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au:Costa, Maria C. N.
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1.
Chemical and dereplication studies of Palicourea tomentosa (Aubl.) Borhidi and their antimicrobial and anticholinesterase activities Aubl. Aubl (Aubl. (Aubl
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Cecatto, Ana J.
; Formagio, Anelise S. N.
; Buzanello-Martins, Cleide V.
; Fortuna, Caroline
; Cabral, Márcia R. P.
; Costa, Willian F. da
; Baldoqui, Débora C.
; Sarragiotto, Maria H.
.
Palicourea tomentosa (Aubl.) Borhidi (Rubiaceae, Palicoureeae), earlier classified as Psychotria poeppigiana Müll. Arg., is used in folk medicine for a variety of diseases. Biological studies on the plant describes their vasorelaxant, antiplasmodial, antitumoural and anticholinesterase activities. To expand the knowledge on the chemical and biological potential of this species, in this work, phytochemical and dereplication studies, as well as antimicrobial and anticholinesterase evaluation of Palicourea tomentosa were carried out. Phytochemical investigation deals with the isolation of lutein (1), a mixture of dehydrovomifoliol (2) and megastigma-4,7-dien-3-one (3), loliolide (4), 5,13-epoxy-9-hydroxy-megastigma-7-en,3-one (5), vomifoliol (6), asperuloside (7), 3-O-p-coumaroylquinic acid (8), and a mixture of the pyrrolizidinoindoline alkaloids hodgkinsine (9), chimonanthine (10) and psychotriasine (11). The ultra-high performance liquid chromatography coupled to high-resolution mass spectrometry (UHPLC HRMS/MS) based on molecular networking dereplication of dichloromethane fraction led to putative identification of other two megastigmanes, one terpene lactone and three carotenoids, besides seven triterpenes and four indole alkaloids. The dichloromethane, ethyl acetate and hydromethanol fractions of P. tomentosa were able to inhibit Candida albicans with minimum inhibitory concentrations (MICs) of 125, 250 and 125 µg mL-1, respectively. The methanol crude extract, dichloromethane and alkaloid fractions inhibited selectively the butyrilcholinesterase (BuChE) at percentages of 63.09, 60.39 and 56.22%, respectively. Aubl. Aubl (Aubl. Rubiaceae, Rubiaceae (Rubiaceae Palicoureeae, Palicoureeae , Palicoureeae) Müll Arg Arg. diseases vasorelaxant antiplasmodial activities species work out 1, 1 (1) 2 (2 megastigma4,7dien3one megastigma47dien3one megastigmadienone megastigma 4,7 dien 3 4 7 3, (3) 4, (4) 5,13epoxy9hydroxymegastigma7en,3one 513epoxy9hydroxymegastigma7en3one epoxyhydroxymegastigmaenone 5,13 epoxy 9 hydroxy en,3 5 13 en 5, (5) 6, 6 (6) 7, (7) 3Opcoumaroylquinic Opcoumaroylquinic O p coumaroylquinic 8, 8 (8) 9, (9) 10 (10 11. 11 . (11) ultrahigh ultra high highresolution resolution UHPLC HRMS/MS HRMSMS HRMS MS megastigmanes carotenoids P MICs (MICs 25 12 mL1, mL1 mL mL-1 respectively extract BuChE (BuChE 6309 63 09 63.09 6039 60 39 60.3 5622 56 22 56.22% (Aubl (1 ( megastigma4 7dien3one 47 (3 (4 13epoxy9hydroxymegastigma7en 3one hydroxymegastigma 513 5,1 en3 en, (5 (6 (7 (8 (9 (11 mL- 630 0 63.0 603 60. 562 56.22 dienone epoxyhydroxymegastigmaen 51 63. 56.2 56.
2.
Synthesis, in vitro Toxicity, and Antitrypanosomal Activity of Arylated and Diarylated Thiazoles Synthesis Toxicity
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Figueira, Kelly L.
; Girão, Roberson D.
; Costa, Krislayne N. da
; Barreto, Ana C. R.
; Soeiro, Maria de Nazaré C.
; Limberger, Jones
.
Chagas disease is a relevant public health threat that affects over 6 million people worldwide, resulting in devastating social and economic consequences. Moreover, the therapeutic options are limited, highlighting the urgency in searching for novel active antitrypanosomal molecules. Compounds with either thiazole or biaryl units have been described as possessing anti Trypanosoma cruzi activities. Therefore, here, we describe the synthesis of nine arylated and diarylated thiazole derivatives and the evaluation of their in vitro toxicity on mammalian cells as well as their anti T. cruzi activity. The compounds were prepared in straightforward synthetic routes, using Hantzsch thiazole synthesis and cross-coupling reactions as key steps. A pyridyl-phenyl-thiazole (PPT) derivative (4c) presented 76% of T. cruzi growth inhibition in preliminary tests using a fixed concentration of 20 µM. This compound was used as a scaffold for the synthesis of two novel PPT analogs (4g and 4h). Dose-response assays on intracellular forms of T. cruzi demonstrated that these three compounds presented high antiparasitic potency (half maximal effective concentration (EC50) values ranging from 1.15 to 2.38 μM) and low toxic profile against L929 cell lines. Hence, these findings highlight the pyridyl-phenyl-thiazole backbone as a novel privileged scaffold in the search for active molecules against T. cruzi. worldwide consequences Moreover limited activities Therefore here T activity routes crosscoupling cross coupling steps pyridylphenylthiazole pyridyl phenyl (PPT 4c c (4c 76 2 µM 4g g 4h. 4h h . 4h) Doseresponse Dose response half EC50 EC (EC50 115 1 15 1.1 238 38 2.3 μM L L92 lines Hence 7 EC5 (EC5 11 1. 23 3 2. L9 (EC
3.
Patients hospitalized with active tuberculosis and Covid-19 coinfection: A matched case-control from the Brazilian Covid-19 Registry Covid19 Covid 19 Covid-1 coinfection casecontrol case control Covid1 1 Covid-
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CARVALHO, RAFAEL L.R.
; AGUIAR, GABRIELLA G.
; MOREIRA, JESSICA F.B.
; PEREIRA, DANIELLA N.
; AUGUSTO, VALÉRIA MARIA
; SCHWARZBOLD, ALEXANDRE V.
; MATOS, CAROLINA C.
; RIOS, DANYELLE R.A.
; COSTA, FELÍCIO R.
; ANSCHAU, FERNANDO
; CHATKIN, JOSÉ MIGUEL
; RUSCHEL, KAREN B.
; CARNEIRO, MARCELO
; OLIVEIRA, NEIMY R. DE
; PARAÍSO, PEDRO G.
; AGUIAR, RUBIA LAURA O.
; GRIZENDE, GENNA MAIRA S.
; MARCOLINO, MILENA S.
.
Abstract Although control of Covid-19 has improved, the virus continues to cause infections, such as tuberculosis, that is still endemic in many countries, representing a scenario of coinfection. To compare Covid-19 clinical manifestations and outcomes between patients with active tuberculosis infection and matched controls. This is a matched case-control study based on data from the Brazilian Covid-19 Registry, in hospitalized patients aged 18 or over with laboratory confirmed Covid-19 from March 1, 2020, to March 31, 2022. Cases were patients with tuberculosis and controls were Covid-19 patients without tuberculosis. From 13,636 Covid-19, 36 also had active tuberculosis (0.0026%). Pulmonary fibrosis (5.6% vs 0.0%), illicit drug abuse (30.6% vs 3.0%), alcoholism (33.3% vs 11.9%) and smoking (50.0% vs 9.7%) were more common among patients with tuberculosis. They also had a higher frequency of nausea and vomiting (25.0% vs 10.4%). There were no significant differences in in-hospital mortality, mechanical ventilation, need for dialysis and ICU stay. Patients with TB infection presented a higher frequency of pulmonary fibrosis, abuse of illicit drugs, alcoholism, current smoking, symptoms of nausea and vomiting. The outcomes were similar between them. Covid19 Covid 19 Covid-1 improved infections countries coinfection casecontrol case Registry 1 2020 31 2022 13636 13 636 13,63 Covid19, 19, 3 0.0026%. 00026 0.0026% . 0 0026 (0.0026%) 5.6% 56 5 6 (5.6 0.0%, 00 0.0% , 0.0%) 30.6% 306 30 (30.6 3.0%, 3.0% 3.0%) 33.3% 333 33 (33.3 11.9% 119 11 9 50.0% 500 50 (50.0 9.7% 97 7 25.0% 250 25 (25.0 10.4%. 104 10.4% 10 4 10.4%) inhospital hospital mortality ventilation stay drugs them Covid1 Covid- 202 1363 63 13,6 0002 0.0026 002 (0.0026% 5.6 (5. 0.0 30.6 (30. 3.0 33.3 (33. 11.9 50.0 (50. 9.7 25.0 2 (25. 10.4 20 136 13, 000 0.002 (0.0026 5. (5 0. 30. (30 3. 33. (33 11. 50. (50 9. 25. (25 10. 0.00 (0.002 ( (3 (2 (0.00 (0.0 (0. (0
4.
Smart Solid Drug Delivery Systems: Exploring the pH Sensitivity of Cashew Gum-Doxorubicin Prodrug Systems GumDoxorubicin Gum Doxorubicin
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Ribeiro, Irisvan S.
; Leonardo, Adisom L. S.
; Carneiro, Maria J. M.
; Costa Filho, Raimundo N.
; Freire, Rosemayre S.
; Sousa, Jeanlex S.
; Araújo, Gisele S.
; Marinho Filho, José D. B.
; Araújo, Ana J.
; Paula, Haroldo C. B.
; Feitosa, Judith P. A.
; Paula, Regina C M. de
.
Journal of the Brazilian Chemical Society
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Polysaccharide-based prodrugs formed via pH-responsive covalent interactions can be used to prepare nanoparticles for drug delivery to tumor cells. This study reports the synthesis of cashew gum-doxorubicin prodrugs via Schiff base (CG-S-DOX) and amide bonds (CG-A-DOX), both of which exhibit pH-responsive behavior. Synthesis was confirmed using spectroscopic techniques. Drug-binding content and efficiency were higher for CG-S-DOX than for CG-A-DOX. The capacity of the nanoparticles to self-organize in aqueous media was confirmed using fluorescence spectroscopy, dynamic light scattering, and atomic force microscopy. Both prodrugs possessed sizes < 200 nm and showed responsive doxorubicin-release profiles in the acidic tumor cell microenvironment. Compared with free DOX, the CG-S-DOX and CG-A-DOX prodrug had significantly reduced cytotoxicity against non-tumor cells (L929). CG-S-DOX, but not CG-A-DOX, showed antitumor activity against HCT-116 (human colorectal cancer) and MCF-7 (human breast cancer) cells. An uptake assay confirmed that the nanoparticles were easily taken up by HCT-116 cells. These results together with the great reduction in cytotoxicity against non-tumor cells, confirm the potential of CG-S-DOX prodrug nanoparticles as a reliable and efficient system for the effective delivery of doxorubicin to tumor cells. Polysaccharidebased Polysaccharide based pHresponsive pH gumdoxorubicin gum CGSDOX CG S DOX (CG-S-DOX CGADOX, CGADOX A , (CG-A-DOX) behavior techniques Drugbinding Drug binding CGADOX. DOX. selforganize self organize spectroscopy scattering microscopy 20 doxorubicinrelease release microenvironment nontumor non L929. L929 L . (L929) CGSDOX, HCT116 HCT 116 HCT-11 human cancer MCF7 MCF 7 MCF- (CG-A-DOX 2 L92 (L929 HCT11 11 HCT-1 L9 (L92 HCT1 1 HCT- (L9 (L
5.
Production and quality of pak choi grown in different hydroponic systems and electrical conductivities
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Oliveira, Francisco de A. de
; Costa, Mário J. V.
; Oliveira, Maria do C. de
; Oliveira, Mychelle K. T. de
; Silva, Ronimeire T. da
; Góis, Helena M. de M. N.
; Ribeiro Filho, Jacques C.
.
RESUMO O pak choi é uma hortaliça folhosa rica bioativos importantes para a saúde humana. Por ser cultivado predominantemente nos continentes europeu e asiático, existem poucas informações sobre esta hortaliça na literatura brasileira. Objetivou-se avaliar a produção e a qualidade do pak choi cultivado em sistemas hidropônicos utilizando soluções nutritivas de diferentes valores de condutividade elétrica. Para isto, o experimento foi instalado seguindo o delineamento em blocos casualizados, em esquema fatorial 2 × 5, sendo dois sistemas hidropônicos (NFT e Semi-hidropônico) e cinco níveis de condutividade elétrica (1,0; 2,0; 3,0; 4,0 e 5,0 dS m-1). As plantas foram colhidas aos 35 dias após o transplantio e avaliadas quanto às seguintes variáveis: altura de planta, número de folhas, diâmetro do caule, área foliar, massa fresca e seca da parte aérea, suculência foliar, área foliar específica, firmeza do pecíolo, pH do suco e sólidos solúveis. As variáveis de crescimento e qualidade foram afetadas pela interação entre os fatores CEs e sistemas hidropônicos. O sistema semi-hidropônico proporcionou o maior desenvolvimento das plantas e maior tolerância à salinidade. As plantas cultivadas no sistema NFT apresentaram menor exigência nutricional para atingir o máximo crescimento. O aumento da CE da solução nutritiva não afetou a qualidade visual do pak choi, mas reduziu a qualidade físico-química das folhas. humana asiático brasileira Objetivouse Objetivou se isto casualizados 5 Semihidropônico Semi hidropônico Semi-hidropônico 1,0 10 1 0 (1,0 2,0 20 3,0 30 3 40 4 4, 50 m1. m1 m . m-1) planta folhas caule aérea específica pecíolo solúveis semihidropônico semi salinidade físicoquímica físico química 1, (1, 2, 3, m-1 (1 m- (
ABSTRACT Pak choi is a leafy vegetable rich in important bioactives for human health. As this vegetable is cultivated predominantly in Europe and Asia, there is little information about it in the Brazilian literature. The aim of this study was to evaluate the production and quality of pak choi cultivated in hydroponic systems using nutrient solutions of different electrical conductivities. An experiment was set up following a randomized block design, in a 2 × 5 factorial scheme, with two hydroponic systems (NFT and Semi-hydroponic) and five levels of electrical conductivity (1.0, 2.0, 3.0, 4.0 and 5.0 dS m-1). The plants were harvested 35 days after transplanting and evaluated for the following variables: plant height, number of leaves, stem diameter, leaf area, shoot fresh and dry mass, leaf succulence, specific leaf area, petiole firmness, juice pH and soluble solids. Growth and quality variables were affected by the interaction between EC levels and hydroponic systems. The semi-hydroponic system promoted the greatest development of pak choi plants and greater tolerance to salinity. Plants cultivated in the NFT system had a lower nutritional requirement to reach maximum growth. Increase in nutrient solution EC did not affect the visual quality of pak choi, but reduced the physicochemical quality of its leaves. health Asia literature conductivities design scheme Semihydroponic Semi Semi-hydroponic 1.0, 10 1 0 (1.0 20 2.0 30 3 3.0 40 4 4. 50 5. m1. m1 m . m-1) height leaves diameter area mass succulence firmness solids semihydroponic semi salinity growth 1.0 (1. 2. 3. m-1 1. (1 m- (
6.
Catálogo Taxonômico da Fauna do Brasil: Setting the baseline knowledge on the animal diversity in Brazil Brasil
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Boeger, Walter A.
; Valim, Michel P.
; Zaher, Hussam
; Rafael, José A.
; Forzza, Rafaela C.
; Percequillo, Alexandre R.
; Serejo, Cristiana S.
; Garraffoni, André R.S.
; Santos, Adalberto J.
; Slipinski, Adam
; Linzmeier, Adelita M.
; Calor, Adolfo R.
; Garda, Adrian A.
; Kury, Adriano B.
; Fernandes, Agatha C.S.
; Agudo-Padrón, Aisur I.
; Akama, Alberto
; Silva Neto, Alberto M. da
; Burbano, Alejandro L.
; Menezes, Aleksandra
; Pereira-Colavite, Alessandre
; Anichtchenko, Alexander
; Lees, Alexander C.
; Bezerra, Alexandra M.R.
; Domahovski, Alexandre C.
; Pimenta, Alexandre D.
; Aleixo, Alexandre L.P.
; Marceniuk, Alexandre P.
; Paula, Alexandre S. de
; Somavilla, Alexandre
; Specht, Alexandre
; Camargo, Alexssandro
; Newton, Alfred F.
; Silva, Aline A.S. da
; Santos, Aline B. dos
; Tassi, Aline D.
; Aragão, Allan C.
; Santos, Allan P.M.
; Migotto, Alvaro E.
; Mendes, Amanda C.
; Cunha, Amanda
; Chagas Júnior, Amazonas
; Sousa, Ana A.T. de
; Pavan, Ana C.
; Almeida, Ana C.S.
; Peronti, Ana L.B.G.
; Henriques-Oliveira, Ana L.
; Prudente, Ana L.
; Tourinho, Ana L.
; Pes, Ana M.O.
; Carmignotto, Ana P.
; Wengrat, Ana P.G. da Silva
; Dornellas, Ana P.S.
; Molin, Anamaria Dal
; Puker, Anderson
; Morandini, André C.
; Ferreira, André da S.
; Martins, André L.
; Esteves, André M.
; Fernandes, André S.
; Roza, André S.
; Köhler, Andreas
; Paladini, Andressa
; Andrade, Andrey J. de
; Pinto, Ângelo P.
; Salles, Anna C. de A.
; Gondim, Anne I.
; Amaral, Antonia C.Z.
; Rondón, Antonio A.A.
; Brescovit, Antonio
; Lofego, Antônio C.
; Marques, Antonio C.
; Macedo, Antonio
; Andriolo, Artur
; Henriques, Augusto L.
; Ferreira Júnior, Augusto L.
; Lima, Aurino F. de
; Barros, Ávyla R. de A.
; Brito, Ayrton do R.
; Romera, Bárbara L.V.
; Vasconcelos, Beatriz M.C. de
; Frable, Benjamin W.
; Santos, Bernardo F.
; Ferraz, Bernardo R.
; Rosa, Brunno B.
; Sampaio, Brunno H.L.
; Bellini, Bruno C.
; Clarkson, Bruno
; Oliveira, Bruno G. de
; Corrêa, Caio C.D.
; Martins, Caleb C.
; Castro-Guedes, Camila F. de
; Souto, Camilla
; Bicho, Carla de L.
; Cunha, Carlo M.
; Barboza, Carlos A. de M.
; Lucena, Carlos A.S. de
; Barreto, Carlos
; Santana, Carlos D.C.M. de
; Agne, Carlos E.Q.
; Mielke, Carlos G.C.
; Caetano, Carlos H.S.
; Flechtmann, Carlos H.W.
; Lamas, Carlos J.E.
; Rocha, Carlos
; Mascarenhas, Carolina S.
; Margaría, Cecilia B.
; Waichert, Cecilia
; Digiani, Celina
; Haddad, Célio F.B.
; Azevedo, Celso O.
; Benetti, Cesar J.
; Santos, Charles M.D. dos
; Bartlett, Charles R.
; Bonvicino, Cibele
; Ribeiro-Costa, Cibele S.
; Santos, Cinthya S.G.
; Justino, Cíntia E.L.
; Canedo, Clarissa
; Bonecker, Claudia C.
; Santos, Cláudia P.
; Carvalho, Claudio J.B. de
; Gonçalves, Clayton C.
; Galvão, Cleber
; Costa, Cleide
; Oliveira, Cléo D.C. de
; Schwertner, Cristiano F.
; Andrade, Cristiano L.
; Pereira, Cristiano M.
; Sampaio, Cristiano
; Dias, Cristina de O.
; Lucena, Daercio A. de A.
; Manfio, Daiara
; Amorim, Dalton de S.
; Queiroz, Dalva L. de
; Queiroz, Dalva L. de
; Colpani, Daniara
; Abbate, Daniel
; Aquino, Daniel A.
; Burckhardt, Daniel
; Cavallari, Daniel C.
; Prado, Daniel de C. Schelesky
; Praciano, Daniel L.
; Basílio, Daniel S.
; Bená, Daniela de C.
; Toledo, Daniela G.P. de
; Takiya, Daniela M.
; Fernandes, Daniell R.R.
; Ament, Danilo C.
; Cordeiro, Danilo P.
; Silva, Darliane E.
; Pollock, Darren A.
; Muniz, David B.
; Gibson, David I.
; Nogueira, David S.
; Marques, Dayse W.A.
; Lucatelli, Débora
; Garcia, Deivys M.A.
; Baêta, Délio
; Ferreira, Denise N.M.
; Rueda-Ramírez, Diana
; Fachin, Diego A.
; Souza, Diego de S.
; Rodrigues, Diego F.
; Pádua, Diego G. de
; Barbosa, Diego N.
; Dolibaina, Diego R.
; Amaral, Diogo C.
; Chandler, Donald S.
; Maccagnan, Douglas H.B.
; Caron, Edilson
; Carvalho, Edrielly
; Adriano, Edson A.
; Abreu Júnior, Edson F. de
; Pereira, Edson H.L.
; Viegas, Eduarda F.G.
; Carneiro, Eduardo
; Colley, Eduardo
; Eizirik, Eduardo
; Santos, Eduardo F. dos
; Shimbori, Eduardo M.
; Suárez-Morales, Eduardo
; Arruda, Eliane P. de
; Chiquito, Elisandra A.
; Lima, Élison F.B.
; Castro, Elizeu B. de
; Orlandin, Elton
; Nascimento, Elynton A. do
; Razzolini, Emanuel
; Gama, Emanuel R.R.
; Araujo, Enilma M. de
; Nishiyama, Eric Y.
; Spiessberger, Erich L.
; Santos, Érika C.L. dos
; Contreras, Eugenia F.
; Galati, Eunice A.B.
; Oliveira Junior, Evaldo C. de
; Gallardo, Fabiana
; Hernandes, Fabio A.
; Lansac-Tôha, Fábio A.
; Pitombo, Fabio B.
; Dario, Fabio Di
; Santos, Fábio L. dos
; Mauro, Fabio
; Nascimento, Fabio O. do
; Olmos, Fabio
; Amaral, Fabio R.
; Schunck, Fabio
; Godoi, Fábio S. P. de
; Machado, Fabrizio M.
; Barbo, Fausto E.
; Agrain, Federico A.
; Ribeiro, Felipe B.
; Moreira, Felipe F.F.
; Barbosa, Felipe F.
; Silva, Fenanda S.
; Cavalcanti, Fernanda F.
; Straube, Fernando C.
; Carbayo, Fernando
; Carvalho Filho, Fernando
; Zanella, Fernando C.V.
; Jacinavicius, Fernando de C.
; Farache, Fernando H.A.
; Leivas, Fernando
; Dias, Fernando M.S.
; Mantellato, Fernando
; Vaz-de-Mello, Fernando Z.
; Gudin, Filipe M.
; Albuquerque, Flávio
; Molina, Flavio B.
; Passos, Flávio D.
; Shockley, Floyd W.
; Pinheiro, Francielly F.
; Mello, Francisco de A.G. de
; Nascimento, Francisco E. de L.
; Franco, Francisco L.
; Oliveira, Francisco L. de
; Melo, Francisco T. de V.
; Quijano, Freddy R.B.
; Salles, Frederico F.
; Biffi, Gabriel
; Queiroz, Gabriel C.
; Bizarro, Gabriel L.
; Hrycyna, Gabriela
; Leviski, Gabriela
; Powell, Gareth S.
; Santos, Geane B. dos
; Morse, Geoffrey E.
; Brown, George
; Mattox, George M.T.
; Zimbrão, Geraldo
; Carvalho, Gervásio S.
; Miranda, Gil F.G.
; Moraes, Gilberto J. de
; Lourido, Gilcélia M.
; Neves, Gilmar P.
; Moreira, Gilson R.P.
; Montingelli, Giovanna G.
; Maurício, Giovanni N.
; Marconato, Gláucia
; Lopez, Guilherme E.L.
; Silva, Guilherme L. da
; Muricy, Guilherme
; Brito, Guilherme R.R.
; Garbino, Guilherme S.T.
; Flores, Gustavo E.
; Graciolli, Gustavo
; Libardi, Gustavo S.
; Proctor, Heather C.
; Gil-Santana, Helcio R.
; Varella, Henrique R.
; Escalona, Hermes E.
; Schmitz, Hermes J.
; Rodrigues, Higor D.D.
; Galvão Filho, Hilton de C.
; Quintino, Hingrid Y.S.
; Pinto, Hudson A.
; Rainho, Hugo L.
; Miyahira, Igor C.
; Gonçalves, Igor de S.
; Martins, Inês X.
; Cardoso, Irene A.
; Oliveira, Ismael B. de
; Franz, Ismael
; Fernandes, Itanna O.
; Golfetti, Ivan F.
; S. Campos-Filho, Ivanklin
; Oliveira, Ivo de S.
; Delabie, Jacques H.C.
; Oliveira, Jader de
; Prando, Jadila S.
; Patton, James L.
; Bitencourt, Jamille de A.
; Silva, Janaina M.
; Santos, Jandir C.
; Arruda, Janine O.
; Valderrama, Jefferson S.
; Dalapicolla, Jeronymo
; Oliveira, Jéssica P.
; Hájek, Jiri
; Morselli, João P.
; Narita, João P.
; Martin, João P.I.
; Grazia, Jocélia
; McHugh, Joe
; Cherem, Jorge J.
; Farias Júnior, José A.S.
; Fernandes, Jose A.M.
; Pacheco, José F.
; Birindelli, José L.O.
; Rezende, José M.
; Avendaño, Jose M.
; Duarte, José M. Barbanti
; Ribeiro, José R. Inácio
; Mermudes, José R.M.
; Pujol-Luz, José R.
; Santos, Josenilson R. dos
; Câmara, Josenir T.
; Teixeira, Joyce A.
; Prado, Joyce R. do
; Botero, Juan P.
; Almeida, Julia C.
; Kohler, Julia
; Gonçalves, Julia P.
; Beneti, Julia S.
; Donahue, Julian P.
; Alvim, Juliana
; Almeida, Juliana C.
; Segadilha, Juliana L.
; Wingert, Juliana M.
; Barbosa, Julianna F.
; Ferrer, Juliano
; Santos, Juliano F. dos
; Kuabara, Kamila M.D.
; Nascimento, Karine B.
; Schoeninger, Karine
; Campião, Karla M.
; Soares, Karla
; Zilch, Kássia
; Barão, Kim R.
; Teixeira, Larissa
; Sousa, Laura D. do N.M. de
; Dumas, Leandro L.
; Vieira, Leandro M.
; Azevedo, Leonardo H.G.
; Carvalho, Leonardo S.
; Souza, Leonardo S. de
; Rocha, Leonardo S.G.
; Bernardi, Leopoldo F.O.
; Vieira, Letícia M.
; Johann, Liana
; Salvatierra, Lidianne
; Oliveira, Livia de M.
; Loureiro, Lourdes M.A. El-moor
; Barreto, Luana B.
; Barros, Luana M.
; Lecci, Lucas
; Camargos, Lucas M. de
; Lima, Lucas R.C.
; Almeida, Lucia M.
; Martins, Luciana R.
; Marinoni, Luciane
; Moura, Luciano de A.
; Lima, Luciano
; Naka, Luciano N.
; Miranda, Lucília S.
; Salik, Lucy M.
; Bezerra, Luis E.A.
; Silveira, Luis F.
; Campos, Luiz A.
; Castro, Luiz A.S. de
; Pinho, Luiz C.
; Silveira, Luiz F.L.
; Iniesta, Luiz F.M.
; Tencatt, Luiz F.C.
; Simone, Luiz R.L.
; Malabarba, Luiz R.
; Cruz, Luiza S. da
; Sekerka, Lukas
; Barros, Lurdiana D.
; Santos, Luziany Q.
; Skoracki, Maciej
; Correia, Maira A.
; Uchoa, Manoel A.
; Andrade, Manuella F.G.
; Hermes, Marcel G.
; Miranda, Marcel S.
; Araújo, Marcel S. de
; Monné, Marcela L.
; Labruna, Marcelo B.
; Santis, Marcelo D. de
; Duarte, Marcelo
; Knoff, Marcelo
; Nogueira, Marcelo
; Britto, Marcelo R. de
; Melo, Marcelo R.S. de
; Carvalho, Marcelo R. de
; Tavares, Marcelo T.
; Kitahara, Marcelo V.
; Justo, Marcia C.N.
; Botelho, Marcia J.C.
; Couri, Márcia S.
; Borges-Martins, Márcio
; Felix, Márcio
; Oliveira, Marcio L. de
; Bologna, Marco A.
; Gottschalk, Marco S.
; Tavares, Marcos D.S.
; Lhano, Marcos G.
; Bevilaqua, Marcus
; Santos, Marcus T.T.
; Domingues, Marcus V.
; Sallum, Maria A.M.
; Digiani, María C.
; Santarém, Maria C.A.
; Nascimento, Maria C. do
; Becerril, María de los A.M.
; Santos, Maria E.A. dos
; Passos, Maria I. da S. dos
; Felippe-Bauer, Maria L.
; Cherman, Mariana A.
; Terossi, Mariana
; Bartz, Marie L.C.
; Barbosa, Marina F. de C.
; Loeb, Marina V.
; Cohn-Haft, Mario
; Cupello, Mario
; Martins, Marlúcia B.
; Christofersen, Martin L.
; Bento, Matheus
; Rocha, Matheus dos S.
; Martins, Maurício L.
; Segura, Melissa O.
; Cardenas, Melissa Q.
; Duarte, Mércia E.
; Ivie, Michael A.
; Mincarone, Michael M.
; Borges, Michela
; Monné, Miguel A.
; Casagrande, Mirna M.
; Fernandez, Monica A.
; Piovesan, Mônica
; Menezes, Naércio A.
; Benaim, Natalia P.
; Reategui, Natália S.
; Pedro, Natan C.
; Pecly, Nathalia H.
; Ferreira Júnior, Nelson
; Silva Júnior, Nelson J. da
; Perioto, Nelson W.
; Hamada, Neusa
; Degallier, Nicolas
; Chao, Ning L.
; Ferla, Noeli J.
; Mielke, Olaf H.H.
; Evangelista, Olivia
; Shibatta, Oscar A.
; Oliveira, Otto M.P.
; Albornoz, Pablo C.L.
; Dellapé, Pablo M.
; Gonçalves, Pablo R.
; Shimabukuro, Paloma H.F.
; Grossi, Paschoal
; Rodrigues, Patrícia E. da S.
; Lima, Patricia O.V.
; Velazco, Paul
; Santos, Paula B. dos
; Araújo, Paula B.
; Silva, Paula K.R.
; Riccardi, Paula R.
; Garcia, Paulo C. de A.
; Passos, Paulo G.H.
; Corgosinho, Paulo H.C.
; Lucinda, Paulo
; Costa, Paulo M.S.
; Alves, Paulo P.
; Roth, Paulo R. de O.
; Coelho, Paulo R.S.
; Duarte, Paulo R.M.
; Carvalho, Pedro F. de
; Gnaspini, Pedro
; Souza-Dias, Pedro G.B.
; Linardi, Pedro M.
; Bartholomay, Pedro R.
; Demite, Peterson R.
; Bulirsch, Petr
; Boll, Piter K.
; Pereira, Rachel M.M.
; Silva, Rafael A.P.F.
; Moura, Rafael B. de
; Boldrini, Rafael
; Silva, Rafaela A. da
; Falaschi, Rafaela L.
; Cordeiro, Ralf T.S.
; Mello, Ramon J.C.L.
; Singer, Randal A.
; Querino, Ranyse B.
; Heleodoro, Raphael A.
; Castilho, Raphael de C.
; Constantino, Reginaldo
; Guedes, Reinaldo C.
; Carrenho, Renan
; Gomes, Renata S.
; Gregorin, Renato
; Machado, Renato J.P.
; Bérnils, Renato S.
; Capellari, Renato S.
; Silva, Ricardo B.
; Kawada, Ricardo
; Dias, Ricardo M.
; Siewert, Ricardo
; Brugnera, Ricaro
; Leschen, Richard A.B.
; Constantin, Robert
; Robbins, Robert
; Pinto, Roberta R.
; Reis, Roberto E. dos
; Ramos, Robson T. da C.
; Cavichioli, Rodney R.
; Barros, Rodolfo C. de
; Caires, Rodrigo A.
; Salvador, Rodrigo B.
; Marques, Rodrigo C.
; Araújo, Rodrigo C.
; Araujo, Rodrigo de O.
; Dios, Rodrigo de V.P.
; Johnsson, Rodrigo
; Feitosa, Rodrigo M.
; Hutchings, Roger W.
; Lara, Rogéria I.R.
; Rossi, Rogério V.
; Gerstmeier, Roland
; Ochoa, Ronald
; Hutchings, Rosa S.G.
; Ale-Rocha, Rosaly
; Rocha, Rosana M. da
; Tidon, Rosana
; Brito, Rosangela
; Pellens, Roseli
; Santos, Sabrina R. dos
; Santos, Sandra D. dos
; Paiva, Sandra V.
; Santos, Sandro
; Oliveira, Sarah S. de
; Costa, Sávio C.
; Gardner, Scott L.
; Leal, Sebastián A. Muñoz
; Aloquio, Sergio
; Bonecker, Sergio L.C.
; Bueno, Sergio L. de S.
; Almeida, Sérgio M. de
; Stampar, Sérgio N.
; Andena, Sérgio R.
; Posso, Sergio R.
; Lima, Sheila P.
; Gadelha, Sian de S.
; Thiengo, Silvana C.
; Cohen, Simone C.
; Brandão, Simone N.
; Rosa, Simone P.
; Ribeiro, Síria L.B.
; Letana, Sócrates D.
; Santos, Sonia B. dos
; Andrade, Sonia C.S.
; Dávila, Stephane
; Vaz, Stéphanie
; Peck, Stewart B.
; Christo, Susete W.
; Cunha, Suzan B.Z.
; Gomes, Suzete R.
; Duarte, Tácio
; Madeira-Ott, Taís
; Marques, Taísa
; Roell, Talita
; Lima, Tarcilla C. de
; Sepulveda, Tatiana A.
; Maria, Tatiana F.
; Ruschel, Tatiana P.
; Rodrigues, Thaiana
; Marinho, Thais A.
; Almeida, Thaís M. de
; Miranda, Thaís P.
; Freitas, Thales R.O.
; Pereira, Thalles P.L.
; Zacca, Thamara
; Pacheco, Thaynara L.
; Martins, Thiago F.
; Alvarenga, Thiago M.
; Carvalho, Thiago R. de
; Polizei, Thiago T.S.
; McElrath, Thomas C.
; Henry, Thomas
; Pikart, Tiago G.
; Porto, Tiago J.
; Krolow, Tiago K.
; Carvalho, Tiago P.
; Lotufo, Tito M. da C.
; Caramaschi, Ulisses
; Pinheiro, Ulisses dos S.
; Pardiñas, Ulyses F.J.
; Maia, Valéria C.
; Tavares, Valeria
; Costa, Valmir A.
; Amaral, Vanessa S. do
; Silva, Vera C.
; Wolff, Vera R. dos S.
; Slobodian, Verônica
; Silva, Vinícius B. da
; Espíndola, Vinicius C.
; Costa-Silva, Vinicius da
; Bertaco, Vinicius de A.
; Padula, Vinícius
; Ferreira, Vinicius S.
; Silva, Vitor C.P. da
; Piacentini, Vítor de Q.
; Sandoval-Gómez, Vivian E.
; Trevine, Vivian
; Sousa, Viviane R.
; Sant’Anna, Vivianne B. de
; Mathis, Wayne N.
; Souza, Wesley de O.
; Colombo, Wesley D.
; Tomaszewska, Wioletta
; Wosiacki, Wolmar B.
; Ovando, Ximena M.C.
; Leite, Yuri L.R.
.
ABSTRACT The limited temporal completeness and taxonomic accuracy of species lists, made available in a traditional manner in scientific publications, has always represented a problem. These lists are invariably limited to a few taxonomic groups and do not represent up-to-date knowledge of all species and classifications. In this context, the Brazilian megadiverse fauna is no exception, and the Catálogo Taxonômico da Fauna do Brasil (CTFB) (http://fauna.jbrj.gov.br/), made public in 2015, represents a database on biodiversity anchored on a list of valid and expertly recognized scientific names of animals in Brazil. The CTFB is updated in near real time by a team of more than 800 specialists. By January 1, 2024, the CTFB compiled 133,691 nominal species, with 125,138 that were considered valid. Most of the valid species were arthropods (82.3%, with more than 102,000 species) and chordates (7.69%, with over 11,000 species). These taxa were followed by a cluster composed of Mollusca (3,567 species), Platyhelminthes (2,292 species), Annelida (1,833 species), and Nematoda (1,447 species). All remaining groups had less than 1,000 species reported in Brazil, with Cnidaria (831 species), Porifera (628 species), Rotifera (606 species), and Bryozoa (520 species) representing those with more than 500 species. Analysis of the CTFB database can facilitate and direct efforts towards the discovery of new species in Brazil, but it is also fundamental in providing the best available list of valid nominal species to users, including those in science, health, conservation efforts, and any initiative involving animals. The importance of the CTFB is evidenced by the elevated number of citations in the scientific literature in diverse areas of biology, law, anthropology, education, forensic science, and veterinary science, among others. publications problem uptodate up date classifications context exception (CTFB http//fauna.jbrj.gov.br/, httpfaunajbrjgovbr http //fauna.jbrj.gov.br/ , jbrj gov br (http://fauna.jbrj.gov.br/) 2015 Brazil 80 specialists 1 2024 133691 133 691 133,69 125138 125 138 125,13 82.3%, 823 82 3 (82.3% 102000 102 000 102,00 7.69%, 769 7 69 (7.69% 11000 11 11,00 . 3,567 3567 567 (3,56 2,292 2292 2 292 (2,29 1,833 1833 833 (1,83 1,447 1447 447 (1,44 1000 1,00 831 (83 628 (62 606 (60 520 (52 50 users science health biology law anthropology education others http//fauna.jbrj.gov.br/ faunajbrjgovbr //fauna.jbrj.gov.br (http://fauna.jbrj.gov.br/ 201 8 202 13369 13 133,6 12513 12 125,1 82.3% (82.3 10200 10 00 102,0 7.69% 76 6 (7.69 1100 11,0 3,56 356 56 (3,5 2,29 229 29 (2,2 1,83 183 83 (1,8 1,44 144 44 (1,4 100 1,0 (8 62 (6 60 52 (5 5 http//fauna.jbrj.gov.br (http://fauna.jbrj.gov.br 20 1336 133, 1251 125, 82.3 (82. 1020 0 102, 7.69 (7.6 110 11, 3,5 35 (3, 2,2 22 (2, 1,8 18 (1, 1,4 14 4 ( 82. (82 7.6 (7. 3, (3 2, (2 (1 7. (7
7.
Economic indicators for cowpea cultivation under different irrigation depths
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Carvalho, Erika de O. T. de
; Costa, Deborah L. P.
; Luz, Denilson B. da
; Rua, Matheus L.
; Velame, Maria de L. A.
; Monteiro, Adrielle C.
; Vieira, Igor C. de O.
; Pinto, João V. de N.
; Fernandes, Gabriel S. T.
; Nunes, Hildo G. G. C.
; Souza, Paulo J. de O. P. de
; Santos, Marcos A. S. dos
.
Revista Brasileira de Engenharia Agrícola e Ambiental
- Journal Metrics
RESUMO O feijão-caupi apresenta grande importância socioeconômica nas regiões Norte e Nordeste do Brasil, sendo que a busca por alternativas que ofereçam aumento de produtividade com retorno financeiro tem se tornado um importante desafio. Assim, o objetivo deste trabalho foi estimar e avaliar os indicadores econômicos da produção de feijão-caupi nos anos de 2015 e 2016 quando da aplicação de irrigação por gotejamento em diferentes lâminas. Os tratamentos de irrigação foram baseados na evapotranspiração da cultura (ETc). O experimento foi instalado em blocos ao acaso com quatro tratamentos (0, 25, 50 e 100% da ETc) e seis repetições. Para análise econômica, os resultados foram extrapolados para uma área de um hectare. A produtividade em 2015 foi inferior à de 2016; porém, em todos os tratamentos, a produtividade foi sempre maior quando as plantas foram cultivadas sob irrigação adequada. Em ambos os anos, os custos operacionais totais foram maiores sob irrigação do que sob condições de sequeiro. O tratamento 100% ETc resultou em maior número de sacas de feijão-caupi (60 kg) produzidas e receita bruta superior ao tratamento de sequeiro em 17 sacas ha-1, equivalente a 1.020 kg ha-1 (receita bruta US$ 711,48) e 16 sacas ha-1, equivalente a 960 kg ha-1 (receita bruta US$ 867,12), em 2015 e 2016, respectivamente. A deficiência hídrica limitou a produtividade do feijão-caupi nos dois anos, mas inviabilizou economicamente o cultivo apenas em 2015. feijãocaupi feijão caupi Brasil desafio Assim 201 lâminas ETc. . (ETc) 0, 0 (0 25 5 100 repetições econômica hectare porém adequada 60 (6 1 ha1, ha1 ha 1, 1020 020 1.02 ha- US 711,48 71148 711 48 96 867,12, 86712 867,12 , 867 12 867,12) respectivamente 20 (ETc ( 2 10 6 102 02 1.0 711,4 7114 71 4 9 8671 867,1 86 1. 711, 7 867, 8
ABSTRACT Cowpeas are of high socioeconomic importance in the North and Northeast regions of Brazil, whereby the search for alternatives that offer increased productivity with financial returns has become an important challenge. Thus, the objective of this study was to estimate and evaluate the economic indicators of cowpea production in 2015 and 2016 when applying drip irrigation to different depths. Irrigation treatments were based on crop evapotranspiration (ETc). The experiment was laid in a randomized complete blocks design with four treatments (0, 25, 50, and 100% ETc) and six replications. For economic analysis, the results were extrapolated to an area of one hectare. Productivity in 2015 was lower than that in 2016; however, in all treatments, it was always higher when plants were grown under adequate water supply. In both years, the total operating costs were higher under irrigation than under rainfed conditions. The 100% ETc treatment resulted in a greater number of cowpea bags (60 kg) produced and a higher gross revenue than the rainfed treatment by 17 bags ha-1, equivalent to 1,020 kg ha-1 (gross revenue US$ 711.48) and 16 bags ha-1, equivalent to 960 kg ha-1 (gross revenue US$ 867.12), in 2015 and 2016, respectively. Water deficit limited cowpea yield in both years but made cultivation economically unfeasible only in 2015. Brazil challenge Thus 201 depths ETc. . (ETc) 0, 0 (0 25 50 100 replications analysis hectare however supply conditions 60 (6 1 ha1, ha1 ha 1, 1020 020 1,02 ha- US 711.48 71148 711 48 96 867.12, 86712 867.12 , 867 12 867.12) respectively 20 (ETc ( 2 5 10 6 102 02 1,0 711.4 7114 71 4 9 8671 867.1 86 711. 7 867. 8
8.
Toxicity of Agave sisalana extracts on Cordyceps and their effect and the association with fungi on Nasutitermes corniger (Isoptera: Termitidae)
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Da Silva Lopes, Rosineide
; Barroso Martins, Mônica Cristina
; De Lima, Geiziquele
; Gonçalves De Oliveira, Luciana
; Félix Da Costa, Antonio
; Felipe Dos Santos, Venézio
; Dos Santos Correia, Maria Tereza
; Da Silva, Nicácio Henrique
; Correia De Albuquerque, Auristela
; De Luna Alves Lima, Elza Áurea
; De Menezes Lima, Vera Lúcia
.
Resumen Nasutitermes corniger es una termita arbórea que causa daños económicos en las zonas urbanas, y su control es mediante insecticidas químicos. Una alternativa para el control de insectos es el uso de hongos entomopatógenos y extractos de plantas, o el efecto sinérgico de la asociación de estos. Se examinó la toxicidad de los extractos acuosos e hidroetanólicos de Agave sisalana sobre los aspectos biológicos de los hongos Cordyceps farinosa, C. fumosorosea y C. javanica y la acción de los extractos y su efecto sinérgico sobre N. corniger. Se evaluó el efecto de los extractos sobre hongos por germinación, esporulación y crecimiento micelial de los hongos en medio Sabouraud mediante el estudio de aspectos biológicos bajo condiciones de laboratorio y se examinó el control de N. corniger, por ingestión de extractos y de la asociación extracto y hongo sobre papel de filtro, siendo el porcentaje de mortalidad de soldados y obreras de la termita diaria. En general, los extractos resultaron inofensivos para los hongos a concentraciones de 10, 25, 50, 100 y 200 mg.mL-1. Los extractos demostraron acciones termiticidas a las concentraciones ensayadas, provocando la mortalidad de alrededor del 100% de los trabajadores después del tercer y sexto día (LC50: 0.778 a 0.803 mg.mL-1) y del 100% de los soldados entre el cuarto y séptimo días (LC50: 0.146 a 0.956 mg.mL-1). La asociación de los extractos con C. farinosa ESALQ1355 fue más eficiente para inducir la mortalidad en N. corniger. Estos resultados demostraron la eficiencia in vitro de los extractos de sisal en el control de las termitas de N. corniger, solas o en combinación con hongos, lo que sugiere su potencial utilidad conjunta en el control biológico de plagas de insectos.
Abstract Nasutitermes corniger is an arboreal termite that causes economic damage in urban areas, and its control is for chemical insecticides. An alternative for insect control is the use of entomopathogenic fungi and plant extracts, or the synergistic effect of the association of these. The toxicity of aqueous and hydroethanolic extracts of Agave sisalana on the biological aspects of the fungal Cordyceps farinosa, C. fumosorosea and C. javanica and the action of the extracts and their synergistic effect on N. corniger was examined. The effect of the extracts on fungi was evaluated by germination, sporulation and mycelial growth of fungi in Sabouraud medium through the study of biological aspects under laboratory conditions; and control of N. corniger were examined, by ingestion of extracts and of the association extract plus fungus on filter paper, being the percentage of mortality of soldiers and workers of the termite daily. In general, the extracts were inoffensive to the fungi at concentrations 10, 25, 50, 100 and 200 mg.mL-1. The extracts demonstrated termiticidal actions at concentrations tested, causing the mortalities about 100% of the workers after the third and sixth days (LC50: 0.778 to 0.803 mg.mL-1) and 100% of the soldiers between the fourth and seventh days (LC50:0.146 to 0.956 mg.mL-1). The association of the extracts with C. farinosa ESALQ1355 was more efficient in inducing the mortality in N. corniger. These results demonstrated the efficiency in vitro of the sisal extracts in controlling N. corniger termites, alone or in combination with fungi, suggesting their potential joint usefulness in the biological control of insect pests.
9.
[SciELO Preprints] - Guideline of the Brazilian Society of Cardiology on Diagnosis and Treatment of Patients with Chagas Disease Cardiomyopathy
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Marin-Neto, José Antonio
Rassi Jr., Anis
Moraes Oliveira, Gláucia M.
Lemos Correia, Luís Claudio
Novaes Ramos Jr., Alberto
Hasslocher-Moreno, Alejandro Marcel
Luquetti Ostermayer, Alejandro
Sousa, Andréa Silvestre de
Amato Vincenzo de Paola, Angelo
Sobral de Sousa, Antonio Carlos
Pinho Ribeiro, Antonio Luiz
Correia Filho, Dalmo
Moraes de Souza, Dilma do Socorro
Cunha-Neto, Edecio
J. A. Ramires, Felix
Bacal, Fernando
Pereira Nunes, Maria do Carmo
Martinelli Filho, Martino
Ibrahim Scanavacca, Maurício
Magalhães Saraiva, Roberto
Alves de Oliveira Júnior, Wilson
M. Lorga-Filho, Adalberto
de Jesus Benevides de Almeida Guimarães, Adriana
Lopes Latado Braga, Adriana
Sarmento de Oliveira, Adriana
V. L. Sarabanda, Alvaro
Yecê das Neves Pinto, Ana
Assis Lopes do Carmo, André
Schmidt, André
Costa, Andréa Rodrigues da
Ianni, Barbara Maria
Markman Filho, Brivaldo
Eduardo Rochitte, Carlos
Thé Macedo, Carolina
Mady, Charles
Chevillard, Christophe
Bittencourt das Virgens, Cláudio Marcelo
Nery de Castro, Cleudson
De Paoli de Carvalho Britto, Constança Felícia
Pisani, Cristiano
do Carmo Rassi, Daniela
C. Sobral Filho, Dario
Rodrigues Almeida, Dirceu
A. Bocchi, Edimar
T. Mesquita, Evandro
de Souza Nogueira Sardinha Mendes, Fernanda
Pereira, Francisca Tatiana
Sperandio da Silva, Gilberto Marcelo
de Lima Peixoto, Giselle
Glotz de Lima, Gustavo
H. Veloso, Henrique
Turin Moreira, Henrique
Bellotti Lopes, Hugo
Masciarelli Francisco Pinto, Ibraim
Pinto Dias, João Carlos
Bemfica, João Marcos
Silva-Nunes, João Paulo
Soares Barreto-Filho, José Augusto
Kerr Saraiva, José Francisco
Lannes-Vieira, Joseli
Menezes Oliveira, Joselina Luzia
V. Armaganijan, Luciana
Martins, Luiz Cláudio
C. Sangenis, Luiz Henrique
Barbosa, Marco Paulo
Almeida-Santos, Marcos Antônio
Simões, Marcos Vinicius
Shikanai-Yasuda, Maria Aparecida
Vieira Moreira, Maria da Consolação
Higuchi, Maria de Lourdes
Costa Monteiro, Maria Rita de Cássia
Felix Mediano, Mauro Felippe
Maia Lima, Mayara
T. Oliveira, Maykon
Moreira Dias Romano , Minna
Nitz, Nadjar
de Tarso Jorge Medeiros, Paulo
Vieira Alves, Renato
Alkmim Teixeira, Ricardo
Coury Pedrosa, Roberto
Aras, Roque
Morais Torres, Rosália
dos Santos Povoa, Rui Manoel
Rassi, Sérgio Gabriel
Salles Xavier, Sérgio
Marinho Martins Alves , Silvia
B. N. Tavares, Suelene
Lima Palmeira, Swamy
da Silva Junior, Telêmaco Luiz
da Rocha Rodrigues, Thiago
Madrini Junior, Vagner
Maia da Costa , Veruska
Dutra, Walderez
This guideline aimed to update the concepts and formulate the standards of conduct and scientific evidence that support them, regarding the diagnosis and treatment of the Cardiomyopathy of Chagas disease, with special emphasis on the rationality base that supported it.nbsp;
Chagas disease in the 21st century maintains an epidemiological pattern of endemicity in 21 Latin American countries. Researchers and managers from endemic and non-endemic countries point to the need to adopt comprehensive public health policies to effectively control the interhuman transmission of T. cruzi infection, and to obtain an optimized level of care for already infected individuals, focusing on diagnostic and therapeutic opportunistic opportunities.
nbsp;
Pathogenic and pathophysiological mechanisms of the Cardiomyopathy of Chagas disease were revisited after in-depth updating and the notion that necrosis and fibrosis are stimulated by tissue parasitic persistence and adverse immune reaction, as fundamental mechanisms, assisted by autonomic and microvascular disorders, was well established. Some of them have recently formed potential targets of therapies.nbsp;
The natural history of the acute and chronic phases was reviewed, with enhancement for oral transmission, indeterminate form and chronic syndromes. Recent meta-analyses of observational studies have estimated the risk of evolution from acute and indeterminate forms and mortality after chronic cardiomyopathy. Therapeutic approaches applicable to individuals with Indeterminate form of Chagas disease were specifically addressed. All methods to detect structural and/or functional alterations with various cardiac imaging techniques were also reviewed, with recommendations for use in various clinical scenarios. Mortality risk stratification based on the Rassi score, with recent studies of its application, was complemented by methods that detect myocardial fibrosis.nbsp;
The current methodology for etiological diagnosis and the consequent implications of trypanonomic treatment deserved a comprehensive and in-depth approach. Also the treatment of patients at risk or with heart failure, arrhythmias and thromboembolic events, based on pharmacological and complementary resources, received special attention. Additional chapters supported the conducts applicable to several special contexts, including t. cruzi/HIV co-infection, risk during surgeries, in pregnant women, in the reactivation of infection after heart transplantation, and others.nbsp; nbsp;nbsp;
Finally, two chapters of great social significance, addressing the structuring of specialized services to care for individuals with the Cardiomyopathy of Chagas disease, and reviewing the concepts of severe heart disease and its medical-labor implications completed this guideline.
Esta diretriz teve como objetivo principal atualizar os conceitos e formular as normas de conduta e evidências científicas que as suportam, quanto ao diagnóstico e tratamento da CDC, com especial ênfase na base de racionalidade que a embasou.
A DC no século XXI mantém padrão epidemiológico de endemicidade em 21 países da América Latina. Investigadores e gestores de países endêmicos e não endêmicos indigitam a necessidade de se adotarem políticas abrangentes, de saúde pública, para controle eficaz da transmissão inter-humanos da infecção pelo T. cruzi, e obter-se nível otimizado de atendimento aos indivíduos já infectados, com foco em oportunização diagnóstica e terapêutica.
Mecanismos patogênicos e fisiopatológicos da CDC foram revisitados após atualização aprofundada e ficou bem consolidada a noção de que necrose e fibrose sejam estimuladas pela persistência parasitária tissular e reação imune adversa, como mecanismos fundamentais, coadjuvados por distúrbios autonômicos e microvasculares. Alguns deles recentemente constituíram alvos potenciais de terapêuticas.
A história natural das fases aguda e crônica foi revista, com realce para a transmissão oral, a forma indeterminada e as síndromes crônicas. Metanálises recentes de estudos observacionais estimaram o risco de evolução a partir das formas aguda e indeterminada e de mortalidade após instalação da cardiomiopatia crônica. Condutas terapêuticas aplicáveis aos indivíduos com a FIDC foram abordadas especificamente. Todos os métodos para detectar alterações estruturais e/ou funcionais com variadas técnicas de imageamento cardíaco também foram revisados, com recomendações de uso nos vários cenários clínicos. Estratificação de risco de mortalidade fundamentada no escore de Rassi, com estudos recentes de sua aplicação, foi complementada por métodos que detectam fibrose miocárdica.
A metodologia atual para diagnóstico etiológico e as consequentes implicações do tratamento tripanossomicida mereceram enfoque abrangente e aprofundado. Também o tratamento de pacientes em risco ou com insuficiência cardíaca, arritmias e eventos tromboembólicos, baseado em recursos farmacológicos e complementares, recebeu especial atenção. Capítulos suplementares subsidiaram as condutas aplicáveis a diversos contextos especiais, entre eles o da co-infecção por T. cruzi/HIV, risco durante cirurgias, em grávidas, na reativação da infecção após transplante cardíacos, e outros.nbsp;nbsp;nbsp;
Por fim, dois capítulos de grande significado social, abordando a estruturação de serviços especializados para atendimento aos indivíduos com a CDC, e revisando os conceitos de cardiopatia grave e suas implicações médico-trabalhistas completaram esta diretriz.nbsp;
10.
Molecular detection and phylogenetic analysis of Trypanosoma spp. in Neotropical primates from Rio de Janeiro State, Brazil
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Guimarães, Andresa
; Santos, Huarrisson A.
; Balthazar, Daniel A.
; Kierulff, Maria Cecília M.
; Baptista, Michelle N.M.
; Oliveira, Ágatha F.X.
; Stocco, Naiara V.
; Mureb, Elisabeth N.
; Costa, Alexandre C.
; Raimundo, Juliana M.
; Baldani, Cristiane D.
.
RESUMO: A infecção por Trypanosoma spp. é um problema em muitos países tropicais, infectando várias espécies animais, incluindo humanos. O objetivo do presente estudo foi identificar as espécies de Trypanosoma em primatas neotropicais no estado Rio de Janeiro e comparar os resultados com outros relatos, tanto filogeneticamente quando geograficamente. A detecção molecular foi baseada no gene SSU 18. As sequências obtidas na PCR foram sequenciadas e comparadas com outras previamente depositadas no GenBank. Essas sequências foram utilizadas para análises filogenéticas e confeccionar um mapa de distribuição de espécies de primatas infectadas por espécies de Trypanosoma no Brasil. Entre 34 macacos, cinco macacos-prego (Sapajus spp.) e um sagui (Callithrix spp.) apresentaram sequências de Trypanosoma spp. no mesmo clado de Trypanosoma minasense e três sequências de macacos-prego estavam no mesmo clado de Trypanosoma cruzi. A Mata Atlântica e a Amazônia brasileira são as regiões com maior frequência de estudos sobre Trypanosoma spp. e variedade de primatas neotropicais hospedeiros. São áreas que merecem atenção no que se refere à conservação da biodiversidade, mas também evidencia a carência de estudos com PNH em outras regiões do país e de estudos multidisciplinares para melhor compreender as relações do patógeno hospedeiro.
ABSTRACT: Trypanosoma spp. infection is a problem in many tropical countries, infecting several animal species, including humans. The aim of the present study was to identify the Trypanosoma species in Neotropical primates from Rio de Janeiro state and compare the results with other reports both phylogenetically and geographically. Molecular detection was based on the 18 SSU gene. The sequences obtained in the PCR were sequenced and compared with others previously deposited in GenBank. These sequences were used to perform phylogenetic analysis and make a distribution map of primate species infected by Trypanosoma species in Brazil. Among 34 monkeys, five capuchin monkeys (Sapajus spp.) and one marmoset (Callithrix spp.) showed Trypanosoma spp. sequences in the same clade of Trypanosoma minasense and three capuchin monkeys’ sequences were in the same clade of Trypanosoma cruzi. The Atlantic Forest and the Brazilian Amazon are the regions with the highest frequency of studies about Trypanosoma spp. and variety of Neotropical primate hosts. These are areas that deserve attention regarding the conservation of biodiversity, but it also makes evident the lack of studies with Neotropical primates in other regions of the country, as well as multidisciplinary studies to better understand the host pathogen relationships.
11.
Ultrasensitive molecular tests for Plasmodium detection: applicability in control and elimination programs and reference laboratories
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Aschar, Mariana
; Sanchez, Maria Carmen A.
; Costa-Nascimento, Maria de Jesus
; Farinas, Maria de Lourdes R. N.
; Hristov, Angélica D.
; Lima, Giselle F. M. C.
; Inoue, Juliana
; Levi, José E.
; Di Santi, Silvia M.
.
RESUMO Objectivo. Avaliar ferramentas moleculares para detectar parasitemia de baixo nível e as cinco espécies de Plasmodium que infectam humanos, para utilização em programas de controlo e eliminação e em laboratórios de referência. Métodos. Avaliámos 145 amostras de sangue de doentes que testaram positivo por reacção em cadeia da polimerase aninhada (nPCR), de indivíduos assintomáticos, e do Programa Global de Paludismo da Organização Mundial de Saúde/Serviço Nacional de Avaliação da Qualidade Externa do Reino Unido. As amostras foram ensaiadas utilizando o RealStar® Malaria PCR Kit 1.0 (alt-Gen; altona Diagnostics) e o RealStar® Malaria Screen & Type PCR Kit (alt-S&T; altona Diagnostics). Os resultados dos testes moleculares foram comparados com os resultados da PCR quantitativa (qPCR), nPCR e exame da gota espessa. Resultados. Os níveis de parasitemia variaram de 1 a 518 000 parasitas/µL, dependendo da espécie. Em comparação com a nPCR, alt-S&T tinha uma sensibilidade de 100%, excepto na identificação de P. falciparum, para a qual a sensibilidade era de 93,94%. Todas as amostras positivas por alt-Gen foram também positivas por nPCR. Ao comparar alt-Gen com qPCR, a sensibilidade foi de 100% para P. vivax, P. malariae e P. falciparum. Para todas as espécies Plasmodium, a correlação entre os valores limiares de ciclo de alt-S&T e alt-Gen comparados com qPCR foi significativa (P < 0,0001, teste de Spearman), com r = 0,8621 para alt-S&T e r = 0,9371 para alt-Gen. Quando todas as espécies de Plasmodium foram consideradas, houve uma correlação negativa entre o nível de parasitemia e os valores limiares do ciclo de PCR em tempo real (P < 0,0001). Neste estudo, apenas 2 de 28 amostras de indivíduos assintomáticos foram positivas por exame da gota espessa; no entanto, todas estas 28 amostras foram positivas por alt-S&T. Conclusões. Os ensaios alt-Gen e alt-S&T são adequados para a detecção de infecções submicroscópicas para fins epidemiológicos distintos, tais como para utilização em inquéritos e laboratórios de referência e o rastreio em bancos de sangue, o que contribuirá para os esforços globais de eliminação da malária.
ABSTRACT Objective. To evaluate molecular tools to detect low-level parasitemia and the five species of Plasmodium that infect humans for use in control and elimination programs, and in reference laboratories. Methods. We evaluated 145 blood samples from patients who tested positive by nested polymerase chain reaction (nPCR), from asymptomatic individuals and from the WHO Global Malaria Programme/United Kingdom National External Quality Assessment Service. Samples were assayed using the genus-specific RealStar® Malaria PCR Kit 1.0 (alt-Gen; altona Diagnostics) and the RealStar® Malaria Screen & Type PCR Kit (alt-S&T; altona Diagnostics). The results from the molecular tests were compared with those from quantitative PCR (qPCR), nPCR and thick blood smear. Results. The levels of parasitemia ranged from 1 to 518 000 parasites/µL, depending on the species. Compared with nPCR, alt-S&T had a sensitivity of 100%, except for identifying P. falciparum, for which the sensitivity was 93.94%. All samples positive by alt-Gen were also positive by nPCR. When comparing alt-Gen to qPCR, the sensitivity was 100% for P. vivax, P. malariae and P. falciparum. For all Plasmodium species, the correlation between cycle threshold values of alt-S&T and alt-Gen compared with qPCR was significant (P < 0.0001, Spearman’s test), with r = 0.8621 for alt-S&T and r = 0.9371 for alt-Gen. When all Plasmodium species were considered, there was a negative correlation between the level of parasitemia and real-time PCR cycle threshold values (P < 0.0001). In this study, only 2 of 28 samples from asymptomatic individuals were positive by thick blood smear; however, all 28 of these samples were positive by alt-S&T. Conclusions. The alt-Gen and alt-S&T assays are suitable for detecting submicroscopic infections for distinct epidemiological purposes, such as for use in surveys and reference laboratories, and screening in blood banks, which will contribute to global efforts to eliminate malaria.
RESUMEN Objetivo. Evaluar herramientas moleculares para detectar bajos niveles de parasitemia y las cinco especies de Plasmodium que infectan a los seres humanos, a fin de emplearlas en los programas de control y eliminación y en los laboratorios de referencia. Métodos. Se evaluaron 145 muestras de sangre de pacientes positivos por reacción en cadena de la polimerasa anidada (nPCR), de individuos asintomáticos y de muestras del Programa Mundial de Malaria de la Organización Mundial de la Salud/Servicio Nacional de Evaluación Externa de Calidad del Reino Unido. Las muestras se analizaron con el kit de PCR RealStar® Malaria 1.0 (alt-Gen; altona Diagnostics), específico para cada género, y con el kit de PCR RealStar® Malaria Screen & Type (alt-S&T; altona Diagnostics). Se compararon los resultados de las pruebas moleculares con los de la PCR cuantitativa (qPCR), la nPCR y el frotis de gota gruesa. Resultados. Los niveles de parasitemia oscilaron entre 1 y 518 000 parásitos/µl, según la especie. En comparación con la nPCR, la prueba alt-S&T tuvo una sensibilidad del 100%, excepto para la identificación de P. falciparum, para el cual la sensibilidad fue del 93,94%. Todas las muestras positivas por alt-Gen lo fueron también por nPCR. Al comparar alt-Gen con la qPCR, la sensibilidad fue del 100% para P. vivax, P. malariae y P. falciparum. Para todas las especies de Plasmodium, la correlación entre los valores del umbral de ciclo de alt-S&T y alt-Gen en comparación con la qPCR fue significativa (P < 0,0001, prueba de Spearman), con r = 0,8621 para alt-S&T y r = 0,9371 para alt-Gen. Cuando se consideraron todas las especies de Plasmodium hubo una correlación negativa entre el nivel de parasitemia y los valores de umbral de ciclo de PCR en tiempo real (P < 0,0001). En este estudio, solo 2 de las 28 muestras de individuos asintomáticos fueron positivas por frotis de gota gruesa; sin embargo, las 28 muestras fueron positivas por alt-S&T. Conclusiones. Los ensayos alt-Gen y alt-S&T son adecuados para detectar infecciones submicroscópicas con distintos fines epidemiológicos, como su uso en investigaciones y laboratorios de referencia y el cribado en bancos de sangre, lo que contribuirá a los esfuerzos mundiales para eliminar la malaria.
12.
Gender-related differences in the modulation of anthropometric, biochemical, and immune markers by physical activity in hypertensive and diabetic individuals
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SANTOS, DÉBORAH C. DOS
; RIBEIRO, ISRAEL S.
; LOPES, DIEGO PATRICK S.
; PEREIRA, ÍTALO S.
; SANTOS, DENISAR P.
; MUNIZ, IGOR P.R.
; LIMA, FERNANDA M. DA SILVA
; COSTA, ANDRESSA ANTÔNIA S.
; SOUZA, DANIELA DA SILVA P.
; CALADO, STÉFANO PEDRO DE MELO
; GONÇALVES, CAROLINE V.
; GALANTINI, MARIA POLIANA L.
; LOPES, DANILO N.
; SILVA, ROBSON A.A. DA
; SANTOS, GILVANÉIA S.
.
Abstract Systemic arterial hypertension (SAH) and type 2 diabetes mellitus (T2DM) compose the two major noncommunicable chronic inflammatory diseases. Physical activity has been shown as a promising complementary approach to control the systemic inflammation. However, it is still unclear whether this modulation is gender-dependent. The objective of this study was evaluate the gender-related influence of physical activity on the inflammatory response and biochemical profile of individuals with SAH and T2DM. An international physical activity questionnaire was applied to 376 individuals diagnosed with SAH and T2DM in order to access their exercises routine and was evaluated the influence of physical activity in biochemical, anthropometrical, and immunological markers involved in these disorders in men and women. Even though active individuals have exhibited lower serum levels of IL-1β, IFN-γ, TNF-α, and IL-17A, the ratios between IL-10 and all inflammatory cytokines were higher in men than in women. Physically active individuals also demonstrated increased HDL/LDL and HDL/VLDL ratios. Moreover, multiple correlations revealed that in active women both IL-10 and TNF-α serum levels positively correlate with fasting glucose levels, and were negatively associated with HDL levels. Our findings suggest that gender-related differences dictate a distinct crosstalk between inflammatory and biochemical markers in physically active individuals.
https://doi.org/10.1590/0001-3765202120201905
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13.
Isolation, Absolute Configuration and Cytotoxic Activities of Alkaloids from Hippeastrum goianum (Ravenna) Meerow (Amaryllidaceae)
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Lianza, Mariacaterina
; Verdan, Maria Helena
; Andrade, Jean Paulo de
; Poli, Ferruccio
; Almeida, Larissa C. de
; Costa-Lotufo, Leticia V.
; Cunha Neto, Álvaro
; Oliveira, Sarah C. C.
; Bastida, Jaume
; Batista, Andrea N. L.
; Batista Jr., João M.
; Borges, Warley S.
.
Journal of the Brazilian Chemical Society
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The phytochemical study of Hippeastrum goianum led to the identification of 13 compounds by means of gas chromatography-mass spectrometry (GC-MS) and nuclear magnetic resonance (NMR). Compounds 7-demethoxy-9-O-methylhostasine (1) and 7-deoxi-trans-dihydronarciclasine (2) had their absolute configurations determined by vibrational circular dichroism (VCD). This is the first time that compound 1 is described in the Amaryllidaceae family. The cytotoxicity of all isolated compounds was tested against colorectal carcinoma (HCT 116), breast carcinoma (MCF-7), and non-tumor human retinal pigment epithelium (RPE) cell lines. The half-maximum inhibitory concentration (IC50) of compound 2 against each cell line was equivalent to the positive control (doxorubicin), indicating a considerable cytotoxic activity.
https://doi.org/10.21577/0103-5053.20200116
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14.
Modelos de agricultura sustentável: biodinâmica e sistema silvipastoril
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Mascarenhas¹, Nágela M. H.
; Furtado¹, Dermeval A.
; Cavalcante¹, Cácio R.
; Costa², Antonio N. L. da
; Souza², Maria A. de
; Gonçalves¹, Mailson G.
; Oliveira¹, Airton G. de
; Brito¹, Alicia N. dos S. L. de
; Silva¹, Luís P. F. R. da
; Nogueira¹, Lídia P. da S.
; Dornelas¹, Karoline C.
; Rodrigues¹, Raimundo C. M.
; Neto¹, Moises S. de Medeiros
; Paiva¹, Francisco J. da S.
; Silva¹, Semirames do N.
.
RESUMO O objetivo desta revisão foi comparar dois modelos de agricultura sustentáveis, demostrando as vantagens e desvantagens de ambos, correlacionando-os entre si. As diversas práticas agrícolas contemporâneas compreendem modelos alternativos de agricultura, como permacultura, agricultura biológica, orgânica, biodinâmica, que são consideradas sustentáveis, e modelos intensivos de produção, que apresentam vários pontos negativos, contrastando com os modelos alternativos. A busca por uma agricultura menos agressiva ao meio ambiente, que permaneça produtiva em longo prazo, tem sido uma preocupação constante de pesquisadores e produtores. Os modelos de agricultura assemelham-se entre si, o que os difere são apenas algumas práticas que não são permitidas em todos, e suas bases de fundamentação. Nesta revisão dois modelos foram mais abordados de forma simplificada, para a percepção dos seus conceitos e de suas bases fundamentais, mostrando o que os diferencia, e que mesmo assim os leva para um mesmo objetivo, criar um ecossistema independente de recursos externos.
ABSTRACT The objective of this review was to compare two models of sustainable agriculture, demonstrating their advantages and disadvantages and correlating them with each other. The diverse contemporary agricultural practices comprise from alternative models of agriculture, such as permaculture, organic farming, organic, biodynamic, that are considered sustainable, and intensive production models, that present several negative points, contrasting with alternative models. The search for a less aggressive agriculture to the environment, what stay productive in the long run, it has been a constant concern of researchers and producers. Agricultural models are similar to each other, what differs are some practices that do not are allowed in all, and its foundation bases. In this review, two models were more approached in a simplified way, to the perception of their concepts and fundamental bases, showing what differentiates them, and that still leads to the same goal, creating an ecosystem independent of external resources.
https://doi.org/10.19084/rca.20853
46 downloads
15.
Modeling and thermodynamic properties of ‘bacaba’ pulp drying
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Morais, Maria F. de
; Santos, José R. O. dos
; Santos, Marisângela P. dos
; Santos, Dyego da C.
; Costa, Tiago N. da
; Lima, Joel B.
.
Revista Brasileira de Engenharia Agrícola e Ambiental
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RESUMO Objetivou-se secar a polpa de bacaba em diferentes condições térmicas, ajustar diferentes modelos matemáticos às curvas de desidratação e calcular os coeficientes de difusão, a energia de ativação e as propriedades termodinâmicas do processo. Frutas da bacabeira foram despolpadas para a obtenção da polpa que foi secada nas temperaturas de 40, 50 e 60 °C e espessura de 1,0 cm. O aumento da temperatura de secagem reduziu os tempos de desidratação, bem como os teores de água de equilíbrio, sendo registradas, no início do processo, taxas de secagem com magnitudes de 0,65; 1,04 e 1,25 kg kg min-1 para as temperaturas de 40, 50 e 60 °C, respectivamente. A equação de Midilli foi selecionada como a mais adequada para predizer o fenômeno de secagem, apresentando os maiores R2, os menores valores de desvio quadrado médio (DQM) e χ2 na maioria das condições térmicas, e distribuição aleatória dos resíduos em todas as condições experimentais. Os coeficientes de difusão efetivos aumentaram com incrementos de temperatura, apresentando-se com magnitudes na ordem de 10-9 m2 s-1, sendo descrita satisfatoriamente pela equação de Arrhenius, que apresentou energia de ativação (Ea) de 37,01 kJ mol-1. A secagem foi caracterizada como endergônica, na qual a entalpia (ΔH) e a entropia (ΔS) reduziram com a ampliação da temperatura, enquanto que a energia livre de Gibbs (ΔG) foi aumentada.
ABSTRACT This study aimed to dry ‘bacaba’ (Oenocarpus bacaba Mart.) pulp under different thermal conditions, fit different mathematical models to the dehydration curves, and calculate the diffusion coefficients, activation energy and thermodynamic properties of the process. ‘Bacaba’ fruits were meshed to obtain the pulp, which was dried at temperatures of 40, 50 and 60 °C and with thickness of 1.0 cm. Increase in drying temperature reduced the dehydration times, as well as the equilibrium moisture contents, and drying rates of 0.65, 1.04 and 1.25 kg kg min-1 were recorded at the beginning of the process for temperatures of 40, 50 and 60 °C, respectively. The Midilli’s equation was selected as the most appropriate to predict the drying phenomenon, showing the highest R2, lowest values of mean square deviation (MSD) and χ2 under most thermal conditions, and random distribution of residuals under all experimental conditions. The effective diffusion coefficients increased with increasing temperature, with magnitudes of the order of 10-9 m2 s-1, being satisfactorily described by the Arrhenius equation, which showed activation energy (Ea) of 37.01 kJ mol-1. The drying process was characterized as endergonic, in which enthalpy (ΔH) and entropy (ΔS) reduced with the increment of temperature, while Gibbs free energy (ΔG) was increased.
https://doi.org/10.1590/1807-1929/agriambi.v23n9p702-708
691 downloads
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