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Resumen Antecedentes: El cáncer de células pequeñas (CPCP) representa el 13-15% del total de neoplasias primarias de pulmón. Se caracteriza por su rapidez en la tasa de crecimiento y en el desarrollo de metástasis a distancia. Objetivos: Orientar y estandarizar el tratamiento del CPCP, enfermedad limitada, en México, basado en evidencia clínica nacional e internacional. Material y métodos: Este documento se desarrolló como una colaboración entre el Instituto Nacional de Cancerología y la Sociedad Mexicana de Oncología en cumplimiento con estándares internacionales. Se integró un grupo conformado por oncólogos médicos, cirujanos oncólogos, cirujanos de tórax, radio-oncólogos y metodólogos con experiencia en revisiones sistemáticas de la literatura y guías de práctica clínica. Resultados: Se consensuaron, por el método Delphi y en reuniones a distancia, las recomendaciones en CPCP enfermedad limitada, producto de las preguntas de trabajo. Se identificó y evaluó críticamente la evidencia científica que responde a cada una de dichas preguntas clínicas, antes de incorporarla a la guía. Conclusión: Esta guía proporciona recomendaciones clínicas para el manejo de la enfermedad limitada del CPCP y durante el proceso de toma de decisiones de los clínicos involucrados con su manejo en nuestro país para mejorar la calidad de la atención clínica en estos pacientes.

Abstract Background: Small cell lung cancer (SCLC) represents 13-15% of all primary lung neoplasms and is characterized by rapid growth rate and development of distant metastases. Objectives: To guide and standardize the treatment of limited disease small cell lung cancer in Mexico based on national and international clinical evidence. Material and methods: This document was developed as a collaboration between the National Cancer Institute and the Mexican Society of Oncology in compliance with international standards. An interdisciplinary group was formed, including medical oncologists, oncological surgeons, thoracic surgeons, radiation oncologists, and methodologists with experience in systematic reviews of the literature and clinical practice guidelines. Results: Consensus recommendations were reached, both by the Delphi method and in remote meetings, for limited SCLC disease resulting from the same number of work questions. The scientific evidence that answers each of these clinical questions was identified and critically evaluated, before being incorporated into the body of evidence of the guide. Conclusion: This guide provides clinical recommendations for the management of limited disease SCLC to contribute to the decision-making process of the clinicians involved with its management in our country, hoping that this will contribute to improving the quality of clinical care in these patients.

https://doi.org/10.24875/j.gamo.22000121

The Baecke Habitual Physical Activity Questionnaire (BHPAQ): a valid internal structure of the instrument to assess healthy Brazilian adults
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Rocha, Daniel Santos

; Dibai-Filho, Almir Vieira

; Pinheiro, Jocassia Silva

; Azevedo, Lisiane Fernanda Simeão de

; Falcai, Angela

; Tavarez, Rudys Rodolfo de Jesus

; Rego, Adriana Sousa

; Santana, Gabriela Nascimento de

; Gomes, Cid André Fidelis de Paula

; Bassi-Dibai, Daniela

Revista da Associação Médica Brasileira

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jul 2022, Volume 68 N. 7 Pages 912 - 916

Abstract: En | Text: En | PDF: En

SUMMARY OBJECTIVE: This study aimed to validate the internal structure of the Brazilian version of the Baecke Habitual Physical Activity Questionnaire. METHODS: A cross-sectional study was conducted with individuals over 18 years old of both sexes, with Brazilian Portuguese as their native language. The structure of the Baecke Habitual Physical Activity Questionnaire was tested by confirmatory factor analysis. The model fit was evaluated by the following indices: root mean square error of approximation, comparative fit index, Tucker-Lewis index, standardized root mean square residual, and χ²/degrees of freedom. We used the Akaike information criterion and Bayesian information criterion to compare different structures of the Baecke Habitual Physical Activity Questionnaire. RESULTS: A total of 241 individuals participated in this study. The original structure of the Baecke Habitual Physical Activity Questionnaire with 16 items and 3 domains was compared to a structure with 14 items and 3 domains. The internal structure of the Baecke Habitual Physical Activity Questionnaire with 14 items showed better fit indices and lower Akaike information criterion and Bayesian information criterion values. CONCLUSION: The best internal structure of the Brazilian version of the Baecke Habitual Physical Activity Questionnaire in adults presents 3 domains and 14 items.

https://doi.org/10.1590/1806-9282.20211374

SFRP1, Posible biomarcador en la progresión o regresión de lesiones de cérvix asociado al Virus del Papiloma Humano
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Baena-Acevedo, Juvenal Darío

; García-Robayo, Dabeiba Adriana

; Cid-Arregui, Ángel

; Aristizábal-Gutiérrez, Fabio

; Castañeda-Peláez, Diego Andrés

Infectio

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dec 2021, Volume 25 N. 4 Pages 270 - 275

Abstract: Es En | Text: Es En | PDF: Es | PDF: En

Resumen Objetivo: Comparar la expresión de mRNA y proteínas de SFRP1, PTPRN, CDO1, EDNRB, CDX2, EPB41L3 y HAND1 en pacientes con lesión intra-epitelial cervical de bajo y alto grado, con posterior progresión o regresión. Material y Método: Se realizó análisis de expresión de genes mediante RT-PCR y análisis de expresión de proteínas por inmunohistoquímica. El análisis estadís tico fue realizado con las pruebas: Wilcoxon, coeficiente de correlación de Spearman e índice de concordancia. Las muestras fueron pareadas en momento 1 y momento 2. Resultados: SFRP1 mostró tendencia de mayor expresión de mRNA en lesión intra-epitelial de bajo grado (momento 2) Vs. alto grado (momento 1). La expresión de proteínas por inmunohistoquímica de SFRP1 en casos de progresión (83,3 %) mostró disminución en su graduación (p = 0,0313*); los demás genes en estudio no tuvieron cambios estadísticamente significativos. Discusión: SFRP1 mostró comportamiento ajustado a resultados de estudios previos donde se encontró hipermetilado en lesiones intra-epiteliales de alto grado; su subexpresión por hipermetilación se reportó en otros canceres, proceso que colabora con su silenciamiento y transición epitelial-mesenquimatosa del cáncer de cuello uterino. Conclusiones. SFRP1 es potencial biomarcador en lesiones preneoplásicas del cuello uterino asociadas al virus de papiloma humano.

Abstract Objective. The aim of this work was to compare the expression of mRNA and proteins of SFRP1, PTPRN, CDO1, EDNRB, CDX2, EPB41L3 and HAND1 in patients with low and high grade cervical intraepithelial lesion, with subsequent progression or regression. Material and Methods: Gene expression analysis was conducted through RT-PCR and protein expression analysis was performed by immunohistochemistry. The statistics analysis were Wilcoxon test, Spearman’s correlation coefficient and concordance index. The samples were paired during moment 1 (initial patient diag nosis) and moment 2 (follow-up histological diagnosis). Results: SFRP1 showed a trend of higher mRNA expression in low-grade intra-epithelial lesions (moment 2) Vs. high-grade (moment 1). The expression of proteins by immunohistochemistry of SFRP1 in progression cases (83.3%) showed a decrease in its graduation (p = 0.0313*); the other genes under study had no statistically significant. Discussion: SFRP1 showed a biological behavior adjusted to the results of previous studies where hypermethylation was found in high-grade intra-epithelial lesions; its subexpression by hypermethylation has been reported in other cancers, a process that collaborates with its silencing and epithelial-mesenchymal tran sition of cervical. Conclusions. SFRP1 is a potential biomarker in preneoplastic lesions of the cervix associated with human papillomavirus.

https://doi.org/10.22354/in.v25i4.959 235 downloads

Brazilian version of the Self-Estimated Functional Inability because of Pain questionnaire for musculoskeletal injuries relating to dance and sport: translation and cross-cultural adaptation
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Reis-Júnior, Jodimar Ribeiro

; Protázio, Jhonata Botelho

; Muribeca-de-Castro, Aila Maria

; Pinheiro, Jocassia Silva

; Takahasi, Henrique Yuji

; Pires, Flavio de Oliveira

; Souza, Sergio Augusto Rosa de

; Fidelis-de-Paula-Gomes, Cid André

; Rêgo, Adriana Sousa

; Bassi-Dibai, Daniela

; Dibai-Filho, Almir Vieira

Sao Paulo Medical Journal

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feb 2020, Volume 138 N. 1 Pages 11 - 18

Abstract: En | Text: En | PDF: En

ABSTRACT BACKGROUND: Self-Estimated Functional Inability because of Pain (SEFIP) is a questionnaire specifically designed to measure musculoskeletal pain or discomfort. OBJECTIVE: To perform translation and cross-cultural adaptation of the SEFIP for dancers (SEFIP-dance), for use in Brazilian Portuguese. In addition, as a secondary objective, we adapted the translated version of SEFIP-dance for use among athletes or exercise practitioners (SEFIP-sport). DESIGN AND SETTING: Questionnaire translation and cross-cultural adaptation study conducted at a public university. METHODS: The Brazilian version of the SEFIP-dance questionnaire was developed following the processes of translation (involving two translators with Brazilian Portuguese as their mother tongue and fluency in English), backtranslation (involving two translators with English as their mother tongue and fluency in Brazilian Portuguese), committee review and pre-testing. SEFIP-sport was developed following the processes of content and face validation. RESULTS: SEFIP-dance was applied to 30 dancers, of mean age 22.38 years (standard deviation [SD] = 3.41), among whom 14 were men (46.66%). The participants understood 100% of the SEFIP-dance items and alternatives. SEFIP-sport was applied to 30 athletes or physical exercise practitioners, of mean age 25.09 years (SD = 8.93), among whom 25 were men (86.33%). The participants understood 100% of the SEFIP-sport items and alternatives. CONCLUSION: The Brazilian Portuguese versions of SEFIP-dance, translated and cross-culturally adapted for dancers, and SEFIP-sport, adapted for athletes or physical exercise practitioners, were shown to have adequate levels of understanding.

https://doi.org/10.1590/1516-3180.2019.0375.r1.08102019 765 downloads

Factors influencing occupational accidents: a multidimensional analysis in the electricity sector
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Alves, Adriana Maria Silva

; Gonçalves Filho, Cid

; Santos, Nathália de Melo

; Souki, Gustavo Quiroga

Gestão & Produção

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2020, Volume 27 N. 2 elocation e4609

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Resumo: Os acidentes de trabalho são um problema de saúde pública e, por isso, faz-se necessária a condução de pesquisas que auxiliem na prevenção de acidentes e na promoção da saúde. O objetivo deste trabalho foi investigar os principais fatores que influenciam os acidentes de trabalho em uma concessionária de energia elétrica, visando auxiliar na prevenção de acidentes. Para alcançar o objetivo proposto, foi realizada uma pesquisa com 1.027 empregados próprios ou terceirizados de uma grande empresa do setor elétrico, abrangendo profissionais acidentados e não acidentados. Os resultados obtidos demonstram que fatores organizacionais, pessoais/comportamentais e do trabalho/tarefa apresentam impactos estatisticamente significativos sobre os acidentes de trabalho. Como contribuição acadêmica e gerencial, a identificação dos principais fatores que influenciam os acidentes de trabalho no setor elétrico favorece o desenvolvimento de estratégias e ações para o seu controle e mitigação.

Abstract: Occupational accidents are a public health problem; therefore, it is necessary to conduct research that contributes to accident prevention and health promotion. To this end, this study aimed to investigate the main factors influencing work accidents at an electric power company. A survey of 1,027 in-house and outsourced employees of a large electric utility was conducted. The participants included injured and non-injured professionals. Organizational, personal/behavioral, and work/task factors were found to have statistically significant effects on work accident occurrence. As an academic and managerial contribution, the identification of the main factors that influence work accidents in the electric sector favors the development of strategies and actions for its control and mitigation.

https://doi.org/10.1590/0104-530x4609-20 1485 downloads

Expresión génica de ligandos mica, micb y ulbp (1-6) del receptor NKG2D de células natural killer y metaloproteinasas adam10, adam17 y mmp14 en lineas celulares de cáncer de cervical
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García, Dabeiba Adriana

; Pérez, Pilar

; García, Laura

; Cid-Arregui, Angel

; Aristizabal, Fabio

Revista Colombiana de Biotecnología

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jun 2019, Volume 21 N. 1 Pages 29 - 38

Abstract: Es En | Text: Es En | PDF: Es | PDF: En

RESUMEN El CCU es la segunda causa de muerte en mujeres de nuestro país. Dentro de los primeros mecanismos de defensa del hospedero se encuentra la respuesta inmune de las células NK y su función lítica a expensas de su receptor activador NKG2D, el cual posee como ligandos mica, micb y ulbp (1-6), los cuales se expresan en células transformadas y/o infectadas por virus. Uno de los mecanismos de evasión por parte de la célula tumoral es el clivaje de estas proteínas a través de metaloproteinasas como adam10, adam17 y mmp14. Se analizó la expresión de estos ligandos y metaloproteinasas mediante PCR tiempo real, en lineas celulares de referencia para cáncer cervical como HeLa (positiva para VPH-18) y C33A (negativa para VPH). Se obtuvieron valores representativos de expresion relativa genica con diferencias significativas asi: mmp14 en linea HeLa (p= 0.006); y mica y ulbp-3 en la linea C33A (p= 0.020 y p=0.003 respectivamente). Por lo tanto, se podría sugerir que la expresión de mmp14 se encuentra posiblemente involucrados con la presencia de VPH causante del cancer cervical y la respuesta inmunne innata desarrollada.

ABSTRACT Cervical cancer is the second leading cause of death in women in our country. Within the first host defense mechanisms is the immune response of NK cells and their lytic function at the expense of its NKG2D receptor activator which has as ligands mica, micb and ulbp (1-6), which are expressed in transformed cells and / or virally infected. One of the mechanisms of evasion by the tumor cell is the cleavage of these proteins through metalloproteinases as adam10, adam17 and mmp14. We analyzed the expression of these ligands and metalloproteinases by real time PCR, in reference to cell lines HeLa cervical cancer (positive for HPV-18) and C33A (negative for HPV). We obtained representing relative gene expression with significant differences from the other lines of study as follows: mmp14 in HeLa (p = 0.006); and mica and ulbp-3 in C33A (p = 0.020 and p = 0.003 respectively). Thus one might suggest that the expression of mmp14 is possible involved with HPV presence causing high risk of cervical cancer and innate inmunne response developed.

https://doi.org/10.15446/rev.colomb.biote.v21n1.79730 750 downloads

Expresión de EDNRB y CDX2 posibles biomarcadores en progresión al cáncer cervical
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Baena, Juvenal

; Castañeda, Diego Andres

; Cid-Arregui, Angel

; Aristízabal, Fabio

; García, Dabeiba Adriana

Revista Colombiana de Biotecnología

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jun 2018, Volume 20 N. 1 Pages 6 - 15

Abstract: Es En | Text: Es En | PDF: Es | PDF: En

RESUMEN De acuerdo a la historia natural del cáncer del cuello uterino, en donde las lesiones preneoplásicas de bajo y alto grado pueden presentar fenómenos de regresión o progresión, existe gran interés en la búsqueda de biomarcadores que permita predecir la evolución de las lesiones preneoplásicas del cérvix hacia la progresión o regresión de la enfermedad. Estos biomarcadores pudieran ser de origen genético, o epigenético que alteren la expresión de los genes y que pudieran estar asociados con la carcinogénesis en diferentes tipos de tejido humano. El objetivo del estudio fue analizar la expresión del mARN de los genes SFRP1, PTPRN, CDO1, EDNRB, CDX2, EPB41L3 y HAND1 en muestras negativas para lesiones intraepiteliales cervicales (n=9), muestras con lesiones intraepiteliales de bajo grado (n=10) y alto grado (n=11). Se realizó análisis de expresión de los genes mencionados mediante qRT-PCR y el análisis de los datos se realizó mediante la prueba no paramétrica de ANOVA. La diferencia estadística se determinó en valores p< 0,05. Para los genes EDNRB y CDX2 se observó disminución 66,7% en las muestras sin alteraciones histológicas cervicales, comparado con una disminución en la expresión del 50% en muestras con LIEBG y para el grupo de LIEAG del 36,4% para el gen EDNRB y del 27,3% para el gen CDX2 dando una diferencia estadísticamente significativa p= 0,02. Sugiriendo que EDNRB y CDX2 podrían ser útiles como posibles biomarcadores en la carcinogénesis cervical.

ABSTRACT According into account the natural history of cervical cancer, where low- and high-grade preneoplastic lesions may present regression or progression phenomena, there is great interest in the search for biomarkers to predict the behavior of preneoplastic lesions of the cervix. These biomarkers may be of genetic origin, or epigenetics that alter the expression of genes and that may be associated with carcinogenesis in different types of human tissue. The objective of the study was to analyze the expression of the mRNA of the SFRP1, PTPRN, CDO1, EDNRB, CDX2, EPB41L3 and HAND1 genes in samples negative for cervical intraepithelial lesions (n = 9), low grade intraepithelial lesions (n=10) and high grade (n = 11). Expression analysis of the mentioned genes was performed using qRT-PCR and data analysis was performed using the non-parametric ANOVA test. The statistical difference was determined in values p <0.05. For the EDNRB and CDX2 genes, a 66.7% decrease was observed in the samples without cervical histological alterations, compared to a decrease in expression of 50% in LIEBG samples and 36.4% in the LIEAG group for the EDNRB gene And 27.3% for the CDX2 gene giving a statistically significant difference p = 0.02. Suggesting that EDNRB and CDX2 could be useful as potential biomarkers in cervical carcinogenesis.

https://doi.org/10.15446/rev.colomb.biote.v20n1.64114 626 downloads

Crecimiento, rendimiento y rentabilidad del maíz VS-535 en función del biofertilizante y nitrógeno
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Aguilar-Carpio, Cid

; Escalante-Estrada, José Alberto Salvador

; Aguilar-Mariscal, Immer

; Pérez-Ramírez, Adriana

Ecosistemas y recursos agropecuarios

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dec 2017, Volume 4 N. 12 Pages 475 - 483

Abstract: En Es | Text: En Es | PDF: En | PDF: Es

Abstract: The objective of the study was to determine the effect of biofertilizer and nitrogen on the synthetic variety of VS-535 corn, on the occurrence of phenological phases, growth analysis, performance, components, wáter use effciency, nitrogen content and profitability. The sowing was performed on July 10, 2012, the treatments were the inoculation of biofertilizer and the application of 0, 80, and 160 kg ha-1 of nitrogen. The number of leaves and leaf area were counted, calculating the index (LAI) and leaf area duration (TLAD). The dry matter (DM) content was also determined, as well as the crop growth rate and the net assimilation rate was also evaluated. On physiological maturity, the dry matter (DM) content (DM) and grain performance (GP) were evaluated; the effciency in the use of water and nitrogen was calculated, and an economic analysis was performed. It had the highest LAI and TLAD with 160 kg ha-1 of nitrogen.For DM, EUA, EAN, , while the older were with biofertilizer and nitrogen at 80 and 160 kg ha-1.For GP, the highest response was found with biofertilizer and nitrogen at 160 kg ha-1, which generated the highest profitability of the crop.

Resumen: El objetivo del estudio fue determinar el efecto del biofertilizante y del nitrógeno en la variedad sintética de maíz VS-535 sobre la ocurrencia de las fases fenológicas, análisis de crecimiento, rendimiento, componentes, eficiencia en el uso del agua, contenido de nitrógeno y la rentabilidad. La siembra se realizó el 10 de julio de 2012, los tratamientos fueron la inoculación de biofertilizante y la aplicación de 0, 80 y 160 kg ha-1 de nitrógeno. Se contabilizó el número de hojas y el área foliar, con lo que se calculó el índice (IAF) y la duración de área foliar (DAFT). También se determinó el contenido de materia seca (MS), las tasa de crecimiento del cultivo , y la tasa de asimilación neta . En madurez fisiológica se evaluó el contenido de materia seca (MS) y el rendimiento de grano (RG), se calculó la eficiencia en el uso del agua y del nitrógeno, y se realizó un análisis económico. Con 160 kg ha-1 de nitrógeno se tuvo el mayor IAF y DAFT. Para MS, EUA, EAN, , mientras que las mayores se tuvieron con biofertilizante y nitrógeno a 80 y 160 kg ha-1. Para RG la mayor respuesta se encontró con biofertilizante y nitrógeno a 160 kg ha-1, lo que genero la mayor rentabilidad del cultivo.

https://doi.org/10.19136/era.a4n12.1000 1578 downloads

Combate químico de Thrips tabaci (Thysanoptera: Thripidae) en el cultivo de cebolla en Morelos, México
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Aguilar Carpio, Cid

; González Rendón, Arturo

; Pérez Ramírez, Adriana

; Ramírez Rojas, Sergio Gavino

; Carapia Ruiz, Vicente Emilio

Acta zoológica mexicana

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apr 2017, Volume 33 N. 1 Pages 39 - 44

Abstract: En Es | Text: En Es | PDF: En | PDF: Es

Abstract: The onion crop has high populations of thrips (Thrips tabaci Lindeman), which causes premature wilting, retard leaf development and distorts vegetative buds. Farmers have been used different pesticides for managing trips, regardless of the rotation of chemical groups and inadequate doses, causing environmental pollution and overuse of agrochemicals. The objective of this study was to evaluate six groups of pesticides for the management of thrips, which were rotated during the growing season and the thrips species present in an onion crop also was identified. The study began in October 2013 in an onion crop, located in Ayala, Morelos. Each treatment consisted of the application of the following insecticides: T1: Control (no products); T2: Movento, Velcefate, Muralla, Tracer; T3: Regent, Muralla, Movento, Seizer+ Agromectin; T4 Tracer, Movento, Curacron, Muralla; T5 Actigard, Tracer, Colt, Agromectin; T6 Evisect-s, Curacron, Tracer, Calypso. To determine control of insecticides, the number of nymphs and adult thrips were counted. Analysis of variance and mean separation test with Tukey (α 0.05) was performed with data on the number of nymphs and adult thrips. To determine the species of thrips present, a sample was taken from the leaves of onion plants. Insecticides that achieve control of nymphs of thrips in onion crop were the Tracer, Acephate, Curacron and combining Seizer + Agromectin. The Tracer and Muralla insecticides were those who maintained the lowest adult thrips in onion growing populations. The predominant species of thrips in onion crop during the study was Thrips tabaci L.

Resumen: El cultivo de cebolla presenta altas poblaciones del trips (Thrips tabaci Lindeman), lo que ocasiona marchitez prematura, retarda el desarrollo de la hoja y distorsiona los brotes vegetativos. Los agricultores han usado diferentes plaguicidas para el manejo del trips, sin tener en cuenta la rotación de grupos químicos y dosis adecuadas, lo que ocasiona contaminación ambiental por el uso excesivo de agroquímicos. Por lo que el objetivo de este estudio, fue evaluar seis grupos de plaguicidas para el manejo de trips, los cuales fueron rotados durante el ciclo del cultivo, así como también se identificó la especie de trips presente en el cultivo de la cebolla. El estudio inició en octubre de 2013, en un cultivo de cebolla, ubicada en Ayala, Morelos. Cada tratamiento consistió en la aplicación de los siguientes insecticidas: T1: Testigo (sin producto); T2: Movento, Velcefate, Muralla, Tracer; T3: Regent, Muralla, Movento, Seizer+ Agromectin; T4: Tracer, Movento, Curacron, Muralla; T5: Actigard, Tracer, Colt, Agromectin; T6: Evisect-s, Curacron, Tracer, Calypso. Para determinar el control de los insecticidas, se contabilizó el número de ninfas y adultos de trips. Con los datos del número de ninfas y adultos de trips en los diferentes tratamientos, se realizó un análisis de varianza y separación de medias con la prueba Tukey (α 0.05). Para determinar las especies de trips presentes, se tomó una muestra en las hojas de las plantas de cebolla. Los insecticidas que lograron un control de las ninfas de trips en el cultivo de cebolla, fueron el Tracer, Acefate, Curacron y la combinación de Seizer+ Agromectin. Los insecticidas Tracer y Muralla, fueron los que mantuvieron las poblaciones más bajas de adultos de trips en el cultivo de cebolla. La especie de trips predominante en el cultivo de cebolla durante el estudio fue Thrips tabaci L.

15056 downloads

10.

Eugeniamyia dispar em pitangueira: parasitoides associados, dinâmica populacional e distribuição de galhas na planta
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Bierhals, Adriana Neutzling

; Nava, Dori Edson

; Costa, Valmir Antonio

; Maia, Valéria Cid

; Diez-Rodríguez, Gabriela Inés

Revista Brasileira de Fruticultura

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mar 2012, Volume 34 N. 1 Pages 109 - 115

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

A pitangueira Eugenia uniflora L. (Myrtaceae) é uma frutífera nativa da América do Sul, podendo ser encontrada em quase todo o território brasileiro, bem como no Paraguai, no Uruguai e na Argentina. Nas folhas dessa planta, são encontradas galhas induzidas por Eugeniamyia dispar (Diptera: Cecidomyiidae). O objetivo deste trabalho foi determinar os parasitoides associados a E. dispar, a dinâmica populacional de E. dispar e dos parasitoides e a distribuição de galhas na planta. Ramos e folhas foram coletados quinzenalmente, durante as safras agrícolas de 2007/2008 e 2008/2009, em pomares de pitangueira localizados no município de Pelotas-RS, e transportados para o laboratório para a contagem do número de galhas. Do material coletado, foram individualizadas dez folhas em recipientes plásticos (200 mL) contendo 30 mL de uma solução à base de ágar-água (2%) e metilpara-hidroxibenzoato (nipagin) (0,2%). Os recipientes foram fechados e mantidos sob condições controladas de temperatura (24±2ºC), umidade relativa (70±10%) e fotofase (14h), sendo registrado, diariamente, o número de insetos emergidos. Obteve-se uma espécie de Hymenoptera, Rileya hegeli (Eurytomidae), como parasitoide de E. dispar. Quanto à dinâmica populacional de E. dispar e de R. hegeli, foi observado, nos dois anos de avaliação, que a população de ambos aumentou a partir de outubro, atingindo as maiores populações nos meses de dezembro, janeiro e fevereiro, decrescendo posteriormente. O número de galhas por folha variou de zero a 23, sendo que 61,19% das folhas apresentavam de uma a oito galhas e que o número de folhas com galhas por ramo variou de uma a nove, sendo que 63,00% dos ramos apresentaram de uma a três folhas infestadas.

The Surinam cherry, Eugenia uniflora L. (Myrtaceae), is a native fruit tree of South America and it can be found throughout the Brazilian territory, as well as in Paraguay, Uruguay and Argentina. Galls induced by Eugeniamyia dispar (E. dispar) are found on the leaves of these trees. This study aims at determining the parasitoids associated to the E. dispar, the population dynamic of both E. dispar and parasitoids and the plant gall distribution. Branches and leaves were collected every two weeks during the 2007/08 and 2008/09 crops in Surinam cherry orchards situated in the city of Pelotas, state of Rio Grande do Sul, and taken to the laboratory for gall counting. Out of the collected material, ten leaves were isolated per sample into plastic containers (200ml) with 30ml of agar solution (2%) and methylparahydroxybenzoate (nipagin) (0.2%). The containers were closed and kept under temperature-controlled conditions (24±2ºC), relative humidity (70±10%) and photophase (14h). The number of emerged insects was daily reported. As a result, one species of Hymenoptera, Rileya hegeli (Eurytomidae) as a parasitoid of E. dispar was obtained. In relation to the population dynamics of the E. dispar and of the R. hegeli, during two years of evaluation it was noticed that the population of both species had an increase from October, and the largest populations were achieved in December, January and February, and then, decreasing onward. The number of galls per leave varied from zero to 23 and 61.19% of the leaves had one to eight galls. The number of leaves with galls in a branch varied from one to nine, and 63.00% of the branches had one to three infested leaves.

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11.

Arcobacter butzleri: first isolation report from chicken carcasses in costa rica
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Arias, Maria Laura

; Cid, Adriana

; Fernandéz, Heriberto

Brazilian Journal of Microbiology

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jun 2011, Volume 42 N. 2 Pages 703 - 706

Abstract: En | Text: En | PDF: En

Arcobacter butzleri isolation from chicken carcasses in Costa Rica is reported for the first time. The isolated strains (P and R) were presumptively identified by their phenotypic characteristics. Definitive identification was made using a multiplex PCR assay for the simultaneous detection and identification of Arcobacter butzleri, Arcobacter cryaerophilus and Arcobacter skirrowii. These first isolations indicate the necessity of further investigation about the prevalence, distribution, ecology and interactions with human beings of this and other Arcobacter species.

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