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Acute kidney injury: the experience of a tertiary center of Pediatric Nephrology injury
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Figueiredo, Carolina

; Rocha, Ana Margarida

; Correia-Costa, Liane

; Faria, Maria do Sameiro

; Costa, Teresa

; Mota, Conceição

Brazilian Journal of Nephrology

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sep 2024, Volume 46 N. 3 elocation e20240012

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO Introdução: Insuficiência renal aguda (IRA) é uma deterioração abrupta da função renal. A incidência de IRA pediátrica está aumentando em todo o mundo, em ambientes críticos e não críticos. Nosso objetivo foi caracterizar apresentação, etiologia, evolução e desfechos da IRA em pacientes pediátricos internados em um centro de atendimento terciário. Métodos: Realizamos estudo retrospectivo observacional de centro único de pacientes com idade entre 29 dias a 17 anos e 365 dias internados em nossa Unidade de Nefrologia Pediátrica, de janeiro de 2012 a dezembro de 2021, com diagnóstico de IRA. A gravidade da IRA foi categorizada de acordo com os critérios do Kidney Disease Improving Global Outcomes (KDIGO). Os desfechos considerados foram óbito ou sequelas (proteinúria, hipertensão ou alterações na função renal em avaliações de acompanhamento de 3 a 6 meses). Resultados: Incluímos 46 pacientes com idade mediana de 13,0 (3,5–15,5) anos. Cerca de metade (n = 24; 52,2%) apresentou um fator de risco identificável para o desenvolvimento de IRA. Treze pacientes (28,3%) eram anúricos; todos foram classificados como IRA KDIGO 3 (p < 0,001). Quase um quarto (n = 10; 21,7%) dos pacientes necessitaram de terapia renal substitutiva. Aproximadamente 60% (n = 26) apresentou pelo menos uma sequela, sendo proteinúria a mais comum (n = 15; 38,5%; mediana (P25–75) da relação proteína/creatinina urinária 0,30 (0,27–0,44) mg/mg), seguida de taxa de filtração glomerular (TFG) reduzida (n = 11; 27,5%; mediana (P25–75) da TFG 75 (62–83) mL/min/1,73 m2). Conclusões: A IRA pediátrica está associada à morbidade substancial, com potencial para desenvolvimento de proteinúria e comprometimento da função renal e impacto relevante no prognóstico de longo prazo. Introdução (IRA mundo apresentação etiologia terciário Métodos 2 1 36 Pediátrica 201 2021 KDIGO. . (KDIGO) proteinúria, (proteinúria meses. meses meses) Resultados 4 130 13 0 13, 3,5–15,5 35155 5 15 (3,5–15,5 n 24 52,2% 522 52 28,3% 283 28 (28,3% anúricos p 0,001. 0001 0,001 001 0,001) 10 21,7% 217 21 7 substitutiva 60 26 sequela 38,5% 385 38 P25–75 P2575 P P25 (P25–75 proteínacreatinina proteína creatinina 030 30 0,3 0,27–0,44 027044 27 44 (0,27–0,44 mg/mg, mgmg mg/mg , mg mg/mg) (TFG 11 27,5% 275 62–83 6283 62 83 (62–83 mLmin173 mLmin mL min 73 mL/min/1,7 m2. m2 m m2) Conclusões substancial prazo 20 202 (KDIGO 3,5–15, 3515 (3,5–15, 52,2 28,3 (28,3 000 0,00 00 21,7 38,5 P25–7 P257 P2 (P25–7 03 0, 0,27–0,4 02704 (0,27–0,4 27,5 62–8 628 8 (62–8 mLmin17 mL/min/1, 3,5–15 351 (3,5–15 52, 28, (28, 0,0 21, 38, P25– (P25– 0,27–0, 0270 (0,27–0, 27, 62– (62– mLmin1 mL/min/1 3,5–1 35 (3,5–1 (28 (P25 0,27–0 027 (0,27–0 (62 mL/min/ 3,5– (3,5– (2 (P2 0,27– 02 (0,27– (6 mL/min 3,5 (3,5 ( (P 0,27 (0,27 3, (3, 0,2 (0,2 (3 (0, (0

ABSTRACT Introduction: Acute kidney injury (AKI) is an abrupt deterioration of kidney function. The incidence of pediatric AKI is increasing worldwide, both in critically and non-critically ill settings. We aimed to characterize the presentation, etiology, evolution, and outcome of AKI in pediatric patients admitted to a tertiary care center. Methods: We performed a retrospective observational single-center study of patients aged 29 days to 17 years and 365 days admitted to our Pediatric Nephrology Unit from January 2012 to December 2021, with the diagnosis of AKI. AKI severity was categorized according to Kidney Disease Improving Global Outcomes (KDIGO) criteria. The outcomes considered were death or sequelae (proteinuria, hypertension, or changes in renal function at 3 to 6 months follow-up assessments). Results: Forty-six patients with a median age of 13.0 (3.5–15.5) years were included. About half of the patients (n = 24, 52.2%) had an identifiable risk factor for the development of AKI. Thirteen patients (28.3%) were anuric, and all of those were categorized as AKI KDIGO stage 3 (p < 0.001). Almost one quarter (n = 10, 21.7%) of patients required renal replacement therapy. Approximately 60% of patients (n = 26) had at least one sequelae, with proteinuria being the most common (n = 15, 38.5%; median (P25–75) urinary protein-to-creatinine ratio 0.30 (0.27–0.44) mg/mg), followed by reduced glomerular filtration rate (GFR) (n = 11, 27.5%; median (P25–75) GFR 75 (62–83) mL/min/1.73 m2). Conclusions: Pediatric AKI is associated with substantial morbidity, with potential for proteinuria development and renal function impairment and a relevant impact on long-term prognosis. Introduction (AKI worldwide noncritically non settings presentation etiology evolution center Methods singlecenter single 2 1 36 201 2021 (KDIGO criteria proteinuria, (proteinuria hypertension followup follow up assessments. assessments . assessments) Results Fortysix Forty six 130 13 0 13. 3.5–15.5 35155 5 15 (3.5–15.5 included n 24 52.2% 522 52 28.3% 283 28 (28.3% anuric p 0.001. 0001 0.001 001 0.001) 10 21.7% 217 21 7 therapy 60 26 38.5% 385 38 P25–75 P2575 P P25 (P25–75 proteintocreatinine protein creatinine 030 30 0.3 0.27–0.44 027044 27 44 (0.27–0.44 mg/mg, mgmg mg/mg , mg mg/mg) (GFR 11 27.5% 275 62–83 6283 62 83 (62–83 mLmin173 mLmin mL min 73 mL/min/1.7 m2. m2 m m2) Conclusions morbidity longterm long term prognosis 20 202 3.5–15. 3515 (3.5–15. 52.2 28.3 (28.3 000 0.00 00 21.7 38.5 P25–7 P257 P2 (P25–7 03 0. 0.27–0.4 02704 4 (0.27–0.4 27.5 62–8 628 8 (62–8 mLmin17 mL/min/1. 3.5–15 351 (3.5–15 52. 28. (28. 0.0 21. 38. P25– (P25– 0.27–0. 0270 (0.27–0. 27. 62– (62– mLmin1 mL/min/1 3.5–1 35 (3.5–1 (28 (P25 0.27–0 027 (0.27–0 (62 mL/min/ 3.5– (3.5– (2 (P2 0.27– 02 (0.27– (6 mL/min 3.5 (3.5 ( (P 0.27 (0.27 3. (3. 0.2 (0.2 (3 (0. (0

https://doi.org/10.1590/2175-8239-jbn-2024-0012en

Salivary cytokine profile in patients with oral lichen planus
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Humberto, Janaina Silva Martins

; Saia, Rafael Simone

; Costa, Luis Henrique Angenendt

; Rocha, Maria José Alves

; Motta, Ana Carolina Fragoso

Odovtos International Journal of Dental Sciences

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aug 2024, Volume 26 N. 2 Pages 128 - 140

Abstract: Es En | Text: Es En | PDF: Es | PDF: En

Resumen Este estudio tuvo como objetivo determinar las concentraciones salivales de interleucina (IL)-1β, IL-2, IL-10, IL-23, factor de crecimiento transformante (TGF)-β y factor de necrosis tumoral (TNF)-α, nitrato (subproducto de la oxidación del óxido nítrico) y cortisol en pacientes con liquen plano oral (OLP). En este estudio transversal se incluyeron veinte pacientes diagnosticados con OLP y 20 voluntarios sanos (HV) del mismo sexo. Saliva entera no estimulada Se recolectó por la mañana, se determinaron las concentraciones de citocinas y cortisol en saliva mediante ensayo inmunoabsorbente ligado a enzimas (ELISA), se determinó nitrato mediante un analizador de óxido nítrico, se encontraron concentraciones salivales mayores de IL-2 (p<0,003), IL- 23 (p<0,04) y TGF-β (p=0,05) en pacientes con OLP en comparación con HV. No se encontraron diferencias significativas en los niveles salivales de TNF-α, IL-1β e IL-10. Las concentraciones de nitrato fueron marcadamente aumentó en pacientes con OLP (1227,0 ± 738,8 µM/mg de proteína total), en comparación con HV (261,6 ± 166,8 µM/mg; p<0,0001). Los niveles de cortisol salival también fueron más altos en los pacientes con OLP que en los controles (2,79 ± 1,39 vs. 1,94 ±1,21 ng/mg; p<0,048). Los niveles de óxido nítrico en saliva aumentaron notablemente en pacientes con OLP, lo que sugiere una relación de esta molécula con la muerte celular y el daño tisular observado en las lesiones de OLP.

Abstract This study aimed to determine salivary concentrations of interleukin (IL)-1β, IL-2, IL-10, IL- 23, transforming growth factor (TGF)-β, tumor necrosis factor (TNF)-α, nitrate (a by-product of nitric oxide oxidation), and cortisol in patients with oral lichen planus (OLP). Twenty patients diagnosed with OLP and 20 sex-matched healthy volunteers (HV) were included in this cross-sectional study. Unstimulated whole saliva was collected in the morning. Salivary cytokine and cortisol concentrations were determined by enzyme-linked immunosorbent assays (ELISA). Nitrate was measured in a nitric oxide analyzer. We found higher salivary concentrations of IL-2 (p<0.003), IL-23 ( p<0.04), and TGF-β (p=0.05) in patients with OLP compared to HV. No significant differences were found in salivary levels of TNF-α, IL-1β, or IL-10. Nitrate concentrations were markedly increased in OLP patients (1,227.0 ± 738.8 µM/mg total protein) when compared to HV (261.6 ± 166.8 µM/mg; p<0.0001). Salivary cortisol levels were also higher in OLP patients (2.79 ± 1.39 vs. 1.94 ±1.21 ng/mg; p<0.048). The markedly increased salivary levels of nitric oxide in patients with OLP suggest a relationship of this molecule with the cell death and tissue damage observed in these lesions.

https://doi.org/10.15517/ijds.2023.57139

Gasto catastrófico em crianças com deficiência visual: estudo transversal com cuidadores no Rio de Janeiro, Brasil
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Barros, Letícia Baptista de Paula

; Zin, Andrea

; Moreira, Martha Cristina Nunes

; Santos, Marisa da Silva

; Costa, Ana Carolina Carioca da

; Campos, Daniel de Souza

; Neves, Luiza M.

; Haefeli, Lorena

; Entringer, Aline Piovezan

; Moreira, Maria Elisabeth Lopes

; Rebelo, Fernanda

; Vasconcelos, Zilton Farias Meira de

; Pinto, Márcia

Cadernos de Saúde Pública

Journal Metrics
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2024, Volume 40 N. 9 elocation e00167723

Abstract: Pt Es En | Text: Pt Es En | PDF: Pt | PDF: Es | PDF: En

Resumo: O cuidado de um filho com deficiência visual pode vir a afetar a renda do cuidador e, por sua vez, a renda da família. Sob essa realidade, há de se considerar o gasto catastrófico consequente do aumento de despesas e da redução de renda, seja pelo desemprego, pela redução do número de horas trabalhadas ou pela dificuldade de (re)inserção no mercado de trabalho. Perante esse cenário, o objetivo principal deste estudo foi estimar o gasto catastrófico atribuído ao cuidador de crianças cegas ou com baixa visão em centros de referência em educação para cegos, oftalmologia e saúde infantil localizados no Município do Rio de Janeiro, Brasil, identificando quais fatores estão associados a uma maior ou menor prevalência desse gasto. Observou-se que 53,3% dos cuidados de crianças com cegueira comprometem 40% ou mais da renda. Entre os cuidadores de crianças com baixa visão, o gasto catastrófico é mais ameno, comprometendo no mínimo 40% da renda para 36,8% dos cuidadores. Os fatores associados à maior prevalência de gasto catastrófico foram idade do cuidador, número de moradores na residência, maior escolaridade, menor renda domiciliar, reformas na residência, plano de saúde, aquisição de empréstimos, venda de bens, quantidade de unidades de saúde que a criança recebe tratamento e parentesco do cuidador principal. A carga que recai sobre os cuidadores de crianças com deficiência visual indica uma situação de vulnerabilidade que mostra a necessidade de acesso aos mecanismos de proteção financeira e social, por meio de políticas que sejam capazes de atender esse grupo.

Resumen: El cuidado de un niño con discapacidad visual puede impactar los ingresos del cuidador y, a su vez, de la familia. En este escenario, es necesario considerar el gasto catastrófico resultante del aumento de los gastos o la reducción de los ingresos, ya sea por desempleo, reducción del número de horas trabajadas o por la dificultad de inserción o reinserción en el mercado laboral. Ante esto, el objetivo principal de este estudio fue estimar el gasto catastrófico atribuido al cuidador de niños ciegos o con baja visión en centros de referencia en educación para ciegos, oftalmología y salud infantil, ubicados en el municipio de Río de Janeiro, Brasil, con el fin de identificar qué factores se asocian con una mayor o menor prevalencia de este gasto. Se observó que el 53,3% de los cuidados de niños con discapacidad visual comprometen más del 40% de los ingresos totales. Mientras tanto, el cuidado de niños con baja visión tiene un menor gasto catastrófico, comprometiendo menos del 40% de los ingresos según el 36,8% de los cuidadores. La mayor prevalencia de gasto catastrófico estuvo asociada a los siguientes factores: edad del cuidador, número de residentes en el hogar, mayor nivel de estudios, bajos ingresos familiares, remodelaciones en el hogar, seguro de salud, adquisición de préstamos, venta de bienes, cantidad de centros de salud en las que el niño acude al tratamiento y parentesco del cuidador principal. Los cuidadores de niños con discapacidad visual enfrentan una situación de vulnerabilidad, lo que apunta a una necesidad de acceder a acciones de protección financiera y social mediante políticas dirigidas a esta población.

Abstract: Caring for a visually impaired child can affect the caregiver’s income and, in turn, the family’s. Catastrophic spending resulting from increased expenses and reduced income must be taken into account, whether due to unemployment, a reduction in the number of hours worked or the difficulty of entering or reentering the job market. Given this scenario, the main objective of this study was to estimate the catastrophic spending attributed to the caregiver of blind or low-vision children in reference centers for education for the blind, ophthalmology and child health located in the city of Rio de Janeiro, Brazil, identifying which factors are associated with a higher or lower prevalence of this expenditure. It was found that 53.3% of care for blind children involved 40% or more of their income. Among the caregivers of children with low vision, catastrophic spending is milder, compromising at least 40% of income for 36.8% of the caregivers. The factors associated with a higher prevalence of catastrophic spending were the caregiver’s age, the number of residents in the household, higher schooling, lower household income, renovations to the home, health insurance, taking out loans, selling assets, the number of health units where the child receives treatment and the relationship of the main caregiver. The burden placed on caregivers of visually impaired children indicates a situation of vulnerability that shows the need for access to financial and social protection mechanisms, through policies that are capable of serving this group.

https://doi.org/10.1590/0102-311xpt167723

Food environment of bus terminals in the Rio de Janeiro metropolitan region
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Jesus, Ana Carolina Castro de

; Botelho, Laís Vargas

; Canella, Daniela Silva

; Tavares, Letícia Ferreira

; Castro Junior, Paulo César Pereira de

; Silva, Isabela da Costa Gaspar da

; Cardoso, Letícia de Oliveira

Revista de Saúde Pública

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2024, Volume 58 elocation 04

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO OBJETIVO Descrever e analisar a saudabilidade dos estabelecimentos com venda formal e informal de alimentos em terminais rodoviários da região metropolitana do Rio de Janeiro. MÉTODOS Realizou-se auditoria em 156 estabelecimentos formais e 127 pontos informais de venda de alimentos localizados em 14 terminais rodoviários das cinco cidades mais populosas da região metropolitana do Rio de Janeiro. Foram calculadas proporções de tipos de estabelecimentos e médias (IC95%) de indicadores de disponibilidade de alimentos nos ambientes formal e informal. Para o ambiente formal, foram descritos preços, proporções das formas de pagamento aceitas, dias e horários de funcionamento e categorias de alimentos com propaganda exposta. RESULTADOS A saudabilidade dos pontos de venda de alimentos nos terminais rodoviários era baixa (inferior a 36%). Em média, estavam disponíveis para compra 250% mais subgrupos de alimentos ultraprocessados do que in natura ou minimamente processados. Adquirir comida nesses locais era conveniente porque diversas formas de pagamento estavam disponíveis e os horários de funcionamento dos estabelecimentos acompanhavam os picos de movimentação. Além disso, 73,3% das propagandas se referiam a bebidas ultraprocessadas e o custo-benefício da compra de alimentos ultraprocessados era melhor que o de alimentos in natura ou minimamente processados. CONCLUSÃO O ambiente alimentar dos terminais rodoviários da região metropolitana do Rio de Janeiro promove uma alimentação não saudável. Políticas públicas de regulação devem se concentrar em iniciativas que limitem a ampla disponibilidade e publicidade de alimentos ultraprocessados nesses espaços de grande circulação de pessoas.

ABSTRACT PURPOSE To describe and analyze the healthiness of formal and informal food establishments in bus terminals of the metropolitan region of the state of Rio de Janeiro. METHOD An audit was conducted in 156 formal and 127 informal food establishments located in 14 bus terminals of the five most populous cities of the metropolitan region of Rio de Janeiro. Proportions of types of establishments and means (95%CI) of food availability indicators in formal and informal settings were calculated. For the formal setting, prices, proportions of accepted payment methods, days and hours of operation, and food categories with displayed advertising were described. RESULTS The healthiness of food establishments in bus terminals was low (less than 36%). On average, ultra-processed food subgroups were 250% more available for purchase than fresh or minimally processed food. Purchasing food at these places was convenient because several forms of payment were available, and the opening hours of the establishments followed the peaks of movement. In addition, 73.3% of the advertising referred to ultra-processed drinks, and the cost-benefit of buying ultra-processed food was better than fresh or minimally processed food. CONCLUSION The food environment of bus terminals in the metropolitan region of Rio de Janeiro promotes unhealthy eating. Regulatory public policies should focus on initiatives to limit the wide availability and advertising of ultra-processed food in spaces of great circulation of people.

https://doi.org/10.11606/s1518-8787.2024058004769

Food environment of bus terminals in the Rio de Janeiro metropolitan region
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Jesus, Ana Carolina Castro de

; Botelho, Laís Vargas

; Canella, Daniela Silva

; Tavares, Letícia Ferreira

; Castro Junior, Paulo César Pereira de

; Silva, Isabela da Costa Gaspar da

; Cardoso, Letícia de Oliveira

Revista de Saúde Pública

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2024, Volume 58 elocation 04

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

https://doi.org/10.11606/s1518-8787.2024058004769

Electrocardiographic Findings Among Asymptomatic Adults With Haemophilia
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Camelo, Ricardo Mesquita

; Caram-Deelder, Camila

; Roncal, Carlos Guilherme Piscoya

; Duarte, Bruna Pontes

; Moura, Marilia Carolina Braga de

; Costa, Neuza Cavalcanti de Moraes

; Costa, Iris Maciel

; Vanderlei, Ana Maria

; Guimaraes, Tania Maria Rocha

; Gouw, Samantha

; Rezende, Suely Meireles

; Bom, Johanna van der

International Journal of Cardiovascular Sciences

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2024, Volume 37 elocation e20220179

Abstract: En | Text: En | PDF: En

Abstract Background: People with haemophilia (PwH) are living longer. Therefore, they can develop atherosclerotic cardiovascular disease (ASCVD). Electrocardiogram (ECG) alterations may be a sign of initial ASCVD before the occurrence of symptoms. Objective: To describe the prevalence of resting ECG alterations among PwH adults asymptomatic for ASCVD. Methods: PwH aged ≥ 30 years without previous ASCVD events were considered for the analysis. Resting ECG traces were analysed according to international reference values and the Brazilian Longitudinal Adult Health Study (ELSA-Brasil) results for asymptomatic Brazilian men. Based on the established normal values and using the QT index, we further described the altered ECGs as minor or major changes, according to the Minnesota Code. Differences between prevalences were evaluated by Pearson's χ2 test. Differences between medians were evaluated by the Mann-Whitney U test. A p-value < 0.05 was accepted as statistically significant. Results: A total of 64 PwH were included in the study. Median age was 44 years (interquartile range 35-52). Most patients had haemophilia A (81%) and 47% were severe. The prevalence of obesity, systemic arterial hypertension (SAH), diabetes mellitus (DM), and dyslipidaemia were 16%, 56%, 14%, and 72%, respectively. All the PwH had sinus rhythm, except for one, who had an implanted pacemaker due to idiopathic third-degree atrioventricular block. Altered ECGs were found in 25% and 30% of PwH, according to established criteria and ELSA-Brasil criteria, respectively. Major changes were found in eight (13%) PwH according to the Minnesota Code, including two ECGs with ischaemia-like wall inactivity. Conclusions: The prevalence of altered ECG varied from 25% to 30% among asymptomatic PwH. Background (PwH longer Therefore . (ASCVD) (ECG symptoms Objective Methods 3 analysis ELSABrasil ELSA Brasil (ELSA-Brasil men index Code Pearsons Pearson s χ test MannWhitney Mann Whitney pvalue p value 005 0 05 0.0 significant Results 6 study 4 interquartile 3552. 3552 35 52 35-52) 81% 81 (81% 47 severe obesity SAH, SAH , (SAH) DM, DM (DM) 16 16% 56 56% 14 14% 72 72% respectively rhythm one thirddegree third degree block 25 13% 13 (13% ischaemialike ischaemia like inactivity Conclusions (ASCVD 00 0. 355 5 35-52 8 (81 (SAH (DM 1 7 2 (13 35-5 (8 (1 35- (

https://doi.org/10.36660/ijcs.20220179

Gasto catastrófico em crianças com deficiência visual: estudo transversal com cuidadores no Rio de Janeiro, Brasil
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Barros, Letícia Baptista de Paula

; Zin, Andrea

; Moreira, Martha Cristina Nunes

; Santos, Marisa da Silva

; Costa, Ana Carolina Carioca da

; Campos, Daniel de Souza

; Neves, Luiza M.

; Haefeli, Lorena

; Entringer, Aline Piovezan

; Moreira, Maria Elisabeth Lopes

; Rebelo, Fernanda

; Vasconcelos, Zilton Farias Meira de

; Pinto, Márcia

Cadernos de Saúde Pública

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2024, Volume 40 N. 9 elocation e00167723

Abstract: Es Pt En | Text: Es Pt En | PDF: Es | PDF: Pt | PDF: En

https://doi.org/10.1590/0102-311xpt167723

Leishmania (Sauroleishmania) tarentolae versus pathogenic species: comparative evaluation of protease activity, glycoconjugates, resistance to complement and metabolome composition Sauroleishmania (Sauroleishmania species activity glycoconjugates
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Andrade, Filipe Fideles Duarte

; Vitório, Jéssica Gardone

; Canuto, Gisele André Baptista

; Nunes, Fernanda Freire Campos

; Rodrigues, Isabela Aurora

; Almeida, Ana Paula Martins Morais

; Nascimento, Frederico Crepaldi

; Costa, Adriana Oliveira

; Vieira, Tamara da Silva

; Silva, Ana Carolina Carvalho

; André, Leiliane Coelho

; Gontijo, Célia Maria Ferreira

; Junqueira, Caroline

; Toledo, Juliano Simões de

; Fernandes, Ana Paula

; Soares, Rodrigo Pedro

Memórias do Instituto Oswaldo Cruz

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2024, Volume 119 elocation e230243

Abstract: En | Text: En | PDF: En

BACKGROUND Leishmania tarentolae is a non-pathogenic species found in lizards representing an important model for Leishmania biology. However, several aspects of this Sauroleishmania remain unknown to explain its low level of virulence. OBJECTIVES We reported several aspects of L. tarentolae biology including glycoconjugates, proteolytic activities and metabolome composition in comparison to pathogenic species (Leishmania amazonensis, Leishmania braziliensis, Leishmania infantum and Leishmania major). METHODS Parasites were cultured for extraction and purification of lipophosphoglycan (LPG), immunofluorescence probing with anti-gp63 and resistance against complement. Parasite extracts were also tested for proteases activity and metabolome composition. FINDINGS Leishmania tarentolae does not express LPG on its surface. It expresses gp63 at lower levels compared to pathogenic species and, is highly sensitive to complement-mediated lysis. This species also lacks intracellular/extracellular activities of proteolytic enzymes. It has metabolic differences with pathogenic species, exhibiting a lower abundance of metabolites including ABC transporters, biosynthesis of unsaturated fatty acids and steroids, TCA cycle, glycine/serine/threonine metabolism, glyoxylate/dicarboxylate metabolism and pentose-phosphate pathways. MAIN CONCLUSIONS The non-pathogenic phenotype of L. tarentolae is associated with alterations in several biochemical and molecular features. This reinforces the need of comparative studies between pathogenic and non-pathogenic species to elucidate the molecular mechanisms of virulence during host-parasite interactions. nonpathogenic non However L glycoconjugates amazonensis braziliensis major. major . major) LPG, , (LPG) antigp63 antigp anti gp anti-gp6 complement surface gp6 complementmediated mediated lysis intracellularextracellular intracellular extracellular enzymes transporters steroids cycle glycineserinethreonine glycine serine threonine glyoxylatedicarboxylate glyoxylate dicarboxylate pentosephosphate pentose phosphate pathways features hostparasite host parasite interactions (LPG antigp6 anti-gp

https://doi.org/10.1590/0074-02760230243

Can habits and behaviors predict colonization by community-associated MRSA in patients admitted to a Brazilian hospital? communityassociated community associated hospital
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Pinheiro, Marcos Vinicius de Barros

; Cavalcante, Fernanda Sampaio

; Ferreira, Dennis de Carvalho

; Guimarães, Ana Carolina Fonseca

; Ferreira, Adriana Lúcia Pires

; Costa, Claudia Regina da

; Santos, Kátia Regina Netto dos

; Nouér, Simone Aranha

; Rangel, Ana Pereira

; Castiñeiras, Anna Carla

; Gonzalez, Christiany Moçali

; Freire, Joana

; Guimarães, Luiz Felipe

; Batista, Raquel

Revista do Instituto de Medicina Tropical de São Paulo

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2024, Volume 66 elocation e31

Abstract: En | Text: En | PDF: En

ABSTRACT This study aimed to identify factors associated with colonization by community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) in adult patients admitted to a Brazilian hospital. This is a cross-sectional study, in which patients underwent a nasal swab and were asked about hygiene behavior, habits, and clinical history. Among the 702 patients, 180 (25.6%) had S. aureus and 21 (2.9%) MRSA. The factors associated with MRSA colonization were attending a gym (OR 4.71; 95% CI; 1.42 – 15.06), smoking habit in the last year (OR 2.37; 95% CI; 0.88 – 6.38), previous hospitalization (OR 2.18; CI 95%; 0.89 – 5.25), and shared personal hygiene items (OR 1.99; 95% CI; 0.71 – 5.55). At the time of admission, colonization by CA-MRSA isolates was higher than that found in the general population. This can be an important public health problem, already endemic in hospitals, whose factors such as those associated with habits (smoking cigarettes) and behaviors (team sports practice and activities in gyms) have been strongly highlighted. These findings may help developing infection control policies, allowing targeting patients on higher-risk populations for MRSA colonization. communityassociated community methicillinresistant methicillin resistant CAMRSA CA (CA-MRSA hospital crosssectional cross sectional behavior history 70 18 25.6% 256 25 6 (25.6% S 2 2.9% 29 9 (2.9% OR 4.71 471 4 71 95 142 1 42 1.4 15.06, 1506 15.06 , 15 06 15.06) 2.37 237 37 088 0 88 0.8 6.38, 638 6.38 38 6.38) 2.18 218 089 89 5.25, 525 5.25 5 5.25) 1.99 199 99 071 0.7 5.55. 555 5.55 . 55 5.55) admission population problem hospitals cigarettes team gyms highlighted policies higherrisk risk 7 25.6 (25.6 2.9 (2.9 4.7 47 14 1. 150 15.0 2.3 23 3 08 8 0. 63 6.3 2.1 52 5.2 1.9 19 07 5.5 25. (25. 2. (2. 4. 15. 6. 5. (25 (2 (

https://doi.org/10.1590/s1678-9946202466031

10.

Factors associated with the absence of postpartum consultations in a high-risk population highrisk high risk
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Pereira, Ana Carolina Gomes

; Santos, Tábata Regina Zumpano dos

; Machado, Helymar da Costa

; Surita, Fernanda Garanhani de Castro

Revista Brasileira de Ginecologia e Obstetrícia

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2024, Volume 46 elocation e-rbgo23

Abstract: En | Text: En | PDF: En

Abstract Objective: To assess the rate of missed postpartum appointments at a referral center for high-risk pregnancy and compare puerperal women who did and did not attend these appointments to identify related factors. Methods: This was a retrospective cross-sectional study with all women scheduled for postpartum consultations at a high-risk obstetrics service in 2018. The variables selected to compare women were personal, obstetric, and perinatal. The variables of interest were obtained from the hospital's electronic medical records. Statistical analyses were performed using the Chi-square, Fisher's exact, or Mann–Whitney tests. For the variable of the interbirth interval, a receiver operating characteristic curve (ROC) was used to best discriminate whether or not patients attended the postpartum consultation. The significance level for the statistical tests was 5%. Results: A total of 1,629 women scheduled for postpartum consultations in 2018 were included. The rate of missing the postpartum consultation was 34.8%. A shorter interbirth interval (p = 0.039), previous use of psychoactive substances (p = 0.027), current or former smoking (p = 0.003), and multiparity (p < 0.001) were associated with non-attendance. Conclusion: This study showed a high rate of postpartum appointment non-attendance. This is particularly relevant because it was demonstrated in a high-risk obstetric service linked to clinical severity or social vulnerability cases. This highlights the need for new approaches to puerperal women before hospital discharge and new tools to increase adherence to postpartum consultations, especially for multiparous women. Objective highrisk risk factors Methods crosssectional cross sectional personal perinatal hospitals s records Chisquare, Chisquare Chi square, square Chi-square Fishers Fisher exact MannWhitney Mann Whitney ROC (ROC 5 5% Results 1629 1 629 1,62 201 included 348 34 8 34.8% p 0.039, 0039 0.039 , 0 039 0.039) 0.027, 0027 0.027 027 0.027) 0.003, 0003 0.003 003 0.003) 0.001 0001 001 nonattendance. nonattendance non attendance. attendance non-attendance Conclusion cases 162 62 1,6 20 3 34.8 0.03 03 002 0.02 02 000 0.00 00 16 6 1, 2 34. 0.0 0.

https://doi.org/10.61622/rbgo/2024rbgo23

11.

HRM practices in the public service: a measurement model service
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Demo, Gisela

; Costa, Ana Carolina Rezende

; Coura, Karla Veloso

RAUSP Management Journal

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2024, Volume 59 N. 1 Pages 50 - 66

Abstract: En | Text: En | PDF: En

Purpose Considering the significant increase in researchers’ interest in human resource management (HRM) in the public sector domain, this study aims to focus on producing a scale of HRM practices customized for the context of public organizations. Design/methodology/approach Experts and semantic analysis were performed for the scale development (qualitative stage), and exploratory and confirmatory factor analysis through structural equation modeling was conducted for the scale validation (quantitative stage). Findings The public HRM practices scale (public HRMPS) is composed of 19 items, distributed along four factors/dimensions, named training, development and education; relationship; work conditions; and competency and performance appraisal. The scale showed evidence of internal and construct validity (convergent, divergent, criterion-related and discriminant), as well as reliability and content validity. Research limitations/implications The public HRMPS can be applied in relational studies to test structural models of prediction, mediation and moderation to evaluate relationships with organizational behavior variables, such as leader-members exchange, engagement at work, life quality at work and well-being at work, among others. Practical implications The public HRMPS may also serve as a useful diagnostic tool for the decision-making process made by public managers so they can promote a strategic, evidence-based HRM. Furthermore, the transforming role of strategic HRM can be operationalized by adopting practices gathered in the public HRMPS, advancing toward new HRM strategies to promote healthier and more productive work environments. Social implications Healthier and more productive environments translate into real impacts for society, the first beneficiary of public services with more quality, efficiency and accountability. Originality/value The public HRMPS is the first attempt to produce an operationally valid and reliable measure to evaluate strategic HRM practices, responding to calls in the literature concerning the need for an integrated, comprehensive and customized HRM practices scale for the public service context. researchers (HRM domain organizations Designmethodologyapproach Design methodology approach qualitative stage, stage , stage) quantitative stage. . 1 items factorsdimensions factors dimensions factors/dimensions training education relationship conditions appraisal convergent, convergent (convergent divergent criterionrelated criterion related discriminant, discriminant discriminant) limitationsimplications limitations prediction variables leadermembers leader members exchange wellbeing being others decisionmaking decision making evidencebased based Furthermore society accountability Originalityvalue Originality value integrated

https://doi.org/10.1108/rausp-01-2023-0005

12.

Importance of genetic cancer risk assessment as a strategy to stratify risk and provide precision prevention in high-risk patients and families highrisk high
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Ashton-Prolla, Patricia

; Achatz, Maria Isabel Waddington

; Moreira, Miguel Angelo Martins

; Palmero, Edenir Inez

; Soares, Diogo Cordeiro de Queiroz

; Ferraz, Victor Evangelista de Faria

; Caires, Inacelli Queiroz de Souza

; Guindalini, Rodrigo Santa Cruz

; Gifoni, Ana Carolina Leite Vieira Costa

Revista da Associação Médica Brasileira

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2024, Volume 70 Suppl. 1 elocation e2024S117

Abstract: En | Text: En | PDF: En

https://doi.org/10.1590/1806-9282.2024s117

13.

The high biodiversity of benthic organisms in a coastal ecosystem revealed by an integrative approach
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Amaral, A. Cecília Z.

; Cunha, Beatriz P.

; Checon, Helio H.

; Godoy, Angélica S. de

; Silva, Camila F. da

; Corte, Guilherme N.

; Nogueira, João M. de M.

; Fukuda, Marcelo V.

; Steiner, Tatiana M.

; Kawauchi, Gisele Y.

; Turra, Alexander

; Denadai, Márcia R.

; Ferreira, Luciane

; Mendonça, Joel B. de

; Tavares, Marcos

; Leite, Fosca P. P.

; Costa, Mariana F. B.

; Siqueira, Silvana G. L.

; Vieira, Leandro M.

; Dias, Gustavo M.

; Teixeira, Joyce A.

; Rocha, Rosana M.

; Gusmão, Luciana C.

; Borges, Michela

; Alitto, Renata

; Machado, Fabrizio M.

; Passos, Flávio D.

; Cunha, Carlo M.

; Simone, Luiz R.L.

; Araujo, Ana Paula G.

; Carbayo, Fernando

; Bahia, Juliana

; Bulnes, Verónica N.

; Castello-Branco, Cristiana

; Hajdu, Eduardo

; Vilas-Boas, Ana Carolina

; Garraffoni, André R. S.

; Schockaert, Ernest

; Fonseca, Gustavo

; Domenico, Maikon Di

; Curini-Galletti, Marco

; Sørensen, Martin V.

; Hochberg, Rick

; Oliveira, Ana Julia F. C. de

; Zampieri, Bruna Del B.

; Chinelatto, Roberta M.

; Migotto, Alvaro E.

Biota Neotropica

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2024, Volume 24 N. 2 elocation e20231583

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Resumo O aumento da modificação dos habitats e da perda de espécies demanda esforços consistentes para descrever e compreender os padrões de biodiversidade. O programa BIOTA/FAPESP foi criado nesse contexto e é uma iniciativa de sucesso para promover estudos em biodiversidade e conservação no Brasil. O BIOTA/Araçá é um projeto interdisciplinar que promoveu uma avaliação detalhada da biodiversidade da Baía do Araçá, um ecossistema costeiro localizado ao Norte do estado de São Paulo, Sudeste do Brasil. A baía engloba múltiplos habitats, tais como praias, manguezais, costões rochosos, e uma planície de maré, e também fornece importantes serviços ecossistêmicos. Infelizmente, a baía está sujeita à conflitos sócio-ambientais complexos que contrastam demandas econômicas, sociais e ambientais (i.e. a expansão das atividades do porto vizinho vs. a pesca artesanal de pequena escala e a proteção da biodiversidade). O presente estudo apresenta um levantamento das espécies bentônicas que ocorrem nos diferentes habitats da Baía do Araçá, incluindo dados obtidos durante o projeto BIOTA/Araçá e de investigações realizadas anteriormente na área. As espécies bentônicas desempenham um papel importante no ambiente marinho, e estudar a diversidade desses organismos que vivem associados ao fundo é indispensável para compreender o funcionamento do meio ambiente. A macrofauna, meiofauna, e microorganismos associados aos fundos consolidado e inconsolidado foram listados, e informações adicionais foram fornecidas para cada espécie, tais como a distribuição geográfica e nos habitats. O checklist inclui 826 espécies, quase 70% registradas durante o projeto BIOTA/Araçá. Os taxa mais especiosos foram os anelídeos (225 spp.), moluscos (194 spp.), e crustáceos (177 spp.). Entre as espécies bentônicas listadas, sete são endêmicas da Baía do Araçá, 14 são consideradas ameaçadas de extinção, e sete são exploradas economicamente. A baía é a localidade tipo de vários taxa, e 11 novas espécies bentônicas foram descritas com base em espécimes amostrados durante o projeto. Este projeto mostra a importância da Baía do Araçá como um ambiente de riqueza biológica única e demonstra a necessidade de esforços para a sua conservação considerando as atuais ameaças. BIOTAFAPESP BIOTA FAPESP Brasil BIOTAAraçá Paulo praias manguezais rochosos maré ecossistêmicos Infelizmente sócioambientais sócio econômicas i.e. ie i (i.e vs . biodiversidade) área marinho macrofauna meiofauna listados espécie 82 70 225 (22 spp., spp spp. , spp.) 194 (19 177 (17 spp.. listadas 1 extinção economicamente ameaças i.e 8 7 22 (2 19 (1 17 2 (

Abstract Increasing habitat modification and species loss demand consistent efforts to describe and understand biodiversity patterns. The BIOTA/FAPESP Program was created in this context and it has been a successful initiative to promote studies on biodiversity and conservation in Brazil. The BIOTA/Araçá is an interdisciplinary project that provided a detailed evaluation of the biodiversity of Araçá Bay, a coastal seascape located on the North coast of the state of São Paulo, Southeast Brazil. The bay encompasses multiple habitats, such as beaches, mangroves, rocky shores, and a tidal flat, and provides important ecosystem services. Unfortunately, the bay is the subject of complex social-environmental conflicts that oppose economic, social, and environmental demands (i.e., the expansion of neighboring harbor activities vs. small-scale artisanal fisheries and protection of biodiversity). The present study presents a survey of the benthic species occurring in the different habitats of Araçá Bay, including data obtained during the BIOTA/Araçá project and previous assessments of the area. The benthic species play an important role in marine environments and studying the diversity of these organisms that live associated with the bottom is indispensable for comprehending the environment’s functioning. The macrofauna, meiofauna, and microorganisms associated with soft and hard bottom were listed, and additional information, such as the habitat and geographical distribution, were provided for each species. The checklist includes 826 species, almost 70% recorded during the BIOTA/Araçá project. The most speciose taxa were the annelids (225 spp.), mollusks (194 spp.), and crustaceans (177 spp.). Seven benthic species are endemic to Araçá Bay, 14 are considered threatened, and seven are economically exploited. Furthermore, the bay is the type locality of many taxa, and 11 new benthic species were described based on specimens sampled during the project. This project shows the importance of Araçá Bay as a unique biologically rich environment and highlights the need for conservation efforts in light of the current threats. patterns BIOTAFAPESP BIOTA FAPESP Brazil BIOTAAraçá Paulo beaches mangroves shores flat services Unfortunately socialenvironmental social economic i.e., ie i e (i.e. vs smallscale small scale biodiversity. . biodiversity) area s functioning macrofauna meiofauna listed information distribution 82 70 225 (22 spp., spp spp. , spp.) 194 (19 177 (17 spp.. 1 threatened exploited Furthermore threats i.e. (i.e 8 7 22 (2 19 (1 17 i.e 2 (

https://doi.org/10.1590/1676-0611-bn-2023-1583

14.

Neurological manifestation of Brazilian spotted fever in childhood
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Mendes, Bruna Fernanda Deicke

; Moreira, Marina Melo

; Jimenez, Ana Luisa Lodi

; Silva, Lívia Barbosa da

; Thiersch, Laura Maria Silva

; Rodrigues, Carolina Malaquias

; Torres, Bruna Ribeiro

; Costa, Juliana Goulart Dias da

; Diniz, Lilian Martins Oliveira

Revista do Instituto de Medicina Tropical de São Paulo

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2024, Volume 66 elocation e16

Abstract: En | Text: En | PDF: En

ABSTRACT Rocky Mountain Spotted Fever is a rickettsial disease caused by the bacteria Rickettsia rickettsii. In Brazil, the disease is known as Brazilian spotted fever (BSF), being the most significant tick-borne disease in the country. Among the affected patients, only 5% of cases occur in children aged one to nine years. Typical symptoms of the disease are fever, rash, headache and digestive symptoms. Neurological manifestations such as seizures, aphasia and hemiparesis have been described in few patients. This study aimed to describe the case of an infant diagnosed with BSF who presented severe signs of neurological manifestation. rickettsii Brazil BSF, , (BSF) tickborne tick borne country patients 5 years rash seizures manifestation (BSF

https://doi.org/10.1590/s1678-9946202466016

15.

All-polymer-based ammonia gas sensor: applying insights from the morphology-driven ac electrical performance Allpolymerbased All polymer based sensor morphologydriven morphology driven
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Kelmer, Ana Carolina

; Costa, Cleidinéia Cavalcante da

; Bianchi, Rodrigo Fernando

Polímeros

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2024, Volume 34 N. 1 elocation e20240012

Abstract: En | Text: En | PDF: En

Abstract This paper investigates the electrical, morphological, and mechanical behavior of ultrathin layer-by-layer polyaniline/poly(vinyl sulfonic acid) (PANI/PVS) ultrathin films for ammonia gas sensing. Atomic force microscopy shows that the PANI/PVS surface's roughness increases almost linearly with the number of PANI/PVS bilayers, while the surface morphology varies from a rod-like structure to a film-like architecture. Impedance measurements and their representation by a Cole-Cole model confirm this transition at ~15 bilayers. The designed sensor shows low response time (< 1 min), an optimal operating frequency range (1–100 Hz), high stability and sensibility to ammonia (~ 98 kΩ/ppm), and low sensibility to strain (~ 3.6 kΩ/%). This study suggests that hopping carriers' concentration remains constant, and hopping carriers' mobility changes with the number of bilayers. The simultaneous analysis of morphology with complex impedance measurements is a strategy for enhancing the electrical performance of low-cost and flexible organic sensing devices. morphological layerbylayer layer polyaniline/polyvinyl polyanilinepolyvinyl polyaniline/poly vinyl polyaniline poly acid PANIPVS PANI PVS (PANI/PVS surfaces s bilayers rodlike rod like filmlike film architecture ColeCole Cole 15 ~1 < ( min, min , min) 1–100 1100 100 (1–10 Hz, Hz Hz) ~ 9 kΩ/ppm, kΩppm kΩ/ppm kΩ ppm kΩ/ppm) 36 3 6 3. kΩ/%. kΩ/% . kΩ/%) carriers constant lowcost cost devices polyvinyl polyanilinepoly 1–10 110 10 (1–1 kΩ/ 1–1 11 (1– 1– (1

https://doi.org/10.1590/0104-1428.20230070

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