in three sandy beaches from the 2019 oil spill near Cabo de Santo Agostinho, Northeastern Brazil
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BONTEMPO FILHO, EDUARDO B.

; COUTINHO, ROBERTO Q.

; BARBOSA, JOSÉ ANTONIO

; BARCELLOS, ROBERTO L.

; GIACHETI, HERALDO LUIZ

; RAMOS, GERMANO MÁRIO S.

Anais da Academia Brasileira de Ciências

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2022, Volume 94 elocation e20210513

Abstract: En | Text: En | PDF: En

Abstract In 2019, a massive oil spill impacted coastal ecosystems in eleven Brazilian states; these ecosystems were diverse with regard to their different geological settings (sandy beaches, rocky coasts, estuaries, tidal flats, mangroves, and reef systems) being impacted. Here, we present a temporal analysis on the occurrence of remaining contaminants on three beaches (Paiva, Itapuama, and Enseada) of the Cabo de Santo Agostinho City, Pernambuco State. The approach focuses on the systematic mapping of residues, collection of sediment samples, photographic documentation, and qualitative microscopic analysis. Grain size, calcium carbonate, and total organic matter content analyses provided a faciological and energetic characterization of the environment. The objective was to verify the relationship between the remaining contaminants and the geological setting. The results showed intense weathering of the remaining oil residues due to the high energy environment. We found tar balls reworked by seasonal erosion on the three beaches and no tar residues in shallow subsurface sediments. A large quantity of tar residues remains on rock surfaces in Itapuama Beach, which bears the higher permanence of contamination because it is less affected by weathering and beach erosion. This investigation allowed for an impact assessment of the geological-morphological characteristics of the beaches.

https://doi.org/10.1590/0001-3765202220210513

Clinical, laboratory and anatomopathological study of caudal vena cava thrombosis in cattle
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Coutinho, Luiz T.

; Santos, Jomel F.

; Souto, Rodolfo José C.

; Silva, Nivan A.A.

; Souza, José Cláudio A.

; Mendonça, Carla L.

; Cajueiro, Jobson Filipe P.

; Afonso, José Augusto B.

Pesquisa Veterinária Brasileira

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2021, Volume 41 elocation e06856

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO: Este trabalho teve por objetivo descrever os principais achados clínico-epidemiológicos, laboratoriais e anatomopatológicos de 10 bovinos diagnosticados com trombose da veia cava caudal. Os principais achados clínicos foram redução da produção leiteira, diminuição do apetite, apatia, comprometimento da dinâmica ruminal, alterações cardio-respiratórias (taquicardia e taquipnéia), epistaxe, hemoptise e ascite. Foi constatada a ocorrência de doenças intercorrentes como mastite, metrite e flebite. O hemograma revelou discreta anemia, leucocitose por neutrofilia com desvio para esquerda regenerativo e hiperfibrinogenemia. No exame anatomopatológico revelou trombos localizados na veia cava caudal, hepatomegalia, ascite e abscessos hepáticos; além de abscessos, enfisema e edema pulmonares. A associação de informações epidemiológicas, sinais clínicos como desconforto respiratório, epistaxe ou hemoptise, além de anemia e leucocitose por neutrofilia, bem como a ocorrência de trombo na veia cava caudal como achados patológicos são elementos indicativos do quadro clínico de trombose de veia cava em bovinos. Reitera-se que essa doença tem prognóstico desfavorável e, quando diagnosticada, o animal deve ser abatido.

ABSTRACT: This paper aimed to describe the main clinico-epidemiological, laboratory, and anatomopathological findings in 10 cattle affected with caudal vena cava thrombosis. The main clinical signs observed were decreased milk production, reduced appetite, apathy, impairment of ruminal motility, cardiorespiratory disorders (tachycardia and tachypnea), epistaxis, hemoptysis, and ascites. Intercurrent diseases such as mastitis, metritis, and phlebitis were verified. The hematological findings were mild anemia, leukocytosis due to neutrophilia with regenerative left shift, and hyperfibrinogenemia. The pathological exams revealed thrombi in the caudal vena cava, hepatomegaly, ascites, liver abscesses, pulmonary edema and emphysema, and abscesses in the lungs. The association of epidemiological information, clinical signs such as respiratory distress, epistaxis or hemoptysis, in addition to anemia and leukocytosis due to neutrophilia, as well as the occurrence of thrombus in the caudal vena cava as pathological findings, are indicative elements of the clinical picture of vena cava thrombosis in cattle. It is reiterated that this disease has an unfavorable prognosis and, when diagnosed, the animal must be culled.

https://doi.org/10.1590/1678-5150-pvb-6856 74 downloads

Clinical, laboratory, ultrasonographic, and anatomopathological aspects of 30 cases of traumatic reticulosplenitis in cattle
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Silva, Tatiane V.

; Cajueiro, Jobson Filipe P.

; Silva, Nivan Antônio A.

; Souto, Rodolfo José C.

; Coutinho, Luiz T.

; Mendonça, Carla L.

; Afonso, José Augusto B.

; Miranda Neto, Eldinê G.

Pesquisa Veterinária Brasileira

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sep 2020, Volume 40 N. 9 Pages 669 - 676

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO: A ingestão de corpos estranho de origem metálica e/ou pontiagudos desencadeia em bovinos, quadros de Reticuloperitonite Traumática e suas sequelas. Dentre as quais podemos destacar a retículo esplenite traumática cuja letalidade é elevada, embora a mesma apresente uma baixa frequência na clínica de ruminantes. Portanto, baseado na escassez de informações sobre esta enfermidade, este trabalho teve por objetivo avaliar os achados clínicos, laboratoriais, ultrassonográficos e anatomopatológicos de 30 bovinos adultos diagnosticados com retículo esplenite traumática. Os achados clínicos, ultrassonográfico e anatomopatológico foram analisados por meio de estatística descritiva, e os dados laboratoriais foram avaliados utilizando-se as medidas de tendência central. Clinicamente os animais apresentaram desidratação e alterações no comportamento, apetite e na motilidade ruminal. Os achados hematológicos revelaram leucocitose (37077.17±25004.59cell/μL) por neutrofilia com desvio à esquerda regenerativo e hiperfibrinogenemia (1130±364.98mg/dL). O exame ultrassonográfico possibilitou a visualização de filamentos móveis e ecogênicos que corresponderam à presença de aderências fibrinosas, observou-se também, deslocamento do retículo e irregularidade no seu contorno além das alterações na quantidade, padrão e amplitude das contrações reticulares. Permitiu ainda, a constatação de alterações esplênicas como abscessos que foram caracterizados como estruturas circulares de variados tamanhos delimitada por capsula contendo no seu interior conteúdo de ecogenicidade variável. Trombose da veia esplênica e dobramento do baço. Os resultados obtidos nesse trabalho, indicaram que a retículo esplenite traumática causa sinais clínicos inespecíficos, severas alterações laboratoriais e principalmente que a ultrassonografia é um método eficiente para o diagnóstico dessa enfermidade uma vez que as lesões anatomopatológicas confirmaram os achados ultrassonográficos.

ABSTRACT: Ingestion of metallic and/or sharp foreign bodies triggers cases of traumatic reticuloperitonitis and its sequelae in cattle. Among these sequelae, we can highlight traumatic reticulosplenitis, that has high mortality, although its frequency in the ruminant medicine is low. Therefore, based on the scarcity of information on this disease, the current study aimed to evaluate the clinical, laboratory, ultrasonographic, and pathological findings of 30 adult cattle diagnosed with traumatic reticulosplenitis. Clinical, ultrasound, and anatomopathological findings were analyzed using descriptive statistics and laboratory data were evaluated using measures of central tendency. Clinically the animals presented dehydration and alterations in behavior, appetite, and ruminal motility. Hematological findings revealed neutrophilic leukocytosis (37077.17±25004.59cell/μL) with regenerative left shift and hyperfibrinogenemia (1130±364.98mg/dL). The ultrasound examination enabled visualization of mobile and echogenic filaments that corresponded to the presence of fibrin adhesions. Displacement of the reticulum and irregularity in its contour, as well as alterations in the quantity, pattern, and amplitude of reticular contractions were also observed. Splenic alterations such as abscesses were found, characterized as circular structures of varying sizes delimited by capsules containing variable echogenicity. Splenic vein thrombosis and spleen folding were also observed. The results obtained in the current study indicated that traumatic reticulosplenitis causes nonspecific clinical signs, severe laboratory alterations and, mainly, that ultrasound is an efficient method for the diagnosis of this disease, since the anatomopathological lesions confirmed the ultrasound findings.

https://doi.org/10.1590/1678-5150-pvb-6743 570 downloads

3rd Guideline for Perioperative Cardiovascular Evaluation of the Brazilian Society of Cardiology
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Gualandro, Danielle Menosi

; Yu, Pai Ching

; Caramelli, Bruno

; Marques, André Coelho

; Calderaro, Daniela

; Fornari, Luciana Savoy

; Pinho, Claudio

; Feitosa, Alina Coutinho Rodrigues

; Polanczyk, Carisi Anne

; Rochitte, Carlos Eduardo

; Jardim, Carlos

; Vieira, Carolina L. Z.

; Nakamura, Debora Y. M.

; Iezzi, Denise

; Schreen, Dirk

; Adam, Eduardo Leal

; D’Amico, Elbio Antonio

; Lima, Emerson Q. de

; Burdmann, Emmanuel de Almeida

; Mateo, Enrique Indalecio Pachón

; Marcondes-Braga, Fabiana Goulart

; Machado, Fabio S.

; Paula, Flavio J. de

; Carmo, Gabriel Assis Lopes do

; Feitosa-Filho, Gilson Soares

; Prado, Gustavo Faibischew

; Lopes, Heno Ferreira

; Fernandes, João R. C.

; Lima, José J. G. de

; Sacilotto, Luciana

; Drager, Luciano Ferreira

; Vacanti, Luciano Janussi

; Rohde, Luis Eduardo Paim

; Prada, Luis F. L.

; Gowdak, Luis Henrique Wolff

; Vieira, Marcelo Luiz Campos

; Monachini, Maristela Camargo

; Macatrão-Costa, Milena Frota

; Paixão, Milena Ribeiro

; Oliveira Junior, Mucio Tavares de

; Cury, Patricia

; Villaça, Paula R.

; Farsky, Pedro Silvio

; Siciliano, Rinaldo F.

; Heinisch, Roberto Henrique

; Souza, Rogerio

; Gualandro, Sandra F.M.

; Accorsi, Tarso Augusto Duenhas

; Mathias Júnior, Wilson

Arquivos Brasileiros de Cardiologia

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sep 2017, Volume 109 N. 3 Pages 1 - 104

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

https://doi.org/10.5935/abc.20170140 100302 downloads

A citotoxicidade e a avaliação da atividade antiprotozoária de Melissa officcinalis L (CIDRO-MELISA)
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Rodrigues Costa, Adrielle

; Silvino Pereira, Pedro

; Marivando Barros, Luiz

; Duarte, Antônia Eliene

; Vega Gomez, Maria Celeste

; Rolón, Miriam

; Soares Vidal, Carlos Alberto

; Josicleide Maia, Ana

; Bezerra Morais Braga, Maria Flaviana

; Coutinho, Henrique Douglas

Revista Cubana de Plantas Medicinales

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dec 2016, Volume 21 N. 4 Pages 1 - 13

Abstract: Pt | Text: Pt | PDF: Pt

Prevalência de déficits motores e desordem coordenativa desenvolvimental em crianças da região Sul do Brasil
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Valentini, Nadia Cristina

; Coutinho, Mônia Tainá C.

; Pansera, Simone Maria

; Santos, Viviane Aparecida P. dos

; Vieira, José Luiz L.

; Ramalho, Maria Helena

; Oliveira, Marcio Alves de

Revista Paulista de Pediatria

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sep 2012, Volume 30 N. 3 Pages 377 - 384

Abstract: Pt En Es | Text: Pt En Es | PDF: Pt | PDF: En | PDF: Es

OBJETIVO: Investigar a prevalência de provável desordem coordenativa desenvolvimental e de seu risco e o desenvolvimento típico em meninos e meninas, com quatro a 12 anos de idade. MÉTODOS: Foram avaliadas 1.587 crianças da região Sul do Brasil com o Movement Assessment Battery for Children. Os participantes foram divididos em quatro grupos de acordo com a idade (G1, de quatro a seis anos; G2, de sete a oito; G3, de nove a dez; e G4, de 11 a 12). RESULTADOS: Ao todo, 19,9% das crianças foram identificadas com provável desordem coordenativa desenvolvimental (percentil ≤5%) e 16,8% com risco de tal desordem (percentil ≤15%), todas avaliadas pelo Movement Assessment Battery for Children. Houve interação significativa entre a classificação no Movement Assessment Battery for Children, por grupo de idade e sexo (p<0,0001). A análise por gênero demonstrou maior prevalência de desordem coordenativa desenvolvimental no grupo de meninas nas faixas etárias G3 e G4 (p<0,05). Observaram-se interações significativas para a destreza manual (p=0,0001), habilidades com bola (p<0,0001) e equilíbrio (p<0,0001). Destreza manual foi o item com maior peso nas variações observadas. CONCLUSÕES: As dificuldades nas tarefas de destreza manual repercutiram mais fortemente para o diagnóstico de provável desordem coordenativa desenvolvimental e no risco de tal desordem. Os meninos apresentaram pior desempenho nas tarefas de destreza manual e equilíbrio, enquanto as meninas apresentaram maior deficiência nas habilidades com bola. O desempenho motor deficitário foi mais prevalente no grupo etário de crianças mais velhas.

OBJECTIVE: To investigate the prevalence of probable developmental coordination disorder and its risk, and the typical development in boys and girls aged from four to 12 years-old. METHODS: 1,587 children from South Brazil were evaluated by the Movement Assessment Battery for Children. The participants were divided into four age groups (G1, from four to six years-old; G2, from seven to eight; G3, from nine to ten; and G4, from 11 to 12). RESULTS: 19.9% of the children were identified as having probable developmental coordination disorder (percentile ≤5%) and 16.8% were identified at risk of such disorder (percentile ≤15%), based on the Movement Assessment Battery for Children. Significant interaction was found for the classification of the Movement Assessment Battery for Children between age group and gender (p<0.0001). The gender analysis showed a higher prevalence of Developmental Coordination Disorder in girls at the age groups G3 and G4 (p<0.05). Significant interactions were found for manual dexterity (p=0.0001), ball skills (p<0.0001), and balance (p<0.0001). Manual dexterity was responsible for the highest variances observed. CONCLUSIONS: The motor difficulties in manual dexterity robustly accounted for the diagnosis of probable and at risk developmental coordination disorder. Boys presented lower level of performance in the manual dexterity and balance tasks, while girls of all age groups had more difficulties related to ball skills. Higher levels of motor impairment were found in older children.

OBJETIVO: Investigar la prevalencia de un posible trastorno coordinativo de desarrollo y su riesgo, y el desarrollo típico en niños y niñas, con 4 y 12 años de edad. MÉTODOS: Se evaluaron a 1.587 niños de la región Sur de Brasil con el Movement Assessment Battery for Children. Los participantes fueron divididos en cuatro grupos conforme a la edad (1, de los cuatro a los seis años; 2, de los siete a los ocho; 3, de los nueve a los diez; y 4, de los 11 a los 12). RESULTADOS: En total, 19,9% de los niños fueron identificados con probable trastorno coordinativo de desarrollo (percentil ≤ 5%) y 16,8% con riesgo de tal trastorno (percentil ≤ 15%), todos evaluados por el Movement Assessment Battery for Children. Hubo interacción significativa entre la clasificación en el Movement Assessment Battery for Children por grupo de edad y sexo (p<0,0001).El análisis por género demostró mayor prevalencia de trastorno coordinativo de desarrollo en el grupo de niñas en las franjas de edad 3 y 4 (p<0,05). Se observaron interacciones significativas para la destreza manual (p=0,0001), habilidades con balón (p<0,0001) y equilibrio (p<0,0001). Destreza manual fue el ítem con mayor peso en las variaciones observadas. CONCLUSIONES: Las dificultades en las tareas de destreza manual repercutieron más fuertemente para el diagnóstico de probable trastorno coordinativo de desarrollo y en riesgo de tal trastorno. Los niños presentaron peor desempeño en las tareas de destreza manual y equilibrio, mientras que las niñas presentaron mayor deficiencia en las habilidades con balón. El desempeño motor deficitario fue más prevalente en el grupo etario de niños mayores.

6874 downloads Cited 9 times in SciELO

Avaliação econômica da produção de alface americana em função de lâminas de irrigação
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Lima Junior, Joaquim Alves de

; Pereira, Geraldo Magela

; Geisenhoff, Luciano Oliveira

; Costa, Gustavo Guerra

; Reis, Ricardo Pereira

; Oliveira, Luiz Fernando Coutinho de

Ciência e Agrotecnologia

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apr 2011, Volume 35 N. 2 Pages 392 - 398

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Visando reduzir as dificuldades do produtor no cultivo da alface americana, especificamente quanto à falta de informações técnicas sobre o momento oportuno de irrigar e a quantidade de água a ser aplicada economicamente viável, objetivou-se estudar o efeito de diferentes lâminas de água sobre o comportamento produtivo e econômico da alface americana. O experimento foi realizado na Universidade Federal de Lavras, no período de março a maio de 2008 em casa de vegetação. O delineamento experimental foi em blocos casualizados, com cinco tratamentos e quatro repetições. Os tratamentos foram constituídos de cinco fatores de evaporação, correspondentes a 0,30, 0,60, 0,90, 1,20 e 1,50 EVm, baseado na lâmina evaporada de um minitanque. Os resultados permitiram concluir que, a máxima produtividade total e comercial, 65.578 e 35.308 kg ha-1, foram estimadas com aplicação de lâminas equivalentes a 203,9 e 204,3 mm, respectivamente, correspondente ao fator de reposição de 101%. Considerando o preço do fator água (R$ 0,67 mm-1) e o preço da alface americana (R$ 0,90 kg-1), a lâmina economicamente ótima foi 203,9 mm, resultando em uma produtividade comercial praticamente igual à máxima física.

With the objective of minimize the difficulties faced by the producers on cultivating the crisphead lettuce, specifically those related with the lack of technical information regarding the ideal moment to irrigate and the amount of water to be applied, a study was performed in order to determine the effect of different water depths on production and economical behavior of crisphead lettuce. The experiment was carried out in a greenhouse of the Universidade Federal de Lavras, between March and May 2008. A randomized block design with five treatments and four replicates was applied. The treatments, consisted of five evaporation factors 0.30, 0.60, 0.90, 1.20 and 1.50 EVm, based on an evaporated depth of a reduced pan. The results showed that the maximum total and commercial yield, 65.578 and 35.308 kg ha-1, were estimated by applying depths equivalent to 203.9 and 204.3 mm, respectively, corresponding to 101% replacement factor. Considering the water factor price (R$ 0.67 mm-1) and crisphead lettuce price (R$ 0.90 kg-1), the economically optimum depth was 203.9 mm, which resulted in a commercial yield equal to a physical maximum.

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Rizóbios nativos do Rio Grande do Sul simbioticamente eficientes em Lotus glaber
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Fontoura, Ricardo de Albuquerque

; Frizzo, Márcio Leandro dos Santos

; Sá, Enilson Luiz Saccol de

; Osório Filho, Benjamin Dias

; Tonon, Brenda Cristye

; Binz, Andréia

; Silva, Marta Coutinho da

; Camargo, Flavio Anastácio de Oliveira

; Selbach, Pedro Alberto

Ciência Rural

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mar 2011, Volume 41 N. 3 Pages 440 - 446

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

As leguminosas forrageiras, além de contribuírem para o aumento da oferta de forragem com alto teor de proteína, também são importantes como cobertura vegetal de solos. Entre as leguminosas com grande potencial forrageiro, espécies do gênero Lotus têm se destacado. Este trabalho visou à seleção de rizóbios nativos eficientes em Lotus glaber a partir de amostras de solo de cinco localidades do Rio Grande do Sul. Obtiveram-se 259 isolados de rizóbios, que foram avaliados quanto à morfologia colonial e produção de melanina. Destes, 15 isolados foram selecionados para avaliação da eficiência na fixação simbiótica de nitrogênio em experimento em casa de vegetação e caracterizados geneticamente por comparação do perfil eletroforético dos produtos de amplificação do DNA genômico, por PCR com os oligonucleotídeos iniciadores BOX e ERIC. Apenas cinco isolados produziram melanina. Nove isolados foram mais eficientes do que a estirpe SEMIA 830, que é autorizada para a produção de inoculante para L. glaber no país. Na caracterização genética, observou-se que nenhum isolado apresentou identidade com as estirpes recomendadas, o que demonstra que, nos solos do Rio Grande do Sul, existem rizóbios autóctones eficientes na fixação simbiótica de nitrogênio com L. glaber, podendo ser recomendados para estudos a campo, visando a uma futura produção de inoculantes para estas leguminosas no Brasil.

Forage legumes besides contributing to increase the supply of high protein content forage are also good cover for the soil surface. Among the legumes with high potential forage, the genus Lotus has been outstanding. This research aimed to selectnative rhizobia efficient in fixing nitrogen with Lotus glaber, from soil samples of five localities of Rio Grande do Sul. A total of 259 rhizobia isolates were obtained, which were evaluated based on colony morphology and in vitro melanin production. Among them, 15 isolates were selected for the evaluation of the symbiotic nitrogen fixation efficiency in greenhouse experiments and genetically characterized by genomic DNA fingerprinting, PCR amplification with BOX and ERIC primers. Only five isolates produced melanin. Nine isolates were more efficient than strain SEMIA 830, which is authorized for the production of commercial inoculants of L. glaber in Brazil. In relation to the genetic characterization, the isolates have shown no similarity with the commercial strains, indicating that in the soils of Rio Grande do Sul there are autochthonous rhizobia capable of efficient symbiotic nitrogen fixation with L. glaber, which might be recommended for field evaluations, aiming a future production of inoculants for this legume in Brazil.

2672 downloads

10.

II Diretriz de Avaliação Perioperatória da Sociedade Brasileira de Cardiologia
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Feitosa, Alina Coutinho Rodrigues

; Marques, André Coelho

; Caramelli, Bruno

; Ayub, Beatriz

; Polanczyk, Carisi A.

; Jardim, Carlos

; Vieira, Carolina L. Zilli

; Pinho, Claudio

; Calderaro, Daniela

; Gualandro, Danielle Menosi

; Iezzi, Denise

; Ikeoka, Dimas T.

; Schreen, Dirk

; D'Amico, Elbio Antonio

; Pfeferman, Elcio

; Lima, Emerson Quintino de

; Burdmann, Emmanuel de A.

; Pachon, Enrique

; Machado, Fabio Santana

; Galas, Filomena Regina Barbosa Gomes

; Paula, Flávio Jota de

; Carvalho, Francine Corrêa de

; Feitosa-Filho, Gilson Soares

; Prado, Gustavo Faibischew

; Lopes, Heno F.

; Lima, José Jaime Galvão de

; Marchini, Julio Flavio Meirelles

; Fornari, Luciana S.

; Drager, Luciano F.

; Vacanti, Luciano Janussi

; Hajjar, Ludhmila Abrahão

; Rohde, Luis Eduardo P.

; Gowdak, Luís Henrique

; Cardoso, Luiz Francisco

; Vieira, Marcelo Luiz Campos

; Monachini, Maristela C.

; Macatrão, Milena

; Yu, Pai Ching

; Villaça, Paula Ribeiro

; Farsky, Pedro Silvio

; Lopes, Renato Delascio

; Bagnatori, Renato Scotti

; Heinisch, Roberto Henrique

; Gualandro, Sandra F. Menosi

; Accorsi, Tarso Augusto Duenhas

; Ávila, Walkiria Samuel

; Mathias Jr., Wilson

Arquivos Brasileiros de Cardiologia

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2011, Volume 96 N. 3 Pages 1 - 68

Abstract: Pt | Text: Pt | PDF: Pt

53329 downloads

11.

Parentage test in broad-snouted caimans (Caiman latirostris, Crocodylidae) using microsatellite DNA
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Zucoloto, Rodrigo B.

; Verdade, Luciano M.

; Villela, Priscilla M.S.

; Regitano, Luciana C.A.

; Coutinho, Luiz L.

Genetics and Molecular Biology

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2009, Volume 32 N. 4 Pages 874 - 881

Abstract: En | Text: En | PDF: En

In this study, microsatellite markers, developed for Alligator mississipiensis and Caiman latirostris, were used to assess parentage among individuals from the captive colony of Caiman latirostris at the University of São Paulo, in Piracicaba, São Paulo, Brazil. Many of the females in the colony were full siblings, which made maternal identification difficult due to genotypic similarity. Even so, the most likely mother could be identified unambiguously among offspring in most of the clutches studied. Two non-parental females displayed maternal behavior which would have misled managers in assigning maternity based on behavior alone. This set of variable loci demonstrates the utility of parentage testing in captive propagation programs.

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12.

Development of instrumentation for amperometric and coulometric detection using ultramicroelectrodes
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Coutinho, Cláudia F. B.

; Coutinho, Lincoln F. M.

; Lanças, Fernando M.

; Câmara, Carlos A. P.

; Nixdorf, Suzana L.

; Mazo, Luiz H.

Journal of the Brazilian Chemical Society

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2008, Volume 19 N. 1 Pages 131 - 139

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Neste trabalho é apresentado o desenvolvimento de uma instrumentação simples, portátil e de baixo custo para detecção amperométrica e coulométrica usando ultramicroeletrodos em diferentes sistemas analíticos. O programa, desenvolvido em LabVIEW 7.1TM, é capaz de realizar três modos de detecção (amperométrico, coulométrico e amperométrico pulsado) e voltametria cíclica. A instrumentação desenvolvida foi avaliada utilizando os seguintes sistemas: voltametria cíclica de eletrodos metálicos em solução alcalina, detecção eletroquímica em fluxo de glicose e glicina, e também na determinação direta do herbicida glifosato (detecção eletroquímica aliada à cromatografia líquida de alta eficiência, CLAE).

In this work it is presented the development of a simple, portable and inexpensive instrumentation for amperometric and coulometric detection in different analytical instrumentation systems utilizing ultramicroelectrodes. The software, developed in LabVIEW 7.1TM, is capable to carry out three main detection techniques (amperometric, pulsed amperometric and coulometric detection) and a voltammetric technique (cyclic voltammetry). The instrumentation was successfully evaluated using the following systems: cyclic voltammograms of metallic electrodes in alkaline solutions, flow electrochemical detection of glucose and glycine and direct determination of herbicide glyphosate (electrochemical detection coupled to HPLC).

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13.

Leptin and hypothalamic gene expression in early- and late-maturing Bos indicus Nellore heifers
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Vaiciunas, Aline

; Coutinho, Luiz L.

; Meirelles, Flávio V.

; Pires, Alexandre V.

; Silva, Luis Felipe P.

Genetics and Molecular Biology

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2008, Volume 31 N. 3 Pages 657 - 664

Abstract: En | Text: En | PDF: En

We investigated wether early-maturing or late-maturing Bos indicus Nellore heifers produced more leptin mRNA in adipose tissues and altered expression of hypothalamic genes related to leptin signaling. Six prepubertal and six pubertal heifers aged about 34 months and weighing 280 kg to 300 kg each were selected from a population of 100 Nellore heifers. Real-time PCR was used to quantify the expression of the leptin gene (LEP) in adipose tissues and the long isoform of the leptin receptor gene (Ob-Rb), the NK2 homeobox 1 hypothalamic marker gene NKX2-1, the suppressor of cytokine signaling 3 gene (SOCS-3), the neuropeptide Y genes (NPY) and the NPY G-protein coupled receptor genes NPY-Y1 and NPY-Y4 in the hypothalamus. Heifers attaining puberty earlier showed significantly greater LEP expression in adipose tissues (p < 0.05) and there was tissue interaction (p < 0.05). Hypothalamic expression of Ob-Rb, NKX2-1, NPY and SOCS-3 did not differ between groups, but in early-maturing heifers there was a tendency for lower expression of NPY-Y1 (8.3-fold less) and NPY-Y4 (14.3-fold less) compared to late-maturing heifers (p = 0.1). These results suggest that a combination of higher LEP expression, lower NPY-Y1 and NPY-Y4 expression could be a factor in regulating puberty in early-maturing B. indicus heifers.

2486 downloads Cited 2 times in SciELO

14.

Plant compounds insecticide activity against Coleoptera pests of stored products
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Moreira, Márcio Dionizio

; Picanço, Marcelo Coutinho

; Barbosa, Luiz Cláudio de Almeida

; Guedes, Raul Narciso Carvalho

; Campos, Mateus Ribeiro de

; Silva, Gerson Adriano

; Martins, Júlio Cláudio

Pesquisa Agropecuária Brasileira

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jul 2007, Volume 42 N. 7 Pages 909 - 915

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

O objetivo deste trabalho foi selecionar plantas com atividade inseticida, para isolar, identificar e avaliar a bioatividade de compostos inseticidas presentes nessas plantas, contra as seguintes pragas de produtos armazenados da ordem Coleoptera: Sitophilus zeamais Mots. (Curculionidae), Rhyzopertha dominica F. (Bostrichidae) e Oryzaephilus surinamensis L. (Silvanidae). As espécies de plantas usadas foram: anis (Ocimum selloi Benth), arruda (Ruta graveolens L.), cordão-de-frade (Leonotis nepetifolia L.), datura (Datura stramonium L.), erva baleeira (Cordia verbenacea L.), hortelã (Mentha piperita L.), melão-de-são-caetano (Mormodica charantia L.) e mentrasto (Ageratum conyzoides L.). Avaliou-se a toxicidade dos extratos hexânico e etanólico dessas plantas sobre R. dominica. Somente o extrato hexânico de A. conyzoides apresentou atividade inseticida. O extrato hexânico desta planta foi fracionado, sucessivamente, por cromatografia de coluna de sílica gel, para isolamento e purificação dos compostos ativos. Os compostos 5,6,7,8,3',4',5'-heptametoxiflavona; 5,6,7,8,3'-pentametoxi-4',5'-metilenodioxiflavona e cumarina foram identificados. Entretanto, somente a cumarina apresentou atividade inseticida às três espécies de insetos (DL50 de 2,72 a 39,71 mg g-1 de i.a.). A ordem crescente de suscetibilidade à cumarina foi R. dominica, S. zeamais e O. surinamensis.

The objective of this work was to screen plants with insecticide activity, in order to isolate, identify and assess the bioactivity of insecticide compounds present in these plants, against Coleoptera pests of stored products: Oryzaephilus surinamensis L. (Silvanidae), Rhyzopertha dominica F. (Bostrichidae) and Sitophilus zeamais Mots. (Curculionidae). The plant species used were: basil (Ocimum selloi Benth.), rue (Ruta graveolens L.), lion's ear (Leonotis nepetifolia (L.) R.Br.), jimson weed (Datura stramonium L.), baleeira herb (Cordia verbenacea L.), mint (Mentha piperita L.), wild balsam apple (Mormodica charantia L.), and billy goat weed or mentrasto (Ageratum conyzoides L.). The insecticide activity of hexane and ethanol extracts from those plants on R. dominica was evaluated. Among them, only hexane extract of A. conyzoides showed insecticide activity; the hexane extract of this species was successively fractionated by silica gel column chromatography, for isolation and purification of the active compounds. Compounds 5,6,7,8,3',4',5'-heptamethoxyflavone; 5,6,7,8,3'-pentamethoxy-4',5'-methilenedioxyflavone and coumarin were identified. However, only coumarin showed insecticide activity against three insect pests (LD50 from 2.72 to 39.71 mg g-1 a.i.). The increasing order of insects susceptibility to coumarin was R. dominica, S. zeamais and O. surinamensis.

20921 downloads Cited 3 times in SciELO

15.

Effect of growth hormone on fatty acid synthase gene expression in porcine adipose tissue cultures
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José, Andrea A.F.B.V.

; Alvares, Lúcia. E.

; Delgado, Eduardo F.

; Coutinho, Luiz L.

; Lanna, Dante P.D.

Genetics and Molecular Biology

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2006, Volume 29 N. 1 Pages 180 - 186

Abstract: En | Text: En | PDF: En

We describe an efficient in vitro assay to test growth hormone effects on mRNA levels and fatty acid synthase (FAS, EC. 2.3.1.85) activity. Swine adipose tissue explants were long-term cultured in medium containing growth hormone and FAS mRNA levels and enzyme activity were measured. We quantified FAS transcripts by competitive reverse transcriptase PCR (RT-PCR) using total RNA from cultured adipose tissue explants and RT-PCR standard-curves were constructed using a cloned 307 bp segment of native FAS cDNA and a shorter fragment from which a 64 bp (competitor, 243 bp) internal sequence had been deleted. A known amount of competitor was added to each PCR as an internal control and µ-actin transcripts were also measured to correct for differences in total RNA extraction and reverse transcription efficiency. In cultures with added growth hormone FAS mRNA levels decreased 70% (p < 0.01) and FAS enzyme activity decreased 22% (p < 0.05). These in vitro growth hormone effects were consistent with those observed in vivo, showing that in vitro adipose tissue culture combined with RT-PCR is a useful and accurate tool for studying growth hormone modulation of adipose tissue metabolism. This technique allowed the diagnosis of lower levels of FAS mRNA in the presence of growth hormone and these low levels were associated with decreased FAS activity in the adipose tissue explants.

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